Pub Date : 2020-05-05DOI: 10.14334/wartazoa.v30i1.2473
Y. Retnani, N. Barkah, A. Saenab, .. Taryati
Feed is one of the important factor that affect livestock productivity, so the availability of good quality feed is a requirement for livestock development in an area. In the tropical area, providing forage as a crucial feed for ruminants was hampered by fluctuating seasons. Likewise, the availability of agricultural waste as an alternative source of feed material experiences the same constraints, easily damaged, and bulky (voluminous). Therefore, feed processing technology is needed so that it is become durable, easily stored, and easily provided for livestock. One way to overcome this is to utilize technology for making wafers. Information regarding technology for making animal feed wafers in Indonesia is still limited. This paper reviews a number of studies that discuss the development of wafer processing technology, types of wafers, the main components of wafer compilers, wafer manufacturing processes, reactions that occur during wafer manufacturing, nutrient quality of various wafers, and the results of research on the use of wafers for feed livestock. Wafers are feeds that are processed using heat and pressure, so that a solid, compact, and high density product is formed. Feed wafer technology is a modification of cube and block feed. Wafers are divided into feed wafers, feed supplement wafers, and complete feed wafers. Feed wafers can be used instead of concentrates. Feed supplement wafers are high in energy and protein. Complete feed wafers contain energy, protein, fiber, and complete minerals that have been adapted to the daily nutritional needs of livestock. Feed wafer processing technology can be one of the strategies to provide feed with a constant composition of nutrients throughout the season and increase production and feed efficiency.
{"title":"Processing Technology of Feed Wafer to Increase Feed Production and Efficiency","authors":"Y. Retnani, N. Barkah, A. Saenab, .. Taryati","doi":"10.14334/wartazoa.v30i1.2473","DOIUrl":"https://doi.org/10.14334/wartazoa.v30i1.2473","url":null,"abstract":"Feed is one of the important factor that affect livestock productivity, so the availability of good quality feed is a requirement for livestock development in an area. In the tropical area, providing forage as a crucial feed for ruminants was hampered by fluctuating seasons. Likewise, the availability of agricultural waste as an alternative source of feed material experiences the same constraints, easily damaged, and bulky (voluminous). Therefore, feed processing technology is needed so that it is become durable, easily stored, and easily provided for livestock. One way to overcome this is to utilize technology for making wafers. Information regarding technology for making animal feed wafers in Indonesia is still limited. This paper reviews a number of studies that discuss the development of wafer processing technology, types of wafers, the main components of wafer compilers, wafer manufacturing processes, reactions that occur during wafer manufacturing, nutrient quality of various wafers, and the results of research on the use of wafers for feed livestock. Wafers are feeds that are processed using heat and pressure, so that a solid, compact, and high density product is formed. Feed wafer technology is a modification of cube and block feed. Wafers are divided into feed wafers, feed supplement wafers, and complete feed wafers. Feed wafers can be used instead of concentrates. Feed supplement wafers are high in energy and protein. Complete feed wafers contain energy, protein, fiber, and complete minerals that have been adapted to the daily nutritional needs of livestock. Feed wafer processing technology can be one of the strategies to provide feed with a constant composition of nutrients throughout the season and increase production and feed efficiency.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"30 1","pages":"37-50"},"PeriodicalIF":0.3,"publicationDate":"2020-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41944237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-07DOI: 10.14334/wartazoa.v29i4.2063
H. Hafizuddin, N. W. Karja, L. Praharani, M. Setiadi
Reproduction is an important factor that requires attention to increase livestock production. The application of artificial insemination (AI) technology has been developed rapidly in the world, so that availability of fertile superior male is a determinant factor of successful AI. Several recent studies have been aimed to discover male fertility biomarker, by intense research on fertility-associated proteins contained in seminal plasma. This paper describes the role of adiponectin as a biomarker candidate of male fertility. Adiponectin has a positive effect on spermatozoa functionality and steroidogenesis. This has been proven by several studies that found its expression in Sertoli cells and Leydig cells in the reproductive tract. Based on the specific role on spermatozoa functionality and steroidogenesis in supporting male fertility parameter, it is strongly suggested that adiponectin is an excellent candidate biomarker for male fertility.
