Pub Date : 2023-09-20DOI: 10.21608/ejbo.2023.212452.2340
Tarek E. Abdelbaset, Hanan F. Elkammar, Sawsan M. Saeid, Ahmed E. Al Shorbagy, Khaled I. Saker
{"title":"Application of Silver Nanoparticles for Production of Sesamum Plants Free of Phytoplasma Disease in Tissue Culture","authors":"Tarek E. Abdelbaset, Hanan F. Elkammar, Sawsan M. Saeid, Ahmed E. Al Shorbagy, Khaled I. Saker","doi":"10.21608/ejbo.2023.212452.2340","DOIUrl":"https://doi.org/10.21608/ejbo.2023.212452.2340","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136377718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-20DOI: 10.21608/ejbo.2023.208980.2321
Hager Abd ElRahman, Mohamed A. Nasr-Eldin, Sabah A. Abo-Elmaaty, Mohamed A. Abdelwahed, Mahmoud ElHefnawi, Asmaa M. ElFiky, Elham R.S. Soliman
{"title":"Partial Genome Detection, Characterization of TYLCV (MZ546492) Infecting Tomato Plants and siRNA Sequences Detection for Alternative Control Strategy","authors":"Hager Abd ElRahman, Mohamed A. Nasr-Eldin, Sabah A. Abo-Elmaaty, Mohamed A. Abdelwahed, Mahmoud ElHefnawi, Asmaa M. ElFiky, Elham R.S. Soliman","doi":"10.21608/ejbo.2023.208980.2321","DOIUrl":"https://doi.org/10.21608/ejbo.2023.208980.2321","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136377716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.21608/ejbo.2023.175848.2197
Mohamed A. Ahmed, Mahmoud A.M. Saleh, Mahmoud M. Mansour
{"title":"The Prospective Impact of Some Edible Herbal Extracts on Cancer Cell Viability, Biochemical, and Cellular Immune Mediators In vitro","authors":"Mohamed A. Ahmed, Mahmoud A.M. Saleh, Mahmoud M. Mansour","doi":"10.21608/ejbo.2023.175848.2197","DOIUrl":"https://doi.org/10.21608/ejbo.2023.175848.2197","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135109096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.21608/ejbo.2023.211744.2343
Hamdy A. Elkhateeb, Mona I. Mabrouk, Amal S. Othman, M. K. Khaled
against MDR S. typhi and more safe than using meropenem drug.
{"title":"Antibacterial Efficacy of Macro and Nano Achillea millefolium L. against Multidrug-resistant Salmonella typhi with Genotyping Analysis","authors":"Hamdy A. Elkhateeb, Mona I. Mabrouk, Amal S. Othman, M. K. Khaled","doi":"10.21608/ejbo.2023.211744.2343","DOIUrl":"https://doi.org/10.21608/ejbo.2023.211744.2343","url":null,"abstract":"against MDR S. typhi and more safe than using meropenem drug.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"226 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135209666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.21608/ejbo.2023.214502.2359
Hebatallah Aly, Fatma A. Gharib, Eman Z. Ahmed
R OSEMARY ( Rosmarinus officinalis ) is a valuable aromatic herb native to the Mediterranean region. This study investigates the effects of foliar application of riboflavin on rosemary plants during two seasons in 2021. The first cutting season was in summer (August), and the second was in winter (December). Foliar application of riboflavin at different concentrations (25, 50, and 100mg L -l ) increased all growth attributes at two cuts by increasing nutrients, photosynthetic pigments, and photosynthetic activity. Riboflavin at 50mg L –l gave the highest mean values of all the measured growth attributes at both the first and second cuts. It also increased total photosynthetic pigments by 38%. Moreover, nutritional value, essential oil production, and overall antioxidant properties were positively enhanced in riboflavin-treated plants. Additionally, it showed the highest values of both element content and uptake. It increased oil yield (L fed –l ) by 116% and 160% at the 1st and 2nd cuts, respectively, with the highest antioxidant potential (IC 50 = 9.93µL mL –l ) at the second cut. Furthermore, riboflavin at 50 mg L –l enhanced the activities of antioxidant enzymes catalase and peroxidase. In contrast, the activities of oxidative enzymes including ascorbic acid oxidase, indole-3-acetic acid oxidase, polyphenol oxidase, and ribonuclease decreased. Furthermore, plants treated with 50mg L –l riboflavin increased the RNA and DNA content. These findings confirm the efficiency of the use of riboflavin at 50mg L –l to improve the growth of rosemary plants with high nutritional content and enhance the antioxidant activity of their essential oil.
