Pub Date : 2025-01-01DOI: 10.1016/j.wjam.2024.12.009
Jie LI (李杰) , Qiang WANG (王强) , Yuan WANG (王渊) , Jun-yang LIU (刘隽阳) , Jie GUO (郭婕) , Hua LI (李华) , Hao-bin ZHANG (张豪斌) , Jie LIU (刘捷) , An-ren ZHANG (张安仁) , Hai-fa QIAO (乔海法)
Objective
This study aimed to observe the effects of xiusanzhen acupuncture (olfactory three-needling therapy) on the behavior, hippocampal histopathology and microglial (MG) activation of rats with vascular dementia (VD), and to assess the role of the trigeminal nerve.
Methods
Sprague-Dawley (SD) rats were randomly assigned to the sham-operation, model, xiusanzhen, and trigeminal neurotomy groups (n = 15 per group). Bilateral common carotid artery ligation was performed to prepare the VD models. Models of trigeminal neurotomy were prepared through the excision of the frontal nerve and infraorbital nerve. Xiusanzhen acupuncture was delivered via electric stimulation. The acupoints selected were bilateral “Yingxiang (LI20)” and “Yintang (EX-HN3)”, and the stimulation parameters were as follows: disperse-dense wave, at a frequency of 2/15HZ and intensity of 1 mA. The course of treatment was once daily, with one course lasting five days, followed by an interval of two days, yielding a total of four courses. Behavioral changes were detected using the Morris water maze, changes in histomorphology in the hippocampal CA1 region were determined with hematoxylin and eosin (HE) staining, and MG activation in the hippocampal CA1 region was detected using immunofluorescence.
Results
The escape latency: From days 3 to 5, the escape latency was higher in the model group compared to the sham-operation group (P < 0.05), but was lower in the xiusanzhen group compared to the model and trigeminal neurotomy groups (P < 0.05). The frequency for platform crossing and swimming distance: both were reduced in the model group compared to the sham-operation group (P < 0.01); and were elevated in the xiusanzhen group compared to the model group and trigeminal neurotomy group (P < 0.01). Hippocampal pathomorphological changes: In the sham-operation group, the morphological structure and nucleoli were well-defined; in the model group and the trigeminal neurotomy group, cell numbers were reduced and karyopyknosis increased; while in the xiusanzhen group, the cell numbers were elevated and karyopyknosis was reduced compared with the model group. MG: the positive rate was higher in the model group compared to the sham-operation group (P < 0.05); lower in the xiusanzhen group compared to the model group (P < 0.05); and higher in the trigeminal neurotomy group compared to the xiusanzhen group (P < 0.05).
Conclusion
Xiusanzhen acupuncture attenuates cognitive impairment in VD rats, inhibits MG activation, and reduces hippocampal tissue damage. Its effects depend on the structural integrity of the trigeminal nerve.
{"title":"Effects of xiusanzhen acupuncture on hippocampal CA1 region damage and microglia activation in rats with vascular dementia: A trigeminal nerve perspective","authors":"Jie LI (李杰) , Qiang WANG (王强) , Yuan WANG (王渊) , Jun-yang LIU (刘隽阳) , Jie GUO (郭婕) , Hua LI (李华) , Hao-bin ZHANG (张豪斌) , Jie LIU (刘捷) , An-ren ZHANG (张安仁) , Hai-fa QIAO (乔海法)","doi":"10.1016/j.wjam.2024.12.009","DOIUrl":"10.1016/j.wjam.2024.12.009","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to observe the effects of <em>xiusanzhen</em> acupuncture (olfactory three-needling therapy) on the behavior, hippocampal histopathology and microglial (MG) activation of rats with vascular dementia (VD), and to assess the role of the trigeminal nerve.</div></div><div><h3>Methods</h3><div>Sprague-Dawley (SD) rats were randomly assigned to the sham-operation, model, <em>xiusanzhen</em>, and trigeminal neurotomy groups (<em>n</em> = 15 per group). Bilateral common carotid artery ligation was performed to prepare the VD models. Models of trigeminal neurotomy were prepared through the excision of the frontal nerve and infraorbital nerve. <em>Xiusanzhen</em> acupuncture was delivered via electric stimulation. The acupoints selected were bilateral “Yingxiang (LI20)” and “Yintang (EX-HN3)”, and the stimulation parameters were as follows: disperse-dense wave, at a frequency of 2/15HZ and intensity of 1 mA. The course of treatment was once daily, with one course lasting five days, followed by an interval of two days, yielding a total of four courses. Behavioral changes were detected using the Morris water maze, changes in histomorphology in the hippocampal CA1 region were determined with hematoxylin and eosin (HE) staining, and MG activation in the hippocampal CA1 region was detected using immunofluorescence.</div></div><div><h3>Results</h3><div>The escape latency: From days 3 to 5, the escape latency was higher in the model group compared to the sham-operation group (<em>P</em> < 0.05), but was lower in the <em>xiusanzhen</em> group compared to the model and trigeminal neurotomy groups (<em>P</em> < 0.05). The frequency for platform crossing and swimming distance: both were reduced in the model group compared to the sham-operation group (<em>P</em> < 0.01); and were elevated in the <em>xiusanzhen</em> group compared to the model group and trigeminal neurotomy group (<em>P</em> < 0.01). Hippocampal pathomorphological changes: In the sham-operation group, the morphological structure and nucleoli were well-defined; in the model group and the trigeminal neurotomy group, cell numbers were reduced and karyopyknosis increased; while in the <em>xiusanzhen</em> group, the cell numbers were elevated and karyopyknosis was reduced compared with the model group. MG: the positive rate was higher in the model group compared to the sham-operation group (<em>P</em> < 0.05); lower in the <em>xiusanzhen</em> group compared to the model group (<em>P</em> < 0.05); and higher in the trigeminal neurotomy group compared to the <em>xiusanzhen</em> group (<em>P</em> < 0.05).</div></div><div><h3>Conclusion</h3><div><em>Xiusanzhen</em> acupuncture attenuates cognitive impairment in VD rats, inhibits MG activation, and reduces hippocampal tissue damage. Its effects depend on the structural integrity of the trigeminal nerve.</div></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"35 1","pages":"Pages 75-83"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.wjam.2024.12.005
