The objective of this study was to identify the factors that best predict variations in tension, irritability, and fatigue (TIF) among university professors in Ecuador. Using a quantitative approach with a non-experimental, cross-sectional design, data were collected from a probabilistic sample of 364 participants. Psychometric measures were adapted and linguistically validated to assess TIF, and participants completed the Perceived Stress Questionnaire, alongside a sociodemographic questionnaire. Written informed consent was obtained, and participation was entirely voluntary. The results indicated that TIF significantly contribute to perceived stress levels among professors. Specific sociodemographic predictors were identified as statistically significant, providing insight into the multifaceted nature of work-related stress in academic settings and its potential implications for health and job satisfaction. The findings underscore the importance of targeted strategies to reduce stress-related outcomes, addressing factors unique to the academic environment in Ecuador. Additionally, while sociodemographic aspects were associated with variations in stress levels, other stress types also triggered TIF among university professors.