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USP7 Stabilizes MESP1 To Promote the Malignant Progression of Non-Small Cell Lung Cancer. USP7稳定MESP1促进非小细胞肺癌的恶性进展
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-21 DOI: 10.1007/s12010-025-05510-y
Shasha Jiang, Liwen Rong, Fei Yi, Peng Yang, Longjing Yang

Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related deaths worldwide, necessitating the identification of novel therapeutic targets. Mesoderm posterior bHLH transcription factor 1 (MESP1) has been implicated in various developmental processes, but its role in cancer, particularly NSCLC, is poorly understood. Given the emerging evidence linking MESP1 to cellular processes relevant to cancer biology, investigating its regulatory mechanisms in NSCLC could provide critical insights for developing new therapies. This study employed quantitative real-time PCR (qRT-PCR) to assess the mRNA levels of MESP1, ubiquitin specific peptidase 7 (USP7), and clusters of differentiation 163 (CD163). Western blotting was used to analyze the protein expression of MESP1 and USP7. Cellular proliferation was evaluated through colony-forming assays, while apoptosis was quantified using flow cytometry. Mitochondrial membrane potential was measured by JC-1 staining, and reactive oxygen species (ROS) levels were also analyzed via flow cytometry. Additionally, colorimetric assays were utilized to determine malondialdehyde (MDA), total iron, and Fe2+ levels. The in vivo effects of MESP1 silencing on NSCLC progression were examined using a xenograft mouse model. GST-pull down assay, Co-immunoprecipitation (Co-IP) assay, and ubiquitination assay were conducted to explore the interaction between USP7 and MESP1. The expression of both MESP1 and USP7 was found to be upregulated in NSCLC tissues and cells when compared with normal lung tissues and normal human bronchial epithelial cells. Knockdown of MESP1 significantly inhibited NSCLC cell proliferation, induced apoptosis and promoted features associated with ferroptosis. Moreover, MESP1 silencing suppressed M2 macrophage polarization and tumor formation. Mechanistically, USP7 was identified to stabilize MESP1 protein expression through its deubiquitinating activity. Overexpression of MESP1 attenuated the inhibitory effects of USP7 silencing on NSCLC cell proliferation and M2 macrophage polarization and also mitigated the promoting effects of USP7 knockdown on apoptosis and the induction of features associated with ferroptosis. USP7 stabilized MESP1 to promote the malignant progression of NSCLC. The findings highlight the potential of targeting the USP7-MESP1 axis as a novel therapeutic strategy for NSCLC.

非小细胞肺癌(NSCLC)仍然是世界范围内癌症相关死亡的主要原因,需要确定新的治疗靶点。中胚层后bHLH转录因子1 (MESP1)参与多种发育过程,但其在癌症,特别是非小细胞肺癌中的作用尚不清楚。鉴于将MESP1与癌症生物学相关的细胞过程联系起来的新证据,研究其在非小细胞肺癌中的调节机制可以为开发新疗法提供重要见解。本研究采用实时荧光定量PCR (qRT-PCR)检测MESP1、泛素特异性肽酶7 (USP7)和分化簇163 (CD163) mRNA水平。Western blotting检测MESP1和USP7蛋白表达情况。通过集落形成试验评估细胞增殖,而流式细胞术量化细胞凋亡。JC-1染色检测线粒体膜电位,流式细胞术检测活性氧(ROS)水平。此外,采用比色法测定丙二醛(MDA)、总铁和Fe2+水平。使用异种移植小鼠模型研究了MESP1沉默对NSCLC进展的体内影响。采用GST-pull down法、Co-IP法和泛素化法研究USP7与MESP1的相互作用。与正常肺组织和正常人支气管上皮细胞相比,MESP1和USP7在NSCLC组织和细胞中的表达均上调。MESP1敲低可显著抑制NSCLC细胞增殖,诱导细胞凋亡,促进铁下垂相关特征。此外,MESP1沉默抑制M2巨噬细胞极化和肿瘤形成。机制上,USP7通过其去泛素化活性稳定MESP1蛋白的表达。MESP1过表达减弱了USP7沉默对NSCLC细胞增殖和M2巨噬细胞极化的抑制作用,也减弱了USP7敲低对细胞凋亡的促进作用和对铁凋亡相关特征的诱导作用。USP7稳定MESP1促进NSCLC的恶性进展。这些发现强调了靶向USP7-MESP1轴作为非小细胞肺癌新治疗策略的潜力。
