L. Wittlinger, L. Petrikovičová, F. Petrovič, J. Petrikovič
Biological systems are subject to a dramatic increase in invading species in the 21st century due to increasing globalization around the world. In the near future, these species will cause more extensive ecological as well as socio-economic damage. Biotic invasions will result not only in a reduction of the original biodiversity, but also total loss, particularly degradation of natural habitats – especially Natura 2000 habitats. This study aims to understand the processes of spread of invasive plant species, their way of life, adaptation to environmental changes and displacement of native species. Without the local level, it is not possible to understand these processes on the regional and continental dimensions and thus implement scientific facts and findings into the prediction of the development of global biosystems. The research was conducted during the vegetation period in the years 2017–2021 in the studied areas of non-forest and forest habitats in the central part of Slovakia. We recorded 242 localities in the area with the occurrence of invasive plant species in an area of 3.057 km2 out of the total mapped area of 169.024 km2. We recorded the highest number of localities and the most expansive distribution for the species Stenactis annua, Robinia pseudoacacia, Solidago canadensis, Impatiens glandulifera and Fallopia japonica.
{"title":"Geographical distribution and spatio-temporal changes in the occurrence of invasive plant species in Slovak Republic","authors":"L. Wittlinger, L. Petrikovičová, F. Petrovič, J. Petrikovič","doi":"10.15421/012211","DOIUrl":"https://doi.org/10.15421/012211","url":null,"abstract":"Biological systems are subject to a dramatic increase in invading species in the 21st century due to increasing globalization around the world. In the near future, these species will cause more extensive ecological as well as socio-economic damage. Biotic invasions will result not only in a reduction of the original biodiversity, but also total loss, particularly degradation of natural habitats – especially Natura 2000 habitats. This study aims to understand the processes of spread of invasive plant species, their way of life, adaptation to environmental changes and displacement of native species. Without the local level, it is not possible to understand these processes on the regional and continental dimensions and thus implement scientific facts and findings into the prediction of the development of global biosystems. The research was conducted during the vegetation period in the years 2017–2021 in the studied areas of non-forest and forest habitats in the central part of Slovakia. We recorded 242 localities in the area with the occurrence of invasive plant species in an area of 3.057 km2 out of the total mapped area of 169.024 km2. We recorded the highest number of localities and the most expansive distribution for the species Stenactis annua, Robinia pseudoacacia, Solidago canadensis, Impatiens glandulifera and Fallopia japonica.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"25 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73261692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Findings of naked amoebae in various habitats of Ukraine and other territories are presented. Saccamoeba sp., Thecamoeba similis (Greeff, 1891) Lepşi, 1960, Vexillifera bacillipedes Page, 1969, Vannella lata Page, 1988, Acanthamoeba sp., Willaertia magna De Jonckheere, Dive, Pussard & Vickerman, 1984 were identified based on morphological data and molecular genetic research methods. All these species of amoebae are characterized according to our own observations and measurements and the published data. The amoebae we found belong to three classes (Tubulinea, Discosea, Heterolobosea), six orders (Euamoebida, Thecamoebida, Dactylopodida, Vannellida, Centramoebida, Schizopyrenida), six families (Hartmannellidae, Thecamoebidae, Vexilliferidae, Vannellidae, Acanthamoebidae, Vahlkampfiidae) and six genera (Saccamoeba, Thecamoeba, Vexillifera, Vannella, Acanthamoeba, Willaertia). W. magna was the least common in water bodies of different regions of Ukraine; Saccamoeba sp., V. bacillipedes in the studied water bodies are either uncommon species or occupy the middle position in terms of frequency of occurrence In the water bodies of Ukraine T. similis, V. lata, Acanthamoeba sp. are both the least common and the most common species, or occupy the middle position in terms of frequency of occurrence. The following ecological groups of naked amoebae have been identified in relation to temperature: eurythermal, psychrophilic stenothermal, thermophilic stenothermal. In relation to the concentration of dissolved oxygen in water: euryoxidic and stenooxidic. Three species of amoebae can withstand either a wide range of values of organic substances dissolved in water, or belong to stenobiont species. The recorded amoebae belong to monotactic (Saccamoeba sp.), striate (T. similis), dactylopodial (V. bacillipedes), fan-shaped (V. lata), acanthopodial (Acanthamoeba sp.) and eruptive (W. magna) morphotypes.
