Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.09
İ. Coşkun Benlidayı
EXERCISING IS ESSENTIAL FOR HEALTHY AGING
锻炼对健康衰老是必不可少的
{"title":"EXERCISING IS ESSENTIAL FOR HEALTHY AGING","authors":"İ. Coşkun Benlidayı","doi":"10.56543/aaeeu.2022.1.2.09","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.09","url":null,"abstract":"EXERCISING IS ESSENTIAL FOR HEALTHY AGING","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125988043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.04
N. Gokcen
The early onset of ovarian aging results in decreased ovarian reserve and premature ovarian insufficiency (POI), leading to infertility and early menopause. POI is associated with systemic autoimmune diseases. The aim of the present narrative review article was to review and summarize the recent literature regarding premature ovarian insufficiency and reduced ovarian reserve in patients with autoimmune diseases, especially SSc. The electronic database search for the review was conducted in Pubmed/Medline. This literature search indicates that POI is quite common in systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Even though limited studies demonstrate that POI is also seen in systemic sclerosis, the underlying mechanism remains unclear. Therefore, further studies are needed to understand the influence of SSc on ovarian homeostasis.
{"title":"PREMATURE OVARIAN INSUFFICIENCY IN SYSTEMIC SCLEROSIS","authors":"N. Gokcen","doi":"10.56543/aaeeu.2022.1.2.04","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.04","url":null,"abstract":"The early onset of ovarian aging results in decreased ovarian reserve and premature ovarian insufficiency (POI), leading to infertility and early menopause. POI is associated with systemic autoimmune diseases. The aim of the present narrative review article was to review and summarize the recent literature regarding premature ovarian insufficiency and reduced ovarian reserve in patients with autoimmune diseases, especially SSc. The electronic database search for the review was conducted in Pubmed/Medline. This literature search indicates that POI is quite common in systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Even though limited studies demonstrate that POI is also seen in systemic sclerosis, the underlying mechanism remains unclear. Therefore, further studies are needed to understand the influence of SSc on ovarian homeostasis.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125269245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.08
Mahnoor Javaid, Latika Gupta
Rapid aging of population in recent years has been accompanied by accumulation of multimorbid conditions. Chronic diseases often share common risk factors. As an example, autoimmune rheumatic diseases often evolve in combination with other autoimmune conditions. High rates of inflammatory disorders and rheumatic diseases are common multimorbidities in the elderly. A lack of practice guidelines for the management of multimorbidities, particularly in the elderly population and those with rare rheumatic diseases, has led to the inadequate treatment and added burden on the healthcare system. This opinion piece aims to draw attention on rising multimorbidity in the aging population prone to autoimmune rheumatic diseases and justify further research in this field.
{"title":"THE BERMUDA TRIANGLE: AGING, MULTIMORBIDITY, AND AUTOIMMUNE RHEUMATIC DISEASES","authors":"Mahnoor Javaid, Latika Gupta","doi":"10.56543/aaeeu.2022.1.2.08","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.08","url":null,"abstract":"Rapid aging of population in recent years has been accompanied by accumulation of multimorbid conditions. Chronic diseases often share common risk factors. As an example, autoimmune rheumatic diseases often evolve in combination with other autoimmune conditions. High rates of inflammatory disorders and rheumatic diseases are common multimorbidities in the elderly. A lack of practice guidelines for the management of multimorbidities, particularly in the elderly population and those with rare rheumatic diseases, has led to the inadequate treatment and added burden on the healthcare system. This opinion piece aims to draw attention on rising multimorbidity in the aging population prone to autoimmune rheumatic diseases and justify further research in this field.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133768678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.03
Z. Balik, Y. Bayındır, Ezgi Deniz Batu
Systemic lupus erythematosus (SLE) is an autoimmune disease affecting multiple systems and presenting with a wide range of clinical symptoms. Disease phenotype alters from childhood to old age. The possible reasons for these differences are genetic factors, changes in the hormonal status, and the aging immune system. As a result of the disease itself or as a side effect of medications, SLE patients often experience manifestations associated with aging like cardiovascular disease and infection susceptibility. Furthermore, immunosenescence, which commonly affects the elderly subjects, may result from the continued immune system activation during the natural history of SLE. In this study, we aimed to analyze the immunosenescence in SLE, the differences and similarities in SLE phenotype at different age groups, and possible reasons for these changes.
