Pub Date : 2023-11-05DOI: 10.25303/1812rjbt1350141
Bi Maryam, Hoskeri H. Joy
Glucosinolates (GSL) are well-known secondary metabolites present in plants belonging to the Brassicaceae family (Cabbage, broccoli, cauliflower and brussels sprouts) and have been studied for their pharmacological properties. Glucosinolate is a sulfur-rich anionic hydrolyzed product which is good for the human diet. Through the enriched diet, the myrosinase enzyme hydrolyzes glucosinolates to form isothiocyanates against bacterial and fungal infections. Glucosinolates also show antimicrobial, anti-inflammatory, anticancer and cholinesterase inhibitor activity. The present study is to evaluate the glucosinolate substructures retrieved from the chemical database and to evaluate to understand that the BCL-2 inhibition showed the tumor growth and induced apoptosis. We retrieved 117 glucosinolate compounds and in silico druglike prediction screened the compounds and molecular docking against the BCL-2 protein structure. Based on the druglikeness analysis, we identified 39 compounds and these compounds were used for the inhibitory study with BCL2 protein. The results show that 2-Methyl-2-propenyl glucosinolate, 4-Methylpentyl glucosinolate, 4-Mercaptobutyl glucosinolate, Glucocapparin, Glucoerucin, Glucolepidiin, Glucoputranjivin, Glucoviorylin, n-Butyl glucosinolate compounds are strongly binding to BCL-2 protein structure by forming three hydrogen bonds and the resultant compounds can be used as a potential lead molecule for BCL-2 inhibitors.
{"title":"In silico studies on characterization of glucosinolate derivatives for inhibition ofcell cycle regulation protein BCL-2","authors":"Bi Maryam, Hoskeri H. Joy","doi":"10.25303/1812rjbt1350141","DOIUrl":"https://doi.org/10.25303/1812rjbt1350141","url":null,"abstract":"Glucosinolates (GSL) are well-known secondary metabolites present in plants belonging to the Brassicaceae family (Cabbage, broccoli, cauliflower and brussels sprouts) and have been studied for their pharmacological properties. Glucosinolate is a sulfur-rich anionic hydrolyzed product which is good for the human diet. Through the enriched diet, the myrosinase enzyme hydrolyzes glucosinolates to form isothiocyanates against bacterial and fungal infections. Glucosinolates also show antimicrobial, anti-inflammatory, anticancer and cholinesterase inhibitor activity. The present study is to evaluate the glucosinolate substructures retrieved from the chemical database and to evaluate to understand that the BCL-2 inhibition showed the tumor growth and induced apoptosis. We retrieved 117 glucosinolate compounds and in silico druglike prediction screened the compounds and molecular docking against the BCL-2 protein structure. Based on the druglikeness analysis, we identified 39 compounds and these compounds were used for the inhibitory study with BCL2 protein. The results show that 2-Methyl-2-propenyl glucosinolate, 4-Methylpentyl glucosinolate, 4-Mercaptobutyl glucosinolate, Glucocapparin, Glucoerucin, Glucolepidiin, Glucoputranjivin, Glucoviorylin, n-Butyl glucosinolate compounds are strongly binding to BCL-2 protein structure by forming three hydrogen bonds and the resultant compounds can be used as a potential lead molecule for BCL-2 inhibitors.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"55 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Ashwini, Vijith V. Shetty, A. Raghotham, Praveen Kumar Shetty, K. Suchetha
Glioblastomas (GBM) or grade IV astrocytomas is the most aggressive and frequent tumor in the Central Nervous System (CNS). GBM treatment mainly consists of surgical resection and radio and/or chemotherapy. However, due to its infiltration capacity, it is practically impossible to completely extract the tumor and it relapses. Aberrant EGFR expression is observed in GBM including EGFR mutation, rearrangement and deletions. Blood brain barrier is one of the major obstacles which prevents the entry of larger molecules into the brain. Hence a small molecule, with lesser neurotoxicity should be developed in treating GBM or any other brain related diseases. Previous studies have reported the therapeutic potency of 17-ꞵ Estradiol in treating breast cancer, but little is known about the therapeutic role of estrogen signalling in the development and progression of gliomas. Estradiol being the small molecule has molecular weight of 272.4g/mol. It is a lipophilic molecule and therefore it can pass through the blood brain barrier by passive diffusion and could be considered as a therapeutic molecule in treating GBM. Present study showed that 17-ꞵ Estradiol inhibited the migration, colony formation in GBM cell lines. Further it induced early and late apoptosis and arrested the cell cycle at S-Phase of the cell cycle. EGFR and downstream EGFR associated protein expression were also reduced upon exposure of GBM cell lines to E2. These results show that 17-ꞵ Estradiol could be considered as a therapeutic molecule in GBM treatment.
