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Recuperación mejorada tras la cirugía y resultados oncológicos a largo plazo: análisis post hoc del estudio POWER 改善术后康复和长期肿瘤结果:POWER研究的事后分析
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-11-01 DOI: 10.1016/j.redar.2025.501926
J. Ripollés-Melchor , A. Zorrilla-Vaca , A. León-Bretscher , A. Abad-Motos , P. Galán-Menéndez , J.L. Muñoz-Rodés , D. Zapata , Á. Ramiro-Ruiz , C. Aldecoa , V. Molnar , L. Jordá-Sanz , A. Cuéllar-Martínez , H. Berges-Gutiérrez , A. Landaluce-Olavarria , R. Fernández-García , N. Aracil-Escoda , A.M. Pacual-Bellosta , M. Ubré-Lorenzo , M. Logroño-Ejea , A.B. Serrano Romero , A. Abad-Gurumeta

Introduction/Objectives

Colorectal cancer (CRC) remains a leading cause of cancer-related mortality. While Enhanced Recovery After Surgery (ERAS) programs optimize perioperative care, their effect on oncologic prognosis requires further validation. This study evaluates ERAS adherence and five-year survival through a post-hoc analysis of the POWER Study.

Methods

This sub-analysis included 901 patients from 32 hospitals with available five-year follow-up data. Patients undergoing elective CRC resection were stratified based on ERAS adherence (≥ 70%). Primary outcomes included overall survival and recurrence rates, analyzed using Cox proportional hazards models adjusted for clinical variables. Kaplan-Meier curves and subgroup analyses were also performed to assess stage-specific differences.

Results

No significant differences were observed in five-year overall survival (ERAS 66% vs. non-ERAS 60%; HR: 1.14, 95% CI: 0.88-1.49; P = 0.32) or recurrence rates (ERAS 25% vs. non-ERAS 25%; HR: 0.91, 95% CI: 0.68-1.22; P = 0.53). Kaplan-Meier curves showed overlapping survival trajectories, and subgroup analyses confirmed no stage-specific disparities. While ERAS promotes early postoperative recovery and facilitates timely adjuvant therapy initiation, its effect on long-term oncologic outcomes remains inconclusive.

Conclusions

These findings support ERAS as a safe perioperative strategy that enhances short-term recovery without compromising oncologic safety. However, limitations such as the post-hoc design and incomplete long-term data warrant further research. Future studies should investigate ERAS's impact on perioperative stress, immune function, and recurrence prevention to better elucidate its role in long-term CRC outcomes.
结直肠癌(CRC)仍然是癌症相关死亡的主要原因。虽然ERAS计划优化了围手术期护理,但其对肿瘤预后的影响需要进一步验证。本研究通过对POWER研究的事后分析来评估ERAS的依从性和5年生存率。方法本亚组分析纳入32家医院901例患者,随访5年。接受选择性结直肠癌切除术的患者根据ERAS依从性(≥70%)进行分层。主要结局包括总生存率和复发率,使用经临床变量调整的Cox比例风险模型进行分析。Kaplan-Meier曲线和亚组分析也用于评估分期差异。结果5年总生存率(ERAS 66% vs.非ERAS 60%; HR: 1.14, 95% CI: 0.88-1.49; P = 0.32)或复发率(ERAS 25% vs.非ERAS 25%; HR: 0.91, 95% CI: 0.68-1.22; P = 0.53)无显著差异。Kaplan-Meier曲线显示重叠的生存轨迹,亚组分析证实没有特定阶段的差异。虽然ERAS可以促进术后早期恢复并促进及时的辅助治疗,但其对长期肿瘤预后的影响仍不确定。结论:这些发现支持ERAS作为一种安全的围手术期策略,可以在不影响肿瘤安全性的情况下提高短期恢复。然而,诸如事后设计和不完整的长期数据等限制需要进一步研究。未来的研究应探讨ERAS对围手术期应激、免疫功能和复发预防的影响,以更好地阐明其在CRC长期预后中的作用。
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引用次数: 0
Comparación entre el efecto de una única inyección de morfina neuroaxial y el bloqueo en el plano del músculo erector de la columna en la calidad de la recuperación (QoR-15) tras cirugías gastrointestinales mayores abiertas: ensayo aleatorizado prospectivo 单次神经轴吗啡注射与大开放性胃肠道手术后脊柱勃起肌阻塞对恢复质量(QoR-15)的影响比较:前瞻性随机试验
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-11-01 DOI: 10.1016/j.redar.2025.501925
A. Choudhary , S. Singh , V. Kumar , H. Kumar , K. Parasar

Background

Assessing a patient's quality of recovery after major surgery is crucial. This study aims to compare the effect of neuraxial morphine and bilateral erector spinae plane block on quality of recovery in the first 48 hours postoperative in patients undergoing open upper abdominal surgeries.

