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Induction of subject-ventilator asynchrony by variation of respiratory parameters in a lung injury model in pigs. 在猪肺损伤模型中通过呼吸参数变化诱导主体与呼吸机不同步。
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-10-03 DOI: 10.1186/s12931-024-02984-y
Xi Ran, Martin Scharffenberg, Jakob Wittenstein, Mark Leidermann, Andreas Güldner, Thea Koch, Marcelo Gama de Abreu, Robert Huhle

Background: Subject-ventilator asynchrony (SVA) was shown to be associated with negative clinical outcomes. To elucidate pathophysiology pathways and effects of SVA on lung tissue histology a reproducible animal model of artificially induced asynchrony was developed and evaluated.

Methods: Alterations in ventilator parameters were used to induce the three main types of asynchrony: ineffective efforts (IE), auto-triggering (AT), and double-triggering (DT). Airway flow and pressure, as well as oesophageal pressure waveforms, were recorded, asynchrony cycles were manually classified and the asynchrony index (AIX) was calculated. Bench tests were conducted on an active lung simulator with ventilator settings altered cycle by cycle. The developed algorithm was evaluated in three pilot experiments and a study in pigs ventilated for twelve hours with AIX = 25%.

Results: IE and AT were induced reliably and fail-safe by end-expiratory hold and adjustment of respiratory rate, respectively. DT was provoked using airway pressure ramp prolongation, however not controlled specifically in the pilots. In the subsequent study, an AIX = 28.8% [24.0%-34.4%] was induced and maintained over twelve hours.

Conclusions: The method allows to reproducibly induce and maintain three clinically relevant types of SVA observed in ventilated patients and may thus serve as a useful tool for future investigations on cellular and inflammatory effects of asynchrony.

背景:研究表明,受试者与呼吸机不同步(SVA)与不良临床结果有关。为了阐明病理生理学途径以及 SVA 对肺组织学的影响,我们开发并评估了一种可重复的人工诱导不同步动物模型:呼吸机参数的改变被用来诱导三种主要类型的不同步:无效努力(IE)、自动触发(AT)和双重触发(DT)。记录气道流量和压力以及食道压力波形,对不同步周期进行人工分类,并计算不同步指数(AIX)。在主动肺模拟器上进行了台架测试,呼吸机设置逐周期改变。在三项试点实验中对所开发的算法进行了评估,并在 AIX = 25% 的情况下对通气 12 小时的猪进行了研究:结果:分别通过呼气末屏气和调整呼吸频率诱发了 IE 和 AT,结果可靠且无故障。气道压力斜坡延长可诱发 DT,但在飞行员中没有得到特别控制。在随后的研究中,AIX=28.8%[24.0%-34.4%]被诱导并维持了12个小时:该方法可重复诱导并维持在通气患者身上观察到的三种临床相关类型的 SVA,因此可作为未来研究异步对细胞和炎症影响的有用工具。
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引用次数: 0
CircSSR1 regulates pyroptosis of pulmonary artery smooth muscle cells through parental protein SSR1 mediating endoplasmic reticulum stress. CircSSR1通过亲代蛋白SSR1介导内质网应激,调节肺动脉平滑肌细胞的热凋亡。
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-10-01 DOI: 10.1186/s12931-024-02986-w
Xiaoyu Guan, Hongxia Du, Xiaoying Wang, Xiangrui Zhu, Cui Ma, Lixin Zhang, Siyu He, June Bai, Huiyu Liu, Hao Yuan, Shanshan Wang, Kuiyu Wan, Hang Yu, Daling Zhu

Introduction: Pyroptosis, inflammatory necrosis of cells, is a programmed cell death involved in the pathological process of diseases. Endoplasmic reticulum stress (ERS), as a protective stress response of cell, decreases the unfold protein concentration to inhibit the unfold protein agglutination. Whereas the relationship between endoplasmic reticulum stress and pyroptosis in pulmonary hypertension (PH) remain unknown. Previous evident indicated that circular RNA (circRNA) can participate in several biological process, including cell pyroptosis. However, the mechanism of circRNA regulate pyroptosis of pulmonary artery smooth muscle cells through endoplasmic reticulum stress still unclear. Here, we proved that circSSR1 was down-regulate expression during hypoxia in pulmonary artery smooth muscle cells, and over-expression of circSSR1 inhibit pyroptosis both in vitro and in vivo under hypoxic. Our experiments have indicated that circSSR1 could promote host gene SSR1 translation via m6A to activate ERS leading to pulmonary artery smooth muscle cell pyroptosis. In addition, our results showed that G3BP1 as upstream regulator mediate the expression of circSSR1 under hypoxia. These results highlight a new regulatory mechanism for pyroptosis and provide a potential therapy target for pulmonary hypertension.

Methods: RNA-FISH and qRT-PCR were showed the location of circSSR1 and expression change. RNA pull-down and RIP verify the circSSR1 combine with YTHDF1. Western blotting, PI staining and LDH release were used to explore the role of circSSR1 in PASMCs pyroptosis.

