Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.11
Vitalii Kyryk, O. Kuchuk, P. Klymenko
Background. Adipose-derived stem cells (ADSCs) are a promising source for the regeneration of bone tissue injuries. At the same time, three-dimensional cultures provide spatial organization of stem cells for optimal intercellular signaling, contact interaction and increase the efficiency of directed osteogenic differentiation prior to further transplantation. The aim of the study was to establish the regenerative potential of mouse adipose-derived stem cells in micromass grafts differentiated into the osteogenic direction to restore the bone injury in mice. Methods. Three-dimensional micromass cultures of murine ADSCs with further differentiation into osteogenic direction were obtained. The migration potential of cells from micromass in vitro and the effectiveness of differentiation by staining for alkaline phosphatase were evaluated. Mice with the model of femoral bone injury were transplanted with ADSCs micromass grafts and 21 days later the lesion site was examined by histological and morphometric methods. Results. The protocols for the cultivation and directed osteogenic differentiation of ADSCs in the three-dimensional micromass culture have been developed. Alkaline phosphatase production was demonstrated in cells that migrated from micromass, confirming the effectiveness of differentiation. In macroscopic examination 21 days after graft transplantation, the defect sites in femur were filled with dense tissue, while in control bones without the use of transplants, the size of the defect by 80 ± 6 % corresponded to the initial diameter and depth of injury. Histological examination of femoral bone lesions in the area of transplantation of micromass grafts revealed the formation of granulation tissue followed by the replacement of defects with newly formed bone tissue with thickening of periosteum and compact bone substance, similar to callus in fracture regeneration. In animals that underwent transplantation of micromass without prior osteogenic differentiation, the diameter of the zone of active regeneration of the diaphysis at the site of injury was 1.3 ± 0.2 mm while in the group with transplantation of directed differentiated graft it was significantly lower (0.37 ± 0.12 mm, p ≤ 0.05). Conclusions. Three-dimensional grafts of adipose-derived multipotent mesenchymal stromal cells cultured in micromass are able to improve bone tissue regeneration in a model of bone injury in mice. In this case, the grafts differentiated into osteogenic direction, provide better morphological indicators of bone recovery, compared with the micromass without prior differentiation.
背景。脂肪源性干细胞(ADSCs)是骨组织损伤再生的一个有前景的来源。同时，三维培养为干细胞提供了最佳的细胞间信号传导和接触相互作用的空间组织，并在进一步移植之前提高了定向成骨分化的效率。本研究的目的是建立小鼠脂肪源性干细胞在成骨方向分化的微块移植物中的再生潜能，以恢复小鼠骨损伤。方法。获得小鼠ADSCs的三维微块培养，并进一步向成骨方向分化。用碱性磷酸酶染色法评价微团细胞在体外的迁移潜力和分化效果。用ADSCs微块移植股骨骨损伤模型小鼠，21 d后用组织学和形态计量学方法观察损伤部位。结果。建立了三维微块培养中ADSCs的定向成骨分化方法。碱性磷酸酶在从微团迁移的细胞中产生，证实了分化的有效性。在移植21天后的宏观检查中，股骨缺损部位充满了致密的组织，而在未使用移植的对照骨中，缺损的大小为80±6%，与损伤的初始直径和深度相对应。微块移植物移植区股骨病变组织学检查显示，肉芽组织形成，缺损被新形成的骨组织取代，骨膜增厚，骨物质致密，类似骨折再生中的骨痂。未进行成骨分化的小块移植组损伤部位骨干主动再生区直径为1.3±0.2 mm，而定向分化移植组损伤部位骨干主动再生区直径明显低于对照组(0.37±0.12 mm, p≤0.05)。结论。脂肪来源的多能间充质间质细胞微团培养的三维移植物能够改善小鼠骨损伤模型中的骨组织再生。在这种情况下，移植物向成骨方向分化，与未分化的微块相比，提供了更好的骨恢复形态学指标。
{"title":"REGENERATIVE EFFECTS OF MOUSE ADIPOSE-DERIVED MULTIPOTENT STROMAL CELLS IN A MICROMASS GRAFT FOR THE TREATMENT OF BONE INJURY MODEL","authors":"Vitalii Kyryk, O. Kuchuk, P. Klymenko","doi":"10.56543/aaeeu.2022.1.1.11","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.11","url":null,"abstract":"Background. Adipose-derived stem cells (ADSCs) are a promising source for the regeneration of bone tissue injuries. At the same time, three-dimensional cultures provide spatial organization of stem cells for optimal intercellular signaling, contact interaction and increase the efficiency of directed osteogenic differentiation prior to further transplantation. \u0000The aim of the study was to establish the regenerative potential of mouse adipose-derived stem cells in micromass grafts differentiated into the osteogenic direction to restore the bone injury in mice. \u0000Methods. Three-dimensional micromass cultures of murine ADSCs with further differentiation into osteogenic direction were obtained. The migration potential of cells from micromass in vitro and the effectiveness of differentiation by staining for alkaline phosphatase were evaluated. Mice with the model of femoral bone injury were transplanted with ADSCs micromass grafts and 21 days later the lesion site was examined by histological and morphometric methods. \u0000Results. The protocols for the cultivation and directed osteogenic differentiation of ADSCs in the three-dimensional micromass culture have been