{"title":"Response of Rosemary (Rosmarinus officinalis) Plants to Riboflavin Foliar Application: Improvement in Growth, Nutrient Content, Antioxidant Enzyme System, Essential Oil Production, and Its Antioxidant Potential","authors":"Hebatallah Aly, Fatma A. Gharib, Eman Z. Ahmed","doi":"10.21608/ejbo.2023.214502.2359","DOIUrl":"https://doi.org/10.21608/ejbo.2023.214502.2359","url":null,"abstract":"R OSEMARY ( Rosmarinus officinalis ) is a valuable aromatic herb native to the Mediterranean region. This study investigates the effects of foliar application of riboflavin on rosemary plants during two seasons in 2021. The first cutting season was in summer (August), and the second was in winter (December). Foliar application of riboflavin at different concentrations (25, 50, and 100mg L -l ) increased all growth attributes at two cuts by increasing nutrients, photosynthetic pigments, and photosynthetic activity. Riboflavin at 50mg L –l gave the highest mean values of all the measured growth attributes at both the first and second cuts. It also increased total photosynthetic pigments by 38%. Moreover, nutritional value, essential oil production, and overall antioxidant properties were positively enhanced in riboflavin-treated plants. Additionally, it showed the highest values of both element content and uptake. It increased oil yield (L fed –l ) by 116% and 160% at the 1st and 2nd cuts, respectively, with the highest antioxidant potential (IC 50 = 9.93µL mL –l ) at the second cut. Furthermore, riboflavin at 50 mg L –l enhanced the activities of antioxidant enzymes catalase and peroxidase. In contrast, the activities of oxidative enzymes including ascorbic acid oxidase, indole-3-acetic acid oxidase, polyphenol oxidase, and ribonuclease decreased. Furthermore, plants treated with 50mg L –l riboflavin increased the RNA and DNA content. These findings confirm the efficiency of the use of riboflavin at 50mg L –l to improve the growth of rosemary plants with high nutritional content and enhance the antioxidant activity of their essential oil.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.21608/ejbo.2023.218237.2383
Hanaa E.A. Amer
{"title":"Green Synthesized ZnO Nanoparticles Improve the Growth and Phytohormones Biosyn-thesis and Modulate the Expression of Resistance Genes in Phaseolus vulgaris","authors":"Hanaa E.A. Amer","doi":"10.21608/ejbo.2023.218237.2383","DOIUrl":"https://doi.org/10.21608/ejbo.2023.218237.2383","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.21608/ejbo.2023.225773.2436
Haidy Nasser, Mohamed S. Youssef, Aziza S. El -Kholy, Soliman A. Haroun
D ROUGHT is a major agronomic problem requiring immediate efforts to solve, to detect its effects on crop productivity. Tissue culture and mutagenic potentiality of EMS (ethyl methane sulfonate) were used to develop rice-promising drought-tolerant lines. Mature embryos of two genotypes (Sakha 101 and Giza 177) of rice were used for developing callus. Three callus induction strategies were investigated by applying EMS as a mutagen to evaluate and determine the best procedure for selection of drought-tolerant rice cell lines. For screening of calli response to PEG (polyethylene glycol), two-months-old well-proliferated treated calli of three protocols were sub-cultured for two weeks with 5% and 7% PEG-6000. Molecular response to PEG was evaluated by detecting gene expression of rice dehydrin, phytoglobin 1, 2, and 5 genes via qRT-PCR (quantitative real time-polymerase chain reaction). The relative gene expression of all genes was significantly increased in the PEG-treated calli compared with the control in both genotypes and Giza 177 showed higher expression levels than Sakha 101. Genetic variations were assessed among three putative mutants arising in vitro , their mother plants, and a drought-tolerant genotype (IET 1444) using ISSR fingerprinting. Similarity coefficients reflected the genetic relationship between rice regenerants and their mother plants. Cluster analysis showed regenerated Sakha 101 mutant line and IET 1444 were grouped together at a dissimilarity distance of 1.00. In vitro screening of EMS mutants with the creation of chemical drought using PEG-6000 to assess tolerance could be a good track to developing drought-tolerant rice lines.
