Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749821
Jinye Xu, Jinping Cheng, S. Cheng, B. Chan
We present the successful fabrication of bovine serum albumin matrix with novel two-photon photochemical crosslinking technique. Electron scanning microscope images revealed the highly porous microstructures of as-fabricated matrices. Dose dependence of the microstructures on both BSA and Rose Bengal has been studied. 2D patterns and 3D micropillars of BSA have also been fabricated, which potentially can be used as a model of cell-matrix study.
{"title":"Submicron-fabrication of bovine serum albumin (BSA) matrix via two-photon photochemical crosslinking","authors":"Jinye Xu, Jinping Cheng, S. Cheng, B. Chan","doi":"10.1109/NANOMED.2010.5749821","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749821","url":null,"abstract":"We present the successful fabrication of bovine serum albumin matrix with novel two-photon photochemical crosslinking technique. Electron scanning microscope images revealed the highly porous microstructures of as-fabricated matrices. Dose dependence of the microstructures on both BSA and Rose Bengal has been studied. 2D patterns and 3D micropillars of BSA have also been fabricated, which potentially can be used as a model of cell-matrix study.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132980284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749822
Xueqiu You, J. Pak, J. Chang
This paper presents rapidly dissolving fibroin microneedles for the first time. A reverse PDMS microneedles mold was first created and drug-contained fibroin solution was poured into this reverse PDMS microneedles mold. Fibroin microneedles were successfully fabricated after drying and detaching the solidified fibroin structure from the PDMS mold. These fibroin microneedles serve as a matrix to incorporate drug molecules while maintaining the drug activity. The dimensions of the fabricated fibroin microneedles are 500 μm in length, 200 μm in diameter at the base, and 5 μm in radius at the tip. These fibroin matrix microneedles can dissolve within one minute under the skin to release the drug molecules and the dissolved fibroin in the skin generates noninflammatory amino acid degradation products usable in cell metabolic functions. The fibroin microneedles containing methylene blue as a drug were fabricated and their surface morphology, internal layered structure, mechanical property, and the dissolving characteristics were examined. These rapidly dissolving fibroin microneedles provide more benefit than conventional syringes for painless transdermal drug delivery.
{"title":"Rapidly dissolving silk protein microneedles for transdermal drug delivery","authors":"Xueqiu You, J. Pak, J. Chang","doi":"10.1109/NANOMED.2010.5749822","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749822","url":null,"abstract":"This paper presents rapidly dissolving fibroin microneedles for the first time. A reverse PDMS microneedles mold was first created and drug-contained fibroin solution was poured into this reverse PDMS microneedles mold. Fibroin microneedles were successfully fabricated after drying and detaching the solidified fibroin structure from the PDMS mold. These fibroin microneedles serve as a matrix to incorporate drug molecules while maintaining the drug activity. The dimensions of the fabricated fibroin microneedles are 500 μm in length, 200 μm in diameter at the base, and 5 μm in radius at the tip. These fibroin matrix microneedles can dissolve within one minute under the skin to release the drug molecules and the dissolved fibroin in the skin generates noninflammatory amino acid degradation products usable in cell metabolic functions. The fibroin microneedles containing methylene blue as a drug were fabricated and their surface morphology, internal layered structure, mechanical property, and the dissolving characteristics were examined. These rapidly dissolving fibroin microneedles provide more benefit than conventional syringes for painless transdermal drug delivery.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133809873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749838
S. Park, Jiyeon Kim, Y. Tak, J. Song
With the advent of new technologies, human exposure to ionizing radiation has increased. Therefore, appropriate pharmacological interventions and modalities are needed to protect humans against the deleterious effects of ionizing radiation. In this work, we developed a photosensitive, high-throughput chip-based assay for exploring the antioxidant [superoxide dismutase (SOD)]/radioprotective potential of herbal plants. Red light absorption property of nitroblue tetrazolium (NBT) formazan was applied to chip-based SOD activity measurements of six herbal plant extracts in a high-throughput manner. SOD enzyme in the photochemical reaction scavenged free radicals (O•−2) to form a stable carryover product (O2 and H2O2). This in turn inhibited the development of NBT formazan in the reaction. Thus, the inhibition of NBT formazan production in reaction samples compared to their controls provides for measuresing the SOD activity of the respective samples. All herbal plant extracts showed higher SOD activities than amifostine. Pueraria root and scutellaria root had higher SOD activities whereas the apricot kernel displayed the lowest SOD activity among the herbal plant samples. In all test samples, gamma ray irradiation mildly reduced SOD activity. However, the reduction in SOD activity between 5 and 20 Gy irradiated samples was relatively remarkable for ponicirus fruit and citrus unshiu peel. The results indicate that the tested herbal plant extracts have the potential to be used as radioprotectors. Among the tested herbal extracts, pueraria root showed the highest antioxidant/radioprotective activity and can be considered as preferred radioprotector candidate.
