Pub Date : 2023-05-07DOI: 10.21608/ejbo.2023.172401.2183
M. R. Zaki, Taha D. Farrag, A. Mohamedin, M. El-Bana, W. Saber
D ESPITE expensive treatment procedures designed to eliminate harmful germs from drinkable water supplies and waterborne illnesses, they still pose a threat to public health. The current investigation is concerned with the removal of Gram-positive and Gram-negative pathogenic bacteria from aqueous solutions. The abundant and inexpensive natural resource, pomegranate dry peel (PDP), and its activated carbon (PDPAC) have been used as bio-adsorbents. Phosphoric acid (30%) was used to chemically activate the carbon at 800 ºC. The optimum contact time was 60min for the dry peel and 40min for the activated carbon at a pH range of 7.0 to 7.5 and 30 to 35ºC processing temperature. The adsorption isotherm of different bacteria was determined for both adsorbents and analyzed by three common isotherm models. The equilibrium results have matched well to the Langmuir equation and the maximal monolayer adsorption capacity was 12500, 14286, 20000, 1000 CFU/g with PDP and 25000, 25010, 33343, 20000 CFU/g with PDPAC for E. coli, S. enterica, S. aureus , and E. faecalis, respectively. This research can be applied in the treatment stages of water treatment plants, sewage treatment plants, and household filters as a low-cost adsorbent material to remove pathogenic bacteria, antibiotic-resistant bacteria, and
{"title":"Removal of Pathogenic Bacteria from Water Using Pomegranate Peels","authors":"M. R. Zaki, Taha D. Farrag, A. Mohamedin, M. El-Bana, W. Saber","doi":"10.21608/ejbo.2023.172401.2183","DOIUrl":"https://doi.org/10.21608/ejbo.2023.172401.2183","url":null,"abstract":"D ESPITE expensive treatment procedures designed to eliminate harmful germs from drinkable water supplies and waterborne illnesses, they still pose a threat to public health. The current investigation is concerned with the removal of Gram-positive and Gram-negative pathogenic bacteria from aqueous solutions. The abundant and inexpensive natural resource, pomegranate dry peel (PDP), and its activated carbon (PDPAC) have been used as bio-adsorbents. Phosphoric acid (30%) was used to chemically activate the carbon at 800 ºC. The optimum contact time was 60min for the dry peel and 40min for the activated carbon at a pH range of 7.0 to 7.5 and 30 to 35ºC processing temperature. The adsorption isotherm of different bacteria was determined for both adsorbents and analyzed by three common isotherm models. The equilibrium results have matched well to the Langmuir equation and the maximal monolayer adsorption capacity was 12500, 14286, 20000, 1000 CFU/g with PDP and 25000, 25010, 33343, 20000 CFU/g with PDPAC for E. coli, S. enterica, S. aureus , and E. faecalis, respectively. This research can be applied in the treatment stages of water treatment plants, sewage treatment plants, and household filters as a low-cost adsorbent material to remove pathogenic bacteria, antibiotic-resistant bacteria, and","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47024931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-03DOI: 10.21608/ejbo.2023.199400.2277
S. Mohamed, Mohamed M. El-Mahdy, Alshaimaa M. Mabrouk, R. Salem, M. Shehata, I. El-Kholy, Heba H. Abouseadaa
{"title":"Molecular Characterization, Heterologous Expression and Antimicrobial Activity of Phaseolus vulgaris L. Defensin Peptide (Pv-Def) against various Human MDR Pathogens","authors":"S. Mohamed, Mohamed M. El-Mahdy, Alshaimaa M. Mabrouk, R. Salem, M. Shehata, I. El-Kholy, Heba H. Abouseadaa","doi":"10.21608/ejbo.2023.199400.2277","DOIUrl":"https://doi.org/10.21608/ejbo.2023.199400.2277","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48602237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-03DOI: 10.21608/ejbo.2023.189961.2247
Umme Qulsum, M. T. A. Azad, T. Tsukahara
{"title":"Identification of Zinc Finger (ZnF) Genes that Undergo Tissue-specific Alternative Splicing in Arabidopsis thaliana","authors":"Umme Qulsum, M. T. A. Azad, T. Tsukahara","doi":"10.21608/ejbo.2023.189961.2247","DOIUrl":"https://doi.org/10.21608/ejbo.2023.189961.2247","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44627446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-03DOI: 10.21608/ejbo.2023.174829.2193
N. E. Mustamu, Koko Tampubolon, Alridiwirsah Alridiwirsah, M. Basyuni
T HE use of local maize is still in high demand by farmers in Indonesia. The development of local maize needs to be discovered through a morphological approach. This study was to obtain seed characteristics and similarities of local maize from North Sumatra. Local maize accession seeds were taken by four districts/cities (Binjai, Serdang Bedagai, Deli Serdang, and Mandailing Natal) from North Sumatra, Indonesia from July to September 2022. A hybrid variety of maize was selected for comparison. Seed characteristics were measured in each accession with four replications. A descriptive-analytic method was selected in this study and data were analyzed using one-way ANOVA and followed by Tukey at P <0.05. Correlation analysis, similarity matrix, hierarchical cluster, and constellation plot were constructed in this study. The results showed that SB-1; DS-3; SB-4; and SB-2 accessions had higher seed characteristics than other accessions. The SB-5 accession had the nearest neighbors (0.743) and two accessions (BI-1; DS-2) were classified into one cluster with a hybrid variety. This finding indicates that several local maize accessions are nearest to the hybrid characteristics. A novelty of this study informed that local maize had an opportunity to be used in the fields.
