The use of heavy metal resistant plant growth promoting endophytes is an effective method for improving crop yield and cleaning up contaminated sites. In our study, we have isolated thirteen bacterial endophytes from the shoots of Alternanthera philoxeroides, an aquatic plant from Bellandur lake, Bangalore, India. Among the isolates, Bacillus paramycoides showed significant plant growth promotion properties including an extortionate amount of indole acetic acid (IAA) production (144.69 ± 1.01 μg/mL) along with other plant growth promoting attributes like ammonia production, nitrogen fixation, phosphate, potassium solubilization, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and siderophore production. The isolate also demonstrated the ability to resist pathogen attacks by producing extracellular enzymes, which could have potential industrial uses. Furthermore, it displayed resistance to multiple heavy metals like chromium (Cr), copper (Cu), lead (Pb), zinc (Zn) and cadmium (Cd) as well as the ability to tolerate high salt concentrations (up to 7% NaCl). These characteristics make it an ideal candidate for promoting plant growth in stressful environments and as an effective bioremediation agent.
{"title":"Unveiling the Potential of Bacillus paramycoides, a Halotolerant Endophytic Bacterium with Heavy Metal Tolerance and Plant Growth Promotion Properties","authors":"Indhu Philip, Suma Sarojini, Soma Biswas, Saranya Jayaram","doi":"10.25303/1903rjbt1200126","DOIUrl":"https://doi.org/10.25303/1903rjbt1200126","url":null,"abstract":"The use of heavy metal resistant plant growth promoting endophytes is an effective method for improving crop yield and cleaning up contaminated sites. In our study, we have isolated thirteen bacterial endophytes from the shoots of Alternanthera philoxeroides, an aquatic plant from Bellandur lake, Bangalore, India. Among the isolates, Bacillus paramycoides showed significant plant growth promotion properties including an extortionate amount of indole acetic acid (IAA) production (144.69 ± 1.01 μg/mL) along with other plant growth promoting attributes like ammonia production, nitrogen fixation, phosphate, potassium solubilization, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and siderophore production. The isolate also demonstrated the ability to resist pathogen attacks by producing extracellular enzymes, which could have potential industrial uses. Furthermore, it displayed resistance to multiple heavy metals like chromium (Cr), copper (Cu), lead (Pb), zinc (Zn) and cadmium (Cd) as well as the ability to tolerate high salt concentrations (up to 7% NaCl). These characteristics make it an ideal candidate for promoting plant growth in stressful environments and as an effective bioremediation agent.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140477195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ramasamy Srinivasan, D. Kamalanathan, Selvakumar Boobalan, Mani Karthikeyan
Transformed root culture in Aerva javanica was investigated for amassing of alkaloids. Leaves of A. javanica were used as establishing material for induction of hairy roots via the Agrobacterium rhizogenes A4 strain. Three hairy root lines from leaf explants upon growth kinetics were attained. Maceration method was used for preparation of different extracts viz. chloroform, petroleum ether, ethyl acetate, acetone and methanol. The extracts were examined against human bacterial pathogens by agar well diffusion method. According to our results, methanol extracts of hairy roots showed a significant antimicrobial activity against the tested pathogens particularly Pseudomonas aerogenes (19.29 ± 0.23mm) followed by Bacillus subtilis (18.49 ± 0.43mm) and Staphylococcus epidermidis (18.49 ± 0.68mm) rather than wild root extract. Methanol extracts of hairy root sample inhibited pathogen development better than other extracts and also than wild root extracts. Phytochemical analysis exhibited the presence of alkaloids, flavonoids, steroids, tannins, sugars and glycosides found only in methanol extract of A. javanica hairy roots. The results revealed that methanol extract of A. javanica hairy roots has potential activity than the other extracts as well as standard drugs (Spreptomycin). Further studies are needed related to pharmacological activities.