本文介绍了在乌克兰和其他地区不同生境中发现的裸变形虫。根据形态学资料和分子遗传学研究方法鉴定出sacamoeba sp.， thecacacamoeba similis (Greeff, 1891) lep， 1960, Vexillifera bacillipedes Page, 1969, Vannella lata Page, 1988，棘阿米巴sp.， Willaertia magna De Jonckheere, Dive, Pussard & Vickerman, 1984。所有这些变形虫物种都是根据我们自己的观察和测量以及发表的数据来特征的。我们发现的变形虫隶属于3个纲(管状亚纲、盘状亚纲、杂色亚纲)，6个目(真变形虫亚目、拟变形虫亚目、Dactylopodida、万尼亚目、拟拟变形虫亚目、拟拟变形虫亚目、拟拟变形虫亚目、拟拟变形虫亚目、拟拟变形虫亚目)，6个属(sacamoeba、拟拟变形虫亚目、拟拟变形虫亚目、拟拟变形虫亚目、拟拟变形虫亚目、拟拟变形虫亚目、拟拟变形虫亚目)。W. magna在乌克兰不同地区的水体中最不常见;在研究水体中，sacamoeba sp.、V. bacillipedes要么是不常见的物种，要么在出现频率上占据中间位置。乌克兰T. similis水体中，V. lata、Acanthamoeba sp.是最不常见的物种，也是最常见的物种，或者在出现频率上占据中间位置。根据温度的关系，裸变形虫的生态类群已被确定为:常温、嗜湿恒温、嗜热恒温。关于溶解氧在水中的浓度:泛氧和稀氧。三种变形虫要么能承受很大范围内溶解在水中的有机物质，要么属于窄生物种。记录的阿米巴属单形(sacamoeba sp.)、条纹形(T. similis)、趾型(V. bacillipedes)、扇形(V. lata)、棘足型(Acanthamoeba sp.)和爆发型(W. magna)。
{"title":"New finds of naked amoebae","authors":"M. Patsyuk","doi":"10.15421/012216","DOIUrl":"https://doi.org/10.15421/012216","url":null,"abstract":"Findings of naked amoebae in various habitats of Ukraine and other territories are presented. Saccamoeba sp., Thecamoeba similis (Greeff, 1891) Lepşi, 1960, Vexillifera bacillipedes Page, 1969, Vannella lata Page, 1988, Acanthamoeba sp., Willaertia magna De Jonckheere, Dive, Pussard & Vickerman, 1984 were identified based on morphological data and molecular genetic research methods. All these species of amoebae are characterized according to our own observations and measurements and the published data. The amoebae we found belong to three classes (Tubulinea, Discosea, Heterolobosea), six orders (Euamoebida, Thecamoebida, Dactylopodida, Vannellida, Centramoebida, Schizopyrenida), six families (Hartmannellidae, Thecamoebidae, Vexilliferidae, Vannellidae, Acanthamoebidae, Vahlkampfiidae) and six genera (Saccamoeba, Thecamoeba, Vexillifera, Vannella, Acanthamoeba, Willaertia). W. magna was the least common in water bodies of different regions of Ukraine; Saccamoeba sp., V. bacillipedes in the studied water bodies are either uncommon species or occupy the middle position in terms of frequency of occurrence In the water bodies of Ukraine T. similis, V. lata, Acanthamoeba sp. are both the least common and the most common species, or occupy the middle position in terms of frequency of occurrence. The following ecological groups of naked amoebae have been identified in relation to temperature: eurythermal, psychrophilic stenothermal, thermophilic stenothermal. In relation to the concentration of dissolved oxygen in water: euryoxidic and stenooxidic. Three species of amoebae can withstand either a wide range of values of organic substances dissolved in water, or belong to stenobiont species. The recorded amoebae belong to monotactic (Saccamoeba sp.), striate (T. similis), dactylopodial (V. bacillipedes), fan-shaped (V. lata), acanthopodial (Acanthamoeba sp.) and eruptive (W. magna) morphotypes.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"14 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79755511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Akramova, U. Shakarbaev, Z. Yorkulov, I. Arepbaev, A. Mirzaeva, D. Azimov
Schistosomatidae are an actively studied ecological group of trematodes. Their ability to cause various parasitic diseases in animals and humans makes them an interesting object of study for a number of research centres worldwide. One of the commonest species in this group is Bilharziella polonica (Kowalewsky, 1895), whose mature stages have been recorded in aquatic and semi-aquatic birds in Uzbekistan. Our research team established that the following birds were infected with mature trematodes B. polonica: Anas platyrhynchos (23%), A. crecca (18%), Podiceps ruficollis (11%), Ardea cinerea (14%) and one individual of Oxyura leucocephala. The highest infection rate was shown by the mallard A. platyrhynchos (23%) and common teal A. crecca (18%). The infection intensity ranged between 2 and 27 individuals. Research into various types of water bodies in Karakalpakstan identified 10 mollusc species – Lymnaeidae (4 species), Planorbidae (4 species) and Physidae (2 species). Cercariae morphologically similar to larvae of B. polonica were found in two species, Planorbis planorbis and P. tangitarensis. 6 chicks of domestic ducks were experimentally infected with those cercariae to track the life cycle of B. polonica in the organism of a definitive host. Helmintholological dissections showed that every duck was infected with B. polonica, which became mature 23–27 days after the infection. Eggs of B. polonica were recorded in the excrement of one of the birds 33–35 days after the infection. Based on field and experimental research, we identify the mollusc P. tangitarensis as a new intermediate host for B. polonica in Uzbekistan.
{"title":"Life cycle of Bilharziella polonica (Trematoda, Schistosomatidae) parasite of semi-aquatic birds in Uzbekistan","authors":"F. Akramova, U. Shakarbaev, Z. Yorkulov, I. Arepbaev, A. Mirzaeva, D. Azimov","doi":"10.15421/012213","DOIUrl":"https://doi.org/10.15421/012213","url":null,"abstract":"Schistosomatidae are an actively studied ecological group of trematodes. Their ability to cause various parasitic diseases in animals and humans makes them an interesting object of study for a number of research centres worldwide. One of the commonest species in this group is Bilharziella polonica (Kowalewsky, 1895), whose mature stages have been recorded in aquatic and semi-aquatic birds in Uzbekistan. Our research team established that the following birds were infected with mature trematodes B. polonica: Anas platyrhynchos (23%), A. crecca (18%), Podiceps ruficollis (11%), Ardea cinerea (14%) and one individual of Oxyura leucocephala. The highest infection rate was shown by the mallard A. platyrhynchos (23%) and common teal A. crecca (18%). The infection intensity ranged between 2 and 27 individuals. Research into various types of water bodies in Karakalpakstan identified 10 mollusc species – Lymnaeidae (4 species), Planorbidae (4 species) and Physidae (2 species). Cercariae morphologically similar to larvae of B. polonica were found in two species, Planorbis planorbis and P. tangitarensis. 6 chicks of domestic ducks were experimentally infected with those cercariae to track the life cycle of B. polonica in the organism of a definitive host. Helmintholological dissections showed that every duck was infected with B. polonica, which became mature 23–27 days after the infection. Eggs of B. polonica were recorded in the excrement of one of the birds 33–35 days after the infection. Based on field and experimental research, we identify the mollusc P. tangitarensis as a new intermediate host for B. polonica in Uzbekistan.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"26 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77502789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Yevstafieva, A. Khorolskyi, S. Kravchenko, V. Melnychuk, O. Nikiforova, O. Reshetylo