{"title":"The Cluster of Local Maize Accessions Based on Seed Characteristics from North Sumatra","authors":"N. E. Mustamu, Koko Tampubolon, Alridiwirsah Alridiwirsah, M. Basyuni","doi":"10.21608/ejbo.2023.174829.2193","DOIUrl":"https://doi.org/10.21608/ejbo.2023.174829.2193","url":null,"abstract":"T HE use of local maize is still in high demand by farmers in Indonesia. The development of local maize needs to be discovered through a morphological approach. This study was to obtain seed characteristics and similarities of local maize from North Sumatra. Local maize accession seeds were taken by four districts/cities (Binjai, Serdang Bedagai, Deli Serdang, and Mandailing Natal) from North Sumatra, Indonesia from July to September 2022. A hybrid variety of maize was selected for comparison. Seed characteristics were measured in each accession with four replications. A descriptive-analytic method was selected in this study and data were analyzed using one-way ANOVA and followed by Tukey at P <0.05. Correlation analysis, similarity matrix, hierarchical cluster, and constellation plot were constructed in this study. The results showed that SB-1; DS-3; SB-4; and SB-2 accessions had higher seed characteristics than other accessions. The SB-5 accession had the nearest neighbors (0.743) and two accessions (BI-1; DS-2) were classified into one cluster with a hybrid variety. This finding indicates that several local maize accessions are nearest to the hybrid characteristics. A novelty of this study informed that local maize had an opportunity to be used in the fields.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44026882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-18DOI: 10.21608/ejbo.2023.191783.2246
S. Ouf, Fawkia El-Amriti, Mohammed Abu-Elghait, S. Desouky, Mahmoud S.M. Mohamed
.
.
{"title":"Role of Plant Growth Promoting Rhizobacteria in Healthy and Sustainable Agriculture","authors":"S. Ouf, Fawkia El-Amriti, Mohammed Abu-Elghait, S. Desouky, Mahmoud S.M. Mohamed","doi":"10.21608/ejbo.2023.191783.2246","DOIUrl":"https://doi.org/10.21608/ejbo.2023.191783.2246","url":null,"abstract":".","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48972484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-06DOI: 10.21608/ejbo.2023.189448.2236
Omnia S. Mousa, Noha M. Abd El Hameed, Adel ELMehalawy, S. S. Mohamed
F IBRINOLYTIC enzyme production from Scopulariopsis brevicaulis OS 3456 isolated from a local soil sample was studied. The enzyme was purified by ammonium sulfate precipitation and gel filtration chromatography using Sephadex G-100, increasing its specific activity to 370 U/mg with a yield of 1.5% and a purification fold of 3.4. The molecular weight of the purified enzyme was 61.5 kDa determined by SDS-PAGE analysis. The optimum temperature of the enzyme was 37 o C, and it was stable over a pH range of 5.0–9.0 with maximum stability at pH 7.0. The activity was increased in the presence of ß-mercaptoethanol, Mn 2+ , Ba 2+ , triton X-100, and xylene by 137.1, 51.6, 41.4, 37.5, and 23%, respectively. Furthermore, the enzyme activity was inhibited by Cd 2+ , Al 3+ , EDTA, PMSF, and acetone. The in vitro thrombolytic activity of the undiluted purified enzyme (370 U/mg) was found to be 100%. Meanwhile, in the cases of 185, 92.5, 46.25, 23.125, and 11.562 U/mg, the clot lysis percentage was 76.8, 67.4, 57.8, 39.5, and 28%, respectively. A carrageenan-induced tail thrombosis model was applied to test the in vivo thrombolytic activity of the enzyme. The result indicated no obvious thrombus in the tails of mice treated with the tested enzyme (370 U/mg). However, when the enzyme was diluted, its thrombolytic activity decreased gradually. All these results explore the promising thrombolytic activity of the extracted fibrinolytic enzyme. Hence, more purification steps and more experimental animal studies are required in the future for its use as a commercial drug.