{"title":"Study on in vitro therapeutic impact of 17-ꞵ Estradiol on Glioblastoma cell lines","authors":"K. Ashwini, Vijith V. Shetty, A. Raghotham, Praveen Kumar Shetty, K. Suchetha","doi":"10.25303/1812rjbt0106","DOIUrl":"https://doi.org/10.25303/1812rjbt0106","url":null,"abstract":"Glioblastomas (GBM) or grade IV astrocytomas is the most aggressive and frequent tumor in the Central Nervous System (CNS). GBM treatment mainly consists of surgical resection and radio and/or chemotherapy. However, due to its infiltration capacity, it is practically impossible to completely extract the tumor and it relapses. Aberrant EGFR expression is observed in GBM including EGFR mutation, rearrangement and deletions. Blood brain barrier is one of the major obstacles which prevents the entry of larger molecules into the brain. Hence a small molecule, with lesser neurotoxicity should be developed in treating GBM or any other brain related diseases. Previous studies have reported the therapeutic potency of 17-ꞵ Estradiol in treating breast cancer, but little is known about the therapeutic role of estrogen signalling in the development and progression of gliomas. Estradiol being the small molecule has molecular weight of 272.4g/mol. It is a lipophilic molecule and therefore it can pass through the blood brain barrier by passive diffusion and could be considered as a therapeutic molecule in treating GBM. Present study showed that 17-ꞵ Estradiol inhibited the migration, colony formation in GBM cell lines. Further it induced early and late apoptosis and arrested the cell cycle at S-Phase of the cell cycle. EGFR and downstream EGFR associated protein expression were also reduced upon exposure of GBM cell lines to E2. These results show that 17-ꞵ Estradiol could be considered as a therapeutic molecule in GBM treatment.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.25303/1812rjbt1470150
S. Narasimhan, S. Bindu
The magnetic field can affect the structure, physiology and biochemistry of plants. The use of magnetic fields on cultured plant cells and tissues is a proven fact. The system to deliver magnetic flux is very important. Experiments have been performed by directly placing cultures in the vicinity of the magnetic field from a permanent magnetic source. Such a system delivered a fixed magnetic strength. Electric magnets are a better mode to deliver magnetic field to plants because it is easy to deliver tailor-made magnetic exposure. Majority of such studies utilized a magnetic field in the order of milliteslas, some of them utilized a high magnetic field upto 17.8 Tesla. The experiments also utilized varying exposure times and differential exposure to the north or south pole. Such studies reported either an increase or decrease of many parameters such as cell size, shape, metabolite content, chlorophyll content and enzyme activity. Most of the studies discussing magnetotropism on plants utilized seeds or intact plants to find out the effect of magnetic fields on plants. Such studies also give some understanding of the mechanism of action. Using a plant cell culture system, it is possible to grow single cells and small cell aggregates. Therefore, in vitro systems offer an excellent tool to understand the mechanism of action at the cellular level.