Passalurosis is a prevalent disease among helminthiases of domestic rabbits. This invasion is caused by the nematode Passalurus ambiguus (Nematoda, Oxyuroidae), which is cosmopolitan and localized in the cecum and colon of rabbits. Passalurosis is highly contagious and capable of unlimited spread, due to the biological characteristics of its pathogen, such as the conditions that ensure maximum preservation of parasites at exogenous stages of their development. Experimental research in the laboratory established the timing of development of P. ambiguus eggs isolated from the gonads of female helminths, depending on temperature regimes and features of their growth and development. According to the morphological features of Passalurus eggs, four stages were distinguished in their exogenous development: zygote, cleavage and formation of blastomeres, formation of larvae and motile larvae. Depending on the cultivation temperature, the duration of embryogenesis ranged from 4 to 9 days, and egg viability ranged from 59.3% to 72.7%. The most favourable temperature regime for the development of P. ambiguus eggs was the temperature of 35 °C, at which 72.7% of eggs with motile larvae were formed within 4 days. At this temperature, the zygote stage lasted for 1 day of cultivation, the stage of cleaving and formation of blastomeres occurred on days 1–2, the stage of larval formation on days 1–3, and the stage of formation of motile larva on days 3–4. At lower temperatures, the term of development of Passalurus eggs increased, and the number of viable eggs decreased. At temperatures of 30 °С and 25 °С, the development of Passalurus eggs took place during 5 and 7 days, respectively, and the viability was 66.7% and 62.7%. At these temperatures, the zygote stage lasted 1–2 and 1–3 days, the stage of cleaving and formation of blastomeres occurred on days 1–3 and 1–4, the stage of larval formation lasted from days 2–4 and 2–6, and the stage of formation of motile larvae took place on days 3–5 and 4–7, respectively. The least favourable temperature for the development of P. ambiguus eggs was the temperature of 20 °С, at which the formation of motile larvae occurred in 9 days, and their viability was only 59.3%. At this temperature, the zygote stage lasted 1–4 days, the stage of cleaving and blastomere formation occurred on days 2–6, and the larval formation stage on days 3–8, and the motile larval stage happened on days 5–9. The growth and development of P. ambiguus eggs was accompanied by significant changes in morphometric parameters, such as the increase in egg width and thinning of egg shell at the egg shell plug. The obtained data will allow preventive measures to be effectively implemented on rabbit farms that are susceptible to pinworms, taking into account the terms of exogenous development of pathogens in different seasons.
{"title":"Features of the exogenic development of Passalurus ambiguus (Nematoda, Oxyuroidae) at different temperature regimes","authors":"V. Yevstafieva, A. Khorolskyi, S. Kravchenko, V. Melnychuk, O. Nikiforova, O. Reshetylo","doi":"10.15421/012207","DOIUrl":"https://doi.org/10.15421/012207","url":null,"abstract":"Passalurosis is a prevalent disease among helminthiases of domestic rabbits. This invasion is caused by the nematode Passalurus ambiguus (Nematoda, Oxyuroidae), which is cosmopolitan and localized in the cecum and colon of rabbits. Passalurosis is highly contagious and capable of unlimited spread, due to the biological characteristics of its pathogen, such as the conditions that ensure maximum preservation of parasites at exogenous stages of their development. Experimental research in the laboratory established the timing of development of P. ambiguus eggs isolated from the gonads of female helminths, depending on temperature regimes and features of their growth and development. According to the morphological features of Passalurus eggs, four stages were distinguished in their exogenous development: zygote, cleavage and formation of blastomeres, formation of larvae and motile larvae. Depending on the cultivation temperature, the duration of embryogenesis ranged from 4 to 9 days, and egg viability ranged from 59.3% to 72.7%. The most favourable temperature regime for the development of P. ambiguus eggs was the temperature of 35 °C, at which 72.7% of eggs with motile larvae were formed within 4 days. At this temperature, the zygote stage lasted for 1 day of cultivation, the stage of cleaving and formation of blastomeres occurred on days 1–2, the stage of larval formation on days 1–3, and the stage of formation of motile larva on days 3–4. At lower temperatures, the term of development of Passalurus eggs increased, and the number of viable eggs decreased. At temperatures of 30 °С and 25 °С, the development of Passalurus eggs took place during 5 and 7 days, respectively, and the viability was 66.7% and 62.7%. At these temperatures, the zygote stage lasted 1–2 and 1–3 days, the stage of cleaving and formation of blastomeres occurred on days 1–3 and 1–4, the stage of larval formation lasted from days 2–4 and 2–6, and the stage of formation of motile larvae took place on days 3–5 and 4–7, respectively. The least favourable temperature for the development of P. ambiguus eggs was the temperature of 20 °С, at which the formation of motile larvae occurred in 9 days, and their viability was only 59.3%. At this temperature, the zygote stage lasted 1–4 days, the stage of cleaving and blastomere formation occurred on days 2–6, and the larval formation stage on days 3–8, and the motile larval stage happened on days 5–9. The growth and development of P. ambiguus eggs was accompanied by significant changes in morphometric parameters, such as the increase in egg width and thinning of egg shell at the egg shell plug. The obtained data will allow preventive measures to be effectively implemented on rabbit farms that are susceptible to pinworms, taking into account the terms of exogenous development of pathogens in different seasons.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"49 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90759526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Khromykh, Y. Lykholat, O. Didur, T. Sklyar, V. Davydov, K. Lavrentievа, T. Lykholat
Plants of two species of Actinidia genus grown in an adverse steppe climate were examined in terms of secondary metabolites’ accumulation, antioxidant potential, and antimicrobial ability. The aim of the work was to reveal whether the introduced plants A. arguta and A. polygama retain their well-known health benefits. Total content of polyphenols (549.2 and 428.1 mg GAE/100 g FW, respectively), flavonoids, and phenolic acids as well as total antioxidant activity and reducing power of the fruit isopropanol extracts were found to be equal or even higher than the reported data on kiwifruit varieties cultivated in China and other regions. Antioxidant potential and phenolic compounds’ content in the fruit peel of both species were higher when compared to pulp, while corresponding indices of leaves exceeded those of the fruit. Disc-diffusion assays showed low to moderate antibacterial activity of A. arguta and A. polygama fruit and leaf extracts against collection Gram-negative and Gram-positive strains. Clinical strains of P. aeruginosa and E. coli resistant to the action of ofloxacin were notably inhibited by A. arguta and A. polygama fruit and leaf crude extracts. Inhibiting effects of plant extracts on clinical strains of K. pneumoniae and A. baumannii were comparable with the effect of ofloxacin. GC-MS assays identified 23 and 36 chemical constituents, respectively in A. arguta and A. polygama fruit isopropanol extracts. The main compounds in both extracts were 2-propenoic acid, pentadecyl ester followed by squalene, 7,9-di-tert-butyl-1-oxaspiro(4,5)deca-6,9-dien-2,8-dione, octadecanoic acid, 2-oxo-methyl ester, ethyl-isoallocholate, and phytol having known bioactivities. Our findings confirmed the preservation of useful properties by the introduced plants and also indicated the rich health-promoting abilities and expedience of cultivating A. arguta and A. polygama in a steppe climate.
