Pub Date : 2023-03-21DOI: 10.21608/ejbo.2023.155777.2097
Salma M. Awad, Tahani A. Hathout, Samia M. El khallal, K. Farroh
T HE AIM of this study was to determine how cerium dioxide nanoparticles (CeO 2 NPs) affect the growth and physiology of Brassica napus plants. CeO 2 NPs were biosynthesized using Aloe Vera extract and characterized using a UV-visible spectrophotometer, X-ray diffraction, Fourier transform infrared spectroscopy, Zeta potential, and particle size (DLS) as well as transmission electron microscopy. Following foliar treatment with 250, 500, and 1000ppm of CeO 2 NPs, the growth, and metabolism of Brassica napus were evaluated. Exposure to 500ppm CeO 2 NPs significantly enhanced morphological characteristics, antioxidant enzymes, phenol content, tocopherol, and chlorophyll content as well as carotenoid levels in B. napus . Assessment of oxidative stress in B. napus leaves treated with 500 CeO 2 NPs showed high levels of super oxide anion radical (o 2 ·¯) and hydrogen peroxide and low content of MDA, as well as inhibition in the activity of Lipoxygenase enzyme. Histochemical staining of B. napus leaves using nitro blue tetrazolium confirms the role of spraying of 500ppm of CeO 2 NPs in the accumulation of oxidative molecules in leaves. Gene expression assay for SOD and MT highlighted the role of CeO 2 NPs in the regulation of stress-related genes in tested plants. The changes in ultrastructure of Brassica napus leaves treated or untreated with 500ppm of CeO 2 NPs for two weeks were examined using a transmission electron microscope.
{"title":"Pleotropic Roles of Biosynthesized Cerium Oxide Nanoparticles on Morphological, Physiological and Molecular Aspects on Brassica napus","authors":"Salma M. Awad, Tahani A. Hathout, Samia M. El khallal, K. Farroh","doi":"10.21608/ejbo.2023.155777.2097","DOIUrl":"https://doi.org/10.21608/ejbo.2023.155777.2097","url":null,"abstract":"T HE AIM of this study was to determine how cerium dioxide nanoparticles (CeO 2 NPs) affect the growth and physiology of Brassica napus plants. CeO 2 NPs were biosynthesized using Aloe Vera extract and characterized using a UV-visible spectrophotometer, X-ray diffraction, Fourier transform infrared spectroscopy, Zeta potential, and particle size (DLS) as well as transmission electron microscopy. Following foliar treatment with 250, 500, and 1000ppm of CeO 2 NPs, the growth, and metabolism of Brassica napus were evaluated. Exposure to 500ppm CeO 2 NPs significantly enhanced morphological characteristics, antioxidant enzymes, phenol content, tocopherol, and chlorophyll content as well as carotenoid levels in B. napus . Assessment of oxidative stress in B. napus leaves treated with 500 CeO 2 NPs showed high levels of super oxide anion radical (o 2 ·¯) and hydrogen peroxide and low content of MDA, as well as inhibition in the activity of Lipoxygenase enzyme. Histochemical staining of B. napus leaves using nitro blue tetrazolium confirms the role of spraying of 500ppm of CeO 2 NPs in the accumulation of oxidative molecules in leaves. Gene expression assay for SOD and MT highlighted the role of CeO 2 NPs in the regulation of stress-related genes in tested plants. The changes in ultrastructure of Brassica napus leaves treated or untreated with 500ppm of CeO 2 NPs for two weeks were examined using a transmission electron microscope.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41549719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.21608/ejbo.2023.170207.2176
E. Farhan, R. A. Chechan