Results: CircSSR1 was markedly downregulated in hypoxic PASMCs. Knockdown CircSSR1 inhibited hypoxia induced PASMCs pyroptosis in vivo and in vitro. Mechanistically, circSSR1 combine with YTHDF1 to promote SSR1 protein translation rely on m6A, activating pyroptosis via endoplasmic reticulum stress. Furthermore, G3BP1 induce circSSR1 degradation under hypoxic.

Conclusion: Our findings clarify the role of circSSR1 up-regulated parental protein SSR1 expression mediate endoplasmic reticulum stress leading to pyroptosis in PASMCs, ultimately promoting the development of pulmonary hypertension.

简介细胞凋亡(Pyroptosis),即细胞的炎性坏死,是一种程序性细胞死亡,参与疾病的病理过程。内质网应激(ERS)作为细胞的一种保护性应激反应,可降低未折叠蛋白浓度,抑制未折叠蛋白凝集。而肺动脉高压(PH)中内质网应激与热蛋白沉积之间的关系尚不清楚。以往的研究表明,环状核糖核酸(circRNA)可参与多个生物过程,包括细胞的热解。然而,circRNA通过内质网应激调控肺动脉平滑肌细胞热凋亡的机制仍不清楚。在这里,我们证实了circSSR1在缺氧时会在肺动脉平滑肌细胞中下调表达,而在体外和体内缺氧条件下,circSSR1的过度表达都会抑制细胞的析热。我们的实验表明,circSSR1可通过m6A促进宿主基因SSR1的翻译,从而激活ERS,导致肺动脉平滑肌细胞发生热休克。此外,我们的研究结果表明,G3BP1作为上游调控因子在缺氧条件下介导了circSSR1的表达。这些结果凸显了一种新的热凋亡调控机制,并为肺动脉高压提供了一个潜在的治疗靶点:RNA-FISH和qRT-PCR显示了circSSR1的位置和表达变化。RNA pull-down 和 RIP 验证了 circSSR1 与 YTHDF1 的结合。用 Western 印迹、PI 染色和 LDH 释放来探讨 circSSR1 在 PASMCs 热休克中的作用:结果:CircSSR1在缺氧的PASMCs中明显下调。结果:CircSSR1在缺氧的PASMCs中明显下调,敲除CircSSR1可抑制体内和体外缺氧诱导的PASMCs热凋亡。从机制上看,circSSR1与YTHDF1结合,依靠m6A促进SSR1蛋白翻译,通过内质网应激激活热凋亡。此外,在缺氧条件下,G3BP1诱导circSSR1降解:我们的研究结果阐明了circSSR1上调亲代蛋白SSR1表达介导内质网应激导致PASMCs热凋亡,最终促进肺动脉高压发展的作用。
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引用次数: 0
Radiomics parameters of epicardial adipose tissue predict mortality in acute pulmonary embolism. 心外膜脂肪组织的放射组学参数可预测急性肺栓塞的死亡率。
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-10-01 DOI: 10.1186/s12931-024-02977-x
Alexey Surov, Silke Zimmermann, Mattes Hinnerichs, Hans-Jonas Meyer, Anar Aghayev, Jan Borggrefe

Background: Accurate prediction of short-term mortality in acute pulmonary embolism (APE) is very important. The aim of the present study was to analyze the prognostic role of radiomics values of epicardial adipose tissue (EAT) in APE.

Methods: Overall, 508 patients were included into the study, 209 female (42.1%), mean age, 64.7 ± 14.8 years. 4.6%and 12.4% died (7- and 30-day mortality, respectively). For external validation, a cohort of 186 patients was further analysed. 20.2% and 27.7% died (7- and 30-day mortality, respectively). CTPA was performed at admission for every patient before any previous treatment on multi-slice CT scanners. A trained radiologist, blinded to patient outcomes, semiautomatically segmented the EAT on a dedicated workstation using ImageJ software. Extraction of radiomic features was applied using the pyradiomics library. After correction for correlation among features and feature cleansing by random forest and feature ranking, we implemented feature signatures using 247 features of each patient. In total, 26 feature combinations with different feature class combinations were identified. Patients were randomly assigned to a training and a validation cohort with a ratio of 7:3. We characterized two models (30-day and 7-day mortality). The models incorporate a combination of 13 features of seven different image feature classes.

Findings: We fitted the characterized models to a validation cohort (n = 169) in order to test accuracy of our models. We observed an AUC of 0.776 (CI 0.671-0.881) and an AUC of 0.724 (CI 0.628-0.820) for the prediction of 30-day mortality and 7-day mortality, respectively. The overall percentage of correct prediction in this regard was 88% and 79% in the validation cohorts. Lastly, the AUC in an independent external validation cohort was 0.721 (CI 0.633-0.808) and 0.750 (CI 0.657-0.842), respectively.

Interpretation: Radiomics parameters of EAT are strongly associated with mortality in patients with APE.

Clinical trial number: Not applicable.