Methods

This prospective, triple-arm randomized study was conducted to compare the effect of neuraxial morphine (intrathecal morphine, thoracic epidural) with erector spinae plane block on postoperative recovery. The primary outcome was the Quality of Recovery-15 (QoR-15) score at 24 h. Secondary objectives were pain severity using the numerical rating scale (NRS) score during 24 h after surgery, time to first rescue analgesia, and adverse effects.

Results

We enrolled 117 adult patients undergoing open upper abdominal surgeries who were randomly allocated to the intrathecal morphine (ITM) group, thoracic epidural (TEM) group, or bilateral low volume erector spinae plane block (LV-ESPB) group. The mean ± standard deviation 24-h QoR-15 scores were comparable in the ITM [115.83 ± 4.0] and TEM [113.51 ± 4.36] groups; however, they were significantly decreased in the LV-ESPB group [104.58 ± 4.05] (p value < 0.001). All time points showed equivalent NRS scores except at 24 hours (p-value - 0.002) between the three groups. The time to first rescue analgesia was comparable in all three groups. The incidence of nausea and pruritus was statistically significant in the ITM group compared to the TEM and LV-ESPB groups (p-value < 0.001 and 0.019, respectively).

Conclusions

The study demonstrated that neuraxial morphine achieved better quality of recovery in the first 24 hours after major abdominal surgery as compared to bilateral erector spinae plane block.
背景:评估大手术后患者的康复质量是至关重要的。本研究旨在比较神经轴吗啡和双侧竖脊肌平面阻滞对开放性上腹部手术患者术后48小时恢复质量的影响。方法采用前瞻性、三组随机研究,比较神经轴向吗啡(鞘内吗啡、胸椎硬膜外吗啡)与竖脊肌平面阻滞对术后恢复的影响。主要结局是24小时的恢复质量-15 (QoR-15)评分。次要目标是术后24小时内使用数值评定量表(NRS)评分的疼痛严重程度、首次抢救镇痛时间和不良反应。结果117例接受开放性上腹部手术的成年患者被随机分为鞘内吗啡组(ITM)、胸硬膜外吗啡组(TEM)和双侧低容积竖脊肌平面阻滞组(LV-ESPB)。ITM组24小时QoR-15评分的平均值±标准差为115.83±4.0,TEM组为113.51±4.36;而LV-ESPB组明显降低[104.58±4.05](p值<; 0.001)。除24小时外,三组间各时间点NRS评分均相等(p值- 0.002)。三组患者首次抢救镇痛的时间具有可比性。与TEM和LV-ESPB组相比,ITM组恶心和瘙痒的发生率有统计学意义(p值分别为0.001和0.019)。结论与双侧竖脊肌平面阻滞相比,神经轴向吗啡在腹部大手术后24小时内的恢复质量更好。
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引用次数: 0
Toxina botulínica perineural en el tratamiento de la cefalea postraumática. Caso clínico 神经周围肉毒杆菌毒素治疗创伤后头痛。临床病例
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-11-01 DOI: 10.1016/j.redar.2025.501903
L. Arce Gálvez , J.M. Mancera Álzate
Post-traumatic headache is a common condition that can be managed with pharmacologic interventions or analgesic procedures; however, most evidence is derived from patients with mild trauma, leaving a large gap with regard to patients with moderate or severe trauma who present complex pain. Botulinum toxin plays an increasingly important role in pain management. This neurotoxin acts on different receptors, ranging from TRPV1 (transient receptor potential vanilloid type 1) to CGRP (calcitonin gene-related peptide). This is the first case report of the use of perineural botulinum toxin in a patient with moderate post-traumatic headache who responded poorly to standard interventional measures.
创伤后头痛是一种常见疾病,可通过药物干预或镇痛程序加以控制;然而,大多数证据来自轻度创伤患者,对于出现复杂疼痛的中度或重度创伤患者,存在很大的差距。肉毒杆菌毒素在疼痛管理中扮演着越来越重要的角色。这种神经毒素作用于不同的受体,从TRPV1(瞬时受体电位1型香草素)到CGRP(降钙素基因相关肽)。这是首例使用神经周肉毒杆菌毒素治疗中度创伤后头痛患者的病例报告,该患者对标准干预措施反应不佳。
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引用次数: 0
Impacto de los rellenos faciales en la valoración de la vía aérea 面部填充物对航线估值的影响
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-11-01 DOI: 10.1016/j.redar.2025.501802
C. Juliá-Romero , B. Simón-Rivero , L. Ceresuela-Dionís , S. de Benito-Mendieta
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引用次数: 0
Recomendaciones basadas en la opinión de expertos promovidas por GTIPO-SEDAR para optimizar el abordaje terapéutico de la candidiasis intraabdominal GTIPO-SEDAR根据专家意见提出的关于优化腹部念珠菌病治疗方法的建议
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-11-01 DOI: 10.1016/j.redar.2025.501916
E. Maseda , T. Peláez , G. Aguilar , I. Martín Loeches , A. Benítez-Cano , A. Rodríguez , R. Zaragoza , J. Guinea , C. Aldecoa , A. Suárez de la Rica
Intra-abdominal candidiasis (IAC) is a highly prevalent infectious syndrome and a significant cause of mortality in intensive care units. The Working Group on Perioperative Infections of the Spanish Society of Anesthesiology, Resuscitation, and Pain Management has promoted the development of recommendations based on the experience of a multidisciplinary panel of experts from various medical specialties, aiming to optimize the diagnosis and treatment of severe infectious processes such as IAC. These recommendations have been formulated through a rigorous critical appraisal of the currently available scientific evidence. This document provides guidance for optimizing the therapeutic approach to IAC, incorporating the latest findings in microbiology, recent therapeutic advances, pharmacokinetic and pharmacodynamic considerations in critically ill patients with IAC, special therapeutic considerations for patients undergoing extracorporeal membrane oxygenation and continuous renal replacement therapies, the appropriateness of empirical versus targeted therapy, and strategies for improving and reducing the duration of treatment.
腹腔内念珠菌病(IAC)是一种高度流行的感染综合征,也是重症监护病房死亡的重要原因。西班牙麻醉、复苏和疼痛管理学会围手术期感染工作组根据来自不同医学专业的多学科专家小组的经验,促进了建议的发展,旨在优化IAC等严重感染过程的诊断和治疗。这些建议是通过对现有科学证据进行严格的批判性评估而制定的。本文为优化IAC的治疗方法提供了指导,包括微生物学的最新发现、最近的治疗进展、危重IAC患者的药代动力学和药效学考虑、接受体外膜氧合和持续肾脏替代治疗的患者的特殊治疗考虑、经验治疗与靶向治疗的适宜性。以及改善和缩短治疗时间的策略。
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引用次数: 0
Abordaje abierto frente a abordaje ecográfico para el bloqueo profundo del plano del músculo serrato anterior para analgesia postoperatoria tras mastectomía radical modificada: ensayo aleatorizado controlado 改良根治性乳房切除术后,用超声方法对前锥形肌平面进行深度阻塞:一项随机对照试验
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-11-01 DOI: 10.1016/j.redar.2025.501902
M. Mohammed Alseoudy , M. Abd el-Ghaffar Saleh , S. Saleh Elbalka , T. Elmetwally Farahat , D. Abdellatif Elebedy , S. Elsayed Ahmed