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引用次数: 0
Exploration of Mechanisms of Keliu Pill on the Inhibition of Lymphangiogenesis in Non-small Cell Lung Cancer Based on Network Pharmacology and Experimental Validation. 基于网络药理学及实验验证的克流丸抑制非小细胞肺癌淋巴管生成机制探讨。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-21 DOI: 10.1007/s12010-025-05307-z
Shuang Yang, Xiao Wu, Tingyu Pan, Weizhou Zhang, Wenpan Peng, Zhichao Wang, Fanchao Feng, Yong Xu, Ying Yang, Hailang He, Xianmei Zhou

Keliu Pill (KLW) is a Chinese medicine formula that has been shown to be effective in treating non-small cell lung cancer (NSCLC) patients. However, the potential molecular mechanism remains unclear. To explore the underlying mechanism of Keliu Pill (KLW) in treating NSCLC, the network pharmacology was applied to explore the potential mechanism of KLW in the treatment of lung cancer. With oral bioavailability (OB) ≥ 30% and drug-like index (DL) ≥ 0.18 as the filter criteria, the compounds of 6 traditional Chinese medicines (TCMs) from KLW were retrieved on Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform (TCMSP) and supplemented by Traditional Chinese Medicine Comprehensive Database (TCMID). The potential targets corresponding to the active components of the TCMs mentioned above were obtained from TCMSP. Subsequently, the potential underlying action mechanisms of KLW on NSCLC predicted by the network pharmacology analyses were experimentally validated in Lewis lung cancer cell inoculation mice models and co-culture models with Lewis lung cancer cell and tumor-associated macrophages (TAMs). After database search and screening, a total of 20 active ingredients of Astragalus Membranaceus, 21 of Sophare Tonkinensis Radix et Rhizoma, 1 of Mylabris, 3 of Eupolyphaga, 1 of Leech, and 3 of Gecko were obtained. After deleting duplicates, 49 active ingredients were obtained. Thirty important active ingredients in KLW were sorted out by referring to relevant literature. The KEGG pathway enrichment analysis based on the DAVID platform showed that the VEGF-C signaling pathway may be the core signaling pathway associated with KLW in the treatment of NSCLC. The network pharmacological analysis demonstrated that the various components of KLW acted on NSCLC through 29 potential targets such as VEGF-C, VEGF-D, MAPK8, MAPK1, and AKT1. The experiments in vivo indicated KLW could inhibit the transcription and translation of VEGF-C and VEGF-D in tumor tissues. KLW could reduce the proportion of activated TAMs in the tissue. KLW-contained serum may decrease the expression level of VEGF-C and VEGF-D and inhibit the lymphatic lumen formation of human lymphatic endothelial cells. VEGFC and VEGFD were confirmed as the potential KLW-associated targets for NSCLC. KLW may inhibit lymphatic angiogenesis of lung cancer by regulating the function of TAMs.