{"title":"Comparative phytochemical analysis and antimicrobial activity of methanol extracts of Hairy roots and wild roots from Aerva javanica (Burm.f) Juss. ex Schult against selected pathogenic microorganisms","authors":"Ramasamy Srinivasan, D. Kamalanathan, Selvakumar Boobalan, Mani Karthikeyan","doi":"10.25303/1903rjbt013019","DOIUrl":"https://doi.org/10.25303/1903rjbt013019","url":null,"abstract":"Transformed root culture in Aerva javanica was investigated for amassing of alkaloids. Leaves of A. javanica were used as establishing material for induction of hairy roots via the Agrobacterium rhizogenes A4 strain. Three hairy root lines from leaf explants upon growth kinetics were attained. Maceration method was used for preparation of different extracts viz. chloroform, petroleum ether, ethyl acetate, acetone and methanol. The extracts were examined against human bacterial pathogens by agar well diffusion method. According to our results, methanol extracts of hairy roots showed a significant antimicrobial activity against the tested pathogens particularly Pseudomonas aerogenes (19.29 ± 0.23mm) followed by Bacillus subtilis (18.49 ± 0.43mm) and Staphylococcus epidermidis (18.49 ± 0.68mm) rather than wild root extract. Methanol extracts of hairy root sample inhibited pathogen development better than other extracts and also than wild root extracts. Phytochemical analysis exhibited the presence of alkaloids, flavonoids, steroids, tannins, sugars and glycosides found only in methanol extract of A. javanica hairy roots. The results revealed that methanol extract of A. javanica hairy roots has potential activity than the other extracts as well as standard drugs (Spreptomycin). Further studies are needed related to pharmacological activities.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140475178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-31DOI: 10.25303/1903rjbt1350141
K. Sharanya, A.K.A. Mandal
In the contemporary world, lung cancer has become the most frequent type of cancer in humankind. Recent studies revealed that lung cancer proliferation is correlated with microRNA (miRNA) dysregulation. Several studies have identified PI3K/AKT signaling pathway as the key target of certain miRNAs and play a fundamental role in cellular activities involved in lung cancer cell invasion and metastasis. For lung cancer research, miRNAs and their interrelated target genes could be a capable novel field in its diagnosis as well as in therapy. This review highlights the role of regulatory miRNAs targeting the PI3K/AKT signaling pathway in lung carcinogenesis.
{"title":"Regulatory effects of miRNAs targeting PI3K/AKT signaling pathway in the pathogenesis of lung cancer","authors":"K. Sharanya, A.K.A. Mandal","doi":"10.25303/1903rjbt1350141","DOIUrl":"https://doi.org/10.25303/1903rjbt1350141","url":null,"abstract":"In the contemporary world, lung cancer has become the most frequent type of cancer in humankind. Recent studies revealed that lung cancer proliferation is correlated with microRNA (miRNA) dysregulation. Several studies have identified PI3K/AKT signaling pathway as the key target of certain miRNAs and play a fundamental role in cellular activities involved in lung cancer cell invasion and metastasis. For lung cancer research, miRNAs and their interrelated target genes could be a capable novel field in its diagnosis as well as in therapy. This review highlights the role of regulatory miRNAs targeting the PI3K/AKT signaling pathway in lung carcinogenesis.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140470943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rhizospheric soil samples of Sugarcane fields were collected from distinct regions of U.P. Further, these soil samples were used for the isolation and characterization of bacteria to identify their maximum PGPR traits and biochemical potential. A total of ten bacterial isolates were collected from the soil samples and sub-cultured in the nutrient agar plates and characterized through Biochemical test. One bacterial strain was found negative and the other 9 isolates were found positive for gram staining. All bacterial isolates were positive for citrate test. 3 isolates were found MR positive and 2 positives for VP test. 5 isolates were positive for gelatinase test and 9 isolates were positive for nitrate reductase test. 2 isolates were positive for starch hydrolysis test, while 7 isolates were found positive for phosphate solubilization test and 3 isolates were positive for siderophore production test. These results showed that the bacterial isolates from soil sample were Azotobacter Species, Azospirillum Species, Pseudomonas species, Bacillus Species, Rhizobium Species, Serratia Species and E. coli. These bacterial strains followed the maximum PGPR traits. After proper validation, these bacterial strains could be used for the bio-fertilizer formation for crop production enhancement by improving soil fertility.
