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A Small Redbean Cultivar ‘Hongjin’ with Lodging Tolerance and High Yield 耐倒伏高产小红豆品种‘红锦’
Pub Date : 2019-12-01 DOI: 10.9787/kjbs.2019.51.4.523
Song Seok-Bo, Ko Jee-Yeon, Woo KoanSik, Choe MyeongEn, Chu Jiho, Ha TaeJoung, Han, Sangik, Kwak DoYeon
A new small redbean cultivar, ‘Hongjin’, was developed by an artificial cross between ‘SA9206-2B-6-1-2-3-3-2’ and ‘Suwon38’ at the National Institute of Crop Science, RDA, Korea, in 1999, and selected for the further yield trials under the name ‘Miryang 23’. It was prominent and had desirable agronomic traits, such as high grain quality, lodging tolerance, and high antioxidant activity in the regional adaptation yield trials (RYT) from 2013 to 2015. ‘Hongjin’ was finally released as a new cultivar in 2015. ‘Hongjin’ has some distinct phenotypic traits, including a semi-determinate growth habit, yellow flowers, green embryonic axis, dark red seed coat, white hilum, and small spherical seeds (15.4 g per 100 seeds). The average yield of ‘Hongjin’ was 1.99 MT/ha in the regional yield trials (RYT), which was slightly higher than that of the check cultivar ‘Chungju’ (1.84 MT/ha) (Registration No. 7510).
1999年,韩国农林部作物科学研究所将‘sa9206 - 2b -6-1-2-3- 2’与‘水原38’人工杂交,培育出一种新的红豆品种‘红进’,并将其命名为‘密阳23’进行进一步的产量试验。在2013 ~ 2015年的区域适应产量试验(RYT)中,该品种具有优良的籽粒品质、耐倒伏、抗氧化活性等农艺性状。“红锦”终于在2015年作为新品种发布。‘红金’有一些明显的表型特征,包括半确定的生长习性,黄色的花,绿色的胚轴,暗红色的种皮,白色的种门和小的球形种子(每100粒种子15.4克)。在区域产量试验(RYT)中,‘红津’的平均产量为1.99吨/公顷,略高于对照品种‘忠州’(登记号7510)的1.84吨/公顷。
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引用次数: 4
An Early, Cold-Tolerant and High-Quality Rice ‘Jinhan’ Adaptable to Northern Central Highland 适合中部高原北部地区的耐冷早熟优质水稻金汉
Pub Date : 2019-12-01 DOI: 10.9787/kjbs.2019.51.4.489
Ung-Jo Hyun, Jeong-Heui Lee, E. Jeong, Jae-Ki Chang, Kuk-Hyun Jung, Young-chan Cho, J. Lee, Y. Won, J. Suh, E. Ahn, Jong‐Min Jeong, Y. Jeon, Dae-Ha Seo, and Jeong-Ju Kim
Jinhan’, a japonica rice variety, was developed from a cross between ‘Cheolwon68’ (IT218244) and ‘Junghwabyeo’ (IT260473) by the rice breeding team at National Institute of Crop Science in 2015. ‘Cheolwon68’ is early maturing and resistant to blast disease, and ‘Junghwabyeo’ is a cold-tolerant and high-quality variety. The growth duration of ‘Jinhan’ in the paddy field was 116 days in the Northern Central Highland in Korea, which was similar to that of ‘Jinbu’. The culm length of ‘Jinhan’ was 61 cm, which was 10 cm shorter than that of ‘Jinbu’. The panicle of ‘Jinhan’ was composed of 66 spikelets. The viviparous germination rate of ‘Jinhan’ was 33.2%. ‘Jinhan’ showed resistance to blast disease, but was susceptible to stripe virus, dwarf and black-streaked dwarf viruses, and plant hoppers. The milled rice grains of ‘Jinhan’ showed an excellent palatability index (0.23) and lower protein content (5.7%) than that of ‘Jinbu’. The grain milling characteristics of ‘Jinhan’ were similar to those of ‘Jinbu’. ‘Jinhan’ showed a milled rice productivity of 5.43 MT/ha at four sites cultivated in the Northern Central Highland (Registration No. 7318).
