Pub Date : 2023-09-13DOI: 10.21608/ejbo.2023.218237.2383
Hanaa E.A. Amer
{"title":"Green Synthesized ZnO Nanoparticles Improve the Growth and Phytohormones Biosyn-thesis and Modulate the Expression of Resistance Genes in Phaseolus vulgaris","authors":"Hanaa E.A. Amer","doi":"10.21608/ejbo.2023.218237.2383","DOIUrl":"https://doi.org/10.21608/ejbo.2023.218237.2383","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.21608/ejbo.2023.225773.2436
Haidy Nasser, Mohamed S. Youssef, Aziza S. El -Kholy, Soliman A. Haroun
D ROUGHT is a major agronomic problem requiring immediate efforts to solve, to detect its effects on crop productivity. Tissue culture and mutagenic potentiality of EMS (ethyl methane sulfonate) were used to develop rice-promising drought-tolerant lines. Mature embryos of two genotypes (Sakha 101 and Giza 177) of rice were used for developing callus. Three callus induction strategies were investigated by applying EMS as a mutagen to evaluate and determine the best procedure for selection of drought-tolerant rice cell lines. For screening of calli response to PEG (polyethylene glycol), two-months-old well-proliferated treated calli of three protocols were sub-cultured for two weeks with 5% and 7% PEG-6000. Molecular response to PEG was evaluated by detecting gene expression of rice dehydrin, phytoglobin 1, 2, and 5 genes via qRT-PCR (quantitative real time-polymerase chain reaction). The relative gene expression of all genes was significantly increased in the PEG-treated calli compared with the control in both genotypes and Giza 177 showed higher expression levels than Sakha 101. Genetic variations were assessed among three putative mutants arising in vitro , their mother plants, and a drought-tolerant genotype (IET 1444) using ISSR fingerprinting. Similarity coefficients reflected the genetic relationship between rice regenerants and their mother plants. Cluster analysis showed regenerated Sakha 101 mutant line and IET 1444 were grouped together at a dissimilarity distance of 1.00. In vitro screening of EMS mutants with the creation of chemical drought using PEG-6000 to assess tolerance could be a good track to developing drought-tolerant rice lines.
{"title":"EMS-mutagenesis, In vitro Selection for Drought (PEG) Tolerance and Molecular Characterization of Mutants in Rice (Oryza sativa L.) Employing qRT-PCR and ISSR Markers","authors":"Haidy Nasser, Mohamed S. Youssef, Aziza S. El -Kholy, Soliman A. Haroun","doi":"10.21608/ejbo.2023.225773.2436","DOIUrl":"https://doi.org/10.21608/ejbo.2023.225773.2436","url":null,"abstract":"D ROUGHT is a major agronomic problem requiring immediate efforts to solve, to detect its effects on crop productivity. Tissue culture and mutagenic potentiality of EMS (ethyl methane sulfonate) were used to develop rice-promising drought-tolerant lines. Mature embryos of two genotypes (Sakha 101 and Giza 177) of rice were used for developing callus. Three callus induction strategies were investigated by applying EMS as a mutagen to evaluate and determine the best procedure for selection of drought-tolerant rice cell lines. For screening of calli response to PEG (polyethylene glycol), two-months-old well-proliferated treated calli of three protocols were sub-cultured for two weeks with 5% and 7% PEG-6000. Molecular response to PEG was evaluated by detecting gene expression of rice dehydrin, phytoglobin 1, 2, and 5 genes via qRT-PCR (quantitative real time-polymerase chain reaction). The relative gene expression of all genes was significantly increased in the PEG-treated calli compared with the control in both genotypes and Giza 177 showed higher expression levels than Sakha 101. Genetic variations were assessed among three putative mutants arising in vitro , their mother plants, and a drought-tolerant genotype (IET 1444) using ISSR fingerprinting. Similarity coefficients reflected the genetic relationship between rice regenerants and their mother plants. Cluster analysis showed regenerated Sakha 101 mutant line and IET 1444 were grouped together at a dissimilarity distance of 1.00. In vitro screening of EMS mutants with the creation of chemical drought using PEG-6000 to assess tolerance could be a good track to developing drought-tolerant rice lines.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.21608/ejbo.2023.212304.2338
Noha M. Kamel, Fatma F. Abdel-Motaal, Soad A. El-Zayat, Abou El-Hamd H. Mohamed, Shinji Ohta, Taha A. Hussien
