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Evaluation of the Presence and Importance of PD-L1 Expression in Head-neck Squamosus Cell Carcinomas and Premalign Lesions PD-L1在头颈鳞状细胞癌和癌前病变中表达及其重要性的评估
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-27 DOI: 10.32592/ircmj.2023.25.6.2567
Background: Programmed Death-Ligand 1 (PD-L1) is a cell membrane protein found on the surface of cancer cells, immune system cells in the tumor microenvironment, and various healthy tissues. Moreover, it plays a key role in suppressing the immune system.Objectives: We aim to explain the presence of PD-L1 in Head-Neck Squamosus Cell Carcinomas (HNSCC) and premalign lesions by immunohistochemical method.Methods: Our retrospective study included 22 patients with HNSCC (15(68.1%) Oral cavity and 7(31.9%) Oropharynx), 20 patients with oral lichen planus, and 14 patients with normal oral cavity mucosa. In the evaluation of PD-L1 antibodies applied immunohistochemically in patients with HNSCC, the percentage of tumor cells showing membranous staining with PD-L1 antibodies was calculated.Results: The mean age of HNSCC patients participating in the study was 52.24 ± 11.7 years, the mean age of oral lichen planus patients was 34.10 ± 9.8 years, and the control patients' mean age was 31.42 ± 10.6 years. The rate of PD-L1 staining of tumor cells of HNSCC patients was significantly higher than the control group (P=0.001).Conclusion: The importance of PD-L1 expression in HNSCC and precancerous lesions of the oral cavity is remarkable, and the values may be related to the pathophysiology of these diseases.
背景:程序性死亡配体1(PD-L1)是一种存在于癌症细胞、肿瘤微环境中的免疫系统细胞和各种健康组织表面的细胞膜蛋白。此外,它在抑制免疫系统方面发挥着关键作用。目的:用免疫组织化学方法解释PD-L1在头颈鳞状细胞癌(HNSCC)和癌前病变中的存在。方法:我们的回顾性研究包括22例HNSCC患者(15例(68.1%)口腔和7例(31.9%)口咽)、20例口腔扁平苔藓患者和14例正常口腔粘膜患者。在应用免疫组织化学方法对HNSCC患者的PD-L1抗体进行评估时,计算了显示PD-L1抗体膜染色的肿瘤细胞的百分比。结果:参与研究的HNSCC患者平均年龄为52.24±11.7岁,口腔扁平苔藓患者平均年龄34.10±9.8岁,对照组患者平均年龄31.42±10.6岁。HNSCC患者肿瘤细胞PD-L1染色率明显高于对照组(P=0.001)。结论:PD-L1在HNSCC和口腔癌前病变中的表达具有重要意义,其价值可能与这些疾病的病理生理学有关。
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引用次数: 0
Efficacy of Laryngeal Mask Airway Impregnation with Diltiazem Gel on Hemodynamic Changes in Patients with Hypertension Undergoing Phacoemulsification Surgery: A Randomized Clinical Trial 喉罩气道滴注地尔硫卓凝胶对超声乳化术后高血压患者血液动力学变化的影响:随机临床试验
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-26 DOI: 10.32592/ircmj.2023.25.5.2359
Background: Airway control problems are among the most prevalent causes of anesthesia-related mortality and morbidity. Some devices provide patients with adequate oxygen supply and ventilation during surgery by creating a safe airway in anesthetized patients. One of these devices is the laryngeal mask airway (LMA). The compression and painful stimuli following the LMA cuff inflation can lead to hemodynamic changes. Diltiazem gel is used in the control and treatment of hypertension (HTN) and heart arrhythmia and is absorbed through the tracheal mucosa.Objectives: By assuming that diltiazem gel is superior to other drugs used to prevent arrhythmias and hemodynamic changes during surgery, this study aimed to evaluate the effect of LMA impregnation with diltiazem gel, compared to lubricant gel.Methods: This study was conducted as a double-blind, randomized clinical trial on 80 participants with HTN who were candidates for phacoemulsification (phaco) surgery in Imam Khomeini Hospital, Kermanshah, Iran. The participants were assigned to an intervention (LMA impregnated with diltiazem gel) and a control group (LMA impregnated with lubricant gel) through the block random method in the form of 40 blocks of 2 using a random-numbers table. Hemodynamic changes (systolic and diastolic blood pressure and heart rate) were measured before, immediately after, 5 min, and 15 min after intubation, during surgery every 15 min, upon entering the recovery unit, and 15 and 30 min after entering the recovery unit.Results: The mean systolic and diastolic blood pressure in the intervention group showed a significant decrease, compared to that in the control group. A significant difference was also observed in the mean heart rate difference between the two study groups, but only at the beginning of the study (P<0.05). Additionally, according to the results of repeated measures analysis of variance, the mean of the measured variables showed a significant difference at different measurement times in the intervention group (P<0.05).Conclusion: The findings supported the effectiveness of diltiazem gel in reducing blood pressure, especially in the final stages of surgery, decreasing the number of premature ventricular contractions, and controlling normal breathing. Therefore, specialists and surgeons can use diltiazem gel to control the hemodynamic status of patients.