Chen-si ZHENG (郑晨思) , Hui-sheng YANG (杨会生) , Ming-zhao HAO (郝鸣昭) , Si-yu LIU (刘思雨) , Yi-gong FANG (房繄恭)
<div><h3>Objective</h3><div>This study aimed to explore the clinical effect of acupuncture therapy for menstruation regulation and pregnancy promotion on thin endometrium in the real world.</div></div><div><h3>Design</h3><div>This study is a single-center pragmatic randomized controlled trial blinded to the statisticians. Using the “blockrand” software package, based on the age (≥35, <35), 37 patients were randomized into an intervention group (19 cases) and a control group (18 cases). After reassignment regarding patient preference, 21 patients were included in the intervention group and 16 in the control group.</div></div><div><h3>Setting</h3><div>The trial was executed in the Specialty Outpatient Clinic, Acupuncture-Moxibustion Hospital of China Academy of Chinese Medical Sciences, from March 1, 2019, to September 30, 2020.</div></div><div><h3>Participants</h3><div>The study included 37 patients with thin endometrium and without previous acupuncture treatment.</div></div><div><h3>Intervention</h3><div>The intervention group was administered acupuncture for menstruation regulation and pregnancy promotion and a small-dose of progynova (2 mg daily), while the control group was administered a large-dose of progynova (4 mg daily). Interventions started from Day 5 of menstruation until the end of ovulation under B-ultrasound monitoring. The intervention lasted for three menstrual cycles.</div></div><div><h3>Measurements</h3><div>Primary outcomes were changes in endometrial thickness between baseline and after intervention completion and the difference between the two groups after intervention. The secondary outcomes were endometrial and subendometrial blood flow, serum estradiol levels, menstrual conditions, and adverse reactions.</div></div><div><h3>Results</h3><div>(1) Comparison of each indicator before and after intervention completion in the two groups: in the intervention group, the differences were significant in endometrial thickness, menstrual score, estradiol (E2) level in ovulatory period, the pulsatility index (PI) and resistance index (RI) of uterine artery, the ratio of peak systolic velocity to end-diastolic velocity (S/D), the endometrial vascular index (VI), flow index (FI), and vascular flow index (VFI) and volume (<em>P</em> < 0.01). In the control group, significant changes were observed in endometrial thickness, menstrual score, and E2 before and after the intervention (<em>P</em> < 0.05), and no differences were observed in uterine artery PI, RI, S/D and endometrial VI, FI, VFI, and volume (<em>P</em> > 0.05). Compared with the control group, the intervention group showed significant differences in endometrial thickness, menstrual score, E2, uterine artery PI, RI, S/D, and endometrial VI, FI, VFI, and volume after intervention (<em>P</em> < 0.01). No adverse reactions were reported in the intervention group. In contrast, the control group had two cases of nausea and gastrointestinal discomfort after medication, e
{"title":"Effects on the receptivity of thin endometrium treated with acupuncture therapy for menstruation regulation and pregnancy promotion: A pragmatic randomized controlled exploratory study","authors":"Chen-si ZHENG (郑晨思) , Hui-sheng YANG (杨会生) , Ming-zhao HAO (郝鸣昭) , Si-yu LIU (刘思雨) , Yi-gong FANG (房繄恭)","doi":"10.1016/j.wjam.2024.12.005","DOIUrl":"10.1016/j.wjam.2024.12.005","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to explore the clinical effect of acupuncture therapy for menstruation regulation and pregnancy promotion on thin endometrium in the real world.</div></div><div><h3>Design</h3><div>This study is a single-center pragmatic randomized controlled trial blinded to the statisticians. Using the “blockrand” software package, based on the age (≥35, <35), 37 patients were randomized into an intervention group (19 cases) and a control group (18 cases). After reassignment regarding patient preference, 21 patients were included in the intervention group and 16 in the control group.</div></div><div><h3>Setting</h3><div>The trial was executed in the Specialty Outpatient Clinic, Acupuncture-Moxibustion Hospital of China Academy of Chinese Medical Sciences, from March 1, 2019, to September 30, 2020.</div></div><div><h3>Participants</h3><div>The study included 37 patients with thin endometrium and without previous acupuncture treatment.</div></div><div><h3>Intervention</h3><div>The intervention group was administered acupuncture for menstruation regulation and pregnancy promotion and a small-dose of progynova (2 mg daily), while the control group was administered a large-dose of progynova (4 mg daily). Interventions started from Day 5 of menstruation until the end of ovulation under B-ultrasound monitoring. The intervention lasted for three menstrual cycles.</div></div><div><h3>Measurements</h3><div>Primary outcomes were changes in endometrial thickness between baseline and after intervention completion and the difference between the two groups after intervention. The secondary outcomes were endometrial and subendometrial blood flow, serum estradiol levels, menstrual conditions, and adverse reactions.