{"title":"An assessment of the application of magnetic fields in the study of in vitro plant cell and tissue cultivation","authors":"S. Narasimhan, S. Bindu","doi":"10.25303/1812rjbt1470150","DOIUrl":"https://doi.org/10.25303/1812rjbt1470150","url":null,"abstract":"The magnetic field can affect the structure, physiology and biochemistry of plants. The use of magnetic fields on cultured plant cells and tissues is a proven fact. The system to deliver magnetic flux is very important. Experiments have been performed by directly placing cultures in the vicinity of the magnetic field from a permanent magnetic source. Such a system delivered a fixed magnetic strength. Electric magnets are a better mode to deliver magnetic field to plants because it is easy to deliver tailor-made magnetic exposure. Majority of such studies utilized a magnetic field in the order of milliteslas, some of them utilized a high magnetic field upto 17.8 Tesla. The experiments also utilized varying exposure times and differential exposure to the north or south pole. Such studies reported either an increase or decrease of many parameters such as cell size, shape, metabolite content, chlorophyll content and enzyme activity. Most of the studies discussing magnetotropism on plants utilized seeds or intact plants to find out the effect of magnetic fields on plants. Such studies also give some understanding of the mechanism of action. Using a plant cell culture system, it is possible to grow single cells and small cell aggregates. Therefore, in vitro systems offer an excellent tool to understand the mechanism of action at the cellular level.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sudip Mondal, Rituparna Saha, Bijoy Mal, K. Acharya, Gunjan Biswas
Mushrooms are well-known pharmaceutical agents as they possess bioactive secondary metabolites along with a large amount of proteins, carbohydrates, crude fiber, fat and other beneficial compounds. Due to their remarkable pharmacological property, they are used in traditional medicine in different countries of Asia. Pycnoporus sanguineus is a polypore fungus having a wide variety of bioactive substances and therapeutic activities that are reported previously in different countries of the world. The present study is focused to estimate different bioactive compounds and evaluate antioxidant and anti-inflammatory activities of P. sanguineus using two different extraction procedures (infusion and decoction). In this study, total phenol, total flavonoid, β-carotene, lycopene and ascorbic acid contents are estimated. Antioxidant and inti-inflammatory activities are investigated by total antioxidant capacity, DPPH radical scavenging, ABTS radical scavenging, chelating of ferrous ion (Fe2+), reducing power assays and protein denaturation assay respectively. The aim of the study is to establish P. sanguineus as a natural drug that could be used against oxidative stress and inflammation-related diseases substituting commercial drugs.
{"title":"Comparative study of phytochemicals, antioxidant and anti-inflammatory activity of infusion and decoction extracts from Pycnoporus sanguineus (L.) Murril collected in West Bengal","authors":"Sudip Mondal, Rituparna Saha, Bijoy Mal, K. Acharya, Gunjan Biswas","doi":"10.25303/1812rjbt07013","DOIUrl":"https://doi.org/10.25303/1812rjbt07013","url":null,"abstract":"Mushrooms are well-known pharmaceutical agents as they possess bioactive secondary metabolites along with a large amount of proteins, carbohydrates, crude fiber, fat and other beneficial compounds. Due to their remarkable pharmacological property, they are used in traditional medicine in different countries of Asia. Pycnoporus sanguineus is a polypore fungus having a wide variety of bioactive substances and therapeutic activities that are reported previously in different countries of the world. The present study is focused to estimate different bioactive compounds and evaluate antioxidant and anti-inflammatory activities of P. sanguineus using two different extraction procedures (infusion and decoction). In this study, total phenol, total flavonoid, β-carotene, lycopene and ascorbic acid contents are estimated. Antioxidant and inti-inflammatory activities are investigated by total antioxidant capacity, DPPH radical scavenging, ABTS radical scavenging, chelating of ferrous ion (Fe2+), reducing power assays and protein denaturation assay respectively. The aim of the study is to establish P. sanguineus as a natural drug that could be used against oxidative stress and inflammation-related diseases substituting commercial drugs.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"76 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.25303/1812rjbt1510167
S. Chhotaray, S. Jal
Biosensors are target-specific affinity based analytical tools. Their applications are vast in areas such as clinical diagnosis, food quality, environment monitoring and in other fields as well where timely and reliable analysis are required. Biosensing technology is gradually developing to produce nove.l biosensors for point-of-care diagnosis. The involvement of new advancements such as aptamer-based technologies and nano-bioelectronic technologies have great potentialto lead to the development of next generation biosensors with improved sensitivity, specificity, portability and cost effectiveness. This review emphasizes several aspects of biosensors such as their classification, mechanism involved and their biomedical applications.