{"title":"Adiponectin: Potential Protein Hormone as a Candidate Biomarker for Male Fertility","authors":"H. Hafizuddin, N. W. Karja, L. Praharani, M. Setiadi","doi":"10.14334/wartazoa.v29i4.2063","DOIUrl":"https://doi.org/10.14334/wartazoa.v29i4.2063","url":null,"abstract":"Reproduction is an important factor that requires attention to increase livestock production. The application of artificial insemination (AI) technology has been developed rapidly in the world, so that availability of fertile superior male is a determinant factor of successful AI. Several recent studies have been aimed to discover male fertility biomarker, by intense research on fertility-associated proteins contained in seminal plasma. This paper describes the role of adiponectin as a biomarker candidate of male fertility. Adiponectin has a positive effect on spermatozoa functionality and steroidogenesis. This has been proven by several studies that found its expression in Sertoli cells and Leydig cells in the reproductive tract. Based on the specific role on spermatozoa functionality and steroidogenesis in supporting male fertility parameter, it is strongly suggested that adiponectin is an excellent candidate biomarker for male fertility.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"29 1","pages":"183-192"},"PeriodicalIF":0.3,"publicationDate":"2019-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46688199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-07DOI: 10.14334/wartazoa.v29i4.2077
N. Qomariyah, Y. Retnani, A. Jayanegara, E. Wina, I. Permana
Biochar and liquid smoke may be utilized as feed additives that potentially used as substitutes for antibiotics. These products are derived from the pyrolysis process that utilizes agricultural, plantation and wood residues. This paper aims to review research results regarding the use of biochar and liquid smoke as feed additives in both non-ruminants and ruminants. Information on the use of of biochar and liquid smoke in livestock are available such as the use of 0.5-1 g bamboo charcoal/ kg goat feed increased growth; addition of 0.2-0.6% corn cobs char to chicken feed showed significant increase in body weight; the addition of 0.6% biochar/ kg feed to local cattle feed increased body weight; the use of activated charcoal containing wood vinegar liquid can reduce cryptosporidiosis in goats and cattle. The opportunity to use agricultural and plantation residues as raw materials for generating biochar and liquid smoke is one of the breakthroughs in realizing the concept of sustainable and environmentally friendly of bioindustrial agriculture. The use of biochar and liquid smoke from agricultural and plantation residues is expected to increase livestock productivity.
{"title":"Utilization of Biochar and Liquid Smoke to Increase Livestock Performance","authors":"N. Qomariyah, Y. Retnani, A. Jayanegara, E. Wina, I. Permana","doi":"10.14334/wartazoa.v29i4.2077","DOIUrl":"https://doi.org/10.14334/wartazoa.v29i4.2077","url":null,"abstract":"Biochar and liquid smoke may be utilized as feed additives that potentially used as substitutes for antibiotics. These products are derived from the pyrolysis process that utilizes agricultural, plantation and wood residues. This paper aims to review research results regarding the use of biochar and liquid smoke as feed additives in both non-ruminants and ruminants. Information on the use of of biochar and liquid smoke in livestock are available such as the use of 0.5-1 g bamboo charcoal/ kg goat feed increased growth; addition of 0.2-0.6% corn cobs char to chicken feed showed significant increase in body weight; the addition of 0.6% biochar/ kg feed to local cattle feed increased body weight; the use of activated charcoal containing wood vinegar liquid can reduce cryptosporidiosis in goats and cattle. The opportunity to use agricultural and plantation residues as raw materials for generating biochar and liquid smoke is one of the breakthroughs in realizing the concept of sustainable and environmentally friendly of bioindustrial agriculture. The use of biochar and liquid smoke from agricultural and plantation residues is expected to increase livestock productivity.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"29 1","pages":"171-182"},"PeriodicalIF":0.3,"publicationDate":"2019-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47331046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-07DOI: 10.14334/wartazoa.v29i4.2009
Mohammad Ikhsan Shiddieqy, N. Pratiwi, B. Soewandi
Cattle is one of the commodities that have a national priority to achieve self-sufficiency in animal protein food for the Indonesian people. Beside beef sufficiency, demand on good quality meat in Indonesia is also increasing. Good quality carcass with lower fat content can give higher economic value. The purpose of this paper is to discuss the use of molecular markers to improve the quality of beef cattle carcass. Heritability value in carcass characteristic is low to moderate value, but it can be increased by selection using certain molecular markers. Several genes have been identified and correlated with carcass quality in several breed of cattle. Study in Bali cattle showed that the Calpain (CAPN1) and Calpastatin (CASTN) genes could be used as molecular markers to select Bali cattle with higher quality carcass because these genes were associated with carcass quality traits. In conclusion that Calpain (CAPN1) and Calpastatin (CASTN) genes can be used for local beef cattle selection toward better carcass quality and have opportunity to be utilized to improve carcass quality of other beef cattle breeds in Indonesia.
