{"title":"KA-2 Structural Analysis Based on Measurements of Slight Cation Displacements from High Resolution STEM Images","authors":"S. Kobayashi","doi":"10.1093/jmicro/dfz044","DOIUrl":"https://doi.org/10.1093/jmicro/dfz044","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46044511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Adachi, T. Ogawa, Zhi‐Lei Wang, Kohei Enya, Fumito Ajioka
{"title":"SM-6 Thorough quantification of micrograph by AI and advanced mathematics","authors":"Y. Adachi, T. Ogawa, Zhi‐Lei Wang, Kohei Enya, Fumito Ajioka","doi":"10.1093/jmicro/dfz059","DOIUrl":"https://doi.org/10.1093/jmicro/dfz059","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":"1 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60941208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SB-5 Abstraction of Pathological Information and Integration of Cancer Genome Information","authors":"S. Ishikawa","doi":"10.1093/jmicro/dfz051","DOIUrl":"https://doi.org/10.1093/jmicro/dfz051","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47691567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SS1-4 3D reconstruction of intracytoplasmic membrane structure of methane-oxidizing bacteria by electron microscopy imaging","authors":"K. Uematsu, Chong Chen, H. Hirayama","doi":"10.1093/jmicro/dfz065","DOIUrl":"https://doi.org/10.1093/jmicro/dfz065","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48077618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Tsukasaki, Tomoki Uchiyama, K. Yamamoto, Misae Otoyama, H. Kowada, Atsuki Atarashi, S. Mori, Y. Uchimoto, A. Hayashi, M. Tatsumisago
{"title":"PM-01 Exothermic behavior and microstructures of the LiNi1/3Mn1/3Co1/3O2 positive electrode layer for all-solid-state lithium batteries.","authors":"H. Tsukasaki, Tomoki Uchiyama, K. Yamamoto, Misae Otoyama, H. Kowada, Atsuki Atarashi, S. Mori, Y. Uchimoto, A. Hayashi, M. Tatsumisago","doi":"10.1093/jmicro/dfz101","DOIUrl":"https://doi.org/10.1093/jmicro/dfz101","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47564945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PB-15 Morphological Analysis of Reticular Dermis of Human Keloid Tissue","authors":"S. Ichinose, Chiemi Kaku, R. Ogawa","doi":"10.1093/jmicro/dfz072","DOIUrl":"https://doi.org/10.1093/jmicro/dfz072","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45923828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Yamaguchi, S. Wakabayashi, Y. Nakamura, H. Matsue, T. Hirao, Shigeki Aoki, H. Yamada, Nobuya Mamizu, H. Furukawa, H. Chibana
{"title":"PB-10 High-pressure freezing method can be replaced by sandwich freezing method !?: electron microscopy of human tissues and cultured cells","authors":"M. Yamaguchi, S. Wakabayashi, Y. Nakamura, H. Matsue, T. Hirao, Shigeki Aoki, H. Yamada, Nobuya Mamizu, H. Furukawa, H. Chibana","doi":"10.1093/jmicro/dfz085","DOIUrl":"https://doi.org/10.1093/jmicro/dfz085","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45824558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PM-18 Technology for fundamentally improving an extremely low-quality video signal used for fine focusing and astigmatism correction in scanning electron microscopy","authors":"Sadao Yamazaki, Kazuhiko Suzuki, E. Oho","doi":"10.1093/jmicro/dfz106","DOIUrl":"https://doi.org/10.1093/jmicro/dfz106","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46889037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Sharmin, K. Atsuzawa, Stephan Jung, S. Schubert, Y. Kaneko
Novel para-crystalline structures resembling prolamellar bodies in etioplasts were found in the invasion zones of indeterminate root nodules of Vicia faba, which possess persistent meristems and exhibit sequential developmental stages. The para-crystalline structures existed in most cells in the area of the invasion zone and a hexagonal arrangement of tubular membranes was recognized. Extensive membranes, apparently procured from the structures, were often in contact with the bacteria in young infected cells. We propose that the para-crystalline structures serve as a reservoir of membranes for the formation of the numerous symbiosomes that propagate and fill the infected cells, and suggest naming them pro-symbiosome membrane bodies.
{"title":"Para-crystalline membrane structures resembling prolamellar bodies in the invasion zones of indeterminate root nodules of Vicia faba L.","authors":"F. Sharmin, K. Atsuzawa, Stephan Jung, S. Schubert, Y. Kaneko","doi":"10.1093/jmicro/dfz027","DOIUrl":"https://doi.org/10.1093/jmicro/dfz027","url":null,"abstract":"Novel para-crystalline structures resembling prolamellar bodies in etioplasts were found in the invasion zones of indeterminate root nodules of Vicia faba, which possess persistent meristems and exhibit sequential developmental stages. The para-crystalline structures existed in most cells in the area of the invasion zone and a hexagonal arrangement of tubular membranes was recognized. Extensive membranes, apparently procured from the structures, were often in contact with the bacteria in young infected cells. We propose that the para-crystalline structures serve as a reservoir of membranes for the formation of the numerous symbiosomes that propagate and fill the infected cells, and suggest naming them pro-symbiosome membrane bodies.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42277528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Correlative light and electron microscopy (CLEM) enables ultrastructural-level analysis of fluorescence-labeled proteins by combining images obtained from both fluorescence and electron microscopies. A technical challenge with the CLEM method is the effective detection of fluorescence from samples embedded in resins, which generally cause fluorescence decay. To overcome this issue, we developed a method for fluorescence recovery of green fluorescent protein (GFP) in resin-embedded semi-thin sections using commercially available antifade reagents. By applying this method, we successfully obtained CLEM images using field-emission scanning electron microscopy with moderately enhanced GFP signals, demonstrating the efficacy of this simple fluorescence recovery method.
{"title":"Efficient fluorescence recovery using antifade reagents in correlative light and electron microscopy.","authors":"K. Toyooka, Naeko Shinozaki‐Narikawa","doi":"10.1093/jmicro/dfz029","DOIUrl":"https://doi.org/10.1093/jmicro/dfz029","url":null,"abstract":"Correlative light and electron microscopy (CLEM) enables ultrastructural-level analysis of fluorescence-labeled proteins by combining images obtained from both fluorescence and electron microscopies. A technical challenge with the CLEM method is the effective detection of fluorescence from samples embedded in resins, which generally cause fluorescence decay. To overcome this issue, we developed a method for fluorescence recovery of green fluorescent protein (GFP) in resin-embedded semi-thin sections using commercially available antifade reagents. By applying this method, we successfully obtained CLEM images using field-emission scanning electron microscopy with moderately enhanced GFP signals, demonstrating the efficacy of this simple fluorescence recovery method.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46279239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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