克流丸(KLW)是一种中药配方,已被证明对治疗非小细胞肺癌(NSCLC)患者有效。然而,潜在的分子机制尚不清楚。为探讨克利流丸(KLW)治疗非小细胞肺癌的潜在机制,应用网络药理学方法探讨KLW治疗肺癌的潜在机制。以口服生物利用度(OB)≥30%,类药指数(DL)≥0.18为筛选标准,从中药系统药理学数据库和分析平台(TCMSP)中检索到KLW中6种中药的化合物,并辅以中药综合数据库(TCMID)。上述中药有效成分对应的潜在靶点均从中药复方中得到。随后,通过Lewis肺癌细胞接种小鼠模型和Lewis肺癌细胞与肿瘤相关巨噬细胞(tam)共培养模型,实验验证了网络药理学分析预测的KLW对NSCLC的潜在作用机制。经数据库检索和筛选,共获得黄芪20种有效成分,冬参21种,大耳草1种,巨食草3种,水蛭1种,壁虎3种。删除重复项后,得到49种有效成分。通过查阅相关文献,整理出KLW中30种重要的有效成分。基于DAVID平台的KEGG通路富集分析显示VEGF-C信号通路可能是与KLW治疗NSCLC相关的核心信号通路。网络药理学分析表明,KLW的各种成分通过VEGF-C、VEGF-D、MAPK8、MAPK1和AKT1等29个潜在靶点作用于NSCLC。体内实验表明,KLW可抑制肿瘤组织中VEGF-C和VEGF-D的转录和翻译。KLW可降低组织中活化tam的比例。含klw的血清可降低人淋巴内皮细胞VEGF-C和VEGF-D的表达水平,抑制淋巴管腔的形成。VEGFC和VEGFD被证实为NSCLC的潜在klw相关靶点。KLW可能通过调节tam的功能抑制肺癌淋巴血管生成。
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引用次数: 0
Investigating the Causes, Control Strategies, Challenges, and Future Perspectives of Membrane Biofouling in Quorum-sensing Membrane Bioreactors. 群体感应膜生物反应器中膜生物污染的原因、控制策略、挑战和未来展望。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-21 DOI: 10.1007/s12010-025-05570-0
Shuli Liu, Wenxiao Wang, Yuhong Zhang, Yatong Gao, Xiaohong Han, Zhihui Kong, Haoyi Guo, Qi Li, Ning Guo, Jia Kang, Zhixin Song, Zhaoyong Ye, Gangfu Song

In recent years, bacterial quorum quenching (QQ) has emerged as an effective strategy for reducing biofouling in membrane bioreactors (MBRs). Understanding microbial community dynamics is crucial for developing effective QQ strategies, as changes in these communities can significantly influence the risk of biofouling in the sludge. This study systematically investigates the formation, mechanisms, and regulatory strategies related to biofouling in MBRs. It offers a comprehensive analysis of quorum sensing (QS) mechanisms within microbial communities and their biofouling tendencies. Moreover, the interactions between quorum sensing, extracellular polymerization, and membrane biofouling are discussed. Additionally, the short-term addition of exogenous QQ was found to temporarily cause a reduction in the sludge's QQ capabilities, thereby increasing its susceptibility to membrane biofouling. The study concludes with future perspectives on managing biofouling in membrane bioreactors and provides recommendations for further research on leveraging QS-MBR systems to mitigate membrane biofouling.

近年来,细菌群体猝灭(QQ)已成为膜生物反应器(mbr)中减少生物污染的一种有效策略。了解微生物群落动态对于制定有效的QQ策略至关重要,因为这些群落的变化会显著影响污泥中生物污染的风险。本研究系统地探讨了mbr中生物污染的形成、机制和调控策略。它提供了微生物群落中群体感应(QS)机制及其生物污染倾向的综合分析。此外,还讨论了群体感应、细胞外聚合和膜生物污染之间的相互作用。此外,短期添加外源QQ会暂时降低污泥的QQ能力,从而增加其对膜生物污染的易感性。最后,对膜生物反应器中生物污染的治理进行了展望,并对利用QS-MBR系统缓解膜生物污染的进一步研究提出了建议。
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引用次数: 0
Effects of (5R)-5-Hydroxytriptolide Against Rheumatoid Arthritis: Intervention on the Transformation of Bone Marrow Cells Induced Osteoclasts Bone Erosion and Angiogenesis. (5R)-5-羟基雷公藤甲素对类风湿关节炎的作用:干预骨髓细胞转化诱导的破骨细胞、骨侵蚀和血管生成。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s12010-026-05584-2
Ya Yan, Hong Nie, Yanling Lian, Yi Shen, Qin Ding
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引用次数: 0
Identification of Diosmetin, Arbutin, and Phenyl Glucoside as Novel Inhibitors from Origanum majorana Targeting Human Cyclooxygenase-2 Enzyme: Insight from Virtual Screening, MD Simulation and Density Functional Theory. 牛头草中黄叶皂苷、熊果苷和苯基葡萄糖苷作为针对人环氧合酶-2的新型抑制剂的鉴定:来自虚拟筛选、MD模拟和密度泛函数理论的见解。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s12010-026-05581-5
Shilpi Rawat, Priyanka Joshi, Pankaja Pandey, Vijay Arya, Subhash Chandra
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引用次数: 0
Discovery of a Novel Small-Molecule Modulator for Full-length YB-1 Protein via Integrated Computational and in vitro Biophysical Approaches. 利用综合计算和体外生物物理方法发现一种新的全长YB-1蛋白小分子调节剂。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s12010-025-05492-x
