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Exploring the performance of amaranth grain starch and protein microcapsules as β-carotene carrier systems for food applications 苋粒淀粉和蛋白质微胶囊作为β-胡萝卜素食品载体体系的性能研究
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.foostr.2022.100287
Laylla Marques Coelho , Idalina Gonçalves , Paula Ferreira , Ana C. Pinheiro , António A. Vicente , Joana T. Martins

Underexploited sources of bio-based wall materials for bioactive compounds (such as β-carotene) encapsulation have gained increasing interest within the scientific community. In this study, the potential of amaranth (Amaranthus cruentus) grain starch and protein rich fractions as microcapsules’ wall materials to carrier β-carotene was evaluated. Microcapsules were produced by spray-drying and their morphological and physicochemical characterisation was carried out. The microcapsules presented a spherical shape (particle size distribution: 0.3–30 µm) and encapsulation efficiencies ranging from 64 % to 69 %. Results showed that protein-based microcapsules had better β-carotene storage stability as compared to starch-based microcapsules (at 8 and 25 °C). β-carotene release kinetics at 37 °C and pH 7.4 could be mainly described by structural-relaxation phenomenon using the linear superposition model. Moreover, encapsulated β-carotene exhibited higher bioaccessibility than its free form after simulated in vitro digestion tests. Microcapsules did not affect cell viability at 0.0625 mg L−1 of β-carotene. Thus, amaranth grain biopolymers-based microcapsules were successfully developed as promising β-carotene delivery systems to be added to food products and consequently, to improve their functionality.

未开发的生物活性化合物(如β-胡萝卜素)包封的生物基壁材料来源在科学界引起了越来越大的兴趣。本研究评价了苋菜(Amaranthus cruentus)籽粒淀粉和蛋白质丰富组分作为微胶囊壁材载体β-胡萝卜素的潜力。采用喷雾干燥法制备微胶囊,并对微胶囊进行了形态和理化表征。微胶囊呈球形(粒径分布为0.3 ~ 30µm),包封效率为64% ~ 69%。结果表明,与淀粉基微胶囊相比,蛋白质基微胶囊在8°C和25°C下具有更好的β-胡萝卜素储存稳定性。在37℃和pH 7.4条件下,β-胡萝卜素的释放动力学可以用线性叠加模型描述为结构松弛现象。体外模拟消化实验表明,包封的β-胡萝卜素比游离的β-胡萝卜素具有更高的生物可及性。微胶囊在0.0625 mg L−1 β-胡萝卜素浓度下不影响细胞活力。因此,基于苋菜颗粒生物聚合物的微胶囊被成功开发为有前途的β-胡萝卜素递送系统,可以添加到食品中,从而提高其功能。
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引用次数: 8
Effects of thermal-induced konjac glucomannan-protein interaction on structural and rheological properties of wheat dough 热诱导魔芋葡甘露蛋白相互作用对小麦面团结构和流变特性的影响
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.foostr.2022.100288
Tingting Cui , Xiaodan Zhou , Wenjie Sui , Rui Liu , Tao Wu , Shuai Wang , Yan Jin , Min Zhang

This work is dealt with how structural changes induced by konjac glucomannan (KGM) interacting with wheat protein contribute to dough properties upon the heating stage. It comparatively investigated the effects of KGM on gluten thermal-polymerization process from molecular weight, free sulfhydryl content, secondary structure and thermal stability, and then analyzed KGM-induced changes of structural and rheological properties of wheat dough with heating temperature increased. Results showed that KGM increased the average molecular weight, the β-sheet/β-turn ratio and denaturation temperature of gluten protein while decreased the content of free sulfhydryl groups. It was likely that KGM promoted the formation and stability of gluten networks upon heating by interfering sulfydryl interchange reactions and thermal denaturation. Dough structure was thus strengthened to a compact network and presented the increased elasticity and the raised resilience, springiness, cohesiveness and chewiness of textural properties by heating treatment.

本文研究了魔芋葡甘露聚糖(KGM)与小麦蛋白相互作用所引起的结构变化对加热阶段面团性质的影响。从分子量、游离巯基含量、二级结构和热稳定性等方面比较研究了KGM对面筋热聚合过程的影响,分析了KGM对小麦面团结构和流变学性能随加热温度升高的影响。结果表明:KGM提高了面筋蛋白的平均分子量、β-片/β-转比和变性温度,降低了游离巯基含量;KGM可能通过干扰巯基交换反应和热变性来促进面筋网络的形成和稳定性。热处理后的面团结构被强化为致密的网状结构,呈现出弹性增加、回弹性、弹性、黏结性和咀嚼性等质地性能提高的特点。
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引用次数: 4
Stability, rheological properties and microstructure of Pickering emulsions stabilized by different concentration of glidian/sodium caseinate nanoparticles using konjac glucomannan as structural regulator 以魔芋葡甘露聚糖为结构调节剂的格利啶酮/酪蛋白酸钠纳米颗粒稳定皮克林乳的稳定性、流变性能和微观结构
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.foostr.2022.100285
Wei Xu , Shuqing Zheng , Wenjie Xi , Haomin Sun , Yuli Ning , Yin Jia , Denglin Luo , Yingying Li , Bakht Ramin Shah