{"title":"A new Secondary Metabolite with Antimicrobial, Antioxidant, and Cytotoxic Activities from an Endophytic Fungus, Gymnoascus thermotolerans","authors":"Noha M. Kamel, Fatma F. Abdel-Motaal, Soad A. El-Zayat, Abou El-Hamd H. Mohamed, Shinji Ohta, Taha A. Hussien","doi":"10.21608/ejbo.2023.212304.2338","DOIUrl":"https://doi.org/10.21608/ejbo.2023.212304.2338","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.21608/ejbo.2023.144429.2017
Marwa H. Azel, Sherif M. Sherif, Tarek A.A. Moussa, Mohamed A. El-Dessouky
{"title":"Isolation, Identification, and Characterization of Extracellular Siderophore Triacetylfusarinine C (TafC) Produced by Aspergillus fumigatus","authors":"Marwa H. Azel, Sherif M. Sherif, Tarek A.A. Moussa, Mohamed A. El-Dessouky","doi":"10.21608/ejbo.2023.144429.2017","DOIUrl":"https://doi.org/10.21608/ejbo.2023.144429.2017","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135248741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.21608/ejbo.2023.212700.2341
W. M. Adly, H. Abdelkader, Mahasen A. Mohamed, Mohammad E. EL-Denary, E. Sayed, A. Fouad
T HIS STUDY aimed to establish functional SSR markers corresponding to variations in starch accumulation in potato somaclones. In addition to the original cultivar Lady Rosetta, fourteen callus-sourced somaclones were investigated for their starch and sugar contents as well as dry matter content. Of the selected somaclones, one represented clones with the same starch content of the original cultivar and another represented those of lower starch content. The remaining somaclones have higher starch content, compared with original cultivar. The combined results of two growing seasons unveiled significant positive correlation between dry matter content and starch content which was absent when replacing starch with soluble sugars. Insignificant correlations were recorded between sugar content and starch content. Based on the transferability of SSR markers between species, six SSR primers characterizing variation in starch accumulation in sweet potatoes were exploited to assess genetic diversity among the selected potato clones. The analysis generated distinct and reproducible banding patterns with 68 bands, of which 62 were polymorphic. The utilized primers reflected high resolving power appeared in PIC of 0.457 in average and amplification of 44 unique bands, distributed in all studied clones except one of those containing higher starch content, compared with the original cultivar. This fingerprint is a prerequisite for recruiting clones with distinguished starch accumulation potential, addressed in the present study, in breeding programs targeting the improvement of starch accumulation in potato tubers. On the other hand, the utilized primers failed to establish phylogenetic relationship corresponding to starch content of the addressed clones. This may be attributed to utilization of few primers that did not cover a considerable proportion of the addressed genome.
{"title":"Development of SSR Markers to Characterize Potato (Solanum tuberosum L.) Soma clones with Improved Starch Accumulation","authors":"W. M. Adly, H. Abdelkader, Mahasen A. Mohamed, Mohammad E. EL-Denary, E. Sayed, A. Fouad","doi":"10.21608/ejbo.2023.212700.2341","DOIUrl":"https://doi.org/10.21608/ejbo.2023.212700.2341","url":null,"abstract":"T HIS STUDY aimed to establish functional SSR markers corresponding to variations in starch accumulation in potato somaclones. In addition to the original cultivar Lady Rosetta, fourteen callus-sourced somaclones were investigated for their starch and sugar contents as well as dry matter content. Of the selected somaclones, one represented clones with the same starch content of the original cultivar and another represented those of lower starch content. The remaining somaclones have higher starch content, compared with original cultivar. The combined results of two growing seasons unveiled significant positive correlation between dry matter content and starch content which was absent when replacing starch with soluble sugars. Insignificant correlations were recorded between sugar content and starch content. Based on the transferability of SSR markers between species, six SSR primers characterizing variation in starch accumulation in sweet potatoes were exploited to assess genetic diversity among the selected potato clones. The analysis generated distinct and reproducible banding patterns with 68 bands, of which 62 were polymorphic. The utilized primers reflected high resolving power appeared in PIC of 0.457 in average and amplification of 44 unique bands, distributed in all studied clones except one of those containing higher starch content, compared with the original cultivar. This fingerprint is a prerequisite for recruiting clones with distinguished starch accumulation potential, addressed in the present study, in breeding programs targeting the improvement of starch accumulation in potato tubers. On the other hand, the utilized primers failed to establish phylogenetic relationship corresponding to starch content of the addressed clones. This may be attributed to utilization of few primers that did not cover a considerable proportion of the addressed genome.