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POTENTIAL OF ENDOPHY BACTERIAL ISOLATE FROM LEAF OF CHINESE TEMPERATURE (Cassia alata L) ON THE GROWTH OF THE BACTERIA OF STAPHYLOCOCCUS AUREUS 中国温决明子叶片内生细菌分离物对金黄色葡萄球菌生长的潜力
Pub Date : 2022-03-21 DOI: 10.47918/jhts.v3i1.332
Enisa Kobandaha, Syam s kumaji, Adnan Malaha
ABSTRACT    This research aims to To determine the effect of endophytic bacterial isolates from the leaves of the Chinese Ketepeng plant (Cassia Alata L) on the growth of Staphylococcus aureus and to determine the significant difference between each treatment of endophytic bacterial isolates from the leaves of the Ketepeng Cina (Cassia Alata L) plant against Staphylococcus aureus. This research method used a completely randomized design (CRD) with 4 treatments and 6 replications. The treatments were: BEKC 1 (Chinese Ketepeng Endophytic Bacteria 1), BEKC 2 (Chinese Ketepeng Endophytic Bacteria 2), Positive Control (Chloramphenicol), and Negative Control (Aquades). The results obtained two isolates of endophytic bacteria, namely isolates BEKC 1 with an inhibition zone of 9mm included in the medium category, and isolates BEKC 2 with an inhibition zone of 10mm included in the strong category. Analysis resultthat there was an effect of endophytic bacterial isolates from the leaves of the Chinese ketepeng plant (Cassia alata L) on the growth of Staphylococcus aureus bacteria. Then from the results of further tests using Duncan's test, it was found that each treatment, namely the negative control treatment was significantly different from BEKC 1, BEKC 2 and positive control, while there was no significant difference between BEKC 1, BEKC 2 and positive control. Keywords : Endophytic Bacteria, Chinese Ketepeng (Cassia alata L), Staphylococcus aureus
摘要本研究旨在确定中国克特蓬(决明子Alata L)叶片内生细菌分离物对金黄色葡萄球菌生长的影响,并确定克特蓬(决明子Alata L)叶片内生细菌分离物在不同处理下对金黄色葡萄球菌的抑制作用的显著性差异。本研究采用完全随机设计(CRD), 4个处理,6个重复。处理分别为:bekc1(中国克特蓬内生细菌1号)、bekc1(中国克特蓬内生细菌2号)、阳性对照(氯霉素)和阴性对照(Aquades)。结果得到2株内生细菌,bekc1菌株抑菌区为9mm,属于中等抑菌区,bekc2菌株抑菌区为10mm,属于强抑菌区。分析结果表明,克特蓬叶片内生细菌分离物对金黄色葡萄球菌的生长有影响。然后用Duncan’s检验法进一步检验结果发现,各处理,即阴性对照处理与bekc1、bekc2和阳性对照存在显著差异,而bekc1、bekc2和阳性对照之间无显著差异。关键词:内生细菌,中国克特蓬,金黄色葡萄球菌
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引用次数: 0
IDENTIFICATION OF BORAX AND FORMALIN CONTENT IN WET NOODLES AND MEATBALLS IN GORONTALO CITY 哥伦塔洛市湿面和肉丸中硼砂和福尔马林含量的测定
Pub Date : 2022-03-21 DOI: 10.47918/jhts.v3i1.270
Della Fatimi Modeong, Farid Buhari, Ni Luh Arwati
This study aims to determine the presence of additives in food samples in wet noodles and meatballs. The research method used is an analytical method with an experimental design (pre-experimental, the object of research is wet noodles and meatballs. Then both samples are added with formalin and borax to determine whether the two samples contain borax and formalin or not. The results showed that none of the samples contained borax. If a food contains borax, then when the food is dripped with borax (indicator) it will experience a color change to brick red. However, during the formalin test, some of these foods contain formalin, namely, wet noodles, and meatballs, where this is known from the changing color, namely when the two samples are dripped with CuSO4 there is a change in color, namely a yellowish color which indicates that the sample contains formalin.