developed. Alkaline phosphatase production was demonstrated in cells that migrated from micromass, confirming the effectiveness of differentiation. In macroscopic examination 21 days after graft transplantation, the defect sites in femur were filled with dense tissue, while in control bones without the use of transplants, the size of the defect by 80 ± 6 % corresponded to the initial diameter and depth of injury. Histological examination of femoral bone lesions in the area of transplantation of micromass grafts revealed the formation of granulation tissue followed by the replacement of defects with newly formed bone tissue with thickening of periosteum and compact bone substance, similar to callus in fracture regeneration. In animals that underwent transplantation of micromass without prior osteogenic differentiation, the diameter of the zone of active regeneration of the diaphysis at the site of injury was 1.3 ± 0.2 mm while in the group with transplantation of directed differentiated graft it was significantly lower (0.37 ± 0.12 mm, p ≤ 0.05). \u0000Conclusions. Three-dimensional grafts of adipose-derived multipotent mesenchymal stromal cells cultured in micromass are able to improve bone tissue regeneration in a model of bone injury in mice. In this case, the grafts differentiated into osteogenic direction, provide better morphological indicators of bone recovery, compared with the micromass without prior differentiation.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124616415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.10
K. Jagtap, L. Hoff, E. Conticini, R. Naveen, Latika Gupta
Ageing is associated with a progressive decline in muscle mass and quality. Inflammaging, chronic low-grade inflammation is a major causative as well as maintenance factor in age-related disorders. Idiopathic inflammatory myopathies or myositis also exhibit a chronic stage of inflammation caused by various immune and non-immune-mediated processes. This review draws parallels between the mechanisms of inflammaging, sarcopenia, and myositis, and their possible interconnection. We searched literature on information about myositis, sarcopenia, ageing, inflammaging, and senescence to draw parallels between the mechanisms linking myositis, sarcopenia, and inflammaging. Further, we discuss the evidence base to support that the process of senescence is hastened in an inflamed muscle [1].
{"title":"INFLAMMAGING IN MUSCLE: THE MISSING LINK BETWEEN SARCOPENIA AND IDIOPATHIC INFLAMMATORY MYOPATHIES","authors":"K. Jagtap, L. Hoff, E. Conticini, R. Naveen, Latika Gupta","doi":"10.56543/aaeeu.2022.1.1.10","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.10","url":null,"abstract":"Ageing is associated with a progressive decline in muscle mass and quality. Inflammaging, chronic low-grade inflammation is a major causative as well as maintenance factor in age-related disorders. Idiopathic inflammatory myopathies or myositis also exhibit a chronic stage of inflammation caused by various immune and non-immune-mediated processes. This review draws parallels between the mechanisms of inflammaging, sarcopenia, and myositis, and their possible interconnection. \u0000We searched literature on information about myositis, sarcopenia, ageing, inflammaging, and senescence to draw parallels between the mechanisms linking myositis, sarcopenia, and inflammaging. Further, we discuss the evidence base to support that the process of senescence is hastened in an inflamed muscle [1].","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"207 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114364728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.01
Oleksii Bashkirtsev, O. Zimba
AGING AND ANTI-AGING RESEARCH AND PUBLISHING PRIORITIES
衰老和抗衰老的研究和出版重点
{"title":"AGING AND ANTI-AGING RESEARCH AND PUBLISHING PRIORITIES","authors":"Oleksii Bashkirtsev, O. Zimba","doi":"10.56543/aaeeu.2022.1.1.01","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.01","url":null,"abstract":"AGING AND ANTI-AGING RESEARCH AND PUBLISHING PRIORITIES","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134332729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.12
I. Benlidayı
POLYPHARMACY AS A THREAT TO AGING POPULATION
多重药房是人口老龄化的威胁
{"title":"POLYPHARMACY AS A THREAT TO AGING POPULATION","authors":"I. Benlidayı","doi":"10.56543/aaeeu.2022.1.1.12","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.12","url":null,"abstract":"POLYPHARMACY AS A THREAT TO AGING POPULATION","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116772306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.02
B. Koçyiğit
