Biology-Basel最新文献

Drought stress constitutes a major challenge to wheat production. Melatonin plays a vital role in plants' resistance to drought stress. Nevertheless, the influence of melatonin seed coating on the drought resistance ability of wheat remains unclear. Hence, in this study, wheat (Yunmai 112) was chosen as the experimental material. The research results indicated that 100 µM exogenous melatonin treatment enhanced the germination rate of wheat seeds by 11% compared to the CK group. Melatonin seed coating (100 or 200 µM) significantly inhibited the accumulation of ROS in wheat seedlings under drought stress conditions and facilitated the growth of wheat seedlings. Then, 100 µM melatonin seed coating elevated the activity of antioxidant enzymes (CAT, Cu/Zn-SOD, POD, and T-GSH) in wheat seedlings and strengthened the resistance of wheat to drought stress. In contrast to the control, 100 or 200 µM melatonin seed coating significantly raised the contents of soluble protein and chlorophyll in wheat seedlings. Further studies demonstrated that 100 µM melatonin seed coating promoted the increase in the thousand-grain weight and yield of wheat under drought stress. Taking together, melatonin seed coating is an effective approach for enhancing the stress resistance and the yield of wheat under drought stress.

Neural oscillations observed during semantic processing embody the function of brain language processing. Precise parameterization of the differences in these oscillations across various semantics from a time-frequency perspective is pivotal for elucidating the mechanisms of brain language processing. The superlet transform and cluster depth test were used to compute the time-frequency representation of oscillatory difference (ODTFR) between neural activities recorded by optically pumped magnetometer-based magnetoencephalography (OPM-MEG) during processing congruent and incongruent Chinese semantics. Subsequently, ODTFR was parameterized based on the definition of local events. Finally, this study calculated the specific time-frequency values at which oscillation differences occurred in multiple auditory-language-processing regions. It was found that these oscillatory differences appeared in most regions and were mainly concentrated in the beta band. The average peak frequency of these oscillatory differences was 15.7 Hz, and the average peak time was 457 ms. These findings offer a fresh perspective on the neural mechanisms underlying the processing of distinct Chinese semantics and provide references and insights for analyzing language-related brain activities recorded by OPM-MEG.

In 2001, two enzyme-encoding genes were recognized in the fruit fly Drosophila melanogaster. The genetic material, labeled Dicer-1 and Dicer-2, encodes ribonuclease-type enzymes with slightly diverse target substrates. The human orthologue is DICER1. It is a gene, which has been positioned on chromosome 14q32.13. It contains 27 exons, which are linking the two enzyme domains. DICER1 is found in all organ systems. It has been proved that it is paramount in human development. The protein determined by DICER1 is a ribonuclease (RNase). This RNase belongs to the RNase III superfamily, formally known as 'endoribonuclease'. It has been determined that the function of RNase III proteins is set to identify and degrade double-stranded molecules of RNA. DICER1 is a vital "housekeeping" gene. The multi-domain enzyme is key for small RNA processing. This enzyme functions in numerous pathways, including RNA interference paths, DNA damage renovation, and response to viruses. At the protein level, DICER is also involved in several human diseases, of which the pleuro-pulmonary blastoma is probably the most egregious entity. Numerous studies have determined the full range of DICER1 functions and the corresponding relationship to tumorigenic and non-neoplastic diseases. In fact, genetic mutations (somatic and germline) have been detected in DICER1 and are genetically associated with at least two clinical syndromes: DICER1 syndrome and GLOW syndrome. The ubiquity of this enzyme in the human body makes it an exquisite target for nanotechnology-supported therapies and repurposing drug approaches.