</div></div><div><h3>Results</h3><div>(1) Comparison of each indicator before and after intervention completion in the two groups: in the intervention group, the differences were significant in endometrial thickness, menstrual score, estradiol (E2) level in ovulatory period, the pulsatility index (PI) and resistance index (RI) of uterine artery, the ratio of peak systolic velocity to end-diastolic velocity (S/D), the endometrial vascular index (VI), flow index (FI), and vascular flow index (VFI) and volume (<em>P</em> < 0.01). In the control group, significant changes were observed in endometrial thickness, menstrual score, and E2 before and after the intervention (<em>P</em> < 0.05), and no differences were observed in uterine artery PI, RI, S/D and endometrial VI, FI, VFI, and volume (<em>P</em> > 0.05). Compared with the control group, the intervention group showed significant differences in endometrial thickness, menstrual score, E2, uterine artery PI, RI, S/D, and endometrial VI, FI, VFI, and volume after intervention (<em>P</em> < 0.01). No adverse reactions were reported in the intervention group. In contrast, the control group had two cases of nausea and gastrointestinal discomfort after medication, e","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"35 1","pages":"Pages 50-57"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.wjam.2024.03.001
Shuang-li CHEN (陈双莉) , Li-hong HUANG (黄礼红) , Yu-hong BIAN (边育红) , Yu-ming WANG (王玉明) , Jing-yu ZHANG (张靖宇) , Jin-yu LIAN (廉金玉) , Ya-man ZHENG (郑雅蔓) , Zi-yang FAN (樊紫阳) , Xin-ru YUAN (袁馨如) , Xiao-yan LYU (吕笑颜) , Pei-rong LUO (罗佩蓉) , Yu-xin FANG (房钰鑫) , Li-yuan FU (傅丽媛) , Ji-wen QIU (邱继文) , Xiao-wei LIN (林小伟) , Ze-lin CHEN (陈泽林) , Lian-qi GENG (耿连歧) , Yi GUO (郭义) , Ning-cen LI (李柠岑) , Bo CHEN (陈波)
Sepsis is characterized by immune dysregulation that are responsible for an increase in secondary infections and mortality. Acupuncture is a potential alternative treatment for sepsis. In this comprehensive literature review, we found that acupuncture is beneficial in treating immune disorders associated with sepsis. Acupuncture can improve immune disorders associated with sepsis and regulate the functions of innate and adaptive immune cells. Specifically, acupuncture can reduce the number of neutrophils in sepsis, promote the polarization of macrophages towards M2-like macrophages, and alleviate inflammation by reducing the activation of microglia and astrocytes. Furthermore, acupuncture can increase the percentage of T cells and modulate the balance between T cell subsets. The immunomodulatory mechanism of acupuncture in sepsis may be attributed to the balance of the autonomic nervous system, including activation of the sympathetic-adrenal axis, vagal-cholinergic pathway, and vagal-adrenal axis. In addition, acupuncture can inhibit inflammation by preserving the integrity of the intestinal barrier and regulating the composition of the intestinal microbiota. Clinical studies have also demonstrated that acupuncture can enhance the number of peripheral natural killer (NK) cells and T cell subsets, as well as the expression of human leukocyte antigen DR (HLA-DR). Moreover, acupuncture can decrease the ratio of white blood cells to neutrophils and reduce the levels of inflammatory factors. Therefore, acupuncture has the potential to improve immune function in sepsis. Further investigation of its mechanism is expected to provide a scientific and reliable foundation for the application of acupuncture in sepsis treatment.
败血症的特点是免疫功能失调，是继发性感染和死亡率增加的原因。针灸是治疗败血症的一种潜在替代疗法。在这次全面的文献综述中，我们发现针灸对治疗败血症相关的免疫功能紊乱有益。针灸可以改善败血症相关的免疫紊乱，调节先天性免疫细胞和适应性免疫细胞的功能。具体来说，针灸可以减少败血症患者中性粒细胞的数量，促进巨噬细胞向 M2 样巨噬细胞极化，并通过减少小胶质细胞和星形胶质细胞的活化来缓解炎症。此外，针灸还能提高 T 细胞的比例，调节 T 细胞亚群之间的平衡。针灸对败血症的免疫调节机制可能归因于自律神经系统的平衡，包括交感-肾上腺轴、迷走-胆碱能通路和迷走-肾上腺轴的激活。此外，针灸还能通过保护肠道屏障的完整性和调节肠道微生物群的组成来抑制炎症。临床研究还表明，针灸可以增强外周自然杀伤细胞（NK）和 T 细胞亚群的数量，以及人类白细胞抗原 DR（HLA-DR）的表达。此外，针灸还能降低白细胞与中性粒细胞的比例，降低炎症因子的水平。因此，针灸具有改善败血症患者免疫功能的潜力。对其机理的进一步研究有望为针灸在败血症治疗中的应用提供科学可靠的依据。
{"title":"Acupuncture therapy: A potential new strategy for immunosuppressive sepsis","authors":"Shuang-li CHEN (陈双莉) , Li-hong HUANG (黄礼红) , Yu-hong BIAN (边育红) , Yu-ming WANG (王玉明) , Jing-yu ZHANG (张靖宇) , Jin-yu LIAN (廉金玉) , Ya-man ZHENG (郑雅蔓) , Zi-yang FAN (樊紫阳) , Xin-ru YUAN (袁馨如) , Xiao-yan LYU (吕笑颜) , Pei-rong LUO (罗佩蓉) , Yu-xin FANG (房钰鑫) , Li-yuan FU (傅丽媛) , Ji-wen QIU (邱继文) , Xiao-wei LIN (林小伟) , Ze-lin CHEN (陈泽林) , Lian-qi GENG (耿连歧) , Yi GUO (郭义) , Ning-cen LI (李柠岑) , Bo CHEN (陈波)","doi":"10.1016/j.wjam.2024.03.001","DOIUrl":"10.1016/j.wjam.2024.03.001","url":null,"abstract":"<div><div>Sepsis is characterized by immune dysregulation that are responsible for an increase in secondary infections and mortality. Acupuncture is a potential alternative treatment for sepsis. In this comprehensive literature review, we found that acupuncture is beneficial in treating immune disorders associated with sepsis. Acupuncture can improve immune disorders associated with sepsis and regulate the functions of innate and adaptive immune cells. Specifically, acupuncture can reduce the number of neutrophils in sepsis, promote the polarization of macrophages towards M2-like macrophages, and alleviate inflammation by reducing the activation of microglia and astrocytes. Furthermore, acupuncture can increase the percentage of T cells and modulate the balance between T cell subsets. The immunomodulatory mechanism of acupuncture in sepsis may be attributed to the balance of the autonomic nervous system, including activation of the sympathetic-adrenal axis, vagal-cholinergic pathway, and vagal-adrenal axis. In addition, acupuncture can inhibit inflammation by preserving the integrity of the intestinal barrier and regulating the composition of the intestinal microbiota. Clinical studies have also demonstrated that acupuncture can enhance the number of peripheral natural killer (NK) cells and T cell subsets, as well as the expression of human leukocyte antigen DR (HLA-DR). Moreover, acupuncture can decrease the ratio of white blood cells to neutrophils and reduce the levels of inflammatory factors. Therefore, acupuncture has the potential to improve immune function in sepsis. Further investigation of its mechanism is expected to provide a scientific and reliable foundation for the application of acupuncture in sepsis treatment.</div></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"35 1","pages":"Pages 17-26"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.wjam.2025.01.002