{"title":"On chip superoxide dismutase assay for high-throughput screening of radioprotective activity of herbal plants","authors":"S. Park, Jiyeon Kim, Y. Tak, J. Song","doi":"10.1109/NANOMED.2010.5749838","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749838","url":null,"abstract":"With the advent of new technologies, human exposure to ionizing radiation has increased. Therefore, appropriate pharmacological interventions and modalities are needed to protect humans against the deleterious effects of ionizing radiation. In this work, we developed a photosensitive, high-throughput chip-based assay for exploring the antioxidant [superoxide dismutase (SOD)]/radioprotective potential of herbal plants. Red light absorption property of nitroblue tetrazolium (NBT) formazan was applied to chip-based SOD activity measurements of six herbal plant extracts in a high-throughput manner. SOD enzyme in the photochemical reaction scavenged free radicals (O•−2) to form a stable carryover product (O2 and H2O2). This in turn inhibited the development of NBT formazan in the reaction. Thus, the inhibition of NBT formazan production in reaction samples compared to their controls provides for measuresing the SOD activity of the respective samples. All herbal plant extracts showed higher SOD activities than amifostine. Pueraria root and scutellaria root had higher SOD activities whereas the apricot kernel displayed the lowest SOD activity among the herbal plant samples. In all test samples, gamma ray irradiation mildly reduced SOD activity. However, the reduction in SOD activity between 5 and 20 Gy irradiated samples was relatively remarkable for ponicirus fruit and citrus unshiu peel. The results indicate that the tested herbal plant extracts have the potential to be used as radioprotectors. Among the tested herbal extracts, pueraria root showed the highest antioxidant/radioprotective activity and can be considered as preferred radioprotector candidate.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115167562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749841
H. Diao, C. Yu, B. Chan
The aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2–3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2–6 OFSCs into collagen type I microspheres, followed by a well-established cell migration assay under attraction of two kinds of chemokines −10 ng/ml Fractalkine or 50 ng/ml SDF-1β. After staining and counting, we found that after 3D collagen barrier selection, migrated OFSCs have more sensitive response to the induction of these two chemokines.
