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Effects of various disinfectants on surface roughness and color stability of thermoset and 3D-printed acrylic resin. 各种消毒剂对热固性和 3D 打印丙烯酸树脂表面粗糙度和颜色稳定性的影响。
IF 2.2 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-01-18 DOI: 10.4081/ejtm.2024.11701
Farnoush Fotovat, Samaneh Abbasi, Saeed Nikanjam, Behnaz Alafchi, Mandana Baghiat

Denture cleansers are extensively utilized to inhibit the colonization of various Candida species. Currently, additive technology in denture fabrication has become more prevalent. This study aims to assess the impact of disinfectants on the surface roughness and color stability of distinct denture bases. Disc-shaped samples (N=66) were exposed to three different disinfectants: 0.5% sodium hypochlorite, 1% hydrogen peroxide, and 2% chlorhexidine. The samples underwent evaluation via spectrophotometry and profilometry, respectively. Data analysis was conducted utilizing analysis of variance (ANOVA) (p < 0.05). Within the heat-cured group, sodium hypochlorite resulted in the most notable change in surface roughness (0.2 μm), while chlorhexidine exhibited the least impact (0.001 μm), showing a significant difference (p <0.008). The color change (ΔE) for 3D-printed samples immersed in all disinfectants was higher compared to heat-cured samples. Among the heat-cured samples, chlorhexidine induced the highest ΔE (2.76), while sodium hypochlorite resulted in the lowest (ΔE = 1.44), and this difference was statistically significant (p <0.008). Chlorhexidine caused the most significant color alteration among the solutions, while sodium hypochlorite induced the most considerable changes in surface roughness.

义齿清洁剂被广泛用于抑制各种念珠菌的定植。目前,添加剂技术在义齿制作中越来越普遍。本研究旨在评估消毒剂对不同义齿基托表面粗糙度和颜色稳定性的影响。圆盘状样品(N=66)暴露于三种不同的消毒剂中:0.5% 次氯酸钠、1% 过氧化氢和 2% 洗必泰。样品分别通过分光光度法和轮廓测定法进行评估。数据分析采用方差分析法(P < 0.05)。在热固化组中,次氯酸钠对表面粗糙度的影响最大(0.2 μm),而洗必泰对表面粗糙度的影响最小(0.001 μm),两者差异显著(p<0.05)。
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引用次数: 0
Efficacy and safety of Apixaban for the prevention of thrombosis in arteriovenous grafts. 阿哌沙班预防动静脉移植物血栓形成的有效性和安全性。
IF 2.2 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-01-15 DOI: 10.4081/ejtm.2024.12029
Arash Hedayat, Aidin Esrafilian Soltani, Mahdi Hakiminezhad, Fateme Zareian, Mandana Saneian, Mohamad Moradmand, Sahand Abrishami, Mohamad Hosein Tabatabaei Nodoushan, Ali Pouriayevali, Mahdi Mohebbi, Helia Ghorbani

This research aims to fill a vital gap in existing studies by evaluating the efficacy and safety of Apixaban, a direct oral anticoagulant, in the prevention of arteriovenous graft (AVG) thrombosis, thereby offering substantial insights into alternative anticoagulant options for hemodialysis patients. Conducted as a multi-center, randomized, double-blind, placebo-controlled trial, this study involved end-stage renal disease (ESRD) patients who had recently received polytetrafluoroethylene grafts. Participants were assigned to receive either Apixaban at a dose of 2.5 mg twice daily or a placebo. The primary outcome measure was the occurrence of graft thrombosis, while secondary outcomes focused on the incidence and severity of bleeding. Analytical methods included Kaplan-Meier estimates, Cox proportional hazards models, and conventional statistical tests. With 96 patients enrolled, the study found that Apixaban significantly reduced the incidence of AVG thrombosis compared to placebo (16.7% vs. 62.5%, P < 0.0001). Notably, this reduction in thrombosis incidence was not accompanied by an increase in bleeding events, thus affirming the safety profile of Apixaban as established in prior research. Apixaban is identified as an efficacious alternative to traditional anticoagulants in the prevention of AVG thrombosis among hemodialysis patients, representing a notable advancement in the care of individuals with ESRD. The results of this study support further investigations into the optimal dosing strategies specifically tailored for this patient demographic.