{"title":"Biochemical characterization of bacteria isolated from Rhizosphere soils of sugarcane grown in Uttar Pradesh","authors":"N.S. Yadav, Sakshi Issar, R.K. Gaur","doi":"10.25303/1903rjbt070075","DOIUrl":"https://doi.org/10.25303/1903rjbt070075","url":null,"abstract":"Rhizospheric soil samples of Sugarcane fields were collected from distinct regions of U.P. Further, these soil samples were used for the isolation and characterization of bacteria to identify their maximum PGPR traits and biochemical potential. A total of ten bacterial isolates were collected from the soil samples and sub-cultured in the nutrient agar plates and characterized through Biochemical test. One bacterial strain was found negative and the other 9 isolates were found positive for gram staining. All bacterial isolates were positive for citrate test. 3 isolates were found MR positive and 2 positives for VP test. 5 isolates were positive for gelatinase test and 9 isolates were positive for nitrate reductase test. 2 isolates were positive for starch hydrolysis test, while 7 isolates were found positive for phosphate solubilization test and 3 isolates were positive for siderophore production test. These results showed that the bacterial isolates from soil sample were Azotobacter Species, Azospirillum Species, Pseudomonas species, Bacillus Species, Rhizobium Species, Serratia Species and E. coli. These bacterial strains followed the maximum PGPR traits. After proper validation, these bacterial strains could be used for the bio-fertilizer formation for crop production enhancement by improving soil fertility.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140470510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-31DOI: 10.25303/1903rjbt1270134
Mahasweta Guha
Silvalis jairajpurii sp. n., a new dorylaimid nematode under the subfamily Thornenematinae is described and illustrated in this study. This is found from rhizospheric soil of paddy in Gosaba block of Sunderban region of South 24 Parganas District of West Bengal, India. The species is characterised by medium sized body (1.5-1.6 mm) with sclerotized cheliorhabdions encircling the strong odontostyle, amphidelphic reproductive system with a very minute vaginal opening terminated by a short blunt, but rounded tail which show marked differences from other species of the genus. This is a plant parasite that affects paddy cultivation and rice in our staple food. So, this type of plant parasite causes economic loss of our country. The species key of this genus with compandium is also provided. Mylodiscus nanus is also rare nematode, reported from paddy field rhizospheric zone of Gosaba block, Sundarbans part of South 24 Parganas, West Bengal of India. It is a predatory nematode.