Maharaja Somasundaram, Pandaram Sakthivel, Sakthi Sasikala Sundaravel, Sneha Jos, Karthikeyan Muthusamy, Sivaraman Thirunavukkarasu, Ilangovan Andivelu, Mathan Ganeshan
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引用次数: 0
Combinatorial Study of Deep Eutectic Solvent and Ultrasonication Pretreatment in Napier Grass to Enhance Bioethanol Production. 深共熔溶剂与超声预处理相结合提高纳皮草生物乙醇产量的研究。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-19 DOI: 10.1007/s12010-025-05566-w
Diana Jose, Rosshini Sivakumar, Sukunya Areeya, Muthu Kumar Sampath, Hassan El Bari, Wanwitoo Wanmolee, Santi Chuetor, Ponnusami Venkatachalam, Malinee Sriariyanun
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引用次数: 0
Cresol Derivatives from Bacillus subtilis as Natural Oviposition Modulator of Culex quinquefasciatus: A Molecular Docking Approach. 枯草芽孢杆菌甲酚衍生物作为致倦库蚊天然产卵调节剂的分子对接研究。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-19 DOI: 10.1007/s12010-025-05563-z
Basanta Sarkar, Souvik Bag, Abhijit Mandal, Dibyendu Saha, Shubhajit Saha, Rama Bhaduri, Soumendranath Chatterjee

Mosquitoes rely heavily on olfactory cues for locating suitable oviposition sites, with microbial communities in aquatic habitats playing a crucial role in producing volatile organic compounds (VOCs) that influence mosquito behaviour. In this study, we isolated Bacillus subtilis DHB13 from the breeding habitat of Culex quinquefasciatus, a major vector of several human diseases. The partial 16S rRNA gene sequence of the isolate has been submitted to NCBI GenBank with the accession number PV698100. The identity and resistance profile of the strain was confirmed through biochemical and antibiotic susceptibility tests. The bacterial suspension demonstrated a notable oviposition activity index (OAI) of 0.77 ± SE, with moderate variation among treatments (F(3, 8) = 3.631, p = 0.0642). Multiple comparison analysis (Tukey's test) showed that OAI values for DHB13-treated media did not differ significantly from natural habitat water but were significantly higher than the sterile control, indicating a biologically relevant attraction of gravid female mosquitoes. LC-MS analysis of the bacterial culture supernatant revealed the presence of three cresol derivatives: diisopropyl-m-cresol, 3-ethyl-p-cresol, and 6-ethyl-o-cresol. These compounds were evaluated through molecular docking against Cx. quinquefasciatus Odorant Binding Protein 1 (CxOBP1), a protein known to mediate olfactory-driven oviposition behaviour. However, mosquito olfaction involves several OBPs, receptors, and enzymes, so interaction with CxOBP1 represents only part of this complex sensory system. Molecular docking revealed strong binding of CxOBP1 with diisopropyl-m-cresol (-6.7 kcal/mol), 3-ethyl-p-cresol (-6.2 kcal/mol), and 6-ethyl-o-cresol (-5.9 kcal/mol), indicating potential oviposition attractant activity. All three ligands were found to bind within a conserved binding pocket of CxOBP1, behavioural assays confirmed the oviposition-stimulant properties of the bacterial suspension, indicating that the detected compounds mimic natural semio-chemicals such as p-cresol, previously recognized as an oviposition cue. These findings reinforce the role of microbiota in shaping mosquito reproductive behaviour through the production of volatile attractants. Moreover, they highlight the potential of using microbial VOCs as environmentally sustainable tools for mosquito surveillance and vector control. This integrative approach linking microbial ecology, chemical analysis, and mosquito behaviour provides novel insights for the development of attractant-based control strategies.