{"title":"MODELING OF IMMUNOSENESCENCE WITH AGING IN SYSTEMIC LUPUS ERYTHEMATOSUS","authors":"Z. Balik, Y. Bayındır, Ezgi Deniz Batu","doi":"10.56543/aaeeu.2022.1.2.03","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.03","url":null,"abstract":"Systemic lupus erythematosus (SLE) is an autoimmune disease affecting multiple systems and presenting with a wide range of clinical symptoms. Disease phenotype alters from childhood to old age. The possible reasons for these differences are genetic factors, changes in the hormonal status, and the aging immune system. As a result of the disease itself or as a side effect of medications, SLE patients often experience manifestations associated with aging like cardiovascular disease and infection susceptibility. Furthermore, immunosenescence, which commonly affects the elderly subjects, may result from the continued immune system activation during the natural history of SLE. In this study, we aimed to analyze the immunosenescence in SLE, the differences and similarities in SLE phenotype at different age groups, and possible reasons for these changes.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124139847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.07
U. Pidvalna, D. Beshley
Background. Aortic valve stenosis remains the most dominant form of valvular heart disease. The aortic valve area below 1.0 cm2 is an assignment to the interventions. The modern senile aortic valve stenosis treatment options are mini-surgical valve replacement (mini-SVR), balloon aortic valvuloplasty (BAV), and transcatheter aortic valve implantation (TAVI). This study aims to inform readers about up-to-date interventions for patients with senile calcific aortic valve stenosis in Ukraine, based on the experience of the Cardiac surgery department in Lviv, Ukraine. Methods. From a single-centre retrospective registry (10/2015-02/2022), 204 patients were included. One hundred seven patients underwent mini-SVR, tree BAV, and four – TAVI. Diagnostic modalities used to assess the anatomy of the aortic valve were: ECHO, ECG-gated computer tomography with aortic valve calcic scoring, and Angiography. Interventional procedure techniques were BAV, TAVI; surgical: mini-SVR via upper ministernotomy or right-sided minithoracotomy. Results. Anatomical assessment of the aortic valve in senile aortic valve stenosis was based on the morphology of the aortic valve (bicuspid or tricuspid aortic valve), asymmetrical hypertrophy of the left ventricular outflow tract, and coronary ostia height. The age-related anatomical features were calcium deposits in the leaflet, coronary ostium and mitral annular calcification. Ministernotomy (in 67 cases) and a right-sided minithoracotomy (in 40 cases) were performed to secure the “heart step” without instability of the chest cage with the smaller valve size implantation than was expected before. Preserving the chest cage and avoiding aortic cross-clamp/cardio-pulmonary bypass were advantages of TAVI. Fragile patients expected TAVI risks: aortic root damage, paravalvular leak, moderate aortic insufficient, the risk of atrioventricular block and embolic stroke, and kidney dysfunction. Conclusions. Mini-surgical valve replacement and transcatheter aortic valve implantation are accessible procedures in Ukraine for senile calcific aortic valve stenosis treatment.