本研究旨在确定湿面和肉丸食品样品中添加剂的存在。使用的研究方法是具有实验设计的分析方法(预实验),研究对象是湿面和肉丸。然后将两个样品分别加入福尔马林和硼砂,以确定两个样品是否含有硼砂和福尔马林。结果表明,样品中不含硼砂。如果食物中含有硼砂,那么当食物滴上硼砂(指示剂)时,食物的颜色就会变成砖红色。然而,在福尔马林测试过程中,这些食品中有一些含有福尔马林,即湿面条和肉丸,这可以从颜色的变化中得知,即当两个样品滴入CuSO4时,颜色发生变化,即黄色,这表明样品含有福尔马林。
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引用次数: 0
IDENTIFICATION OF AMYLUM CHARACTERISTICS USING MICROSCOPIC 淀粉特性的显微鉴定
Pub Date : 2022-03-21 DOI: 10.47918/jhts.v2i4.251
Findriyani Paus, Hasrul Abdullah, Agusrianto Yusuf
Starch is stored in permanent food reserves for plants, in seeds, core fingers, bark, roots of perennials, and tubers. Starch is 50-65% dry weight of wheat seeds and 80% dry matter of potato tubers. Starch is a type of polysaccharide that is widely found in nature, namely most plants are found in tubers, leaves, stems, and seeds.The purpose of this study was to identify the different types of starch, especially in potato, cassava, and rice. The method used in this research is the experimental method. The experimental method is an experiment conducted to prove a hypothesis by using potatoes, sweet potatoes and rice as samples for starch observations. The results showed that the modified potato starch (Solanum tuberosum) was white in color, curd, in the form of fine grains, odorless and tasteless. Microscopic examination showed the hilus was a point at a narrow end with clearly visible lamellae, a single starch arrangement. The characteristics of modified starch manihot utilissima are white ivory, in the form of fine grains, odorless and tasteless. Microscopic examination showed that the hilus is located in the middle, the lamellae are not clear, the arrangement of starch is single. The characteristics of rice starch (Oryza sativa) are ivory white, in the form of fine grains, odorless and tasteless. In the microscopic test showedhilum in the middle not clearly visible, no lamellae, single starch arrangement.
淀粉储存在植物的永久食物储备中,如种子、茎核、树皮、多年生植物的根和块茎。淀粉占小麦种子干重的50-65%,马铃薯块茎干重的80%。淀粉是自然界中广泛存在的一种多糖,即大多数植物的块茎、叶、茎和种子中都含有淀粉。这项研究的目的是鉴定不同类型的淀粉,特别是在马铃薯、木薯和大米中。本研究采用的方法是实验法。实验法是以土豆、红薯和大米为样本进行淀粉观察的一种验证假设的实验。结果表明,改性马铃薯淀粉呈白色凝乳状,颗粒细,无臭、无味。显微镜检查显示,门是一个点在一个狭窄的末端,有清晰可见的薄片,单一淀粉排列。改性淀粉的特点是乳白色,颗粒细,无臭无味。镜检显示:裂口位于中部,片层不清楚,淀粉排列单一。大米淀粉(Oryza sativa)的特点是乳白色,呈细粒状,无味。镜检显示,芽门位于中部不明显,无片层,淀粉排列单一。
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引用次数: 0
DESCRIPTION OF THE RHEUMATOID FACTOR TEST IN WOMEN THE MENOPAUSE TO DETECT RHEUMATOID ARTHRITIS IN ULAPATO-A VILLAGE, GORONTALO DISTRICT 戈伦塔洛地区乌拉帕托-阿村绝经期妇女类风湿因子试验检测类风湿关节炎的研究
Pub Date : 2022-03-20 DOI: 10.47918/jhts.v2i3.199
Pratiwi Dunggio, Titin Dunggio, Rita Amini Warastuti
         Based on data from the Gorontalo provincial health office in 2017 the incidence of Rheumatoid Arthritis reached 14,391 the number of cases. Rheumatoid Arthritis is a chronic autoimmune inflammatory system disease whose cause is unknown. Symptoms of Rheumatoid Arthritis include experiencing stiffness in the joints in the morning, and swelling of the joints. This study aims to determine the results of Rheumatoid Arthritis test in menopausal women in Ulapato-A Village, Gorontalo District by examining Rheumatoid Factors.        This research is a quantitative descriptive study with a total of 34 samples. Using manual Rheumatoid Factor Check. This research was processed through the IBM SPSS Statistics V25.0 program. then presented in tabular form and reported as a percentage using the Frequency Distribution formula.        From the results obtained, 8 samples (23.5%) were positive while 26 samples (76.5%) were negative. In conclusion Rheumatoid factor in menopausal women is mostly negative. It is hoped that Rheumatoid Arthritis sufferers maintain their health by living a healthy lifestyle and doing light exercise and for other researchers to also carry out semi-quantitative follow-up examinations if they get a positive sample.    