T HE local strain Lentinula edodes (OM432157), which was discovered for the first time in the Iraqi environment, was cultivated in the laboratory using nine treatments based on sawdust as the main substrate. A comparative study was done on the nutritional value of the local strain L. edodes (OM432157) in the wild and the cultivated one. It was observed that the highest growth rate of the fungus strain under study was in T8 (1.8 cm) and the lowest growth rate in T5 1.1 (cm). Fungal tissue growth in treatments (T4, and T5) were required (29 days), while in treatment T8 it was required (26 days), and in other remaining treatments it was accomplished in 25 days. The time required for pin heads to form in T5 was faster (17 days), followed by T8 (18 days) and T9 (19 days). The water-soluble vitamins (C, B1, B2) and the fat-soluble vitamins (E, K, D, A) were analyzed by HPLC. It was found that the cultivated mushrooms were a good source of vitamin B2 (6.9mg/100g dw) while wild mushrooms were a source of vitamin B1 (33.3mg/100g dw). The local strain of the cultivated and wild shiitake mushrooms also contained all fat-soluble vitamins, and the highest content of vitamin A was recorded in the cultivated type (19.9mg/100g dw) and the wild (103mg/100g.dw). These results are the first in Iraq, through which we found that the local strain L. edodes (OM432157), has high nutritional value and can be considered ideal supplements for many low-vitamin
{"title":"Effect of Some Agricultural Substrates on Production Efficiency of Lentinula edodes (OM432157) and Evaluation of its Vitamins Content","authors":"E. Farhan, R. A. Chechan","doi":"10.21608/ejbo.2023.170207.2176","DOIUrl":"https://doi.org/10.21608/ejbo.2023.170207.2176","url":null,"abstract":"T HE local strain Lentinula edodes (OM432157), which was discovered for the first time in the Iraqi environment, was cultivated in the laboratory using nine treatments based on sawdust as the main substrate. A comparative study was done on the nutritional value of the local strain L. edodes (OM432157) in the wild and the cultivated one. It was observed that the highest growth rate of the fungus strain under study was in T8 (1.8 cm) and the lowest growth rate in T5 1.1 (cm). Fungal tissue growth in treatments (T4, and T5) were required (29 days), while in treatment T8 it was required (26 days), and in other remaining treatments it was accomplished in 25 days. The time required for pin heads to form in T5 was faster (17 days), followed by T8 (18 days) and T9 (19 days). The water-soluble vitamins (C, B1, B2) and the fat-soluble vitamins (E, K, D, A) were analyzed by HPLC. It was found that the cultivated mushrooms were a good source of vitamin B2 (6.9mg/100g dw) while wild mushrooms were a source of vitamin B1 (33.3mg/100g dw). The local strain of the cultivated and wild shiitake mushrooms also contained all fat-soluble vitamins, and the highest content of vitamin A was recorded in the cultivated type (19.9mg/100g dw) and the wild (103mg/100g.dw). These results are the first in Iraq, through which we found that the local strain L. edodes (OM432157), has high nutritional value and can be considered ideal supplements for many low-vitamin","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46715712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.21608/ejbo.2023.185654.2224
W. Taia, Ahmed Hassan, Ghada E. El-Badan
L EAF macro-and micro-characters of seven genera represented by twenty species belonging to tribe Malveae, family Malvaceae were examined, measured and investigated in this study . The obtained results indicated that the petiole length, leaf blade shape, base and apex varied between the studied taxa. Meanwhile the leaf margin, number and depth of teeth/ cm in the leaf margin, type of venation and number of lateral veins can be of diagnostic value within the species. Epidermal cell characters are mostly invariable, while the type and density of hairs and presence of cavitated hairs beside the type of stomata and shape of guard cells gave valuable characters within the taxa. The results have been subjected to clustering analyses using PAST program and the resulted dendrogram shows great similarity between the taxa with the separation of both Malva aegyptia and Althaea ludwigii each in a separate clade. Diagnostic key has been instructed and the phylogenetic thinking has been postulated.