对生长在不利草原气候条件下的两种猕猴桃属植物的次生代谢产物积累、抗氧化潜能和抗菌能力进行了研究。这项工作的目的是揭示引进的植物A. arguta和A. polygama是否保留其众所周知的健康益处。果实异丙醇提取物的总多酚含量(分别为549.2和428.1 mg GAE/100 g FW)、总黄酮和酚酸含量以及总抗氧化活性和还原力均等于甚至高于中国和其他地区猕猴桃品种的报道数据。果皮的抗氧化电位和酚类化合物含量均高于果肉，而叶片的抗氧化电位和酚类化合物含量均高于果实。圆盘扩散试验结果表明，软枣仁和玉麻果叶提取物对革兰氏阴性和革兰氏阳性菌株的抑菌活性较低至中等。对氧氟沙星耐药的铜绿假单胞菌和大肠杆菌临床菌株均受软枣仁和玉麻果叶粗提物的显著抑制。植物提取物对肺炎克雷伯菌和鲍曼不动杆菌临床菌株的抑制作用与氧氟沙星相当。气相色谱-质谱分析分别鉴定了香枣和玉麻果异丙醇提取物中的23种和36种化学成分。两种提取物的主要成分均为2-丙烯酸、五烷基酯，其次为角鲨烯、7,9-二叔丁基-1-氧化螺(4,5)十二-6,9-二-2,8-二酮、十八烷酸、2-氧化甲酯、异胆酸乙酯和已知生物活性的叶绿醇。本研究结果证实了引种植物保留了植物的有益特性，同时也说明了在草原气候条件下栽培软叶麻和多叶麻具有丰富的促进健康的能力和方便性。
{"title":"Phytochemical profiles, antioxidant and antimicrobial activity of Actinidia polygama and A. arguta fruits and leaves","authors":"N. Khromykh, Y. Lykholat, O. Didur, T. Sklyar, V. Davydov, K. Lavrentievа, T. Lykholat","doi":"10.15421/012205","DOIUrl":"https://doi.org/10.15421/012205","url":null,"abstract":"Plants of two species of Actinidia genus grown in an adverse steppe climate were examined in terms of secondary metabolites’ accumulation, antioxidant potential, and antimicrobial ability. The aim of the work was to reveal whether the introduced plants A. arguta and A. polygama retain their well-known health benefits. Total content of polyphenols (549.2 and 428.1 mg GAE/100 g FW, respectively), flavonoids, and phenolic acids as well as total antioxidant activity and reducing power of the fruit isopropanol extracts were found to be equal or even higher than the reported data on kiwifruit varieties cultivated in China and other regions. Antioxidant potential and phenolic compounds’ content in the fruit peel of both species were higher when compared to pulp, while corresponding indices of leaves exceeded those of the fruit. Disc-diffusion assays showed low to moderate antibacterial activity of A. arguta and A. polygama fruit and leaf extracts against collection Gram-negative and Gram-positive strains. Clinical strains of P. aeruginosa and E. coli resistant to the action of ofloxacin were notably inhibited by A. arguta and A. polygama fruit and leaf crude extracts. Inhibiting effects of plant extracts on clinical strains of K. pneumoniae and A. baumannii were comparable with the effect of ofloxacin. GC-MS assays identified 23 and 36 chemical constituents, respectively in A. arguta and A. polygama fruit isopropanol extracts. The main compounds in both extracts were 2-propenoic acid, pentadecyl ester followed by squalene, 7,9-di-tert-butyl-1-oxaspiro(4,5)deca-6,9-dien-2,8-dione, octadecanoic acid, 2-oxo-methyl ester, ethyl-isoallocholate, and phytol having known bioactivities. Our findings confirmed the preservation of useful properties by the introduced plants and also indicated the rich health-promoting abilities and expedience of cultivating A. arguta and A. polygama in a steppe climate.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"130 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77445345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Sukhomlin, M. Zinchenko, O. Zinchenko, V. S. Tepliuk, Y. V. Biletskyi, V. Ivantsiv, M. G. Biletska, L. Buslenko, S. V. Budnik
The subgenus Boophthora is a typical Palearctic taxon, which includes only 6 species, among them. Simulium erythrocephalum has a transpalearctic distribution. In Europe, Adler notes only the species S. erythrocephalum, and Yankovsky – two species S. (Boophthora) erythrocephalum and S. (Boophthora) chelevini. According to morphological characteristics, these species differ in their life stages. We have studied the development of S. erythrocephalum and S. chelevini from three rivers of Volyn region, Ukraine (Styr, Chornoguzka, Putylivka) from 2017 to 2019. We used the EPPO PM7 / 129 standard. Collected samples, 615-bp fragments of the COI gene were sequenced from five individuals of S. erythrocephalum and five individuals of S. chelevini and compared with four samples of S. erythrocephalum from the GenBank. We obtained the nucleotide sequence of S. chelevini. All of the S. erythrocephalum samples from Ukraine had 692 bases, the S. erythrocephalum samples from Armenia had 673 bases. S. erythrocephalum and S. chelevini did not have any intraspecific variations. These intraspecific variations were not larger than the interspecific variations. It has been proved that the populations of S. erythrocephalum and S. chelevini from medium and small rivers of Volyn do not differ in biological, behavioural and genetic characteristics. Comparison of S. erythrocephalum and S. chelevini life stages showed clear differences in 20 morphological features, which are probably manifestations of phenotypic variability. Comparison of species with data from the GenBank from Spain and Armenia on the mitochondrial cytochrome c oxidase subunit I (COI) gene confirmed the opinion that S. erythrocephalum and S. chelevini are one species. On the phylogenetic tree, the data are not grouped, there is no clear separation of the clades. Bootstrap values are 95–100%, which may indicate a significant similarity of all studied samples and the lack of isolation of individual morphotypes from Volyn, Spain and Armenia. To finally confirm the taxonomic position of these two species, additional research is needed covering more individuals from different parts of Europe and analysis of more genes.