背景:准确预测急性肺栓塞(APE)的短期死亡率非常重要。本研究旨在分析心外膜脂肪组织(EAT)放射组学值在急性肺栓塞中的预后作用:研究共纳入 508 例患者,其中女性 209 例(42.1%),平均年龄(64.7 ± 14.8)岁。4.6%和12.4%的患者死亡(分别为7天和30天死亡率)。为了进行外部验证,进一步分析了 186 名患者。分别有 20.2% 和 27.7% 的患者死亡(7 天和 30 天死亡率)。每位患者在入院时都要进行 CTPA,然后再使用多层 CT 扫描仪进行治疗。一名训练有素的放射科医生在对患者结果保密的情况下,在专用工作站上使用 ImageJ 软件对 EAT 进行半自动分割。使用放射组学库提取放射组学特征。通过随机森林和特征排序对特征间的相关性和特征净化进行校正后,我们使用每位患者的 247 个特征建立了特征签名。总共确定了 26 种具有不同特征类别组合的特征组合。患者以 7:3 的比例被随机分配到训练队列和验证队列中。我们建立了两个模型(30 天死亡率和 7 天死亡率)。这些模型结合了七个不同图像特征类别的 13 个特征:我们将特征模型与验证队列(n = 169)进行了拟合,以检验模型的准确性。我们观察到,预测 30 天死亡率和 7 天死亡率的 AUC 分别为 0.776(CI 0.671-0.881)和 0.724(CI 0.628-0.820)。在验证队列中,这方面的总体预测正确率分别为 88% 和 79%。最后,独立外部验证队列的AUC分别为0.721(CI 0.633-0.808)和0.750(CI 0.657-0.842):EAT的放射组学参数与APE患者的死亡率密切相关:临床试验编号:不适用。
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引用次数: 0
Extracorporeal membrane oxygenation as a bridge to lung transplantation: 5-year outcomes and bridge to decision in a large, older cohort. 体外膜肺氧合作为肺移植的桥梁:大型老年队列中的 5 年疗效和决定桥梁。
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-09-28 DOI: 10.1186/s12931-024-02968-y
Jared A Daar, Yoshiya Toyoda, Norihisa Shigemura, Sean M Baskin, Parag Desai, Matthew Gordon

Background: Extracorporeal membrane oxygenation (ECMO) as a bridge to lung transplantation (BTT) has expanded considerably, though evidence-based selection criteria and long-term outcome data are lacking. The purpose of this study was to evaluate whether risk factors often used to exclude patients from ECMO BTT-specifically older age and not yet being listed for transplant-are validated by long-term outcomes.

Methods: To ensure minimum 5-year follow-up, a retrospective cohort study was performed of adult patients actively listed for lung transplantation at a high-volume center and bridged on ECMO between January 2012 and December 2017. Data was collected through January 1, 2023.

Results: Among 50 patients bridged on ECMO, 25 survived to transplant. Median age at listing was 58 (interquartile range [IQR], 42-65) in the transplanted group and 65 (IQR, 56.5-69) in the deceased group (P = 0.051). One-year, 3-year, and 5-year survival were 88% (22/25), 60% (15/25), and 44% (11/25), respectively, with eight patients still living at the time of review. Median time spent at home during the year post-transplant was 340 days (IQR, 314-355). Older age at listing was a negative predictor of survival on ECMO to transplant (odds ratio 0.92 [95% confidence interval, 0.86-0.99], P = 0.01). Thirteen patients were placed on ECMO prior to being listed and three were listed the same day as ECMO cannulation, with 10/16 transplanted. No significant difference in post-transplant survival was found between patients placed on ECMO prior to listing (n = 10) and those already listed (n = 15) (P = 0.93, log-rank). Serial post-transplant spirometry up to 5 years and surveillance transbronchial biopsy demonstrated good allograft function and low rates of cellular rejection.

Conclusions: In one of the oldest cohorts of ECMO BTT patients described, favorable survival outcomes and allograft function were observed up to 5 years irrespective of whether patients were previously listed or bridged to decision. Despite inherent limitations to this retrospective, single-center study, the data presented support the feasibility of ECMO BTT in older and not previously listed advanced lung disease patients.