粳稻品种“金韩”是国立作物科学研究院水稻育种组在2015年以“铁原68”(IT218244)和“正花育”(IT260473)杂交而成的品种。“铁原68”是早熟的抗稻瘟病品种,“正花玉”是耐寒的优质品种。在韩国中部北部高地的水田中,‘晋寒’的生长期为116天,与‘晋布’相似。‘金含’的茎长为61 cm,比‘金布’短10 cm。金涵的穗部由66个小穗组成。金汉的胎生发芽率为33.2%。金汉对稻瘟病表现出抗性,但对条纹病毒、矮缩病毒和黑条纹矮缩病毒以及植物跳虫易感。“晋寒”的食味指数为0.23,蛋白质含量为5.7%,低于“晋布”。“晋寒”的碾磨特性与“晋布”相似。在中部北部高地(注册号7318)的四个栽培地点,“金汉”的精米产量为5.43公吨/公顷。
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引用次数: 1
Crossing of Asiatic hybrids for breeding of new lily cultivars. 百合新品种选育的亚洲杂交种杂交。
Pub Date : 2018-03-01 DOI: 10.9787/KJBS.2018.50.1.1
Youngkwan Jo, F. Ramzan, Beung-Gu Son, Hong-Yul Kim, and Ki-Byung Lim
: This study was conducted to breed interspecific hybrids by crossing between Asiatic hybrids for the purpose of cut flower or pot plant. According to results of reciprocal crossing between L. davidii and ‘Conception’, flower color of F 1 appears nearer to that of each male parent but they seemed to be influenced by L. davidii which has side-facing flower position. The characteristic traits of F 1 hybrids obtained from the cross between ‘Tresor’ and ‘Black Out’ is related to female parent ‘Tresor’. Whereas in a cross between ‘Navidad’ and ‘Hironde’, a progeny 122611-4 has the petal spot characteristic similar to the female Asiatic hybrid ‘Navidad’ and male parent ‘Gironde’ influence on the petal color. F 1 hybrid cross ‘Gironde × Red Sensation’ showed parental flower color expression contribution. In addition ‘Gironde’ as parent contributed extensively in imparting flower color in the resulting hybrids. In the above statements, F 1 progenies were more than 40cm in height and the plant height of their parents was over 70cm. Therefore, it is considered that their F 1 progenies are not short if parents have a long plant height. In ‘Kotnali × Tiny Bee’ progenies, 112061-1 progeny expressed orange flower color similar to female while 112061-2 showed yellow flower color same as male. Plant height was 27.7cm and 22.4cm close to 35.0cm of male parent ‘Tiny Bee’. F 1 hybrid of ‘Matrix × Holebibi’ expressed three flower color all of parents. It was dwarf and distinguishable as compared to parental phenotype. Therefore, it is concluded that for potted lily flower development carrying dwarf plant height and intermediate morphological traits Asiatic hybrids plays an important role such as ‘Tiny Bee’ and ‘Matrix’.
本研究以切花或盆栽植物为目的,通过亚洲杂交种间杂交培育种间杂交种。结果表明,f1的花色与各父本的花色较为接近,但似乎受到花位朝侧的大花的影响。由‘Tresor’与‘Black Out’杂交获得的f1杂交种的特征性状与母本‘Tresor’有关。而在“Navidad”与“Hironde”的杂交中,后代122611-4具有与亚洲杂交母本“Navidad”相似的花瓣斑点特征,而父本“Gironde”对花瓣颜色的影响相似。杂交组合吉伦特×红感对亲本花颜色表达有贡献。此外,“吉伦特”作为亲本对杂交后代花色的影响也很大。以上陈述中,f1后代株高均在40cm以上,亲本株高均在70cm以上。因此,如果亲本株高较长,则认为其f1后代不矮。在“Kotnali × Tiny Bee”后代中,112061-1后代的花朵颜色与雌蜂相似,112061-2后代的花朵颜色与雌蜂相同,112061-2后代的花朵颜色与雌蜂相同。株高分别为27.7cm和22.4cm,接近父本“小蜂”的35.0cm。‘Matrix × Holebibi’杂种f1在所有亲本中均表现出三种花色。与亲本表型相比,它是矮化的,可区分的。综上所述,亚洲杂交品种如“小蜜蜂”和“矩阵”在盆栽百合花发育中起着重要的作用,具有矮化株高和中等形态性状。
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引用次数: 1
Variation of Isoflavone Contents and Classification Using Multivariate Analysis in Korean Soybean Varieties Released from 1913 to 2013 1913 ~ 2013年韩国大豆品种异黄酮含量变化及多变量分类分析
Pub Date : 2018-03-01 DOI: 10.9787/KJBS.2018.50.1.50
Hyun Myung Kim, Eun-Kyu Jang, B. Gwak, T. Hwang, Geon-Sig Yun, Se-Gu Hwang, Jeong, Heon-sang, H. Kim
