European Journal of Translational Myology最新文献

Warm-up is a fundamental preparatory phase for optimizing performance, yet its acute effects may depend on the specificity of the protocol to the target task. This pilot study compared a General, mobility-based Warm-Up (GWU) with a low-intensity, bodyweight Movement-Specific Warm-Up (MSWU) replicating squat biomechanics on maximal isometric force and neuromuscular activation during isometric squat. Eight resistance-trained men (age 23.5 ± 1.2 years; height 182.9 ± 5.9 cm; body mass 84.3 ± 9.1 kg; 1RM back squat 146 ± 19 kg) completed two randomized, counterbalanced sessions. Each session included a standardized preliminary warm-up, baseline maximal isometric high-bar back squat at 90° knee flexion, and either the GWU or the MSWU, followed by a 2-min rest and reassessment. Peak force and surface EMG of vastus medialis (VM), vastus lateralis (VL), rectus femoris (RF), gluteus maximus (GMax), and biceps femoris (BF) were measured. Completion time did not differ between protocols (~6-7 min; p = 0.806). Peak force significantly decreased after the GWU (-3.8%; p = 0.004; d = 1.47) but was maintained following the MSWU (-1.9%; p = 0.138; d = 0.59). Between-protocol differences in peak force were not significant (p = 0.186; d = 0.52). No significant changes were observed in normalized GMax activity or total integrated EMG. These results indicate that, for isometric, task-specific performance, a brief movement-specific warm-up better preserves force-generating capacity than a general mobility routine of similar duration, emphasizing the importance of biomechanical specificity in warm-up design.

Objective: To analyze the effects of Dapagliflozin on cardiac myocyte injury markers and left ventricular diastolic function in patients with heart failure with preserved ejection fraction (HF-PEF) after percutaneous coronary intervention (PCI).

Methods: This study included 120 HF-PEF patients post-PCI treated at our hospital between May 2021 and May 2024. Patients were divided into two groups: conventional therapy (n=49) and Dapagliflozin plus conventional therapy (n=71). Serum cardiac troponin I (cTnI) and serum amyloid A (SAA) were measured. Left ventricular function was assessed by ejection fraction (EF) and E/e' ratio. The incidence of major adverse cardiovascular events (MACE) was also recorded.

Results: After treatment, the Dapagliflozin group showed significantly lower cTnI and SAA levels compared to the conventional group (P < 0.05). EF was higher and E/e' ratio lower in the Dapagliflozin group (P < 0.05). The incidence of MACE was reduced to 1.41% versus 12.24% in the conventional group (P < 0.05).

Conclusion: Dapagliflozin effectively reduces cardiac myocyte injury markers, improves left ventricular diastolic function, and lowers the incidence of MACE in HF-PEF patients after PCI, demonstrating significant translational benefits for cardiac muscle function.

Irisin, a myokine released by skeletal muscle during physical activity, has emerged as a key regulator of energy metabolism, cellular stress responses, and longevity pathways. While previous studies have focused on aged animal-models or pathological states, the long-term impact of early-life interventions on molecular aging pathways remains poorly understood. This study investigated whether early-life irisin administration and physical exercise could modulate the renal-expression of Klotho and HSP70-two hallmark genes of cellular protection and anti-aging in young adult NMRI mice. Animals underwent 8 weeks of resistance training, endurance training, or irisin injection. Plasma irisin was quantified via ELISA, and renal Klotho and HSP70 expression levels were assessed using qPCR and Western-blotting. All interventions significantly increased circulating irisin and upregulated Klotho and HSP70 at both transcriptional and protein levels, with resistance training inducing the most pronounced effects. A 20-month survival analysis showed a trend toward improved longevity in all intervention groups. These findings suggest that early-life exercise and irisin exposure may activate renoprotective and longevity-associated pathways before the onset of molecular aging, supporting their potential as preventive strategies in translational geroscience.

Parry-Romberg Syndrome (PRS) is a rare condition that can affect the musculoskeletal system, resulting in progressive motor disabilities. The existing literature provides limited evidence regarding physical therapy interventions for PRS. We present a case of a 69-year-old woman diagnosed with PRS with associated ataxia treated with a neurorehabilitative program aimed at improving motor function and balance. We administered various functional scales (SPPB, Tampa Scale of Kinesiophobia, Berg Balance Scale, and Tinetti Scale). Additionally, a standardized gait analysis was conducted in our Gait and Motion Laboratory. Following neurorehabilitative therapy, the patient exhibited significant positive changes in various motor outcomes, as demonstrated by improvements in scores on standardized tests (SPBB, Berg, TSK and Tinetti scales when compared pre- and post-treatment), as well as enhancements in biomechanical parameters and quality of life (FIM, SF-12). This case report suggests that neurorehabilitative therapy can be beneficial in improving motor function and balance in PRS patient, as well as other rare condition associated with disabilities.