背景:体外膜肺氧合(ECMO)作为肺移植(BTT)的桥梁已大大扩展,但缺乏循证选择标准和长期结果数据。本研究的目的是评估通常用于将患者排除在 ECMO BTT 之外的风险因素--特别是年龄较大和尚未列入移植名单--是否得到长期结果的验证:为了确保至少 5 年的随访,我们对 2012 年 1 月至 2017 年 12 月期间在一家高容量中心积极列入肺移植名单并接受 ECMO 桥接的成年患者进行了一项回顾性队列研究。数据收集至 2023 年 1 月 1 日:在50名接受ECMO桥接的患者中,有25人存活至移植。移植组患者入院时的中位年龄为58岁(四分位距[IQR]为42-65岁),死亡组患者入院时的中位年龄为65岁(四分位距[IQR]为56.5-69岁)(P = 0.051)。1年、3年和5年存活率分别为88%(22/25)、60%(15/25)和44%(11/25),复查时仍有8名患者存活。移植后一年中在家度过的时间中位数为 340 天(IQR,314-355)。入院时年龄较大是 ECMO 至移植存活率的一个负向预测因素(几率比 0.92 [95% 置信区间,0.86-0.99],P = 0.01)。13 名患者在被列入名单前已接受 ECMO,3 名患者在接受 ECMO 插管的同一天被列入名单,其中 10/16 名患者接受了移植。上市前接受 ECMO 治疗的患者(10 例)与已上市的患者(15 例)在移植后存活率方面无明显差异(P=0.93,log-rank)。移植后连续 5 年的肺活量测定和经支气管活检监测显示,异体移植功能良好,细胞排斥反应发生率低:结论:在最古老的 ECMO BTT 患者队列中,无论患者之前是被列名还是桥接决定,都能观察到长达 5 年的良好生存结果和同种异体移植功能。尽管这项回顾性的单中心研究存在固有的局限性,但所提供的数据支持了在年龄较大且之前未被列入名单的晚期肺病患者中进行 ECMO BTT 的可行性。
{"title":"Extracorporeal membrane oxygenation as a bridge to lung transplantation: 5-year outcomes and bridge to decision in a large, older cohort.","authors":"Jared A Daar, Yoshiya Toyoda, Norihisa Shigemura, Sean M Baskin, Parag Desai, Matthew Gordon","doi":"10.1186/s12931-024-02968-y","DOIUrl":"https://doi.org/10.1186/s12931-024-02968-y","url":null,"abstract":"<p><strong>Background: </strong>Extracorporeal membrane oxygenation (ECMO) as a bridge to lung transplantation (BTT) has expanded considerably, though evidence-based selection criteria and long-term outcome data are lacking. The purpose of this study was to evaluate whether risk factors often used to exclude patients from ECMO BTT-specifically older age and not yet being listed for transplant-are validated by long-term outcomes.</p><p><strong>Methods: </strong>To ensure minimum 5-year follow-up, a retrospective cohort study was performed of adult patients actively listed for lung transplantation at a high-volume center and bridged on ECMO between January 2012 and December 2017. Data was collected through January 1, 2023.</p><p><strong>Results: </strong>Among 50 patients bridged on ECMO, 25 survived to transplant. Median age at listing was 58 (interquartile range [IQR], 42-65) in the transplanted group and 65 (IQR, 56.5-69) in the deceased group (P = 0.051). One-year, 3-year, and 5-year survival were 88% (22/25), 60% (15/25), and 44% (11/25), respectively, with eight patients still living at the time of review. Median time spent at home during the year post-transplant was 340 days (IQR, 314-355). Older age at listing was a negative predictor of survival on ECMO to transplant (odds ratio 0.92 [95% confidence interval, 0.86-0.99], P = 0.01). Thirteen patients were placed on ECMO prior to being listed and three were listed the same day as ECMO cannulation, with 10/16 transplanted. No significant difference in post-transplant survival was found between patients placed on ECMO prior to listing (n = 10) and those already listed (n = 15) (P = 0.93, log-rank). Serial post-transplant spirometry up to 5 years and surveillance transbronchial biopsy demonstrated good allograft function and low rates of cellular rejection.</p><p><strong>Conclusions: </strong>In one of the oldest cohorts of ECMO BTT patients described, favorable survival outcomes and allograft function were observed up to 5 years irrespective of whether patients were previously listed or bridged to decision. Despite inherent limitations to this retrospective, single-center study, the data presented support the feasibility of ECMO BTT in older and not previously listed advanced lung disease patients.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142330803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CXCL10 predicts autoimmune features and a favorable clinical course in patients with IIP: post hoc analysis of a prospective and multicenter cohort study. CXCL10可预测IIP患者的自身免疫特征和良好的临床过程:一项前瞻性多中心队列研究的事后分析。
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-09-28 DOI: 10.1186/s12931-024-02982-0
Noriyuki Enomoto, Shogo Nakai, Shusuke Yazawa, Yasutaka Mochizuka, Atsuki Fukada, Yuko Tanaka, Hyogo Naoi, Yusuke Inoue, Hideki Yasui, Masato Karayama, Yuzo Suzuki, Hironao Hozumi, Kazuki Furuhashi, Mikio Toyoshima, Masato Kono, Shiro Imokawa, Masato Fujii, Taisuke Akamatsu, Naoki Koshimizu, Koshi Yokomura, Hiroyuki Matsuda, Yusuke Kaida, Yutaro Nakamura, Masahiro Shirai, Kazutaka Mori, Masafumi Masuda, Tomoyuki Fujisawa, Naoki Inui, Hiroaki Sugiura, Hiromitsu Sumikawa, Masashi Kitani, Kazuhiro Tabata, Noriyoshi Ogawa, Takafumi Suda

Background: Interstitial pneumonia with autoimmune features (IPAF), which does not meet any of the criteria for connective tissue diseases (CTD), has been attracting an attention in patients with idiopathic interstitial pneumonia (IIP). However, the biomarkers that reflect the clinical course of these patients have not been fully elucidated.

Objective: To identify useful serum biomarkers reflecting CTD-related features and favorable prognoses in patients with IIP.

