Background Type 2 diabetes mellitus (T2DM) is a chronic disease that affects millions of people worldwide and associated with an increased risk of kidney damage caused by prolonged inflammation. Secretome hypoxia- mesenchymal stem cells (SH-MSCs) have been investigated as a potential therapy for kidney inflammation in T2DM, due to their immunomodulatory properties and ability to promote tissue repair. In this study, we investigated the effects of SH-MSCs on tumor necrosis α (TNF-á) and interleukin-18 (IL-18) in the kidney of the T2DM model rats. MethodsA post-test-only control group involving 24 male Wistar rats. The rats were treated with a high-fat diet (HFD) for 4 weeks and streptozotocin-nicotinamide with sucrose solution for 5 days to induce T2DM animal models. Rats were randomly divided into four groups: healthy, control, and groups treated with SH-MSCs T1 and T2, with doses of 250 µL and 500 µL, respectively. TNF-α and IL-18 gene expression was measured by real time polymerase chain reaction (RT-PCR). One Way ANOVA and post-hoc LSD tests were used to determine the significant difference against all groups based on their quantitative measurement. ResultsAdministration of the SH-MSCs at a dose of 500µL (T2) was able to significantly reduce TNF-α and IL-18 gene expression when compared to control (T2DM rat without treatment) (p<0.05), but not significantly when compared to healthy and SH-MSC at a dose of 250µL (T1) group (p >0.05). ConclusionThis study demonstrated that the SH-MSCs decreased the levels of proinflammatory cytokines TNF-α and IL-18 gene expression in the kidney of T2DM model rats.
{"title":"Secretome hypoxia-mesenchymal stem cells decrease tumor necrosis factor-α and interleukin-18 in kidney of type 2 diabetes mellitus model rats","authors":"Risky Chandra Satria Irawan, Agung Putra, Trisnadi Setyo, Shabrina Syifa Ghaisani, Nurul Hidayah","doi":"10.18051/univmed.2023.v42.320-328","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.320-328","url":null,"abstract":"Background Type 2 diabetes mellitus (T2DM) is a chronic disease that affects millions of people worldwide and associated with an increased risk of kidney damage caused by prolonged inflammation. Secretome hypoxia- mesenchymal stem cells (SH-MSCs) have been investigated as a potential therapy for kidney inflammation in T2DM, due to their immunomodulatory properties and ability to promote tissue repair. In this study, we investigated the effects of SH-MSCs on tumor necrosis α (TNF-á) and interleukin-18 (IL-18) in the kidney of the T2DM model rats. MethodsA post-test-only control group involving 24 male Wistar rats. The rats were treated with a high-fat diet (HFD) for 4 weeks and streptozotocin-nicotinamide with sucrose solution for 5 days to induce T2DM animal models. Rats were randomly divided into four groups: healthy, control, and groups treated with SH-MSCs T1 and T2, with doses of 250 µL and 500 µL, respectively. TNF-α and IL-18 gene expression was measured by real time polymerase chain reaction (RT-PCR). One Way ANOVA and post-hoc LSD tests were used to determine the significant difference against all groups based on their quantitative measurement. ResultsAdministration of the SH-MSCs at a dose of 500µL (T2) was able to significantly reduce TNF-α and IL-18 gene expression when compared to control (T2DM rat without treatment) (p<0.05), but not significantly when compared to healthy and SH-MSC at a dose of 250µL (T1) group (p >0.05). ConclusionThis study demonstrated that the SH-MSCs decreased the levels of proinflammatory cytokines TNF-α and IL-18 gene expression in the kidney of T2DM model rats.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"16 4","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139162916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-21DOI: 10.18051/univmed.2023.v42.346-359
Maria Veronica Putri Suyanto, G. Widianti, Maria Veronica, Putri Suyanto
The ultraviolet radiation of the sun that reaches the earth is made up of ultraviolet A (95%) and ultraviolet B (5%). Exposure to ultraviolet radiation (UVR) is the main factor in photoaging. Chronic exposure to sunlight acts as an environmental stressor, leading to oxidative damage or stress. Oxidative damage stimulates the accumulation of free radicals, such as reactive oxygen species (ROS) or reactive nitrogen species (RNS) that are responsible for premature skin aging. Photoaged skin is characterized externally by irregular pigmentation, wrinkles, and reduced skin elasticity, and internally by the breakdown of dermal collagen and elastin. Free radicals can be scavenged and the skin can be protected from further oxidative damage by antioxidants. Melatonin is a hormone produced mainly by the pineal gland, as well as many other organs, including the skin. One of the functions of melatonin is exerted by the antioxidative melatoninergic system to prevent ultraviolet (UV)-induced skin photoaging. The aim of this review was to study the protective effect of melatonin on skin photoaging resulting from UVR exposure. The references were tracked using various databases, such as Google Scholar and PubMed with regard to publications in English for the last 5 years (2019-2023). Melatonin inhibits UVR-induced aging in multiple ways, such as protecting skin cells, binding free radicals, accelerating the activity of antioxidant enzymes, preventing increased mitochondrial membrane permeability, and assisting electron transport efficiency in the mitochondrial respiratory chain. Exogenous application of melatonin is usually by the oral route, but for localized effects on the skin, topical administration is recommended, with consideration of preparations with a better half-life and bioavailability. Understanding the protective antioxidant function of melatonin in UV-induced skin photoaging helps to optimize its application. The protective properties of melatonin against UVR-oxidative stress-induced photoaging will be further explored in this review.