In this paper, the effect of konjac glucomannan (KGM) on the physical stability, rheological properties, and microstructure of Pickering emulsions stabilized by gliadin/sodium caseinate nanoparticles (Gli/CAS NPs) with different concentration was investigated. When the Gli/CAS NPs concentration was constant, the increase of KGM content significantly improved the storage stability of the emulsion. For emulsion samples with 20% oil content, with the increase of KGM concentration from 0.1% to 0.6%, the D32 value decreased from 7.57 ± 0.24 µm to 4.47 ± 0.10 µm. The CLSM and cryo-SEM observations showed that the particles adsorbed on the oil-water interface and formed a network structure. The structure was conducive to the stability of the emulsion. Irregular bulges on the surface of emulsion with higher KGM content could be observed. When the Gli/CAS NPs concentration was 4 wt%, the viscoelasticity of emulsion was improved and the G′ and G" value of the emulsion increases with the increase of KGM content. The study showed that KGM has the potential to regulate the stability and rheological properties of emulsions, which might provide an interesting perspective for various industrial applications of functional emulsions.

本文研究了魔芋葡甘露聚糖(KGM)对不同浓度麦胶蛋白/酪蛋白酸钠纳米颗粒(Gli/CAS NPs)稳定的皮克林乳的物理稳定性、流变性能和微观结构的影响。当Gli/CAS NPs浓度一定时,KGM含量的增加显著提高了乳状液的贮存稳定性。对于含油量为20%的乳化液样品,随着KGM浓度从0.1%增加到0.6%,D32值从7.57±0.24µm下降到4.47±0.10µm。CLSM和低温扫描电镜观察表明,颗粒吸附在油水界面上,形成网状结构。该结构有利于乳液的稳定性。KGM含量较高的乳化液表面出现不规则凸起。当Gli/CAS NPs浓度为4 wt%时,乳液的粘弹性得到改善,且随着KGM含量的增加,乳液的G′和G”值增大。研究表明,KGM具有调节乳剂稳定性和流变性能的潜力,这可能为功能乳剂的各种工业应用提供一个有趣的前景。
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引用次数: 11
Use of discrimination analysis to identify differences during cooking of novel pasta formulations 利用判别分析识别新型意大利面配方烹饪过程中的差异
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.foostr.2022.100291
F Boukid , P Littardi , E Carini , A Diantom , E Curti , Y Vodovotz , E Vittadini

Given the great innovation in pasta formulations, elucidating factors that will impact pasta behaviour during cooking is essential when alternative ingredients are incorporated. Whole wheat (W), vegetable (V) and gluten free (GF) pastas (from raw to overcooked) were analysed using a multiscale approach and compared with a standard (STD) formulation. Macroscopic (moisture content and hardness), mesoscopic (viscoelastic properties and degree of gelatinization) and molecular (1H NMR relaxometry) properties were evaluated and coupled with discrimination analysis (by means of principal components analysis and partial least square). Results from 2-ways ANOVA indicated that the cooking time (CT) was the main factor influencing the studied properties overlapping the effect of pasta formulation (PF). The application of partial least square analysis was effective in indicating viscoelastic properties and several molecular mobility indicators as typifying features able to describe pasta behaviour during cooking and discriminating GF from their gluten-containing counterparts.

考虑到意大利面配方的巨大创新,在加入替代成分时,阐明影响意大利面在烹饪过程中的行为的因素是必不可少的。采用多尺度方法对全麦(W)、蔬菜(V)和无麸质(GF)意大利面(从生的到煮熟的)进行了分析,并与标准(STD)配方进行了比较。对其宏观(含水率和硬度)、介观(粘弹性和糊化程度)和分子(1H NMR弛豫)性能进行了评价,并结合判别分析(通过主成分分析和偏最小二乘法)。双因素方差分析结果表明,蒸煮时间(CT)是影响面食配方(PF)性能的主要因素。偏最小二乘分析的应用可以有效地表明粘弹性特性和一些分子迁移率指标作为描述面食在烹饪过程中的行为的典型特征,并将GF与麸质区分开来。
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引用次数: 0
Editorial: Special issue on multiscale simulations and experimental characterization of structured foods 社论:关于结构化食品的多尺度模拟和实验表征的特刊
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.foostr.2022.100283
Ruud van der Sman, John van Duynhoven
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引用次数: 1
Utilization of emulsion technology to create plant-based adipose tissue analogs: Soy-based high internal phase emulsions 利用乳剂技术制备植物性脂肪组织类似物:大豆高内相乳剂
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.foostr.2022.100290
Xiaoyan Hu, Hualu Zhou, David Julian McClements