{"title":"Utilization of Molecular Marker to Improve Cattle Carcass Quality in Indonesia","authors":"Mohammad Ikhsan Shiddieqy, N. Pratiwi, B. Soewandi","doi":"10.14334/wartazoa.v29i4.2009","DOIUrl":"https://doi.org/10.14334/wartazoa.v29i4.2009","url":null,"abstract":"Cattle is one of the commodities that have a national priority to achieve self-sufficiency in animal protein food for the Indonesian people. Beside beef sufficiency, demand on good quality meat in Indonesia is also increasing. Good quality carcass with lower fat content can give higher economic value. The purpose of this paper is to discuss the use of molecular markers to improve the quality of beef cattle carcass. Heritability value in carcass characteristic is low to moderate value, but it can be increased by selection using certain molecular markers. Several genes have been identified and correlated with carcass quality in several breed of cattle. Study in Bali cattle showed that the Calpain (CAPN1) and Calpastatin (CASTN) genes could be used as molecular markers to select Bali cattle with higher quality carcass because these genes were associated with carcass quality traits. In conclusion that Calpain (CAPN1) and Calpastatin (CASTN) genes can be used for local beef cattle selection toward better carcass quality and have opportunity to be utilized to improve carcass quality of other beef cattle breeds in Indonesia.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"29 1","pages":"193-204"},"PeriodicalIF":0.3,"publicationDate":"2019-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48254480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-07DOI: 10.14334/wartazoa.v29i4.2018
F. Rachmawati, H. H. S. Purba, S. M. Noor
Mycoplasma bovis is one of the pathogens, causes respiratory, reproductive, mastitis and arthritis disorders in cattle. Due to the economic impact of this disease, Mycoplasma bovis must be free on farms. There are no pathognomonic symptoms of Mycoplasma infection, so it needs laboratory confirmation to diagnose. Many countries have routinely examined M. bovis on cases of mastitis, arthritis, pneumonia and reproductive disorders. There was no cases of respiratory disorder in cattle related to M. bovis infection reported in Indonesia. The fact that, in many countries almost that cases related to the presence of M. bovis. The presence of M. bovis in Indonesia should be investigated, considering that Indonesia imports cattle from Australia and New Zealand that expose to M. bovis. This paper discusses the incidence of M. bovis infection in many countries including its economic impact, clinical symptoms, and method of diagnosis and control of disease to anticipate the emergence of this disease in Indonesia.
{"title":"Anticipation of Emerging Disease Mycoplasma bovis: Control and Countermeasures","authors":"F. Rachmawati, H. H. S. Purba, S. M. Noor","doi":"10.14334/wartazoa.v29i4.2018","DOIUrl":"https://doi.org/10.14334/wartazoa.v29i4.2018","url":null,"abstract":"Mycoplasma bovis is one of the pathogens, causes respiratory, reproductive, mastitis and arthritis disorders in cattle. Due to the economic impact of this disease, Mycoplasma bovis must be free on farms. There are no pathognomonic symptoms of Mycoplasma infection, so it needs laboratory confirmation to diagnose. Many countries have routinely examined M. bovis on cases of mastitis, arthritis, pneumonia and reproductive disorders. There was no cases of respiratory disorder in cattle related to M. bovis infection reported in Indonesia. The fact that, in many countries almost that cases related to the presence of M. bovis. The presence of M. bovis in Indonesia should be investigated, considering that Indonesia imports cattle from Australia and New Zealand that expose to M. bovis. This paper discusses the incidence of M. bovis infection in many countries including its economic impact, clinical symptoms, and method of diagnosis and control of disease to anticipate the emergence of this disease in Indonesia.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"29 1","pages":"205-211"},"PeriodicalIF":0.3,"publicationDate":"2019-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45460772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-04DOI: 10.14334/wartazoa.v29i2.1951
D. Hewajuli, N. Dharmayanti
Vaccination and antiviral drug are often used to control influenza. However, the effectiveness of vaccine was impaired due to the emergence of new variant of virus strain. Antiviral drug consists of prophylactic and curative substances, namely M2 ion channel inhibitors (adamantane; amantadine and rimantadine) and neuraminidase (NA) inhibitors (NAIs; oseltamivir, zanamivir, peramivir, laninamivir). The synthesis and modification of antiviral neuraminidase (NA) inhibitors (NAIs) and adamantanes increased the antiviral effectiveness. The mechanism of the neuraminidase inhibitor is to prevent influenza infection by inhibiting the release of the virus from internal cells. Adamantane is antiviral drug that selectively inhibits the flow of H+ ions through M2 protein to prevent the uncoating virus particles getting into the endosome. The substitution of (H275Y, S247N, I223L, K150N, R292K, I222T, R152K, R118K, E119V) on NA protein caused resistance of avian influenza virus against the neuraminidase inhibitor. The combination of mutations (S247N, I223L, K150N) increased the resistance of influenza A (H5N1) virus. The diffusion of adamantane resistance varies among HA subtypes, the species of host, the period of isolation, and region. Mutations at residues of 26, 27, 30, 31 or 34 transmembrane M2 protein caused adamantane resistance. The unique substitution (V27I) of M2 protein of clade 2.3.2 H5N1 subtype isolated in Indonesia in 2016 has been contributed to the amantadine resistance. Antiviral combination of M2 ion channel inhibitors and neuraminidase (NA) inhibitors is effective treatments for the resistance.