Advancing our understanding of pancreatic toxicity and metabolic disorders caused by environmental exposures requires innovative approaches. The pancreas, a vital organ for glucose regulation, is increasingly recognized as a target of harm from environmental chemicals and dietary factors. Traditional toxicological methods, while foundational, often fail to address the mechanistic complexities of pancreatic dysfunction, particularly under real-world conditions involving multiple exposures. New Approach Methodologies (NAMs)-including high-throughput screening (HTS), OMICS technologies, computational modeling, and advanced in vitro systems-offer transformative tools to tackle these challenges. NAMs enable the identification of mechanistic pathways, improve testing efficiency, and reduce reliance on animal testing. This commentary explores the integration of NAMs into pancreatic toxicity screening, addresses critical gaps in evaluating the cumulative risks of chemical and dietary exposures, and proposes solutions for integrating the pancreas into toxicity screening through NAMs. By highlighting recent advancements and emphasizing their adoption in environmental toxicity assessment frameworks, this work demonstrates the potential of NAMs to revolutionize environmental health research, inspire interdisciplinary collaboration, and protect public health.

The Aloe barbadensis industry plays an important role in the economic development of Yuanjiang county of Yuxi city in Yunnan province, China. In order to reduce the harm of diseases and ensure the quality of products, the occurrence of A. barbadensis was investigated. The pathogenic fungi of wild and cultivated species of A. barbadensis were isolated by a tissue separation method, and DNA sequencing was carried out by using the sequence analysis of the ribosomal rDNA-ITS region, and the pathogenic fungi were classified and identified by finally combining morphological observations. The results showed that the main fungal diseases were root rot and leaf rot disease caused by Fusarium oxysporum, leaf spot disease caused by Curvularia lunata, anthracnose caused by Colletotrichum boninense, and brown spot disease caused by Alternaria alternata on A. barbadensis.

Marine hydrothermal ecosystems represent extreme environments connected to submarine volcanic areas characterized by vents, having high temperatures and particular chemical compositions. The hydrothermal marine system of Panarea, located in one of the seven small islands belonging to the Aeolian Archipelago (southern Tyrrhenian Sea), is characterized by a range of vents exhibiting diverse physical and chemical conditions. We aimed to analyze the microbial community of a peculiar hot spring belonging to the Panarea hydrothermal field, known as "Black Point" (BP), in two separate sampling expeditions (May and August). Our results demonstrated that the chemical-physical variations within this hydrothermal vent, such as temperature fluctuations, mineral content, and hydrothermal fluid dynamics, play a role in shaping the structure and diversity of microbial communities. The differences between the two sampling expeditions suggest that seasonal changes, i.e., in temperature, pH, and redox potential (Eh), could drive microbial community shifts over time.

Oxidative stress, a critical factor affecting the health and productivity of aquatic organisms, arises from the imbalance between reactive oxygen species (ROS) production and antioxidant defenses. In aquaculture, natural antioxidants have gained increasing attention as sustainable feed additives to mitigate oxidative damage, enhance immune responses, and improve overall growth performance. This review provides a comprehensive synthesis of the antioxidative mechanisms of key natural antioxidants, including carotenoids, polysaccharides, vitamins, polyphenols, and flavonoids. By neutralizing ROS and modulating cellular signaling pathways such as Nrf2/ARE, these compounds offer significant protective effects against oxidative damage in aquatic species. The manuscript consolidates recent advancements in antioxidant research, highlighting their practical applications in feed formulation and their role in promoting sustainability in aquaculture. This review aims to provide an integrative framework for understanding natural antioxidants' potential, guiding future research and practical implementation in aquaculture systems.