Background and aims

Ultrasound-guided deep serratus anterior plane (SAP) block has recently gained popularity as an analgesic technique in breast surgery. However, the effectiveness of ultrasound depends largely on the quality of the equipment used, and the technique can be complicated by patient-related factors such as obesity. We hypothesized that the simpler open approach to deep SAP block would be non-inferior to the ultrasound-guided approach in providing analgesia for modified radical mastectomy.

Method

A non-inferiority, randomized controlled study was performed in 100 patients aged 18-60 years who underwent modified radical mastectomy. In the open approach group (n = 50), 30 ml of 0.25% bupivacaine was injected deep to the serratus anterior muscle after breast resection and rib palpation. In the ultrasound-guided group (n = 50), 30 ml of 0.25% bupivacaine was injected deep to the serratus anterior muscle under ultrasound guidance before the skin incision. The primary outcome was total morphine consumption in the first 24 postoperative hours. Secondary outcome measures included time to first request for rescue analgesia (duration of SAP block), postoperative visual analogue scale score, and the incidence of adverse effects.

Results

Median (interquartile range) morphine requirement in the first 24 h was similar in both groups (P = 0.81), and the time to first request for analgesia was also similar (P = 0.81). Evaluation of visual analogue scale scores during the initial 24 h after surgery showed no statistically significant differences between groups (P > 0.05), except at 4 postoperative hours, when the score was significantly higher in the ultrasound group (P < 0.001). No complications were observed in either group.

