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REDUCING OF AMMONIA AND HYDROGEN SULFIDE EMISSION FROM CHICKEN LITTER BY THE USE OF INORGANIC AND BIOLOGICALLY ACTIVE SUBSTANCES 利用无机和生物活性物质减少鸡粪中氨和硫化氢的排放
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl2023229
M. Vorobel, Oleh Klym, V. Kaplinskyi, Oksana Nykyforuk, Viktoriia Momut, Stepan Grabovskyi, Natalia Lopotych, Oleksandra Grabovska, Halyna Denys, H. Bilovus
The accumulation of chicken litter, due to the excessive emission of gaseous air pollutants, causes a significant load on the environment. Therefore, the basis of research was to find out the level of NH3 and H2S emission from chicken litter by the influence of inorganic and biologically active substances. It is established that the lowest level of NH3 release from litter - by 25.5-32.6% and H2S by 19.7-24.4% is observed with the introduction of a complex preparation for deactivation of enzymatic processes in chicken litter. Of the studied natural sorbents, the best results for reducing NH3 and H2S emission from the litter were obtained by the use of vermiculite - by 24.4-28.7% and 12.6-15.9%. The biopreparation Kapeliukhiv Yarok more effectively affects the reduction of the NH3 release from the substrate (24.3-27.7%) and the Scarabei - H2S (16.1-19.2%). The introduction in chicken litter magnesium acetate and superphosphate helps to reduce NH3 emission by 22.2-22.8% and 22.3- 24.3%, while H2S by 16.0-19.2% and 16.3-17.2%.
由于气态空气污染物的过量排放,鸡粪的堆积对环境造成了巨大的负荷。因此,研究的基础是在无机和生物活性物质的影响下,找出鸡粪中 NH3 和 H2S 的排放水平。研究结果表明,在使用一种复合制剂使鸡粪中的酶过程失活后,鸡粪中的 NH3 排放量最低,为 25.5-32.6%,H2S 排放量最低,为 19.7-24.4%。在所研究的天然吸附剂中,使用蛭石减少鸡粪中 NH3 和 H2S 排放的效果最好,分别减少了 24.4-28.7% 和 12.6-15.9%。Kapeliukhiv Yarok 生物制剂能更有效地减少基质中的 NH3 排放量(24.3-27.7%)和 Scarabei - H2S 排放量(16.1-19.2%)。在鸡粪中引入醋酸镁和过磷酸钙有助于减少 22.2-22.8% 和 22.3-24.3% 的 NH3 排放,以及 16.0-19.2% 和 16.3-17.2% 的 H2S 排放。
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引用次数: 0
STUDY OF PHENOTYPIC AND GENETIC VARIABILITY IN MAIZE CROSSINGS BETWEEN CYCLE I INBRED LINES AND ELITE LINES (CYCLE II) 周期 I 近交系与精英系(周期 II)杂交玉米的表型和遗传变异研究
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl2023227
Carmen Vana, Andrei Varga, R. Călugăr, Ancuta Ceclan, N. Tritean, A. Tărău
Maize inbred lines derived from local populations (Cycle I lines) were tested using cycle II elite inbred lines. In the cross system, hybrids have a wide variability for yield (6601-10571 kg/ha), the highest differences between hybrids are due to the non-additive genetic effects; undesirable traits transmitted by cycle I inbred lines are weak resistance to stalk breaking and fallen plants; for the percentage of unbroken plants, the genetic variability is high, the percentage of unbroken plants for simple hybrids has a range value between 65.02% and 89.70%.There were calculated the correlations coefficients between ”per se” values for the correlation of inbred lines from crossbreeding system and additive genetic effects values calculated in the crossbreeding system ”m x n”. Positive correlations were identified between the values per se and the positive genetic effects for yield, ear weight, ear length, numbers of kernels per ear, TKW, kernel depth. These high positive correlation values indicate the possibility to use phenotypic markers in the process of inbred lines creation.
