Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367670
D. Nikolić, M. Matović, M. Jeremić, A. Cvetkovic, S. Ninkovic, M. Kojic, N. Filipovic
Hybrid imaging combining CT and SPECT is becoming a state of the art nuclear medicine technique. Advantages of hybrid imaging are improved quality of the images using CT data for attenuation correction based on true transmission density data in an individual patient, and CT and SPECT fusion images providing accurate localization of the tracer uptake. Accurate localization of increased tracer uptake is very important especially in the diagnostic of tumors. For this purpose a software developed for generating 3D models from standard DICOM images, obtained from CT and SPECT, and then merging these 3D objects, provides us with the exact location of sentinels nodes. This is certainly very helpful for a surgeon performing biopsy. The ability of SPECT/CT to improve diagnostic accuracy, especially specificity, has a great potential in further development of nuclear medicine techniques in evaluation of tumors.
{"title":"Hybrid SPECT/MSCT 3D computational preoperative simulation in breast cancer surgery","authors":"D. Nikolić, M. Matović, M. Jeremić, A. Cvetkovic, S. Ninkovic, M. Kojic, N. Filipovic","doi":"10.1109/BIBE.2015.7367670","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367670","url":null,"abstract":"Hybrid imaging combining CT and SPECT is becoming a state of the art nuclear medicine technique. Advantages of hybrid imaging are improved quality of the images using CT data for attenuation correction based on true transmission density data in an individual patient, and CT and SPECT fusion images providing accurate localization of the tracer uptake. Accurate localization of increased tracer uptake is very important especially in the diagnostic of tumors. For this purpose a software developed for generating 3D models from standard DICOM images, obtained from CT and SPECT, and then merging these 3D objects, provides us with the exact location of sentinels nodes. This is certainly very helpful for a surgeon performing biopsy. The ability of SPECT/CT to improve diagnostic accuracy, especially specificity, has a great potential in further development of nuclear medicine techniques in evaluation of tumors.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126622217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367729
I. Gadjanski, N. Filipovic
One of the key challenges in osteochondral tissue engineering is to achieve mechanical properties in the engineered tissue that are equivalent to the native tissue. Detailed knowledge of the mechanotransduction pathways occuring in the native tissue is necessary before manipulation towards the aimed properties in the engineered tissue. Purinergic (ATP-mediated) signaling is proving to be one of the main mechanisms how the chondrogenic cells sense and respond to the mechanical stimulation. In this study we performed experiments to evaluate the mechanosensitive purinergic response of chondrocytes and chondrogenic mesenchymal stem cells and we further developed a mathematical model showing ATP release changes in loaded vs. unloaded cell constructs over time. Such model can be of value in determining the potential for pharmacological manipulation of the purinergic mechanotransduction in the engineered osteochondral tissues.
{"title":"Mathematical modeling of ATP release in response to mechanical stimulation of chondrogenic cells","authors":"I. Gadjanski, N. Filipovic","doi":"10.1109/BIBE.2015.7367729","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367729","url":null,"abstract":"One of the key challenges in osteochondral tissue engineering is to achieve mechanical properties in the engineered tissue that are equivalent to the native tissue. Detailed knowledge of the mechanotransduction pathways occuring in the native tissue is necessary before manipulation towards the aimed properties in the engineered tissue. Purinergic (ATP-mediated) signaling is proving to be one of the main mechanisms how the chondrogenic cells sense and respond to the mechanical stimulation. In this study we performed experiments to evaluate the mechanosensitive purinergic response of chondrocytes and chondrogenic mesenchymal stem cells and we further developed a mathematical model showing ATP release changes in loaded vs. unloaded cell constructs over time. Such model can be of value in determining the potential for pharmacological manipulation of the purinergic mechanotransduction in the engineered osteochondral tissues.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130707361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367646
Jelena Tošović, Z. Milosevic, S. Marković
The UV/Vis properties of 14 naturally occurring flavonoids, including flavones, flavonols, and isoflavones, were investigated. The TDDFT approach in combination with the B3LYP method and 6-311+G(d, p) basis set was applied. To avoid the Kohn-Sham orbitals, whose shapes are misleading when used to interpret and describe electronic transitions, the natural bond orbital analysis was applied. Taking into account the energy gap, spatial separation, and character of the π bonding, lone pair, and π* antibonding natural localized molecular orbitals (NLMOs), the "NLMO clusters" were constructed. NLMO cluster is a molecular moiety characterized with distinguished electron density. Our investigation showed that the TDDFT and NBO approaches are complementary, implying that the results from the two theories can be combined to better understand the redistribution of electron density upon excitation. Agreement between the predictions of the TDDFT approach and those based on the NLMO clusters is excellent in the case of major electronic transitions and small excitation energies.