Conclusion

The open approach to deep SAP block is comparable to the ultrasound-guided approach in providing postoperative analgesia in female patients undergoing modified radical mastectomy.
背景与目的超声引导下深锯肌前平面(SAP)阻滞作为一种乳房手术镇痛技术最近得到了广泛的应用。然而,超声波的有效性在很大程度上取决于所使用设备的质量,而且该技术可能会因与患者相关的因素(如肥胖)而复杂化。我们假设,在改良乳房根治术中,更简单的开放入路对深部SAP阻滞的镇痛作用不逊于超声引导入路。方法对100例18-60岁行改良乳房根治术的患者进行非劣效性随机对照研究。开放入路组(n = 50)在切除乳房和触诊肋骨后,于前锯肌深层注射0.25%布比卡因30 ml。超声引导组(n = 50)在超声引导下,于皮肤切开前锯肌深层注射0.25%布比卡因30 ml。主要观察指标为术后24小时吗啡总消耗量。次要结局指标包括首次请求抢救镇痛的时间(SAP阻滞持续时间)、术后视觉模拟评分和不良反应发生率。结果两组患者前24 h吗啡需取量中位数(四分位间距)相似(P = 0.81),首次请求镇痛时间相似(P = 0.81)。术后24 h视觉模拟量表评分各组间差异无统计学意义(P > 0.05),但术后4 h超声组评分明显高于对照组(P < 0.001)。两组均无并发症发生。结论开放入路对女性改良乳房根治术患者深部SAP阻滞的镇痛效果与超声引导入路相当。
{"title":"Abordaje abierto frente a abordaje ecográfico para el bloqueo profundo del plano del músculo serrato anterior para analgesia postoperatoria tras mastectomía radical modificada: ensayo aleatorizado controlado","authors":"M. Mohammed Alseoudy ,&nbsp;M. Abd el-Ghaffar Saleh ,&nbsp;S. Saleh Elbalka ,&nbsp;T. Elmetwally Farahat ,&nbsp;D. Abdellatif Elebedy ,&nbsp;S. Elsayed Ahmed","doi":"10.1016/j.redar.2025.501902","DOIUrl":"10.1016/j.redar.2025.501902","url":null,"abstract":"<div><h3>Background and aims</h3><div>Ultrasound-guided deep serratus anterior plane (SAP) block has recently gained popularity as an analgesic technique in breast surgery. However, the effectiveness of ultrasound depends largely on the quality of the equipment used, and the technique can be complicated by patient-related factors such as obesity. We hypothesized that the simpler open approach to deep SAP block would be non-inferior to the ultrasound-guided approach in providing analgesia for modified radical mastectomy.</div></div><div><h3>Method</h3><div>A non-inferiority, randomized controlled study was performed in 100 patients aged 18-60 years who underwent modified radical mastectomy. In the open approach group (n<!--> <!-->=<!--> <!-->50), 30<!--> <!-->ml of 0.25% bupivacaine was injected deep to the serratus anterior muscle after breast resection and rib palpation. In the ultrasound-guided group (n<!--> <!-->=<!--> <!-->50), 30<!--> <!-->ml of 0.25% bupivacaine was injected deep to the serratus anterior muscle under ultrasound guidance before the skin incision. The primary outcome was total morphine consumption in the first 24 postoperative hours. Secondary outcome measures included time to first request for rescue analgesia (duration of SAP block), postoperative visual analogue scale score, and the incidence of adverse effects.</div></div><div><h3>Results</h3><div>Median (interquartile range) morphine requirement in the first 24<!--> <!-->h was similar in both groups (P<!--> <!-->=<!--> <!-->0.81), and the time to first request for analgesia was also similar (P<!--> <!-->=<!--> <!-->0.81). Evaluation of visual analogue scale scores during the initial 24<!--> <!-->h after surgery showed no statistically significant differences between groups (P<!--> <!-->&gt;<!--> <!-->0.05), except at 4 postoperative hours, when the score was significantly higher in the ultrasound group (P<!--> <!-->&lt;<!--> <!-->0.001). No complications were observed in either group.</div></div><div><h3>Conclusion</h3><div>The open approach to deep SAP block is comparable to the ultrasound-guided approach in providing postoperative analgesia in female patients undergoing modified radical mastectomy.</div></div>","PeriodicalId":46479,"journal":{"name":"Revista Espanola de Anestesiologia y Reanimacion","volume":"72 9","pages":"Article 501902"},"PeriodicalIF":0.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145398369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Documento de consenso para la solicitud de pruebas de coagulación preoperatorias 关于要求进行术前凝血试验的协商一致文件
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-11-01 DOI: 10.1016/j.redar.2025.501931
J.L. Jover Pinillos , R. Ferrandis Comes , J.V. Llau Pitarch , D. Zamudio Penko , M. Basora Macaya , M.J. Colomina Soler , A. Abad Gurumeta , J. García Fernández

Introduction

Standard pre-operative coagulation tests have shown limited ability to detect haemostatic abnormalities, yet they continue to be ordered routinely. Clear guidance is lacking in our setting. We therefore sought to develop consensus recommendations on their indication using a Delphi methodology.

Material and methods

A three-round, online, multicentre Delphi study was conducted. Ten expert anaesthesiologists convened and recruited colleagues until the working group comprised 59 anaesthesiologists; 50 completed all rounds. The panel rated 46 statements on a 1-9 Likert scale. Consensus was defined in the third round as ≥ 70% of ratings ≥ 7. Accepted statements were classified as weak (70-79%), moderate (80-89%) or strong (90-100%) agreement.