{"title":"The biology of Simulium erythrocephalum and S. chelevini (Diptera, Simuliidae): Morpho-logical, ecological and molecular data","authors":"K. Sukhomlin, M. Zinchenko, O. Zinchenko, V. S. Tepliuk, Y. V. Biletskyi, V. Ivantsiv, M. G. Biletska, L. Buslenko, S. V. Budnik","doi":"10.15421/012201","DOIUrl":"https://doi.org/10.15421/012201","url":null,"abstract":"The subgenus Boophthora is a typical Palearctic taxon, which includes only 6 species, among them. Simulium erythrocephalum has a transpalearctic distribution. In Europe, Adler notes only the species S. erythrocephalum, and Yankovsky – two species S. (Boophthora) erythrocephalum and S. (Boophthora) chelevini. According to morphological characteristics, these species differ in their life stages. We have studied the development of S. erythrocephalum and S. chelevini from three rivers of Volyn region, Ukraine (Styr, Chornoguzka, Putylivka) from 2017 to 2019. We used the EPPO PM7 / 129 standard. Collected samples, 615-bp fragments of the COI gene were sequenced from five individuals of S. erythrocephalum and five individuals of S. chelevini and compared with four samples of S. erythrocephalum from the GenBank. We obtained the nucleotide sequence of S. chelevini. All of the S. erythrocephalum samples from Ukraine had 692 bases, the S. erythrocephalum samples from Armenia had 673 bases. S. erythrocephalum and S. chelevini did not have any intraspecific variations. These intraspecific variations were not larger than the interspecific variations. It has been proved that the populations of S. erythrocephalum and S. chelevini from medium and small rivers of Volyn do not differ in biological, behavioural and genetic characteristics. Comparison of S. erythrocephalum and S. chelevini life stages showed clear differences in 20 morphological features, which are probably manifestations of phenotypic variability. Comparison of species with data from the GenBank from Spain and Armenia on the mitochondrial cytochrome c oxidase subunit I (COI) gene confirmed the opinion that S. erythrocephalum and S. chelevini are one species. On the phylogenetic tree, the data are not grouped, there is no clear separation of the clades. Bootstrap values are 95–100%, which may indicate a significant similarity of all studied samples and the lack of isolation of individual morphotypes from Volyn, Spain and Armenia. To finally confirm the taxonomic position of these two species, additional research is needed covering more individuals from different parts of Europe and analysis of more genes.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"75 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86046242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study of the longhorn beetles fauna in Ukraine has been going on for two centuries. During this time, huge collections of materials have been accumulated. These enabled a comprehensive view of the fauna of the longhorn beetles in Ukraine to be formed. However, despite this, the first complete list of the longhorn beetles of the fauna of Ukraine was compiled by Zahajkevych only in the late twentieth century. He listed 275 species. Decades later, Bartenev supplemented Zahajkevych’s list with five more species, suggesting that there are 280 species in Ukraine. In 2009, Bartenev expanded his previous list to 284 species. Recently, however, the fauna of the longhorn beetles in Ukraine has been fluctuating due to climate change. The southern species are actively expanding their range to the north, and seven new species of longhorn beetles have been identified in Ukraine over the past decade. In addition, the new synonymy is also the reason for changes in the list of the longhorn beetles in Ukraine. In particular, from Bartenev’s latest list, I removed 14 synonymous species and 5 species that have never been registered in Ukraine. In total, the list of the longhorn beetles I have revised includes 279 species from 114 genera, 44 tribes and 6 subfamilies. One of them, Batocera lineolata Chevrolat, 1852, is not naturalized in Ukraine and is known from the only record of a female reared from wooden packaging materials. The degree of study of the longhorn beetle fauna of the physiographic regions of Ukraine is very uneven. The fauna of the western, northern, eastern and extreme southern regions of Ukraine is the most fully studied. At the same time, the fauna of the central regions of Ukraine is still very poorly known. Further research on the longhorn beetles in Ukraine should be conducted in two directions: 1) completing the lists for physiographic regions and 2) monitoring fauna changes under the influence of climate change.