{"title":"Leaf Variations within Representative Genera of Tribe Malveae and their Significance in the Taxa Phylogeny","authors":"W. Taia, Ahmed Hassan, Ghada E. El-Badan","doi":"10.21608/ejbo.2023.185654.2224","DOIUrl":"https://doi.org/10.21608/ejbo.2023.185654.2224","url":null,"abstract":"L EAF macro-and micro-characters of seven genera represented by twenty species belonging to tribe Malveae, family Malvaceae were examined, measured and investigated in this study . The obtained results indicated that the petiole length, leaf blade shape, base and apex varied between the studied taxa. Meanwhile the leaf margin, number and depth of teeth/ cm in the leaf margin, type of venation and number of lateral veins can be of diagnostic value within the species. Epidermal cell characters are mostly invariable, while the type and density of hairs and presence of cavitated hairs beside the type of stomata and shape of guard cells gave valuable characters within the taxa. The results have been subjected to clustering analyses using PAST program and the resulted dendrogram shows great similarity between the taxa with the separation of both Malva aegyptia and Althaea ludwigii each in a separate clade. Diagnostic key has been instructed and the phylogenetic thinking has been postulated.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45747395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-13DOI: 10.21608/ejbo.2023.158720.2118
Mohamed E Abdel-Alim, M. Serag, H. Moussa, Mohamed A. Elgendy, M. Mohesien, Nesrein S. Salim
.
{"title":"Phytochemical Screening and Antioxidant Potential of Lotus corniculatusand Amaranthus viridis","authors":"Mohamed E Abdel-Alim, M. Serag, H. Moussa, Mohamed A. Elgendy, M. Mohesien, Nesrein S. Salim","doi":"10.21608/ejbo.2023.158720.2118","DOIUrl":"https://doi.org/10.21608/ejbo.2023.158720.2118","url":null,"abstract":".","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42903018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-07DOI: 10.21608/ejbo.2023.160063.2130
K. Shaltout, D. Ahmed, Y. Al-Sodany, S. Haroun, Mohmed M. El-Khalafy
{"title":"Cultural Importance Indices of the Endemic Plants in Egypt","authors":"K. Shaltout, D. Ahmed, Y. Al-Sodany, S. Haroun, Mohmed M. El-Khalafy","doi":"10.21608/ejbo.2023.160063.2130","DOIUrl":"https://doi.org/10.21608/ejbo.2023.160063.2130","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45689670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-07DOI: 10.21608/ejbo.2023.162736.2137
Zeinab M. Saleh, A. A. Abdel Azeiz, Ahmed B. M. Mehany, Z. A. El-Swaify
{"title":"Anticancer and Antimicrobial Activity of Jatropha’s Leaves Extracts","authors":"Zeinab M. Saleh, A. A. Abdel Azeiz, Ahmed B. M. Mehany, Z. A. El-Swaify","doi":"10.21608/ejbo.2023.162736.2137","DOIUrl":"https://doi.org/10.21608/ejbo.2023.162736.2137","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49008976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-07DOI: 10.21608/ejbo.2023.173612.2198
Enas G. Budran, Manal A. Abdelhamid, N. Hassan, M. N. Nemat Alla
{"title":"Ameliorative Effect of Ascorbate on Growth and Oil Fatty Acid Composition of Soybean under Salinity","authors":"Enas G. Budran, Manal A. Abdelhamid, N. Hassan, M. N. Nemat Alla","doi":"10.21608/ejbo.2023.173612.2198","DOIUrl":"https://doi.org/10.21608/ejbo.2023.173612.2198","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44190867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-06DOI: 10.21608/ejbo.2023.168006.2165
Mohamed A. Taha, H. Moussa, E. Dessoky
.
.
{"title":"The Influence of Spirulina platensis on Physiological Characterization and Mitigation of DNA Damage in Salt-stressed Phaseolus vulgaris L. Plants","authors":"Mohamed A. Taha, H. Moussa, E. Dessoky","doi":"10.21608/ejbo.2023.168006.2165","DOIUrl":"https://doi.org/10.21608/ejbo.2023.168006.2165","url":null,"abstract":".","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47274272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-06DOI: 10.21608/ejbo.2023.164643.2146
Kamartaha I. Saiyed, Kavi K. Oza, V. Raole
.