{"title":"Protective role of melatonin in ultraviolet radiation- induced oxidative stress in human skin photoaging","authors":"Maria Veronica Putri Suyanto, G. Widianti, Maria Veronica, Putri Suyanto","doi":"10.18051/univmed.2023.v42.346-359","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.346-359","url":null,"abstract":"The ultraviolet radiation of the sun that reaches the earth is made up of ultraviolet A (95%) and ultraviolet B (5%). Exposure to ultraviolet radiation (UVR) is the main factor in photoaging. Chronic exposure to sunlight acts as an environmental stressor, leading to oxidative damage or stress. Oxidative damage stimulates the accumulation of free radicals, such as reactive oxygen species (ROS) or reactive nitrogen species (RNS) that are responsible for premature skin aging. Photoaged skin is characterized externally by irregular pigmentation, wrinkles, and reduced skin elasticity, and internally by the breakdown of dermal collagen and elastin. Free radicals can be scavenged and the skin can be protected from further oxidative damage by antioxidants. Melatonin is a hormone produced mainly by the pineal gland, as well as many other organs, including the skin. One of the functions of melatonin is exerted by the antioxidative melatoninergic system to prevent ultraviolet (UV)-induced skin photoaging. The aim of this review was to study the protective effect of melatonin on skin photoaging resulting from UVR exposure. The references were tracked using various databases, such as Google Scholar and PubMed with regard to publications in English for the last 5 years (2019-2023). Melatonin inhibits UVR-induced aging in multiple ways, such as protecting skin cells, binding free radicals, accelerating the activity of antioxidant enzymes, preventing increased mitochondrial membrane permeability, and assisting electron transport efficiency in the mitochondrial respiratory chain. Exogenous application of melatonin is usually by the oral route, but for localized effects on the skin, topical administration is recommended, with consideration of preparations with a better half-life and bioavailability. Understanding the protective antioxidant function of melatonin in UV-induced skin photoaging helps to optimize its application. The protective properties of melatonin against UVR-oxidative stress-induced photoaging will be further explored in this review.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"15 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139167846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-18DOI: 10.18051/univmed.2023.v42.314-319
Siska Siska, I. R. Suryaningrum, Saphira Evani
Background Advancements in ophthalmic surgery now hinge on intricate interplays among ocular parameters. Angle kappa, measuring deviation between visual and pupillary axes, is crucial, especially in refractive procedures with multifocal intraocular lens implants. The research aimed to correlate angle kappa with biometry and higher-order aberrations (HOA) to enhance surgical outcomes among adult cataract patients at Prof. Ngoerah Hospital Ophthalmology Clinic, Denpasar, Bali. MethodsThis cross-sectional study included 29 male and female cataract patients aged 18-80 years, without prior treatment. All patients had a basic examination that included testing of visual acuity using Snellen chart, autorefractometer, measurement of ocular pressure using non-contact tonometry, and slit-lamp examination for cataract grading. Patients who met the inclusion criteria were then examined for biometry (axial length, spherical equivalent, white-to-white distance, anterior chamber depth) using Nidek AL Scan and for angle kappa and HOA using OPD scan III. ResultsData from 50 eyes of 29 subjects (15 females and 14 males) were analyzed. The mean age of the subjects was 60.6 ± 12.5 years. Age and spherical equivalent had positive correlation with angle kappa (r =0.104, r=0.213), but the correlation was not statistically significant. In this study, interestingly angle kappa was not significantly correlated with HOA, AXL, WTW, and ACD (r = -0.050, r = -0.192, r = -0.104, r = -0.195, p >0.05). ConclusionIn conclusion, angle kappa may increase with age and spherical equivalent. Further study with larger sample size is required.