Xiao-lei ZHANG (张小蕾), Meng-ni HU (胡梦妮), Ya-nan LI (李亚楠), Yao WANG (汪瑶), Zhen RONG (荣臻), Jun MA (马骏)
<div><h3>Objective</h3><div>To investigate the effects of electroacupuncture (EA) on the blood-brain barrier permeability and the regulation of matrix metalloproteinases (MMPs) in the mice with Parkinson's disease (PD).</div></div><div><h3>Methods</h3><div>Forty-eight C57BL/6 mice were randomly assigned into the normal control (NC) group, the PD model (PD) group, the EA group and the EA + SB-3CT inhibitor group (EA + SB-3CT), with 12 mice in each group. In this experiment, the PD model was established by intragastric administration (IG) with rotenone for 4 wk in the PD group, EA group and EA+SB-3CT group. In the EA + SB-3CT group, 1 h after IG with rotenone, the mice were intraperitoneally injected with MMP-2/9 inhibitor, SB-3CT (25 mg/kg/d). After successfully modeled, in the EA group and EA + SB-3CT group, EA was conducted at “Fengfu (GV16)” and bilateral “Taichong (LR3)” and “Zusanli (ST36)”, at 1 mA and 2 Hz for 30 min each time, once a day, for consecutive 2 wk. The behavioral changes of the mice were observed in each group using the open field test, the level of tyrosine hydroxylase (TH) in the substantia nigra was determined by immunohistochemistry, the permeability of the blood-brain barrier was detected by Evans blue staining, and the protein expression of ZO-1, ocludin, claudin-1, MMP-2 and MMP-9 in the substantia nigra was detected by Western blotting.</div></div><div><h3>Results</h3><div>Compared with the NC group, the behavioral scores increased (<em>P</em> < 0.05), while total time of locomotion, total distance and average speed were reduced (<em>P</em> < 0.05) in the PD group. The expression of TH in the substantia nigra decreased (<em>P</em> < 0.05), Evans blue level in the brain tissue increased (<em>P</em> < 0.05), and the protein expression of ZO-1, occludin and claudin-1 was lower (<em>P</em> < 0.05), whereas MMP-2 and MMP-9 expression was higher (<em>P</em> < 0.05) in the PD group. Compared with the PD group, behavioral scores decreased (<em>P</em> < 0.05), while the total time of locomotion, total distance and average speed increased (<em>P</em> < 0.05) in the EA group. Additionally, TH expression in the substantia nigra was elevated (<em>P</em> < 0.05), Evans blue level in the brain tissue was lower (<em>P</em> < 0.05), the protein expression of ZO-1, occludin and claudin-1 was up-regulated (<em>P</em> < 0.05), and MMP-2 and MMP-9 expression was down-regulated (<em>P</em> < 0.05) in the EA group. Compared with the EA group, Evans blue level was reduced (<em>P</em> < 0.05), the protein expression of ZO-1 and occludin was up-regulated (<em>P</em> < 0.05), and MMP-2 and MMP-9 expression was further down-regulated (<em>P</em> < 0.05) in the EA+SB-3CT group.</div></div><div><h3>Conclusion</h3><div>EA can effectively ameliorate the motor dysfunction of PD mice, reduce the damage of dopaminergic neurons, and play a neuroprotective role. EA can effectively improve the blood–brain barrier
{"title":"Effect of electroacupuncture on the MMP-2/9 pathway mediated blood-brain barrier permeability in mice with Parkinson's disease","authors":"Xiao-lei ZHANG (张小蕾), Meng-ni HU (胡梦妮), Ya-nan LI (李亚楠), Yao WANG (汪瑶), Zhen RONG (荣臻), Jun MA (马骏)","doi":"10.1016/j.wjam.2025.01.002","DOIUrl":"10.1016/j.wjam.2025.01.002","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effects of electroacupuncture (EA) on the blood-brain barrier permeability and the regulation of matrix metalloproteinases (MMPs) in the mice with Parkinson's disease (PD).</div></div><div><h3>Methods</h3><div>Forty-eight C57BL/6 mice were randomly assigned into the normal control (NC) group, the PD model (PD) group, the EA group and the EA + SB-3CT inhibitor group (EA + SB-3CT), with 12 mice in each group. In this experiment, the PD model was established by intragastric administration (IG) with rotenone for 4 wk in the PD group, EA group and EA+SB-3CT group. In the EA + SB-3CT group, 1 h after IG with rotenone, the mice were intraperitoneally injected with MMP-2/9 inhibitor, SB-3CT (25 mg/kg/d). After successfully modeled, in the EA group and EA + SB-3CT group, EA was conducted at “Fengfu (GV16)” and bilateral “Taichong (LR3)” and “Zusanli (ST36)”, at 1 mA and 2 Hz for 30 min each time, once a day, for consecutive 2 wk. The behavioral changes of the mice were observed in each group using the open field test, the level of tyrosine hydroxylase (TH) in the substantia nigra was determined by immunohistochemistry, the permeability of the blood-brain barrier was detected by Evans blue staining, and the protein expression of ZO-1, ocludin, claudin-1, MMP-2 and MMP-9 in the substantia nigra was detected by Western blotting.