{"title":"Migratory response of human orbital fat-derived mesenchymal stem cell encapsulated in collagen gel","authors":"H. Diao, C. Yu, B. Chan","doi":"10.1109/NANOMED.2010.5749841","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749841","url":null,"abstract":"The aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2–3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2–6 OFSCs into collagen type I microspheres, followed by a well-established cell migration assay under attraction of two kinds of chemokines −10 ng/ml Fractalkine or 50 ng/ml SDF-1β. After staining and counting, we found that after 3D collagen barrier selection, migrated OFSCs have more sensitive response to the induction of these two chemokines.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133920152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749804
X. Fang, M. Tse, O. Tan, L. Sun
In this investigation, we present a novel label-free immunosensor platform based on inorganic barium strontium titanate (BST) thin film and interdigitated microelectrodes for clinical point-of-care application. The immunosensor is based on non-faradaic process, using DI water as electrolyte. As proof of concept, anti-human IgG, a common biomarker found and associated with many diseases was immobilized on the chemically modified BST film surface as sensing probe for human IgG detection. The BST immunosensor was also used for real clinical sample with pre-inactivated dengue virus as sensing probe to detect dengue antibody in human serum. Good linear correlations were observed for the measured electrical signals, AC impedances or current changes, versus the dilution of the human IgG concentration or the dengue serum samples. The measured signal changes in impedance/current without/with the presence of antibody were attributed to the surface conductivity change upon biomolecules immobilization and the dipole-induced interfacial polarization potential at the BST film/biomolecules interface. Promising specificity was proven with the interference tests by mixing human IgG solution with BSA and rabbit IgG. The performance of BST immunosensors exhibited good long-term stability with very low fluctuation/degradation even after storing dry at 4 °C for 60 days.
{"title":"A novel immunosensor platform based on inorganic BST film for point-of-care application","authors":"X. Fang, M. Tse, O. Tan, L. Sun","doi":"10.1109/NANOMED.2010.5749804","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749804","url":null,"abstract":"In this investigation, we present a novel label-free immunosensor platform based on inorganic barium strontium titanate (BST) thin film and interdigitated microelectrodes for clinical point-of-care application. The immunosensor is based on non-faradaic process, using DI water as electrolyte. As proof of concept, anti-human IgG, a common biomarker found and associated with many diseases was immobilized on the chemically modified BST film surface as sensing probe for human IgG detection. The BST immunosensor was also used for real clinical sample with pre-inactivated dengue virus as sensing probe to detect dengue antibody in human serum. Good linear correlations were observed for the measured electrical signals, AC impedances or current changes, versus the dilution of the human IgG concentration or the dengue serum samples. The measured signal changes in impedance/current without/with the presence of antibody were attributed to the surface conductivity change upon biomolecules immobilization and the dipole-induced interfacial polarization potential at the BST film/biomolecules interface. Promising specificity was proven with the interference tests by mixing human IgG solution with BSA and rabbit IgG. The performance of BST immunosensors exhibited good long-term stability with very low fluctuation/degradation even after storing dry at 4 °C for 60 days.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126070351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749797
M. Han, Yan Liang Zhang, M. Y. Yu, C. Shee, W. T. Ang
Biological cells possess biochemical modules and physical shapes to maintain appropriate biological function. Different types of force and deformation are applied on cells to investigate the response and mechanical properties. In the biophysics field, studies use indentation deformation on cell membranes to examine the elastic-viscoelastic properties of biological cells. Experiments in different predefined profiles and frequencies are required to test the fidelity and predictive capability of cells creep function. The accuracy and the repeatability of the given stimulus are the significant factor in the experiments to obtain reliable measurements, which are very difficult to realize using manual operations. Automatic micromanipulation systems have substantial advantages over the conventional manual operations in aspects of reliability, accuracy and repeatability. In this paper, an automatic micromanipulation system is introduced and a series of experiments are conducted to stress zebrafish embryo in different sinusoidal profiles. The experimental results show that the system is able to stress the biological cell in desired stimulation and give consistent force outputs in realtime, meanwhile mechanical properties of the zebrafish embryo are also analyzed.
