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Molecular characterization of Cassava (Manihot esculenta Crantz) with yellow-orange roots for beta-carotene improvement 黄橙色根木薯(Manihot esculenta Crantz)改善β -胡萝卜素的分子特性
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2008-03-30 DOI: 10.12702/1984-7033.V08N01A04
C. Ferreira, E. Alves, K. N. Pestana, D. Junghans, A. K. Kobayashi, V. J. Santos, R. P. Silva, Paulino Silva, E. Soares, W. Fukuda
Casssava (Manihot esculenta Crantz) is one of the main food and income sources of about 500 million people in the tropics. The crop is mainly cultivated by small farmers in tropical Africa, Asia and Latin America. Embrapa Mandioca e Fruticultura Tropical, based in Cruz das Almas, Bahia, maintains one of the largest cassava genebanks of Latin America. Among the accessions it contains, those with yellow-orange root color are particularly interesting. The objective of this study was to characterize 30 cassava accessions with yellow-orange root color by RAPD markers. The genetic distances of the 47 analyzed primers varied from 9.0 to 31.7 %, demonstrating the existing genetic variability to be exploited for the development of cassava varieties with higher beta-carotene contents. Molecular characterization of Cassava (Manihot esculenta Crantz) with yellow-orange roots for beta-carotene improvement Claudia Fortes Ferreira1*, Elaine Alves2, Katia Nogueira Pestana3, Davi Theodoro Junghans1, Adilson Kenji Kobayashi4, Vania de Jesus Santos3, Raimundo Pereira Silva1, Paulo Henrique Silva3, Ediclan Soares3, and Wania Fukuda1 1 Embrapa Mandioca e Fruticultura Tropical, s/n, C.P. 007, 44.380-000, Cruz das Almas, BA, Brasil, *E-mail: claudiaf@cnpmf.embrapa.br 2 Universidade Federal da Bahia (UFB), Rua Basilio da Gama, s/n, Canela, 40.110-907, Salvador, BA, Brasil 3 Universidade Federal do Reconcavo Baiano (UFRB), Campus Universitario da UFRB, 44.380-000, Cruz das Almas, BA, Brasil 4 Embrapa Meio-Norte, Av. Duque de Caxias, 5650, Buenos Aires, 64.006-220, Teresina, PI, Brasil INTRODUCTION Manihot esculenta Crantz is a species native to tropical America (Olsen and Schaal 2001), initially cultivated by native Latin Americans and later introduced into the African and Asian continents. The worldwide cassava production, on an area of 17.870.626 hectares is approximately 195.574.112 tons (FAO 2004). It is considered one of the most important sources of calories and is an inexpensive staple food in Latin America (Montero 2003), mainly in the northeastern region of Brazil (Mendes et al. 2006). The genetic diversity of this species is wide (Nassar 2006), concentrated mainly in Latin America and the Caribbean. Approximately 8500 cassava accessions are maintained worldwide in different collections, of which 7500 in South America (Costa and Morales 1994). Received 24 January 2007 Accepted 07 November 2007 In Brazil, 4132 accessions have been collected and are maintained in genebanks across the country (Fukuda 2000). Carotenes (-carotene, b-carotene, lycopene) represent the most multifaceted group of pigments in nature, with colors varying from yellow to red, found in photosynthetic and non-photosynthetic tissues, such as roots, seeds and fruits. Once ingested, b-carotene is transformed, in the liver, into Vitamin A. Vitamin A is a micro-nutrient with functions related to vision, cell differentiation, growth development, reproduction and the immune system. Vitamin A deficien
木薯(Manihot esculenta Crantz)是热带地区约5亿人的主要食物和收入来源之一。这种作物主要由热带非洲、亚洲和拉丁美洲的小农种植。位于巴伊亚州Cruz das Almas的Embrapa Mandioca e fruticulture Tropical拥有拉丁美洲最大的木薯基因库之一。其中根色为黄橙色的品种尤为有趣。本研究利用RAPD标记对30份根色为黄橙色的木薯材料进行了鉴定。47个引物的遗传距离在9.0% ~ 31.7%之间变化,表明存在遗传变异,可用于开发高β -胡萝卜素含量木薯品种。Claudia Fortes Ferreira1*, Elaine Alves2, Katia Nogueira pestan3, Davi Theodoro Junghans1, Adilson Kenji kobayash4, Vania de Jesus Santos3, Raimundo Pereira Silva1, Paulo Henrique Silva3, Ediclan soare3, Wania fuud1 . Embrapa Mandioca e Fruticultura Tropical, s/n, C.P. 007, 4438 -000, Cruz das Almas, BA, Brasil, * e - e:claudiaf@cnpmf.embrapa.br 2巴伊亚联邦大学(UFB),巴西巴西伽马大道,萨尔瓦多,40.110-907 3巴伊亚联邦大学(UFRB), UFRB大学,4438 -000,Cruz das Almas, BA,巴西4 Embrapa Meio-Norte, Av. Duque de Caxias, 5650,布宜诺斯艾利斯,64.006-220,Teresina, PI,巴西介绍manhot esculenta Crantz是热带美洲的原生物种(Olsen and Schaal 2001)。