这项研究旨在通过评估阿哌沙班(一种直接口服抗凝剂)在预防动静脉移植物(AVG)血栓形成方面的有效性和安全性,填补现有研究的一个重要空白,从而为血液透析患者选择其他抗凝剂提供重要启示。该研究以多中心、随机、双盲、安慰剂对照试验的形式进行,参与者包括最近接受过聚四氟乙烯移植物治疗的终末期肾病(ESRD)患者。参与者被分配接受阿哌沙班治疗,剂量为 2.5 毫克,每天两次或安慰剂。主要结果指标是移植物血栓形成的发生率,次要结果指标是出血的发生率和严重程度。分析方法包括卡普兰-梅耶估计、Cox比例危险模型和常规统计检验。研究发现,与安慰剂相比,阿哌沙班能显著降低 AVG 血栓形成的发生率(16.7% 对 62.5%,P < 0.0001)。值得注意的是,血栓形成发生率的降低并没有伴随出血事件的增加,从而肯定了阿哌沙班在先前研究中确立的安全性。阿哌沙班被认为是预防血液透析患者动静脉瘘血栓形成的传统抗凝剂的有效替代品,代表着 ESRD 患者护理的显著进步。这项研究的结果支持进一步研究专门针对这一患者群体的最佳剂量策略。
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引用次数: 0
A patient with bilateral neuralgic amyotrophy and hepatitis E virus infection: an educational communication. 一名双侧神经性肌萎缩和戊型肝炎病毒感染的患者:教育交流。
IF 2.2 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-01-15 DOI: 10.4081/ejtm.2024.12143
Bojan Rojc, Nejc Šarabon

Neuralgic Amyotrophy, a peripheral nervous system disorder, is characterized by severe pain and muscle weakness, which can have a significant impact on patients' quality of life. The exact cause of neuralgic amyotrophy is unknown, but it may be linked to immunopathological mechanisms. Recent research has found an association between neuralgic amyotrophy and hepatitis E virus infection. This communication aims to expand knowledge on the clinical phenotype of patients with neuralgic amyotrophy and hepatitis E virus infection, presenting the case of a 55-year-old man diagnosed with bilateral neuralgic amyotrophy and hepatitis E virus infection.

神经性肌营养不良是一种周围神经系统疾病,以剧烈疼痛和肌肉无力为特征,对患者的生活质量有很大影响。神经性肌营养不良症的确切病因尚不清楚,但可能与免疫病理机制有关。最近的研究发现,神经性肌营养不良与戊型肝炎病毒感染有关。这篇通讯旨在扩大对神经性肌营养不良和戊型肝炎病毒感染患者临床表型的了解,介绍了一名被诊断为双侧神经性肌营养不良和戊型肝炎病毒感染的 55 岁男性病例。
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引用次数: 0
Sensorimotor and proprioceptive exercise programs to improve balance in older adults: a systematic review with meta-analysis. 提高老年人平衡能力的感知运动和本体感觉运动项目:系统回顾与荟萃分析。
IF 2.2 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-01-11 DOI: 10.4081/ejtm.2024.12010
Silvo Pšeničnik Sluga, Ziga Kozinc

The primary aim of this study was to systematically review and meta-analyze the impact of sensorimotor and proprioceptive exercises on balance in older adults. We also sought to define how researchers describe proprioceptive and sensory-motor training and their respective protocols. The review was conducted following the PRISMA guidelines, with searches performed in March 2023. Both authors carried out independent searches using the PubMed and PEDro databases. From a total of 320 identified records, 12 studies were deemed eligible for meta-analysis after screening and removal of duplicates. The average PEDro score was 5.11 ± 1.11 indicating overall fair quality of studies.  Common outcome measures included the Berg balance scale, Timed up and go test, Tinetti balance scale, Functional reach test and various single-leg stance tests. All outcomes were significantly improved by the interventions (standard mean difference = 0.65 - 1.29), with little difference between proprioceptive and sensorimotor training. However, the quality of evidence ranged from "very low" to "low" based on GRADE guidelines, suggesting further high-quality studies are needed. This review underscores the potential benefits of sensorimotor and proprioceptive exercises for enhancing balance in older adults, while also highlighting the ambiguity and inconsistency regarding the usage of the terms proprioceptive and sensorimotor training.