{"title":"Novel Fibrinolytic Enzyme by Scopulariopsis brevicaulis OS 3456: Production, Characterization, In vitro, and In vivo Activity","authors":"Omnia S. Mousa, Noha M. Abd El Hameed, Adel ELMehalawy, S. S. Mohamed","doi":"10.21608/ejbo.2023.189448.2236","DOIUrl":"https://doi.org/10.21608/ejbo.2023.189448.2236","url":null,"abstract":"F IBRINOLYTIC enzyme production from Scopulariopsis brevicaulis OS 3456 isolated from a local soil sample was studied. The enzyme was purified by ammonium sulfate precipitation and gel filtration chromatography using Sephadex G-100, increasing its specific activity to 370 U/mg with a yield of 1.5% and a purification fold of 3.4. The molecular weight of the purified enzyme was 61.5 kDa determined by SDS-PAGE analysis. The optimum temperature of the enzyme was 37 o C, and it was stable over a pH range of 5.0–9.0 with maximum stability at pH 7.0. The activity was increased in the presence of ß-mercaptoethanol, Mn 2+ , Ba 2+ , triton X-100, and xylene by 137.1, 51.6, 41.4, 37.5, and 23%, respectively. Furthermore, the enzyme activity was inhibited by Cd 2+ , Al 3+ , EDTA, PMSF, and acetone. The in vitro thrombolytic activity of the undiluted purified enzyme (370 U/mg) was found to be 100%. Meanwhile, in the cases of 185, 92.5, 46.25, 23.125, and 11.562 U/mg, the clot lysis percentage was 76.8, 67.4, 57.8, 39.5, and 28%, respectively. A carrageenan-induced tail thrombosis model was applied to test the in vivo thrombolytic activity of the enzyme. The result indicated no obvious thrombus in the tails of mice treated with the tested enzyme (370 U/mg). However, when the enzyme was diluted, its thrombolytic activity decreased gradually. All these results explore the promising thrombolytic activity of the extracted fibrinolytic enzyme. Hence, more purification steps and more experimental animal studies are required in the future for its use as a commercial drug.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48843830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-02DOI: 10.21608/ejbo.2023.176281.2199
D. Ahmed, M. EL-KHALAFY, Fathi Almushghub, A. Sharaf El-Din, K. Shaltout
T HIS PAPER compares four indices; each index is designed to assess the cultural significance of plant species through the study of the goods provided by wild plants in the study area and the assessment of the Cultural Importance (CI) index, which reflects the cultural importance of wild plant usage. The results show that 94 species have a useful use (63.8% for medicinal use, 60.6% for beekeeping, 43.6% for fodder plants, 27.7% for human food, 8.5% as fuel and 2.1% in hand-made industries). The total CI of each category indicated that plants used in beekeeping are the most common among inhabitants (9.54), followed by medicinal use (9.46), and grazing (7.77), while hand-made was the least (0.27). Ziziphus lotus (total CI=1.22) had first rank in cultural using the four quantitative indices; CI, the relative frequency of citation (RFC), relative importance (RI) and cultural value (CV); with five number of uses (NU), 82 number of use reports (UR) and 41 frequency of citation (FC). There is a positive and significant relationship between the NU and FC (r = 0.876). At the same time, CI is significantly highly correlated with FC (r= 0.975), and CV was the most correlated with FC (r= 0.98). Despite the controversy surrounding the use of cultural significance indices, authors believe that indices based on interviews are the only way to fill passive knowledge about valuable plants. It is crucial to study the inhabitant’s cultural uses of wild plants, to reflect their economic importance and the importance to be conservation.