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42043498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.21608/ejbo.2023.191189.2245
H. Zaki, N. El-Tayeh, Fayrouz Abo El Hagag, N. Youssef
{"title":"Correlation between Genetic- physiological Traits of Egyptian Lupin (Lupinus termis) Induced by Drought Stress","authors":"H. Zaki, N. El-Tayeh, Fayrouz Abo El Hagag, N. Youssef","doi":"10.21608/ejbo.2023.191189.2245","DOIUrl":"https://doi.org/10.21608/ejbo.2023.191189.2245","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48512713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.21608/ejbo.2023.220267.2393
Mofida E. M. Makhlof, M. El-sheekh, Majdah M. Y. Al-Tuwaijri, K. El-Tarabily, A. Elsayed
U LVA selenium nanoparticles (USeNPs) were synthesized and characterized, then examined for their wound healing capacity through in vitro assay showing promising healing (82.27%) with USeNPs concentration (500µg/mL). We also determined the antifungal potential via agar well diffusion technique, minimum inhibitory concentration (MIC), which was evaluated by a micro-dilution assay. The synergistic effect of the USeNPs individually or in combination with standard antifungal antibiotic (ketoconazole;100μg/mL) was studied against Cryptococcus neoformans RCMB 0049001 and Candida lipolytica RCMB 005007(1). Geotrichum candidum RCMB 041001 was also checked by agar well diffusion assay, followed by transmission electron microscopy to assess different changes in the most sensitive fungal yeast cells. The results indicated that USeNPs have a promising antifungal effect against C. neoformans RCMB 0049001 and C. lipolytica RCMB 005007(1) by achieving remarkable inhibition zones. In comparison, no effect was detected on G. candidum RCMB 041001. The MIC was found to be 78.1μg and 312.5μg for C. neoformans RCMB 0049001 and C. lipolytica RCMB 005007(1), respectively. The biosynthetic USeNPs have a strong antifungal potential and can elevate and strengthen the action of ketoconazole antibiotic towards different fungal pathogens, and this was obvious In the experiment of synergism carried out in the present study based on combining ketoconazole;100μg/mL with USeNPs, which resulted in a detached C. neoformas RCMB 0049001 cells with a severe dramatic change.
{"title":"In vitro Assessment of Ulva lactuca Mediated Selenium Nanoparticles (USeNPs) through Elevating the Action of Ketoconazole Antibiotic against Pathogenic Yeast Species and Wound Healing Capacity through Inhibition of Cyclooxygenase (Cox-1) Activity","authors":"Mofida E. M. Makhlof, M. El-sheekh, Majdah M. Y. Al-Tuwaijri, K. El-Tarabily, A. Elsayed","doi":"10.21608/ejbo.2023.220267.2393","DOIUrl":"https://doi.org/10.21608/ejbo.2023.220267.2393","url":null,"abstract":"U LVA selenium nanoparticles (USeNPs) were synthesized and characterized, then examined for their wound healing capacity through in vitro assay showing promising healing (82.27%) with USeNPs concentration (500µg/mL). We also determined the antifungal potential via agar well diffusion technique, minimum inhibitory concentration (MIC), which was evaluated by a micro-dilution assay. The synergistic effect of the USeNPs individually or in combination with standard antifungal antibiotic (ketoconazole;100μg/mL) was studied against Cryptococcus neoformans RCMB 0049001 and Candida lipolytica RCMB 005007(1). Geotrichum candidum RCMB 041001 was also checked by agar well diffusion assay, followed by transmission electron microscopy to assess different changes in the most sensitive fungal yeast cells. The results indicated that USeNPs have a promising antifungal effect against C. neoformans RCMB 0049001 and C. lipolytica RCMB 005007(1) by achieving remarkable inhibition zones. In comparison, no effect was detected on G. candidum RCMB 041001. The MIC was found to be 78.1μg and 312.5μg for C. neoformans RCMB 0049001 and C. lipolytica RCMB 005007(1), respectively. The biosynthetic USeNPs have a strong antifungal potential and can elevate and strengthen the action of ketoconazole antibiotic towards different fungal pathogens, and this was obvious In the experiment of synergism carried out in the present study based on combining ketoconazole;100μg/mL with USeNPs, which resulted in a detached C. neoformas RCMB 0049001 cells with a severe dramatic change.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43441809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-07DOI: 10.21608/ejbo.2023.210573.2330