{"title":"Batrisu Vasanu: A Folk Polyherbal Formulation of Gujarat from the Perspective of Unani medicinal System","authors":"Kamartaha I. Saiyed, Kavi K. Oza, V. Raole","doi":"10.21608/ejbo.2023.164643.2146","DOIUrl":"https://doi.org/10.21608/ejbo.2023.164643.2146","url":null,"abstract":".","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49115364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T HYMUS and Salvia are among the most popular plants both in traditional medicine and in the culinary arts. This study has the aims to detect the chemical composition of the extracts and the powder of Thymus zygis L. (T) and Salvia officinalis L. (S) collected from the Fez-Meknes region. Two extracts were prepared: aqueous and essential oil. Phytochemical tests were performed to qualitatively evaluate the presence or absence of phytoconstituents using standard methods. The essential oils were analyzed by gas chromatography-mass spectrometry (GC/MS). Two powder analyses were performed: Fourier transform infrared spectroscopy (FTIR) analysis and elemental analysis. The drying of both plants took a similar amount of time with a noticeable loss in weight for Salvia . The phytochemical screening revealed the abundant presence of terpenoids, catechic tannins, steroids and sterols in the two plants. GC/MS analysis showed richness in carvacrol for Thymus zygis L. and in thujone for Salvia officinalis L. The analysis by FTIR showed characteristic peak readings of various functional groups in the powders, citing proteins, aliphatic compounds, carbonyl compounds and aromatic rings. In the elemental analysis, there is a high carbon content for Thymus and Salvia (T: 66.70%, S: 53.34%), followed by oxygen (T: 36.45%, S: 37.88%) and hydrogen (T: 6.08%, S: 5.61%). Altogether, this study highlights the richness of these two species in chemical compounds that can be used in the pharmaceutical industry.
{"title":"Phytochemical Investigation of Thymus zygis L. and Salvia officinalis L. Collected from Fez-Meknes Region, Morocco","authors":"Asmae Benabderrahmane, Majid Atmani, Abdellatif Boutagayout, Wijdane Rhioui, Saadia Belmalha","doi":"10.21608/ejbo.2023.181580.2215","DOIUrl":"https://doi.org/10.21608/ejbo.2023.181580.2215","url":null,"abstract":"T HYMUS and Salvia are among the most popular plants both in traditional medicine and in the culinary arts. This study has the aims to detect the chemical composition of the extracts and the powder of Thymus zygis L. (T) and Salvia officinalis L. (S) collected from the Fez-Meknes region. Two extracts were prepared: aqueous and essential oil. Phytochemical tests were performed to qualitatively evaluate the presence or absence of phytoconstituents using standard methods. The essential oils were analyzed by gas chromatography-mass spectrometry (GC/MS). Two powder analyses were performed: Fourier transform infrared spectroscopy (FTIR) analysis and elemental analysis. The drying of both plants took a similar amount of time with a noticeable loss in weight for Salvia . The phytochemical screening revealed the abundant presence of terpenoids, catechic tannins, steroids and sterols in the two plants. GC/MS analysis showed richness in carvacrol for Thymus zygis L. and in thujone for Salvia officinalis L. The analysis by FTIR showed characteristic peak readings of various functional groups in the powders, citing proteins, aliphatic compounds, carbonyl compounds and aromatic rings. In the elemental analysis, there is a high carbon content for Thymus and Salvia (T: 66.70%, S: 53.34%), followed by oxygen (T: 36.45%, S: 37.88%) and hydrogen (T: 6.08%, S: 5.61%). Altogether, this study highlights the richness of these two species in chemical compounds that can be used in the pharmaceutical industry.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47590272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}