{"title":"Correlation of angle kappa with biometry and higher-order aberrations of cataract patients at Prof. Ngoerah Hospital Ophthalmology Clinic","authors":"Siska Siska, I. R. Suryaningrum, Saphira Evani","doi":"10.18051/univmed.2023.v42.314-319","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.314-319","url":null,"abstract":"Background Advancements in ophthalmic surgery now hinge on intricate interplays among ocular parameters. Angle kappa, measuring deviation between visual and pupillary axes, is crucial, especially in refractive procedures with multifocal intraocular lens implants. The research aimed to correlate angle kappa with biometry and higher-order aberrations (HOA) to enhance surgical outcomes among adult cataract patients at Prof. Ngoerah Hospital Ophthalmology Clinic, Denpasar, Bali. MethodsThis cross-sectional study included 29 male and female cataract patients aged 18-80 years, without prior treatment. All patients had a basic examination that included testing of visual acuity using Snellen chart, autorefractometer, measurement of ocular pressure using non-contact tonometry, and slit-lamp examination for cataract grading. Patients who met the inclusion criteria were then examined for biometry (axial length, spherical equivalent, white-to-white distance, anterior chamber depth) using Nidek AL Scan and for angle kappa and HOA using OPD scan III. ResultsData from 50 eyes of 29 subjects (15 females and 14 males) were analyzed. The mean age of the subjects was 60.6 ± 12.5 years. Age and spherical equivalent had positive correlation with angle kappa (r =0.104, r=0.213), but the correlation was not statistically significant. In this study, interestingly angle kappa was not significantly correlated with HOA, AXL, WTW, and ACD (r = -0.050, r = -0.192, r = -0.104, r = -0.195, p >0.05). ConclusionIn conclusion, angle kappa may increase with age and spherical equivalent. Further study with larger sample size is required.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"50 ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-15DOI: 10.18051/univmed.2023.v42.368-373
Komang Agung Irianto, Enrico Leonarto, H. R. Handoyo
BackgroundCheckrein deformity is a rare abnormality comprising flexor hallucis longus (FHL) tendon entrapment in the posterior foot due to post-traumatic or ischemic retraction of the FHLtendon following soft tissue trauma. The diagnosis is essentially clinical, but complemented by imaging to rule out unrecognized causes and evaluate the fracture healing process. This case report is the first Indonesian study to describe release of the flexor retinaculum and Z-plasty (lengthening) of flexor digitorum longus (FDL) and FHL tendons through a medial retromalleolar approach to repair the deformity. Case DescriptionWe present the case of a 51-year-old male who complained of clawing of his right first and second toes. The interest of this study lies in the fact that this patient had never experienced ankle or distal tibial fractures, only a trivial injury two years back when the patient kicked a stone at work and had a bruise on his right second toe with no fracture. Exploration of FHL tendons was performed at the level of the midfoot. The patient was planned for surgery with a medial retromalleolar ankle approach, which facilitates the release of the flexor retinaculum, in conjunction with a Z-tenotomy on the FHL and FDL tendons. ConclusionA correction was achieved, and two months post-surgery there were no recurrences of the deformity. However, this surgical procedure requires more cases to support an evaluation of its effectiveness. We suggest that exploration at the ankle and midfoot should be the primary surgical intervention in similar cases of checkrein deformity.