{"title":"Efficacy, Mechanism and Antiviral Resistance of Neuraminidase Inhibitors and Adamantane against Avian Influenza","authors":"D. Hewajuli, N. Dharmayanti","doi":"10.14334/wartazoa.v29i2.1951","DOIUrl":"https://doi.org/10.14334/wartazoa.v29i2.1951","url":null,"abstract":"Vaccination and antiviral drug are often used to control influenza. However, the effectiveness of vaccine was impaired due to the emergence of new variant of virus strain. Antiviral drug consists of prophylactic and curative substances, namely M2 ion channel inhibitors (adamantane; amantadine and rimantadine) and neuraminidase (NA) inhibitors (NAIs; oseltamivir, zanamivir, peramivir, laninamivir). The synthesis and modification of antiviral neuraminidase (NA) inhibitors (NAIs) and adamantanes increased the antiviral effectiveness. The mechanism of the neuraminidase inhibitor is to prevent influenza infection by inhibiting the release of the virus from internal cells. Adamantane is antiviral drug that selectively inhibits the flow of H+ ions through M2 protein to prevent the uncoating virus particles getting into the endosome. The substitution of (H275Y, S247N, I223L, K150N, R292K, I222T, R152K, R118K, E119V) on NA protein caused resistance of avian influenza virus against the neuraminidase inhibitor. The combination of mutations (S247N, I223L, K150N) increased the resistance of influenza A (H5N1) virus. The diffusion of adamantane resistance varies among HA subtypes, the species of host, the period of isolation, and region. Mutations at residues of 26, 27, 30, 31 or 34 transmembrane M2 protein caused adamantane resistance. The unique substitution (V27I) of M2 protein of clade 2.3.2 H5N1 subtype isolated in Indonesia in 2016 has been contributed to the amantadine resistance. Antiviral combination of M2 ion channel inhibitors and neuraminidase (NA) inhibitors is effective treatments for the resistance.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"29 1","pages":"61-74"},"PeriodicalIF":0.3,"publicationDate":"2019-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49658955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-04DOI: 10.14334/wartazoa.v29i2.1890
S. A. Asmarasari, C. Sumantri, A. Gunawan, E. Taufik, A. Anggraeni
The objective of this paper is to explore some efforts to increase milk protein of dairy cows through a milk protein control gene selection approach. Improving the quality of cow's milk has shifted to increase milk protein levels, due to nutritional and economic interest. The breeding process in producing dairy cattle with the advantage of having high milk protein content is more effectively carried out by molecular biotechnology approaches. The content of cow's milk protein is controlled by cow's milk protein control genes. In the process to produce dairy cattle with the advantage of having high milk protein content, it can be done with a selection approach based on milk protein control genes, namely CSN1S1, CSN2, CSN1S2, and CSN3. Once known, the main controller gene that causes high levels of cow's milk protein, it will be easy to identify dairy cattle that have these advantages, so that the selection of dairy cattle can be done since at early age.