{"title":"Ethnobotanical Importance of Wild Plants in Wadi Kaam, Northwestern Libya","authors":"D. Ahmed, M. EL-KHALAFY, Fathi Almushghub, A. Sharaf El-Din, K. Shaltout","doi":"10.21608/ejbo.2023.176281.2199","DOIUrl":"https://doi.org/10.21608/ejbo.2023.176281.2199","url":null,"abstract":"T HIS PAPER compares four indices; each index is designed to assess the cultural significance of plant species through the study of the goods provided by wild plants in the study area and the assessment of the Cultural Importance (CI) index, which reflects the cultural importance of wild plant usage. The results show that 94 species have a useful use (63.8% for medicinal use, 60.6% for beekeeping, 43.6% for fodder plants, 27.7% for human food, 8.5% as fuel and 2.1% in hand-made industries). The total CI of each category indicated that plants used in beekeeping are the most common among inhabitants (9.54), followed by medicinal use (9.46), and grazing (7.77), while hand-made was the least (0.27). Ziziphus lotus (total CI=1.22) had first rank in cultural using the four quantitative indices; CI, the relative frequency of citation (RFC), relative importance (RI) and cultural value (CV); with five number of uses (NU), 82 number of use reports (UR) and 41 frequency of citation (FC). There is a positive and significant relationship between the NU and FC (r = 0.876). At the same time, CI is significantly highly correlated with FC (r= 0.975), and CV was the most correlated with FC (r= 0.98). Despite the controversy surrounding the use of cultural significance indices, authors believe that indices based on interviews are the only way to fill passive knowledge about valuable plants. It is crucial to study the inhabitant’s cultural uses of wild plants, to reflect their economic importance and the importance to be conservation.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44271642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-21DOI: 10.21608/ejbo.2023.155080.2078
Emad A. Shalaby, S. Shanab, R. Hafez, A. E. El-Ansary
{"title":"Metabolomics and Biological Activities of Residual Parts from some Egyptian Green Vegetables","authors":"Emad A. Shalaby, S. Shanab, R. Hafez, A. E. El-Ansary","doi":"10.21608/ejbo.2023.155080.2078","DOIUrl":"https://doi.org/10.21608/ejbo.2023.155080.2078","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42049325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-21DOI: 10.21608/ejbo.2023.171065.2179
K. Farghali
T HIS STUDY deals with the effects of tri-factorial interaction on some traits (elongation; weight and biomass accumulation efficiency of roots) and anatomical features of seedling Simmondsia chinensis roots. The obtained data indicated that, the moderate sodicity affected the root length which was enhanced by the sub optimal and optimal temperatures with low salinity stress. The decreased root elongation was inhibited under supra optimal temperature with decreased Ψ s . Statistically, the Ψ s had a dominant role while temperature effect was secondary on the root length and weight. It was found that, the temperature ranges from 20 to 28 was favorable for well development of the root anatomical structures and the root hairs formation, particular at low osmotic stress and moderate sodicity. Adversely, the elevated temperature had a deleterious effect on the root circulation which caused a wider vascular cylinder with narrower root cortex, as well as a distortion {lysis} of parenchymatous cells. The effect of the investigated factors and their combinations on the efficiency of cortical layer and xylem vessels was discussed.
{"title":"The Effect of Sodium Ions on the Anatomical Structure and Growth of Early Simmondsia chinensis (Link) C.K. Schneid. Roots under Reduced Water Potential and Temperature","authors":"K. Farghali","doi":"10.21608/ejbo.2023.171065.2179","DOIUrl":"https://doi.org/10.21608/ejbo.2023.171065.2179","url":null,"abstract":"T HIS STUDY deals with the effects of tri-factorial interaction on some traits (elongation; weight and biomass accumulation efficiency of roots) and anatomical features of seedling Simmondsia chinensis roots. The obtained data indicated that, the moderate sodicity affected the root length which was enhanced by the sub optimal and optimal temperatures with low salinity stress. The decreased root elongation was inhibited under supra optimal temperature with decreased Ψ s . Statistically, the Ψ s had a dominant role while temperature effect was secondary on the root length and weight. It was found that, the temperature ranges from 20 to 28 was favorable for well development of the root anatomical structures and the root hairs formation, particular at low osmotic stress and moderate sodicity. Adversely, the elevated temperature had a deleterious effect on the root circulation which caused a wider vascular cylinder with narrower root cortex, as well as a distortion {lysis} of parenchymatous cells. The effect of the investigated factors and their combinations on the efficiency of cortical layer and xylem vessels was discussed.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42510529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-21DOI: 10.21608/ejbo.2023.176949.2202
Soukayna Boudik, H. Khamar, N. Magri, N. Belahbib, L. Zidane, O. Benkhnigue, J. Dahmani
{"title":"Study of the Floristic Diversity of the Moulay Driss Zerhoun Region in the Prerif Range, Morocco","authors":"Soukayna Boudik, H. Khamar, N. Magri, N. Belahbib, L. Zidane, O. Benkhnigue, J. Dahmani","doi":"10.21608/ejbo.2023.176949.2202","DOIUrl":"https://doi.org/10.21608/ejbo.2023.176949.2202","url":null,"abstract":"","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":"1 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41474720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}