{"title":"The longhorn beetles (Coleoptera, Cerambycidae) of Ukraine: Results of two centuries of research","authors":"A. Zamoroka","doi":"10.15421/012206","DOIUrl":"https://doi.org/10.15421/012206","url":null,"abstract":"The study of the longhorn beetles fauna in Ukraine has been going on for two centuries. During this time, huge collections of materials have been accumulated. These enabled a comprehensive view of the fauna of the longhorn beetles in Ukraine to be formed. However, despite this, the first complete list of the longhorn beetles of the fauna of Ukraine was compiled by Zahajkevych only in the late twentieth century. He listed 275 species. Decades later, Bartenev supplemented Zahajkevych’s list with five more species, suggesting that there are 280 species in Ukraine. In 2009, Bartenev expanded his previous list to 284 species. Recently, however, the fauna of the longhorn beetles in Ukraine has been fluctuating due to climate change. The southern species are actively expanding their range to the north, and seven new species of longhorn beetles have been identified in Ukraine over the past decade. In addition, the new synonymy is also the reason for changes in the list of the longhorn beetles in Ukraine. In particular, from Bartenev’s latest list, I removed 14 synonymous species and 5 species that have never been registered in Ukraine. In total, the list of the longhorn beetles I have revised includes 279 species from 114 genera, 44 tribes and 6 subfamilies. One of them, Batocera lineolata Chevrolat, 1852, is not naturalized in Ukraine and is known from the only record of a female reared from wooden packaging materials. The degree of study of the longhorn beetle fauna of the physiographic regions of Ukraine is very uneven. The fauna of the western, northern, eastern and extreme southern regions of Ukraine is the most fully studied. At the same time, the fauna of the central regions of Ukraine is still very poorly known. Further research on the longhorn beetles in Ukraine should be conducted in two directions: 1) completing the lists for physiographic regions and 2) monitoring fauna changes under the influence of climate change.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"32 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85354484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Kots, L. Rybachenko, A. Khrapova, K. Kukol, O. R. Rybachenko, Y. O. Кhomenko
A distinctive feature of legumes is the ability to combine two most important processes: photosynthesis and nitrogen fixation. However, the course of those processes, and therefore seed potential of those crops depend on a number of biotic and abiotic factors, the commonest being drought. Therefore, interest in physical-biochemical resistance of the plant organism to abiotic stress factors is increasing, as well as search for optimum ways to increase its adaptability. Success of adaptation of a plant’s organism to unfavourable environmental factors is known to largely depend on optimal functioning of assimilative apparatus. Some indicators of the condition of the apparatus are the content and ratio of photosynthesis pigments. Therefore, we aimed at determining the reaction of the pigment complex of Glycine max (L.) Merr. plants, grown against the background of optimal and insufficient watering, to inoculation of seeds with rhizobia bacteria Bradyrhizobium japonicum, cultivated using nanocarboxylates of chromium, cobalt, iron, copper and germanium. Research has shown that utilization of germanium nanocarboxylate as a component of inoculative suspension led to the highest content of chlorophylls in leaves of soybean of the studied variants in the blossoming phase during optimal watering, as well as significant increase in the content of carotenoids compared with the control plants regardless of the level of watering. At the same time, this element caused no significant effect on the chlorophyll content in plants grown in drought. It was confirmed that among soybean plants that were in stress conditions (blossoming phase) for two weeks, the highest content of chlorophylls was in leaves of plants grown from seeds inoculated with rhizobial suspension with addition of chromium and copper nanocarboxylates, which caused 25.3% and 22.8% increase in chlorophyll а, 29.4% and 32.3% in chlorophyll b and 26.4%% and 23.8% in them respectively, compared with the control. Furthermore, chromium and copper nanocarboxylates stimulated the content of carotenoids in the same plants, though it was less expressed than after adding germanium nanocarboxylate. The highest content of photosynthetic pigments in plants after the watering was resumed (phase of bean formation) was in cases of applying chromium and germanium nanocarboxylates. It was confirmed that the most efficient way to protect the pigment complex of soybean plants during drought was using chromium and germanium nanocarboxylates as components of inoculation suspension. The results we obtained indicate the possibility of applying chromium nanocarboxylate in the technology of cultivating soybean in the conditions of water deficiency as an effective way to improve biosynthesis of chlorophylls, as well as using germanium nanocarboxyllate as a component that provides a high level of activity of protective mechanisms of the pigment system of soybean, associated with resisting stress caused by water deficiency.