蚊子在很大程度上依赖嗅觉线索来寻找合适的产卵地点,水生栖息地的微生物群落在产生影响蚊子行为的挥发性有机化合物(VOCs)方面发挥着至关重要的作用。本研究从致倦库蚊孳生地分离出枯草芽孢杆菌DHB13,致倦库蚊是几种人类疾病的主要媒介。分离物的部分16S rRNA基因序列已提交至NCBI GenBank,登录号为PV698100。通过生化试验和药敏试验确定菌株的鉴定和耐药谱。细菌悬浮液产卵活性指数(OAI)为0.77±SE,各处理间差异不大(F(3,8) = 3.631, p = 0.0642)。多重比较分析(Tukey’s test)显示,处理dhb13培养基的OAI值与自然生境水体差异不显著,但显著高于无菌对照,表明存在孕雌蚊的生物学相关吸引。细菌培养上清的LC-MS分析显示存在三种甲酚衍生物:二异丙基间甲酚、3-乙基对甲酚和6-乙基邻甲酚。这些化合物通过与Cx的分子对接进行了评价。致倦库蚊气味结合蛋白1 (CxOBP1),一种已知介导嗅觉驱动的产卵行为的蛋白。然而,蚊子的嗅觉涉及多种obp、受体和酶,因此与CxOBP1的相互作用只代表了这个复杂感觉系统的一部分。分子对接显示,CxOBP1与二异丙基间甲酚(-6.7 kcal/mol)、3-乙基对甲酚(-6.2 kcal/mol)和6-乙基邻甲酚(-5.9 kcal/mol)有较强的结合,表明其具有潜在的诱卵活性。所有三种配体都被发现结合在CxOBP1的一个保守结合口袋内,行为分析证实了细菌悬浮液的促排卵特性,表明检测到的化合物模拟了天然半化学物质,如对甲酚,以前被认为是产卵线索。这些发现强化了微生物群通过产生挥发性引诱剂来塑造蚊子繁殖行为的作用。此外,它们强调了利用微生物挥发性有机化合物作为环境可持续的蚊虫监测和媒介控制工具的潜力。这种将微生物生态学、化学分析和蚊子行为联系起来的综合方法为基于引诱剂的控制策略的发展提供了新的见解。
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引用次数: 0
A Plasma Membrane-Located Lysophosphatidic Acid Acyltransferase in Microalga Myrmecia incisa Prefers Arachidonic Acid-CoA to Produce Glycerolipids. 一种位于质膜上的溶血磷脂酸酰基转移酶倾向于花生四烯酸辅酶A产生甘油脂。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-16 DOI: 10.1007/s12010-025-05574-w
Yi-Na Chang, Jiang-Min Yang, Hong Bao, Derek M Ayittey, Zheng Sun, Zhi-Gang Zhou

Lysophosphatidic acid acyltransferase (LPAAT) is a pivotal enzyme in the de novo biosynthesis of phosphatidic acid (PA), playing a central role in glycerophospholipid assembly and triacylglycerol (TAG) accumulation. Myrmecia incisa is a green microalga notable for its high content of arachidonic acid (ArA), yet the molecular mechanism underlying ArA enrichment in TAG remains unclear. In this study, a putative LPAAT gene from M. incisa, designated MiLPAAT, was identified and cloned, followed by systematic structural and functional characterization. Sequence analysis revealed that MiLPAAT contains a conserved PlsC domain and the characteristic H(X)₄D and EGTR motifs. Bioinformatic predictions identified at least one transmembrane domain at the N-terminus, supporting its identity as an integral membrane protein. This was further confirmed by membrane fractionation and Western blot analysis, which demonstrated its association with the membrane fraction. Phylogenetic analysis further demonstrated its close evolutionary relationship to LPAAT homologs in other green algae. Heterologous expression in Escherichia coli, coupled with in vitro enzymatic assays, confirmed that the recombinant MiLPAAT protein possesses LPAAT activity, catalyzing the acylation of LPA with various acyl-CoAs. Among the substrates tested, MiLPAAT exhibited the highest catalytic efficiency toward ArA-CoA (104.8 ± 3.2 nmol/mg/min), followed by oleoyl-CoA (81.5 ± 2.7 nmol/mg/min) and palmitoyl-CoA (68.4 ± 2.1 nmol/mg/min), consistent with the ArA-rich TAG composition observed in M. incisa. Immunogold labeling and immunohistochemical localization experiments revealed that MiLPAAT is predominantly localized at the plasma membrane. Findings of the present study suggest that MiLPAAT plays a critical role in PA biosynthesis and assembly of ArA into TAG in M. incisa, providing a novel target for microalgal lipid metabolic engineering.