背景:气道控制问题是麻醉相关死亡率和发病率的最常见原因之一。一些设备通过在麻醉患者中建立安全的气道,在手术期间为患者提供充足的氧气供应和通气。其中一种装置是喉罩气道(LMA)。LMA袖带充气后的压迫和疼痛刺激可能导致血液动力学变化。地尔硫卓凝胶用于控制和治疗高血压(HTN)和心律失常,并通过气管粘膜吸收。目的:通过假设地尔硫卓凝胶优于其他用于预防手术中心律失常和血液动力学变化的药物,本研究旨在评估与润滑剂凝胶相比,地尔硫卓凝胶浸渍LMA的效果。方法:本研究是一项双盲、随机的临床试验,对伊朗克尔曼沙赫伊玛目霍梅尼医院80名HTN患者进行超声乳化(phaco)手术。参与者被分配到干预组(用地尔硫卓凝胶浸渍的LMA)和对照组(用润滑剂凝胶浸渍的NMA),通过使用随机数表的40个区块的形式的区块随机方法。在插管前、插管后立即、插管后5分钟和插管后15分钟,在每15分钟一次的手术中,在进入恢复单元时,以及在进入康复单元后15分钟和30分钟测量血液动力学变化(收缩压和舒张压以及心率)。结果:与对照组相比,干预组的平均收缩压和舒张压显著下降。两个研究组的平均心率差异也有显著性差异,但仅在研究开始时(P<0.05)。此外,根据重复测量的方差分析结果,干预组在不同测量时间测量变量的平均值有显著差异(P<0.05)。结论:研究结果支持地尔硫卓凝胶在降低血压,尤其是在手术的最后阶段,减少室性早搏次数和控制正常呼吸方面的有效性。因此,专家和外科医生可以使用地尔硫卓凝胶来控制患者的血液动力学状态。
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引用次数: 0
Inhibition of Cancer Stem Cells Growth with Silibinin Encapsulated in Nanoparticles with Deregulation of miR-34a, miR-221, and miR-222 解除miR-34a、miR-221和miR-222调控的纳米颗粒包封水飞蓟宾抑制癌症干细胞生长
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-25 DOI: 10.32592/ircmj.2023.25.4.2476
Background: Colorectal Cancer (CRC) is the most common malignant gastrointestinal cancer. Cancer stem cells (CSCs) are the major cause of cancer recurrence and cancer drug resistance. Silibinin, as an herbal compound, has anticancer properties.Objectives: The present study aimed to evaluate the antiproliferative effects of silibinin on HT29 stem-like cells (spheroids).Methods: In this study, antiproliferative and apoptotic properties of Silibinin encapsulated in Polymersome Nanoparticles (SPNs) were evaluated by MTT assay, propidium iodide (PI) /AnnexinV assay, cell cycle analysis, and DAPI (4',6-diamidino-2-phenylindole) staining. The expression of some miRNAs and their potential targets was evaluated by real-time reverse transcription-polymerase chain reaction (qRT-PCR).Results: IC50 of SPNs was determined at 28.13±0.78µg/ml after 24 h. SPNs (28µg/ml) induced apoptosis by 32.36% in HT29 cells after 24 h. DAPI staining indicated a decrease in stained nuclei after SPNs induction. SPNs treatment increased the expression of miR-34a, as well as P53, BAX, CASP9, CASP3, and CASP8. The downregulation of miR-221 and miR-222 was observed in SPNs treated cells. Moreover, SPNs decrease the expression level of CD markers in HT29 spheroids (cancer stem cells) compared to untreated spheroids. Spheroids were completely destroyed after 72 h treatment with SPNs (28µg/ml). Conclusion: As evidenced by the obtained results, SPNs can be used as an effective anticancer agent in multi-layer (cancer stem cells) and mono-layer cancerous cells with the upregulation of tumor suppressive miRs and genes, as well as downregulation of oncomiRs and oncogenes.