High-quality plant-based foods like meat and fish analogs should have physicochemical attributes, such as their look, feel, and taste, that mimic those of the animal-based products they replace. This study focused on the development of plant-based adipose tissue using advanced emulsion technologies. Oil-in-water high internal phase emulsions (HIPEs) assembled from soybean oil (60–85%) and soybean protein (2 wt%) were used to simulate the optical and rheological properties of beef adipose tissue. The microstructure and appearance of HIPEs containing 75 or 80% oil were like those of beef adipose tissue. However, the diameter of the adipocytes was around 100 µm in the adipose tissue, whereas the diameter of the fat droplets in the HIPEs was only around 10 µm. The shear rheology of the HIPEs and adipose tissue were similar at high temperatures (> 60 °C) but beef adipose was much harder at lower temperatures, which was attributed to fat crystallization. The hardness of the HIPEs increased with increasing fat content from 60% to 80% but then decreased when it was further raised to 85% because the emulsion broke down. The plant-based adipose tissue developed here may be useful for creating certain kinds of meat or fish analogs.

优质的植物性食品,如肉类和鱼类类似物,应该具有物理化学特性,如外观、感觉和味道,模仿它们所取代的动物性产品。这项研究的重点是利用先进的乳液技术开发植物性脂肪组织。使用由大豆油(60-85%)和大豆蛋白(2wt%)组装的水包油高内相乳液(HIPE)来模拟牛肉脂肪组织的光学和流变特性。含有75%或80%油的HIPE的微观结构和外观与牛肉脂肪组织相似。然而,脂肪组织中脂肪细胞的直径约为100µm,而HIPE中脂肪滴的直径仅为10µm左右。HIPE和脂肪组织的剪切流变学在高温(>;60°C)下相似,但牛肉脂肪在较低温度下更硬,这归因于脂肪结晶。HIPE的硬度随着脂肪含量从60%增加到80%而增加,但当其进一步提高到85%时,由于乳液分解而降低。这里开发的植物性脂肪组织可能有助于生产某些肉类或鱼类类似物。
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引用次数: 0
Effect of aqueous ethanol washing on functional properties of sunflower materials for meat analogue application 水乙醇洗涤对肉类模拟用向日葵材料功能性能的影响
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-07-01 DOI: 10.1016/j.foostr.2022.100274
Wanqing Jia , Ignatia Regina Sutanto , Mbalo Ndiaye , Julia K. Keppler , Atze Jan van der Goot

Sunflower kernel is rich in protein and is considered an alternative plant protein source for meat analogue application. The effect of process conditions used to make ingredients from sunflower kernel needs to be investigated on the functional and structuring properties. Pre-pressing and de-oiling with 96% ethanol were applied to obtain de-oiled sunflower kernel (DSK). Pressed sunflower kernel (PSK) was obtained by more intensive pressing only. Their concentrates were obtained by aqueous ethanol washing to study the effect of oil and phenol removal. DSK and its concentrate formed a fibrous product at 40 wt% by shearing and heating at 140 ℃ in a shear cell. The washed DSK led to stronger products upon shearing and heating. However, PSK, before and after washing only formed a crumble gel under the same conditions. The differences for the water holding capacity, nitrogen solubility index, and rheological properties were remarkably small between DSK and PSK, and the washed concentrates. The remaining oil in the sunflower material is crucial for the structuring potential, while the phenol content does not seem to have a relevant influence. The primary de-oiling process turned out to be a determining factor for further oil removal with aqueous ethanol washing.