玉米近交系来自当地种群(周期 I 系),并与周期 II 的优良近交系进行了测试。在杂交系统中,杂交种的产量差异很大(6601-10571 千克/公顷),杂交种之间的最大差异是由于非加性遗传效应造成的;周期 I 近交系传递的不良性状是对茎秆折断和倒伏的抗性较弱;未折断植株百分比的遗传变异较大,简单杂交种的未折断植株百分比范围值在 65.02% 到 89.70% 之间。计算了杂交系统近交系相关性 "本身 "值与杂交系统 "m x n "计算的遗传效应加值之间的相关系数。在产量、穗重、穗长、每穗籽粒数、TKW 和籽粒深度方面,发现 "本身 "值与正遗传效应值之间存在正相关。这些高正相关值表明,在创建近交系的过程中可以使用表型标记。
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引用次数: 0
ANTIFUNGAL ACTIVITY OF Pediococcus pentosaceus ISOLATED FROM BAMBARA GROUNDNUT (Vigna subterranea (L.) Verdc.) SEEDS AGAINST Aspergillus flavus 从巴姆巴拉花生(Vigna subterranea (L.) Verdc.)种子中分离的五味子球菌对黄曲霉的抗菌活性
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl2023217
Amidou S. Ouili, C. Diguță, Y. Maiga, Cheik Omar Tidiane Compaoré, A. Ouattara, Florentina Israel-Roming, F. Matei
In this study, 22 lactic acid bacterial (LAB) strains were isolated from Bambara groundnut seeds and assessed for their ability to suppress the development of two strains of aflatoxigenic Aspergillus flavus. The antifungal activity of LAB strains was assessed via the overlay assay. Also, HPLC methodology was used to quantify the production of lactic and acetic acid by the LAB strains with high antifungal activity. After incubation at 30°C for 72 hours, five of these LAB strains (52B1, 52C1, 74B3, 67B1, 67A9) showed high inhibitory activity against Aspergillus flavus strains. These LAB strains exhibiting antifungal potential were identified as Pediococcus pentosaceus through 16S rDNA sequencing. Furthermore, the lactic acid bacteria (LAB) strains reported lactic acid production ranging from 12.08 mg/ml to 15.38 mg/ml and acetic acid production ranging from 0.06 mg/ml to 1.68 mg/ml. Notably, antifungal experiments are conducted in a controlled setting. Further, it would be worthwhile to explore the direct application of Pediococcus pentosaceus strains or their antimicrobial agents to food products to prevent fungal growth during storage. By doing so, the use of fungicides can be avoided and lead to safer food products.
本研究从班巴拉落花生种子中分离出 22 株乳酸菌(LAB),并评估了它们抑制两种黄曲霉菌株发展的能力。通过重叠试验评估了 LAB 菌株的抗真菌活性。此外,还采用高效液相色谱法对具有高抗真菌活性的 LAB 菌株产生的乳酸和乙酸进行了量化。在 30°C 下培养 72 小时后,其中五株 LAB 菌株(52B1、52C1、74B3、67B1 和 67A9)对黄曲霉菌株表现出较高的抑制活性。通过 16S rDNA 测序,这些具有抗真菌潜力的 LAB 菌株被鉴定为五胜肽球菌。此外,乳酸菌(LAB)菌株的乳酸产量为 12.08 毫克/毫升至 15.38 毫克/毫升,醋酸产量为 0.06 毫克/毫升至 1.68 毫克/毫升。值得注意的是,抗真菌实验是在受控环境下进行的。此外,值得探讨的是将五胜肽球菌菌株或其抗菌剂直接应用于食品,以防止真菌在贮存期间生长。这样就可以避免使用杀真菌剂,从而生产出更安全的食品。
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引用次数: 0
FORMS OF FERRUM IN PROFILE-DIFFERENTIATED SOILS OF PRECARPATHIAN UKRAINE AND THEIR ROLE IN DIAGNOSTICS OF SOIL-FORMING PROCESSES 乌克兰前喀尔巴阡剖面差异土壤中铁的形态及其在土壤形成过程诊断中的作用
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl2023219
Zenoviy Pankiv, Olena Kalynych, T. Yamelynets, Oksana Bonishko
The profiles of sod-podzolic surface-gleyed soils (Stagnic Retisols) and brown podzolic soil profile (Neocambic Gleyic Retisols) are characterized by differentiation of gross Ferrum content and moderately low degree of Ferrum content. The composition of gross Ferrum is dominated by Fes, the maximum share of which is characteristic of A2B g horizon. The minimum content of Fes belongs to A1A2 g, which indicates the processes of destruction of primary and secondary minerals in the upper part of the profile. The accumulation of Ferrum oxides was diagnosed in the ortstein and nodules of the studied soils. The Schwertman coefficient in the neoplasms of the over-illuvial part of the profile is 0.6-0.7, which confirms the theory of their formation under the predominant action of the gleyish-eluvial process. The slight influence of modern gleiing process and their relict origin are observed in transition horizon.