{"title":"Simulation of the UV/Vis spectra of flavonoids","authors":"Jelena Tošović, Z. Milosevic, S. Marković","doi":"10.1109/BIBE.2015.7367646","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367646","url":null,"abstract":"The UV/Vis properties of 14 naturally occurring flavonoids, including flavones, flavonols, and isoflavones, were investigated. The TDDFT approach in combination with the B3LYP method and 6-311+G(d, p) basis set was applied. To avoid the Kohn-Sham orbitals, whose shapes are misleading when used to interpret and describe electronic transitions, the natural bond orbital analysis was applied. Taking into account the energy gap, spatial separation, and character of the π bonding, lone pair, and π* antibonding natural localized molecular orbitals (NLMOs), the \"NLMO clusters\" were constructed. NLMO cluster is a molecular moiety characterized with distinguished electron density. Our investigation showed that the TDDFT and NBO approaches are complementary, implying that the results from the two theories can be combined to better understand the redistribution of electron density upon excitation. Agreement between the predictions of the TDDFT approach and those based on the NLMO clusters is excellent in the case of major electronic transitions and small excitation energies.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122106972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367624
Michail Tsakalakis, N. Bourbakis
There is an undisputed need for increased portability and flexibility in diagnostic ultrasound imaging due to the continuously evolving point of care (POC) clinical applications. In order to meet this growing need numerous portable ultrasound systems have been proposed. The design of portable systems is a very challenging task, especially for volumetric imaging system which utilize transducers with hundreds and thousands of elements. This study presents a new low-cost, portable volumetric ultrasound system for ambulatory healthcare monitoring which can serve as an enhanced alternative to the existing portable systems. All hardware components along with vital processes that are performed during the image acquisition, are presented in details. Initially, the design of the proposed 2-D phased array transducer is introduced and its performance is evaluated. Secondly, all hardware components of the system are explicitly presented and finally a software-based methodology for image enhancement that is based in image fusion and a super - resolution technique is described. The imaging quality of the designed transducer is investigated through simulations using FIELD II. Additionally, preliminary simulation results of the applied methodology for 3D images, show significant image quality improvement, especially in the regions where recording from all transducers was possible.
{"title":"Designing of a low-cost, volumetric multi — Transducer phased array ultrasound system","authors":"Michail Tsakalakis, N. Bourbakis","doi":"10.1109/BIBE.2015.7367624","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367624","url":null,"abstract":"There is an undisputed need for increased portability and flexibility in diagnostic ultrasound imaging due to the continuously evolving point of care (POC) clinical applications. In order to meet this growing need numerous portable ultrasound systems have been proposed. The design of portable systems is a very challenging task, especially for volumetric imaging system which utilize transducers with hundreds and thousands of elements. This study presents a new low-cost, portable volumetric ultrasound system for ambulatory healthcare monitoring which can serve as an enhanced alternative to the existing portable systems. All hardware components along with vital processes that are performed during the image acquisition, are presented in details. Initially, the design of the proposed 2-D phased array transducer is introduced and its performance is evaluated. Secondly, all hardware components of the system are explicitly presented and finally a software-based methodology for image enhancement that is based in image fusion and a super - resolution technique is described. The imaging quality of the designed transducer is investigated through simulations using FIELD II. Additionally, preliminary simulation results of the applied methodology for 3D images, show significant image quality improvement, especially in the regions where recording from all transducers was possible.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"320 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115280606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367722
J. Radulović, N. Mijailovic, Vesna Ranković, M. Trajanovic, N. Filipovic
In this study the authors present the method for determination of exposure dose on human head during computer tomography (CT) scanning procedure. The method is based on the use of the feed-forward neural network (FFNN) model to predict the exposure dose on human head. The neural network with Levenberg-Marquardt learning is constructed. The training data are obtained using the Monte Carlo method simulation. The simulation is performed by generating random numbers for determination of photon direction and for quantification of interaction between X-ray photon and head tissue. Spectra of photon energy is used for 3DCT scanner, X-ray tube Model XRS-125-7K-P. The FFNN predicted values are in accordance with the values obtained by the simulation with correlation coefficient around 0.99.