{"title":"Real-time stressing and force sensing on biological cells","authors":"M. Han, Yan Liang Zhang, M. Y. Yu, C. Shee, W. T. Ang","doi":"10.1109/NANOMED.2010.5749797","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749797","url":null,"abstract":"Biological cells possess biochemical modules and physical shapes to maintain appropriate biological function. Different types of force and deformation are applied on cells to investigate the response and mechanical properties. In the biophysics field, studies use indentation deformation on cell membranes to examine the elastic-viscoelastic properties of biological cells. Experiments in different predefined profiles and frequencies are required to test the fidelity and predictive capability of cells creep function. The accuracy and the repeatability of the given stimulus are the significant factor in the experiments to obtain reliable measurements, which are very difficult to realize using manual operations. Automatic micromanipulation systems have substantial advantages over the conventional manual operations in aspects of reliability, accuracy and repeatability. In this paper, an automatic micromanipulation system is introduced and a series of experiments are conducted to stress zebrafish embryo in different sinusoidal profiles. The experimental results show that the system is able to stress the biological cell in desired stimulation and give consistent force outputs in realtime, meanwhile mechanical properties of the zebrafish embryo are also analyzed.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124958498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749823
S. Pané, O. Ergeneman, K. M. Sivaraman, T. Luhmann, H. Hall, B. Nelson
We present the state-of-the-art and future strategies for magnetic materials and functional coatings for biomédical microrobots. Coatings for sensing chemicals and for drug-delivery as well as ways of implementing them are being investigated. Two examples related to in traocular microrob ots are shown in this work: a luminescence p olymer-dye comp osite coatin g for th e detection of intraocular oxygen; and a conductive polymer coating for drug delivery purposes.
{"title":"Strategies for drug-delivery and chemical sensing using biomédical microrobots","authors":"S. Pané, O. Ergeneman, K. M. Sivaraman, T. Luhmann, H. Hall, B. Nelson","doi":"10.1109/NANOMED.2010.5749823","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749823","url":null,"abstract":"We present the state-of-the-art and future strategies for magnetic materials and functional coatings for biomédical microrobots. Coatings for sensing chemicals and for drug-delivery as well as ways of implementing them are being investigated. Two examples related to in traocular microrob ots are shown in this work: a luminescence p olymer-dye comp osite coatin g for th e detection of intraocular oxygen; and a conductive polymer coating for drug delivery purposes.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124771653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749799
Leyla Esfandiari, W. Tang
The use of MEMS to study the effect of mechanical compression on neurogenesis has been demonstrated. Polydimethylsiloxane — (PDMS)-based stretchable platforms were used on neurosphere assay to investigate the role of mechanical forces on the formation of radial glial processes, neuronal generation and migration. To induce mechanical compression on neurospheres, The PDMS culturing substrate is patterned with micron-sized wells. Neurospheres are cultured on the prestretched device. After 48 hours, when neurospheres are grown to the size of the well's width, the stretched substrate is released. The experimental results showed that applied mechanical compression on neural stem cells cultured as neurospheres could be a factor accelerating the radial glial formation, which is associated to neurogenesis and neuronal migration.
{"title":"PDMS stretchable platforms for the studies of mechanical compression on neurogenesis","authors":"Leyla Esfandiari, W. Tang","doi":"10.1109/NANOMED.2010.5749799","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749799","url":null,"abstract":"The use of MEMS to study the effect of mechanical compression on neurogenesis has been demonstrated. Polydimethylsiloxane — (PDMS)-based stretchable platforms were used on neurosphere assay to investigate the role of mechanical forces on the formation of radial glial processes, neuronal generation and migration. To induce mechanical compression on neurospheres, The PDMS culturing substrate is patterned with micron-sized wells. Neurospheres are cultured on the prestretched device. After 48 hours, when neurospheres are grown to the size of the well's width, the stretched substrate is released. The experimental results showed that applied mechanical compression on neural stem cells cultured as neurospheres could be a factor accelerating the radial glial formation, which is associated to neurogenesis and neuronal migration.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128017305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749837
Ronghui Wang, Yanbin Li, X. Mao, T. Huang, Huaguang Lu
A novel impedance biosensor was developed based on the combination of a bio-nanobead separation/concentration procedure and an interdigitated array nanoeletrode and was demonstrated for sensitive and rapid detection of H5 subtype of avian influenza virus (AIV). Magnetic nanobeads with a diameter of 30 nm were coated with H5 subtype-specific monoclonal antibodies to selectively capture the target virus. An interdigitated array nanoeletrode was designed and fabricated for impedance measurement. Changes in the impedance of the antibody coated nanobead-virus complex was measured and correlated to the presence of H5 AIV (e.g., H5N1). The nanobead and nanoeletrode based impedance biosensor was able to detect AIV H5N1 at titer of 0.0128 HA unit/50 μl. Equivalent circuit analysis indicated that the solution resistance was responsible for the impedance change due to the presence of target virus.