向日葵仁富含蛋白质,被认为是肉类类似物应用的替代植物蛋白质来源。需要研究从葵花籽中制备成分的工艺条件对功能和结构性质的影响。采用预压和96%乙醇脱油的方法制备脱油向日葵仁(DSK)。压制的向日葵仁(PSK)是通过更密集的压制获得的。用乙醇水溶液洗涤得到它们的浓缩物,研究其除油和除酚效果。DSK及其浓缩物在剪切池中经140℃剪切加热,形成40wt%的纤维状产物。洗涤后的DSK在剪切和加热时产生更强的产品。然而,PSK在洗涤前后仅在相同条件下形成碎凝胶。DSK和PSK以及洗涤后的浓缩物在持水能力、氮溶解度指数和流变性能方面的差异非常小。向日葵材料中剩余的油对结构潜力至关重要,而苯酚含量似乎没有相关影响。一次除油过程被证明是用含水乙醇洗涤进一步除油的决定因素。
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引用次数: 0
Effect of Freeze-Thaw Cycles at Different Temperatures on the Properties of Gluten Proteins in Unfermented Dough 不同温度冻融循环对未发酵面团面筋蛋白性质的影响
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-05-18 DOI: 10.2139/ssrn.3883861
Yunfei Dai, H. Gao, Xiaoling Tian, Keqiang Huang, Yufen Liu, Jie Zeng, Mengyu Wang, Yueqi Qin
The effects of freeze-thaw cycles (from 1 to 5 cycles) at different temperatures (-6 °C, -12 °C, -18 °C, -24 °C, and -30 °C) on the properties of gluten isolated from dough were investigated. The results showed that after the same number of freeze-thaw cycle, the water-holding capacity of gluten protein was higher at lower temperatures, and the viscoelasticity and bound water content increased slightly with decreasing temperature. The microstructure of gluten protein was more uniform and relatively complete at -30 °C and -24 °C compared with other temperature conditions. Lower temperatures led to a significant decrease in the proportion of α-helices and β-turns and a notable increase in that of β-sheets. At the same temperature, the water-holding capacity, bound water content and viscoelasticity decreased with increasing number of freeze-thaw cycles. Compared with the first freeze-thaw cycle (F1), the cavity in the gluten microstructure of the fifth freeze-thaw cycle (F5) was larger and even exhibited fracture. In short, the increase in the number of freeze-thaw cycles damages the properties of gluten protein, and lower temperatures are more conducive to maintaining the stability of gluten properties.
研究了不同温度(-6°C、-12°C、-18°C、-24°C和-30°C)下的冻融循环(1-5个循环)对从面团中分离的面筋性能的影响。结果表明,经过相同次数的冻融循环后,面筋蛋白在较低温度下的持水能力较高,粘弹性和结合水含量随温度的降低略有增加。与其他温度条件相比,在-30°C和-24°C下,面筋蛋白的微观结构更加均匀且相对完整。较低的温度导致α-螺旋和β-转弯的比例显著降低,β-片的比例显著增加。在相同温度下,随着冻融循环次数的增加,保水能力、结合水含量和粘弹性均下降。与第一次冻融循环(F1)相比,第五次冻融周期(F5)的面筋微观结构中的空洞更大,甚至出现断裂。简而言之,冻融循环次数的增加损害了面筋蛋白的性质,而较低的温度更有利于保持面筋性质的稳定性。
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引用次数: 1
Editorial: special issue on Food Structure on Multiscale Simulations and Experimental Characterization of Foods 社论:《食品结构的多尺度模拟与实验表征》特刊
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-05-01 DOI: 10.1016/j.foostr.2022.100283
Ruud van der Sman, J. V. van Duynhoven
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引用次数: 1
Interaction between large deformation and moisture transport during dehydration of vegetables 蔬菜脱水过程中大变形与水分输送的相互作用
IF 4.7 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1016/j.foostr.2022.100269
Xin Jin , R.G.M. van der Sman

In this work, we present a multiphysics model describing heat and mass transport during drying of broccoli stalks, which is coupled to mechanical deformation. The model predicts collapse of pores due to compressive stresses during the initial stage of drying, which is later followed by reopening of pores if the liquid pressure gets under tension. This behaviour is qualitative in line with the experimental MRI observations during drying of broccoli stalk. The MRI observations show an apparent increase of moisture content in the centre of the sample during the initial stage of drying, which is consistent with the collapse of pores. In the later stages of drying, the MRI signal is prone to susceptibility effects, which can be explained by the reopened pores in the outer regions of the stalk. The purpose of this study is to show how mechanical deformation can be incorporated in a multiphysics model of drying of vegetables in a thermodynamic consistent manner.

在这项工作中,我们提出了一个多物理场模型,描述了西兰花茎干燥过程中的热量和质量传递,这与机械变形有关。该模型预测,在干燥的初始阶段,由于压缩应力,孔隙会崩溃,随后,如果液体压力受到拉紧,孔隙会重新打开。这种行为与西兰花茎干过程中的实验MRI观察结果定性一致。核磁共振成像观察显示,在干燥初期,样品中心的水分含量明显增加,这与孔隙的塌陷一致。在干燥后期,MRI信号容易受到磁化效应的影响,这可以解释为茎秆外部区域的气孔重新开放。本研究的目的是展示如何以热力学一致的方式将机械变形纳入蔬菜干燥的多物理场模型中。
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引用次数: 2
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Food Structure-Netherlands
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