{"title":"Composition of pigment complex in leaves of soybean plants, inoculated by Bradyrhizobium japonicum, subject to metal nanocarboxylates and various-levels of water supply","authors":"S. Kots, L. Rybachenko, A. Khrapova, K. Kukol, O. R. Rybachenko, Y. O. Кhomenko","doi":"10.15421/012208","DOIUrl":"https://doi.org/10.15421/012208","url":null,"abstract":"A distinctive feature of legumes is the ability to combine two most important processes: photosynthesis and nitrogen fixation. However, the course of those processes, and therefore seed potential of those crops depend on a number of biotic and abiotic factors, the commonest being drought. Therefore, interest in physical-biochemical resistance of the plant organism to abiotic stress factors is increasing, as well as search for optimum ways to increase its adaptability. Success of adaptation of a plant’s organism to unfavourable environmental factors is known to largely depend on optimal functioning of assimilative apparatus. Some indicators of the condition of the apparatus are the content and ratio of photosynthesis pigments. Therefore, we aimed at determining the reaction of the pigment complex of Glycine max (L.) Merr. plants, grown against the background of optimal and insufficient watering, to inoculation of seeds with rhizobia bacteria Bradyrhizobium japonicum, cultivated using nanocarboxylates of chromium, cobalt, iron, copper and germanium. Research has shown that utilization of germanium nanocarboxylate as a component of inoculative suspension led to the highest content of chlorophylls in leaves of soybean of the studied variants in the blossoming phase during optimal watering, as well as significant increase in the content of carotenoids compared with the control plants regardless of the level of watering. At the same time, this element caused no significant effect on the chlorophyll content in plants grown in drought. It was confirmed that among soybean plants that were in stress conditions (blossoming phase) for two weeks, the highest content of chlorophylls was in leaves of plants grown from seeds inoculated with rhizobial suspension with addition of chromium and copper nanocarboxylates, which caused 25.3% and 22.8% increase in chlorophyll а, 29.4% and 32.3% in chlorophyll b and 26.4%% and 23.8% in them respectively, compared with the control. Furthermore, chromium and copper nanocarboxylates stimulated the content of carotenoids in the same plants, though it was less expressed than after adding germanium nanocarboxylate. The highest content of photosynthetic pigments in plants after the watering was resumed (phase of bean formation) was in cases of applying chromium and germanium nanocarboxylates. It was confirmed that the most efficient way to protect the pigment complex of soybean plants during drought was using chromium and germanium nanocarboxylates as components of inoculation suspension. The results we obtained indicate the possibility of applying chromium nanocarboxylate in the technology of cultivating soybean in the conditions of water deficiency as an effective way to improve biosynthesis of chlorophylls, as well as using germanium nanocarboxyllate as a component that provides a high level of activity of protective mechanisms of the pigment system of soybean, associated with resisting stress caused by water deficiency.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"28 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84758260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. I. Babytskiy, O. O. Bezsmertna, Y. V. Protsenko, S. D. Pavliuk, N. V. Rubanovska
Sciarids (Diptera, Sciaridae) or black fungus gnats are small, mainly dark coloured insects whose larvae usually develop in rotting plant remains permeated by fungal hyphae. Typical habitats for sciarids are shaded forests and wet meadows, but some species can migrate from natural biotopes to anthropogenic ecosystems and live as synanthropes. We have investigated ecological and chorological features of sciarids in Ukraine since 2012. Within this work, we collected imagoes during expeditions and excursions in different biotopes using the Malaise trap, by the method of sweeping and with exhauster directly from substrate. Collected imagoes were placed into 5 mL vials with 70% ethanol. In the lab fixed material was dehydrated in absolute ethanol and mounted on the slides in Euparal. Previous registrations are based on 6 field collections, two of them were carried out in the XIX century, 4 observations of “army worms” and two pest records. From published material of these collections 78 sciarid species were known from 17 genera in 8 more or less specified localities of Ukraine. Our sciarid study in the country expanded the data on registered sciarids by adding 18 new species and enriched information about the distribution of sciarids by 86 new findings of previously known species in 12 regions (Volyn, Cherkasy, Chernihiv, Ivano-Frankivsk, Kharkiv, Khmelnytsk, Kyiv, Lviv, Odesa, Poltava, Rivne and Ternopil) of Ukraine. The current checklist of Sciaridae of Ukraine contains 96 species from 17 genera in 168 localities. Through comparison with Germany, whose sciarid fauna is studied much better, for Ukraine we can predict the presence approximately 400 sciarid species, so the species diversity of Sciaridae in Ukraine still poorly known for the known species does not exceed 25 percent of presumed existing species here. The majority of sciarid findings are registered in different types of broadleaf forest biotopes, only a few species have been found also in grassland habitats. Some sciarid species show synanthropic attraction and have been registered in anthropogenic habitats, for example Bradysia fenestralis (pest of cultivated plants in greenhouses), Corynoptera dentiforceps, Scatopsciara atomaria and Corynoptera tridentata. Larvae of Bradysia placida develop in rotten wood, also development of Corynoptera membranigera preimaginal phases possibly takes place in the fruit bodies of fungi (Neoboletus luridiformis, Russula sp.) and Mycetozoa (Fuligo septica).
{"title":"Biodiversity of Sciaridae (Diptera) in Ukraine","authors":"A. I. Babytskiy, O. O. Bezsmertna, Y. V. Protsenko, S. D. Pavliuk, N. V. Rubanovska","doi":"10.15421/012202","DOIUrl":"https://doi.org/10.15421/012202","url":null,"abstract":"Sciarids (Diptera, Sciaridae) or black fungus gnats are small, mainly dark coloured insects whose larvae usually develop in rotting plant remains permeated by fungal hyphae. Typical habitats for sciarids are shaded forests and wet meadows, but some species can migrate from natural biotopes to anthropogenic ecosystems and live as synanthropes. We have investigated ecological and chorological features of sciarids in Ukraine since 2012. Within this work, we collected imagoes during expeditions and excursions in different biotopes using the Malaise trap, by the method of sweeping and with exhauster directly from substrate. Collected imagoes were placed into 5 mL vials with 70% ethanol. In the lab fixed material was dehydrated in absolute ethanol and mounted on the slides in Euparal. Previous registrations are based on 6 field collections, two of them were carried out in the XIX century, 4 observations of “army worms” and two pest records. From published material of these collections 78 sciarid species were known from 17 genera in 8 more or less specified localities of Ukraine. Our sciarid study in the country expanded the data on registered sciarids by adding 18 new species and enriched information about the distribution of sciarids by 86 new findings of