根据戈龙塔洛省卫生办公室2017年的数据,类风湿关节炎的发病率达到14,391例。类风湿性关节炎是一种慢性自身免疫性炎症系统疾病,其病因尚不清楚。类风湿关节炎的症状包括早晨关节僵硬和关节肿胀。本研究旨在通过检测类风湿因子来确定Gorontalo区Ulapato-A村绝经期妇女类风湿关节炎试验的结果。本研究为定量描述性研究,共34个样本。使用手动类风湿因子检查。本研究通过IBM SPSS Statistics V25.0程序进行处理。然后以表格形式呈现,并使用频率分布公式以百分比形式报告。结果显示,阳性8份(23.5%),阴性26份(76.5%)。结论:绝经期妇女类风湿因子多为阴性。希望类风湿关节炎患者通过健康的生活方式和轻度运动来保持健康,并希望其他研究人员在样本呈阳性的情况下也能进行半定量的随访检查。
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引用次数: 0
Test the Effectiveness of Bay Leaf Extract (Syzygium Polyanthum) in Lowering Uric Acid Levels in Male White Rats (Rattus Novergicus) 月桂叶提取物降低雄性白鼠尿酸水平的试验
Pub Date : 2022-03-20 DOI: 10.47918/jhts.v2i3.276
Lila Muliani, Gita Puspita, Rifka Anggraini Anggai
This study aims to determine whether there is a therapeutic effect on bay leaf extract (Syzygium polyanthum) in reducing uric acid levels in male white rats (Rattus novergicus) and to determine what dose of bay leaf extract (Syzyzgium polyanthum) is effective in reducing uric acid levels in male white rat (Rattus novergicus). The method in this study used Laboratory Experiments with Pretest-Posttest Control Group Design, the treatment groups were group I (negative control), group II (positive control), group III (bay leaf extract 0.4581 g), group IV (extract bay leaf 0.8181 g), and group V (bay leaf extract 1.1781 g). The results showed that bay leaf extract (Syzygium polyhantum) was positive for flavonoid compounds. Statistical data analysis by Kruskal Walis showed that 0.003 p significance value 0.005 means that there is a significant difference between uric acid levels before administration of bay leaf extract and uric acid levels after administration of bay leaf extract and continued with Duncan's analysis to see differences in effective doses.
本研究旨在确定月桂叶提取物(合欢蓼)是否具有降低雄性白鼠(褐家鼠)尿酸水平的治疗作用,并确定多大剂量的月桂叶提取物(合欢蓼)能有效降低雄性白鼠(褐家鼠)尿酸水平。本研究方法采用前-后对照设计的室内实验,处理组为ⅰ组(阴性对照)、ⅱ组(阳性对照)、ⅲ组(月桂叶提取物0.4581 g)、ⅳ组(月桂叶提取物0.8181 g)、ⅴ组(月桂叶提取物1.1781 g)。结果表明,月桂叶提取物黄酮类化合物阳性。Kruskal Walis的统计数据分析显示,0.003 p显著值0.005表示服用月桂叶提取物前的尿酸水平与服用月桂叶提取物后的尿酸水平存在显著差异,并继续进行Duncan的分析,观察有效剂量的差异。
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引用次数: 1
Evaluation of Patient Satisfaction BPJS Outboard with Pharmaceutical Services in Pharmaceutical Installations RSUD Dr. M.M Dunda Limboto BPJS外部对制药设施中药学服务的患者满意度评估
Pub Date : 2022-03-20 DOI: 10.47918/jhts.v2i2.275
A. Mustapa, Rini Daud Supu, Serly Daud
This study aims to find out the level of satisfaction of outpatient BPJS patients to pharmaceutical services in the pharmaceutical installation of Dr. M.M DundaLimboto Hospital which is seen based on five dimensions, namely reliability dimension, responsiveness dimension, assurance dimension, tangible dimension, and empathy dimension. The type of this research is descriptive observational quantitative with a cross sectional research approach. The sources of data in this study are BPJS Rajal patients who receive pharmaceutical services at the Pharmacy Installation of RSUDdDr. M.M, DundaLimboto, with data obtained via filling out a questionnaire for each respondent, using five assessment scores, very satisfied 5, satisfied 4, quite satisfied 3, dissatisfied 2, and dissatisfied 1. The method used in the current studywas the customer satisfaction index (CSI) method aiming at knowing the level of customer satisfaction. The results show that the satisfaction of outpatient BPJS patients to pharmaceutical services at the pharmaceutical installation of Dr. M.M DundaLimboto Hospital is 79.93% categorized as satisfied.