溶血磷脂酸酰基转移酶(LPAAT)是磷脂酸(PA)新生生物合成中的关键酶,在甘油磷脂组装和三酰甘油(TAG)积累中起核心作用。桃金草(Myrmecia incisa)是一种以花生四烯酸(ArA)含量高而闻名的绿色微藻,但ArA在TAG中富集的分子机制尚不清楚。本研究鉴定并克隆了一种LPAAT基因MiLPAAT,并对其进行了系统的结构和功能表征。序列分析表明,MiLPAAT含有一个保守的PlsC结构域和特征的H(X)₄D和EGTR基序。生物信息学预测在n端发现了至少一个跨膜结构域,支持其作为完整膜蛋白的身份。膜分离和Western blot分析进一步证实了这一点,表明其与膜分离有关。系统发育分析进一步证实了其与其他绿藻中LPAAT同源物的密切进化关系。在大肠杆菌中的异源表达,结合体外酶促实验,证实重组MiLPAAT蛋白具有LPAAT活性,可催化LPA与各种酰基辅酶a的酰化。在所测试的底物中,MiLPAAT对ArA-CoA的催化效率最高(104.8±3.2 nmol/mg/min),其次是油酰coa(81.5±2.7 nmol/mg/min)和棕榈酰coa(68.4±2.1 nmol/mg/min),这与M. incisa中观察到的富含ara的TAG组成一致。免疫金标记和免疫组织化学定位实验显示,MiLPAAT主要定位于质膜。本研究结果表明,MiLPAAT在M. incisa的PA生物合成和ArA组装成TAG中起着关键作用,为微藻脂质代谢工程提供了新的靶点。
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引用次数: 0
Investigation of the In Vitro Neuroprotective Potential of Aegiceras corniculatum against MPTP-induced Toxicity. 圆叶蝉体外抗mptp毒性神经保护作用的研究。
IF 3.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-16 DOI: 10.1007/s12010-025-05486-9
Nitu Kumari, Vaddi Damodara Reddy, Santosh Anand

Aegiceras corniculatum (AC), a mangrove species widely recognized for its diverse pharmacological properties, has attracted significant attention recently due to its antioxidant, anti-inflammatory, and antimicrobial activities. Despite this, the neuroprotective potential of AC, particularly in the context of neurodegenerative disorders such as Parkinson's disease (PD), remains largely unexplored. Due to the growing interest in plant-derived compounds for the management of PD, investigating the therapeutic relevance of AC could provide new insights into alternative strategies for neuroprotection. The present study aimed to evaluate the neuroprotective ability of the leaf extract of AC against PD. Preliminary phytochemical analyses were conducted to identify the presence of bioactive compounds in the AC extract (ACE). The antioxidant capacity of ACE was evaluated using three standard assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferric reducing antioxidant power (FRAP) assay, and hydrogen peroxide (H₂O₂) scavenging assay. We used the MPTP-induced PD model established in an SH-SY5Y human neuroblastoma cell line as well as in the U87-MG glioblastoma cell line. MTT assay was conducted to measure the cell viability, and further, 2',7'-dichlorofluorescin diacetate (DCFDA) assay was used to measure the reactive oxygen species (ROS). Glutathione peroxidase (GPx) enzyme activity was determined by a chromogenic reaction-based assay. Western blot analysis was used