Imed Ouni, S. Ammar, Hiba Triki, Imen B. Afia, L. Mansour
Patients with maxillary Kennedy Class I are frequent visitors to the dental office, the missing of posterior teeth makes the control of the movement of removable partial dentures difficult due to the axis of rotation and the different resiliencies between the supporting structures. The use of implants in association to the conventional metal frame denture provides favorable long-term stability and retention, good clinical outcomes in terms of occurrence of complications and maintenance. In this clinical case, a patient with a maxillary Kennedy Class I was rehabilitated using a 3 implants to support metallic removable partial denture. A three dimensional (3D) surgical guide was used for the well-placement of the strategic implants and ball attachments were tightened as connectors between implant and denture. The patient was satisfied after 4-years of follow-up and reported good occlusal stability, esthetic and functional satisfaction.
{"title":"Implant-Supported Removable Partial Denture: An Approach to Rehabilitate Maxillary Kennedy Class I","authors":"Imed Ouni, S. Ammar, Hiba Triki, Imen B. Afia, L. Mansour","doi":"10.17140/doj-7-142","DOIUrl":"https://doi.org/10.17140/doj-7-142","url":null,"abstract":"Patients with maxillary Kennedy Class I are frequent visitors to the dental office, the missing of posterior teeth makes the control of the movement of removable partial dentures difficult due to the axis of rotation and the different resiliencies between the supporting structures. The use of implants in association to the conventional metal frame denture provides favorable long-term stability and retention, good clinical outcomes in terms of occurrence of complications and maintenance. In this clinical case, a patient with a maxillary Kennedy Class I was rehabilitated using a 3 implants to support metallic removable partial denture. A three dimensional (3D) surgical guide was used for the well-placement of the strategic implants and ball attachments were tightened as connectors between implant and denture. The patient was satisfied after 4-years of follow-up and reported good occlusal stability, esthetic and functional satisfaction.","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89445587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Tsubura, Masahide Kanazawa, Shunya Oka, Rika Hirama, T. Tsubura
We describe herein the case of a 72-year-old woman with persistent and symptomatic sore or painful tongue (SPT) treated using PF-MOUTH GELTM (PF-Gel; Daiichi-Sangyo, Osaka, Japan), which includes 30% fucoidan and 0.75% sword bean. PF-MOUTH GELTM was applied to the tongue and kept in place for 3-min. Application was performed twice a day (morning and evening) for 3-months, and resulted in marked improvement of symptomatic sore or painful tongue. Because only one case was reported in this study, clinical trials are required to confirm the efficacy and safety of topical PF-MOUTH GELTM for the treatment of symptomatic sore or painful tongue.
{"title":"Using PF-MOUTH GELTM for Sore or Painful Tongue Improved Symptoms and Stabilized Dryness and Trapping of Food: A Case Report","authors":"S. Tsubura, Masahide Kanazawa, Shunya Oka, Rika Hirama, T. Tsubura","doi":"10.17140/doj-7-143","DOIUrl":"https://doi.org/10.17140/doj-7-143","url":null,"abstract":"We describe herein the case of a 72-year-old woman with persistent and symptomatic sore or painful tongue (SPT) treated using PF-MOUTH GELTM (PF-Gel; Daiichi-Sangyo, Osaka, Japan), which includes 30% fucoidan and 0.75% sword bean. PF-MOUTH GELTM was applied to the tongue and kept in place for 3-min. Application was performed twice a day (morning and evening) for 3-months, and resulted in marked improvement of symptomatic sore or painful tongue. Because only one case was reported in this study, clinical trials are required to confirm the efficacy and safety of topical PF-MOUTH GELTM for the treatment of symptomatic sore or painful tongue.","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90617694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective The main objective of the present study was to do the comparative study of clove oil and clove extract on the oral micro-biota causing dental caries and also to assess the antifungal activity. Materials and Methods The antimicrobial activity of clove oil and clove extract was assessed against Halobacterium sp., Lactobacillus sp., Pseudomonas sp., Micrococcus sp. and Streptococcus mutans (major causative bacteria of dental plaque) by the paper disc diffusion method. For each extract three replicate trials were conducted against each organism. The antifungal activity of clove oil and extract was also assessed against seven fungal species (Aspergillus niger, A. fumigatus, Aspergillus sp., Alternaria sp., Rhizomucor sp., Rhizopus sp. and Penicillium sp.) by agar disc diffusion method. Results Both clove oil and clove extract was found to exhibit broad spectrum of antibacterial activity inhibiting all the ten test bacterial species involved in dental caries. Clove oil produced maximum inhibition zone of diameter (IZD) against themajor causative bacteria of dental plaque as compared to clove extract, thereby, showing that clove oil possesses strong bactericidal activity against oral pathogens. The highest inhibition zone of diameter was observed by clove oil as compared to clove extract against the test fungal species Conclusion The clove oil has the potential to be used as a natural antibacterial agent for oral pathogens.