{"title":"Release of retromalleolar flexor retinaculum and combined flexor digitorum longus and flexor hallucis longus Z-plasty in checkrein deformity: a case report","authors":"Komang Agung Irianto, Enrico Leonarto, H. R. Handoyo","doi":"10.18051/univmed.2023.v42.368-373","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.368-373","url":null,"abstract":"BackgroundCheckrein deformity is a rare abnormality comprising flexor hallucis longus (FHL) tendon entrapment in the posterior foot due to post-traumatic or ischemic retraction of the FHLtendon following soft tissue trauma. The diagnosis is essentially clinical, but complemented by imaging to rule out unrecognized causes and evaluate the fracture healing process. This case report is the first Indonesian study to describe release of the flexor retinaculum and Z-plasty (lengthening) of flexor digitorum longus (FDL) and FHL tendons through a medial retromalleolar approach to repair the deformity. Case DescriptionWe present the case of a 51-year-old male who complained of clawing of his right first and second toes. The interest of this study lies in the fact that this patient had never experienced ankle or distal tibial fractures, only a trivial injury two years back when the patient kicked a stone at work and had a bruise on his right second toe with no fracture. Exploration of FHL tendons was performed at the level of the midfoot. The patient was planned for surgery with a medial retromalleolar ankle approach, which facilitates the release of the flexor retinaculum, in conjunction with a Z-tenotomy on the FHL and FDL tendons. ConclusionA correction was achieved, and two months post-surgery there were no recurrences of the deformity. However, this surgical procedure requires more cases to support an evaluation of its effectiveness. We suggest that exploration at the ankle and midfoot should be the primary surgical intervention in similar cases of checkrein deformity.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"277 ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139177637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background Urinary tract infections (UTIs) caused by bacteria occupy the second highest rank of common infectious diseases in the world. Empirical use of antibiotics may give rise to multidrug-resistant (MDR) bacteria because of irrational prescription. Choice of antibiotics to treat UTIs is limited because of MDR bacteria. Thus, this study was conducted to investigate the bacterial antibiotic susceptibility patterns in inpatients and outpatients in Jakarta and Tangerang. Methods Bacterial isolates were obtained from midstream urine specimens from 43 inpatients and 43 outpatients with UTIs in Jakarta and Tangerang. Bacteria were isolated on blood and MacConkey agar media using colony count method. Isolate identification and their susceptibility patterns were performed using VITEK2 compact system according to manufacturers’ instructions. Data were analyzed using Chi-square test. Results A total of 89 bacterial isolates consisting of 15 bacterial species were successfully isolated from 86 specimens. Gram-negative bacteria were the most common etiology of UTIs in inpatients and outpatients. MDR bacteria were found in 52 of the 89 isolates. ESBL-producing E. coli was the most common MDR bacteria. ESBL-producing E. coli and other MDR bacteria showed good susceptibility to ertapenem, meropenem, amikacin, and tigecycline. There were no significant differences regarding the MDR bacterial count in inpatients and outpatients (p=0.521). Conclusion E. coli was found to be the most common MDR bacteria causing UTIs in inpatients and outpatients in Jakarta and Tangerang. Higher resistance to many antibiotics was found in MDR bacterial isolates in inpatients compared to outpatients. MDR bacteria in outpatient UTIs were highly resistant to commonly used antibiotics.
{"title":"Profile of multidrug-resistant bacteria causing urinary tract infections in inpatients and outpatients in Jakarta and Tangerang","authors":"Yeva Rosana, Lina Herliyana, Grady Krisandi, Erike Anggraini Suwarsono","doi":"10.18051/univmed.2023.v42.303-313","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.303-313","url":null,"abstract":"Background Urinary tract infections (UTIs) caused by bacteria occupy the second highest rank of common infectious diseases in the world. Empirical use of antibiotics may give rise to multidrug-resistant (MDR) bacteria because of irrational prescription. Choice of antibiotics to treat UTIs is limited because of MDR bacteria. Thus, this study was conducted to investigate the bacterial antibiotic susceptibility patterns in inpatients and outpatients in Jakarta and Tangerang. Methods Bacterial isolates were obtained from midstream urine specimens from 43 inpatients and 43 outpatients with UTIs in Jakarta and Tangerang. Bacteria were isolated on blood and MacConkey agar media using colony count method. Isolate identification and their susceptibility patterns were performed using VITEK2 compact system according to manufacturers’ instructions. Data were analyzed using Chi-square test. Results A total of 89 bacterial isolates consisting of 15 bacterial species were successfully isolated from 86 specimens. Gram-negative bacteria were the most common etiology of UTIs in inpatients and outpatients. MDR bacteria were found in 52 of the 89 isolates. ESBL-producing E. coli was the most common MDR bacteria. ESBL-producing E. coli and other MDR bacteria showed good susceptibility to ertapenem, meropenem, amikacin, and tigecycline. There were no significant differences regarding the MDR bacterial count in inpatients and outpatients (p=0.521). Conclusion E. coli was found to be the most common MDR bacteria causing UTIs in inpatients and outpatients in Jakarta and Tangerang. Higher resistance to many antibiotics was found in MDR bacterial isolates in inpatients compared to outpatients. MDR bacteria in outpatient UTIs were highly resistant to commonly used antibiotics.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"7 3","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-15DOI: 10.18051/univmed.2023.v42.360-367