本研究的主要目的是系统回顾和元分析感觉运动和本体感觉训练对老年人平衡能力的影响。我们还试图确定研究人员如何描述本体感觉和感觉运动训练及其各自的方案。综述按照 PRISMA 指南进行,检索时间为 2023 年 3 月。两位作者使用 PubMed 和 PEDro 数据库进行了独立检索。在总共 320 条已确认的记录中,经过筛选和去除重复内容后,有 12 项研究被认为符合荟萃分析的条件。PEDro 平均得分为 5.11 ± 1.11,表明研究的总体质量尚可。 常见的结果测量包括伯格平衡量表、计时起立和走动测试、蒂内蒂平衡量表、功能性伸展测试和各种单腿站立测试。所有结果都在干预措施的作用下得到了明显改善(标准平均差 = 0.65 - 1.29),本体感觉和感觉运动训练之间的差异很小。然而,根据 GRADE 指南,证据质量从 "很低 "到 "低 "不等,这表明还需要进一步的高质量研究。本综述强调了感觉运动和本体运动训练对增强老年人平衡能力的潜在益处,同时也强调了本体感觉和感觉运动训练这两个术语在使用上的模糊性和不一致性。
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引用次数: 0
Summaries of the Padua symposium on myofascial pain, fibromyalgia, and fascial pain disorders, June 2023, Aula Falloppio at the Human Anatomy Institute of the University of Padova, Italy 帕多瓦肌筋膜疼痛、纤维肌痛和筋膜疼痛疾病研讨会摘要,2023 年 6 月,意大利帕多瓦大学人体解剖研究所 Aula Falloppio
IF 2.2 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-12-22 DOI: 10.4081/ejtm.2023.12194
Robert Gerwin, A. Stecco, Carla Stecco, James Fricton
The International Symposium on Myofascial Pain, Fibromyalgia, and Fascial Pain Disorders was held at the University of Padua, Padua, Italy in June of 2023. This report presents a summary of the presentations from scientists and clinicians from around the world who presented to the symposium. The purpose of this symposium and resultant paper is improve health professional’s recognition and understanding of the clinical characteristics, co-morbidities, mechanisms, and treatment strategies for these common conditions to better understand and manage their pain, dysfunction, and quality life.
肌筋膜疼痛、纤维肌痛和筋膜疼痛疾病国际研讨会于 2023 年 6 月在意大利帕多瓦的帕多瓦大学举行。本报告总结了来自世界各地的科学家和临床医生在研讨会上的发言。本次研讨会和论文的目的是提高医疗专业人员对这些常见疾病的临床特征、并发症、机制和治疗策略的认识和理解,从而更好地了解和管理他们的疼痛、功能障碍和生活质量。
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引用次数: 0
Pax7 reporter mouse models: a pocket guide for satellite cell research. Pax7 报告小鼠模型:卫星细胞研究袖珍指南。
IF 1.8 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-12-18 DOI: 10.4081/ejtm.2023.12174
Huascar Pedro Ortuste Quiroga, Shin Fujimaki, Yusuke Ono

Since their discovery, satellite cells have showcased their need as primary contributors to skeletal muscle maintenance and repair. Satellite cells lay dormant, but when needed, activate, differentiate, fuse to fibres and self-renew, that has bestowed satellite cells with the title of muscle stem cells. The satellite cell specific transcription factor Pax7 has enabled researchers to develop animal models against the Pax7 locus in order to isolate and characterise satellite cell-mediated events. This review focuses specifically on describing Pax7 reporter mouse models. Here we describe how each model was generated and the key findings obtained. The strengths and limitations of each model are also discussed. The aim is to provide new and current satellite cell enthusiasts with a basic understanding of the available Pax7 reporter mice and hopefully guide selection of the most appropriate Pax7 model to answer a specific research question.