{"title":"Silvalis jairajpurii sp.n. with diagonostic compendium and keys to species including one known rare soil nematode (Nematoda: Dorylaimida) from Sundarban region of South 24 Parganas district, West Bengal, India","authors":"Mahasweta Guha","doi":"10.25303/1903rjbt1270134","DOIUrl":"https://doi.org/10.25303/1903rjbt1270134","url":null,"abstract":"Silvalis jairajpurii sp. n., a new dorylaimid nematode under the subfamily Thornenematinae is described and illustrated in this study. This is found from rhizospheric soil of paddy in Gosaba block of Sunderban region of South 24 Parganas District of West Bengal, India. The species is characterised by medium sized body (1.5-1.6 mm) with sclerotized cheliorhabdions encircling the strong odontostyle, amphidelphic reproductive system with a very minute vaginal opening terminated by a short blunt, but rounded tail which show marked differences from other species of the genus. This is a plant parasite that affects paddy cultivation and rice in our staple food. So, this type of plant parasite causes economic loss of our country. The species key of this genus with compandium is also provided. Mylodiscus nanus is also rare nematode, reported from paddy field rhizospheric zone of Gosaba block, Sundarbans part of South 24 Parganas, West Bengal of India. It is a predatory nematode.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140476925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-31DOI: 10.25303/1903rjbt1010107
Putu Ijiya Danta Awatara, Edvin Prawira Negara, Dewi Indiastari, Siwipeni Irmawanti Rahayu, Indriati Dwi Rahayu
The most common cause of nosocomial infection worldwide is caused by Gram-positive coccus bacteria or what we commonly call Methicillin-resistant Staphylococcus aureus (MRSA). This study aimed to find novel antimicrobial agents from the banyan tree (Ficus benjamina L.) hanging roots to treat MRSA. Docking experiment and well diffusion were used as in silico and in vitro experiments respectively to identify the potential of stigmasterol against MRSA. The data were analyzed statistically with one-way variance analysis (ANOVA), post-doc Tukey assay, correlation, and regression by SPSS Amos 24. In the in silico experiment, stigmasterol was strongly associated with penicillin-binding protein 2a (PBP2a) on the active side of LYS289. Thus, it had the potential to inhibit PBP2a. Disruption of cell walls, the release of cytoplasmic contents, and a decrease in the cellular volume of MRSA were caused by the inhibition of PBP2a. The well diffusion test results showed the inhibition zone in 50 - 100 μg/ml of stigmasterol against MRSA. The inhibition zone was in the range of 7.38 – 10.17 mm. The inhibition diameter indicated a significant difference with a p-value < 0.05. The results of statistical analyses indicate that stigmasterol is effective as an antimicrobial against MRSA. Stigmasterol has potential as a potential microbial agent for the therapy of MRSA.
{"title":"In vitro and in silico Studies: Penicillin-Binding Protein 2a (PBP2a) Inhibition by Stigmasterol in Banyan Hanging Roots (Ficus benjamina L.) as Novel Treatment of Methicillin-Resistant Staphylococcus aureus (MRSA)","authors":"Putu Ijiya Danta Awatara, Edvin Prawira Negara, Dewi Indiastari, Siwipeni Irmawanti Rahayu, Indriati Dwi Rahayu","doi":"10.25303/1903rjbt1010107","DOIUrl":"https://doi.org/10.25303/1903rjbt1010107","url":null,"abstract":"The most common cause of nosocomial infection worldwide is caused by Gram-positive coccus bacteria or what we commonly call Methicillin-resistant Staphylococcus aureus (MRSA). This study aimed to find novel antimicrobial agents from the banyan tree (Ficus benjamina L.) hanging roots to treat MRSA. Docking experiment and well diffusion were used as in silico and in vitro experiments respectively to identify the potential of stigmasterol against MRSA. The data were analyzed statistically with one-way variance analysis (ANOVA), post-doc Tukey assay, correlation, and regression by SPSS Amos 24. In the in silico experiment, stigmasterol was strongly associated with penicillin-binding protein 2a (PBP2a) on the active side of LYS289. Thus, it had the potential to inhibit PBP2a. Disruption of cell walls, the release of cytoplasmic contents, and a decrease in the cellular volume of MRSA were caused by the inhibition of PBP2a. The well diffusion test results showed the inhibition zone in 50 - 100 μg/ml of stigmasterol against MRSA. The inhibition zone was in the range of 7.38 – 10.17 mm. The inhibition diameter indicated a significant difference with a p-value < 0.05. The results of statistical analyses indicate that stigmasterol is effective as an antimicrobial against MRSA. Stigmasterol has potential as a potential microbial agent for the therapy of MRSA.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140475440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kaspars Kampuss, P. Semjonovs, Sergejs Kolesovs, Kristīne Afoņina, Andris Bāliņš
The experiments were done at two farms in Latvia. Primocane raspberries were weekly sprayed with solutions of ethanol extractions of microalgae species of Spirulina sp., Dunaliella sp. and Chlorella sp. in different concentrations from stages of shoot growth to fruit ripening. During the season, dynamics of the shoot growth, beginning of flowering and fruit ripening were recorded. After the treatments, productive length of the shoots, number of flower trusses and leaves per shoot, number of productive shoots per plot, chlorophyll index, average fruit weight and sugar content in the fruits were measured. Certain positive effects of the treatments with one or another algae extraction were observed, increasing plant height and the proportion of the productive part of the shoot, number of leaves and flower trusses per shoot and the chlorophyll index. Moreover, no negative effects of any tested treatments were observed. However, the positive effects differed in different farms and years. That can be explained by uneven growth of the plants in the farms, weed and disease spread, changing weather conditions. Therefore, we can conclude that since the results are strongly promising, further experiments in controlled environments using preparations of varied extraction types, microalgae species and different treatment protocols should be carried out.
