Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.389
Hudaini Hasbi, H. Sonjaya, S. Gustina
The present study aims to determine the potential of Bali cattle ovaries as sources of oocytes for in vitro embryo production based on different ovarian reproductive statuses. The ovaries were grouped into 4 categories: ovaries with no corpus luteum and dominant follicles (CL-DF-), those with corpus luteum and no dominant follicles (CL+DF-), those without corpus luteum but with dominant follicles (CL-DF+), and those with corpus luteum and dominant follicle (CL+DF+). The oocytes were collected via the slicing technique and grouped into 4 grades (a, b, c, and d). The oocyte’s maturation was performed using tissue culture medium 199 basic media. A drop sample (10–15 oocytes/drop) covered with mineral oil was then placed in a 5% CO 2 incubator at a temperature of 38.5 ℃ for 24 h. Then, the samples were fertilized in 80 μL of fertilization medium with a final spermatozoa concentration of 1.5×10 6 spermatozoa/mL. After 5–6 h of in vitro fertilization, the oocytes were washed four times using the Charles Rosenkrans 1aa (CR1aa) medium. Then, the samples were cultured using the CR1aa as a base medium. The results showed no significant difference (p>0.05) for the 4 groups based on the oocyte population collected from one pair ovary as well as the number of oocytes that were suitable for maturation. However, group CL-DF+ showed a significant difference (p<0.05) in the rate of nuclear maturation (80.00±12.84), fertilization rate (80.00±4.72), and the ability of embryo development (60.19±22.45) when compared to group CL-DF-, CL+DF, and CL+DF+. This study determines that the oocyte population of Bali cattle ovary pairs and oocytes quantity that are fit for maturation is not influenced by the reproductive status of the ovaries. However, the level of nuclear maturation, fertilization, and the ability of embryo development is higher in the ovaries without corpus luteum but with dominant follicles.
{"title":"Oocytes Population and Development Competence of Bali Cattle Embryo In Vitro with Different Ovarian Reproductive Statuses","authors":"Hudaini Hasbi, H. Sonjaya, S. Gustina","doi":"10.5398/tasj.2022.45.4.389","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.389","url":null,"abstract":"The present study aims to determine the potential of Bali cattle ovaries as sources of oocytes for in vitro embryo production based on different ovarian reproductive statuses. The ovaries were grouped into 4 categories: ovaries with no corpus luteum and dominant follicles (CL-DF-), those with corpus luteum and no dominant follicles (CL+DF-), those without corpus luteum but with dominant follicles (CL-DF+), and those with corpus luteum and dominant follicle (CL+DF+). The oocytes were collected via the slicing technique and grouped into 4 grades (a, b, c, and d). The oocyte’s maturation was performed using tissue culture medium 199 basic media. A drop sample (10–15 oocytes/drop) covered with mineral oil was then placed in a 5% CO 2 incubator at a temperature of 38.5 ℃ for 24 h. Then, the samples were fertilized in 80 μL of fertilization medium with a final spermatozoa concentration of 1.5×10 6 spermatozoa/mL. After 5–6 h of in vitro fertilization, the oocytes were washed four times using the Charles Rosenkrans 1aa (CR1aa) medium. Then, the samples were cultured using the CR1aa as a base medium. The results showed no significant difference (p>0.05) for the 4 groups based on the oocyte population collected from one pair ovary as well as the number of oocytes that were suitable for maturation. However, group CL-DF+ showed a significant difference (p<0.05) in the rate of nuclear maturation (80.00±12.84), fertilization rate (80.00±4.72), and the ability of embryo development (60.19±22.45) when compared to group CL-DF-, CL+DF, and CL+DF+. This study determines that the oocyte population of Bali cattle ovary pairs and oocytes quantity that are fit for maturation is not influenced by the reproductive status of the ovaries. However, the level of nuclear maturation, fertilization, and the ability of embryo development is higher in the ovaries without corpus luteum but with dominant follicles.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49668879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.490
S. P. Syahlani, F. Haryadi, A. Setyawan, I. Mayasari, N. K. Dewi, N. Qui
Regulatory changes increase the competitiveness of agribusiness input market, thus requiring input supply companies to improve service quality to maintain customer loyalty. This study aims to determine the role of customer comfort, salesperson expertise, and salesperson affection to influence customer satisfaction and delight and the effects on repurchase intention in poultry agribusiness context. Respondents were selected through judgmental sampling method who were the farmers who buy certain brand of poultry input for farm production process. Data were collected through survey using self-reported of questionnaire. The data obtained were tested for validity and reliability and examined by path analysis with Partial Least Square software. The results showed that customer comfort and customer affection influenced customer satisfaction and customer delight. However, salesperson expertise did not influence customer satisfaction and delight. Furthermore, repurchase intention in poultry farming input market was affected by customer satisfaction but not by customer delight. Therefore, it can be concluded that salesperson affection was found as the most important factor and followed by customer comfort to influence repurchase intention behavior through customer satisfaction.