背景:结直肠癌(Colorectal Cancer, CRC)是最常见的胃肠道恶性肿瘤。肿瘤干细胞(Cancer stem cells, CSCs)是肿瘤复发和耐药的主要原因。水飞蓟宾作为一种草药化合物,具有抗癌特性。目的:探讨水飞蓟宾素对HT29干细胞样细胞(球体)的抗增殖作用。方法:采用MTT法、碘化丙啶(PI) /AnnexinV法、细胞周期法和DAPI(4′,6-二氨基-2-苯基吲哚)染色,观察聚合体纳米颗粒(Polymersome Nanoparticles, SPNs)包封水飞蓟宾的抗增殖和凋亡特性。通过实时逆转录聚合酶链反应(qRT-PCR)评估部分mirna及其潜在靶点的表达。结果:24 h后SPNs的IC50为28.13±0.78µg/ml, SPNs(28µg/ml)诱导HT29细胞凋亡率为32.36%,DAPI染色显示SPNs诱导后染色细胞核减少。SPNs处理增加了miR-34a以及P53、BAX、CASP9、CASP3和CASP8的表达。在spn处理的细胞中观察到miR-221和miR-222的下调。此外,与未处理的球状体相比,SPNs降低了HT29球状体(癌症干细胞)中CD标志物的表达水平。SPNs(28µg/ml)处理72 h后,球体被完全破坏。结论:spn可作为多层(肿瘤干细胞)和单层癌细胞的有效抗癌药物,其抑瘤miRs和抑瘤基因上调,癌因子和癌基因下调。
{"title":"Inhibition of Cancer Stem Cells Growth with Silibinin Encapsulated in Nanoparticles with Deregulation of miR-34a, miR-221, and miR-222","authors":"","doi":"10.32592/ircmj.2023.25.4.2476","DOIUrl":"https://doi.org/10.32592/ircmj.2023.25.4.2476","url":null,"abstract":"Background: Colorectal Cancer (CRC) is the most common malignant gastrointestinal cancer. Cancer stem cells (CSCs) are the major cause of cancer recurrence and cancer drug resistance. Silibinin, as an herbal compound, has anticancer properties.\u0000\u0000Objectives: The present study aimed to evaluate the antiproliferative effects of silibinin on HT29 stem-like cells (spheroids).\u0000\u0000Methods: In this study, antiproliferative and apoptotic properties of Silibinin encapsulated in Polymersome Nanoparticles (SPNs) were evaluated by MTT assay, propidium iodide (PI) /AnnexinV assay, cell cycle analysis, and DAPI (4',6-diamidino-2-phenylindole) staining. The expression of some miRNAs and their potential targets was evaluated by real-time reverse transcription-polymerase chain reaction (qRT-PCR).\u0000\u0000Results: IC50 of SPNs was determined at 28.13±0.78µg/ml after 24 h. SPNs (28µg/ml) induced apoptosis by 32.36% in HT29 cells after 24 h. DAPI staining indicated a decrease in stained nuclei after SPNs induction. SPNs treatment increased the expression of miR-34a, as well as P53, BAX, CASP9, CASP3, and CASP8. The downregulation of miR-221 and miR-222 was observed in SPNs treated cells. Moreover, SPNs decrease the expression level of CD markers in HT29 spheroids (cancer stem cells) compared to untreated spheroids. Spheroids were completely destroyed after 72 h treatment with SPNs (28µg/ml). \u0000\u0000Conclusion: As evidenced by the obtained results, SPNs can be used as an effective anticancer agent in multi-layer (cancer stem cells) and mono-layer cancerous cells with the upregulation of tumor suppressive miRs and genes, as well as downregulation of oncomiRs and oncogenes.","PeriodicalId":48912,"journal":{"name":"Iranian Red Crescent Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42581635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the Effect of Inhalation and Intermittent Inhalation-Exhalation Incentive Spirometry on Dyspnea Severity and Atelectasis in Patients undergoing Coronary Artery Bypass Graft 吸入与间歇吸气-呼气刺激肺活量测定法对冠状动脉搭桥术患者呼吸困难程度和肺不张的影响比较
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-20 DOI: 10.32592/ircmj.2023.25.3.1918
Background: Dyspnea and atelectasis after Coronary Artery Bypass Graft (CABG) are common and need nursing attention. Inhalation and exhalation incentive spirometry is recommended for preventing alveolar collapse and atelectasis.Objectives: This study aimed to compare different methods of incentive spirometry on dyspnea severity and atelectasis of the patients undergoing CABG.Methods: This randomized clinical trial study was conducted on 66 patients undergoing CABG admitted to the Intensive Care Unit (ICU) of Cardiac Surgery Department in Hamadan, Iran, in 2020. The patients were selected through convenient sampling and were divided into two groups through block permutation: inhalation incentive spirometry and intermittent inhalation-exhalation spirometry. Both groups performed incentive spirometry according to the instructions they had received for four days. Then, they were examined for dyspnea and atelectasis using the Borg scale and chest x-ray, respectively.Results: During the intervention, dyspnea significantly decreased in the intermittent inhalation-exhalation group relative to the inhalation group (P<0.05). Most patients in both inhalation (63.6%) and intermittent inhalation-exhalation (65.6%) groups had atelectasis; however, no significant differences were observed between the two groups (P=0.867).Conclusion: Incentive spirometry via intermittent inhalation-exhalation method was more effective on dyspnea compared to the inhalation method. Therefore, this method is recommended to patients and nurses to reduce pulmonary complications after CABG
背景:冠状动脉搭桥术(CABG)术后呼吸困难和肺不张是常见的,需要注意护理。吸气和呼气刺激肺活量测定法被推荐用于预防肺泡塌陷和肺不张。目的:本研究旨在比较不同刺激肺活量测定法对冠脉搭桥患者呼吸困难程度和肺不张的影响。方法:本随机临床试验研究对2020年在伊朗哈马丹市心脏外科重症监护病房(ICU)住院的66例CABG患者进行了研究。采用方便抽样的方法将患者分为两组:吸入性肺活量测定法和间歇吸呼肺活量测定法。两组都根据他们收到的四天指示进行了激励性肺活量测定。然后,分别使用Borg评分和胸部x线检查呼吸困难和肺不张。结果:干预期间,间歇吸气-呼气组呼吸困难明显低于吸气组(P<0.05)。吸入组(63.6%)和间歇吸入呼出组(65.6%)大多数患者存在肺不张;两组间差异无统计学意义(P=0.867)。结论:间断性吸气-呼气法刺激肺活量测定治疗呼吸困难比吸入法更有效。因此,推荐给患者和护士,以减少CABG术后肺部并发症
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引用次数: 0