自卫星细胞被发现以来,它们一直是骨骼肌维护和修复的主要贡献者。卫星细胞处于休眠状态,但在需要时会激活、分化、融合成纤维并自我更新,因此卫星细胞被称为肌肉干细胞。卫星细胞特异性转录因子 Pax7 使研究人员能够针对 Pax7 基因座开发动物模型,以分离和描述卫星细胞介导的事件。本综述重点介绍 Pax7 报告小鼠模型。在此,我们将介绍每种模型的产生过程和主要发现。同时还讨论了每种模型的优势和局限性。目的是让新的和现有的卫星细胞爱好者对现有的 Pax7 报告小鼠有一个基本的了解,并希望指导他们选择最合适的 Pax7 模型来回答特定的研究问题。
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引用次数: 0
Five Padua days on muscle and mobility medicine (2024Pdm3) 27 February - 2 March, 2024 at Hotel Petrarca, Thermae of Euganean Hills, Padua, and San Luca Hall, Prato della Valle, Padua, Italy. 帕多瓦肌肉与活动医学五日(2024Pdm3),意大利帕多瓦。
IF 1.8 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-12-18 DOI: 10.4081/ejtm.2023.12161
Sandra Zampieri, Ines Bersch, Piera Smeriglio, Elena Barbieri, Massimo Ganassi, Christiaan Leeuwenburg, Riccardo Rosati, Paolo Gargiulo, Amber Pond, H Lee Sweeney, Ugo Carraro

At the end of the 2023 Padua Days of Muscle and Mobility Medicine the next year's meeting was scheduled from 27 February to 2 March 2024 (2024Pdm3). During the summer and autumn the program was confirmed with Scientific Sessions that will take place over five days, starting in the afternoon of February 27, 2024 at the Conference Room of the Hotel Petrarca, Thermae of Euganean Hills (Padua), Italy. As usual, the next day will be spent in Padua, in this occasion at the San Luca Hall of the Santa Giustina monastery in Prato della Valle, Padua, Italy. Collected during Autumn 2023, many more titles and abstracts than expected were submitted, forcing the organization of parallel sessions both on March 1 and March 2 2024 confirming attractiveness of the 2024 Pdm3. The five days will include oral presentations of scientists and clinicians from Argentina, Austria, Belgium, Brazil, Canada, Denmark, Egypt, France, Germany, Iceland, Ireland, Italy, Romania, Russia, Slovenia, Switzerland, UK and USA. Together with the preliminary Program at December 1, 2023, the early submitted Abstracts is e-published in this Issue 33 (4) 2023 of the European Journal of Translational Myology (EJTM). You are invited to join, submitting your Last Minute Abstracts to ugo.carraro@unipd.it by February 1, 2024. Furthermore, with the more generous deadline of May 20, 2024, submit please "Communications" to the European Journal of Translational Myology (Clarivate's ESCI Impact factor 2.2; SCOPUS Cite Score: 3.2). See you soon at the Hotel Petrarca in Montegrotto Terme, Padua, on February 27, 2024, but the complete program can be followed from home via zoom connection.

2023 年帕多瓦肌肉与运动医学日活动结束后,下一年的会议定于 2024 年 2 月 27 日至 3 月 2 日举行(2024Pdm3)。在夏季和秋季,会议日程已确定,科学会议将举行五天,从 2024 年 2 月 27 日下午开始,地点在意大利帕多瓦欧加尼山温泉酒店(Hotel Petrarca, Thermae of Euganean Hills)会议室。按照惯例,第二天将在帕多瓦度过,这次将在意大利帕多瓦市普拉托德拉瓦莱的圣朱斯蒂纳修道院的圣卢卡厅举行。2023 年秋季收集到的论文标题和摘要比预期的要多得多,因此不得不在 2024 年 3 月 1 日和 3 月 2 日组织平行会议,这也证实了 2024 年 Pdm3 的吸引力。为期五天的会议将包括来自阿根廷、奥地利、比利时、巴西、加拿大、丹麦、埃及、法国、德国、冰岛、爱尔兰、意大利、罗马尼亚、俄罗斯、斯洛文尼亚、瑞士、英国和美国的科学家和临床医生的口头报告。连同 2023 年 12 月 1 日的初步计划,早期提交的摘要将在本期《欧洲转化肌病学杂志》(EJTM)第 33 (4) 2023 期上以电子版形式发布。诚邀您加入,在 2024 年 2 月 1 日前将您的最后一分钟摘要提交至 ugo.carraro@unipd.it。此外,请在 2024 年 5 月 20 日这一更宽松的截止日期前向《欧洲转化肌病学杂志》提交 "通讯"(Clarivate 的 ESCI 影响因子为 2.2;SCOPUS 引用评分:3.2)。我们将于 2024 年 2 月 27 日在帕多瓦蒙特格罗托泰尔梅的佩特拉卡酒店(Hotel Petrarca)相见,但您也可以在家中通过变焦连接观看完整的会议日程。
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引用次数: 0
Platelets-rich-plasma in management of non operative post cruciate ligament injury. 血小板-血浆在非手术治疗十字韧带损伤后的应用。
IF 1.8 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-12-18 DOI: 10.4081/ejtm.2023.11535
Giuseppe Messina, Pietro Cataldo, Francesco Mantia, Eleonora Maria Iovane, Carlotta Mantia, Micol Terrasi, Angelo Iovane