{"title":"Response of raspberries to treatments with Spirulina sp., Dunaliella sp. and Chlorella sp. extractions","authors":"Kaspars Kampuss, P. Semjonovs, Sergejs Kolesovs, Kristīne Afoņina, Andris Bāliņš","doi":"10.25303/1903rjbt051057","DOIUrl":"https://doi.org/10.25303/1903rjbt051057","url":null,"abstract":"The experiments were done at two farms in Latvia. Primocane raspberries were weekly sprayed with solutions of ethanol extractions of microalgae species of Spirulina sp., Dunaliella sp. and Chlorella sp. in different concentrations from stages of shoot growth to fruit ripening. During the season, dynamics of the shoot growth, beginning of flowering and fruit ripening were recorded. After the treatments, productive length of the shoots, number of flower trusses and leaves per shoot, number of productive shoots per plot, chlorophyll index, average fruit weight and sugar content in the fruits were measured. Certain positive effects of the treatments with one or another algae extraction were observed, increasing plant height and the proportion of the productive part of the shoot, number of leaves and flower trusses per shoot and the chlorophyll index. Moreover, no negative effects of any tested treatments were observed. However, the positive effects differed in different farms and years. That can be explained by uneven growth of the plants in the farms, weed and disease spread, changing weather conditions. Therefore, we can conclude that since the results are strongly promising, further experiments in controlled environments using preparations of varied extraction types, microalgae species and different treatment protocols should be carried out.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140477660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mathu Albert Jero, Sunil Jayalekshmi, Hari Neethu, S. S. Vishnu, Jayakumaran Nair Ananthakrishnan
Brassica oleracea L. var. capitata f. alba (Brassicaceae) is a nutrient rich leafy-headed cruciferous vegetable. The bioactivities of the various extracts such as aqueous, hexane, chloroform and methanol of this Indian cultivated vegetable have been investigated. The anticancer activity was tested in the specific cell lines (MCF-7, HCT116 and 3T3L1). The methanol and aqueous extracts showed the highest cytotoxicity found in the MCF7 cell line. The chloroform extract showed antibacterial activity against Gram-negative Escherichia coli, but not against Gram-positive Bacillus subtilis. The aqueous extract of the sample gives maximum antioxidant activity. The qualitative and quantitative analyses of these extracts were carried out and confirmed the presence of indole-3-carbinol in the methanol extract. The significant differentially expressed genes that involved in the treatment of indole-3-carbinol on MCF7 cell line have been identified from the GEO dataset analysis. This study strongly proves the correlation between the anticancer, antibacterial and antioxidant activities of the sample and the presence of the specific chemical compound indole-3-carbinol and its metabolic involvement in the human diet and digestive system.