This study was carried out to investigate the variation of 12 isoflavone components among 172 Korean soybean varieties released from 1913 to 2013. Cluster analysis was performed to classify the varieties based on the variation of isoflavone contents. Total isoflavone contents ranged from 206.3 μg/g to 2666.2 μg/g with an average of 837.2 μg/g. By each component, malonylgenistin content ranged from 99.2 μg/g to 1149.1 μg/g with the highest average of 390.0 μg/g, and glycitein content ranged from 0.1 μg/g to 2.4 μg/g with the lowest average of 0.8 μg/g. Among composition groups, the aglycone group contents ranged from 2.2 μg/g to 33.1 μg/g with the lowest average of 10.0 μg/g, and glucoside group contents ranged from 36.9 μg/g to 801.8 μg/g with an average of 202.4 μg/g. The malonylglucoside group contents ranged from 143.6 μg/g to 1796.4 μg/g with the highest average of 599.1 μg/g and acetylglucoside group contents ranged from 3.7 μg/g to 103.9 μg/g with an average of 27.5 μg/g. Varieties with high total isoflavone content were Aga8(2666.2 μg/g), Aga4(2569.3 μg/g), and Aga9(2345.0 μg/g) in bean sprouts group. The low total isoflavone content were observed from Deawang(328.8 μg/g) in soy sauce and paste group, Hanol(599.3 μg/g) in vegetable and early maturity group and Gemjeong1(664.1 μg/g) in cooking with rice group. The first three principal component axises accounted for 89.14% of the total variation observed. Dendrogram constructed using average linkage cluster method revealed that 172 Korean soybean varieties were divided into seven groups with the average distance of 0.8 between groups. The group I includes 57.6% of the total varieties, which was the largest group among seven groups, followed by the group II that includes 35.5% of the total varieties. However, the other groups were small groups containing less than five varieties. In comparison among the total isoflavone contents according to groups, the highest isoflavone content was observed in group VI with an average of 2526.9 μg/g, followed by group IV(1546.6 μg/g) and group V(1503.5 μg/g). The others were found to contain low isoflavone content.
本研究对1913 ~ 2013年发行的172个韩国大豆品种中12种异黄酮成分的变化进行了研究。根据异黄酮含量的变化进行聚类分析。总异黄酮含量为206.3 ~ 2666.2 μg/g,平均为837.2 μg/g。各组分丙二酸根素含量范围为99.2 ~ 1149.1 μg/g,最高平均值为390.0 μg/g;糖苷含量范围为0.1 ~ 2.4 μg/g,最低平均值为0.8 μg/g。各成分组中,糖苷组含量在2.2 ~ 33.1 μg/g之间,最低平均值为10.0 μg/g;葡萄糖苷组含量在36.9 ~ 801.8 μg/g之间,平均为202.4 μg/g。丙二烯基葡萄糖苷组含量为143.6 ~ 1796.4 μg/g,最高平均值为599.1 μg/g;乙酰葡萄糖苷组含量为3.7 ~ 103.9 μg/g,平均为27.5 μg/g。豆芽组总异黄酮含量较高的品种为Aga8(2666.2 μg/g)、Aga4(2569.3 μg/g)和Aga9(2345.0 μg/g)。酱油糊组的德旺(328.8 μg/g)、蔬菜及早熟组的汉醇(599.3 μg/g)、炒饭组的金晶1(664.1 μg/g)总异黄酮含量较低。前3个主成分轴占总变异量的89.14%。利用平均连锁聚类法构建的树状图显示,172个韩国大豆品种被划分为7个类群,类群之间的平均距离为0.8。第一类占品种总数的57.6%,是7个类群中数量最多的一类,其次是第二类,占品种总数的35.5%。然而,其他组是包含少于5个品种的小组。对各组总异黄酮含量进行比较,以ⅵ组最高,平均为2526.9 μg/g,其次为ⅳ组(1546.6 μg/g)和ⅴ组(1503.5 μg/g)。其他的被发现含有较低的异黄酮含量。
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引用次数: 4
Detection of QTLs in an Interspecific Cross between Perilla citriodora × P. hirtella Mapping Population 紫苏与hirtella定位群体种间杂交qtl的检测
Pub Date : 2018-03-01 DOI: 10.9787/KJBS.2018.50.1.13
M. Lee, K. Oh, M. Kim, Sung Up Kim, Jung-In Kim, Eunyoung Oh, S. Pae, U. Yeo, Tae-Ho Kim, Jeong Hee Lee, Chan-Sik Jung, D. Kwak, and Yong Chul Kim