{"title":"Candidate Gene of Milk Protein for Genetic Improvement of Dairy Cattle","authors":"S. A. Asmarasari, C. Sumantri, A. Gunawan, E. Taufik, A. Anggraeni","doi":"10.14334/wartazoa.v29i2.1890","DOIUrl":"https://doi.org/10.14334/wartazoa.v29i2.1890","url":null,"abstract":"The objective of this paper is to explore some efforts to increase milk protein of dairy cows through a milk protein control gene selection approach. Improving the quality of cow's milk has shifted to increase milk protein levels, due to nutritional and economic interest. The breeding process in producing dairy cattle with the advantage of having high milk protein content is more effectively carried out by molecular biotechnology approaches. The content of cow's milk protein is controlled by cow's milk protein control genes. In the process to produce dairy cattle with the advantage of having high milk protein content, it can be done with a selection approach based on milk protein control genes, namely CSN1S1, CSN2, CSN1S2, and CSN3. Once known, the main controller gene that causes high levels of cow's milk protein, it will be easy to identify dairy cattle that have these advantages, so that the selection of dairy cattle can be done since at early age.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"29 1","pages":"97-107"},"PeriodicalIF":0.3,"publicationDate":"2019-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47673051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-04DOI: 10.14334/wartazoa.v29i2.1955
P. W. Prihandini, D. Maharani
Genetic improvement for economic traits such as growth is important in beef cattle selection program. This paper discusses melanocortin-4 receptor (MC4R) gene as a candidate gene for rapid growth based on its function, location and pathway, as well as its polymorphism and identified genotypes associated with economic traits, and its application as a marker-assisted selection. MC4R is a prominent gene encoding growth traits and has a critical role in mediating the effect of leptin in regulating food consumption and energy balance. Many identified MC4R gene polymorphisms are associated with growth traits. The MC4R gene, therefore, is considered as a functional candidate gene for growth traits and can be used as a marker in selection program based on molecular genetics. The use of molecular markers such as MC4R gene, therefore, can be applied in genetic improvement program for growth traits in cattle. By using MC4R gene, the efforts to build breeding system in small populations can be proposed. For instance, with an effective population size (Ne) of about 40 heads, the first generation (G1) will be obtained with a composition of GG 61.1%, CG 33.3% and CC 5.6% and male and female ratio of 178:11 and generates ideal Ne of 41 heads. In the third generation, therefore, GG composition will be 100%. The results of molecular analysis can be further used as a guideline in the development and genetic improvement strategies of beef cattle.
{"title":"Melanocortin-4 Receptor (MC4R) Gene as the Main Gene for Rapid Growth Selection in Beef Cattle","authors":"P. W. Prihandini, D. Maharani","doi":"10.14334/wartazoa.v29i2.1955","DOIUrl":"https://doi.org/10.14334/wartazoa.v29i2.1955","url":null,"abstract":"Genetic improvement for economic traits such as growth is important in beef cattle selection program. This paper discusses melanocortin-4 receptor (MC4R) gene as a candidate gene for rapid growth based on its function, location and pathway, as well as its polymorphism and identified genotypes associated with economic traits, and its application as a marker-assisted selection. MC4R is a prominent gene encoding growth traits and has a critical role in mediating the effect of leptin in regulating food consumption and energy balance. Many identified MC4R gene polymorphisms are associated with growth traits. The MC4R gene, therefore, is considered as a functional candidate gene for growth traits and can be used as a marker in selection program based on molecular genetics. The use of molecular markers such as MC4R gene, therefore, can be applied in genetic improvement program for growth traits in cattle. By using MC4R gene, the efforts to build breeding system in small populations can be proposed. For instance, with an effective population size (Ne) of about 40 heads, the first generation (G1) will be obtained with a composition of GG 61.1%, CG 33.3% and CC 5.6% and male and female ratio of 178:11 and generates ideal Ne of 41 heads. In the third generation, therefore, GG composition will be 100%. The results of molecular analysis can be further used as a guideline in the development and genetic improvement strategies of beef cattle.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"29 1","pages":"85-96"},"PeriodicalIF":0.3,"publicationDate":"2019-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45230432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-04DOI: 10.14334/wartazoa.v29i2.1999
Hasim Munawar, K. Karim, S. Piletsky
Fumonisin contamination in food is limited around 2 – 4 ppm and in feed for different animals varies from 5 to 100 ppm. This regulation is to prevent animal and human from carcinogenic effect from fumonisins. Measurement of fumonisins frequently uses chromatography methods such as High-Performance Liquid Chromatography (HPLC) and Liquid chromatography tandem-mass spectrometry (LCMS/MS); however, the sample preparation and analysis process for these methods are costly and time consuming. Immunoassays have also been employed for detecting fumonisins in food or feed. Unfortunately, the instability of antibody to harsh condition such as high temperature and pH becomes the drawback for immunoassay method. Currently, the technology based on molecularly imprinting, which is called synthetic antibody, has been established for replacing antibody functions. Therefore, the aim of this review is to describe development of molecularly imprinted polymer (MIP) in fumonisin analysis in feed and food. Herein, the composition and production of MIP were described comprehensively. Bulk polymerization and solid phase synthesis were methods for production of MIP in micro and nano sizes. The application of MIP was reported for sample preparation as solid phase extraction measured continuously by HPLC showing the high recovery (> 60%). Then, MIP replaced antibody in direct competitive enzyme-linked immunosorbent assay (ELISA) for quantifying fumonisins in maize with high recovery (>90%) and limit detection (2 – 6 pM). Lastly, MIP was also employed in electrochemical sensor application as receptor for recognizing fumonisin in milk and maize. In conclusion, the performance of MIP has been applied successfully for fumonisin analysis comprehensively from sample preparation and quantification. The MIP would be developed for wider application for other toxins in feed or food such as veterinary drug, heavy metals, or pesticides.