{"title":"Comparative Study of the Antimicrobial Activity of Clove Oil and Clove Extract on Oral Pathogens","authors":"C. Gupta, D. Prakash","doi":"10.17140/doj-7-144","DOIUrl":"https://doi.org/10.17140/doj-7-144","url":null,"abstract":"Objective The main objective of the present study was to do the comparative study of clove oil and clove extract on the oral micro-biota causing dental caries and also to assess the antifungal activity. Materials and Methods The antimicrobial activity of clove oil and clove extract was assessed against Halobacterium sp., Lactobacillus sp., Pseudomonas sp., Micrococcus sp. and Streptococcus mutans (major causative bacteria of dental plaque) by the paper disc diffusion method. For each extract three replicate trials were conducted against each organism. The antifungal activity of clove oil and extract was also assessed against seven fungal species (Aspergillus niger, A. fumigatus, Aspergillus sp., Alternaria sp., Rhizomucor sp., Rhizopus sp. and Penicillium sp.) by agar disc diffusion method. Results Both clove oil and clove extract was found to exhibit broad spectrum of antibacterial activity inhibiting all the ten test bacterial species involved in dental caries. Clove oil produced maximum inhibition zone of diameter (IZD) against themajor causative bacteria of dental plaque as compared to clove extract, thereby, showing that clove oil possesses strong bactericidal activity against oral pathogens. The highest inhibition zone of diameter was observed by clove oil as compared to clove extract against the test fungal species Conclusion The clove oil has the potential to be used as a natural antibacterial agent for oral pathogens.","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73218159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Placing an implant to replace missing teeth can be challenging because of lack of vertical and/or horizontal bone ridge, maxillary sinus pneumatization and inferior alveolar nerve position. Additional surgical procedures may be necessary, with varying predictability, where vertical augmentation being the least predictable. An alternative option is to place short implants and exclude the additional surgical grafting procedures. By reviewing studies, this paper explores the predictability of the short implants for use in the methods. Bicon SHORT® implants are available in 5 and 6 mm lengths and seem to be able to overcome such limits but more long-term studies are still needed to determine long-term prognosis and success of short implants in terms of them being comparable or equal to longer or standard length implants.
{"title":"A Clinical Paradigm and Pertinent Literature Review for Placing Short Implants","authors":"S. Yao, ames B. Fine","doi":"10.17140/doj-7-145","DOIUrl":"https://doi.org/10.17140/doj-7-145","url":null,"abstract":"Placing an implant to replace missing teeth can be challenging because of lack of vertical and/or horizontal bone ridge, maxillary sinus pneumatization and inferior alveolar nerve position. Additional surgical procedures may be necessary, with varying predictability, where vertical augmentation being the least predictable. An alternative option is to place short implants and exclude the additional surgical grafting procedures. By reviewing studies, this paper explores the predictability of the short implants for use in the methods. Bicon SHORT® implants are available in 5 and 6 mm lengths and seem to be able to overcome such limits but more long-term studies are still needed to determine long-term prognosis and success of short implants in terms of them being comparable or equal to longer or standard length implants.","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73667118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peer Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Dentistry Journal maintains its standards for the high quality of its published papers [...].