Efficacy of Hydroxychloroquine versus Clarithromycin in the Improvement of Dyspnea and Cough in Patients after the Treatment of Acute-Phase COVID-19 羟氯喹与克拉霉素对COVID-19急性期治疗后患者呼吸困难和咳嗽的疗效观察
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-20 DOI: 10.32592/ircmj.2023.25.4.2080
Background: Post-acute COVID-19 syndrome involves the persistence of the patient’s symptoms due to the residual inflammation of the acute phase.Objectives: In the current study, we aimed to evaluate medication intervention to accelerate the improvement of prolonged respiratory symptoms in this phase.Methods: Thirty-four patients, aged 20-50 years, in the recovery phase of COVID-19, were enrolled, who still suffered from respiratory problems even two weeks after being discharged from Rasool Akram Hospital, Tehran, Iran. They were divided into three groups based on the type of treatment for eliminating the remaining symptoms: hydroxychloroquine (HCQ, 200 mg twice daily for four weeks), clarithromycin (500 mg twice daily for four weeks), and control (receiving a placebo similar to the last two groups). At the beginning and end of the treatment, patients’ dyspnea and cough were assessed using Medical Research Council and visual analog scale (VAS), respectively, their laboratory tests were checked, and they took a 6-min walk test.Results: At the end of the treatment, the VAS of cough was 0.74 in the HCQ group, which was higher than that in the clarithromycin group. In addition, dyspnea decreased in the HCQ and clarithromycin groups by 64% and 40%, respectively, compared to the control group. Furthermore, there was a significant relationship between residual dyspnea at the end of the treatment and the severity of initial lung involvement in the acute phase.Conclusion: Based on these findings, it can be concluded that HCQ was more effective in reducing dyspnea, compared to clarithromycin, in the recovery phase, especially in patients with milder lung involvement in the acute phase. Additionally, clarithromycin was found to be more effective in improving coughs.
背景:COVID-19急性后综合征是指由于急性期炎症残留导致患者症状持续存在。目的:在本研究中,我们旨在评估药物干预以加速该阶段延长呼吸道症状的改善。方法:选取34例新冠肺炎恢复期患者,年龄20 ~ 50岁,出院2周后仍有呼吸问题。他们根据消除剩余症状的治疗类型分为三组:羟氯喹(HCQ,每天200毫克两次,持续四周)、克拉霉素(每天500毫克两次,持续四周)和对照组(接受与后两组相似的安慰剂)。在治疗开始和结束时,分别采用医学研究委员会(Medical Research Council)和视觉模拟量表(VAS)评估患者的呼吸困难和咳嗽,检查实验室检查,并进行6分钟步行测试。结果:治疗结束时,HCQ组咳嗽VAS评分为0.74,高于克拉霉素组。此外,与对照组相比,HCQ和克拉霉素组的呼吸困难分别减少了64%和40%。此外,治疗结束时的残余呼吸困难与急性期初始肺部受累的严重程度之间存在显著关系。结论:基于这些发现,与克拉霉素相比,在恢复期,特别是在急性期肺部受累较轻的患者中,HCQ可更有效地减轻呼吸困难。此外,克拉霉素被发现对改善咳嗽更有效。
{"title":"Efficacy of Hydroxychloroquine versus Clarithromycin in the Improvement of Dyspnea and Cough in Patients after the Treatment of Acute-Phase COVID-19","authors":"","doi":"10.32592/ircmj.2023.25.4.2080","DOIUrl":"https://doi.org/10.32592/ircmj.2023.25.4.2080","url":null,"abstract":"Background: Post-acute COVID-19 syndrome involves the persistence of the patient’s symptoms due to the residual inflammation of the acute phase.\u0000\u0000Objectives: In the current study, we aimed to evaluate medication intervention to accelerate the improvement of prolonged respiratory symptoms in this phase.\u0000\u0000Methods: Thirty-four patients, aged 20-50 years, in the recovery phase of COVID-19, were enrolled, who still suffered from respiratory problems even two weeks after being discharged from Rasool Akram Hospital, Tehran, Iran. They were divided into three groups based on the type of treatment for eliminating the remaining symptoms: hydroxychloroquine (HCQ, 200 mg twice daily for four weeks), clarithromycin (500 mg twice daily for four weeks), and control (receiving a placebo similar to the last two groups). At the beginning and end of the treatment, patients’ dyspnea and cough were assessed using Medical Research Council and visual analog scale (VAS), respectively, their laboratory tests were checked, and they took a 6-min walk test.\u0000\u0000Results: At the end of the treatment, the VAS of cough was 0.74 in the HCQ group, which was higher than that in the clarithromycin group. In addition, dyspnea decreased in the HCQ and clarithromycin groups by 64% and 40%, respectively, compared to the control group. Furthermore, there was a significant relationship between residual dyspnea at the end of the treatment and the severity of initial lung involvement in the acute phase.\u0000\u0000Conclusion: Based on these findings, it can be concluded that HCQ was more effective in reducing dyspnea, compared to clarithromycin, in the recovery phase, especially in patients with milder lung involvement in the acute phase. Additionally, clarithromycin was found to be more effective in improving coughs.","PeriodicalId":48912,"journal":{"name":"Iranian Red Crescent Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44159015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the Dexmedetomidine and Sufentanil Added to Spinal Anesthesia with Bupivacaine for Hemodynamic Stability and Postoperative Analgesia in Elective Cesarean Section Surgery: A Single-Blinded Randomized Clinical Trial 右美托咪定和舒芬太尼加布比卡因脊髓麻醉对剖宫产手术血流动力学稳定性和术后镇痛的比较:一项单盲随机临床试验