{"title":"Utilization of Synthetic Antibody for Fumonisin Determination in Feed and Food","authors":"Hasim Munawar, K. Karim, S. Piletsky","doi":"10.14334/wartazoa.v29i2.1999","DOIUrl":"https://doi.org/10.14334/wartazoa.v29i2.1999","url":null,"abstract":"Fumonisin contamination in food is limited around 2 – 4 ppm and in feed for different animals varies from 5 to 100 ppm. This regulation is to prevent animal and human from carcinogenic effect from fumonisins. Measurement of fumonisins frequently uses chromatography methods such as High-Performance Liquid Chromatography (HPLC) and Liquid chromatography tandem-mass spectrometry (LCMS/MS); however, the sample preparation and analysis process for these methods are costly and time consuming. Immunoassays have also been employed for detecting fumonisins in food or feed. Unfortunately, the instability of antibody to harsh condition such as high temperature and pH becomes the drawback for immunoassay method. Currently, the technology based on molecularly imprinting, which is called synthetic antibody, has been established for replacing antibody functions. Therefore, the aim of this review is to describe development of molecularly imprinted polymer (MIP) in fumonisin analysis in feed and food. Herein, the composition and production of MIP were described comprehensively. Bulk polymerization and solid phase synthesis were methods for production of MIP in micro and nano sizes. The application of MIP was reported for sample preparation as solid phase extraction measured continuously by HPLC showing the high recovery (> 60%). Then, MIP replaced antibody in direct competitive enzyme-linked immunosorbent assay (ELISA) for quantifying fumonisins in maize with high recovery (>90%) and limit detection (2 – 6 pM). Lastly, MIP was also employed in electrochemical sensor application as receptor for recognizing fumonisin in milk and maize. In conclusion, the performance of MIP has been applied successfully for fumonisin analysis comprehensively from sample preparation and quantification. The MIP would be developed for wider application for other toxins in feed or food such as veterinary drug, heavy metals, or pesticides.","PeriodicalId":42818,"journal":{"name":"Wartazoa-Buletin Ilmu Peternakan dan Kesehatan Hewan Indonesia","volume":"29 1","pages":"51-60"},"PeriodicalIF":0.3,"publicationDate":"2019-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48069036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-04DOI: 10.14334/wartazoa.v29i2.2015
P. Widiyanti, M. Sudarwanto, E. Sudarnika, R. Widiastuti
The livestock sector can improve the community's economy and has a role in fulfilling food needs, especially animal protein. One of the problems in the livestock sector is the presence of infectious diseases that consequently need treatment using veterinary drugs. This paper describes the use of enrofloxacin antibiotics as veterinary drug and their residual hazards on public health. Enrofloxacin is an antibiotic from the family of fluoroquinolones (second generation of quinolone). Enrofloxacin is a broad-spectrum antibiotic that is effective to kill Gram positive and negative bacteria, so it was used for the treatment of various diseases in animals. Pharmacokinetically, enrofloxacin will be metabolized into ciprofloxacin and other metabolites. The improper use of enrofloxacin antibiotics caused residues in food products of animal origin, microbial resistance and toxicity, therefore the use of enrofloxacin needs to be monitored and evaluated for the sake of animal health and society.
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