With the present work, we aimed to investigate antimicrobial resistance and virulence gene patterns of Staphylococcus aureus in lactating patients with infectious mastitis and evaluate their potential impact on inflammatory myopathies of the lactating breast. Between January 2021 and April 2024, 158 lactating patients with culture-confirmed infectious mastitis were treated at Hangzhou Linping District Maternal and Child Health Hospital. Among these, 119 isolates were identified as S. aureus (82 MRSA, 37 MSSA). Antimicrobial susceptibility and virulence genes were analyzed. Muscle involvement was inferred indirectly from clinical presentation, including marked local induration, tenderness extending to deeper breast tissue, and reduced breast mobility. No imaging or biopsy was performed to confirm myopathic changes directly. S. aureus was the predominant pathogen. Both MRSA and MSSA showed high resistance to penicillin G, erythromycin, and Clindamycin, while all isolates were susceptible to nitrofurantoin, linezolid, vancomycin, and rifampicin. MRSA exhibited higher resistance than MSSA (p<0.05). Frequent resistance genes included aac(6')/aph(2''), blaZ, mecA, aph(3')-III, and qacA/B. Virulence genes hla, clfA, clfB, and fnbA were common; pvl was less frequent in MRSA (p<0.05). MRSA infections were associated with stronger local inflammation and increased clinical markers possibly related to muscle involvement, raising the possibility of an association with myopathic changes in lactating breast tissue. S. aureus, particularly MRSA, is the main pathogen in lactating mastitis. Specific virulence genes may influence the severity of local inflammation and myopathic changes, highlighting implications for inflammatory myopathies in the lactating breast.

Stress urinary incontinence is common during and after pregnancy and impacts women's health both physically and mentally. This protocol suggests a non-invasive preemptive training strategy which integrates pelvic floor muscle training and core stability exercises with nutrition counseling. This model provides a clear, systematic, evidence-based framework which can be incorporated into antenatal care arising from a literature and clinical guideline review. The objective is to equip healthcare professionals with tangible, multidisciplinary strategies for proactive urinary incontinence care during in pregnancy, this model underscores the importance of early, non-pharmacological interventions in preserving pelvic health and preventing postpartum dysfunction, offering a feasible and low-cost strategy to support the well-being of pregnant women.

Retraction: Rafiee B, Karbalay-Doust S, Tabei SMB, Azarpira N, Alaee S, Lohrasbi P, Bahmanpour S. Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary. Eur J Transl Myol 2022; 32(2):10409. DOI: 10.4081/ejtm.2022.10409   The article has been retracted by the authors, in agreement with the Editor-in-Chief, Professor Ugo Carraro, and the Publisher, following an investigation into Figure 6 (specifically panels a and c). Although the image in question was not intentionally manipulated to mislead, and the error stemmed from an inadvertent mistake during figure preparation, the authors fully recognize the seriousness of the issue and the importance of maintaining the highest standards of scientific integrity. The authors apologize for this oversight and for any inconvenience it may have caused to the editorial board, reviewers, and readers, and are committed to learning from this experience and reinforcing stricter internal review processes to prevent such issues in future publications.

Beyond respiration, the diaphragm contributes to trunk stability and posture. Rehabilitative Ultrasound Imaging (RUSI) assesses Diaphragm Thickness (DT) and Excursion (DE), but evidence on reliability across novice examiners is limited. This study investigated intra- and inter-tester reliability of diaphragmatic RUSI across novice examiners in healthy women and compared DT and DE between parous and nulliparous participants. Healthy parous and nulliparous women participated. Exclusion criteria included pregnancy, chronic conditions, and BMI > 30 kg/m2. Two physiotherapists, novices in RUSI, measured DT during full inspiration and expiration and DE during quiet breathing. An independent examiner calculated the Thickening Fraction (TF). Three within-session measurements were taken for intra-tester reliability, whereas, for inter-tester reliability, agreement between mean measurements was analyzed via Intraclass Correlation Coefficients (ICC) and 95% Confidence Intervals (CIs). Independent samples t-tests assessed DT and DE differences between groups. Twenty women (33±14.3 years, BMI: 23.4±3.3) participated. Intra-tester reliability was moderate to good for DT (ICC:0.511-0.691) and very good for DE (ICC:0.700-0.756). Inter-tester reliability was very good for DE (ICC=0.748), and moderate for DT (ICC: 0.573-0.706). TF reliability was poor. Only one tester found higher inspiratory DT in parous women (p<0.01). Novice examiners can reliably measure DT and DE using RUSI, supporting its use in rehabilitation. Further standardization is required to enhance RUSI practices and additional research to clarify parity-related diaphragm changes.

We read with interest the article by ElZamarany et al. on a case-control study of the use of concentric needle macroelectromyography (CN-macroEMG) in 26 patients with Inflammatory Myopathies (IM) and 20 healthy control subjects.1 The study concludes that CN macroEMG provides optimal results in the diagnosis of IM and can be helpful in the follow-up of IM patients.1 The study is impressive, but some points should be discussed. The first point is that the objectives of the study were not specified.1 We should know whether the study was conducted to calculate the sensitivity and specificity of the method for diagnosing IM, or simply to document that CN-macroEMG may be abnormal in patients with IM. If the aim was to calculate sensitivity/specificity, we should know which gold standard for the diagnosis of IM was used to compare it with the CN-macroEMG findings. [...].

I was interested to read the article by Dowling et al.1 in the European Journal of Translational Myology, where the authors provide a comprehensive review of serum biomarkers for Duchenne Muscular Dystrophy (DMD), a devastating early-onset muscle wasting disease. The emphasis of research on liquid biopsy-based approaches, with the application of Mass Spectrometry (MS)-based proteomics for the identification of novel biomarkers like carbonic anhydrase CA3, fatty acid binding protein FABP3, and titin fragments, represents a revolutionary step towards minimally invasive diagnostics. As a neurologist and clinical pharmacologist working in Uganda, where diagnosis of DMD is delayed owing to the limited access to invasive tests, I find the prospects of these biofluid-based markers not only intriguing but also of immense worth in advancing precision medicine in resource-poor settings. [...].