{"title":"SENILE CALCIFIC AORTIC VALVE STENOSIS: MODERN INTERVENTIONS IN UKRAINE","authors":"U. Pidvalna, D. Beshley","doi":"10.56543/aaeeu.2022.1.2.07","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.07","url":null,"abstract":"Background. Aortic valve stenosis remains the most dominant form of valvular heart disease. The aortic valve area below 1.0 cm2 is an assignment to the interventions. The modern senile aortic valve stenosis treatment options are mini-surgical valve replacement (mini-SVR), balloon aortic valvuloplasty (BAV), and transcatheter aortic valve implantation (TAVI). This study aims to inform readers about up-to-date interventions for patients with senile calcific aortic valve stenosis in Ukraine, based on the experience of the Cardiac surgery department in Lviv, Ukraine. \u0000Methods. From a single-centre retrospective registry (10/2015-02/2022), 204 patients were included. One hundred seven patients underwent mini-SVR, tree BAV, and four – TAVI. Diagnostic modalities used to assess the anatomy of the aortic valve were: ECHO, ECG-gated computer tomography with aortic valve calcic scoring, and Angiography. Interventional procedure techniques were BAV, TAVI; surgical: mini-SVR via upper ministernotomy or right-sided minithoracotomy. \u0000Results. Anatomical assessment of the aortic valve in senile aortic valve stenosis was based on the morphology of the aortic valve (bicuspid or tricuspid aortic valve), asymmetrical hypertrophy of the left ventricular outflow tract, and coronary ostia height. The age-related anatomical features were calcium deposits in the leaflet, coronary ostium and mitral annular calcification. Ministernotomy (in 67 cases) and a right-sided minithoracotomy (in 40 cases) were performed to secure the “heart step” without instability of the chest cage with the smaller valve size implantation than was expected before. Preserving the chest cage and avoiding aortic cross-clamp/cardio-pulmonary bypass were advantages of TAVI. Fragile patients expected TAVI risks: aortic root damage, paravalvular leak, moderate aortic insufficient, the risk of atrioventricular block and embolic stroke, and kidney dysfunction. \u0000Conclusions. Mini-surgical valve replacement and transcatheter aortic valve implantation are accessible procedures in Ukraine for senile calcific aortic valve stenosis treatment.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134117885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.02
M. Prakashini, Sakir Ahmed
Infertility is emerging as one of the leading global health concerns. The spectrum of global diseases has shifted from communicable to non-communicable, with autoimmune diseases being at the crux of it. Infertility among females with autoimmune diseases has been explored. Many inflammatory arthritides affect men in their peak reproductive and functional age. However, data on male infertility in persons having these diseases is scarce. This topical review explores the multifactorial causation of male infertility in inflammatory arthritis. These individuals tend to have gonadal dysfunction, Sertoli cell dysfunction and hypotestosteronaemia. Individuals with rheumatoid arthritis and spondyloarthritis have also reported a loss of libido owing to poor quality of life, low functional status, and erectile dysfunction. These factors along with social and psychological factors greatly influence the development of persistent sexual ill-health. Another observation is that men who have primary infertility have a higher predisposition to developing autoimmune diseases; particularly in rheumatoid arthritis and spondyloarthritis. The morbidity that comes with pain and deformities resulting from inadequately treated disease or high disease activity, can also result in poor sexual well-being. Many of the males with inflammatory arthritis tend to be voluntarily childless due to the fear of passing the disease on to their off-springs and also a possible flare when the drug therapy is modified to facilitate conception. Male fertility in autoimmune rheumatic diseases is often a neglected topic in our practice and research. Physicians must be sensitized and receptive to the issues of infertility and sexual well-being in male patients with autoimmune rheumatic diseases.