最初由拉丁美洲土著种植,后来被引入非洲和亚洲大陆。全球木薯产量约为195.574.112吨,面积为17.870.626公顷(粮农组织2004年)。它被认为是最重要的热量来源之一,在拉丁美洲是一种廉价的主食(Montero 2003),主要在巴西东北部地区(Mendes et al. 2006)。该物种的遗传多样性很广(Nassar 2006),主要集中在拉丁美洲和加勒比地区。全世界大约有8500种不同种类的木薯,其中7500种在南美洲(Costa和Morales, 1994年)。在巴西,已经收集了4132份材料,并保存在全国各地的基因库中(Fukuda 2000)。胡萝卜素(-胡萝卜素、b-胡萝卜素、番茄红素)是自然界中最具多样性的色素,其颜色从黄色到红色不等,存在于光合作用和非光合作用组织中,如根、种子和果实。一旦摄入,b-胡萝卜素在肝脏中转化为维生素A。维生素A是一种微量营养素,具有与视力、细胞分化、生长发育、繁殖和免疫系统有关的功能。维生素A缺乏症(VAD)可引起严重疾病,例如眼综合征、干眼症,并发展为不可逆的失明(Underwood等人,1999年)。虽然缺乏维生素A是可以预防的,但在许多发展中国家,干眼症仍然是一个公共卫生问题(Welch和Graham 2002)。
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引用次数: 42
Effects of endogamy on microsporogenesis in popcorn (Zea mays L.) 内婚制对玉米小孢子发生的影响
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2007-09-30 DOI: 10.12702/1984-7033.V07N03A12
M.F.P.T.B. Silva, E. Lopes, M. S. Pagliarini, C. A. Scapiml
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引用次数: 2
Genetic variation among and within sweet corn populations detected by RAPD and SSR markers 利用RAPD和SSR标记检测甜玉米群体间和群体内的遗传变异
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2005-12-31 DOI: 10.12702/1984-7033.V05N04A07
A. Sebbenn, A. C. S. Zanatto, M. L. M. Freitais, A. S. Sato, L. C. Ettori
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引用次数: 21
Estatística genômica aplicada a populações derivadas de cruzamentos controlados 应用于控制杂交群体的基因组统计
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2005-06-30 DOI: 10.12702/1984-7033.V05N02A19
I. Schuster, C. D. Cruz
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引用次数: 54
Selection of Pinus caribaea var. bahamensis progenies based on the predicted genetic value 基于预测遗传值的加勒比松子代选择
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2004-12-31 DOI: 10.12702/1984-7033.V04N04A04
R. F. Missio, L. Antônio, Santos Henrique Brant Dias, Luiz Teixeira de Moraes
Pinus caribaea var. bahamensis progenies were selected by mixed models using the estimate of the genetic parameters by REML for the prediction of additive genetic values by BLUP. The experimental design was a 11 x 11 partially balanced square lattice containing 119 progenies and two commercial controls. After 13 years, the diameter at breast height (DBH), height and volume were evaluated. The progenies presented good mean performance (18.83 m, 20.80 cm and 0.462 m 3 tree -1 ), and heritability estimates of 0.12, 0.20 and 0.21 for height, DBH and volume, respectively. The trait with greatest variability was the volume w ith a genetic variation coefficient of 22.60% while the genetic variation for height was lower (3.73%). A genetic gain of 14.5% was obtained for DBH with a selection of 250 trees from 56 progenies, with a mean of 4.46 trees selected per progeny and an effecti ve population size of 72.
利用REML估计的遗传参数,采用混合模型选择巴哈密松子代,用BLUP预测加性遗传值。实验设计是一个11 x 11的部分平衡方形格子,包含119个子代和两个商业对照。13年后,测定乳房高度直径(DBH)、高度和体积。后代平均表现良好(18.83 m, 20.80 cm和0.462 m 3 tree -1),高,胸径和体积的遗传力分别为0.12,0.20和0.21。变异系数最大的性状是体积w,遗传变异系数为22.60%,变异系数较小的性状是高度w,遗传变异系数为3.73%。从56个子代中选择250棵树,平均每个子代选择4.46棵树,有效群体大小为72棵,获得了14.5%的遗传增益。
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引用次数: 13
Genetic improvement of cacao 可可的遗传改良
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2004-12-31 DOI: 10.12702/1984-7033.V04N04A21
P. Griffee, É. Guimarães
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引用次数: 27
‘Conilon Vitória - Incaper 8142’: improved Coffea canephora var. kouillou clone cultivar for the state of Espírito Santo ' Conilon Vitória - Incaper 8142 ':为Espírito Santo州改良的canephora var. kouillou无性栽培品种
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2004-12-31 DOI: 10.12702/1984-7033.V04N04A20
A. Fonseca, M. A. Ferrão, R. G. Ferrão, A. C. V. Filho, Paulo, Sérgio Volpi, Francisco Zucateli
Conilon Vitória Incaper 8142 is a Coffea canephora var. kouillou clone type cultivar developed by Incaper, composed by clustering 13 superior clones. It presents a mean productivity of 70.4 bags of 60 kg ha, broad environmental adaptation and production stability, drought tolerance, uniformity of fruit maturation, and moderate rust resistance.