{"title":"Key Driver of Repurchase Intention in the Poultry Farming Input Market in Indonesia","authors":"S. P. Syahlani, F. Haryadi, A. Setyawan, I. Mayasari, N. K. Dewi, N. Qui","doi":"10.5398/tasj.2022.45.4.490","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.490","url":null,"abstract":"Regulatory changes increase the competitiveness of agribusiness input market, thus requiring input supply companies to improve service quality to maintain customer loyalty. This study aims to determine the role of customer comfort, salesperson expertise, and salesperson affection to influence customer satisfaction and delight and the effects on repurchase intention in poultry agribusiness context. Respondents were selected through judgmental sampling method who were the farmers who buy certain brand of poultry input for farm production process. Data were collected through survey using self-reported of questionnaire. The data obtained were tested for validity and reliability and examined by path analysis with Partial Least Square software. The results showed that customer comfort and customer affection influenced customer satisfaction and customer delight. However, salesperson expertise did not influence customer satisfaction and delight. Furthermore, repurchase intention in poultry farming input market was affected by customer satisfaction but not by customer delight. Therefore, it can be concluded that salesperson affection was found as the most important factor and followed by customer comfort to influence repurchase intention behavior through customer satisfaction.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47260458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.397
T. Maulana, R. Ridwan, M. Gunawan, P. P. Agung, F. Afiati, E. M. Kaiin, S. Said
{"title":"Successful Separation of X- and Y-Spermatozoa Ongole Crossbreed Using a Nano-Albumen Gradient Column","authors":"T. Maulana, R. Ridwan, M. Gunawan, P. P. Agung, F. Afiati, E. M. Kaiin, S. Said","doi":"10.5398/tasj.2022.45.4.397","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.397","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47287816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.381
Faisal Amri Satrio, N. W. Karja, M. A. Setiadi, E. M. Kaiin, M. Gunawan, B. Purwantara
{"title":"Post-Thaw Characteristics of the Simmental Sperm Function in Different Ages of Bulls","authors":"Faisal Amri Satrio, N. W. Karja, M. A. Setiadi, E. M. Kaiin, M. Gunawan, B. Purwantara","doi":"10.5398/tasj.2022.45.4.381","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.381","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44066478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.413
M. Hatami, H. M. Yarahmadi, J. Fakhraei, A. Ahmadi
This study was performed to evaluate the effects of vitamin E, L-carnitine, and fish oil on the numbers of follicles, pregnancy, Reactive Oxygen Species (ROS) expression, and expression of several ovarian genes in sheep. For this purpose, 256 sheep were randomly divided into eight experimental groups. They were given a diet supplemented with vitamin E, L-carnitine, and fish oil alone and in combination. FBS, estradiol levels, size of the follicles, and pregnancy rate were observed. Expressions of Superoxide Dismutase 1 ( SOD1 ), Superoxide Dismutase 2 ( SOD2 ), Transforming Growth Factor –β ( TGF-β ), and Peroxisome Proliferator-Activated Gamma Receptor ( PPAR-γ ) genes were measured using Reverse Transcription-Polymerase Chain Reaction (RT-PCR), and ROS was measured using fluorescence microscope. At the time of mating and pregnancy, the highest number of follicles and pregnant ewes were observed in the groups supplemented with fish oil and antioxidants (p<0.01). The lowest numbers of follicles and pregnancy was observed in the group supplemented with fish oil alone (p<0.01). The highest expressions of SOD1 and TGF-β genes (p<0.01) as well as SOD2 and PPAR-γ (p<0.05) were observed in the group of concomitant use of fish oil with antioxidants. The highest amount of ROS was found in fish oil group (p<0.01), and the lowest was found in the groups supplemented with fish oil and antioxidant (p<0.01). The use of fish oil along with vitamin E and L-carnitine improved follicle function and increased pregnancy rate by reducing ROS in ewes’ ovaries as well as increasing the expression of SOD1 , SOD2 , TGF-βRI , and PPAR-γ genes. The use of fish oil along with antioxidants increases follicles and improves fertility in sheep.