{"title":"Acknowledgment to Reviewers of Dentistry Journal in 2020","authors":"Dentistry Journal Editorial Office","doi":"10.3390/dj9020015","DOIUrl":"https://doi.org/10.3390/dj9020015","url":null,"abstract":"Peer Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Dentistry Journal maintains its standards for the high quality of its published papers [...].","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88578504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The editorial team greatly appreciates the reviewers who have dedicated their[...].
编辑团队非常感谢为[…]做出贡献的审稿人。
{"title":"Acknowledgement to Reviewers of Dentistry Journal in 2019","authors":"Dentistry Journal Editorial Office","doi":"10.3390/dj8010013","DOIUrl":"https://doi.org/10.3390/dj8010013","url":null,"abstract":"The editorial team greatly appreciates the reviewers who have dedicated their[...].","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2020-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77023280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Periodontal destruction observed in restored teeth is strongly caused by marginal discrepancy of the restoration, which is closely influenced by the tooth preparation, the technique of retraction and impression. Materials and Methods A hundred practitioners were selected to constitute the study’s sample. A self-administered questionnaire survey was carried out. The questionnaire was filled in the practitioner's office, completed in the absence of the investigator. However, it has been completed for some clarifications by an interview. A digital form made through the Google Forms application provided free by the Google search engine. The questionnaire included two sections: The identification of the practitioner: this is general information about the practitioner. The conduct of prosthetic treatment. The causes of marginal discrepancy: This section concerns the major factors responsible for a good marginal fit. The collected data was introduced and processed by the microcomputer using the statistical software XLSTAT 2015 for Windows. A simple statistical analysis made it possible to calculate the percentage of the different variables, from their frequency. Results There is a significant correlation between marginal discrepancy and the respect of the finish line geometry (p=0.001). The technique of retraction which provided the best marginal fit was the use of expasyl paste (p=0.18). There was a significant association between marginal discrepancy and the material of impression, marginal adaptation is found in 76% for impressions made by alginate, 45% for impressions made by silicone, and 100% for impressions made by polysulphides (p=0.01). Conclusion Within limitations of this study, it can be concluded that the respect of guidelines of preparation especially the finish line and the good choice of the retraction technique and the material of impression are the major keys to have a good marginal fit.
目的观察修复牙的牙周破坏主要是由修复体的边缘差异引起的，而边缘差异与牙体的预备、内缩和印模技术密切相关。材料与方法选取100名从业人员作为研究样本。进行了一项自我管理的问卷调查。问卷是在医生的办公室填写的，在调查员不在场的情况下完成。然而，它已经完成了一些澄清采访。通过谷歌搜索引擎免费提供的谷歌表单应用程序制作的数字表单。问卷包括两个部分:从业者的识别:这是关于从业者的一般信息。义肢治疗的进行边际差异的原因:这一节涉及到造成良好边际拟合的主要因素。利用XLSTAT 2015 for Windows统计软件对采集的数据进行了介绍和处理。一个简单的统计分析可以计算出不同变量的百分比，从他们的频率。结果边际差异与终点线几何形状之间存在显著相关(p=0.001)。使用expasyl膏体是提供最佳边缘拟合的后缩技术(p=0.18)。边际差异与印模材料之间存在显著关联，海藻酸盐印模的边际适应性为76%，硅胶印模为45%，聚硫化物印模为100% (p=0.01)。结论在本研究的局限性下，对预备指导原则特别是终点线的尊重、对牵入技术和印模材料的选择是获得良好边缘配合的关键。
{"title":"The Causes of Marginal Discrepancy of Fixed Dental Prostheses: A Cross-Sectional Study","authors":"A. Ismail, Y. Gassara, D. Hadyaoui, M. Chérif","doi":"10.17140/doj-5-141","DOIUrl":"https://doi.org/10.17140/doj-5-141","url":null,"abstract":"Objective Periodontal destruction observed in restored teeth is strongly caused by marginal discrepancy of the restoration, which is closely influenced by the tooth preparation, the technique of retraction and impression. Materials and Methods A hundred practitioners were selected to constitute the study’s sample. A self-administered questionnaire survey was carried out. The questionnaire was filled in the practitioner's office, completed in the absence of the investigator. However, it has been completed for some clarifications by an interview. A digital form made through the Google Forms application provided free by the Google search engine. The questionnaire included two sections: The identification of the practitioner: this is general information about the practitioner. The conduct of prosthetic treatment. The