D. E. Yulia, D. Soeharto
Background Retinopathy of prematurity (ROP) is a proliferative disease of the ocular retinal vasculature in premature infants, being a leading cause of preventable childhood blindness. The COVID-19 pandemic serves as an additional barrier to access to care and referral, potentially leading to more cases of advanced stage ROP. Although surgery can be done, ROP at this stage is highly associated with poor functional outcome irrespective of surgery. This case series aims to report advanced stage (stage 5) ROP in premature infants during the COVID-19 pandemic. MethodsA retrospective case series of medical records of paediatric patients with ROP was performed at Cipto Mangunkusumo National Referral Hospital between March 1, 2020 and December 31, 2021. This case series included new patients that presented to our clinic from March 2020 to December 2021 with stage 5 ROP. ResultsA total of 19 eyes of 10 patients with stage 5 ROP were included in this study. Mean chronologic age at presentation to our referral centre was 7.0 4.0 months. Upon presentation, all had stage 5 ROP with negative blink reflex to light, and none of the eyes in this cohort underwent surgical intervention. Mean gestational age was 30.3 3.2 weeks and mean birth weight was 1,276.7 366.4 g. ConclusionThere was an observable surge of stage 5 ROP at our referral centre with irreversible blindness. This highlights the need for proper ROP screening in all hospitals in Indonesia to prevent delay in detection and care of advanced stage ROP.
{"title":"Childhood blindness from surge of advanced stage retinopathy of prematurity in premature infants during the COVID-19 pandemic: a case series","authors":"D. E. Yulia, D. Soeharto","doi":"10.18051/univmed.2023.v42.360-367","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.360-367","url":null,"abstract":"Background Retinopathy of prematurity (ROP) is a proliferative disease of the ocular retinal vasculature in premature infants, being a leading cause of preventable childhood blindness. The COVID-19 pandemic serves as an additional barrier to access to care and referral, potentially leading to more cases of advanced stage ROP. Although surgery can be done, ROP at this stage is highly associated with poor functional outcome irrespective of surgery. This case series aims to report advanced stage (stage 5) ROP in premature infants during the COVID-19 pandemic. MethodsA retrospective case series of medical records of paediatric patients with ROP was performed at Cipto Mangunkusumo National Referral Hospital between March 1, 2020 and December 31, 2021. This case series included new patients that presented to our clinic from March 2020 to December 2021 with stage 5 ROP. ResultsA total of 19 eyes of 10 patients with stage 5 ROP were included in this study. Mean chronologic age at presentation to our referral centre was 7.0 4.0 months. Upon presentation, all had stage 5 ROP with negative blink reflex to light, and none of the eyes in this cohort underwent surgical intervention. Mean gestational age was 30.3 3.2 weeks and mean birth weight was 1,276.7 366.4 g. ConclusionThere was an observable surge of stage 5 ROP at our referral centre with irreversible blindness. This highlights the need for proper ROP screening in all hospitals in Indonesia to prevent delay in detection and care of advanced stage ROP.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"25 6","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139273324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-27DOI: 10.18051/univmed.2023.v42.294-302
Remziye Cici, G. Yılmazel, Nur Pınar Ayaz
BackgroundProstate cancer is the most prevalent malignancy among men, and psychological symptoms may affect many patients. Although much work has been carried out on loneliness, depression, and pain in geriatric prostate cancer patients, far less research has examined their associations. Therefore, the present study explored the association of loneliness and depression with pain in geriatric prostate cancer patients. MethodsA cross-sectional study was conducted involving 83 patients between the ages of 60 and 74 years, who were diagnosed with prostate cancer and were close relatives of students studying at a health sciences university. Data were collected with the Loneliness Scale for the Elderly, Beck Depression Inventory, and Numerical Rating Scale. The duration of prostate diagnosis was evaluated according to patient files. Simple and multiple logistic regression analyses were used to analyze the data. Results It was determined that the median age of the individuals included in the study was 66 years, and the median pain duration of these patients was two years. Severe pain was found in 62.7%, loneliness in 61.4%, and depression in 68.7% of the patients. Duration of diagnosis, loneliness, and depression were significantly associated with pain intensity. Depression increases the risk of pain intensity 3.41 times among patients (aOR=3.41;95% C.I.: 3.07-8.16; p=0.038) ConclusionIt was determined that duration of diagnosis, loneliness, and depression levels were risk factors for pain intensity in geriatric prostate cancer patients. Developing interventions for patients' mental states in rehabilitation studies may help alleviate the severity of pain.