</div></div><div><h3>Results</h3><div>Compared with the NC group, the behavioral scores increased (<em>P</em> < 0.05), while total time of locomotion, total distance and average speed were reduced (<em>P</em> < 0.05) in the PD group. The expression of TH in the substantia nigra decreased (<em>P</em> < 0.05), Evans blue level in the brain tissue increased (<em>P</em> < 0.05), and the protein expression of ZO-1, occludin and claudin-1 was lower (<em>P</em> < 0.05), whereas MMP-2 and MMP-9 expression was higher (<em>P</em> < 0.05) in the PD group. Compared with the PD group, behavioral scores decreased (<em>P</em> < 0.05), while the total time of locomotion, total distance and average speed increased (<em>P</em> < 0.05) in the EA group. Additionally, TH expression in the substantia nigra was elevated (<em>P</em> < 0.05), Evans blue level in the brain tissue was lower (<em>P</em> < 0.05), the protein expression of ZO-1, occludin and claudin-1 was up-regulated (<em>P</em> < 0.05), and MMP-2 and MMP-9 expression was down-regulated (<em>P</em> < 0.05) in the EA group. Compared with the EA group, Evans blue level was reduced (<em>P</em> < 0.05), the protein expression of ZO-1 and occludin was up-regulated (<em>P</em> < 0.05), and MMP-2 and MMP-9 expression was further down-regulated (<em>P</em> < 0.05) in the EA+SB-3CT group.</div></div><div><h3>Conclusion</h3><div>EA can effectively ameliorate the motor dysfunction of PD mice, reduce the damage of dopaminergic neurons, and play a neuroprotective role. EA can effectively improve the blood–brain barrier","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"35 1","pages":"Pages 58-65"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.wjam.2024.12.008
Xiang-yu HU (胡翔昱), Wen-cui XIU (修文萃), Zi-yu TIAN (田紫煜), Rui-min JIAO (焦睿珉), Huan CHEN (陈欢), Xiao-yi HU (胡晓艺), Lan-jun SHI (施兰君), Tian-yu MING (明恬妤), Wei-juan GANG (岗卫娟), Xiang-hong JING (景向红)
Background
Methodological issues and challenges hinder the high-quality development of acupuncture clinical studies.
Objective
To systematically summarize the methodological issues, challenges, and recommendations in acupuncture clinical research using the participant, intervention, comparison, outcome, and study design (PICOS) framework to generate a comprehensive list of methodological questions and recommendations.
Methods
We searched seven databases for articles on the methodological aspects of clinical acupuncture research from their inception to March 24, 2023. Two researchers independently screened the titles, abstracts, and full text of each article. Data extraction and analyses were performed for the eligible studies. Finally, the methodological challenges and recommendations for acupuncture clinical studies are listed, and the frequency of methodological challenges is displayed in a sunburst chart.
Results
A total of 226 studies were included in the review, most of which originated in China (173, 77 %), followed by the United States (25, 11 %) and the United Kingdom (12, 5 %). Clinical research on acupuncture faces methodological challenges that span the five elements of the PICOS framework. The most frequently mentioned issues were related to comparison, followed by study design and interventions. If further categorized, the establishment of the control group is undoubtedly the most concerning issue for researchers.
Conclusion
This review provides a comprehensive overview of the challenges and recommendations for each aspect of the PICOS framework in clinical acupuncture research.
{"title":"Methodological challenges and recommendations for acupuncture clinical study: A scoping review","authors":"Xiang-yu HU (胡翔昱), Wen-cui XIU (修文萃), Zi-yu TIAN (田紫煜), Rui-min JIAO (焦睿珉), Huan CHEN (陈欢), Xiao-yi HU (胡晓艺), Lan-jun SHI (施兰君), Tian-yu MING (明恬妤), Wei-juan GANG (岗卫娟), Xiang-hong JING (景向红)","doi":"10.1016/j.wjam.2024.12.008","DOIUrl":"10.1016/j.wjam.2024.12.008","url":null,"abstract":"<div><h3>Background</h3><div>Methodological issues and challenges hinder the high-quality development of acupuncture clinical studies.</div></div><div><h3>Objective</h3><div>To systematically summarize the methodological issues, challenges, and recommendations in acupuncture clinical research using the participant, intervention, comparison, outcome, and study design (PICOS) framework to generate a comprehensive list of methodological questions and recommendations.</div></div><div><h3>Methods</h3><div>We searched seven databases for articles on the methodological aspects of clinical acupuncture research from their inception to March 24, 2023. Two researchers independently screened the titles, abstracts, and full text of each article. Data extraction and analyses were performed for the eligible studies. Finally, the methodological challenges and recommendations for acupuncture clinical studies are listed, and the frequency of methodological challenges is displayed in a sunburst chart.</div></div><div><h3>Results</h3><div>A total of 226 studies were included in the review, most of which originated in China (173, 77 %), followed by the United States (25, 11 %) and the United Kingdom (12, 5 %). Clinical research on acupuncture faces methodological challenges that span the five elements of the PICOS framework. The most frequently mentioned issues were related to comparison, followed by study design and interventions. If further categorized, the establishment of the control group is undoubtedly the most concerning issue for researchers.</div></div><div><h3>Conclusion</h3><div>This review provides a comprehensive overview of the challenges and recommendations for each aspect of the PICOS framework in clinical acupuncture research.</div></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"35 1","pages":"Pages 1-9"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.wjam.2024.12.004
Tian-yu GAO (高田宇) , Fei-cheng ZHANG (张飞程) , Ru-fang PAN (潘茹芳) , Shu-ya MA (马舒雅) , Xiao-qi ZHANG (张晓琪) , Li-jia PAN (潘丽佳) , Chun-sheng JIA (贾春生)
Objective
The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were evaluated. Changes in the co-stimulatory molecules CD80 and CD86 on antigen-presenting cells in gastric tissues as well as related cytokines in serum were evaluated. The aim of the study was to explore the immunological mechanisms by which direct moxibustion may prevent gastric cancer lesions, thereby providing a basis for studies on the immunological mechanisms by which moxibustion prevents tumor development.