{"title":"TESTOSTERONE AND ANDROPAUSE IN MALES WITH INFLAMMATORY ARTHRITIDES","authors":"M. Prakashini, Sakir Ahmed","doi":"10.56543/aaeeu.2022.1.2.02","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.02","url":null,"abstract":"Infertility is emerging as one of the leading global health concerns. The spectrum of global diseases has shifted from communicable to non-communicable, with autoimmune diseases being at the crux of it. Infertility among females with autoimmune diseases has been explored. Many inflammatory arthritides affect men in their peak reproductive and functional age. However, data on male infertility in persons having these diseases is scarce. \u0000This topical review explores the multifactorial causation of male infertility in inflammatory arthritis. These individuals tend to have gonadal dysfunction, Sertoli cell dysfunction and hypotestosteronaemia. Individuals with rheumatoid arthritis and spondyloarthritis have also reported a loss of libido owing to poor quality of life, low functional status, and erectile dysfunction. These factors along with social and psychological factors greatly influence the development of persistent sexual ill-health. Another observation is that men who have primary infertility have a higher predisposition to developing autoimmune diseases; particularly in rheumatoid arthritis and spondyloarthritis. \u0000The morbidity that comes with pain and deformities resulting from inadequately treated disease or high disease activity, can also result in poor sexual well-being. Many of the males with inflammatory arthritis tend to be voluntarily childless due to the fear of passing the disease on to their off-springs and also a possible flare when the drug therapy is modified to facilitate conception. \u0000Male fertility in autoimmune rheumatic diseases is often a neglected topic in our practice and research. Physicians must be sensitized and receptive to the issues of infertility and sexual well-being in male patients with autoimmune rheumatic diseases.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115417857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.10
S. Acer Kasman
COMBINING TRADITIONAL AND INNOVATIVE APPROACHES IN PHYSICIAL MEDICINE AND REHABILITATION
结合传统和创新的方法在物理医学和康复
{"title":"COMBINING TRADITIONAL AND INNOVATIVE APPROACHES IN PHYSICIAL MEDICINE AND REHABILITATION","authors":"S. Acer Kasman","doi":"10.56543/aaeeu.2022.1.2.10","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.10","url":null,"abstract":"COMBINING TRADITIONAL AND INNOVATIVE APPROACHES IN PHYSICIAL MEDICINE AND REHABILITATION","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"1960 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129385461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.01
Oleksii Bashkirtsev, V. Gaevska, O. Zimba, A. Gasparyan
Comprehensive and multidisciplinary rehabilitation is gaining momentum as a useful strategy that aims to improve physical, psychological, and social components of health in subjects affected by violence, trauma, and mental distress. Previous and current wars have prioritized essential diagnostic and rehabilitative services to civil subjects and military servicepersons which can be delivered by skilled physiatrists and allied specialists. Stratifying subjects in need of various rehabilitative procedures and offering them psychological support, balanced nutrition, musculoskeletal care, and socialisation in a safe and relaxing atmosphere may improve their mental and functional capacities and resolve numerous health issues. The choice of comprehensive rehabilitative procedures depends on their availability and understanding of complementary effects of various interventions.
{"title":"COMPREHENSIVE AND MULTIDISCIPLINARY REHABILITATION IN THE TIME OF CRISIS","authors":"Oleksii Bashkirtsev, V. Gaevska, O. Zimba, A. Gasparyan","doi":"10.56543/aaeeu.2022.1.2.01","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.01","url":null,"abstract":"Comprehensive and multidisciplinary rehabilitation is gaining momentum as a useful strategy that aims to improve physical, psychological, and social components of health in subjects affected by violence, trauma, and mental distress. Previous and current wars have prioritized essential diagnostic and rehabilitative services to civil subjects and military servicepersons which can be delivered by skilled physiatrists and allied specialists. Stratifying subjects in need of various rehabilitative procedures and offering them psychological support, balanced nutrition, musculoskeletal care, and socialisation in a safe and relaxing atmosphere may improve their mental and functional capacities and resolve numerous health issues. The choice of comprehensive rehabilitative procedures depends on their availability and understanding of complementary effects of various interventions.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134491452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.56543/aaeeu.2022.1.2.05
Tsvetoslav Georgiev, P. Kabakchieva
Osteoarthritis (OA) is traditionally considered an age-related disease. Therefore, repurposing drugs with the potential to reduce cell senescence is a justified therapeutic strategy. Such is the case of metformin, the most widely used antidiabetic medicine with well-known pharmacokinetics, acceptable toxicity, and beneficial metabolic effects. Metformin could significantly impact processes associated with aging and OA such as cellular senescence, infammaging, mitochondrial dysfunction and impaired nutrient sensing. The aim of the present narrative review is to unveil the potential of metformin to modify disease course in light of aging osteoarthritic joints. The drug has pleiotropic effects on chondrocyte and extracellular matrix metabolism and may provide through AMPK-dependent and -independent pathways a meaningful improvement of OA. Mostly preclinical and retrospective cohort studies have shown that metformin exposure could lead to the regulation of cartilage homeostasis, symptomatic relief of pain and postpone surgery for those suffering from OA. Randomized control trials are warranted to justify the preliminary expectations.