{"title":"Biosensors from the Perspective of Sensing Labelled and Unlabelled Targets","authors":"S. Chhotaray, S. Jal","doi":"10.25303/1812rjbt1510167","DOIUrl":"https://doi.org/10.25303/1812rjbt1510167","url":null,"abstract":"Biosensors are target-specific affinity based analytical tools. Their applications are vast in areas such as clinical diagnosis, food quality, environment monitoring and in other fields as well where timely and reliable analysis are required. Biosensing technology is gradually developing to produce nove.l biosensors for point-of-care diagnosis. The involvement of new advancements such as aptamer-based technologies and nano-bioelectronic technologies have great potentialto lead to the development of next generation biosensors with improved sensitivity, specificity, portability and cost effectiveness. This review emphasizes several aspects of biosensors such as their classification, mechanism involved and their biomedical applications.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"173 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.25303/1812rjbt1140121
C. Shaji, Radha Saraswathy
The global prevalence of Type 2 diabetes mellitus (T2DM) has been observed to escalate annually. However, studies also report a higher prevalence of undiagnosed diabetes. This results in disease progression accompanied with development of macro and micro complications. Taste disorders are significant clinical manifestations for diabetes which have been ignored in majority of the cases. Certain trace elements are identified to be associated with the development and progression of diabetes and are also established to activate or suppress the functioning of human taste receptors. This study aims at determining the serum trace element concentrations in diabetic (n =153) and non-diabetic (n=153) subjects who have been screened for taste perception anomalies. Linear correlation of trace metal levels with glucose levels showed a slightly positive association of Cu, Cr and Cd with FBG. Multiple logistic regression model predicted Cu levels, Cd toxicity and taste anomaly as significant biomarkers for T2DM. This would enable early diagnosis of T2DM, thus facilitating appropriate medical intervention to avoid multiple complications in T2DM individuals.
{"title":"Copper levels, Cadmium toxicity and presence of Taste anomalies as predictors for early diagnosis of Type 2 Diabetes Mellitus","authors":"C. Shaji, Radha Saraswathy","doi":"10.25303/1812rjbt1140121","DOIUrl":"https://doi.org/10.25303/1812rjbt1140121","url":null,"abstract":"The global prevalence of Type 2 diabetes mellitus (T2DM) has been observed to escalate annually. However, studies also report a higher prevalence of undiagnosed diabetes. This results in disease progression accompanied with development of macro and micro complications. Taste disorders are significant clinical manifestations for diabetes which have been ignored in majority of the cases. Certain trace elements are identified to be associated with the development and progression of diabetes and are also established to activate or suppress the functioning of human taste receptors. This study aims at determining the serum trace element concentrations in diabetic (n =153) and non-diabetic (n=153) subjects who have been screened for taste perception anomalies. Linear correlation of trace metal levels with glucose levels showed a slightly positive association of Cu, Cr and Cd with FBG. Multiple logistic regression model predicted Cu levels, Cd toxicity and taste anomaly as significant biomarkers for T2DM. This would enable early diagnosis of T2DM, thus facilitating appropriate medical intervention to avoid multiple complications in T2DM individuals.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"227 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.25303/1812rjbt1300134
Pradeep Choudhary, Vishal Khandelwal