{"title":"Loneliness and depression levels as risk factors of pain in geriatric prostate cancer patients","authors":"Remziye Cici, G. Yılmazel, Nur Pınar Ayaz","doi":"10.18051/univmed.2023.v42.294-302","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.294-302","url":null,"abstract":"BackgroundProstate cancer is the most prevalent malignancy among men, and psychological symptoms may affect many patients. Although much work has been carried out on loneliness, depression, and pain in geriatric prostate cancer patients, far less research has examined their associations. Therefore, the present study explored the association of loneliness and depression with pain in geriatric prostate cancer patients. MethodsA cross-sectional study was conducted involving 83 patients between the ages of 60 and 74 years, who were diagnosed with prostate cancer and were close relatives of students studying at a health sciences university. Data were collected with the Loneliness Scale for the Elderly, Beck Depression Inventory, and Numerical Rating Scale. The duration of prostate diagnosis was evaluated according to patient files. Simple and multiple logistic regression analyses were used to analyze the data. Results It was determined that the median age of the individuals included in the study was 66 years, and the median pain duration of these patients was two years. Severe pain was found in 62.7%, loneliness in 61.4%, and depression in 68.7% of the patients. Duration of diagnosis, loneliness, and depression were significantly associated with pain intensity. Depression increases the risk of pain intensity 3.41 times among patients (aOR=3.41;95% C.I.: 3.07-8.16; p=0.038) ConclusionIt was determined that duration of diagnosis, loneliness, and depression levels were risk factors for pain intensity in geriatric prostate cancer patients. Developing interventions for patients' mental states in rehabilitation studies may help alleviate the severity of pain.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"113 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-23DOI: 10.18051/univmed.2023.v42.329-345
Edy Parwanto, David Tjahyadi, Husnun Amalia, N. Hairunisa, Hosea Jaya Edy, Ashaolu Victoria Oladimeji, Noureddine Djebli
The aging process is associated with changes in hormone levels. There is a noticeable change in estrogen levels in women, while in men, there is a change in testosterone levels. In the elderly, as a result of the aging process, changes in these hormone levels affect handgrip strength, and cognitive function. In both women and men, the function of several organs is influenced by sex steroid hormones, namely Oestrogen and androgens. There are two theories of steroid action mechanism on target cells, namely steroid hormone action mechanism genomically and non-genomically. The function and action mechanism of steroid hormone is important because it is the basis of the hormonal aspect for muscle mass, handgrip strength, and cognitive function. Due to the aging process, hormonal changes in the elderly are different compared to the previous period. This has consequences for changes in metabolic processes that affect the body’s condition, including changes in the composition of bones, muscles, and other tissues, such as the brain. In the aging process, it is important to pay attention to nutritional factors because they contribute to hormone levels that help maintain muscle mass, body mass index, hand grip strength, and cognitive function. In the brain, the sex steroid hormone has activating and organizational effects mediated by intracellular or transmembrane G-protein-coupled receptors. Articles published in English in the last 9 years (from 2014 to 2023) were retrieved from Science Direct, PubMed, Springer link, Oxford and Nature using relevant searching terms. The fact that testosterone bioavailability is predominated in the brain in relation to its activity and significant positive association with processing speed, sustained attention, and working memory in older men. To obtain a better quality of life for the elderly, nutritional factors must be considered to maintain optimal sex steroid hormone levels, hand grip strength, and cognitive function.