Methods: This was a post hoc analysis of a prospective and multicenter cohort study between 2015 and 2020. Newly diagnosed patients with IIP were consecutively enrolled, and 74 autoimmune features and autoantibodies were comprehensively checked during IIP diagnosis. Serum levels of CXCL10, CXCL1, CCL2, BAFF, angiopoietin-2, and leptin were evaluated at the time of IIP diagnosis.

Results: Two hundred twenty-two patients (159 men and 63 women) with IIP were enrolled. The median observation duration was 36 months. The median age was 71 years old, and median %forced vital capacity (FVC) was 84.1% at the time of IIP diagnosis. The proportion of patients who met the classification criteria for IPAF was 11.7%. In patients with high serum CXCL10, changes in both %FVC and %diffusion lung capacity for carbon monoxide at one year were significantly higher than those in patients with low CXCL10 (p = 0.014 and p = 0.009, respectively), whereas these changes were not significant for other chemokines and cytokines. High CXCL10 levels were associated with acute/subacute onset (p < 0.001) and the diagnosis of nonspecific interstitial pneumonia with organizing pneumonia overlap (p = 0.003). High CXCL10 levels were related to a higher classification of IPAF (relative risk for IPAF was 3.320, 95%CI: 1.571-7.019, p = 0.003) and lower classification of progressive pulmonary fibrosis (PPF; relative risk for PPF was 0.309, 95%CI: 0.100-0.953, p = 0.027) compared to those with low CXCL10. Finally, survival was higher in patients with IPF and high CXCL10 (p = 0.044), and high CXCL10 was a significant prognostic factor in multivariate Cox proportional hazards models (hazard ratio 0.368, p = 0.005).

Conclusions: High serum levels of CXCL10 are associated with CTD-related features, the favorable clinical course, and survival in patients with IIP, especially IPF.

Clinical trial number: Not applicable.

背景:在特发性间质性肺炎(IIP)患者中,不符合结缔组织病(CTD)标准的自身免疫性间质性肺炎(IPAF)一直备受关注。然而,反映这些患者临床病程的生物标志物尚未完全阐明:目的:确定反映特发性间质性肺炎患者 CTD 相关特征和良好预后的有用血清生物标志物:这是对2015年至2020年间一项前瞻性多中心队列研究的事后分析。新诊断的IIP患者连续入组,在IIP诊断期间全面检查了74种自身免疫特征和自身抗体。在诊断 IIP 时评估血清中 CXCL10、CXCL1、CCL2、BAFF、血管生成素-2 和瘦素的水平:共纳入 222 名 IIP 患者(男性 159 人,女性 63 人)。中位观察期为 36 个月。中位年龄为 71 岁,诊断 IIP 时的中位肺活量(FVC)为 84.1%。符合 IPAF 分类标准的患者比例为 11.7%。在血清 CXCL10 水平高的患者中,一年后一氧化碳的肺活量百分比和扩散肺活量百分比的变化均显著高于血清 CXCL10 水平低的患者(分别为 p = 0.014 和 p = 0.009),而其他趋化因子和细胞因子的变化则不显著。高水平的 CXCL10 与急性/亚急性发病有关(p 结论:高水平的 CXCL10 与急性/亚急性发病有关:高水平的血清 CXCL10 与 CTD 相关特征、良好的临床过程以及 IIP(尤其是 IPF)患者的生存率有关:临床试验编号:不适用。
{"title":"CXCL10 predicts autoimmune features and a favorable clinical course in patients with IIP: post hoc analysis of a prospective and multicenter cohort study.","authors":"Noriyuki Enomoto, Shogo Nakai, Shusuke Yazawa, Yasutaka Mochizuka, Atsuki Fukada, Yuko Tanaka, Hyogo Naoi, Yusuke Inoue, Hideki Yasui, Masato Karayama, Yuzo Suzuki, Hironao Hozumi, Kazuki Furuhashi, Mikio Toyoshima, Masato Kono, Shiro Imokawa, Masato Fujii, Taisuke Akamatsu, Naoki Koshimizu, Koshi Yokomura, Hiroyuki Matsuda, Yusuke Kaida, Yutaro Nakamura, Masahiro Shirai, Kazutaka Mori, Masafumi Masuda, Tomoyuki Fujisawa, Naoki Inui, Hiroaki Sugiura, Hiromitsu Sumikawa, Masashi Kitani, Kazuhiro Tabata, Noriyoshi Ogawa, Takafumi Suda","doi":"10.1186/s12931-024-02982-0","DOIUrl":"https://doi.org/10.1186/s12931-024-02982-0","url":null,"abstract":"<p><strong>Background: </strong>Interstitial pneumonia with autoimmune features (IPAF), which does not meet any of the criteria for connective tissue diseases (CTD), has been attracting an attention in patients with idiopathic interstitial pneumonia (IIP). However, the biomarkers that reflect the clinical course of these patients have not been fully elucidated.</p><p><strong>Objective: </strong>To identify useful serum biomarkers reflecting CTD-related features and favorable prognoses in patients with IIP.</p><p><strong>Methods: </strong>This was a post hoc analysis of a prospective and multicenter cohort study between 2015 and 2020. Newly diagnosed patients with IIP were consecutively enrolled, and 74 autoimmune features and autoantibodies were comprehensively checked during IIP diagnosis. Serum levels of CXCL10, CXCL1, CCL2, BAFF, angiopoietin-2, and leptin were evaluated at the time of IIP diagnosis.</p><p><strong>Results: </strong>Two hundred twenty-two patients (159 men and 63 women) with IIP were enrolled. The median observation duration was 36 months. The median age was 71 years old, and median %forced vital capacity (FVC) was 84.1% at the time of IIP diagnosis. The proportion of patients who met the classification criteria for IPAF was 11.7%. In patients with high serum CXCL10, changes in both %FVC and %diffusion lung capacity for carbon monoxide at one year were significantly higher than those in patients with low CXCL10 (p = 0.014 and p = 0.009, respectively), whereas these changes were not significant for other chemokines and cytokines. High CXCL10 levels were associated with acute/subacute onset (p < 0.001) and the diagnosis of nonspecific interstitial pneumonia with organizing pneumonia overlap (p = 0.003). High CXCL10 levels were related to a higher classification of IPAF (relative risk for IPAF was 3.320, 95%CI: 1.571-7.019, p = 0.003) and lower classification of progressive pulmonary fibrosis (PPF; relative risk for PPF was 0.309, 95%CI: 0.100-0.953, p = 0.027) compared to those with low CXCL10. Finally, survival was higher in patients with IPF and high CXCL10 (p = 0.044), and high CXCL10 was a significant prognostic factor in multivariate Cox proportional hazards models (hazard ratio 0.368, p = 0.005).</p><p><strong>Conclusions: </strong>High serum levels of CXCL10 are associated with CTD-related features, the favorable clinical course, and survival in patients with IIP, especially IPF.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142330801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chronic diesel exhaust exposure induced pulmonary vascular remodeling a potential trajectory for traffic related pulmonary hypertension. 慢性柴油机废气暴露诱发肺血管重塑,这是交通相关肺动脉高压的潜在轨迹。
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-09-28 DOI: 10.1186/s12931-024-02976-y
Chaohui Mu, Qinghai Li, Yong Niu, Ting Hu, Yanting Li, Tao Wang, Xinjuan Yu, Yiqiao Lv, Huiling Tang, Jing Jiang, Haibin Xu, Yuxin Zheng, Wei Han

