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Advantages and Limitations of the Biofilm Study Methods 生物膜研究方法的优势和局限性
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-04-01 DOI: 10.1134/S000368382402011X
D. H. Mir, M. A. Rather
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引用次数: 0
Structural and Functional Impacts of Extended N-Terminal End of the Small Heat Shock Protein Tpv HSP 14.3 小型热休克蛋白 Tpv HSP 14.3 N 端延长的结构和功能影响
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-04-01 DOI: 10.1134/S0003683824020200
S. Zabci, S. Kocabiyik
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引用次数: 0
Biological Activity of Chitosan Nanoparticle Dispersions Produced by Fractional Precipitation 分段沉淀法制备的壳聚糖纳米粒子分散体的生物活性
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-04-01 DOI: 10.1134/s0003683824020145
E. Popova, N. Domnina, I. I. Novikova, N. M. Kovalenko, I. Krasnobaeva, I. M. Zorin
{"title":"Biological Activity of Chitosan Nanoparticle Dispersions Produced by Fractional Precipitation","authors":"E. Popova, N. Domnina, I. I. Novikova, N. M. Kovalenko, I. Krasnobaeva, I. M. Zorin","doi":"10.1134/s0003683824020145","DOIUrl":"https://doi.org/10.1134/s0003683824020145","url":null,"abstract":"","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140782672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Influence of Chitosan on the Ability of LPS to Interact with Cells of the Immune System 壳聚糖对 LPS 与免疫系统细胞相互作用能力的影响
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-04-01 DOI: 10.1134/s0003683824020029
V. Davydova, A. V. Volod’ko, V. I. Gorbach, S. V. Chusovitina, T. F. Solov’eva, I. Yermak
{"title":"The Influence of Chitosan on the Ability of LPS to Interact with Cells of the Immune System","authors":"V. Davydova, A. V. Volod’ko, V. I. Gorbach, S. V. Chusovitina, T. F. Solov’eva, I. Yermak","doi":"10.1134/s0003683824020029","DOIUrl":"https://doi.org/10.1134/s0003683824020029","url":null,"abstract":"","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Biological Efficiencies of Multifunctional Complexes Based on Bacillus subtilis Strains and Chitosan Salicylate in Wheat Cultivation 基于枯草芽孢杆菌菌株和壳聚糖水杨酸盐的多功能复合物在小麦栽培中的生物学效率
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-04-01 DOI: 10.1134/S0003683824020133
I. I. Novikova, L. E. Kolesnikov, E. Popova, B. A. Hassan, N. Priyatkin, D. Y. Radishevskiy, I. Krasnobaeva, L. A. Higerovich, Yu. R. Kolesnikova
{"title":"The Biological Efficiencies of Multifunctional Complexes Based on Bacillus subtilis Strains and Chitosan Salicylate in Wheat Cultivation","authors":"I. I. Novikova, L. E. Kolesnikov, E. Popova, B. A. Hassan, N. Priyatkin, D. Y. Radishevskiy, I. Krasnobaeva, L. A. Higerovich, Yu. R. Kolesnikova","doi":"10.1134/S0003683824020133","DOIUrl":"https://doi.org/10.1134/S0003683824020133","url":null,"abstract":"","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140784221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Bacillus subtilis in Combination with Chitosan Salicylate on Peroxidase and Catalase Activity in B. sorokiniana Infected Wheat 枯草芽孢杆菌与壳聚糖水杨酸盐复配对受 B. sorokiniana 感染小麦过氧化物酶和过氧化氢酶活性的影响
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-04-01 DOI: 10.1134/S0003683824020121
I. I. Novikova, E. Popova, N. M. Kovalenko, I. Krasnobaeva
{"title":"The Effect of Bacillus subtilis in Combination with Chitosan Salicylate on Peroxidase and Catalase Activity in B. sorokiniana Infected Wheat","authors":"I. I. Novikova, E. Popova, N. M. Kovalenko, I. Krasnobaeva","doi":"10.1134/S0003683824020121","DOIUrl":"https://doi.org/10.1134/S0003683824020121","url":null,"abstract":"","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140785677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Disruption of RGD1 Gene Improves Acetic Acid Tolerance in Saccharomyces cerevisiae 干扰 RGD1 基因可提高酿酒酵母对醋酸的耐受性
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-04-01 DOI: 10.1134/S000368382402008X
H. S. Kim
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引用次数: 0
Characterization of Bacterial Isolates from Tailings Pond and Their Resistance to Heavy Metals and Antibiotics 尾矿池细菌分离物的特征及其对重金属和抗生素的耐药性
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-04-01 DOI: 10.1134/S0003683824020157
I. D. Radojević, K. Ćirković, M. Ž. Grujović, K. G. Mladenović, A. M. Ostojić
{"title":"Characterization of Bacterial Isolates from Tailings Pond and Their Resistance to Heavy Metals and Antibiotics","authors":"I. D. Radojević, K. Ćirković, M. Ž. Grujović, K. G. Mladenović, A. M. Ostojić","doi":"10.1134/S0003683824020157","DOIUrl":"https://doi.org/10.1134/S0003683824020157","url":null,"abstract":"","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140760300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolomics Reveals the Impact of Zeolitic Imidazolate Framework-8 on Escherichia coli 代谢组学揭示沸石咪唑酸盐框架-8 对大肠杆菌的影响
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-03-27 DOI: 10.1134/s0003683824700029