草皮荚膜表层沟壑土壤(Stagnic Retisols)和棕色荚膜土壤(Neocambic Gleyic Retisols)的剖面具有总铁含量分化和铁含量中等偏低的特点。毛铁的成分以铁元素为主,A2B g 层的铁元素含量最高。A1A2 g 地层的铁含量最低,这表明剖面上部存在原生和次生矿物的破坏过程。在所研究土壤的褶皱层和结核中发现了氧化亚铁的积累。剖面上冲积层部分新生物的施韦尔曼系数为 0.6-0.7,这证实了它们是在主要的砾岩-冲积过程作用下形成的理论。在过渡地层中可以观察到现代砾岩化过程的轻微影响和它们的孑遗起源。
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引用次数: 0
SEROLOGICAL SCREENING FOR Neospora caninum INFECTION IN A DAIRY CATTLE FARM WITH REPRODUCTIVE DISORDERS, IN SOUTH-EASTERN ROMANIA 在罗马尼亚东南部一个患有生殖系统疾病的奶牛场进行新孢子菌感染血清学筛查
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl2023210
Vasilica Gotu, Emanuel Mitrea, D. Porea, M. Ioniță, I. Mitrea
Neospora caninum is an apicomplexan protozoa parasite that causes reproductive disorders in cattle worldwide. Despite the high seroprevalence reported in Romania, there are limited data on N. caninum infection in dairy cattle, and no studies have evaluated the presence of infection over time in a limited area. Therefore, this study aimed to assess the seroprevalence of N. caninum in a dairy cattle farm in Southeastern Romania where a previously study reported about the cattle exposure to N. caninum infection. A total of 85 cattle, assigned in four groups, according to the reproductive status (repeated breeding syndrome, history of abortion, pregnant cows, and fresh cows) were tested for the presence of N. caninum antibodies, using an indirect ELISA test. The overall seroprevalence was 28.23% (95% CI: 18.67–37.80). According to the reproductive groups, the seroprevalence varied between 19.04% (95% CI: 2.25-35.84) in fresh cows to 38.09% (95% CI: 17.32 - 58.87) in cows with repeated breeding syndrome. By these findings new information is provided about the disease's presence in the studied farm, implying that infection with N. caninum may be the primary cause of reported reproductive disorders, as well as about the infection's persistent nature.
犬新孢子虫(Neospora caninum)是一种 apicomplexan protozoa 寄生虫,可导致全球牛只的繁殖障碍。尽管罗马尼亚的血清流行率很高,但有关奶牛感染犬新孢子虫的数据却很有限,也没有任何研究对有限地区的长期感染情况进行评估。因此,本研究旨在评估罗马尼亚东南部一家奶牛场的犬银环蛇血清流行率,此前曾有研究报告称该地区的奶牛感染了犬银环蛇。根据繁殖状况(重复配种综合症、流产史、怀孕奶牛和新产奶牛)将总共 85 头牛分成四组,使用间接 ELISA 检测法检测是否存在犬鼻疽抗体。总体血清阳性率为 28.23%(95% CI:18.67-37.80)。根据繁殖组别,血清流行率从新产奶牛的 19.04% (95% CI: 2.25-35.84) 到重复繁殖综合症奶牛的 38.09% (95% CI: 17.32 - 58.87)不等。这些研究结果提供了有关该疾病在所研究牧场存在情况的新信息,暗示感染犬疫母蚤可能是所报告繁殖障碍的主要原因,以及感染的持续性。
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引用次数: 0
DEMOGRAPHIC CHARACTERIZATION AND CONSUMPTION HABITS OF PORTUGUESE HUNTERS 葡萄牙猎人的人口特征和消费习惯
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl202321
A. Abrantes, Ana Raquel FERNANDES, M. Vieira-Pinto
Hunting is the most popular rural activity in Portugal. Currently, the number of licensed hunters in Portugal is around 115 000, preferentially hunting large game, particularly wild boar. The objective of this study is the demographic characterization of the hunters who consume game meat. In an ongoing anonymous survey of hunters on `wild boar consumption habits`,were raised questions about personal demographic data (age, gender, scholar level, and residency) and wild boars’ meat self-consume, such as the number of hunted animals per season and their self-consume and if, they consume it undercooked or make traditional products, as sausages. Of the 206 hunters from all over Portugal who responded to the survey, the majority are males aged between 31 and 50 years (n = 106; 51.5%) and practice selfconsumption of wild boar meat they hunt (average wild boar hunted per hunting season is 4). 177 do not make traditional products based on raw game meat (86%), nor do they consume it undercooked. Knowing these data is important to understand the attributable risk of self-consuming game meat and the perception of hunters about this.