{"title":"Evaluation of bioactivities and importance of indole-3-carbinol in the Brassica oleracea L. var. capitata f. alba extracts","authors":"Mathu Albert Jero, Sunil Jayalekshmi, Hari Neethu, S. S. Vishnu, Jayakumaran Nair Ananthakrishnan","doi":"10.25303/1903rjbt020030","DOIUrl":"https://doi.org/10.25303/1903rjbt020030","url":null,"abstract":"Brassica oleracea L. var. capitata f. alba (Brassicaceae) is a nutrient rich leafy-headed cruciferous vegetable. The bioactivities of the various extracts such as aqueous, hexane, chloroform and methanol of this Indian cultivated vegetable have been investigated. The anticancer activity was tested in the specific cell lines (MCF-7, HCT116 and 3T3L1). The methanol and aqueous extracts showed the highest cytotoxicity found in the MCF7 cell line. The chloroform extract showed antibacterial activity against Gram-negative Escherichia coli, but not against Gram-positive Bacillus subtilis. The aqueous extract of the sample gives maximum antioxidant activity. The qualitative and quantitative analyses of these extracts were carried out and confirmed the presence of indole-3-carbinol in the methanol extract. The significant differentially expressed genes that involved in the treatment of indole-3-carbinol on MCF7 cell line have been identified from the GEO dataset analysis. This study strongly proves the correlation between the anticancer, antibacterial and antioxidant activities of the sample and the presence of the specific chemical compound indole-3-carbinol and its metabolic involvement in the human diet and digestive system.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140475453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alpha amylases (EC 3.2.1.1.) hydrolyze polysaccharides like starch and glycogen to create oligosaccharides of different sizes by random cleavage of internal 1, 4-glucosidic linkages. These enzymes are produced by many different bacteria and fungi. In this investigation, α-amylase from Aspergillus flavus and Aspergillus oryzae was taken into consideration. Amino acid sequences and protein structure were retrieved from databases. Sequences of Aspergillus oryzae possess a high number of charged residues whereas Aspergillus flavus have a higher abundance of uncharged polar and hydrophobic amino acid residues. 3 common superfamilies were observed between both species. The alignment of sequences showed their similar conservative nature. Non-covalent intra-protein interactions like the salt bridge, aromatic-aromatic interactions, aromatic-sulfur interactions and cation-pi interactions helped to increase the stability of α-amylase of Aspergillus oryzae. The tunnels and cavities also help to increase the functionality and catalytic activity of α-amylase. This study will also be helpful for protein engineering.
{"title":"Molecular Characterization of Alpha-Amylase of Aspergillus flavus and Aspergillus oryzae: An In Silico Study","authors":"Amrita Banerjee, Debanjan Mitra, Biswajit Das, Mohapatra Pradeep Kumar Das, S. Samanta","doi":"10.25303/1903rjbt076085","DOIUrl":"https://doi.org/10.25303/1903rjbt076085","url":null,"abstract":"Alpha amylases (EC 3.2.1.1.) hydrolyze polysaccharides like starch and glycogen to create oligosaccharides of different sizes by random cleavage of internal 1, 4-glucosidic linkages. These enzymes are produced by many different bacteria and fungi. In this investigation, α-amylase from Aspergillus flavus and Aspergillus oryzae was taken into consideration. Amino acid sequences and protein structure were retrieved from databases. Sequences of Aspergillus oryzae possess a high number of charged residues whereas Aspergillus flavus have a higher abundance of uncharged polar and hydrophobic amino acid residues. 3 common superfamilies were observed between both species. The alignment of sequences showed their similar conservative nature. Non-covalent intra-protein interactions like the salt bridge, aromatic-aromatic interactions, aromatic-sulfur interactions and cation-pi interactions helped to increase the stability of α-amylase of Aspergillus oryzae. The tunnels and cavities also help to increase the functionality and catalytic activity of α-amylase. This study will also be helpful for protein engineering.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140476696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the rhizospheric soil of wild sugarcane from three different salt-affected soils of the eastern Indo-Gangetic Plains was sampled. Rhizospheric bacteria from S. spontaneum were isolated and they were characterized for growth-promoting traits with molecular identification. A total of 49 isolates were isolated from three S. spontaneum rhizosphere soils from different places. These isolates were shown to have differential tolerance to NaCl concentrations up to 2000 mM. Owing to their host habitat and poor nutrient availability, these bacteria were found to have excellent tolerance to high NaCl concentrations along with having multiple plant growth traits. Apart from salt tolerance, these isolates were screened for EPS production, phosphate solubilization, zinc solubilization, siderophore production and ammonia production and based on multiple plant growth-promoting traits, twenty isolates were taken for molecular identification and further in vivo screening. These twenty S. spontaneum rhizospheric microbes belonged to the classes Gammaproteobacteria, Firmicutes (Bacilli) and Actinomycetia, representing six genera and 15 species. In vivo growth promotion studies indicated that some of these isolates could improve germination and growth in rice seedlings. The results of this study signify the possibility of utilizing wild crop microbiomes to nurture cultivated crops under adverse growing conditions.