Results

Twenty-nine statements were accepted, synthesised into 21 and grouped into 6 categories: general indication, patient risk factors, procedure characteristics, global assessment, paediatric patients, and special cases. Agreement was strong for 14 statements, moderate for 5, and weak for 2. Key recommendations were: (1) avoid universal testing; (2) base testing on medical history, standardised bleeding-risk questionnaires and specific risk factors (anticoagulation, liver disease, renal insufficiency, haematopoietic disorders); (3) order tests before high-complexity or high-bleeding-risk procedures; and (4) use specific assays in patients receiving direct oral anticoagulants.

Conclusions

Pre-operative coagulation testing should be individualised according to the patient's haemostatic risk profile and the haemorrhagic risk of the planned procedure.
标准的术前凝血试验显示检测止血异常的能力有限,但仍被常规要求进行。在我们的环境中缺乏明确的指导。因此,我们试图使用德尔菲方法就其适应症提出共识建议。材料与方法进行三轮在线多中心德尔菲研究。10名专家麻醉师召集并招募同事,直至工作组由59名麻醉师组成;50人完成所有回合。该小组以1-9的李克特量表对46个陈述进行了评分。共识在第三轮被定义为≥70%的评分≥7。被接受的陈述被分为弱(70-79%)、中等(80-89%)和强(90-100%)三个级别。结果接受29项陈述,将其综合为21项,分为一般指征、患者危险因素、手术特点、总体评价、儿科患者和特殊病例6类。有14条是强烈赞同,5条是中等赞同,2条是不赞同。主要建议有:(1)避免普遍检测;(2)以病史、标准化的出血危险问卷和特定危险因素(抗凝血、肝病、肾功能不全、造血功能障碍)为基础进行检测;(3)在高复杂性或高出血风险的手术前安排检查;(4)对直接口服抗凝药物的患者进行特异性检测。结论术后凝血试验应根据患者的凝血风险情况和计划手术的出血风险进行个体化。
{"title":"Documento de consenso para la solicitud de pruebas de coagulación preoperatorias","authors":"J.L. Jover Pinillos ,&nbsp;R. Ferrandis Comes ,&nbsp;J.V. Llau Pitarch ,&nbsp;D. Zamudio Penko ,&nbsp;M. Basora Macaya ,&nbsp;M.J. Colomina Soler ,&nbsp;A. Abad Gurumeta ,&nbsp;J. García Fernández","doi":"10.1016/j.redar.2025.501931","DOIUrl":"10.1016/j.redar.2025.501931","url":null,"abstract":"<div><h3>Introduction</h3><div>Standard pre-operative coagulation tests have shown limited ability to detect haemostatic abnormalities, yet they continue to be ordered routinely. Clear guidance is lacking in our setting. We therefore sought to develop consensus recommendations on their indication using a Delphi methodology.</div></div><div><h3>Material and methods</h3><div>A three-round, online, multicentre Delphi study was conducted. Ten expert anaesthesiologists convened and recruited colleagues until the working group comprised 59 anaesthesiologists; 50 completed all rounds. The panel rated 46 statements on a 1-9 Likert scale. Consensus was defined in the third round as ≥<!--> <!-->70% of ratings ≥<!--> <!-->7. Accepted statements were classified as weak (70-79%), moderate (80-89%) or strong (90-100%) agreement.</div></div><div><h3>Results</h3><div>Twenty-nine statements were accepted, synthesised into 21 and grouped into 6 categories: general indication, patient risk factors, procedure characteristics, global assessment, paediatric patients, and special cases. Agreement was strong for 14 statements, moderate for 5, and weak for 2. Key recommendations were: (1)<!--> <!-->avoid universal testing; (2)<!--> <!-->base testing on medical history, standardised bleeding-risk questionnaires and specific risk factors (anticoagulation, liver disease, renal insufficiency, haematopoietic disorders); (3)<!--> <!-->order tests before high-complexity or high-bleeding-risk procedures; and (4)<!--> <!-->use specific assays in patients receiving direct oral anticoagulants.</div></div><div><h3>Conclusions</h3><div>Pre-operative coagulation testing should be individualised according to the patient's haemostatic risk profile and the haemorrhagic risk of the planned procedure.</div></div>","PeriodicalId":46479,"journal":{"name":"Revista Espanola de Anestesiologia y Reanimacion","volume":"72 9","pages":"Article 501931"},"PeriodicalIF":0.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145398323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efectos de la anestesia en la función cognitiva en ratones jóvenes y mayores: rol de la expresión de c-Fos en la excitabilidad neuronal del hipocampo 麻醉对年轻和年老小鼠认知功能的影响:c-Fos表达在海马体神经兴奋性中的作用
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-11-01 DOI: 10.1016/j.redar.2025.501904
Y. Li , Z. Jin , L. Beiping , A. Xiaolei

Objective

This study aims to investigate the effects of anaesthesia and surgical procedures on the cognitive function of both young and aged mice. It will also explore the role and mechanisms of c-Fos expression in altering hippocampal neuron excitability and its relationship with perioperative neurocognitive disorders in mice.