An interspecific cross between P. citriodora and P. hirtella constitutes ideal material for a linkage map construction in genome project of Perilla. The chromosomes of the species are same with n=10 and progenies of the cross are normal in growth and seed set. The phenotype of F2 population of the cross are normally distributed and this is a proof of high affinity of the chromosomes during their sexual reproduction. Rosmarinic acid, Luteolin and Apigenin contents of F2 plants were distributed in similar range with tetraploid perilla cultivars. Luteolin and Apigenin are positively correlated with correlation coefficient of 0.762. 21 QTLs were detected in agronomic traits and Rosmarinic acid, Luteolin and Apigenin contents. Even though Luteolin and Apigenin are positively correlated, QTLs were located in different position. Purple leaf color related QTL was mapped in Chromosome 3 with LOD of 14.3, PVE of 50.4%. Three anthocyanin biosynthesis transcription factor like sequences, WD40 repeat-like superfamily protein, myb domain protein 43 and basic helix-loop-helix (bHLH) DNA-binding superfamily protein, were detected near from the QTL.
柠檬酸假单胞菌与hirtella假单胞菌的种间杂交为紫苏基因组计划中连锁图谱的构建提供了理想的材料。在n=10时,两种染色体相同,杂交后代生长和结实率正常。杂交F2群体的表型呈正态分布,这证明了染色体在有性繁殖过程中具有很高的亲和力。F2植株迷迭香酸、木犀草素和芹菜素含量与四倍体紫苏品种分布范围相近。木犀草素与芹菜素正相关,相关系数为0.762。农艺性状和迷迭香酸、木犀草素、芹菜素含量共检测到21个qtl。尽管木犀草素与芹菜素呈正相关,但qtl定位位置不同。在第3染色体上定位紫色叶片颜色相关QTL, LOD为14.3,PVE为50.4%。在QTL附近检测到3个花青素生物合成转录因子样序列,即WD40重复样超家族蛋白、myb结构域蛋白43和碱性螺旋-环-螺旋(bHLH) dna结合超家族蛋白。
{"title":"Detection of QTLs in an Interspecific Cross between Perilla citriodora × P. hirtella Mapping Population","authors":"M. Lee, K. Oh, M. Kim, Sung Up Kim, Jung-In Kim, Eunyoung Oh, S. Pae, U. Yeo, Tae-Ho Kim, Jeong Hee Lee, Chan-Sik Jung, D. Kwak, and Yong Chul Kim","doi":"10.9787/KJBS.2018.50.1.13","DOIUrl":"https://doi.org/10.9787/KJBS.2018.50.1.13","url":null,"abstract":"An interspecific cross between P. citriodora and P. hirtella constitutes ideal material for a linkage map construction in genome project of Perilla. The chromosomes of the species are same with n=10 and progenies of the cross are normal in growth and seed set. The phenotype of F2 population of the cross are normally distributed and this is a proof of high affinity of the chromosomes during their sexual reproduction. Rosmarinic acid, Luteolin and Apigenin contents of F2 plants were distributed in similar range with tetraploid perilla cultivars. Luteolin and Apigenin are positively correlated with correlation coefficient of 0.762. 21 QTLs were detected in agronomic traits and Rosmarinic acid, Luteolin and Apigenin contents. Even though Luteolin and Apigenin are positively correlated, QTLs were located in different position. Purple leaf color related QTL was mapped in Chromosome 3 with LOD of 14.3, PVE of 50.4%. Three anthocyanin biosynthesis transcription factor like sequences, WD40 repeat-like superfamily protein, myb domain protein 43 and basic helix-loop-helix (bHLH) DNA-binding superfamily protein, were detected near from the QTL.","PeriodicalId":448090,"journal":{"name":"Korean Journal of Breeding","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133274668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Development of a Late Maturing Citrus Cultivar, ‘Tamdosamho’ 晚熟柑桔品种‘淡多samho’的选育
Pub Date : 2018-03-01 DOI: 10.9787/KJBS.2018.50.1.61
Sukman Park, S. Yun, S. Koh, Dong-Hoon Lee, Min Ju Kim, Young Hun Choi, and Jae Ho Park