Abstract

The increasing use of metal organic frameworks can have many unintended consequences for ecosystems and the environment. However, knowledge of their toxicological behavior and mechanisms of action within the environmental microbial community is extremely limited. In this study, biological and metabolic responses of Escherichia coli to zeolitic imidazolate framework-8 (ZIF-8) were investigated. The results revealed that ZIF-8 could bind to and accumulate on the surface of E. coli cells. Low concentrations of ZIF-8 had little effects on the metabolism of E. coli, while exposure to high-dosage ZIF-8 led to perturbation of cellular functions, including changes of cell membrane permeability, leading to a shortage of energy supply. Moreover, the high-dosage ZIF-8 treatment induced stress-related biological pathways, such as increased metabolism of amino acids, sugars and sugar alcohols. All of these changes resulted in poor growth and low viability of E. coli. Taken together, the results provide detailed picture of the metabolic changes induced by ZIF-8 and are beneficial for assessing the toxicity of ZIF-8 and their further applications.
摘要 越来越多地使用金属有机框架会给生态系统和环境带来许多意想不到的后果。然而，人们对金属有机框架在环境微生物群落中的毒理学行为和作用机制的了解极为有限。本研究调查了大肠杆菌对沸石咪唑啉框架-8（ZIF-8）的生物和代谢反应。结果表明，ZIF-8 可与大肠杆菌细胞结合并在其表面积聚。低浓度的 ZIF-8 对大肠杆菌的新陈代谢影响不大，而接触高剂量的 ZIF-8 则会导致细胞功能紊乱，包括细胞膜通透性发生变化，从而导致能量供应不足。此外，大剂量 ZIF-8 处理诱导了与应激有关的生物通路，如氨基酸、糖和糖醇的代谢增加。所有这些变化都导致大肠杆菌生长不良和活力低下。综上所述，这些结果提供了 ZIF-8 诱导的代谢变化的详细情况，有利于评估 ZIF-8 的毒性及其进一步应用。
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引用次数: 0
Exploring, Cloning, and Characterization of Aryl-alcohol Dehydrogenase for β-Phenylethanol Production in Paocai 探索、克隆和鉴定用于生产β-苯乙醇的芳基乙醇脱氢酶
IF 0.8 4区 生物学 Q3 Immunology and Microbiology
Applied Biochemistry and Microbiology
Pub Date : 2024-03-27 DOI: 10.1134/s0003683823602329
Z. Zhou, Y. Zhao, D. Peng, Z. Wu, W. Zhang

Abstract

β-Phenylethanol is the primary flavor compound in Sichuan industrial paocai. Metatranscriptomics analysis revealed that the gene encoding aryl-alcohol dehydrogenase (AADh1), which is responsible for β-phenylethanol synthesis, had the highest expression level during the late stage of fermentation (180 days). In the present study, we explored the potential AADh1 in Sichuan industrial qingcai paocai by combining metatranscriptomics results and AADh1 analysis in NCBI. AADh1 was successfully cloned and expressed in Escherichia coli BL21, and the enzymatic properties of AADh1 were characterized. AADh1 exhibited the highest activity at pH 7.0 and 50°C, with the highest stability at pH 8.0 and 25°C. Furthermore, the enzyme exhibited low tolerance to metal ions, and only 1 mM Mn2+ could slightly activate the enzyme. Moreover, AADh1 was successfully used to preliminarily enhance the β-phenylethanol of industrial paocai, which could improve the flavor of paocai to a certain extent in practical production.

摘要 β-苯乙醇是四川工业杷菜的主要风味化合物。元转录组学分析表明，负责合成β-苯乙醇的芳基醇脱氢酶（AADh1）基因在发酵后期（180 天）的表达水平最高。在本研究中，我们结合元转录组学结果和 NCBI 中的 AADh1 分析，探讨了四川工业化青菜中潜在的 AADh1。AADh1 被成功克隆并在大肠杆菌 BL21 中表达，AADh1 的酶特性也得到了表征。AADh1 在 pH 值为 7.0 和 50°C 时活性最高，在 pH 值为 8.0 和 25°C 时稳定性最高。此外，该酶对金属离子的耐受性较低，只有 1 mM Mn2+ 才能轻微激活该酶。此外，AADh1 被成功用于初步提高工业杷菜的β-苯乙醇含量，在实际生产中可在一定程度上改善杷菜的风味。
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引用次数: 0
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