{"title":"Role of sex steroid hormone on hand grip strength and cognitive function in the elderly","authors":"Edy Parwanto, David Tjahyadi, Husnun Amalia, N. Hairunisa, Hosea Jaya Edy, Ashaolu Victoria Oladimeji, Noureddine Djebli","doi":"10.18051/univmed.2023.v42.329-345","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.329-345","url":null,"abstract":"The aging process is associated with changes in hormone levels. There is a noticeable change in estrogen levels in women, while in men, there is a change in testosterone levels. In the elderly, as a result of the aging process, changes in these hormone levels affect handgrip strength, and cognitive function. In both women and men, the function of several organs is influenced by sex steroid hormones, namely Oestrogen and androgens. There are two theories of steroid action mechanism on target cells, namely steroid hormone action mechanism genomically and non-genomically. The function and action mechanism of steroid hormone is important because it is the basis of the hormonal aspect for muscle mass, handgrip strength, and cognitive function. Due to the aging process, hormonal changes in the elderly are different compared to the previous period. This has consequences for changes in metabolic processes that affect the body’s condition, including changes in the composition of bones, muscles, and other tissues, such as the brain. In the aging process, it is important to pay attention to nutritional factors because they contribute to hormone levels that help maintain muscle mass, body mass index, hand grip strength, and cognitive function. In the brain, the sex steroid hormone has activating and organizational effects mediated by intracellular or transmembrane G-protein-coupled receptors. Articles published in English in the last 9 years (from 2014 to 2023) were retrieved from Science Direct, PubMed, Springer link, Oxford and Nature using relevant searching terms. The fact that testosterone bioavailability is predominated in the brain in relation to its activity and significant positive association with processing speed, sustained attention, and working memory in older men. To obtain a better quality of life for the elderly, nutritional factors must be considered to maintain optimal sex steroid hormone levels, hand grip strength, and cognitive function.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139315397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-27DOI: 10.18051/univmed.2023.v42.283-293
Nur Ilham Risma Hidayati, Indra Kusuma, Eko Poerwanto
BACKGROUNDHuman dermal fibroblast (HDF) cultures can be used as a regenerative agent for wound healing. Fetal bovine serum (FBS) as a culture supplement is derived from animals, therefore not being constant in composition, causes variations in research results, thus requiring a substitute such as KnockOut serum replacement (KOSR). This study evaluated the defined KOSR as FBS substitute for HDF culture by measuring the relative expression of basic fibroblast growth factor (bFGF) and keratinocyte growth factor (KGF) messenger RNA (mRNA), HDF cell proliferation, and HDF migration. METHODSHuman dermal fibroblast culture was divided into 2 intervention groups receiving KOSR 5% and KOSR 10%, respectively, and a control group receiving FBS 10%. Reverse transcription polymerase chain reaction (RT-PCR) was used for bFGF and KGF mRNA relative expression at the fifth passage (P5). Cell counting kit-8 (CCK-8) reagent was used for the HDF cell proliferation assay at P5 and the scratch assay was used for HDF cell migration at P6. Data were analyzed using dependent t-test, One-way ANOVA, or Kruskal-Wallis test. RESULTSThere were no significant differences in bFGF and KGF mRNA relative expression and HDF migration velocity between the intervention and control groups (p>0.05 and p>0.05, respectively). The doubling time of the KOSR 5% group showed no significant difference (p>0.05), but KOSR 10% and FBS 10% showed significant differences between treatment days 2-6 and treatment days 6-10 (p<0.05). CONCLUSIONSThe KOSR 10% was comparable to FBS 10% in supporting bFGF and KGF mRNA relative expression, HDF cell proliferation, and HDF cell migration in HDF culture.