: A new citrus cultivar ‘Tamdosamho’ is a hybrid obtained from a cross of ‘Kiyomi’ ( Citrus unshiu × Citrus sinensis , the seed parent) and ‘Sunburst’ ( Citrus reticulate , the pollen parent) at the Citrus Research Institute of the National Institute of Horticultural and Herbal Science in 2004. ‘Tamdosamho’ had the first fruit set in 2010 and field evaluation has been conducted until 2012. The tree of 'Tamdosamho' was vigorous and had a lot of fruit bearing. The average fruit weight was 230g and its shape was flatten-oblate with a little neck. The fruit peel color was dark orange, and peel coloration began in mid-October and fully developed in early December. The fruit matured in mid-February from cultivation under plastic film house. Total soluble solid and acidity of which was 14.0 °Brix and about 1 % respectively. The thickness of the rind was about 2.5 mm on average and was thinner than that of satsuma mandarin ( Citrus unshiu ), so removing the peel was easy. This cultivar had characteristic of self-incompatibility and parthenocarpy and didn’t bear seeds from monoculture but if it be fertilized by the pollen of other cultivars could be seedy. In the case of cultivation in the open field, the fruits could be damaged by cold temperature and then ‘Tamdosamho’ is recommended for cultivation under plastic film house. (Variety registration number: 6158)
柑橘新品种“Tamdosamho”是2004年国立园艺和草药科学研究所柑橘研究所的“Kiyomi”(citrus unshiu × citrus sinensis,种子亲本)和“Sunburst”(citrus reticulate,花粉亲本)杂交而成的新品种。“Tamdosamho”于2010年首次结果,并在2012年之前进行了实地评价。“Tamdosamho”的树很健壮,结果很多。果实平均重230g,形状扁扁,有小颈。果皮颜色为深橙色,10月中旬开始果皮变色,12月初完全发育。2月中旬在塑料薄膜棚内栽培成熟。总可溶性固形物为14.0°白利度,酸度为1%左右。果皮厚度平均约2.5 mm,比蜜桔薄,去皮容易。该品种具有自交不亲和孤雌核的特点,单栽不结籽,但与其他品种的花粉受精后可结籽。如果在露天栽培,果实可能受到低温的损害,建议在塑料薄膜棚内栽培“Tamdosamho”。(品种注册号:6158)
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引用次数: 0
Diallel Analysis of Agronomic and Palatability Traits in Waxy Corn Hybrids from Korean Landraces 韩国地方糯玉米杂交种农艺性状和适口性状的双列分析
Pub Date : 2018-03-01 DOI: 10.9787/KJBS.2018.50.1.33
Seung-hyun Wang, J. Chung, Min-Jeong Kang, W. Shim, and Yoon-Sup So
: This study was carried out to select waxy corn inbreds with good general combining ability through a diallel analysis of 10 half diallel crosses among five inbreds developed from Korean maize landraces. Total of 21 agronomic and palatability traits were investigated over two locations in 2016. There was a significant hybrid by location interaction found in plant height, ear height, number of tillers/plant, per ear weight, ear tip filling, ear commercial value and sweetness when four additional check cultivars were included in the analyses. A significant general combining ability by location interaction was found in ear height, number of tillers/plant, per ear weight, ear length and sweetness while ear width, ear commercial value and sweetness were significant for specific combining ability by location interaction. Sweetness was found significant for both interactions. The first and fourth inbred lines had the most number of traits with good estimate of general combining ability. The first inbred is expected to improve days to tasseling, anthesis silking interval, ear width, ear circumference and sweetness while decreasing husk cover in a hybrid combination. The fourth inbred line would improve days to tasseling, days to silking, plant height, ear height, per ear weight and ear length but ears would be a bit tougher than the average. A total of 19 traits had higher variance of general combining ability than that of specific combining ability. Husk cover was 12.1 times higher. Plant height and number of tillers/plant had higher variance of specific combining ability.