{"title":"EMS-mutagenesis, In vitro Selection for Drought (PEG) Tolerance and Molecular Characterization of Mutants in Rice (Oryza sativa L.) Employing qRT-PCR and ISSR Markers","authors":"Haidy Nasser, Mohamed S. Youssef, Aziza S. El -Kholy, Soliman A. Haroun","doi":"10.21608/ejbo.2023.225773.2436","DOIUrl":"https://doi.org/10.21608/ejbo.2023.225773.2436","url":null,"abstract":"D ROUGHT is a major agronomic problem requiring immediate efforts to solve, to detect its effects on crop productivity. Tissue culture and mutagenic potentiality of EMS (ethyl methane sulfonate) were used to develop rice-promising drought-tolerant lines. Mature embryos of two genotypes (Sakha 101 and Giza 177) of rice were used for developing callus. Three callus induction strategies were investigated by applying EMS as a mutagen to evaluate and determine the best procedure for selection of drought-tolerant rice cell lines. For screening of calli response to PEG (polyethylene glycol), two-months-old well-proliferated treated calli of three protocols were sub-cultured for two weeks with 5% and 7% PEG-6000. Molecular response to PEG was evaluated by detecting gene expression of rice dehydrin, phytoglobin 1, 2, and 5 genes via qRT-PCR (quantitative real time-polymerase chain reaction). The relative gene expression of all genes was significantly increased in the PEG-treated calli compared with the control in both genotypes and Giza 177 showed higher expression levels than Sakha 101. Genetic variations were assessed among three putative mutants arising in vitro , their mother plants, and a drought-tolerant genotype (IET 1444) using ISSR fingerprinting. Similarity coefficients reflected the genetic relationship between rice regenerants and their mother plants. Cluster analysis showed regenerated Sakha 101 mutant line and IET 1444 were grouped together at a dissimilarity distance of 1.00. In vitro screening of EMS mutants with the creation of chemical drought using PEG-6000 to assess tolerance could be a good track to developing drought-tolerant rice lines.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.21608/ejbo.2023.212304.2338
Noha M. Kamel, Fatma F. Abdel-Motaal, Soad A. El-Zayat, Abou El-Hamd H. Mohamed, Shinji Ohta, Taha A. Hussien
{"title":"A new Secondary Metabolite with Antimicrobial, Antioxidant, and Cytotoxic Activities from an Endophytic Fungus, Gymnoascus thermotolerans","authors":"Noha M. Kamel, Fatma F. Abdel-Motaal, Soad A. El-Zayat, Abou El-Hamd H. Mohamed, Shinji Ohta, Taha A. Hussien","doi":"10.21608/ejbo.2023.212304.2338","DOIUrl":"https://doi.org/10.21608/ejbo.2023.212304.2338","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.21608/ejbo.2023.144429.2017
Marwa H. Azel, Sherif M. Sherif, Tarek A.A. Moussa, Mohamed A. El-Dessouky
{"title":"Isolation, Identification, and Characterization of Extracellular Siderophore Triacetylfusarinine C (TafC) Produced by Aspergillus fumigatus","authors":"Marwa H. Azel, Sherif M. Sherif, Tarek A.A. Moussa, Mohamed A. El-Dessouky","doi":"10.21608/ejbo.2023.144429.2017","DOIUrl":"https://doi.org/10.21608/ejbo.2023.144429.2017","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135248741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.21608/ejbo.2023.212700.2341
W. M. Adly, H. Abdelkader, Mahasen A. Mohamed, Mohammad E. EL-Denary, E. Sayed, A. Fouad