{"title":"Numbers of Follicles, Pregnancy, Expression of Reactive Oxygen Species (ROS), and Ovarian Genes in Sheep Treated with Vitamin E, L-Carnitine, and Fish Oil","authors":"M. Hatami, H. M. Yarahmadi, J. Fakhraei, A. Ahmadi","doi":"10.5398/tasj.2022.45.4.413","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.413","url":null,"abstract":"This study was performed to evaluate the effects of vitamin E, L-carnitine, and fish oil on the numbers of follicles, pregnancy, Reactive Oxygen Species (ROS) expression, and expression of several ovarian genes in sheep. For this purpose, 256 sheep were randomly divided into eight experimental groups. They were given a diet supplemented with vitamin E, L-carnitine, and fish oil alone and in combination. FBS, estradiol levels, size of the follicles, and pregnancy rate were observed. Expressions of Superoxide Dismutase 1 ( SOD1 ), Superoxide Dismutase 2 ( SOD2 ), Transforming Growth Factor –β ( TGF-β ), and Peroxisome Proliferator-Activated Gamma Receptor ( PPAR-γ ) genes were measured using Reverse Transcription-Polymerase Chain Reaction (RT-PCR), and ROS was measured using fluorescence microscope. At the time of mating and pregnancy, the highest number of follicles and pregnant ewes were observed in the groups supplemented with fish oil and antioxidants (p<0.01). The lowest numbers of follicles and pregnancy was observed in the group supplemented with fish oil alone (p<0.01). The highest expressions of SOD1 and TGF-β genes (p<0.01) as well as SOD2 and PPAR-γ (p<0.05) were observed in the group of concomitant use of fish oil with antioxidants. The highest amount of ROS was found in fish oil group (p<0.01), and the lowest was found in the groups supplemented with fish oil and antioxidant (p<0.01). The use of fish oil along with vitamin E and L-carnitine improved follicle function and increased pregnancy rate by reducing ROS in ewes’ ovaries as well as increasing the expression of SOD1 , SOD2 , TGF-βRI , and PPAR-γ genes. The use of fish oil along with antioxidants increases follicles and improves fertility in sheep.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44896405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.405
H. Iskandar, H. Sonjaya, R. Arifiantini, Hudaini Hasbi
This study evaluated the quality of fresh and frozen semen of Bali cattle and its correlation with the molecular weight (MW) of seminal plasma protein. This study collected semen from 10 bulls aged 5–10 years using an artificial vagina and evaluated the samples macroscopically and microscopically. Two batches of frozen semen obtained in 2020 and 2021 were also analyzed. The frozen semen samples were thawed at 37 °C for 30 seconds. The sperm motility, viability, intact plasma membrane (IPM), and sperm abnormalities were investigated. The concentration of the seminal plasma proteins was determined using the Bradford method, and the proteins were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-SDS-PAGE). Additionally, the gels were stained with Coomassie brilliant blue, and the MWs of the proteins were determined using MW markers. The sperm motility, viability, and abnormalities of fresh semen varied significantly among the bulls (p<0.05); however, sperm IPMs among the bulls were similar (p>0.05). No differences in sperm motility after freezing were found among the bulls. However, the sperm viability, abnormality, and IPM varied among the bulls. Meanwhile, the seminal plasma proteins contained bands with different MWs. No difference in the expression of protein bands between bulls. Linearity analysis showed that sperm motility (r= 0.281), viability (r= 0.189), abnormalities (r= 0.141), and IPM (r= 0.173) were positively correlated with the protein bands at each MW (p<0.05). The results conclude there was a positive correlation between the MW of the protein marker and the same protein expression levels in Bali bulls. Therefore, the band intensity of Bali cattle seminal plasma proteins can be used as a biomarker for selecting superior Bali bulls.