Methods

In this study, we used a murine laparotomy model to assess cognitive behavioural changes in both young and aged mice at 1, 3, and 7 days post-surgery. We used immunofluorescence techniques to evaluate c-Fos expression in the dorsal hippocampus of aged mice following laparotomy. We also used a chemical genetic approach, injecting a virus into the dorsal hippocampus to modulate neuronal excitability, and subsequently analysed changes in object location memory (OLM) and temporal order memory (TOM). Furthermore, we used Golgi staining to observe the density of dendritic spines in the dorsal hippocampal region of aged mice following anaesthesia and viral injection.

Results

No significant cognitive differences were observed between young mice and aged mice administered anaesthesia alone, compared to their respective control groups. However, only the aged mice that underwent surgery displayed significant deficits in OLM and T-maze (TOM) tasks one day post-surgery (one-way ANOVA: OLM: F = 27.507, P < .001; TOM: F = 12.196, P < .001), while the OLM recognition index showed no significant change (one-way ANOVA: F = .057, P = .982). Furthermore, c-Fos positive neurons in the dorsal hippocampus of aged mice decreased significantly one day after surgery (one-way ANOVA: F = .057, P = .0048). The application of a chemical-genetic method to enhance the excitability of neurons in the dorsal hippocampus effectively reversed surgery-induced cognitive impairment (one-way ANOVA: OLM: F = .032, P = .021; TOM: F = .024, P = .019) and depletion of dendritic spine density (P < .01).