Background: As one of the most common traffic-related pollutants, diesel exhaust (DE) confers high risk for cardiovascular and respiratory diseases. However, its impact on pulmonary vessels is still unclear.

Methods: To explore the effects of DE exposure on pulmonary vascular remodeling, our study analyzed the number and volume of small pulmonary vessels in the diesel engine testers (the DET group) from Luoyang Diesel Engine Factory and the controls (the non-DET group) from the local water company, using spirometry and carbon content in airway macrophage (CCAM) in sputum. And then we constructed a rat model of chronic DE exposure, in which 12 rats were divided into the DE group (6 rats with 16-week DE exposure) and the control group (6 rats with 16-week clean air exposure). During right heart catheterization, right ventricular systolic pressure (RVSP) was assessed by manometry. Macrophage migration inhibitory factor (MIF) in lung tissues and bronchoalveolar lavage fluid (BALF) were measured by qRT-PCR and ELISA, respectively. Histopathological analysis for cardiovascular remodeling was also performed.

Results: In DET cohort, the number and volume of small pulmonary vessels in CT were positively correlated with CCAM in sputum (P<0.05). Rat model revealed that chronic DE-exposed rats had elevated RVSP, along with increased wall thickness of pulmonary small vessels and right the ventricle. What's more, the MIF levels in BALF and lung tissues were higher in DE-exposed rats than the controls.

Conclusion: Apart from airway remodeling, DE also induces pulmonary vascular remodeling, which will lead to cardiopulmonary dysfunction.