COVID-19 is an infectious disease that can have a multi-system involvement, most notably, the respiratory tract. After acute infection, a considerable proportion of patients suffer from persistent symptoms and signs, defined as long COVID-19. Depending on the affected systems and organs, patients can experience various clinic pictures. Rehabilitation approaches serve a crucial role in providing functional recovery and improving quality of life for COVID-19 survivors. As a result of the wideness of the clinical spectrum of the long COVID-19, rehabilitation practices differ according to the affected systems. Therefore, the formation of multidisciplinary rehabilitation teams is an inevitable necessity. The establishment of specific centers for long COVID-19 rehabilitation will be beneficial. If it is not possible, existing rehabilitation centers should be strengthened for this purpose. Since the pulmonary system and respiratory tract are the most affected structures, one of the main issues to be considered in long COVID-19 cases is cardiopulmonary rehabilitation. Patients experience musculoskeletal disorders such as atrophy, sarcopenia, poor physical performance and contracture due to long-term hospitalization, intensive care treatment, quarantine practices and immobilization. Rehabilitation practices also focus on these disorders. All rehabilitation practices in long COVID-19 patients should start with low intensity, and parameters such as intensity and frequency should be increased as the patient's tolerance improves. In high-risk cases, parameters including oxygen saturation, blood pressure, and heart rhythm should be monitored. Health-care authorities should prioritize the rehabilitation of the long COVID-19 syndrome and invest in this area. Authorities, physicians and patients should collaborate to facilitate long COVID-19 rehabilitation and to establish a self-contained system.
{"title":"THE ROLE OF PHYSICAL MEDICINE AND REHABILITATION IN LONG COVID-19 MANAGEMENT","authors":"B. Koçyiğit","doi":"10.56543/aaeeu.2022.1.1.02","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.02","url":null,"abstract":"COVID-19 is an infectious disease that can have a multi-system involvement, most notably, the respiratory tract. After acute infection, a considerable proportion of patients suffer from persistent symptoms and signs, defined as long COVID-19. Depending on the affected systems and organs, patients can experience various clinic pictures. Rehabilitation approaches serve a crucial role in providing functional recovery and improving quality of life for COVID-19 survivors. As a result of the wideness of the clinical spectrum of the long COVID-19, rehabilitation practices differ according to the affected systems. Therefore, the formation of multidisciplinary rehabilitation teams is an inevitable necessity. The establishment of specific centers for long COVID-19 rehabilitation will be beneficial. If it is not possible, existing rehabilitation centers should be strengthened for this purpose. Since the pulmonary system and respiratory tract are the most affected structures, one of the main issues to be considered in long COVID-19 cases is cardiopulmonary rehabilitation. Patients experience musculoskeletal disorders such as atrophy, sarcopenia, poor physical performance and contracture due to long-term hospitalization, intensive care treatment, quarantine practices and immobilization. Rehabilitation practices also focus on these disorders. All rehabilitation practices in long COVID-19 patients should start with low intensity, and parameters such as intensity and frequency should be increased as the patient's tolerance improves. In high-risk cases, parameters including oxygen saturation, blood pressure, and heart rhythm should be monitored. Health-care authorities should prioritize the rehabilitation of the long COVID-19 syndrome and invest in this area. Authorities, physicians and patients should collaborate to facilitate long COVID-19 rehabilitation and to establish a self-contained system.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125815958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.07
N. Gokcen
Aging is associated with deterioration of the immune function. Two contributory mechanisms are inflamm-aging, which is a chronic, low-grade systemic inflammation, and immunosenescence, an impairment of adaptive immune function that may also contribute to the development of inflamm-aging. This age-related inflammatory event is associated with alteration to the balance of pro-inflammatory and anti-inflammatory cytokines. The effect of inflamm-aging on skin aging in healthy people is accepted; however, its effect on normal skin aging and/or skin characteristics in systemic sclerosis is unknown. The hypothesis presented herein suggests that inflamm-aging may contribute to the evolution of the skin phases in systemic sclerosis, which progress from edematous, fibrotic, and indurative phases to the atrophic phase.