狩猎是葡萄牙最受欢迎的农村活动。目前,葡萄牙持证狩猎者的数量约为 115 000 人,他们喜欢狩猎大型野味,尤其是野猪。这项研究的目的是了解食用野味的猎人的人口特征。在对猎人进行的一项关于 "野猪肉消费习惯 "的匿名调查中,提出了有关个人人口数据(年龄、性别、学历和居住地)和野猪肉自我消费的问题,如每季狩猎野猪的数量和自我消费情况,以及他们是否食用未煮熟的野猪肉或制作香肠等传统产品。来自葡萄牙各地的 206 名猎人对调查做出了回应,其中大多数是 31 至 50 岁的男性(n = 106;51.5%),他们对猎获的野猪肉进行自我消费(每个狩猎季节平均猎获 4 头野猪)。177 人不制作以生野猪肉为原料的传统产品(86%),也不食用未煮熟的野猪肉。了解这些数据对于了解自行食用野味的可归属风险以及猎人对此的看法非常重要。
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引用次数: 0
SCREENING FOR A SUITABLE CRYOPRESERVATION PROTOCOL FOR Polypodium vulgare 筛选合适的多浆植物晶体保存方案
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl202327
R. Catană, Florentina Aldea, Monica Mitoi
Cryopreservation (storage at -196°C) is one of the techniques used for ex situ conservation to guarantee the long-term storage of different biological materials. Polypodium vulgare (Common polypody) is an evergreen fern used as food and medicine. To our knowledge, no cryopreservation protocol for gametophytes from this species is available. In this context, our paper aimed to screen a suitable cryopreservation protocol for P. vulgare - an important source of secondary metabolites. Different protocols were tested to identify a suitable cryopreservation protocol. Techniques like light microscopy and biochemical assays were used to certify an appropriate protocol for this fern. Gametophyte desiccation for 3 hours in the sterilized airflow before rapid immersion in liquid nitrogen provides sufficient dehydration to assure a good rate of survival.
低温保存(零下 196 摄氏度)是用于异地保存的技术之一,可确保不同生物材料的长期储存。多肉植物(Polypodium vulgare)是一种常绿蕨类植物,可用作食物和药物。据我们所知,目前还没有针对该物种配子体的低温保存方案。在这种情况下,我们的论文旨在筛选一种适用于 P. vulgare 的低温保存方案--P. vulgare 是次生代谢物的重要来源。我们对不同的方案进行了测试,以确定合适的低温保存方案。使用光学显微镜和生化检测等技术来验证适合这种蕨类植物的方案。先将配子体在灭菌气流中干燥 3 个小时,然后快速浸入液氮,这样可以充分脱水,确保良好的存活率。
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引用次数: 0
EVALUATION OF SEED YIELD AND THOUSAND SEED WEIGHT OF CONFECTIONERY SUNFLOWER (Helianthus annuus L.) VARIETIES GROWN BY DIFFERENT FARMING TECHNIQUES IN THE NORTHERN STEPPE OF UKRAINE 在乌克兰东北部地区采用不同耕作技术种植的葵花(Helianthus annuus L.)品种的籽粒产量和千粒重的评价
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl2023215
K. Makliak, M. Korkodola
The paper presents data on the seed yield and thousand seed weight of confectionery sunflower (Helianthus annuus L.) varieties cultivated in the Northern Steppe of Ukraine. The study was conducted on three varieties-populations and one F1 hybrid. The study design included two types of basic tillage, three doses of fertilizer, and three plant densities. The seed yield significantly (P = 0.05) depended on the year of testing (the contribution of the year factor to the total variability was 38.88%), plant density (29.30%) and fertilizer dose (14.43%). It also depended on the tillage type (3.01%) and varietal characteristics (0.87%). The highest yield of seeds (up to 4.24 t/ha) was obtained with moldboard plowing, NPK fertilizer doses of 40:60:60 and 60:80:80 and plant density of 40,800 plants/ha. The thousand seed weight significantly (P=0.05) changed under the influence of the year of testing (32.11%), plant density (26.75%) and variety (25.92%), as well as under the influence of fertilization (3.24%) and tillage (3.75%). The largest thousand seed weight (up to 122.3 g) was obtained with moldboard plowing, NPK fertilizer doses of 40:60:60 and 60:80:80 and plant density of 20,400 plants/ha. Recommendations on the farming technique for confectionery sunflower have been drawn up.