本研究旨在了解M.M DundaLimboto医生医院药房门诊BPJS患者对药学服务的满意度,该满意度基于五个维度,即可靠性维度、响应性维度、保证维度、有形维度和共情维度。本研究的类型是描述性观察定量与横断面研究方法。本研究的数据来源是在rsuddr . Pharmacy Installation接受药学服务的BPJS Rajal患者。M.M, DundaLimboto,通过填写每位受访者的问卷获得数据,使用五个评估分数,非常满意5分,满意4分,相当满意3分,不满意2分,不满意1分。本研究采用的方法是顾客满意指数(CSI)法,旨在了解顾客满意程度。结果显示,门诊BPJS患者对Dr. M.M DundaLimboto医院药房的药学服务满意度为79.93%,为满意;
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引用次数: 0
DIFFERENCES IN EXAMINATION OF USUS NEMATODE EGGS ON FESES BETWEEN FLOTATION METHODS AND SEDIMENTATION METHODS FOR STUDENTS AT SDN 11 TELAGA 在SDN 11 telaga学生中,浮法和沉淀法对泥上线虫卵检测的差异
Pub Date : 2022-03-20 DOI: 10.47918/jhts.v2i2.154
Dian Putri Muhrija, Agusriyanto Yusuf, Natan Pasiga
Various types of intestinal worms are still a public health problem and are often found both in cities and in villages in Indonesia, which can lead to anemia, malnutrition, impaired growth and impaired intelligence. This also leads to choosing the right method to determine a person's worm status. The use of a fecal examination method that has a high level of sensitivity and specificity is very important in order to obtain a worm status or accurate results. A person's worms status can be ascertained by finding worm eggs in a fecal laboratory examination. This study aims to determine differences in the results of examining intestinal nematode eggs in feces between Flotation methods and sedimentation methods for students at SDN 11 Telaga. This research is a quantitative analytic comparative method, with a sample size of 35 people from a population of 54 people. The data analysis used is univariate analysis and bivariate analysis. The results were tested with Wilcoxon. The results of this study showed that the flotation method was positive, and in the sedimentation method there were no intestinal nematode eggs.