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-20 DOI: 10.32592/ircmj.2023.25.3.2361
Background: Hemodynamic instability, including hypotension and bradycardia, can occur due to spinal anesthesia by bupivacaine. However, it is possible to reduce the chance of hemodynamic changes by the reduction of the dose of bupivacaine and/or the addition of adjunctive agents.Objective: This study aimed to compare the effects of the addition of intrathecal dexmedetomidine and sufentanil to spinal anesthesia with bupivacaine in elective cesarean section (CS).Methods: This prospective single-blinded randomized clinical trial with parallel groups was performed on 60 pregnant women who were candidates for elective CS in Imam Reza Hospital in Kermanshah, Iran. In addition to receiving 10 mg of hyperbaric bupivacaine 0.5%, they were randomly divided into two groups to receive intrathecal sufentanil 5 μg (30 cases) or dexmedetomidine 5 μg (30 cases). Changes in blood pressure, heart rate, and occurrence of side effects (e.g., nausea, vomiting, headache, and shivering) were recorded within 1 h after the injections. Moreover, the postoperative analgesia rate and duration (using a visual analog scale [VAS]) were recorded within the first 24 h after the completion of the CS. Headache severity (using a VAS) was also measured during the first week after CS.Results: Patients declared their satisfaction with analgesia after surgery. No significant difference was found between the two groups in terms of heart rate and systolic and diastolic blood pressure changes during the first 60 min. Similarly, no significant difference was observed between the two groups in terms of the severity of incision pain in the first 24 h after CS surgery. On days 3, 4, and 5, none of the patients in the sufentanil and bupivacaine group had headaches (VAS=0), but patients in the dexmedetomidine and bupivacaine group had some degree of headache (P=0.040).Conclusions: Based on the results, intrathecal administration of bupivacaine with either sufentanil or dexmedetomidine in CS did not have significantly different effects, except for slightly more severe headaches in the dexmedetomidine group. Therefore, no superiority of one drug over the other was observed for intrathecal administration with bupivacaine in CS.
背景:布比卡因腰麻可引起血液动力学不稳定,包括低血压和心动过缓。然而,可以通过减少布比卡因的剂量和/或添加辅助剂来减少血液动力学变化的机会。目的:本研究旨在比较鞘内注射右美托咪定和舒芬太尼在布比卡因脊麻选择性剖宫产(CS)中的效果。除给予10mg 0.5%高压布比卡因外,随机分为两组,鞘内给予舒芬太尼5μg(30例)或右美托咪定5μg。在注射后1小时内记录血压、心率和副作用(如恶心、呕吐、头痛和颤抖)的变化。此外,在CS完成后的前24小时内记录术后镇痛率和持续时间(使用视觉模拟评分[VAS])。在CS后的第一周,还测量了头痛的严重程度(使用VAS)。结果:患者在术后对镇痛表示满意。两组在前60分钟的心率、收缩压和舒张压变化方面没有发现显著差异。同样,在CS手术后的前24小时,两组在切口疼痛的严重程度方面也没有观察到显著差异。在第3天、第4天和第5天,舒芬太尼和布比卡因组的患者均无头痛(VAS=0),但右美托咪定和布比卡因组的患者有一定程度的头痛(P=0.040),除了右美托咪定组稍微更严重的头痛。因此,在CS鞘内给药布比卡因时,未观察到一种药物优于另一种药物。
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引用次数: 0
Effects of the Covid-19 Pandemic on Patients with Fournier's Gangrene 新冠肺炎大流行对Fournier Gangrene患者的影响
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-18 DOI: 10.32592/ircmj.2023.25.6.2334
Background: The first case of COVID-19 infection in Turkey was reported on March 11th, 2020, and declared a pandemic by the World Health Organization (WHO) in March 2020, introducing new regulations to national health systems. Some patients with non-COVID-19 presentations may have been adversely affected by this pandemic.Objectives: The present study aimed to investigate the effect of COVID-19 on patients with Fournier's gangrene during the pandemic and the impact of the COVID-19 pandemic on the clinical management and patient outcomes for Fournier's gangrene.Methods: This retrospective cross-sectional study was conducted between March 2018 and March 2022 at the General Surgery Department of Trakya University. Patients were stratified into pre-pandemic and pandemic groups based on the date of March 11th, 2020, when the first Covid-19 case was reported in Turkey. Data collection and retrospective analysis were completed for all patients who were operated on for Fournier's gangrene originating from the perianal region. Demographic characteristics, predisposing factors, as well as laboratory and clinical results of the patients treated during the pandemic, were compared with the patients treated before the pandemic.Results: A total of 43 patients were included in the study (pre-pandemic: 24, pandemic: 19). There was a statistically significant difference between the pre-pandemic and pandemic groups in terms of the median length of hospital stay (7 vs. 16 days, p<0.001) and the median number of debridement (4 vs. 2, p=0.002).Conclusion: In the presence of life-threatening surgical pathologies, such as Fournier's gangrene, the number of admissions did not decrease despite the pandemic. Precautions taken to reduce the risk of transmission in pandemic conditions and more aggressive surgical applications can reduce the number of debridement procedures and shorten the length of hospital stay. Subsequently, this is associated with similar treatment outcomes, lower morbidity, and reduced treatment costs.