{"title":"METFORMIN AS A PROMISING ANTI-AGING AGENT IN THE TREATMENT OF OSTEOARTHRITIS","authors":"Tsvetoslav Georgiev, P. Kabakchieva","doi":"10.56543/aaeeu.2022.1.2.05","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.2.05","url":null,"abstract":"Osteoarthritis (OA) is traditionally considered an age-related disease. Therefore, repurposing drugs with the potential to reduce cell senescence is a justified therapeutic strategy. Such is the case of metformin, the most widely used antidiabetic medicine with well-known pharmacokinetics, acceptable toxicity, and beneficial metabolic effects. Metformin could significantly impact processes associated with aging and OA such as cellular senescence, infammaging, mitochondrial dysfunction and impaired nutrient sensing. The aim of the present narrative review is to unveil the potential of metformin to modify disease course in light of aging osteoarthritic joints. The drug has pleiotropic effects on chondrocyte and extracellular matrix metabolism and may provide through AMPK-dependent and -independent pathways a meaningful improvement of OA. Mostly preclinical and retrospective cohort studies have shown that metformin exposure could lead to the regulation of cartilage homeostasis, symptomatic relief of pain and postpone surgery for those suffering from OA. Randomized control trials are warranted to justify the preliminary expectations.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121171971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.11
Vitalii Kyryk, O. Kuchuk, P. Klymenko
Background. Adipose-derived stem cells (ADSCs) are a promising source for the regeneration of bone tissue injuries. At the same time, three-dimensional cultures provide spatial organization of stem cells for optimal intercellular signaling, contact interaction and increase the efficiency of directed osteogenic differentiation prior to further transplantation. The aim of the study was to establish the regenerative potential of mouse adipose-derived stem cells in micromass grafts differentiated into the osteogenic direction to restore the bone injury in mice. Methods. Three-dimensional micromass cultures of murine ADSCs with further differentiation into osteogenic direction were obtained. The migration potential of cells from micromass in vitro and the effectiveness of differentiation by staining for alkaline phosphatase were evaluated. Mice with the model of femoral bone injury were transplanted with ADSCs micromass grafts and 21 days later the lesion site was examined by histological and morphometric methods. Results. The protocols for the cultivation and directed osteogenic differentiation of ADSCs in the three-dimensional micromass culture have been developed. Alkaline phosphatase production was demonstrated in cells that migrated from micromass, confirming the effectiveness of differentiation. In macroscopic examination 21 days after graft transplantation, the defect sites in femur were filled with dense tissue, while in control bones without the use of transplants, the size of the defect by 80 ± 6 % corresponded to the initial diameter and depth of injury. Histological examination of femoral bone lesions in the area of transplantation of micromass grafts revealed the formation of granulation tissue followed by the replacement of defects with newly formed bone tissue with thickening of periosteum and compact bone substance, similar to callus in fracture regeneration. In animals that underwent transplantation of micromass without prior osteogenic differentiation, the diameter of the zone of active regeneration of the diaphysis at the site of injury was 1.3 ± 0.2 mm while in the group with transplantation of directed differentiated graft it was significantly lower (0.37 ± 0.12 mm, p ≤ 0.05). Conclusions. Three-dimensional grafts of adipose-derived multipotent mesenchymal stromal cells cultured in micromass are able to improve bone tissue regeneration in a model of bone injury in mice. In this case, the grafts differentiated into osteogenic direction, provide better morphological indicators of bone recovery, compared with the micromass without prior differentiation.