本研究对印度-甘肃平原东部三种不同盐渍土壤中野生甘蔗根瘤土壤进行了取样。从甘蔗根瘤中分离出根瘤细菌，并对其生长促进特性进行了分子鉴定。从不同地方的三种 S. spontaneum 根瘤土壤中分离出了 49 个分离菌。结果表明，这些分离物对高达 2000 mM 的 NaCl 浓度具有不同的耐受性。由于其寄主栖息地和养分供应不足，这些细菌被发现对高浓度 NaCl 具有极强的耐受性，同时还具有多种植物生长特性。除耐盐性外，还对这些分离菌进行了 EPS 生产、磷酸盐溶解、锌溶解、苷元生产和氨生产等方面的筛选，并根据多种促进植物生长的性状，提取了 20 个分离菌进行分子鉴定和进一步的体内筛选。这 20 个 S. spontaneum 根瘤微生物属于甘露聚糖菌类、真菌类（芽孢杆菌）和放线菌类，代表 6 个属 15 个种。体内生长促进研究表明，其中一些分离物可以提高水稻秧苗的发芽率和生长率。这项研究的结果表明，在不利的生长条件下，有可能利用野生作物微生物群来培育栽培作物。
{"title":"Exploring rhizobacteria of wild sugarcane (Saccharum spontaneum L.) and their plant growth promoting potential","authors":"Amrita Gupta, Arvind Nath Singh, Sujeet Pratap Singh, P. Sahu, Rajesh Kumar Tiwari","doi":"10.25303/1903rjbt930100","DOIUrl":"https://doi.org/10.25303/1903rjbt930100","url":null,"abstract":"In this study, the rhizospheric soil of wild sugarcane from three different salt-affected soils of the eastern Indo-Gangetic Plains was sampled. Rhizospheric bacteria from S. spontaneum were isolated and they were characterized for growth-promoting traits with molecular identification. A total of 49 isolates were isolated from three S. spontaneum rhizosphere soils from different places. These isolates were shown to have differential tolerance to NaCl concentrations up to 2000 mM. Owing to their host habitat and poor nutrient availability, these bacteria were found to have excellent tolerance to high NaCl concentrations along with having multiple plant growth traits. Apart from salt tolerance, these isolates were screened for EPS production, phosphate solubilization, zinc solubilization, siderophore production and ammonia production and based on multiple plant growth-promoting traits, twenty isolates were taken for molecular identification and further in vivo screening. These twenty S. spontaneum rhizospheric microbes belonged to the classes Gammaproteobacteria, Firmicutes (Bacilli) and Actinomycetia, representing six genera and 15 species. In vivo growth promotion studies indicated that some of these isolates could improve germination and growth in rice seedlings. The results of this study signify the possibility of utilizing wild crop microbiomes to nurture cultivated crops under adverse growing conditions.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140477592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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