{"title":"INFLAMM-AGING: A MECHANISM OF AGING THAT CONTRIBUTES TO THE CHARACTERISTICS OF SKIN INVOLVEMENT IN SYSTEMIC SCLEROSIS","authors":"N. Gokcen","doi":"10.56543/aaeeu.2022.1.1.07","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.07","url":null,"abstract":"Aging is associated with deterioration of the immune function. Two contributory mechanisms are inflamm-aging, which is a chronic, low-grade systemic inflammation, and immunosenescence, an impairment of adaptive immune function that may also contribute to the development of inflamm-aging. This age-related inflammatory event is associated with alteration to the balance of pro-inflammatory and anti-inflammatory cytokines. The effect of inflamm-aging on skin aging in healthy people is accepted; however, its effect on normal skin aging and/or skin characteristics in systemic sclerosis is unknown. The hypothesis presented herein suggests that inflamm-aging may contribute to the evolution of the skin phases in systemic sclerosis, which progress from edematous, fibrotic, and indurative phases to the atrophic phase.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126970485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.08
M. Goyal, Arun Kumar Kedia
Vitamin D has anti-inflammatory and pro-autophagy actions and influences the genetic and epigenetic landscape to promote healthy aging. A deficiency of this vitamin leads to accelerated aging. Deficiency of vitamin D causes sarcopenia, osteoporosis, frailty and a high risk of fractures and consequently high morbidity. To complicate matters, in the elderly, various factors like reduced dietary intake, reduced mobility and sun exposure, decreased production and activation of vitamin D, reduction in the population of vitamin D receptors and diminished responsiveness of tissues to vitamin D cause reduced vitamin D levels and function. The above factors indicate that a special considered approach be adopted for the prevention and treatment of vitamin D deficiency in the elderly.
{"title":"VITAMIN D AND AGING: AN INTERPLAY OF MULTIPLE MECHANISMS","authors":"M. Goyal, Arun Kumar Kedia","doi":"10.56543/aaeeu.2022.1.1.08","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.08","url":null,"abstract":"Vitamin D has anti-inflammatory and pro-autophagy actions and influences the genetic and epigenetic landscape to promote healthy aging. A deficiency of this vitamin leads to accelerated aging. Deficiency of vitamin D causes sarcopenia, osteoporosis, frailty and a high risk of fractures and consequently high morbidity. To complicate matters, in the elderly, various factors like reduced dietary intake, reduced mobility and sun exposure, decreased production and activation of vitamin D, reduction in the population of vitamin D receptors and diminished responsiveness of tissues to vitamin D cause reduced vitamin D levels and function. The above factors indicate that a special considered approach be adopted for the prevention and treatment of vitamin D deficiency in the elderly.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126957247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.05
D. Cansu, C. Korkmaz
Familial Mediterranean fever (FMF) is an autoinflammatory disease that causes recurrent fever and serositis. FMF often begins in childhood and is diagnosed at an early age. Although it is uncommon for the disease to occur after the age of 40, late-onset patient series have been published and compared to early-onset patient series in recent years. Although it is a genetically inherited disease, the reason why clinical symptoms appear at such a late age in some patients is unknown. The frequency of pathogenic mutations is lower in these patients than in early-onset FMF patients, and the disease has a milder course. Whether or not this clinical presentation is related to immune system changes associated with aging is an open question. Age-related immune system changes, such as an increase in senescence cells, the development of senescence-associated secretory phenotype, and a decline in autophagy with age, can trigger the inflammasome activation. In this regard, understanding the cause of the late-onset of FMF attacks may open up new avenues for research into pathogenesis. In this review, we will first compare the clinical features of the early and late-onset FMF series. We will then consider hypothetical causes of late-onset FMF attacks by reviewing age-related changes in the innate immune system.