背景:作为最常见的交通相关污染物之一,柴油废气(DE)具有引发心血管和呼吸系统疾病的高风险。然而,其对肺血管的影响仍不清楚:为了探讨柴油机废气暴露对肺血管重塑的影响,我们的研究利用肺活量测定法和痰液中的气道巨噬细胞含碳量(CCAM)分析了洛阳柴油机厂的柴油机测试者(DET组)和当地自来水公司的对照组(非DET组)的肺小血管数量和体积。然后,我们建立了慢性 DE 暴露大鼠模型,将 12 只大鼠分为 DE 组(6 只接触 DE 16 周)和对照组(6 只接触清洁空气 16 周)。在右心导管检查过程中,通过测压法评估了右心室收缩压(RVSP)。肺组织和支气管肺泡灌洗液(BALF)中的巨噬细胞迁移抑制因子(MIF)分别通过 qRT-PCR 和 ELISA 方法进行测定。此外,还对心血管重塑进行了组织病理学分析:结果:在 DET 队列中,CT 中肺部小血管的数量和体积与痰中的 CCAM 呈正相关:除气道重塑外,DE 还会诱发肺血管重塑,从而导致心肺功能障碍。
{"title":"Chronic diesel exhaust exposure induced pulmonary vascular remodeling a potential trajectory for traffic related pulmonary hypertension.","authors":"Chaohui Mu, Qinghai Li, Yong Niu, Ting Hu, Yanting Li, Tao Wang, Xinjuan Yu, Yiqiao Lv, Huiling Tang, Jing Jiang, Haibin Xu, Yuxin Zheng, Wei Han","doi":"10.1186/s12931-024-02976-y","DOIUrl":"https://doi.org/10.1186/s12931-024-02976-y","url":null,"abstract":"<p><strong>Background: </strong>As one of the most common traffic-related pollutants, diesel exhaust (DE) confers high risk for cardiovascular and respiratory diseases. However, its impact on pulmonary vessels is still unclear.</p><p><strong>Methods: </strong>To explore the effects of DE exposure on pulmonary vascular remodeling, our study analyzed the number and volume of small pulmonary vessels in the diesel engine testers (the DET group) from Luoyang Diesel Engine Factory and the controls (the non-DET group) from the local water company, using spirometry and carbon content in airway macrophage (CCAM) in sputum. And then we constructed a rat model of chronic DE exposure, in which 12 rats were divided into the DE group (6 rats with 16-week DE exposure) and the control group (6 rats with 16-week clean air exposure). During right heart catheterization, right ventricular systolic pressure (RVSP) was assessed by manometry. Macrophage migration inhibitory factor (MIF) in lung tissues and bronchoalveolar lavage fluid (BALF) were measured by qRT-PCR and ELISA, respectively. Histopathological analysis for cardiovascular remodeling was also performed.</p><p><strong>Results: </strong>In DET cohort, the number and volume of small pulmonary vessels in CT were positively correlated with CCAM in sputum (P<0.05). Rat model revealed that chronic DE-exposed rats had elevated RVSP, along with increased wall thickness of pulmonary small vessels and right the ventricle. What's more, the MIF levels in BALF and lung tissues were higher in DE-exposed rats than the controls.</p><p><strong>Conclusion: </strong>Apart from airway remodeling, DE also induces pulmonary vascular remodeling, which will lead to cardiopulmonary dysfunction.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142330799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin-mediated enhancement of lung barrier function in rats with high-altitude-associated acute lung injury via inhibition of inflammatory response. 姜黄素通过抑制炎症反应增强高海拔急性肺损伤大鼠的肺屏障功能
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-09-28 DOI: 10.1186/s12931-024-02975-z
Xinyue Yang, Jiajia Li, Yan Ma, Xiang Dong, Jinquan Qu, Feixing Liang, Jiangwei Liu

Background: Exposure to a hypobaric hypoxic environment at high altitudes can lead to lung injury. In this study, we aimed to determine whether curcumin (Cur) could improve lung barrier function and protect against high-altitude-associated acute lung injury.

Methods: Two hundred healthy rats were randomly divided into standard control, high-altitude control (HC), salidroside (40 mg/kg, positive control), and Cur (200 mg/kg) groups. Each group was further divided into five subgroups. Basic vital signs, lung injury histopathology, routine blood parameters, plasma lactate level, and arterial blood gas indicators were evaluated. Protein and inflammatory factor (tumor necrosis factor α (TNF-α), interleukin [IL]-1β, IL-6, and IL-10) concentrations in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method and enzyme-linked immunosorbent assay, respectively. Inflammation-related and lung barrier function-related proteins were analyzed using immunoblotting.

Results: Cur improved blood routine indicators such as hemoglobin and hematocrit and reduced the BALF protein content and TNF-α, IL-1β, and IL-6 levels compared with those in the HC group. It increased IL-10 levels and reduced pulmonary capillary congestion, alveolar hemorrhage, and the degree of pulmonary interstitial edema. It increased oxygen partial pressure, oxygen saturation, carbonic acid hydrogen radical, and base excess levels, and the expression of zonula occludens 1, occludin, claudin-4, and reduced carbon dioxide partial pressure, plasma lactic acid, and the expression of phospho-nuclear factor kappa.

Conclusions: Exposure to a high-altitude environment for 48 h resulted in severe lung injury in rats. Cur improved lung barrier function and alleviated acute lung injury in rats at high altitudes.