{"title":"Modeling of radiation dose of human head during CT scanning using neural networks","authors":"J. Radulović, N. Mijailovic, Vesna Ranković, M. Trajanovic, N. Filipovic","doi":"10.1109/BIBE.2015.7367722","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367722","url":null,"abstract":"In this study the authors present the method for determination of exposure dose on human head during computer tomography (CT) scanning procedure. The method is based on the use of the feed-forward neural network (FFNN) model to predict the exposure dose on human head. The neural network with Levenberg-Marquardt learning is constructed. The training data are obtained using the Monte Carlo method simulation. The simulation is performed by generating random numbers for determination of photon direction and for quantification of interaction between X-ray photon and head tissue. Spectra of photon energy is used for 3DCT scanner, X-ray tube Model XRS-125-7K-P. The FFNN predicted values are in accordance with the values obtained by the simulation with correlation coefficient around 0.99.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129723661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367662
L. Koumakis, G. Potamias, K. Marias, M. Tsiknakis
Demand for analyzing very large datasets is increasing, especially with the introduction of chromatin immunoprecipitation sequencing which is a recent method of Next Generation Sequencing used to analyze protein interactions with DNA. The development of new technologies is revolutionizing genome-wide analysis and scientists' abilities to have a better understanding of the biological meaning but inferring gene regulatory networks from such data is still a major challenge in systems biology. Complex reactions at the molecular level in living cells and such knowledge, as it relates to specific phenotype, necessarily implies that a key molecular target should be considered within the framework of its gene regulatory network. The objective of our study is to explore the effect of proteins under specific conditions (e.g. treatment or starvation), in functional sub-pathways for specific phenotype. Using public microarray expression datasets for glioma and the KEGG human gene regulatory networks as proof of concept, we identified disrupted sub-paths due to STAT3 on functional glioma pathways. We expect that the proposed algorithmic approach could aid researchers to determine the biological relevance of the binding sites over functional sub-paths and provide insights for new disease treatments.
{"title":"An algorithmic approach for the effect of transcription factor binding sites over functional gene regulatory networks","authors":"L. Koumakis, G. Potamias, K. Marias, M. Tsiknakis","doi":"10.1109/BIBE.2015.7367662","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367662","url":null,"abstract":"Demand for analyzing very large datasets is increasing, especially with the introduction of chromatin immunoprecipitation sequencing which is a recent method of Next Generation Sequencing used to analyze protein interactions with DNA. The development of new technologies is revolutionizing genome-wide analysis and scientists' abilities to have a better understanding of the biological meaning but inferring gene regulatory networks from such data is still a major challenge in systems biology. Complex reactions at the molecular level in living cells and such knowledge, as it relates to specific phenotype, necessarily implies that a key molecular target should be considered within the framework of its gene regulatory network. The objective of our study is to explore the effect of proteins under specific conditions (e.g. treatment or starvation), in functional sub-pathways for specific phenotype. Using public microarray expression datasets for glioma and the KEGG human gene regulatory networks as proof of concept, we identified disrupted sub-paths due to STAT3 on functional glioma pathways. We expect that the proposed algorithmic approach could aid researchers to determine the biological relevance of the binding sites over functional sub-paths and provide insights for new disease treatments.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129035270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367730
F. Canbay, Vecdi Emre Levent, Gorkem Serbes, H. F. Ugurdag, Sezer Gören, N. Aydin
Biomedical signals (BSs), which give information about the normal condition and also the inherent irregularities of our body, are expected to have non-stationary character due to the time-varying behavior of physiological systems. The Fourier transform and the short time Fourier transform are the widely used frequency and time-frequency analysis methods for extracting information from BSs with fixed frequency and time-frequency resolution respectively. However, in order to derive relevant information from non-stationary BSs, an appropriate analysis method which exhibits adjustable time-frequency resolution is needed. The wavelet transform (WT) can be used as a mathematical microscope in which the time-frequency resolution can be adjusted according to the different parts of the signal. The discrete wavelet transform (DWT) is a fast and discretized implementation for classical WT. Due to the aliasing, lack of directionality and shift-variance disadvantages, the DWT exhibits limited performance in the process of BSs. In literature, an improved version of the DWT, which is named as Dual Tree Complex Wavelet Transform (DTCWT), is employed in the analysis of BSs with great success. In this study, considering the improvements in embedded system technology and the needs for wavelet based real-time feature extraction or de-noising systems in portable medical devices, the DTCWT is implemented as a sub-system in field programmable gate arrays. In proposed hardware architecture, for every data input-channel, DTCWT is implemented by using only one adder and one multiplier. Additionally, considering the multi-channel outputs of biomedical data acquisition systems, this architecture is designed with the capability of running in parallel for N channels.