causes of marginal discrepancy: This section concerns the major factors responsible for a good marginal fit. The collected data was introduced and processed by the microcomputer using the statistical software XLSTAT 2015 for Windows. A simple statistical analysis made it possible to calculate the percentage of the different variables, from their frequency. Results There is a significant correlation between marginal discrepancy and the respect of the finish line geometry (p=0.001). The technique of retraction which provided the best marginal fit was the use of expasyl paste (p=0.18). There was a significant association between marginal discrepancy and the material of impression, marginal adaptation is found in 76% for impressions made by alginate, 45% for impressions made by silicone, and 100% for impressions made by polysulphides (p=0.01). Conclusion Within limitations of this study, it can be concluded that the respect of guidelines of preparation especially the finish line and the good choice of the retraction technique and the material of impression are the major keys to have a good marginal fit.","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2019-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79799699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As the population ages, both Alzheimer’s disease and periodontal disease may increase in incidence. Both do not have a cure and can affect quality of life. Research is being conducted to determine causes, treatments and potential relationship to each other. Proposed mechanisms to how they are related is via inflammation and/or bacteria. Several studies have addressed if dental treatment could improve cognitive function. However, further research is needed to further elucidate the relationship between both diseases. Common therapeutic approaches may help to manage both conditions, determine susceptibility and possibly prevention.
{"title":"Dementia and Oral Health: Is There A Connection?","authors":"S. Yao, J. Fine","doi":"10.17140/doj-5-140","DOIUrl":"https://doi.org/10.17140/doj-5-140","url":null,"abstract":"As the population ages, both Alzheimer’s disease and periodontal disease may increase in incidence. Both do not have a cure and can affect quality of life. Research is being conducted to determine causes, treatments and potential relationship to each other. Proposed mechanisms to how they are related is via inflammation and/or bacteria. Several studies have addressed if dental treatment could improve cognitive function. However, further research is needed to further elucidate the relationship between both diseases. Common therapeutic approaches may help to manage both conditions, determine susceptibility and possibly prevention.","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2019-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84113435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Binaljadm, R. Moorehead, Thafar Almela, Kirsty L. Franklin, L. Tayebi, K. Moharamzadeh
Restoring subgingival class-V cavities successfully, demand special biological properties from a restorative material. This study aimed to assess the effects of incorporating bioactive materials to glass ionomer cement (GIC) on its mechanical and biological properties. Hydroxyapatite, chitosan, chondroitin sulphate, bioglass, gelatine and processed bovine dentin were incorporated into a GIC restorative material. Compressive strength, biaxial flexural strength (BFS), hardness, setting and working time measurements were investigated. Biocompatibility of the new materials was assessed using both monolayer cell cultures of normal oral fibroblasts (NOF) and TR146 keratinocytes, and a 3D-tissue engineered human oral mucosa model (3D-OMM) using presto-blue tissue viability assay and histological examination. Significant reduction in the compressive strength and BFS of gelatine-modified discs was observed, while chondroitin sulphate-modified discs had reduced BFS only (p value > 0.05). For hardness, working and setting times, only bioglass caused significant increase in the working time. NOF viability was significantly increased when exposed to GIC-modified with bovine dentine, bioglass and chitosan. Histological examination showed curling and growth of the epithelial layer toward the disc space, except for the GIC modified with gelatine. This study has highlighted the potential for clinical application of the modified GICs with hydroxyapatite, chitosan, bioglass and bovine dentine in subgingival class-V restorations.