to assess the expression levels of SNCA protein. The ACE was found to contain a diverse range of phytochemicals, including polyphenols, flavonoids, and terpenoids. Among the ethanolic, hydroethanolic, and aqueous extracts evaluated, ethanolic ACE (eACE) exhibited the highest antioxidant activity. The SH-SY5Y cell line demonstrated significantly higher neuroprotective potential to eACE treatment compared to the U87-MG cell line. Moreover, eACE markedly attenuated intracellular ROS levels and enhanced glutathione peroxidase (GPX) enzymatic activity. In line with these effects, eACE also significantly downregulated the expression of the SNCA gene, suggesting its potential modulatory role in oxidative stress-related neurodegeneration. AC exhibited notable neuroprotective effects in an in vitro model of PD. These effects are likely mediated through the attenuation of oxidative stress and neuroinflammation, inhibition of apoptosis, and preservation of cellular energy metabolism. The results suggest that the extract of AC may provide important insights for developing therapies that modify the progression of PD. Elucidating the underlying mechanisms could inform the creation of innovative treatments designed to slow or alter the trajectory of PD. The present study emphasizes the potential benefits of AC with contemporary scientific investigation to address the unmet needs of patients suffering from PD.

红树Aegiceras corniculatum (AC)是一种被广泛认为具有多种药理特性的红树林植物,近年来因其抗氧化、抗炎和抗菌活性而受到广泛关注。尽管如此,AC的神经保护潜力,特别是在神经退行性疾病如帕金森病(PD)的背景下,仍在很大程度上未被探索。由于人们对植物源性化合物治疗PD的兴趣日益浓厚,研究AC的治疗相关性可以为神经保护的替代策略提供新的见解。本研究旨在评价槐树叶提取物对帕金森病的神经保护作用。进行了初步的植物化学分析,以确定AC提取物(ACE)中存在的生物活性化合物。ACE的抗氧化能力通过三种标准试验进行评估:2,2-二苯基-1-吡啶肼基(DPPH)自由基清除试验、铁还原抗氧化能力(FRAP)试验和过氧化氢(H₂O₂)清除试验。我们在SH-SY5Y人神经母细胞瘤细胞系和U87-MG胶质母细胞瘤细胞系中建立了mptp诱导的PD模型。采用MTT法测定细胞活力,采用2′,7′-二氯荧光素(DCFDA)法测定活性氧(ROS)。采用显色法测定谷胱甘肽过氧化物酶(GPx)活性。Western blot检测SNCA蛋白表达水平。ACE被发现含有多种植物化学物质,包括多酚、类黄酮和萜类化合物。在乙醇、氢乙醇和水提取物中,乙醇ACE (eACE)的抗氧化活性最高。与U87-MG细胞系相比,SH-SY5Y细胞系对eACE治疗表现出更高的神经保护潜力。此外,eACE显著降低细胞内ROS水平,增强谷胱甘肽过氧化物酶(GPX)酶活性。与这些作用一致,eACE也显著下调SNCA基因的表达,提示其在氧化应激相关的神经变性中具有潜在的调节作用。AC在PD体外模型中表现出明显的神经保护作用。这些作用可能是通过氧化应激和神经炎症的衰减、细胞凋亡的抑制和细胞能量代谢的保存介导的。结果表明,AC提取物可能为开发改变PD进展的治疗方法提供重要见解。阐明潜在的机制可以为创造旨在减缓或改变PD轨迹的创新治疗提供信息。本研究强调了AC与当代科学研究的潜在益处,以解决PD患者未满足的需求。
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引用次数: 0
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Applied Biochemistry and Biotechnology
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