生物医学信号(BSs)提供了关于我们身体正常状态和固有不规则性的信息，由于生理系统的时变行为，生物医学信号被期望具有非平稳特性。傅里叶变换和短时傅里叶变换分别是固定频率和时频分辨率下广泛使用的频率和时频分析方法。然而，为了从非平稳BSs中获得相关信息，需要一种适当的时频分辨率可调的分析方法。小波变换可以作为一个数学显微镜，它的时频分辨率可以根据信号的不同部分进行调整。离散小波变换(DWT)是对经典小波变换的一种快速、离散化实现，但由于存在混叠、方向性不足和移位方差等缺点，使得DWT在BSs处理中表现有限。在文献中，将DWT的改进版本称为对偶树复小波变换(Dual Tree Complex Wavelet Transform, DTCWT)用于分析BSs，并取得了很大的成功。在本研究中，考虑到嵌入式系统技术的进步和便携式医疗设备对基于小波的实时特征提取或去噪系统的需求，DTCWT作为一个子系统在现场可编程门阵列中实现。在提出的硬件架构中，对于每个数据输入通道，DTCWT仅使用一个加法器和一个乘法器来实现。此外，考虑到生物医学数据采集系统的多通道输出，该架构设计具有N通道并行运行的能力。
{"title":"An area efficient real time implementation of dual tree complex wavelet transform in field programmable gate arrays","authors":"F. Canbay, Vecdi Emre Levent, Gorkem Serbes, H. F. Ugurdag, Sezer Gören, N. Aydin","doi":"10.1109/BIBE.2015.7367730","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367730","url":null,"abstract":"Biomedical signals (BSs), which give information about the normal condition and also the inherent irregularities of our body, are expected to have non-stationary character due to the time-varying behavior of physiological systems. The Fourier transform and the short time Fourier transform are the widely used frequency and time-frequency analysis methods for extracting information from BSs with fixed frequency and time-frequency resolution respectively. However, in order to derive relevant information from non-stationary BSs, an appropriate analysis method which exhibits adjustable time-frequency resolution is needed. The wavelet transform (WT) can be used as a mathematical microscope in which the time-frequency resolution can be adjusted according to the different parts of the signal. The discrete wavelet transform (DWT) is a fast and discretized implementation for classical WT. Due to the aliasing, lack of directionality and shift-variance disadvantages, the DWT exhibits limited performance in the process of BSs. In literature, an improved version of the DWT, which is named as Dual Tree Complex Wavelet Transform (DTCWT), is employed in the analysis of BSs with great success. In this study, considering the improvements in embedded system technology and the needs for wavelet based real-time feature extraction or de-noising systems in portable medical devices, the DTCWT is implemented as a sub-system in field programmable gate arrays. In proposed hardware architecture, for every data input-channel, DTCWT is implemented by using only one adder and one multiplier. Additionally, considering the multi-channel outputs of biomedical data acquisition systems, this architecture is designed with the capability of running in parallel for N channels.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127568343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367725
Suzana Petrovic, G. Devedžić, B. Ristic, A. Matic, N. Prodanović
In studies so far have been shown that clinical analysis of the gait parameters is extremely important for improving diagnostics and following illness or deficiencies of lower extremities, as well as for analyzing recuperation levels after therapy. Purpose of this paper is to analyze possibility of using easily affordable web cameras for recording knee movement. Recordings are made with web cam on Fujitsu lifebook E laptop. Algorithm for object tracking is made in programming environment MatLAB. Five participants have participated in this research. Results are graphically presented for left and right leg for one gait cycle along straight line and values for anterior posterior (AP) translation are shown in tables for every phase of gait cycle. Acquired study results show that it is possible to measure observed parameter in this way and that values correspond with values obtained in previous studies.