Methods
Sixty healthy male Wistar rats were randomly divided into four groups: Control, control+moxibustion, model, and moxibustion groups. A gastric cancer rat model was induced by intragastric administration of 20 mg/mL MNNG, with a dose of 1 mL/100 g body weight, once daily for 16 weeks. The control+moxibustion and moxibustion groups received direct moxibustion simultaneously with modeling, continuing for 16 weeks. After the experiment, gastric tissue was collected, and morphological changes in the gastric mucosa in each group of rats were observed through H&E staining. Immunohistochemistry (IHC) and a western blotting were used to detect the expression levels of CD80 and CD86 in gastric tissues. Enzyme-linked immunosorbent assays (ELISA) were used to measure the levels of interleukin-12 (IL-12), interferon-gamma (IFN-γ), and tumor necrosis factor-beta (TNF-β) in rat serum.
Results
Upon macroscopic observation, the gastric mucosa of rats in the control and control+moxibustion groups appeared uniformly red, with a glossy mucosal surface, normal gastric wall elasticity, and clear, regular mucosal folds, without hyperplasia or bleeding points. In the model group, the gastric mucosa was reduced in volume, the gastric wall thinned, elasticity decreased, mucosal folds were disordered, and yellow-white cauliflower-like lesions and yellow-brown hyperkeratosis were observed. In the moxibustion group, the gastric mucosa showed decreased elasticity, with disordered mucosal folds and granular hyperplasia. After H&E staining, the gastric mucosal structure was clear and intact in the control and control+moxibustion groups displaying an organized and uniform arrangement of the mucosa, submucosa, and muscularis propria, without hyperplasia or keratinization. In the model group, the epithelial glands in the gastric mucosa were disordered, with varied cell morphologies, thickened submucosa, and disrupted squamous epithelium that invaded downward into the muscularis propria. In the moxibustion group, the squamous epithelium did not invade the muscularis propria. IHC results showed higher expression levels of CD80 and CD86 in the gastric mucosa of the control+moxibustion group than in the control group (P < 0.05) and lower expression levels in
{"title":"Effects of direct moxibustion on antigen-presenting cells in gastric tissue of rat models with gastric cancer: Understanding the immunological mechanisms","authors":"Tian-yu GAO (高田宇) , Fei-cheng ZHANG (张飞程) , Ru-fang PAN (潘茹芳) , Shu-ya MA (马舒雅) , Xiao-qi ZHANG (张晓琪) , Li-jia PAN (潘丽佳) , Chun-sheng JIA (贾春生)","doi":"10.1016/j.wjam.2024.12.004","DOIUrl":"10.1016/j.wjam.2024.12.004","url":null,"abstract":"<div><h3>Objective</h3><div>The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of <em>N</em>-methyl-<em>N</em>'-nitro-<em>N</em>-nitrosoguanidine (MNNG) were evaluated. Changes in the co-stimulatory molecules CD80 and CD86 on antigen-presenting cells in gastric tissues as well as related cytokines in serum were evaluated. The aim of the study was to explore the immunological mechanisms by which direct moxibustion may prevent gastric cancer lesions, thereby providing a basis for studies on the immunological mechanisms by which moxibustion prevents tumor development.</div></div><div><h3>Methods</h3><div>Sixty healthy male Wistar rats were randomly divided into four groups: Control, control+moxibustion, model, and moxibustion groups. A gastric cancer rat model was induced by intragastric administration of 20 mg/mL MNNG, with a dose of 1 mL/100 g body weight, once daily for 16 weeks. The control+moxibustion and moxibustion groups received direct moxibustion simultaneously with modeling, continuing for 16 weeks. After the experiment, gastric tissue was collected, and morphological changes in the gastric mucosa in each group of rats were observed through H&E staining. Immunohistochemistry (IHC) and a western blotting were used to detect the expression levels of CD80 and CD86 in gastric tissues. Enzyme-linked immunosorbent assays (ELISA) were used to measure the levels of interleukin-12 (IL-12), interferon-gamma (IFN-γ), and tumor necrosis factor-beta (TNF-β) in rat serum.</div></div><div><h3>Results</h3><div>Upon macroscopic observation, the gastric mucosa of rats in the control and control+moxibustion groups appeared uniformly red, with a glossy mucosal surface, normal gastric wall elasticity, and clear, regular mucosal folds, without hyperplasia or bleeding points. In the model group, the gastric mucosa was reduced in volume, the gastric wall thinned, elasticity decreased, mucosal folds were disordered, and yellow-white cauliflower-like lesions and yellow-brown hyperkeratosis were observed. In the moxibustion group, the gastric mucosa showed decreased elasticity, with disordered mucosal folds and granular hyperplasia. After H&E staining, the gastric mucosal structure was clear and intact in the control and control+moxibustion groups displaying an organized and uniform arrangement of the mucosa, submucosa, and muscularis propria, without hyperplasia or keratinization. In the model group, the epithelial glands in the gastric mucosa were disordered, with varied cell morphologies, thickened submucosa, and disrupted squamous epithelium that invaded downward into the muscularis propria. In the moxibustion group, the squamous epithelium did not invade the muscularis propria. IHC results showed higher expression levels of CD80 and CD86 in the gastric mucosa of the control+moxibustion group than in the control group (<em>P</em> < 0.05) and lower expression levels in ","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"35 1","pages":"Pages 66-74"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To construct a clinical prediction model of acupuncture treatment for diminished ovarian reserve (DOR) based on a machine learning algorithm to provide a clinical prediction of acupuncture for ameliorating pregnancy outcomes in DOR.