Limited study has been done so far to investigate immunomodulatory efficacy of whole plant of hot aqueous extract of Solanum xanthocarpum (HAESX) in Wistar albino rats. Present work has been designed to study immunomodulatory effect of HAESX in Wistar albino rat with respect to splenocyte proliferation and cytokines induction. Concanavalin A (Con A) activated splenocytes were used to measure in vitro cell mediated immune response using 3-(4, 5-Dimethylthiazolyl)-2, 5-diphenyl tetrazolium bromide (MTT) in the presence of varied doses of HAESX (20-500 μg/mL). The effects of HAESX on cell mediated immune response were studied using six groups of Wistar albino rats. Tumor necrosis factor-α (TNF-α), Interferon-γ (IFN-γ), Interleukin-10 (IL-10) and Interleukin-4 (IL-4) were measured in splenocyte culture supernatant by sandwich ELISA with different concentrations of HAESX (20-500 μg/mL). Study depicts significant decrease (p<0.05) in the proliferation of Con A treated splenocyte at 250 and 500 μg/mL HAESX, reflecting cell mediated immunoinhibitory potential of HAESX. Dose dependent significant increase (p<0.01) in the amount of IL-10 was found in splenocyte culture supernatant with increase in HAESX concentrations. TNF-α, IFN-γ and IL-4 were found to decrease significantly (p<0.01) with increase in HAESX concentrations. Study revealed that HAESX of Solanum xanthocarpum is found to influence the networking of cytokines, implying that it may play a function in regulating the activity of the host's immune system and can serve as potent herbal drug against immune rejection.
{"title":"Immunomodulatory potential of aqueous extract of Solanum xanthocarpum with reference to splenocyte proliferation and cytokines induction in Wistar albino rats","authors":"Pradeep Choudhary, Vishal Khandelwal","doi":"10.25303/1812rjbt1300134","DOIUrl":"https://doi.org/10.25303/1812rjbt1300134","url":null,"abstract":"Limited study has been done so far to investigate immunomodulatory efficacy of whole plant of hot aqueous extract of Solanum xanthocarpum (HAESX) in Wistar albino rats. Present work has been designed to study immunomodulatory effect of HAESX in Wistar albino rat with respect to splenocyte proliferation and cytokines induction. Concanavalin A (Con A) activated splenocytes were used to measure in vitro cell mediated immune response using 3-(4, 5-Dimethylthiazolyl)-2, 5-diphenyl tetrazolium bromide (MTT) in the presence of varied doses of HAESX (20-500 μg/mL). The effects of HAESX on cell mediated immune response were studied using six groups of Wistar albino rats. Tumor necrosis factor-α (TNF-α), Interferon-γ (IFN-γ), Interleukin-10 (IL-10) and Interleukin-4 (IL-4) were measured in splenocyte culture supernatant by sandwich ELISA with different concentrations of HAESX (20-500 μg/mL). Study depicts significant decrease (p<0.05) in the proliferation of Con A treated splenocyte at 250 and 500 μg/mL HAESX, reflecting cell mediated immunoinhibitory potential of HAESX. Dose dependent significant increase (p<0.01) in the amount of IL-10 was found in splenocyte culture supernatant with increase in HAESX concentrations. TNF-α, IFN-γ and IL-4 were found to decrease significantly (p<0.01) with increase in HAESX concentrations. Study revealed that HAESX of Solanum xanthocarpum is found to influence the networking of cytokines, implying that it may play a function in regulating the activity of the host's immune system and can serve as potent herbal drug against immune rejection.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrographis paniculata is one of the most important medicinal plants having several pharmaceutical properties. Various pharmaceutical products and raw powders of this herb are sold in the market. The high demand for A. paniculata poses the problem of adulteration in their products. Therefore, it becomes necessary to check the quality in terms of their major bioactive compounds. In the present study, several marketed products were analyzed for their quality assessment i.e. for the presence of desired bioactive constituent, andrographolide. Ten tested products of A. paniculata showed the presence of andrographolide. A validated High Performance Thin Layer Chromatography (HPLC) method has been used for the quantitative estimation of andrographolide. andrographolide was detected at a retention time (Rt) of 17.8±0.05 and was quantified using a linear regression equation. The amount of andrographolide in the marketed products was in the range of 0.01 to 7.08 %. The chromatographic fingerprints were developed in all marketed products in the study. Thus, the present study dealing with the detection and quantification of andrographolide through HPLC analysis would have enough significance in the quality control of marketed products of andrographis paniculata.