{"title":"IMMUNOSENESCENCE AND LATE-ONSET FAMILIAL MEDITERRANEAN FEVER","authors":"D. Cansu, C. Korkmaz","doi":"10.56543/aaeeu.2022.1.1.05","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.05","url":null,"abstract":"Familial Mediterranean fever (FMF) is an autoinflammatory disease that causes recurrent fever and serositis. FMF often begins in childhood and is diagnosed at an early age. Although it is uncommon for the disease to occur after the age of 40, late-onset patient series have been published and compared to early-onset patient series in recent years. Although it is a genetically inherited disease, the reason why clinical symptoms appear at such a late age in some patients is unknown. The frequency of pathogenic mutations is lower in these patients than in early-onset FMF patients, and the disease has a milder course. Whether or not this clinical presentation is related to immune system changes associated with aging is an open question. Age-related immune system changes, such as an increase in senescence cells, the development of senescence-associated secretory phenotype, and a decline in autophagy with age, can trigger the inflammasome activation. In this regard, understanding the cause of the late-onset of FMF attacks may open up new avenues for research into pathogenesis. In this review, we will first compare the clinical features of the early and late-onset FMF series. We will then consider hypothetical causes of late-onset FMF attacks by reviewing age-related changes in the innate immune system.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"28 9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116292634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.56543/aaeeu.2022.1.1.03
O. Kalmukova, Vitalii Kyryk, M. Dzerzhynsky
Background. Nowadays personalized medicine is actively developing and consists of individual approaches during patients' treatment, diagnoses and prognoses. Since the first use of DNA sequence analysis in 2009, many other directions and methods for precision medicine have been proposed, including metabolome, transcriptome, proteome, microbiome analysis etc., which reflect internal factors of organisms. Moreover, to take into account environmental influence on organisms including day/night activity, feeding and physical training regime, it was proposed to apply the descriptions of circadian system rhythmicity of each patient. Also, with organism aging, the sensitivity to external factors is raised that emphasizes the importance of the chronobiological approach in anti-aging concept. In this review we discussed available ways of the application of circadian system parameters to analyze human metabolic state. Methods. Search strategy: PubMed, Scopus, DOAJ (Directory of Open Access Journals) and Google Scholar were used to search for original research and articles review; no abstracts from meeting reports have been cited. ClinicalTrials.gov was used to search for clinical studies. Search terms included “chronotherapy”, “circadian system”, and “chronobiology”. Results. According to personalized medicine, the analysis of circadian system in the case of each patient is necessary as circadian rhythmicity varies in every person. Taking into account the peculiarities of patient’s circadian system it will be easy to choose the best time for drug administration resulting in high efficacy and low side effects. The analysis of circadian system can be performed on molecular, physiological and systemic (general, metabolic and inflammation markers) levels. There was shown the increase in the number of clinical trials which are based on the use of chronobiological approach during the treatment of different pathologies that increase with aging: depression, insomnia, metabolic and cardiovascular disease, cancer. More than 1,000 clinical trials involving circadian interventions and chronobiology have been registered worldwide. Conclusion. Chronobiological approach can be used as an additional measure to anti-aging therapy to diagnose metabolic state, to choose more effective treatment time as well as in preventive healthcare in terms of personalized medicine.
{"title":"CIRCADIAN RHYTHMS AND PERSONALIZED STRATEGIES FOR ANTI-AGING THERAPIES","authors":"O. Kalmukova, Vitalii Kyryk, M. Dzerzhynsky","doi":"10.56543/aaeeu.2022.1.1.03","DOIUrl":"https://doi.org/10.56543/aaeeu.2022.1.1.03","url":null,"abstract":"Background. Nowadays personalized medicine is actively developing and consists of individual approaches during patients' treatment, diagnoses and prognoses. Since the first use of DNA sequence analysis in 2009, many other directions and methods for precision medicine have been proposed, including metabolome, transcriptome, proteome, microbiome analysis etc., which reflect internal factors of organisms. Moreover, to take into account environmental influence on organisms including day/night activity, feeding and physical training regime, it was proposed to apply the descriptions of circadian system rhythmicity of each patient. Also, with organism aging, the sensitivity to external factors is raised that emphasizes the importance of the chronobiological approach in anti-aging concept. In this review we discussed available ways of the application of circadian system parameters to analyze human metabolic state. \u0000Methods. Search strategy: PubMed, Scopus, DOAJ (Directory of Open Access Journals) and Google Scholar were used to search for original research and articles review; no abstracts from meeting reports have been cited. ClinicalTrials.gov was used to search for clinical studies. Search terms included “chronotherapy”, “circadian system”, and “chronobiology”. \u0000Results. According to personalized medicine, the analysis of circadian system in the case of each patient is necessary as circadian rhythmicity varies in every person. Taking into account the peculiarities of patient’s circadian system it will be easy to choose the best time for drug administration resulting in high efficacy and low side effects. The analysis of circadian system can be performed on molecular, physiological and systemic (general, metabolic and inflammation markers) levels. There was shown the increase in the number of clinical trials which are based on the use of chronobiological approach during the treatment of different pathologies that increase with aging: depression, insomnia, metabolic and cardiovascular disease, cancer. More than 1,000 clinical trials involving circadian interventions and chronobiology have been registered worldwide. \u0000Conclusion. Chronobiological approach can be used as an additional measure to anti-aging therapy to diagnose metabolic state, to choose more effective treatment time as well as in preventive healthcare in terms of personalized medicine.","PeriodicalId":437966,"journal":{"name":"Anti-Aging Eastern Europe","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124302346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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