本研究通过对韩国玉米地方品种自交系5个自交系10个半双列杂交的双列分析,筛选一般配合力较好的糯玉米自交系。2016年在两个地点共调查了21个农艺和适口性状。另外4个对照品种在株高、穗高、分蘖数、穗重、穗尖灌浆、穗商业价值和甜度等方面存在显著的位置互作效应。位置互作对穗高、分蘖数、穗重、穗长和甜度的一般配合力显著,对穗宽、穗商业价值和甜度的特殊配合力显著。甜味对两种相互作用都很重要。自交系1号和4号性状数量最多,一般配合力估计较好。在杂交组合中,首个自交系可望提高抽雄天数、花丝间隔、穗宽、穗围和甜度,同时减少壳盖。第4个自交系可以提高抽雄天数、出丝天数、株高、穗高、每穗重和穗长,但穗粗略高于平均水平。共有19个性状的一般配合力方差高于特定配合力方差。壳盖高了12.1倍。株高和分蘖数比配合力变异较大。
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引用次数: 0
Breeding of Phalaenopsis ‘SM 7661’ with Mini Type of Dark Red Flower 暗红色小花型蝴蝶兰SM 7661的选育
Pub Date : 2018-03-01 DOI: 10.9787/KJBS.2018.50.1.65
K. Lim, Hong-Yul Kim, N. Park, and Beung Gu Son
: A new Phalaenopsis cultivar SM 7661 was bred by Kyungpook National University, Korea. The new cultivar SM 7661 with the red colored mini type characteristics, was derived by artificial crossing between Phal. Golden Sun(female parent) and Phal. Princess Kaiulani(male parent). From 2005 to 2006, 250 individuals were cultivated over two years. Among them, an elite individual number '03-6-61' with excellent growth and flowering characteristics was finally selected by intensive selection process. The stability and uniformity of the cultivar was confirmed through the first and second characteristics tests in 2007 and 2008 and named as 'SM 7661'. SM 7661 produces dark red (RHS#185B) flowers of round shape and spreading mini type with size of 5.7 and 5.8cm in flower length and width, respectively. SM 7661 is regarded as raceme flower type with many flowers and good volume and suitable for small flower pot market. The leaf of SM 7661 is rowing horizontally and 13.8cm in length and 7.2cm in width. This cultivar also possesses no genetic variation, fast in vitro propagation and easy growing due to vigorous growth habit. This SM 7661 is registered (Reg.#:3826)to Korea Seed & Variety Service(KSVS) in December 21, 2011 and PBR(plant breeder's right) is currently controlled by Sangmiwon Orchid Company, Korea.
:庆北大学选育蝴蝶兰新品种SM 7661。通过人工杂交获得了具有红色迷你型性状的新品系SM 7661。金太阳(母本)和帕尔。凯乌拉尼公主(父亲)。从2005年到2006年,两年多培养了250株。通过集约选育,最终获得了一株生长和开花性状优良的优良单株‘03-6-61’。通过2007年和2008年的第一次和第二次性状试验,确定了该品种的稳定性和均匀性,并命名为“SM 7661”。SM 7661的花朵呈深红色(RHS#185B),呈圆形,花长5.7厘米,花宽5.8厘米。SM 7661被认为是总状花序型,花多,体积好,适合小花盆市场。SM 7661的叶片水平划船,长13.8厘米,宽7.2厘米。该品种无遗传变异,生长习性旺盛,离体繁殖快,易生长。该SM 7661于2011年12月21日在韩国种子和品种服务局(KSVS)注册(注册号:3826),PBR(植物育种者的权利)目前由韩国Sangmiwon Orchid公司控制。
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引用次数: 0
Identification of Root-specific Promoters Derived from Arabidopsis thaliana 拟南芥根特异性启动子的鉴定
Pub Date : 2018-03-01 DOI: 10.9787/KJBS.2018.50.1.21
Jin Sun Kim, Sun-Hyung Lim, Sang-Ho Kang, Young-Mi Kim, and Jong-Yeol Lee
: To identify and apply tissue-specific promoters is one of the major challenges in plants genetic engineering for optimizing efficient expression of interest genes in appropriate tissues. In this research, open-source database information of Arabidopsis thaliana was adapted to determine root-specific expressed promoter region. A total seven sequences that might function as a root-specific promoter element were initially isolated from Arabidopsis genomic DNA. Then seven promoters were cloned into pBGWFS7 in which β -glucuronidase ( gus ) and green fluorescent protein (gfp) genes were linked. The GUS activities were measured in different tissues of transgenic Arabidopsis by both histochemical GUS staining and fluorescent 4 methylumbelliferyl β -D-glucuronide (MUG) assay. To confirm root-specific expression, GFP-confocal microscope analysis was conducted in Arabidopsis transgenic plant. As a result, the five promoters showed strong GUS activity in the root tissue as compared with the CaMV35S promoter. To test crop application availability as a root-specific promoter, seven promoters were introduced into tomato plants and confirmed transient expression using Agrobacterium rhizogenesis ARqua1 root-nodule inducible strain. Two promoters showed that gus genes were specifically expressed in roots of transgenic tomato plant. Taken together, the novel seven promoters showed specific activity in root suggesting that it is applicable in crop improvement.