Methods
We enrolled 377 DOR patients treated with acupuncture and with records of pregnancy outcomes (139 cases of pregnancy and 238 cases failed) exported from the International Patient Registry Platform of Acupuncture-moxibustion (IPRPAM). The predictive variables were determined using Spearman's correlation analysis and feature engineering methods. The model was constructed by adopting logistic regression, naïve Bayes, random forest, support vector machine, extreme gradient boosting, the k-nearest neighbor algorithm, linear discriminant analysis, and neural network methods. The models were validated by the area under the curve (AUC), accuracy (ACC), and importance sequencing, and individual pregnancy prediction was conducted for the best-performing model.
Results
The key factors determining pregnancy after acupuncture in patients with DOR were age, luteinizing hormone (LH) level after treatment, follicle-stimulating hormone (FSH) level after treatment, the ratio of FSH to LH (FSH/LH) after treatment, and history of acupuncture treatment. Random forest model ACC was 0.95, Fβ was 0.93, Logloss was 0.30, Logloss value was the lowest, the model variables exhibited the highest accuracy and precision.
Conclusion
The random forest model for the effects of acupuncture on pregnancy outcomes in patients with DOR, constructed based on the IPRPAM, presents a favorable value for clinical application.
Trial registration: Registration number in the Chinese Clinical Trial Registry Center
{"title":"Construction of a pregnancy prediction model in acupuncture treatment for diminished ovarian reserve based on machine learning","authors":"Ming-hui GOU (勾明会) , Hui-sheng YANG (杨会生) , Yi-gong FANG (房繄恭)","doi":"10.1016/j.wjam.2025.01.001","DOIUrl":"10.1016/j.wjam.2025.01.001","url":null,"abstract":"<div><h3>Objective</h3><div>To construct a clinical prediction model of acupuncture treatment for diminished ovarian reserve (DOR) based on a machine learning algorithm to provide a clinical prediction of acupuncture for ameliorating pregnancy outcomes in DOR.</div></div><div><h3>Methods</h3><div>We enrolled 377 DOR patients treated with acupuncture and with records of pregnancy outcomes (139 cases of pregnancy and 238 cases failed) exported from the International Patient Registry Platform of Acupuncture-moxibustion (IPRPAM). The predictive variables were determined using Spearman's correlation analysis and feature engineering methods. The model was constructed by adopting logistic regression, naïve Bayes, random forest, support vector machine, extreme gradient boosting, the k-nearest neighbor algorithm, linear discriminant analysis, and neural network methods. The models were validated by the area under the curve (AUC), accuracy (ACC), and importance sequencing, and individual pregnancy prediction was conducted for the best-performing model.</div></div><div><h3>Results</h3><div>The key factors determining pregnancy after acupuncture in patients with DOR were age, luteinizing hormone (LH) level after treatment, follicle-stimulating hormone (FSH) level after treatment, the ratio of FSH to LH (FSH/LH) after treatment, and history of acupuncture treatment. Random forest model ACC was 0.95, Fβ was 0.93, Logloss was 0.30, Logloss value was the lowest, the model variables exhibited the highest accuracy and precision.</div></div><div><h3>Conclusion</h3><div>The random forest model for the effects of acupuncture on pregnancy outcomes in patients with DOR, constructed based on the IPRPAM, presents a favorable value for clinical application.</div></div><div><h3>Trial registration: Registration number in the Chinese Clinical Trial Registry Center</h3><div>ChiCTR2200062293.</div></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"35 1","pages":"Pages 32-40"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.wjam.2024.12.001
Xiao-yu LIU (刘晓玉) , Pei-hong MA (马培宏) , Bao-yan LIU (刘保延) , Shi-yan YAN (闫世艳)
Sham acupuncture, as a control intervention in acupuncture studies, has been widely conducted to evaluate the specific effect of acupuncture. However, there was no relevant particular reporting guideline, so the reporting quality of sham acupuncture was low, which could hinder the precise understanding and interpretation of details and implementation background of sham acupuncture by researchers. In order to standardize the reporting of sham acupuncture and improve its quality, SHam Acupuncture REporting guidelines and a checklist in clinical trials (SHARE) was developed by a research team from Beijing University of Chinese Medicine. The SAHRE standardizes the reporting of sham acupuncture from 10 categories, including brief name, rationales, details of sham acupuncture, treatment regimen of sham acupuncture, the information informed or explained to patients, practitioner information, modifications of protocol, communication between practitioner and patient, practitioner adherence and blinding. This article elaborates the development process and key content of SHARE in order to facilitate its application by researchers.