Conclusion

Temporal and spatial memory functions are impaired in aged mice following surgery, while object recognition memory remains unaffected. Surgical procedures result in a decrease in the number of c-Fos positive neurons and neuronal excitability in the dorsal hippocampus of aged mice. Furthermore, enhancing neuronal excitability in the dorsal hippocampus alleviates postoperative cognitive impairment in aged mice. Additionally, increasing neuronal excitability in the dorsal hippocampus can counteract the surgery-induced reduction in dendritic spine density.
目的探讨麻醉和手术对幼龄和老年小鼠认知功能的影响。探讨c-Fos表达在改变小鼠海马神经元兴奋性中的作用和机制及其与围手术期神经认知障碍的关系。方法在本研究中,我们使用小鼠剖腹手术模型来评估术后1、3和7天幼龄和老年小鼠的认知行为变化。我们采用免疫荧光技术检测老年小鼠剖腹手术后海马背侧c-Fos的表达。我们还使用了化学遗传方法,向海马背侧注射病毒来调节神经元的兴奋性,随后分析了物体位置记忆(OLM)和时间顺序记忆(TOM)的变化。此外,我们用高尔基染色法观察麻醉和注射病毒后老年小鼠海马背区树突棘的密度。结果与各自的对照组相比,单独麻醉的年轻小鼠和老年小鼠之间没有明显的认知差异。然而,只有接受手术的老年小鼠在术后1天出现OLM和T-maze (TOM)任务的显著缺陷(单因素方差分析:OLM: F = 27.507, P < .001; TOM: F = 12.196, P < .001),而OLM识别指数无显著变化(单因素方差分析:F = 0.057, P = .982)。老年小鼠术后1天海马背侧c-Fos阳性神经元显著减少(单因素方差分析:F = 0.057, P = 0.0048)。应用化学遗传学方法增强海马背侧神经元的兴奋性可有效逆转手术引起的认知障碍(单因素方差分析:OLM: F = 0.032, P = 0.021; TOM: F = 0.024, P = 0.019)和树突棘密度的消耗(P < 0.01)。结论老年小鼠手术后时间和空间记忆功能受损,而物体识别记忆未受影响。手术导致老年小鼠海马背侧c-Fos阳性神经元数量减少,神经元兴奋性降低。此外,增强海马背侧神经元兴奋性可减轻老年小鼠术后认知功能障碍。此外,海马背侧神经元兴奋性的增加可以抵消手术引起的树突棘密度的减少。
{"title":"Efectos de la anestesia en la función cognitiva en ratones jóvenes y mayores: rol de la expresión de c-Fos en la excitabilidad neuronal del hipocampo","authors":"Y. Li ,&nbsp;Z. Jin ,&nbsp;L. Beiping ,&nbsp;A. Xiaolei","doi":"10.1016/j.redar.2025.501904","DOIUrl":"10.1016/j.redar.2025.501904","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to investigate the effects of anaesthesia and surgical procedures on the cognitive function of both young and aged mice. It will also explore the role and mechanisms of c-Fos expression in altering hippocampal neuron excitability and its relationship with perioperative neurocognitive disorders in mice.</div></div><div><h3>Methods</h3><div>In this study, we used a murine laparotomy model to assess cognitive behavioural changes in both young and aged mice at 1, 3, and 7<!--> <!-->days post-surgery. We used immunofluorescence techniques to evaluate c-Fos expression in the dorsal hippocampus of aged mice following laparotomy. We also used a chemical genetic approach, injecting a virus into the dorsal hippocampus to modulate neuronal excitability, and subsequently analysed changes in object location memory (OLM) and temporal order memory (TOM). Furthermore, we used Golgi staining to observe the density of dendritic spines in the dorsal hippocampal region of aged mice following anaesthesia and viral injection.</div></div><div><h3>Results</h3><div>No significant cognitive differences were observed between young mice and aged mice administered anaesthesia alone, compared to their respective control groups. However, only the aged mice that underwent surgery displayed significant deficits in OLM and T-maze (TOM) tasks one day post-surgery (one-way ANOVA: OLM: F<!--> <!-->=<!--> <!-->27.507, <em>P</em> <!-->&lt;<!--> <!-->.001; TOM: F<!--> <!-->=<!--> <!-->12.196, <em>P</em> <!-->&lt;<!--> <!-->.001), while the OLM recognition index showed no significant change (one-way ANOVA: F<!--> <!-->=<!--> <!-->.057, <em>P</em> <!-->=<!--> <!-->.982). Furthermore, c-Fos positive neurons in the dorsal hippocampus of aged mice decreased significantly one day after surgery (one-way ANOVA: F<!--> <!-->=<!--> <!-->.057, <em>P</em> <!-->=<!--> <!-->.0048). The application of a chemical-genetic method to enhance the excitability of neurons in the dorsal hippocampus effectively reversed surgery-induced cognitive impairment (one-way ANOVA: OLM: F<!--> <!-->=<!--> <!-->.032, <em>P</em> <!-->=<!--> <!-->.021; TOM: F<!--> <!-->=<!--> <!-->.024, <em>P</em> <!-->=<!--> <!-->.019) and depletion of dendritic spine density (<em>P</em> <!-->&lt;<!--> <!-->.01).</div></div><div><h3>Conclusion</h3><div>Temporal and spatial memory functions are impaired in aged mice following surgery, while object recognition memory remains unaffected. Surgical procedures result in a decrease in the number of c-Fos positive neurons and neuronal excitability in the dorsal hippocampus of aged mice. Furthermore, enhancing neuronal excitability in the dorsal hippocampus alleviates postoperative cognitive impairment in aged mice. Additionally, increasing neuronal excitability in the dorsal hippocampus can counteract the surgery-induced reduction in dendritic spine density.</div></div>","PeriodicalId":46479,"journal":{"name":"Revista Espanola de Anestesiologia y Reanimacion","volume":"72 9","pages":"Article 501904"},"PeriodicalIF":0.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145398372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Delirium postoperatorio: de la evidencia internacional a la práctica en España. Una asignatura pendiente 术后精神错乱:从国际证据到西班牙的实践。一个悬而未决的问题
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-07-23 DOI: 10.1016/j.redar.2025.501920
E.M. Aldana , C. Aldecoa
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引用次数: 0
Índices Doppler de la arteria cubital, un nuevo predictor emergente de la preservación segmentaria del cúbito después del bloqueo supraclavicular: un estudio observacional prospectivo 宫颈动脉多普勒指数:颞上阻塞后宫颈段保存的新预测指标:一项前瞻性观察研究
IF 0.8 Q3 ANESTHESIOLOGY Pub Date : 2025-07-21 DOI: 10.1016/j.redar.2025.501899
M. Adolf Helmy, M. Medhat Megalaa, H. Mohamed Mamdouh, I.A. Ismail, L. Magdy Milad

Background

Supraclavicular brachial plexus block offers several advantages over general anesthesia, yet segmental ulnar sparing is one of the procedure's key drawbacks. Many parameters were proposed to ascertain segmental ulnar sparing, including thermal imaging and perfusion index. However, none proved optimal; therefore, we aimed to evaluate ulnar artery Doppler indices as a potential predictor of segmental ulnar sparing.

Methods

This prospective observational study was conducted at a tertiary center. To obtain a study power of 90% and an alpha error of 0.05, a minimum sample size of 62 patients was required, with a minimum of 11 patients exhibiting segmental ulnar sparing. Adult patients who received supraclavicular brachial plexus block for hand surgery were included. Each dermatome was assessed in terms of motor and sensory function. An experienced operator performed Doppler examinations at baseline, 10-, 20-, and 30 minutes following block administration. The primary outcome was the accuracy of the change in PI (ΔPI) in predicting segmental ulnar sparing. Other outcomes included the change in resistive index (ΔRI) and the change in waveform morphology (ΔM) to predict segmental ulnar sparing

Results

Of the 81 patients assessed for eligibility, 15 were excluded, leaving sixty-six in the final analysis. Eleven of the 66 individuals showed segmental ulnar sparing. ΔPI and ΔRI have been identified as accurate predictors of segmental ulnar sparing with best cut-off values of < 8.8% and 12%, respectively. Additionally, ulnar artery Doppler morphological changes from triphasic pattern to a monophasic wave can rule out segmental ulnar sparing with a 100% negative predictive value.