背景:土耳其于2020年3月11日报告了首例COVID-19感染病例,并于2020年3月被世界卫生组织(世卫组织)宣布为大流行,并向国家卫生系统引入了新的法规。一些没有covid -19症状的患者可能受到这次大流行的不利影响。目的:本研究旨在探讨COVID-19大流行期间对富尼耶坏疽患者的影响,以及COVID-19大流行对富尼耶坏疽临床管理和患者预后的影响。方法:本回顾性横断面研究于2018年3月至2022年3月在Trakya大学普通外科进行。根据2020年3月11日土耳其报告首例Covid-19病例的日期,将患者分为大流行前和大流行组。对所有因发源于肛周的富尼耶坏疽而行手术的患者进行资料收集和回顾性分析。将大流行期间接受治疗的患者的人口学特征、易感因素以及实验室和临床结果与大流行前接受治疗的患者进行比较。结果:共有43例患者被纳入研究(大流行前:24例,大流行:19例)。大流行前组和大流行组在住院时间的中位数(7天对16天,p<0.001)和清创次数的中位数(4天对2天,p=0.002)方面存在统计学上的显著差异。结论:在存在危及生命的外科病理,如富尼耶坏疽,入院人数没有减少,尽管大流行。在大流行条件下为减少传播风险而采取的预防措施和更积极的手术应用可减少清创手术的次数并缩短住院时间。随后,这与相似的治疗结果、较低的发病率和较低的治疗费用有关。
{"title":"Effects of the Covid-19 Pandemic on Patients with Fournier's Gangrene","authors":"","doi":"10.32592/ircmj.2023.25.6.2334","DOIUrl":"https://doi.org/10.32592/ircmj.2023.25.6.2334","url":null,"abstract":"Background: The first case of COVID-19 infection in Turkey was reported on March 11th, 2020, and declared a pandemic by the World Health Organization (WHO) in March 2020, introducing new regulations to national health systems. Some patients with non-COVID-19 presentations may have been adversely affected by this pandemic.\u0000\u0000Objectives: The present study aimed to investigate the effect of COVID-19 on patients with Fournier's gangrene during the pandemic and the impact of the COVID-19 pandemic on the clinical management and patient outcomes for Fournier's gangrene.\u0000\u0000Methods: This retrospective cross-sectional study was conducted between March 2018 and March 2022 at the General Surgery Department of Trakya University. Patients were stratified into pre-pandemic and pandemic groups based on the date of March 11th, 2020, when the first Covid-19 case was reported in Turkey. Data collection and retrospective analysis were completed for all patients who were operated on for Fournier's gangrene originating from the perianal region. Demographic characteristics, predisposing factors, as well as laboratory and clinical results of the patients treated during the pandemic, were compared with the patients treated before the pandemic.\u0000\u0000Results: A total of 43 patients were included in the study (pre-pandemic: 24, pandemic: 19). There was a statistically significant difference between the pre-pandemic and pandemic groups in terms of the median length of hospital stay (7 vs. 16 days, p<0.001) and the median number of debridement (4 vs. 2, p=0.002).\u0000\u0000Conclusion: In the presence of life-threatening surgical pathologies, such as Fournier's gangrene, the number of admissions did not decrease despite the pandemic. Precautions taken to reduce the risk of transmission in pandemic conditions and more aggressive surgical applications can reduce the number of debridement procedures and shorten the length of hospital stay. Subsequently, this is associated with similar treatment outcomes, lower morbidity, and reduced treatment costs.","PeriodicalId":48912,"journal":{"name":"Iranian Red Crescent Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48221866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Nurses’ Challenges during Coronavirus Outbreak: A Qualitative Study 冠状病毒爆发期间护士面临的挑战评估:一项定性研究
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-17 DOI: 10.32592/ircmj.2023.25.6.2687
Background: During the crisis of communicable diseases, nurses play an important role in controlling the disease and treating patients; therefore, the obstacles and challenges facing these medical personnel should be reduced.Objectives: The main goal of the present study was to evaluate the challenges faced by nurses during the Coronavirus Disease 2019 (COVID-19) pandemic.Methods: This qualitative study was conducted using the content analysis approach in 2021-22 in the city ABC. The participants in this study were 30 people who were selected from 10 different hospitals and included nursing managers (n=10), supervisors (n=10), and nurses (n=10). Sampling was done purposefully and continued until the information saturation point. Semi-structured in-depth interview was used individually based on the interview guideline to collect data.Results: The results showed that the challenges faced by nurses during the COVID-19 pandemic included 452 codes and 12 subcategories, which after the final classification of the data, 4 main challenge categories were obtained, which included human challenges (33 subcategories), financial challenges (15 subcategories), communication challenges (8 subcategories), and organizational challenges (27 subcategories).Conclusion: Based on the results of the present study, it can be concluded that the challenges faced by nurses during the COVID-19 pandemic included human, financial, communication, and organizational obstacles. Recognizing the challenges can help formulate road maps and strategies for improving disaster preparedness and management in hospitals.