{"title":"Comparative analysis of KnockOut serum replacement and fetal bovine serum for in vitro culture of human dermal fibroblasts","authors":"Nur Ilham Risma Hidayati, Indra Kusuma, Eko Poerwanto","doi":"10.18051/univmed.2023.v42.283-293","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.283-293","url":null,"abstract":"BACKGROUNDHuman dermal fibroblast (HDF) cultures can be used as a regenerative agent for wound healing. Fetal bovine serum (FBS) as a culture supplement is derived from animals, therefore not being constant in composition, causes variations in research results, thus requiring a substitute such as KnockOut serum replacement (KOSR). This study evaluated the defined KOSR as FBS substitute for HDF culture by measuring the relative expression of basic fibroblast growth factor (bFGF) and keratinocyte growth factor (KGF) messenger RNA (mRNA), HDF cell proliferation, and HDF migration. METHODSHuman dermal fibroblast culture was divided into 2 intervention groups receiving KOSR 5% and KOSR 10%, respectively, and a control group receiving FBS 10%. Reverse transcription polymerase chain reaction (RT-PCR) was used for bFGF and KGF mRNA relative expression at the fifth passage (P5). Cell counting kit-8 (CCK-8) reagent was used for the HDF cell proliferation assay at P5 and the scratch assay was used for HDF cell migration at P6. Data were analyzed using dependent t-test, One-way ANOVA, or Kruskal-Wallis test. RESULTSThere were no significant differences in bFGF and KGF mRNA relative expression and HDF migration velocity between the intervention and control groups (p>0.05 and p>0.05, respectively). The doubling time of the KOSR 5% group showed no significant difference (p>0.05), but KOSR 10% and FBS 10% showed significant differences between treatment days 2-6 and treatment days 6-10 (p<0.05). CONCLUSIONSThe KOSR 10% was comparable to FBS 10% in supporting bFGF and KGF mRNA relative expression, HDF cell proliferation, and HDF cell migration in HDF culture.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"42 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139335569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-26DOI: 10.18051/univmed.2023.v42.276-282
Ferdy Bangun Kangsaputra, Nur Budiyono, S. R. Brodjonegoro
BackgroundUreteral stents, which are widely used after endourological procedures, cause adverse effects. However, its potential impact on sexual function remains unclear in patients undergoing ureteral stenting. This study aimed to investigate sexual function in adult male and female patients undergoing endourological procedures involving ureteral stent placement. MethodsWe conducted a prospective cohort study in a double tertiary care hospital setting with 103 selected patients (53 male and 50 female). We used a self-administered questionnaire-based survey, namely the Indonesian version of the 5-item International Index of Erectile Function (IIEF-5) for male patients and the Indonesian Female Sexual Function Index (Indonesian FSFI) for female patients at three different times (pre-stenting, four weeks after stenting, and four weeks after stent removal). A paired t-test or Wilcoxon test was used for data analysis. ResultsIn the male group, significant changes were noted in the four IIEF-5 domains and overall IIEF-5 scores at pre-stenting and four weeks after stenting (p =0.000). The female group showed significant differences for all FSFI domains and overall FSFI scores at pre-stenting and four weeks after stenting (p=0.000). Most patients in both groups recovered sexual function four weeks after stent removal, as indicated by insignificant differences in the overall IIEF-5 and FSFI scores at pre-stenting and four weeks after stent removal. ConclusionUreteral stenting led to significant but transient sexual dysfunction in both groups at four weeks after stenting. In this regard, male and female patients need to receive counselling and attention, that they may experience temporal sexual dysfunction at least for 4 weeks.
{"title":"Indwelling ureteral stenting negatively impacts sexual function transiently after endourological procedure among adult male and female patients: a prospective cohort study","authors":"Ferdy Bangun Kangsaputra, Nur Budiyono, S. R. Brodjonegoro","doi":"10.18051/univmed.2023.v42.276-282","DOIUrl":"https://doi.org/10.18051/univmed.2023.v42.276-282","url":null,"abstract":"BackgroundUreteral stents, which are widely used after endourological procedures, cause adverse effects. However, its potential impact on sexual function remains unclear in patients undergoing ureteral stenting. This study aimed to investigate sexual function in adult male and female patients undergoing endourological procedures involving ureteral stent placement. MethodsWe conducted a prospective cohort study in a double tertiary care hospital setting with 103 selected patients (53 male and 50 female). We used a self-administered questionnaire-based survey, namely the Indonesian version of the 5-item International Index of Erectile Function (IIEF-5) for male patients and the Indonesian Female Sexual Function Index (Indonesian FSFI) for female patients at three different times (pre-stenting, four weeks after stenting, and four weeks after stent removal). A paired t-test or Wilcoxon test was used for data analysis. ResultsIn the male group, significant changes were noted in the four IIEF-5 domains and overall IIEF-5 scores at pre-stenting and four weeks after stenting (p =0.000). The female group showed significant differences for all FSFI domains and overall FSFI scores at pre-stenting and four weeks after stenting (p=0.000). Most patients in both groups recovered sexual function four weeks after stent removal, as indicated by insignificant differences in the overall IIEF-5 and FSFI scores at pre-stenting and four weeks after stent removal. ConclusionUreteral stenting led to significant but transient sexual dysfunction in both groups at four weeks after stenting. In this regard, male and female patients need to receive counselling and attention, that they may experience temporal sexual dysfunction at least for 4 weeks.","PeriodicalId":42578,"journal":{"name":"Universa Medicina","volume":"11 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139336002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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