各种肠道蠕虫仍然是一个公共卫生问题,在印度尼西亚的城市和农村都经常发现,这可能导致贫血、营养不良、生长受损和智力受损。这也导致选择正确的方法来确定一个人的蠕虫状态。为了获得蠕虫状态或准确的结果,使用具有高灵敏度和特异性的粪便检查方法非常重要。一个人的蠕虫状态可以通过在粪便实验室检查中发现虫卵来确定。本研究旨在确定浮选法和沉淀法对SDN 11 Telaga学生粪便中肠道线虫卵检测结果的差异。本研究采用定量分析比较的方法,从54人的人群中选取35人作为样本。使用的数据分析是单变量分析和双变量分析。用Wilcoxon对结果进行检验。本研究结果表明,浮选法为阳性,沉淀法未检出肠道线虫卵。
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引用次数: 0
Evaluation of the Use of Antibiotics Using the ATC/DDD Method in Post-Surgery Patients at the Inpatient Installation of dr. Hospital. Zainal Umar Sidiki 医院医生住院部术后患者ATC/DDD法抗生素使用评价Zainal Umar Sidiki
Pub Date : 2022-03-20 DOI: 10.47918/jhts.v2i3.280
Noviyanti I. Panu, Sarini Pani, Rini Daud Supu
In general, the use of antibiotics in health care facilities is more widely used in hospitals, especially in post-surgical patients. Infections that often appear in post-surgical patients are surgical wound infections. Surgical wound infection (ILO) is an infection in the incision area or organ-space that occurs in patients after surgery. Antibiotics are the most widely used drug class in the world due to the high incidence of bacterial infections. One of the most common is resistance to antibiotics. The purpose of this research To determine the use of antibiotics in post-surgical patients in the inpatient installation of RSUD dr. Zainal Umar Sidiki. Evaluation the use of antibiotics was assessed using the Defined Daily Dose (DDD)/100 bed-days and 90% DU segment. The results of the evaluation showed that the most widely used antibiotic was ceftriaxone with a value of 27.54 DDD/100 bed-days and quantitative use of antibiotics in post-surgical patients at RSUD dr. Zainal Umar Sidki is 69.77 DDD/100 bed-days and antibiotics that fall into the 90% DU segment are ceftriaxone, cefadroxil, cefixime and ciprofloxacin. While those included in the 10% DU segment were clindamycin, moxifloxacin hcl, cefotaxime, amoxicillin and azythromicin.
一般来说,卫生保健设施中抗生素的使用在医院更为广泛,特别是在手术后患者中。术后患者经常出现的感染是手术伤口感染。手术伤口感染(ILO)是指手术后患者在切口区域或器官间隙发生的感染。由于细菌感染的高发病率,抗生素是世界上使用最广泛的药物类别。其中最常见的是对抗生素的耐药性。本研究的目的是确定住院安装RSUD的Zainal Umar Sidiki医生术后患者抗生素的使用情况。抗生素使用评估采用限定日剂量(DDD)/100床日和90% DU段进行评估。评价结果显示,使用最广泛的抗生素为头孢曲松,用量为27.54 DDD/100床日,RSUD Zainal Umar Sidki医生术后患者抗生素定量用量为69.77 DDD/100床日,90% DU段的抗生素为头孢曲松、头孢羟肟、头孢克肟和环丙沙星。而10% DU段包括克林霉素、盐酸莫西沙星、头孢噻肟、阿莫西林和阿奇霉素。
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引用次数: 0
Formulating Cream of Dumbo Catfish Skin Extract (Clarias Gariepinus) for Acceleration of Slice Wound Healing in White Male Rats (Rattus Novergicus) Wistar Straight 小飞象鲶鱼皮提取物(Clarias Gariepinus)加速白色雄性大鼠(Rattus Novergicus) Wistar Straight切片伤口愈合乳膏的研制
Pub Date : 2022-03-20 DOI: 10.47918/jhts.v2i3.279
Nurjannah Mopangga, Muh. Aprianto Paneo, Srimuliani Arbie
  Wound is a condition in which the skin tissue is damaged and some of the skin tissue is lost. Catfish skin is one part that contains collagen.One of the ingredients that has been widely used to maintain a moist atmosphere in wounds is collagen This study aimed to determine the effect of African catfish skin extract cream to determine the effectiveness of collagen from catfish skin extract in accelerating the healing process of cuts in male white rats. The method in this research is experimental laboratory using one sample t-test test data analysis to see the effect of the catfish skin extract cream formula. The research design used was a post test only control group. The