背景:在传染病危机中,护士在控制疾病和治疗患者方面发挥着重要作用;因此,应减少这些医务人员面临的障碍和挑战。目的:本研究的主要目的是评估2019冠状病毒病(COVID-19)大流行期间护士面临的挑战。方法:采用内容分析法对2021- 2022年城市ABC进行定性研究。本研究的参与者为30人,分别来自10家不同的医院,包括护理经理(n=10)、主管(n=10)和护士(n=10)。采样是有目的的,一直持续到信息饱和点。采用半结构化深度访谈法,根据访谈指南进行数据收集。结果:结果显示,COVID-19大流行期间护士面临的挑战包括452个代码和12个子类,经数据最终分类后,获得4个主要挑战类,其中包括人力挑战(33个子类)、财务挑战(15个子类)、沟通挑战(8个子类)和组织挑战(27个子类)。结论:根据本研究结果,可以得出护士在COVID-19大流行期间面临的挑战包括人力,资金,沟通和组织障碍。认识到这些挑战有助于制定路线图和战略,以改进医院的备灾和管理。
{"title":"Evaluation of Nurses’ Challenges during Coronavirus Outbreak: A Qualitative Study","authors":"","doi":"10.32592/ircmj.2023.25.6.2687","DOIUrl":"https://doi.org/10.32592/ircmj.2023.25.6.2687","url":null,"abstract":"Background: During the crisis of communicable diseases, nurses play an important role in controlling the disease and treating patients; therefore, the obstacles and challenges facing these medical personnel should be reduced.\u0000\u0000Objectives: The main goal of the present study was to evaluate the challenges faced by nurses during the Coronavirus Disease 2019 (COVID-19) pandemic.\u0000\u0000Methods: This qualitative study was conducted using the content analysis approach in 2021-22 in the city ABC. The participants in this study were 30 people who were selected from 10 different hospitals and included nursing managers (n=10), supervisors (n=10), and nurses (n=10). Sampling was done purposefully and continued until the information saturation point. Semi-structured in-depth interview was used individually based on the interview guideline to collect data.\u0000\u0000Results: The results showed that the challenges faced by nurses during the COVID-19 pandemic included 452 codes and 12 subcategories, which after the final classification of the data, 4 main challenge categories were obtained, which included human challenges (33 subcategories), financial challenges (15 subcategories), communication challenges (8 subcategories), and organizational challenges (27 subcategories).\u0000\u0000Conclusion: Based on the results of the present study, it can be concluded that the challenges faced by nurses during the COVID-19 pandemic included human, financial, communication, and organizational obstacles. Recognizing the challenges can help formulate road maps and strategies for improving disaster preparedness and management in hospitals.","PeriodicalId":48912,"journal":{"name":"Iranian Red Crescent Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46246139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of coagulopathy frequency and risk factors in trauma patients in intensive care unit 重症监护病房创伤患者凝血功能障碍发生率及危险因素评价
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-15 DOI: 10.32592/ircmj.2023.25.3.2533
Background: Post traumatic coagulopathy is a frequently encountered problem. However, risk factors related to coagulopathy are not exactly known.Objectives: In this study it is targeted to determine the frequency of coagulopathy related to trauma, risk factors and its affect to prognosis on patients admitted to Intensive Care Unit(ICU) after trauma retrospectively.Methods: 184 patients admitted to mixed type adult ICU, which is having 20 beds, in the years between 2011 and 2017 due to trauma have been studied. Patients have been separated into two groups as patients having coagulopathy and not having coagulopathy by examining their laboratory results. Patients having; thrombocyte count <100 000/μL on the first and third days, Prothrombin Time(PT)>16s, activated Partial Thromboplastine Time(aPTT)>40s, International Normalized Ratio(INR)>1.6 are being accepted as having coagulopathy. Medical records of patients have been recorded retrospectively.Results: The average age of 184 patients that are included in the study is 41.77; 19% of them are Female and 81% of them are Male. Coagulopathy detected on 78 patients (42.4%) (on the first day 32.6%, on the third day 9.8%). It has been detected that, patients developed coagulopathy were having more accompanying diseases(p<0.05), lower Glasgow Coma Score (GCS)(p<0.05), lower albumin values(p<0.05) and higher 28-day mortality rates(p<0.001) compared to patients that have not developed coagulopathy. Revised Trauma Score(RTS) of two groups are not significant(p>0.05). Having accompanying diseases, having low GCS and albumin values are being determined as independent risk factors in coagulopathy development.Conclusion: As a conclusion; since it is not possible to change GCS and risk factors belong to critical trauma patients, we think that it is possible to decrease the frequency of coagulopathy development as well as mortality rate by preventing risk factors such as hypoalbuminemia by means of suitable approaches.