{"title":"The Quality of Fresh and Frozen Semen and its Correlation with Molecular Weight of Seminal Plasma Protein in Bali Cattle","authors":"H. Iskandar, H. Sonjaya, R. Arifiantini, Hudaini Hasbi","doi":"10.5398/tasj.2022.45.4.405","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.405","url":null,"abstract":"This study evaluated the quality of fresh and frozen semen of Bali cattle and its correlation with the molecular weight (MW) of seminal plasma protein. This study collected semen from 10 bulls aged 5–10 years using an artificial vagina and evaluated the samples macroscopically and microscopically. Two batches of frozen semen obtained in 2020 and 2021 were also analyzed. The frozen semen samples were thawed at 37 °C for 30 seconds. The sperm motility, viability, intact plasma membrane (IPM), and sperm abnormalities were investigated. The concentration of the seminal plasma proteins was determined using the Bradford method, and the proteins were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-SDS-PAGE). Additionally, the gels were stained with Coomassie brilliant blue, and the MWs of the proteins were determined using MW markers. The sperm motility, viability, and abnormalities of fresh semen varied significantly among the bulls (p<0.05); however, sperm IPMs among the bulls were similar (p>0.05). No differences in sperm motility after freezing were found among the bulls. However, the sperm viability, abnormality, and IPM varied among the bulls. Meanwhile, the seminal plasma proteins contained bands with different MWs. No difference in the expression of protein bands between bulls. Linearity analysis showed that sperm motility (r= 0.281), viability (r= 0.189), abnormalities (r= 0.141), and IPM (r= 0.173) were positively correlated with the protein bands at each MW (p<0.05). The results conclude there was a positive correlation between the MW of the protein marker and the same protein expression levels in Bali bulls. Therefore, the band intensity of Bali cattle seminal plasma proteins can be used as a biomarker for selecting superior Bali bulls.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46448358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.436
K. H. Suradiradja, I. S. Sitanggang, L. Abdullah, I. Hermadi
{"title":"Estimation of Harvest Time of Forage Sorghum (Sorghum Bicolor) CV. Samurai-2 Using Decision Tree Algorithm","authors":"K. H. Suradiradja, I. S. Sitanggang, L. Abdullah, I. Hermadi","doi":"10.5398/tasj.2022.45.4.436","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.436","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47488872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.429
I. Suhendro, J. Jakaria, R. Priyanto, W. Manalu, R. R. Noor
{"title":"The Association of Single Nucleotide Polymorphism -69T>G HSPA1A Gene with Bali Cattle Heat Tolerance","authors":"I. Suhendro, J. Jakaria, R. Priyanto, W. Manalu, R. R. Noor","doi":"10.5398/tasj.2022.45.4.429","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.429","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48474685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.423
S. Santoso, A. Gunawan, C. Sumantri, R. Arifiantini, H. Herdis
{"title":"Differential Expressions of Protamine 1 (PRM1) and Protamine 2 (PRM2) Genes as Markers of Semen Quality in Pasundan Bulls","authors":"S. Santoso, A. Gunawan, C. Sumantri, R. Arifiantini, H. Herdis","doi":"10.5398/tasj.2022.45.4.423","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.423","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42420730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.5398/tasj.2022.45.4.482
S. Supratikno, H. Setijanto, H. Nuraini, S. Agungpriyono
{"title":"Bleeding Time and False Aneurysm Incidence on Cattle Slaughtering Using Non-Penetrative Pre-Slaughter Stunning in Indonesia","authors":"S. Supratikno, H. Setijanto, H. Nuraini, S. Agungpriyono","doi":"10.5398/tasj.2022.45.4.482","DOIUrl":"https://doi.org/10.5398/tasj.2022.45.4.482","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48273176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：