This study seeks to assess the impact of varying concentrations of lysophospholipids on the antioxidant capacity, digestive performance, and intestinal microbiota of L. vannamei. A total of 840 shrimp, with an average initial weight of 2.22 ± 0.11 g, were randomly distributed across 28 aquaculture tanks (30 shrimp per tank), organized into 7 distinct treatment groups, each comprising 4 replicates. The control group (DL2) was administered a basal diet formula supplemented with 2% soy lecithin, whereas the experimental groups received lysophospholipids at varying concentrations of 0% (RL0), 0.1% (RL0.1), 0.5% (RL0.5), 1% (RL1), 1.5% (RL1.5), and 2% (RL2). The results revealed that the total antioxidant capacity (T-AOC) level in the RL0.1 group was significantly elevated compared to the DL2 and RL2 groups (p < 0.05). Furthermore, glutathione peroxidase (GSH-Px) and catalase (CAT) activities were markedly higher in the RL1 and RL1.5 groups relative to other groups (p < 0.05). Superoxide dismutase (SOD) activity exhibited a significant increase across all lysophospholipid-supplemented groups when compared to the DL2 group (p < 0.05). Malondialdehyde (MDA) levels were notably elevated in the RL0.5, RL1.5, and RL2 groups relative to the other groups (p < 0.05). Regarding antioxidant-related genes in the hemocytes, the relative expression levels of Nrf1, Nrf2, GPx, SOD, CAT, and Hippo were significantly higher in the RL0.5 and RL1 groups compared to the DL2 group (p < 0.05). Additionally, the relative expression levels of GPx and SOD were significantly elevated in the RL2 group compared to the DL2 group (p < 0.05). In the intestinal tract, the activities of trypsin and α-amylase (AMS) were significantly elevated in the RL0.1, RL0.5, and RL1 groups compared to the DL2 group (p < 0.05). Additionally, the RL0.1 group demonstrated significantly higher lipase (LPS) activity than the other groups supplemented with lysophospholipids (p < 0.05). Furthermore, the relative expression levels of the trypsin and α-amylase genes were significantly increased in the RL1 and RL1.5 groups in comparison to the DL2 group (p < 0.05). Sequencing analysis of the intestinal microbiota indicated that the incorporation of lysophospholipids resulted in an enhancement of the composition and structure of the intestinal microbiota. The functional abundance of the intestinal microbiome was primarily enriched in metabolic pathways. Overall, the incorporation of lysophospholipids into the diet exerted a beneficial effect on the antioxidant capacity, digestive performance, and intestinal microbiota of L. vannamei, especially with the supplementation of 0.1% lysophospholipids.

This study investigated the surface microbiome of the honeybee (Apis mellifera), focusing on the diversity and functional roles of its associated microbial communities. While the significance of the microbiome to insect health and behavior is increasingly recognized, research on invertebrate surface microbiota lags behind that of vertebrates. A combined metagenomic and cultivation-based approach was employed to characterize the bacterial communities inhabiting the honeybee exoskeleton. Our findings reveal a complex and diverse microbiota exhibiting significant spatial and environmental heterogeneity. The identification of antimicrobial compound producers, validated through both culture and metagenomic analyses, including potentially novel Actinobacteria species, underscores the potential impact of these microbial communities on honeybee health, behavior, and hive dynamics. This research contributes to a more profound ecological understanding of the honeybee microbiome, particularly in its winter configuration.

Interactions between plants and their endophytes alter their metabolic functions and ability to cope with abiotic stresses. In this study, high-throughput sequencing was used to analyze the species diversity and functions of endophytes in Cyperus esculentus var. sativus (CES) tubers under different heavy metal stress conditions. The results indicated that the number of observed endophytic species in the tubers increased under heavy metal stress (p < 0.05), leading to changes in species diversity and composition. The response of tuber endophytes to different metal concentrations varied, with certain endophytic bacteria and fungi, such as Pseudomonas, Novosphingobium, and Fusarium, showing increased abundance and becoming the dominant species in the tubers. Additionally, new endophytic genera, Actinophytocola and Monosporascus, emerged at specific metal concentrations (p < 0.05). Fatty acid salvage was enriched in the endophytes of CES, which may play an important role in assisting CES in responding to multiple heavy metal stresses. These findings showed that CES tuber endophytes undergo adaptive changes to support the ability of plants to cope with heavy metal stress.