Posterior cruciate ligament (PCL) injury is a rare ligamentous knee injury which most commonly occurs due to direct trauma; it represents only 0.65% of knee injuries. On the basis of magnetic resonance imaging (MRI), PCL injury is divided into surgical and non-surgical. In the last few years we have witnessed an increasing interest in regenerative medicine. The use of innovative techniques allow the reduction of rehabilitation times in sports injuries. Platelet-Rich-Plasma (PRP) is a plasma-derived which stimulate the tissue repair. The objective of our work is to compare the rehabilitation times in PCL non-operative injuries treated with standard conservative treatment and PCL injuries treated with autologous PRP followed by rehabilitation program. We divided patients into two different groups: Group A formed by patients who underwent a standard conservative rehabilitation program and Group B which included patients treated by ultrasound guided PRP injection and following physiotherapy program. Both groups of patients resumed sports activities; however the rehabilitation times for patients which had undergone treatment with autologous PRP injection were reduced compared to patients who underwent a standard conservative treatment.

膝关节后交叉韧带(PCL)损伤是一种罕见的膝关节韧带损伤,最常见于直接外伤,仅占膝关节损伤的 0.65%。根据磁共振成像(MRI),PCL损伤分为手术和非手术两种。在过去几年中,我们看到人们对再生医学的兴趣与日俱增。创新技术的使用缩短了运动损伤的康复时间。富血小板血浆(PRP)是一种可刺激组织修复的血浆。我们的研究目的是比较 PCL 非手术损伤患者接受标准保守治疗和 PCL 损伤患者接受自体 PRP 治疗后进行康复计划所需的康复时间。我们将患者分为两组:A 组为接受标准保守康复治疗的患者,B 组为接受超声引导 PRP 注射治疗并接受物理治疗的患者。两组患者都恢复了体育活动,但与接受标准保守治疗的患者相比,接受自体血浆蛋白注射治疗的患者的康复时间更短。
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引用次数: 0
ChatGPT in the development of medical questionnaires. The example of the low back pain. ChatGPT 在医疗调查问卷开发中的应用。以腰背痛为例。
IF 1.8 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-12-15 DOI: 10.4081/ejtm.2023.12114
Daniele Coraci, Maria Chiara Maccarone, Gianluca Regazzo, Giorgia Accordi, Jannis V Papathanasiou, Stefano Masiero

In the last year, Chat Generative Pre-Trained Transformer (ChatGPT), a web software based on artificial intelligence has been showing high potential in every field of knowledge. In the medical area, its possible application is an object of many studies with promising results. We performed the current study to investigate the possible usefulness of ChatGPT in assessing low back pain. We asked ChatGPT to generate a questionnaire about this clinical condition and we compared the obtained questions and results with the ones obtained by other validated questionnaires: Oswestry Disability Index, Quebec Back Pain Disability Scale, Roland-Morris Disability Questionnaire, and Numeric Rating Scale for pain. We enrolled 20 subjects with low back pain and we found important consistencies among the validated questionnaires. The ChatGPT questionnaire showed an acceptable significant correlation only with Oswestry Disability Index and Quebec Back Pain Disability Scale. ChatGPT showed some peculiarities, especially in the assessment of quality of life and medical consultation and treatments. Our study shows that ChatGPT can help evaluate patients, including multilevel perspectives. However, its power is limited, and further research and validation are required.