experimental group was treated by giving the catfish skin extract cream formula, while the negative control group was not given any treatment. Each cream formula has three concentrations, namely 5%, 7.5% and 12.5% ​​and cream base as a negative control. The results showed that the cream formulation with a concentration of 12.5% ​​had an effect on wound healing on the seventh day, this was seen from physical observation by looking at the changes in the length of the wound which was reduced and the wound had dried. African catfish skin cream has an effect on wound healing with a healing process within 7 days The effectiveness of African catfish skin cream at a concentration of 12.5% ​​has a faster effect on wound healing compared to the 7.5% and 5% formulas
伤口是一种皮肤组织受损和部分皮肤组织丢失的情况。鲶鱼的皮肤是含有胶原蛋白的一部分。胶原蛋白是被广泛用于维持伤口湿润气氛的成分之一。本研究旨在确定非洲鲶鱼皮提取物乳霜的效果,以确定鲶鱼皮提取物胶原蛋白在加速雄性白鼠伤口愈合过程中的有效性。本研究的方法是实验室实验,采用单样本t检验检验数据分析,看鲶鱼皮提取物乳霜配方的效果。本研究采用的设计为单纯的后测对照组。实验组给予鲶鱼皮提取物乳膏配方治疗,阴性对照组不给予任何治疗。每种面霜配方有三种浓度,分别为5%、7.5%和12.5%,以面霜碱为阴性对照。结果表明,浓度为12.5%的乳膏配方对创面愈合的影响在第7天,这是通过观察创面长度的变化,创面减少,创面干燥来观察的。非洲鲶鱼护肤霜具有伤口愈合的效果,在7天内愈合。与7.5%和5%的配方相比,非洲鲶鱼护肤霜浓度为12.5%的效果对伤口愈合的效果更快
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引用次数: 0
FORMULATION AND EVALUATION OF BREAST MILK ETHANOL EXTRACT FRUIT MEAT (Psidium Guajava L.) BY USING VARIATIONS OF EMULGATOR CONCENTRATION 母乳乙醇提取物水果肉(番石榴)的配方及评价通过使用不同的乳化剂浓度
Pub Date : 2022-03-20 DOI: 10.47918/jhts.v2i2.271
Nuryfa D. Buhang, Gita Puspita, Srimuliani Arbie
Preparations cream is a semisolid dosage forms containing one or more ingredients dissolved or dispersed in the base material which one of the plants that is used as a face cream is guava, because it contains vitamin C which is quite high and has the potential as an excellent antioxidant. This study aims to determine the effect of emulsifier concentration on cream preparations and which formulas show stable results. This research is an experimental study with a posttest only design. The population in this study was guava fruit (Psidium guajava L.) from guava trees in Dulomo Selatan Village, Kota Utara District, Gorontalo. The results showed that in formula I (F1) and formula III (F3) there was a rancid odor, for texture and appearance there had been a change from formula I (F1), namely soft and dense texture and formula II (F2) and formula III (F3). to a soft and fluid texture. The result of measuring the pH of cream preparations in formula 1 (F1) is 7, formula (F2) is 7 and formula (F3) is 7, the test for spreadability of cream preparations in Formula 1 (F1) is 5.25 cm, Formula 2 (F2) 4 .5 cm and Formula 3 (F3) 5.25 cm, the centrifugation test for cream preparations in Formula 1 (F1), Formula 2 (F2) and Formula 3 (F3) separated for 2 hours.
面霜是一种半固体剂型含有一种或多种成分溶解或分散在基础材料中,其中一种用作面霜的植物是番石榴,因为它含有很高的维生素C,有潜力成为一种极好的抗氧化剂。本研究旨在确定乳化剂浓度对乳霜制剂的影响,并确定效果稳定的配方。本研究是一项仅后测设计的实验研究。本研究的种群为来自戈伦塔洛市Kota Utara区Dulomo Selatan村番石榴树的番石榴果实(Psidium guajava L.)。结果表明,式I (F1)和式III (F3)中存在腐臭气味,在质地和外观上与式I (F1)相比有了变化,即质地柔软致密,与式II (F2)和式III (F3)相比有了变化。变成柔软流畅的质地。公式1 (F1)中乳膏制剂的pH值测定结果为7,公式(F2)为7,公式(F3)为7,公式1 (F1)中乳膏制剂的涂抹性试验为5.25 cm,公式2 (F2)为4.5 cm,公式3 (F3)为5.25 cm,公式1 (F1)、公式2 (F2)、公式3 (F3)中乳膏制剂的离心试验分离2小时。
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引用次数: 0
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Journal of Health, Technology and Science (JHTS)
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