{"title":"Magnetic bio-nanobeads and nanoelectrode based impedance biosensor for detection of avian influenza virus","authors":"Ronghui Wang, Yanbin Li, X. Mao, T. Huang, Huaguang Lu","doi":"10.1109/NANOMED.2010.5749837","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749837","url":null,"abstract":"A novel impedance biosensor was developed based on the combination of a bio-nanobead separation/concentration procedure and an interdigitated array nanoeletrode and was demonstrated for sensitive and rapid detection of H5 subtype of avian influenza virus (AIV). Magnetic nanobeads with a diameter of 30 nm were coated with H5 subtype-specific monoclonal antibodies to selectively capture the target virus. An interdigitated array nanoeletrode was designed and fabricated for impedance measurement. Changes in the impedance of the antibody coated nanobead-virus complex was measured and correlated to the presence of H5 AIV (e.g., H5N1). The nanobead and nanoeletrode based impedance biosensor was able to detect AIV H5N1 at titer of 0.0128 HA unit/50 μl. Equivalent circuit analysis indicated that the solution resistance was responsible for the impedance change due to the presence of target virus.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129550842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/NANOMED.2010.5749831
Zhuxin Dong, U. Wejinya, Yanxia Zhu, K. Ye
The structure and properties of scaffold are important in cell-based tissue engineering, especially the mechanical property. Here, we quantify the dynamic oscillatory mechanical behavior of two kinds of porous collagen/chitosan scaffolds. The Young's Modulus were measured in PBS using Atomic Force Microscopy (AFM)-based nano-indentation in response to an imposed oscillatory deformation as a function of force, which can be converted to Young's Modulus. Collagen/chitosan scaffolds with different ratio (8:2 and 7:3, V/V), which already showed good properties for cell culture, were tested. The Young's Modulus of collagen/chitosan scaffold with ratio 7:3 is bigger than that of 8:2, which is consistent with our expectation. Force curves were obtained first from indentation, and then Young's Modulus was determined using a proper Hertz contact mathematical model. Meanwhile, the mechanical properties of mice pancreas and heart were obtained as controls. The results indicated that AFM-based nano-indentation is a good method for the mechanical property testing of porous scaffold.
{"title":"Force measurement study of engineered collagen-chitosan scaffold using Atomic Force Microscopy","authors":"Zhuxin Dong, U. Wejinya, Yanxia Zhu, K. Ye","doi":"10.1109/NANOMED.2010.5749831","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749831","url":null,"abstract":"The structure and properties of scaffold are important in cell-based tissue engineering, especially the mechanical property. Here, we quantify the dynamic oscillatory mechanical behavior of two kinds of porous collagen/chitosan scaffolds. The Young's Modulus were measured in PBS using Atomic Force Microscopy (AFM)-based nano-indentation in response to an imposed oscillatory deformation as a function of force, which can be converted to Young's Modulus. Collagen/chitosan scaffolds with different ratio (8:2 and 7:3, V/V), which already showed good properties for cell culture, were tested. The Young's Modulus of collagen/chitosan scaffold with ratio 7:3 is bigger than that of 8:2, which is consistent with our expectation. Force curves were obtained first from indentation, and then Young's Modulus was determined using a proper Hertz contact mathematical model. Meanwhile, the mechanical properties of mice pancreas and heart were obtained as controls. The results indicated that AFM-based nano-indentation is a good method for the mechanical property testing of porous scaffold.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"137 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121414523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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