本文介绍了在乌克兰北部草原种植的向日葵(Helianthus annuus L.)品种的种子产量和千粒重数据。该研究针对三个品种和一个 F1 代杂交种进行。研究设计包括两种基本耕作方式、三种肥料剂量和三种植株密度。种子产量明显(P = 0.05)取决于试验年份(年份因素对总变异的贡献率为 38.88%)、植株密度(29.30%)和肥料剂量(14.43%)。此外,还取决于耕作类型(3.01%)和品种特性(0.87%)。模耕、氮磷钾肥剂量为 40:60:60 和 60:80:80、植株密度为 40 800 株/公顷时,种子产量最高(达 4.24 吨/公顷)。千粒重在试验年份(32.11%)、植株密度(26.75%)和品种(25.92%)以及施肥(3.24%)和耕作(3.75%)的影响下发生了显著变化(P=0.05)。采用模板耕地、氮磷钾肥剂量为 40:60:60 和 60:80:80、种植密度为 20,400 株/公顷时,种子千粒重最大(达 122.3 克)。已就糖果向日葵的种植技术提出建议。
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引用次数: 0
STRUCTURAL EFFECTS INDUCED BY CULTIVATION CONDITIONS IN Momordica charantia L. PLANTS 栽培条件对 Momordica charantia L. 植物结构的影响植物
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl2023221
A. Sarbu, A. Cîșlariu, T. Șesan, A. Paraschiv, Daniela Clara Mihai
Momordica charantia L. (Cucurbitaceae), a nutraceutical plant, was acclimatized in Romania in 1990. It is cultivated in the greenhouses of S.C. HOFIGAL Import Export S.A., Romania, both in classical conditions, for commercial purposes, and in various experimental conditions, for increasing the medicinal value and the productivity of the taxon. The present study refers to the Momordica charantia plants, grown in experimental conditions, in soil enriched with porous ceramic granules and aims to identify beneficial structural changes that favor their better development. The histo-anatomical observations concerned the organization of the stem, petiole, leaf lamina and tendrils. The structural characteristics were compared to those of plants grown in the absence of ceramic granules. Our results highlight dimensional increases in the vegetative organs of plants cultivated in the presence of ceramic granules, significantly higher development of conductive tissues, assimilating tissue, stomatal index and foliar cystoliths, beneficial changes for plant growth and development.
Momordica charantia L.(葫芦科)是一种营养保健植物,于 1990 年在罗马尼亚种植。它在罗马尼亚 S.C. HOFIGAL Import Export S.A. 公司的温室中栽培,既有传统条件下的商业栽培,也有各种实验条件下的栽培,以提高该分类群的药用价值和产量。本研究指的是在实验条件下,在富含多孔陶瓷颗粒的土壤中生长的 Momordica charantia 植物,旨在确定有利于其更好发展的有益结构变化。组织解剖学观察涉及茎、叶柄、叶片和卷须的组织。我们将这些结构特征与没有使用陶粒的植物进行了比较。我们的研究结果表明,使用陶粒栽培的植物无性器官尺寸增加,传导组织、同化组织、气孔指数和叶片囊石的发育显著提高,这些都是有利于植物生长和发育的变化。
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引用次数: 0
WINTER WHEAT MUTATION VARIABILITY UNDER LOW-DAMAGE ABILITY MUTAGEN ACTION 低损伤能力诱变剂作用下冬小麦突变的变异性
IF 0.5 Q4 AGRONOMY Pub Date : 2023-12-31 DOI: 10.17930/agl2023212
V. Horshchar, M. Nazarenko
The purpose of our study was to show the possibilities of a chemical supermutagen in the regular induction of certain types of mutations. Winter wheat dry seeds of eight varieties were acted with water (control) and DAB (1.4-bisdiazoacetylbutane) action in concentrations of 0.1%, 0.1%, 0.3%. Studies have shown that the use of this mutagen is expedient from the point of view of obtaining valuable forms of low-growing, earlyripening, late-ripening, high-yielding lines, forms with high productive tillering. The mutagen by its nature has a rather low affinity, at least for this group of genotypes, however, this factor still affects the frequency of some types of mutations. Even extreme concentrations of this mutagenic agent did not lead to a significant decrease in the number of mutations; however, they reached a maximum in the spectrum of possible changes. Further studies are needed on changes in photosynthetic activity and associated persistence.
我们研究的目的是显示化学超诱变剂在定期诱导某些类型突变方面的可能性。八个品种的冬小麦干种子分别与水(对照)和浓度为 0.1%、0.1%、0.3% 的 DAB(1.4-双偶氮乙酰丁烷)作用。研究表明,从获得有价值的低矮型、早熟型、晚熟型、高产型、高产分蘖型品种的角度来看,使用这种诱变剂是很方便的。诱变剂本身的亲和力很低,至少对这类基因型的亲和力很低,但这一因素仍会影响某些类型突变的频率。即使这种诱变剂的浓度极高,也不会导致突变数量的显著减少;不过,它们在可能的变化范围内达到了最大值。还需要进一步研究光合作用活性的变化和相关的持久性。
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引用次数: 0
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