T HIS STUDY aimed to establish functional SSR markers corresponding to variations in starch accumulation in potato somaclones. In addition to the original cultivar Lady Rosetta, fourteen callus-sourced somaclones were investigated for their starch and sugar contents as well as dry matter content. Of the selected somaclones, one represented clones with the same starch content of the original cultivar and another represented those of lower starch content. The remaining somaclones have higher starch content, compared with original cultivar. The combined results of two growing seasons unveiled significant positive correlation between dry matter content and starch content which was absent when replacing starch with soluble sugars. Insignificant correlations were recorded between sugar content and starch content. Based on the transferability of SSR markers between species, six SSR primers characterizing variation in starch accumulation in sweet potatoes were exploited to assess genetic diversity among the selected potato clones. The analysis generated distinct and reproducible banding patterns with 68 bands, of which 62 were polymorphic. The utilized primers reflected high resolving power appeared in PIC of 0.457 in average and amplification of 44 unique bands, distributed in all studied clones except one of those containing higher starch content, compared with the original cultivar. This fingerprint is a prerequisite for recruiting clones with distinguished starch accumulation potential, addressed in the present study, in breeding programs targeting the improvement of starch accumulation in potato tubers. On the other hand, the utilized primers failed to establish phylogenetic relationship corresponding to starch content of the addressed clones. This may be attributed to utilization of few primers that did not cover a considerable proportion of the addressed genome.
{"title":"Development of SSR Markers to Characterize Potato (Solanum tuberosum L.) Soma clones with Improved Starch Accumulation","authors":"W. M. Adly, H. Abdelkader, Mahasen A. Mohamed, Mohammad E. EL-Denary, E. Sayed, A. Fouad","doi":"10.21608/ejbo.2023.212700.2341","DOIUrl":"https://doi.org/10.21608/ejbo.2023.212700.2341","url":null,"abstract":"T HIS STUDY aimed to establish functional SSR markers corresponding to variations in starch accumulation in potato somaclones. In addition to the original cultivar Lady Rosetta, fourteen callus-sourced somaclones were investigated for their starch and sugar contents as well as dry matter content. Of the selected somaclones, one represented clones with the same starch content of the original cultivar and another represented those of lower starch content. The remaining somaclones have higher starch content, compared with original cultivar. The combined results of two growing seasons unveiled significant positive correlation between dry matter content and starch content which was absent when replacing starch with soluble sugars. Insignificant correlations were recorded between sugar content and starch content. Based on the transferability of SSR markers between species, six SSR primers characterizing variation in starch accumulation in sweet potatoes were exploited to assess genetic diversity among the selected potato clones. The analysis generated distinct and reproducible banding patterns with 68 bands, of which 62 were polymorphic. The utilized primers reflected high resolving power appeared in PIC of 0.457 in average and amplification of 44 unique bands, distributed in all studied clones except one of those containing higher starch content, compared with the original cultivar. This fingerprint is a prerequisite for recruiting clones with distinguished starch accumulation potential, addressed in the present study, in breeding programs targeting the improvement of starch accumulation in potato tubers. On the other hand, the utilized primers failed to establish phylogenetic relationship corresponding to starch content of the addressed clones. This may be attributed to utilization of few primers that did not cover a considerable proportion of the addressed genome.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42043498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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