背景。脂肪源性干细胞(ADSCs)是骨组织损伤再生的一个有前景的来源。同时，三维培养为干细胞提供了最佳的细胞间信号传导和接触相互作用的空间组织，并在进一步移植之前提高了定向成骨分化的效率。本研究的目的是建立小鼠脂肪源性干细胞在成骨方向分化的微块移植物中的再生潜能，以恢复小鼠骨损伤。方法。获得小鼠ADSCs的三维微块培养，并进一步向成骨方向分化。用碱性磷酸酶染色法评价微团细胞在体外的迁移潜力和分化效果。用ADSCs微块移植股骨骨损伤模型小鼠，21 d后用组织学和形态计量学方法观察损伤部位。结果。建立了三维微块培养中ADSCs的定向成骨分化方法。碱性磷酸酶在从微团迁移的细胞中产生，证实了分化的有效性。在移植21天后的宏观检查中，股骨缺损部位充满了致密的组织，而在未使用移植的对照骨中，缺损的大小为80±6%，与损伤的初始直径和深度相对应。微块移植物移植区股骨病变组织学检查显示，肉芽组织形成，缺损被新形成的骨组织取代，骨膜增厚，骨物质致密，类似骨折再生中的骨痂。未进行成骨分化的小块移植组损伤部位骨干主动再生区直径为1.3±0.2 mm，而定向分化移植组损伤部位骨干主动再生区直径明显低于对照组(0.37±0.12 mm, p≤0.05)。结论。脂肪来源的多能间充质间质细胞微团培养的三维移植物能够改善小鼠骨损伤模型中的骨组织再生。在这种情况下，移植物向成骨方向分化，与未分化的微块相比，提供了更好的骨恢复形态学指标。
{"title":"REGENERATIVE EFFECTS OF MOUSE ADIPOSE-DERIVED MULTIPOTENT STROMAL CELLS IN A MICROMASS GRAFT FOR THE TREATMENT OF BONE INJURY MODEL","authors":"Vitalii Kyryk, O. Kuchuk, P. Klymenko","doi":"10.56543/aaeeu.2022.1.1.11","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.11","url":null,"abstract":"Background. Adipose-derived stem cells (ADSCs) are a promising source for the regeneration of bone tissue injuries. At the same time, three-dimensional cultures provide spatial organization of stem cells for optimal intercellular signaling, contact interaction and increase the efficiency of directed osteogenic differentiation prior to further transplantation. \u0000The aim of the study was to establish the regenerative potential of mouse adipose-derived stem cells in micromass grafts differentiated into the osteogenic direction to restore the bone injury in mice. \u0000Methods. Three-dimensional micromass cultures of murine ADSCs with further differentiation into osteogenic direction were obtained. The migration potential of cells from micromass in vitro and the effectiveness of differentiation by staining for alkaline phosphatase were evaluated. Mice with the model of femoral bone injury were transplanted with ADSCs micromass grafts and 21 days later the lesion site was examined by histological and morphometric methods. \u0000Results. The protocols for the cultivation and directed osteogenic differentiation of ADSCs in the three-dimensional micromass culture have been developed. Alkaline phosphatase production was demonstrated in cells that migrated from micromass, confirming the effectiveness of differentiation. In macroscopic examination 21 days after graft transplantation, the defect sites in femur were filled with dense tissue, while in control bones without the use of transplants, the size of the defect by 80 ± 6 % corresponded to the initial diameter and depth of injury. Histological examination of femoral bone lesions in the area of transplantation of micromass grafts revealed the formation of granulation tissue followed by the replacement of defects with newly formed bone tissue with thickening of periosteum and compact bone substance, similar to callus in fracture regeneration. In animals that underwent transplantation of micromass without prior osteogenic differentiation, the diameter of the zone of active regeneration of the diaphysis at the site of injury was 1.3 ± 0.2 mm while in the group with transplantation of directed differentiated graft it was significantly lower (0.37 ± 0.12 mm, p ≤ 0.05). \u0000Conclusions. Three-dimensional grafts of adipose-derived multipotent mesenchymal stromal cells cultured in micromass are able to improve bone tissue regeneration in a model of bone injury in mice. In this case, the grafts differentiated into osteogenic direction, provide better morphological indicators of bone recovery, compared with the micromass without prior differentiation.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124616415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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