去年,基于人工智能的网络软件 Chat Generative Pre-Trained Transformer(ChatGPT)在各个知识领域都显示出巨大的潜力。在医学领域,许多研究都对其可能的应用进行了探讨,并取得了可喜的成果。我们目前的研究旨在调查 ChatGPT 在评估腰背痛方面可能发挥的作用。我们要求 ChatGPT 生成一份有关该临床症状的问卷,并将获得的问题和结果与其他有效问卷进行比较:这些问卷包括:Oswestry 残疾指数、魁北克背痛残疾量表、Roland-Morris 残疾问卷和疼痛数字评定量表。我们招募了 20 名腰背痛受试者,发现这些有效问卷之间存在重要的一致性。ChatGPT 问卷仅与 Oswestry 残疾指数和魁北克背痛残疾量表有可接受的显著相关性。ChatGPT 显示了一些特殊性,尤其是在生活质量评估和医疗咨询与治疗方面。我们的研究表明,ChatGPT 可以帮助评估患者,包括多层次的视角。然而,它的作用有限,需要进一步研究和验证。
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引用次数: 0
Angiotensin-(1-7) improves skeletal muscle regeneration. 血管紧张素-(1-7)可改善骨骼肌再生。
IF 1.8 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2023-12-15 DOI: 10.4081/ejtm.2023.12037
Mayalen Valero-Breton, Franco Tacchi, Johanna Abrigo, Felipe Simon, Daniel Cabrera, Claudio Cabello-Verrugio

Skeletal muscle possesses regenerative potential via satellite cells, compromised in muscular dystrophies leading to fibrosis and fat infiltration. Angiotensin II (Ang-II) is commonly associated with pathological states. In contrast, Angiotensin (1-7) [Ang-(1-7)] counters Ang-II, acting via the Mas receptor. While Ang-II affects skeletal muscle regeneration, the influence of Ang-(1-7) remains to be elucidated. Therefore, this study aims to investigate the role of Ang-(1-7) in skeletal muscle regeneration. C2C12 cells were differentiated in the absence or presence of 10 nM of Ang-(1-7). The diameter of myotubes and protein levels of myogenin and myosin heavy chain (MHC) were determined. C57BL/6 WT male mice 16-18 weeks old) were randomly assigned to injury-vehicle, injury-Ang-(1-7), and control groups. Ang-(1-7) was administered via osmotic pumps, and muscle injury was induced by injecting barium chloride to assess muscle regeneration through histological analyses. Moreover, embryonic myosin (eMHC) and myogenin protein levels were evaluated. C2C12 myotubes incubated with Ang-(1-7) showed larger diameters than the untreated group and increased myogenin and MHC protein levels during differentiation. Ang-(1-7) administration enhances regeneration by promoting a larger diameter of new muscle fibers. Furthermore, higher numbers of eMHC (+) fibers were observed in the injured-Ang-(1-7), which also had a larger diameter. Moreover, eMHC and myogenin protein levels were elevated, supporting enhanced regeneration due to Ang-(1-7) administration. Ang-(1-7) effectively promotes differentiation in vitroand improves muscle regeneration in the context of injuries, with potential implications for treating muscle-related disorders.

骨骼肌具有通过卫星细胞再生的潜力,但在肌肉萎缩症中会受到损害,导致纤维化和脂肪浸润。血管紧张素 II(Ang-II)通常与病理状态有关。相比之下,血管紧张素(1-7)[Ang-(1-7)]则通过 Mas 受体与 Ang-II 对抗。虽然 Ang-II 会影响骨骼肌再生,但 Ang-(1-7) 的影响仍有待阐明。因此,本研究旨在探讨 Ang-(1-7) 在骨骼肌再生中的作用。在没有或存在 10 nM Ang-(1-7) 的情况下分化 C2C12 细胞。测定肌管的直径以及肌原蛋白和肌球蛋白重链(MHC)的蛋白水平。将 16-18 周大的 C57BL/6 WT 雄性小鼠随机分配到损伤-车辆组、损伤-Ang-(1-7)组和对照组。通过渗透泵注射 Ang-(1-7),并通过注射氯化钡诱导肌肉损伤,通过组织学分析评估肌肉再生情况。此外,还评估了胚胎肌球蛋白(eMHC)和肌原蛋白的水平。与未处理组相比,用 Ang-(1-7) 培养的 C2C12 肌细胞管直径更大,分化过程中的肌原蛋白和 MHC 蛋白水平也有所提高。Ang-(1-7)能促进新肌纤维直径增大,从而增强再生能力。此外,在损伤-Ang-(1-7)组中观察到更多的 eMHC(+)纤维,其直径也更大。此外,eMHC和肌原蛋白水平升高,这也支持了Ang-(1-7)用药后再生能力的增强。Ang-(1-7)能有效促进体外分化,改善受伤肌肉的再生,对治疗肌肉相关疾病具有潜在意义。
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引用次数: 0
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European Journal of Translational Myology
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