穿心莲是最重要的药用植物之一，具有多种药用功效。市场上出售各种药用产品和这种草药的原粉。穿心莲的高需求量带来了产品掺假的问题。因此，有必要检查其主要生物活性化合物的质量。在本研究中，对几种市场上销售的产品进行了质量评估分析，即是否含有所需的生物活性成分穿心莲内酯。经检测，10 种 A. paniculata 产品含有穿心莲内酯。采用经过验证的高效薄层色谱法（HPLC）对穿心莲内酯进行定量估算。穿心莲内酯的保留时间（Rt）为 17.8±0.05，采用线性回归方程进行定量。市售产品中穿心莲内酯的含量范围为 0.01%至 7.08%。研究中的所有市售产品都形成了色谱指纹图谱。因此，本研究通过高效液相色谱分析对穿心莲内酯进行检测和定量，对穿心莲市场产品的质量控制具有重要意义。
{"title":"Quality control of marketed products of Andrographis paniculata by HPLC analysis","authors":"B. Champati, A. Ray, S. Jena, A. Sahoo, S. Nayak","doi":"10.25303/1812rjbt067070","DOIUrl":"https://doi.org/10.25303/1812rjbt067070","url":null,"abstract":"Andrographis paniculata is one of the most important medicinal plants having several pharmaceutical properties. Various pharmaceutical products and raw powders of this herb are sold in the market. The high demand for A. paniculata poses the problem of adulteration in their products. Therefore, it becomes necessary to check the quality in terms of their major bioactive compounds. In the present study, several marketed products were analyzed for their quality assessment i.e. for the presence of desired bioactive constituent, andrographolide. Ten tested products of A. paniculata showed the presence of andrographolide. A validated High Performance Thin Layer Chromatography (HPLC) method has been used for the quantitative estimation of andrographolide. andrographolide was detected at a retention time (Rt) of 17.8±0.05 and was quantified using a linear regression equation. The amount of andrographolide in the marketed products was in the range of 0.01 to 7.08 %. The chromatographic fingerprints were developed in all marketed products in the study. Thus, the present study dealing with the detection and quantification of andrographolide through HPLC analysis would have enough significance in the quality control of marketed products of andrographis paniculata.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"92 11 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anupama Moirangthem, T. Baruah, Anand Prakash Maurya, A. Patar, Susmita Paul, Birson Ingti
The present study aims to identify potential medicinal plant-based extended-spectrum β-lactamase (ESBL) inhibitors from Andrographis paniculata using both in silico and in vitro approaches. The ESBLs were obtained from the protein data bank. The structures of phytoconstituents were obtained from the PubChem database. The compounds were docked against different ESBLs (targeted proteins) using AutodockTools followed by molecular dynamics simulation. In silico results were further validated using in vitro testing through the disc diffusion method. The molecular docking revealed that most of the phytoconstituents have a good binding affinity. The binding energy and in vitro study of the phytoconstituents of Andrographis paniculata were compared with the standard inhibitor for ESBL i.e. clavulanic acid .14- Acetylandrographolide (AAD) showed good binding with the ESBL proteins, having the best values reported in the docking with OXA-10. Simulation of the complex of AAD and OXA-10 showed that the complex was relatively steady as evidenced by the lack of sudden fluctuations in the values of root mean square deviations, the radius of gyration and solvent-accessible surface area. Further confirmation of the in silico approach was done by an in vitro study against ESBL-producing organisms which showed inhibitory results. From this study, we can conclude that A.paniculata may have the potential to inhibit ESBLs and may be considered for treating bacterial infections.