背景:创伤后凝血功能障碍是一个常见的问题。然而,与凝血功能障碍相关的危险因素尚不清楚。目的:回顾性分析创伤后入住重症监护病房(ICU)的患者发生创伤相关凝血功能障碍的频率、危险因素及其对预后的影响。方法:对2011 ~ 2017年收治的20张床位的混合型成人ICU收治的184例外伤患者进行分析。通过检查实验室结果,将患者分为两组:有凝血功能障碍的患者和没有凝血功能障碍的患者。病人有;凝血细胞计数16s,活化的部分凝血质体时间(aPTT) bbb400s,国际标准化比率(INR)>1.6被认为是凝血病。对患者病历进行回顾性记录。结果:184例纳入研究的患者平均年龄为41.77岁;其中19%是女性,81%是男性。78例患者(42.4%)出现凝血功能障碍(第1天32.6%,第3天9.8%)。发现发生凝血功能障碍的患者伴发疾病较多(p0.05)。伴随疾病、低GCS和白蛋白值被确定为凝血病发展的独立危险因素。结论:作为结论;由于GCS无法改变,且危险因素属于危重性创伤患者,我们认为通过适当的途径预防低白蛋白血症等危险因素,有可能降低凝血功能障碍的发生频率和死亡率。
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引用次数: 0
Construction of a Quality Evaluation system for Emergency Pre-examination and Triage under the Normalization of the COVID-19 Epidemic based on the SERVQUAL Model 基于SERVQUAL模型的疫情常态化下应急预检分诊质量评价体系构建
IF 0.4 4区 医学 Q Medicine Pub Date : 2023-03-15 DOI: 10.32592/ircmj.2023.25.3.2483
HongQuan Fan, Zhe Chu, Wei Wang, Huimin Li
Background: The effect of emergency epidemic prevention and control and the quality of emergency pre-testing and triage under the normalization of COVID-19 epidemics was evaluated by quality evaluation system based on the SERVQUAL model.Objectives: This study aimed to develop a quality evaluation system for emergency pre-test and triage under a normal COVID-19 epidemic, based on the SERVQUAL model.Methods: Based on the SERVQUAL model, a quality evaluation system of emergency pre-examination and triage under the normalization of the COVID-19 epidemic was developed. Two rounds of expert consultation were conducted by the Delphi method, involving 15 experts.Results: The recovery rates for the two rounds of expert consultation were 100 %, the expert authority coefficient was 0.954, and the Kendall coordination coefficients were 0.273 and 0.182. The coefficients of variation of the three-level indicators of the two rounds of expert consultation were 0.003-0.275 and 0.052-0.125, respectively. An evaluation system including 5 first-level indicators, 14 second-level indicators, and 42 third-level indicators of 5 dimensions (tangibility, reliability, responsiveness, assurance, and empathy) of the SERVQUAL model was established.Conclusion: Based on the SERVQUAL model, a quality evaluation system for emergency pre-examination and triage under the normalization of the COVID-19 epidemic situation was completed. It reflected the medical services, triage, and epidemic prevention and control required to provide the requisite quality of emergency pre-examination and triage under the normalization of the COVID-19 epidemic situation. It provided a reference for the quality supervision of emergency pre-examination and triage under the normalization of the COVID-19 epidemic situation.
背景:采用基于SERVQUAL模型的质量评价体系,对新冠肺炎疫情常态化背景下的应急疫情防控效果和应急预检分诊质量进行评价。目的:建立基于SERVQUAL模型的新型冠状病毒肺炎(COVID-19)正常疫情下应急预检测与分诊质量评价体系。方法:基于SERVQUAL模型,构建新冠肺炎疫情常态化下应急预检分诊质量评价体系。采用德尔菲法进行了两轮专家咨询,共有15名专家参与。结果:两轮专家咨询回收率均为100%,专家权威系数为0.954,肯德尔协调系数分别为0.273和0.182。两轮专家咨询的三级指标变异系数分别为0.003 ~ 0.275和0.052 ~ 0.125。建立SERVQUAL模型的5个一级指标、14个二级指标和42个三级指标(有形性、可靠性、响应性、保证性和共情性)的评价体系。结论:基于SERVQUAL模型,构建了新冠肺炎疫情常态化下应急预检分诊质量评价体系。反映了疫情常态化下,提供应急预检和分诊所需质量的医疗服务、分诊和疫情防控要求。为新冠肺炎疫情常态化下的应急预检分诊质量监督提供参考。
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引用次数: 0
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Iranian Red Crescent Medical Journal
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