previously known species in 12 regions (Volyn, Cherkasy, Chernihiv, Ivano-Frankivsk, Kharkiv, Khmelnytsk, Kyiv, Lviv, Odesa, Poltava, Rivne and Ternopil) of Ukraine. The current checklist of Sciaridae of Ukraine contains 96 species from 17 genera in 168 localities. Through comparison with Germany, whose sciarid fauna is studied much better, for Ukraine we can predict the presence approximately 400 sciarid species, so the species diversity of Sciaridae in Ukraine still poorly known for the known species does not exceed 25 percent of presumed existing species here. The majority of sciarid findings are registered in different types of broadleaf forest biotopes, only a few species have been found also in grassland habitats. Some sciarid species show synanthropic attraction and have been registered in anthropogenic habitats, for example Bradysia fenestralis (pest of cultivated plants in greenhouses), Corynoptera dentiforceps, Scatopsciara atomaria and Corynoptera tridentata. Larvae of Bradysia placida develop in rotten wood, also development of Corynoptera membranigera preimaginal phases possibly takes place in the fruit bodies of fungi (Neoboletus luridiformis, Russula sp.) and Mycetozoa (Fuligo septica).","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"2 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83790434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hnatush, O. Maslovska, S. Komplikevych, I. V. Kovbasa
Heavy metals that enter the environment due to natural processes or industrial activities, when accumulated, have a negative impact on organisms, including microorganisms. Microorganisms have developed various adaptations to heavy metal compounds. The aim of our work was to investigate the influence of ferric citrate and cobalt (II) chloride on biomass accumulation, indicators of free radical damage and activity of enzymes of the antioxidant defense system of bacteria Rhodopseudomonas yavorovii IMV B-7620, that were isolated from the water of Yavorivske Lake (Ukraine, Lviv region), which was formed as a result of flooding of a sulfur quarry. We used cultural, photometric methods, and statistical processing of the results was performed using two-way ANOVA and factor analysis. It was found that ferric citrate at a concentration of 1–12 mM causes inhibition of the accumulation of biomass of bacteria Rh. yavorovii IMV B-7620 up to 44.7%, and cobalt (II) chloride at a concentration of 1–15 mM – up to 70.4%, compared with the control. The studied concentrations of ferric citrate and cobalt (II) chloride cause free radical damage to lipids and proteins of Rh. yavorovii IMV B-7620. As a result of two-way ANOVA we found that under the influence of ferric citrate statistically significant changes in biomass accumulation, lipid hydroperoxides and thiobarbiturate reactive species content, superoxide dismutase activity were predetermined by increasing the concentration of metal salts as well as increasing the duration of cultivation of bacteria, while the content of diene conjugates and catalase activity changed with increasing duration of cultivation. Under the influence of cobalt (II) chloride, statistically significant changes in all studied indicators were found both due to the increase in the concentration of metal salts and with increasing duration of bacterial cultivation. The studied parameters of Rh. yavorovii IMV B-7620 cells under the influence of ferric citrate and cobalt (II) chloride are combined into two factors, that explain 95.4% and 99.2% of the total data variance, respectively. Under the influence of ferric citrate, the first latent factor included diene conjugates, thiobarbiturate reactive species, carbonyl groups in proteins, which are closely linked by a direct bond and inversely related to the content of lipid hydroperoxides and catalase activity. The second latent factor included duration of cultivation of bacteria, biomass accumulation, and superoxide dismutase activity, which are inversely related to lipid hydroperoxide content and catalase activity. Under the influence of cobalt (II) chloride, the first latent factor included the content of lipid hydroperoxides, carbonyl groups in proteins, as well as catalase and superoxide dismutase activities, which are inversely related to bacterial biomass.
{"title":"Influence of cobalt chloride and ferric citrate on purple non-sulfur bacteria Rhodopseudomonas yavorovii","authors":"S. Hnatush, O. Maslovska, S. Komplikevych, I. V. Kovbasa","doi":"10.15421/012204","DOIUrl":"https://doi.org/10.15421/012204","url":null,"abstract":"Heavy metals that enter the environment due to natural processes or industrial activities, when accumulated, have a negative impact on organisms, including microorganisms. Microorganisms have developed various adaptations to heavy metal compounds. The aim of our work was to investigate the influence of ferric citrate and cobalt (II) chloride on biomass accumulation, indicators of free radical damage and activity of enzymes of the antioxidant defense system of bacteria Rhodopseudomonas yavorovii IMV B-7620, that were isolated from the water of Yavorivske Lake (Ukraine, Lviv region), which was formed as a result of flooding of a sulfur quarry. We used cultural, photometric methods, and statistical processing of the results was performed using two-way ANOVA and factor analysis. It was found that ferric citrate at a concentration of 1–12 mM causes inhibition of the accumulation of biomass of bacteria Rh. yavorovii IMV B-7620 up to 44.7%, and cobalt (II) chloride at a concentration of 1–15 mM – up to 70.4%, compared with the control. The studied concentrations of ferric citrate and cobalt (II) chloride cause free radical damage to lipids and proteins of Rh. yavorovii IMV B-7620. As a result of two-way ANOVA we found that under the influence of ferric citrate statistically significant changes in biomass accumulation, lipid hydroperoxides and thiobarbiturate reactive species content, superoxide dismutase activity were predetermined by increasing the concentration of metal salts as well as increasing the duration of cultivation of bacteria, while the content of diene conjugates and catalase activity changed with increasing duration of cultivation. Under the influence of cobalt (II) chloride, statistically significant changes in all studied indicators were found both due to the increase in the concentration of metal salts and with increasing duration of bacterial cultivation. The studied parameters of Rh. yavorovii IMV B-7620 cells under the influence of ferric citrate and cobalt (II) chloride are combined into two factors, that explain 95.4% and 99.2% of the total data variance, respectively. Under the influence of ferric citrate, the first latent factor included diene conjugates, thiobarbiturate reactive species, carbonyl groups in proteins, which are closely linked by a direct bond and inversely related to the content of lipid hydroperoxides and catalase activity. The second latent factor included duration of cultivation of bacteria, biomass accumulation, and superoxide dismutase activity, which are inversely related to lipid hydroperoxide content and catalase activity. Under the influence of cobalt (II) chloride, the first latent factor included the content of lipid hydroperoxides, carbonyl groups in proteins, as well as catalase and superoxide dismutase activities, which are inversely related to bacterial biomass.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"25 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78617096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：