Conilon Vitória Incaper 8142是Incaper由13个优良无性系聚类而成的咖啡品种。它的平均产量为70.4袋,60公斤公顷,具有广泛的环境适应性和生产稳定性,耐旱性,果实成熟均匀性和中等的抗锈性。
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引用次数: 45
Yield stability of soybean lines using additive main effects and multiplicative interaction analysis - AMMI 用加性主效应和乘性互作分析大豆品系产量稳定性
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2004-12-31 DOI: 10.12702/1984-7033.V04N04A03
M. M. Rocha, N. Vello, Â. C. Almeida, Lopes, M. C. C. Maia
nes (G) using additive main effects and multiplicative interaction analysis (AMMI). Effects of the G, E, and GE interaction were found to be significant and accounted for 51, 12, and 36% of the variation, respectively. The first and only significant interaction princi pal component axis (IPCA1) accounted for 26% of the sum of squares due to original GE interaction. This concentrated the largest proportion of the pattern of GE interaction. Environments associated with Anhembi and Esalq proved more favorable, while Areao contributed negatively to the grain yield. However, Anhembi and Areao were more predictable for the crop years. USP 93-5082 and USP 93-5243 lines combined high adaptability and stability.
使用加性主效应和乘法相互作用分析(AMMI)。G、E和GE相互作用的影响是显著的,分别占变异的51%、12%和36%。第一个也是唯一一个显著的相互作用主分量轴(IPCA1)占原始GE相互作用平方和的26%。这集中了最大比例的GE相互作用模式。与Anhembi和Esalq相关的环境对粮食产量有利,而与Areao相关的环境对粮食产量不利。然而,Anhembi和Areao在丰收年更容易预测。USP 93-5082和USP 93-5243系组合具有较高的适应性和稳定性。
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引用次数: 16
Introgression of Co-4 2 and Co-5 anthracnose resistance genes into 'Carioca' Common bean cultivars Co-4 - 2和Co-5抗炭疽病基因在“Carioca”普通豆品种中的渗入
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2004-12-31 DOI: 10.12702/1984-7033.V04N04A11
A. L. Alzate-Marin, K. Arruda, K. A. Souza, E. G. Barros, M. Moreira
2 and Co-5 genes derived from G 2333, with the marker assisted selection (MAS), and b) the characterization of the resistant lines with different pathotypes of C. lindemuthianum. Thirteen lines of the 'carioca grain type' were selected; ten carrying the Co-4 2 and three the Co-5 gene, all showing resistance to the anthracnose pathotypes tested in this work. The association of adapted lines with the Co-4 2 gene combined with other resistance genes should provide durable resistance to this disease in Brazil and in other parts of the world.
2和Co-5基因来源于g2333,利用标记辅助选择(MAS)对不同病原菌抗性品系进行鉴定。“卡里奥卡粒型”选育了13个品系;10株携带Co-4 - 2基因,3株携带Co-5基因,均表现出对炭疽病的抗性。具有co - 42基因的适应品系与其他抗性基因结合,应在巴西和世界其他地区提供对该疾病的持久抗性。
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引用次数: 7
Influence of the environmental index on the estimation of stability parameters of Eberhart and Russell 环境指数对Eberhart和Russell稳定性参数估计的影响
IF 1.5 4区 农林科学 Q3 AGRONOMY Pub Date : 2004-12-31 DOI: 10.12702/1984-7033.V04N04A15
C. Pacheco, A. C. Oliveira, E. E. Gama, Manoel Xavier dos Santos, S. N. Parentoni, W. F. Meirelles, P. E. Guimarães, P. H. Ribeiro
The objective of this study was to verify the influence of the environmental index on the analysis and estimates of adaptability and stability parameters, using environmental indices obtained from four genotype groups (A, B, C, and QPM). Data of preliminary maize cultivars trials of Embrapa Maize and Sorghum, were used obtained in the 2000/2001 harvest season in nine environments of the southeast and central-western regions. The interchange of environmental indices did not affect the paramete r estimates and the genotype classification of the four groups equally in relation to their stabilities of yield and response to environments. The cause of this variation is probably more linked to the experimental errors and the differences in the interaction of the g enotypes of each group with the environments than to the covariance of the genotype means with their respective environmental indices.
本研究利用A、B、C和QPM 4个基因型组的环境指数,验证环境指数对适应性和稳定性参数分析和估计的影响。利用2000/2001年收获季在中国东南和中西部地区9个环境下获得的玉米品种初步试验数据,对巴西玉米和高粱进行初步试验。在产量稳定性和对环境的响应方面,环境指标的交换对4个类群的参数估计和基因型分类影响不大。这种变异的原因可能更多地与实验误差和每组g型与环境相互作用的差异有关,而不是与基因型均值与各自环境指数的协方差有关。
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引用次数: 0
期刊
Crop Breeding and Applied Biotechnology
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