{"title":"Interpretation of SHARE: SHam Acupuncture REporting guidelines and a checklist in clinical trials","authors":"Xiao-yu LIU (刘晓玉) , Pei-hong MA (马培宏) , Bao-yan LIU (刘保延) , Shi-yan YAN (闫世艳)","doi":"10.1016/j.wjam.2024.12.001","DOIUrl":"10.1016/j.wjam.2024.12.001","url":null,"abstract":"<div><div>Sham acupuncture, as a control intervention in acupuncture studies, has been widely conducted to evaluate the specific effect of acupuncture. However, there was no relevant particular reporting guideline, so the reporting quality of sham acupuncture was low, which could hinder the precise understanding and interpretation of details and implementation background of sham acupuncture by researchers. In order to standardize the reporting of sham acupuncture and improve its quality, <em>SHam Acupuncture REporting guidelines and a checklist in clinical trials (SHARE)</em> was developed by a research team from Beijing University of Chinese Medicine. The SAHRE standardizes the reporting of sham acupuncture from 10 categories, including brief name, rationales, details of sham acupuncture, treatment regimen of sham acupuncture, the information informed or explained to patients, practitioner information, modifications of protocol, communication between practitioner and patient, practitioner adherence and blinding. This article elaborates the development process and key content of SHARE in order to facilitate its application by researchers.</div></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"35 1","pages":"Pages 27-31"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1016/j.wjam.2024.09.002
Dai-ming FAN (樊代明)
Holistic integrative medicine, abbreviated as HIM, has been officially proposed since 2012. Its theoretical system has been continuously improved, and its practical methods have become increasingly diverse, becoming an inevitable choice and path for the medical development in the new era. This article demonstrates ten major propositions for HIM, elaborating on the connotation and extension of HIM from the perspectives of epistemology and methodology, in order to achieve the transformation and adaptive evolution of modern medicine.
{"title":"Holistic integrative medicine declaration","authors":"Dai-ming FAN (樊代明)","doi":"10.1016/j.wjam.2024.09.002","DOIUrl":"10.1016/j.wjam.2024.09.002","url":null,"abstract":"<div><div>Holistic integrative medicine, abbreviated as HIM, has been officially proposed since 2012. Its theoretical system has been continuously improved, and its practical methods have become increasingly diverse, becoming an inevitable choice and path for the medical development in the new era. This article demonstrates ten major propositions for HIM, elaborating on the connotation and extension of HIM from the perspectives of epistemology and methodology, in order to achieve the transformation and adaptive evolution of modern medicine.</div></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"34 4","pages":"Pages 332-333"},"PeriodicalIF":0.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To systematically evaluate the effectiveness of acupuncture and moxibustion as a complementary treatment for polycystic ovary syndrome(PCOS) and provide evidence for clinical treatment.
Methods
The following seven databases were searched from the date of database creation to October 29, 2023: PubMed, Embase, the Cochrane Library, China Biology Medicine (CBM), Chinese National Knowledge Infrastructure (CNKI), Wanfang, and VIP. The literature was screened according to the inclusion and exclusion criteria, data were extracted, quality was evaluated using the Cochrane Risk of Bias Assessment Tool, and a meta-analysis of the outcomes was performed using Review Manager 5.4 software.
Results
A total of 20 researches were included and the test group was treated with modern medicine, acupuncture, electroacupuncture, warm needling, and thunder-fire moxibustion. The control group was treated with modern medicine, including clomiphene, Diane-35, letrozole, metformin, and progesterone. The meta-analysis showed that acupuncture and moxibustion as a complementary therapy for PCOS improved the ovulation and pregnancy rates and reduced the body mass index (BMI), luteinizing hormone (LH) levels, the LH/follicle-stimulating hormone (FSH) ratio, and ovarian volume; however, due to high heterogeneity, the differences regarding improvements in FSH, estradiol(E2), testosterone (T) and progesterone (P) levels could not draw a definite conclusion when compared with modern first-line drugs alone.
Conclusion
Acupuncture and moxibustion as a complementary treatment for PCOS has good clinical efficacy; however, because of the relatively low quality of the included studies, the results may be biased. More high-quality clinical studies are needed to verify the results. The Prospero Registration number is CRD42022368304.
{"title":"Meta-analysis of the efficacy of acupuncture and moxibustion in the treatment of polycystic ovary syndrome","authors":"Dong-mei JIANG(江冬梅) , Hong LIN(林虹) , Zhi-feng YU(于志峰) , Meng WANG(王萌)","doi":"10.1016/j.wjam.2024.09.006","DOIUrl":"10.1016/j.wjam.2024.09.006","url":null,"abstract":"<div><h3>Objective</h3><div>To systematically evaluate the effectiveness of acupuncture and moxibustion as a complementary treatment for polycystic ovary syndrome(PCOS) and provide evidence for clinical treatment.</div></div><div><h3>Methods</h3><div>The following seven databases were searched from the date of database creation to October 29, 2023: PubMed, Embase, the Cochrane Library, China Biology Medicine (CBM), Chinese National Knowledge Infrastructure (CNKI), Wanfang, and VIP. The literature was screened according to the inclusion and exclusion criteria, data were extracted, quality was evaluated using the Cochrane Risk of Bias Assessment Tool, and a meta-analysis of the outcomes was performed using Review Manager 5.4 software.</div></div><div><h3>Results</h3><div>A total of 20 researches were included and the test group was treated with modern medicine, acupuncture, electroacupuncture, warm needling, and thunder-fire moxibustion. The control group was treated with modern medicine, including clomiphene, Diane-35, letrozole, metformin, and progesterone. The meta-analysis showed that acupuncture and moxibustion as a complementary therapy for PCOS improved the ovulation and pregnancy rates and reduced the body mass index (BMI), luteinizing hormone (LH) levels, the LH/follicle-stimulating hormone (FSH) ratio, and ovarian volume; however, due to high heterogeneity, the differences regarding improvements in FSH, estradiol(E2), testosterone (T) and progesterone (P) levels could not draw a definite conclusion when compared with modern first-line drugs alone.</div></div><div><h3>Conclusion</h3><div>Acupuncture and moxibustion as a complementary treatment for PCOS has good clinical efficacy; however, because of the relatively low quality of the included studies, the results may be biased. More high-quality clinical studies are needed to verify the results. The Prospero Registration number is CRD42022368304.</div></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"34 4","pages":"Pages 282-291"},"PeriodicalIF":0.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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