{"title":"Biomodification of a Class-V Restorative Material by Incorporation of Bioactive Agents","authors":"T. Binaljadm, R. Moorehead, Thafar Almela, Kirsty L. Franklin, L. Tayebi, K. Moharamzadeh","doi":"10.3390/dj7040110","DOIUrl":"https://doi.org/10.3390/dj7040110","url":null,"abstract":"Restoring subgingival class-V cavities successfully, demand special biological properties from a restorative material. This study aimed to assess the effects of incorporating bioactive materials to glass ionomer cement (GIC) on its mechanical and biological properties. Hydroxyapatite, chitosan, chondroitin sulphate, bioglass, gelatine and processed bovine dentin were incorporated into a GIC restorative material. Compressive strength, biaxial flexural strength (BFS), hardness, setting and working time measurements were investigated. Biocompatibility of the new materials was assessed using both monolayer cell cultures of normal oral fibroblasts (NOF) and TR146 keratinocytes, and a 3D-tissue engineered human oral mucosa model (3D-OMM) using presto-blue tissue viability assay and histological examination. Significant reduction in the compressive strength and BFS of gelatine-modified discs was observed, while chondroitin sulphate-modified discs had reduced BFS only (p value > 0.05). For hardness, working and setting times, only bioglass caused significant increase in the working time. NOF viability was significantly increased when exposed to GIC-modified with bovine dentine, bioglass and chitosan. Histological examination showed curling and growth of the epithelial layer toward the disc space, except for the GIC modified with gelatine. This study has highlighted the potential for clinical application of the modified GICs with hydroxyapatite, chitosan, bioglass and bovine dentine in subgingival class-V restorations.","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2019-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83633881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Tsujino, Akira Takahashi, Taisuke Watanabe, K. Isobe, Y. Kitamura, K. Okuda, K. Nakata, T. Kawase
Recent progress in the industrial development of dental implants has improved their surface bio-affinity, while clinical implantologists attempt to improve it through coating with various compounds, including platelet-rich plasma (PRP) in clinical settings. However, it is poorly understood how PRP acts on titanium surfaces. To validate this surface modification method and demonstrate how platelet-derived soluble biomolecules released from the activated adherent platelets act on plain, commercially pure-titanium (cp-Ti) plates, we evaluated the distribution of biomolecules by immunofluorescence. PPARγ, PDGF-B, and TGFβ1 were similarly released at immunofluorescence levels from activated adherent platelets, retained in the surrounding extra-platelet spaces for a while, and did not immediately diffuse away to distant spaces. Exogenously added CaCl2 augmented release and retention of those biomolecules along with activation and aggregation. Taken together with our previous data regarding platelet adhesion, these findings suggest that especially when treated with CaCl2, platelets immediately adhere on cp-Ti plates to release their stored biomolecules in the absence of plasma proteins and that these biomolecules do not diffuse away, but stay longer in extra-platelet spaces around the platelets by newly formed, immature fibrin fiber fragments. Consequently, these retained biomolecules are anticipated to cooperatively stabilize implants by stimulating alveolar bone regeneration and integration.
{"title":"Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets","authors":"T. Tsujino, Akira Takahashi, Taisuke Watanabe, K. Isobe, Y. Kitamura, K. Okuda, K. Nakata, T. Kawase","doi":"10.3390/dj7040109","DOIUrl":"https://doi.org/10.3390/dj7040109","url":null,"abstract":"Recent progress in the industrial development of dental implants has improved their surface bio-affinity, while clinical implantologists attempt to improve it through coating with various compounds, including platelet-rich plasma (PRP) in clinical settings. However, it is poorly understood how PRP acts on titanium surfaces. To validate this surface modification method and demonstrate how platelet-derived soluble biomolecules released from the activated adherent platelets act on plain, commercially pure-titanium (cp-Ti) plates, we evaluated the distribution of biomolecules by immunofluorescence. PPARγ, PDGF-B, and TGFβ1 were similarly released at immunofluorescence levels from activated adherent platelets, retained in the surrounding extra-platelet spaces for a while, and did not immediately diffuse away to distant spaces. Exogenously added CaCl2 augmented release and retention of those biomolecules along with activation and aggregation. Taken together with our previous data regarding platelet adhesion, these findings suggest that especially when treated with CaCl2, platelets immediately adhere on cp-Ti plates to release their stored biomolecules in the absence of plasma proteins and that these biomolecules do not diffuse away, but stay longer in extra-platelet spaces around the platelets by newly formed, immature fibrin fiber fragments. Consequently, these retained biomolecules are anticipated to cooperatively stabilize implants by stimulating alveolar bone regeneration and integration.","PeriodicalId":47284,"journal":{"name":"Open Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2019-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80286015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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