组织特异性启动子的鉴定和应用是植物基因工程的主要挑战之一,目的是优化相关基因在合适组织中的高效表达。本研究利用拟南芥的开源数据库信息来确定根特异性表达启动子区域。从拟南芥基因组DNA中分离出7个可能作为根特异性启动子元件的序列。将7个启动子克隆到pBGWFS7中,其中β -葡萄糖醛酸酶(gus)和绿色荧光蛋白(gfp)基因在pBGWFS7中连接。采用组织化学GUS染色法和4 methylumbellliferyl β -D-glucuronide (MUG)荧光法测定转基因拟南芥不同组织中GUS的活性。为了确认根特异性表达,在转基因拟南芥植株上进行了gfp共聚焦显微镜分析。结果表明,与CaMV35S启动子相比,这5个启动子在根组织中表现出较强的GUS活性。为了验证作为根特异性启动子的作物应用有效性,将7个启动子引入番茄植株,并利用根结农杆菌ARqua1根结诱导菌株进行瞬时表达。两个启动子表明gus基因在转基因番茄植株的根中特异性表达。结果表明,这7个启动子在根系中具有特异活性,可用于作物改良。
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引用次数: 1
Environmental Variation of Taxifolin Content in a Rice Variety ‘Superhongmi’ 水稻品种‘Superhongmi’杉油素含量的环境变异
Pub Date : 2018-03-01 DOI: 10.9787/KJBS.2018.50.1.45
S. Ryu
This study was conducted to obtain general information for rice breeding materials high in taxifolin content. The taxifolin was isolated from the rice variety, ‘Superhongmi’, and was identified by means of spectral methods and quantitative analysis using a high performance liquid chromatography (HPLC). The taxifolin content of the ‘Superhongmi’ variety was ranged between 32mg and 68mg per 100g seed that differs depending on cultivated regions. The taxifolin content was lower grown at mid north than those at south region. A large amount of taxifolin was found in the aleurone layer of seed as well as in the node of spike of the ‘Superhongmi’. The weight of 1,000 grains was ranged between 23.5g and 27.8g that varied depending on cultivated regions. This variety showed heading in the 6 th of September, and its fertility ratio was about 83% in sejong region. These results suggest that the taxifolin-rich rice variety, ‘Superhongmi’, is a good source of functional and stable food.
本研究旨在获得杉木素含量高的水稻育种材料的一般资料。从水稻品种‘Superhongmi’中分离得到一种taxifolin,并采用高效液相色谱(HPLC)和光谱分析方法对其进行了鉴定。“超级红米”品种的紫杉醇含量在每100克种子32毫克到68毫克之间,根据种植地区的不同而不同。中北部地区杉木素含量低于南部地区。在种子糊粉层和穗节中发现了大量的紫杉素。1000粒谷物的重量在23.5克到27.8克之间,根据种植地区的不同而不同。该品种于9月6日抽穗,在世宗地区产仔率达83%左右。这些结果表明,富含紫杉醇素的水稻品种“Superhongmi”是一种功能稳定的良好食物来源。
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引用次数: 2
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Korean Journal of Breeding
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