Tamer Bouback, F. Al-Sarraj, I. Alotibi, R. Albiheyri, Majed Al-Zahrani, Mashail A. Alghamdi, Nada M. Nass, B. Sajer, A. Bamagoos, S. Azhari, R. Farsi
S TAPHYLOCOCCUS aureus can enter the bloodstream, leading to health complications such as sepsis, arthritis, endocarditis
{"title":"Convergent Replication and Mobilization Mechanism of Staphylococcus Pathogenicity Islands (SaPIbov5) by Interfering with Bacteriophage 12 Production Models","authors":"Tamer Bouback, F. Al-Sarraj, I. Alotibi, R. Albiheyri, Majed Al-Zahrani, Mashail A. Alghamdi, Nada M. Nass, B. Sajer, A. Bamagoos, S. Azhari, R. Farsi","doi":"10.21608/ejbo.2023.210573.2330","DOIUrl":"https://doi.org/10.21608/ejbo.2023.210573.2330","url":null,"abstract":"S TAPHYLOCOCCUS aureus can enter the bloodstream, leading to health complications such as sepsis, arthritis, endocarditis","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48961937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/ejbo.2023.189298.2235
S. Alharbi
C EROPEGIA lodarensis (≡ Huernia lodarensis) is a succulent plant with medicinal uses in Arabian folk medicine. It belongs to Ceropegia sect . Huernia, the most diverse and widespread section of the highly succulent plants informally known as stapeliads. Morphological identification of this species is difficult and often impossible without flowers. This study tested the performance of three DNA barcodes to identify C. lodarensis at the molecular level. These DNA barcodes are: the nuclear ribosomal internal transcribed spacer (ITS) region, plastid psb A– trn H intergenic region, and noncoding plastid trn L– trn F. The identification was done using BLASTn searches; and tree-based methods, namely maximum likelihood (ML), maximum parsimony (MP) and neighbour joining (NJ). Results showed that the candidate regions have a low species-level resolving ability . However, ITS has the highest identification success rate at the sect. Huernia level, suggesting its potential for plant identification systems of Huernia species at the section level. Additional work is needed to examine the utility of further DNA loci.
{"title":"Evaluating the Efficacy of Three DNA Barcodes in Identifying the Medicinal Species Ceropegia lodarensis (Lavranos) Bruyns (Apocynaceae)","authors":"S. Alharbi","doi":"10.21608/ejbo.2023.189298.2235","DOIUrl":"https://doi.org/10.21608/ejbo.2023.189298.2235","url":null,"abstract":"C EROPEGIA lodarensis (≡ Huernia lodarensis) is a succulent plant with medicinal uses in Arabian folk medicine. It belongs to Ceropegia sect . Huernia, the most diverse and widespread section of the highly succulent plants informally known as stapeliads. Morphological identification of this species is difficult and often impossible without flowers. This study tested the performance of three DNA barcodes to identify C. lodarensis at the molecular level. These DNA barcodes are: the nuclear ribosomal internal transcribed spacer (ITS) region, plastid psb A– trn H intergenic region, and noncoding plastid trn L– trn F. The identification was done using BLASTn searches; and tree-based methods, namely maximum likelihood (ML), maximum parsimony (MP) and neighbour joining (NJ). Results showed that the candidate regions have a low species-level resolving ability . However, ITS has the highest identification success rate at the sect. Huernia level, suggesting its potential for plant identification systems of Huernia species at the section level. Additional work is needed to examine the utility of further DNA loci.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48789367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21608/ejbo.2023.187059.2226
A. Shehata, W. Taia, M. A. Shiha, S.A.M. Eltelet, M. Salim
{"title":"Pollen Morphology on Some Crassulaceae DC.","authors":"A. Shehata, W. Taia, M. A. Shiha, S.A.M. Eltelet, M. Salim","doi":"10.21608/ejbo.2023.187059.2226","DOIUrl":"https://doi.org/10.21608/ejbo.2023.187059.2226","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48389048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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