{"title":"Identification of potential inhibitors from Andrographis paniculata bioactive compounds against extended-spectrum β-lactamases through in silico and in vitro approaches","authors":"Anupama Moirangthem, T. Baruah, Anand Prakash Maurya, A. Patar, Susmita Paul, Birson Ingti","doi":"10.25303/1812rjbt080090","DOIUrl":"https://doi.org/10.25303/1812rjbt080090","url":null,"abstract":"The present study aims to identify potential medicinal plant-based extended-spectrum β-lactamase (ESBL) inhibitors from Andrographis paniculata using both in silico and in vitro approaches. The ESBLs were obtained from the protein data bank. The structures of phytoconstituents were obtained from the PubChem database. The compounds were docked against different ESBLs (targeted proteins) using AutodockTools followed by molecular dynamics simulation. In silico results were further validated using in vitro testing through the disc diffusion method. The molecular docking revealed that most of the phytoconstituents have a good binding affinity. The binding energy and in vitro study of the phytoconstituents of Andrographis paniculata were compared with the standard inhibitor for ESBL i.e. clavulanic acid .14- Acetylandrographolide (AAD) showed good binding with the ESBL proteins, having the best values reported in the docking with OXA-10. Simulation of the complex of AAD and OXA-10 showed that the complex was relatively steady as evidenced by the lack of sudden fluctuations in the values of root mean square deviations, the radius of gyration and solvent-accessible surface area. Further confirmation of the in silico approach was done by an in vitro study against ESBL-producing organisms which showed inhibitory results. From this study, we can conclude that A.paniculata may have the potential to inhibit ESBLs and may be considered for treating bacterial infections.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"163 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To know the potential of flower waste as a substrate for biosurfactant production, strains of pure isolated fungi F1, F2, F3, F4 and F5 were used for the study. Different screening methods were done to test the production of biosurfactant: Emulsification Index Test, Drop Collapse Test, Oil Spreading Test, CTAB Agar Test and Hemolytic Activity Test. The highest percentage of biosurfactant concentration for Emulsification Index Test i.e. E24 value was shown by F1 and F3 with 54.16%. The highest clearing zone for Oil Spreading Test was shown by F1 and F3 strain with 2.2 cm diameter. The Drop Collapse Test was shown positive by F1 and F3 strain with flattening of 1mm as compared with distilled water. The clearing zone around the wells for hemolytic activity was shown by both F1 and F3 strain. CTAB Agar Test showed that out of the five fungal strains, F1 and F3 showed blue halos around the wells and hence are anionic in nature. Thus, flower waste has the potential for the production of biosurfactant as a low-cost substrate which can yield high value biosurfactant.
{"title":"Flower waste as a potential substrate for biosurfactant production","authors":"Julious Seikh, Jonali Owary","doi":"10.25303/1812rjbt071079","DOIUrl":"https://doi.org/10.25303/1812rjbt071079","url":null,"abstract":"To know the potential of flower waste as a substrate for biosurfactant production, strains of pure isolated fungi F1, F2, F3, F4 and F5 were used for the study. Different screening methods were done to test the production of biosurfactant: Emulsification Index Test, Drop Collapse Test, Oil Spreading Test, CTAB Agar Test and Hemolytic Activity Test. The highest percentage of biosurfactant concentration for Emulsification Index Test i.e. E24 value was shown by F1 and F3 with 54.16%. The highest clearing zone for Oil Spreading Test was shown by F1 and F3 strain with 2.2 cm diameter. The Drop Collapse Test was shown positive by F1 and F3 strain with flattening of 1mm as compared with distilled water. The clearing zone around the wells for hemolytic activity was shown by both F1 and F3 strain. CTAB Agar Test showed that out of the five fungal strains, F1 and F3 showed blue halos around the wells and hence are anionic in nature. Thus, flower waste has the potential for the production of biosurfactant as a low-cost substrate which can yield high value biosurfactant.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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