{"title":"Gait parameters identification using video tracking — Case study","authors":"Suzana Petrovic, G. Devedžić, B. Ristic, A. Matic, N. Prodanović","doi":"10.1109/BIBE.2015.7367725","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367725","url":null,"abstract":"In studies so far have been shown that clinical analysis of the gait parameters is extremely important for improving diagnostics and following illness or deficiencies of lower extremities, as well as for analyzing recuperation levels after therapy. Purpose of this paper is to analyze possibility of using easily affordable web cameras for recording knee movement. Recordings are made with web cam on Fujitsu lifebook E laptop. Algorithm for object tracking is made in programming environment MatLAB. Five participants have participated in this research. Results are graphically presented for left and right leg for one gait cycle along straight line and values for anterior posterior (AP) translation are shown in tables for every phase of gait cycle. Acquired study results show that it is possible to measure observed parameter in this way and that values correspond with values obtained in previous studies.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124499394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367671
P. Duchez, L. Rodriguez, I. Gadjanski, Z. Ivanovic
In order to facilitate the transfer of our technique of expansion of cord blood hematopoietic cells to the industrial scale, we investigated if our two-step ex vivo expansion cultures can be transformed in one-step cultures. For this purpose, the cultures are initiated with the definitive medium volume and with the cell concentrations proportionally decreased. It turned out that the one-step cultures have different kinetics of cell expansion comparing to two-step cultures. However, the one-step cultures can produce more committed progenitors if maintained for 12 days than two-step ones, which can be a non-negligible advantage. To use these cultures in clinical trials it is necessary to check the activity of the hematopoietic stem cells after expansion using in vivo repopulation models.
{"title":"Ex vivo amplification kinetics of cord blood hematopoietic progenitor cells in one- and two-step hypoxic response-mimicking cultures (HRMC)","authors":"P. Duchez, L. Rodriguez, I. Gadjanski, Z. Ivanovic","doi":"10.1109/BIBE.2015.7367671","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367671","url":null,"abstract":"In order to facilitate the transfer of our technique of expansion of cord blood hematopoietic cells to the industrial scale, we investigated if our two-step ex vivo expansion cultures can be transformed in one-step cultures. For this purpose, the cultures are initiated with the definitive medium volume and with the cell concentrations proportionally decreased. It turned out that the one-step cultures have different kinetics of cell expansion comparing to two-step cultures. However, the one-step cultures can produce more committed progenitors if maintained for 12 days than two-step ones, which can be a non-negligible advantage. To use these cultures in clinical trials it is necessary to check the activity of the hematopoietic stem cells after expansion using in vivo repopulation models.","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124113794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-02DOI: 10.1109/BIBE.2015.7367667
V. Isailović, Milica G. Nikolić, D. Nikolić, I. Šaveljić, N. Filipovic
Human hearing system in general, and particularly the cochlea, is very interesting for investigation. The most important reason for it is hearing loss - a health problem that affects a large part of the world's human population. The highest percentage of people with hearing problems are older people, but the problem also occurs in newborns. Experimental research in this area provides some information about the level of hearing loss. Therefore, it is very useful to have a numerical model of the hearing system that can significantly contribute to the understanding of the origin of the mentioned health problem. Two numerical models are developed to investigate hearing problems: passive 3D cochlea model and 2D cochlea cross-section model. Those models are weakly coupled in order to make an active cochlea model [1].
{"title":"Using of finite element method for modeling of active cochlea","authors":"V. Isailović, Milica G. Nikolić, D. Nikolić, I. Šaveljić, N. Filipovic","doi":"10.1109/BIBE.2015.7367667","DOIUrl":"https://doi.org/10.1109/BIBE.2015.7367667","url":null,"abstract":"Human hearing system in general, and particularly the cochlea, is very interesting for investigation. The most important reason for it is hearing loss - a health problem that affects a large part of the world's human population. The highest percentage of people with hearing problems are older people, but the problem also occurs in newborns. Experimental research in this area provides some information about the level of hearing loss. Therefore, it is very useful to have a numerical model of the hearing system that can significantly contribute to the understanding of the origin of the mentioned health problem. Two numerical models are developed to investigate hearing problems: passive 3D cochlea model and 2D cochlea cross-section model. Those models are weakly coupled in order to make an active cochlea model [1].","PeriodicalId":422807,"journal":{"name":"2015 IEEE 15th International Conference on Bioinformatics and Bioengineering (BIBE)","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128031338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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