Conclusion

In adult patients undergoing hand surgery using supraclavicular brachial plexus block, Changes of Doppler indices, namely ΔPI, ΔRI, and ΔM, showed good accuracy in predicting segmental ulna preservation. However, the generalizability of our findings is limited by being a single-center study with a relatively small sample size.
背景锁骨上臂丛阻滞与全身麻醉相比有许多优点,但节段性尺骨保留是该手术的主要缺点之一。提出了许多参数来确定节段性尺骨保留,包括热成像和灌注指数。然而,没有一个被证明是最佳的;因此,我们旨在评估尺动脉多普勒指数作为节段性尺动脉保留的潜在预测指标。方法本前瞻性观察研究在某三级医学中心进行。为了获得90%的研究效能和0.05的α误差,至少需要62例患者的样本量,其中至少有11例患者表现出节段性尺骨保留。在手外科手术中接受锁骨上臂丛阻滞的成年患者也包括在内。根据运动和感觉功能评估每个皮节。一位经验丰富的操作员在阻滞给药后的基线、10分钟、20分钟和30分钟进行多普勒检查。主要结果是PI变化预测尺节段性保留的准确性(ΔPI)。其他结果包括电阻指数的变化(ΔRI)和波形形态的变化(ΔM),以预测节段性尺节段性保留。结果在81例评估合格的患者中,15例被排除,剩下66例最终分析。66例患者中有11例尺节段性保留。ΔPI和ΔRI已被确定为节段性尺骨保留的准确预测因子,最佳临界值分别为8.8%和12%。此外,尺动脉多普勒形态学变化从三相模式到单相波可以排除节段性尺动脉保留,100%阴性预测值。结论在锁骨上臂丛神经阻滞行手外科手术的成年患者中,多普勒指数ΔPI、ΔRI和ΔM的变化对预测尺骨节段保留有较好的准确性。然而,我们的研究结果的普遍性受到单中心研究和相对较小的样本量的限制。
{"title":"Índices Doppler de la arteria cubital, un nuevo predictor emergente de la preservación segmentaria del cúbito después del bloqueo supraclavicular: un estudio observacional prospectivo","authors":"M. Adolf Helmy,&nbsp;M. Medhat Megalaa,&nbsp;H. Mohamed Mamdouh,&nbsp;I.A. Ismail,&nbsp;L. Magdy Milad","doi":"10.1016/j.redar.2025.501899","DOIUrl":"10.1016/j.redar.2025.501899","url":null,"abstract":"<div><h3>Background</h3><div>Supraclavicular brachial plexus block offers several advantages over general anesthesia, yet segmental ulnar sparing is one of the procedure's key drawbacks. Many parameters were proposed to ascertain segmental ulnar sparing, including thermal imaging and perfusion index. However, none proved optimal; therefore, we aimed to evaluate ulnar artery Doppler indices as a potential predictor of segmental ulnar sparing.</div></div><div><h3>Methods</h3><div>This prospective observational study was conducted at a tertiary center. To obtain a study power of 90% and an alpha error of 0.05, a minimum sample size of 62 patients was required, with a minimum of 11 patients exhibiting segmental ulnar sparing. Adult patients who received supraclavicular brachial plexus block for hand surgery were included. Each dermatome was assessed in terms of motor and sensory function. An experienced operator performed Doppler examinations at baseline, 10-, 20-, and 30<!--> <!-->minutes following block administration. The primary outcome was the accuracy of the change in PI (ΔPI) in predicting segmental ulnar sparing. Other outcomes included the change in resistive index (ΔRI) and the change in waveform morphology (ΔM) to predict segmental ulnar sparing</div></div><div><h3>Results</h3><div>Of the 81 patients assessed for eligibility, 15 were excluded, leaving sixty-six in the final analysis. Eleven of the 66 individuals showed segmental ulnar sparing. ΔPI and ΔRI have been identified as accurate predictors of segmental ulnar sparing with best cut-off values of &lt;<!--> <!-->8.8% and 12%, respectively. Additionally, ulnar artery Doppler morphological changes from triphasic pattern to a monophasic wave can rule out segmental ulnar sparing with a 100% negative predictive value.</div></div><div><h3>Conclusion</h3><div>In adult patients undergoing hand surgery using supraclavicular brachial plexus block, Changes of Doppler indices, namely ΔPI, ΔRI, and ΔM, showed good accuracy in predicting segmental ulna preservation. However, the generalizability of our findings is limited by being a single-center study with a relatively small sample size.</div></div>","PeriodicalId":46479,"journal":{"name":"Revista Espanola de Anestesiologia y Reanimacion","volume":"72 8","pages":"Article 501899"},"PeriodicalIF":0.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Revista Espanola de Anestesiologia y Reanimacion
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