背景:暴露在高海拔地区的低压缺氧环境中会导致肺损伤。在本研究中,我们旨在确定姜黄素(Cur)是否能改善肺屏障功能并保护肺免受高海拔相关急性肺损伤:方法:将 200 只健康大鼠随机分为标准对照组、高海拔对照组(HC)、沙利度苷(40 毫克/千克,阳性对照)和姜黄素(200 毫克/千克)组。每组又分为五个亚组。对基本生命体征、肺损伤组织病理学、血常规参数、血浆乳酸水平和动脉血气指标进行评估。支气管肺泡灌洗液(BALF)中的蛋白质和炎症因子(肿瘤坏死因子α(TNF-α)、白细胞介素[IL]-1β、IL-6和IL-10)浓度分别用双喹啉酸法和酶联免疫吸附法测定。免疫印迹法分析了炎症相关蛋白和肺屏障功能相关蛋白:结果:与 HC 组相比,Cur 改善了血红蛋白和血细胞比容等血常规指标,降低了 BALF 蛋白含量和 TNF-α、IL-1β、IL-6 水平。它提高了 IL-10 水平,减轻了肺毛细血管充血、肺泡出血和肺间质水肿程度。它提高了氧分压、血氧饱和度、碳酸氢根、碱过量水平以及闭塞带 1、闭塞素、Claudin-4 的表达,降低了二氧化碳分压、血浆乳酸和磷酸核因子 kappa 的表达:结论:在高海拔环境中暴露 48 小时会导致大鼠肺部严重损伤。Cur能改善肺屏障功能,减轻高海拔地区大鼠的急性肺损伤。
{"title":"Curcumin-mediated enhancement of lung barrier function in rats with high-altitude-associated acute lung injury via inhibition of inflammatory response.","authors":"Xinyue Yang, Jiajia Li, Yan Ma, Xiang Dong, Jinquan Qu, Feixing Liang, Jiangwei Liu","doi":"10.1186/s12931-024-02975-z","DOIUrl":"https://doi.org/10.1186/s12931-024-02975-z","url":null,"abstract":"<p><strong>Background: </strong>Exposure to a hypobaric hypoxic environment at high altitudes can lead to lung injury. In this study, we aimed to determine whether curcumin (Cur) could improve lung barrier function and protect against high-altitude-associated acute lung injury.</p><p><strong>Methods: </strong>Two hundred healthy rats were randomly divided into standard control, high-altitude control (HC), salidroside (40 mg/kg, positive control), and Cur (200 mg/kg) groups. Each group was further divided into five subgroups. Basic vital signs, lung injury histopathology, routine blood parameters, plasma lactate level, and arterial blood gas indicators were evaluated. Protein and inflammatory factor (tumor necrosis factor α (TNF-α), interleukin [IL]-1β, IL-6, and IL-10) concentrations in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method and enzyme-linked immunosorbent assay, respectively. Inflammation-related and lung barrier function-related proteins were analyzed using immunoblotting.</p><p><strong>Results: </strong>Cur improved blood routine indicators such as hemoglobin and hematocrit and reduced the BALF protein content and TNF-α, IL-1β, and IL-6 levels compared with those in the HC group. It increased IL-10 levels and reduced pulmonary capillary congestion, alveolar hemorrhage, and the degree of pulmonary interstitial edema. It increased oxygen partial pressure, oxygen saturation, carbonic acid hydrogen radical, and base excess levels, and the expression of zonula occludens 1, occludin, claudin-4, and reduced carbon dioxide partial pressure, plasma lactic acid, and the expression of phospho-nuclear factor kappa.</p><p><strong>Conclusions: </strong>Exposure to a high-altitude environment for 48 h resulted in severe lung injury in rats. Cur improved lung barrier function and alleviated acute lung injury in rats at high altitudes.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142330800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reliability of crackles in fibrotic interstitial lung disease: a prospective, longitudinal study. 纤维化间质性肺病噼啪声的可靠性:一项前瞻性纵向研究。
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-09-28 DOI: 10.1186/s12931-024-02979-9
Giacomo Sgalla, Jacopo Simonetti, Arianna Di Bartolomeo, Tonia Magrì, Bruno Iovene, Giuliana Pasciuto, Ruben Dell'Ariccia, Francesco Varone, Alessia Comes, Paolo Maria Leone, Venere Piluso, Alessandro Perrotta, Giuseppe Cicchetti, Diana Verdirosi, Luca Richeldi

Background: Although crackles on chest auscultation represent a fundamental component of the diagnostic suspect for fibrotic interstitial lung disease (ILD), their reliability has not been properly studied. We assessed the agreement among respiratory physicians on the presence and changes over time of audible crackles collected in a prospective longitudinal cohort of patients with fibrotic ILD.

Methods: Lung sounds were digitally recorded at baseline and after 12 months at eight anatomical sites. Nine respiratory physicians blindly assessed randomized couples of recordings obtained from the same anatomical site at different timepoints. The physicians indicated the presence of crackles in individual recordings and which recording from each couple eventually had more intense crackles. Fleiss' kappa coefficient was used to measure inter- and intra-rater agreement.

Results: Fifty-two patients, mostly with a diagnosis of IPF (n = 40, 76.9%) were prospectively enrolled between October 2019 and May 2021. The final acoustic dataset included 702 single recordings, corresponding to 351 couples of recordings from baseline and 12-months timepoints. Kappa coefficient was 0.57 (95% CI 0.55-0.58) for the presence of crackles and 0.42 (95% CI 0.41-0.43) for acoustic change. Intra-rater agreement, measured for three respiratory physicians on three repeated assessments, ranged from good to excellent for the presence of crackles (κ = 0.87, κ = 0.86, κ = 0.79), and from moderate to good for acoustic change (κ = 0.75, κ = 0.76, κ = 0.57).

Conclusions: Agreement between respiratory physicians for the presence of crackles and acoustic change was acceptable, suggesting that crackles represent a reliable acoustic finding in patients with fibrotic ILD. Their role as a lung-derived indicator of disease progression merits further studies.

背景:尽管胸部听诊噼啪声是纤维化间质性肺病(ILD)疑似诊断的基本组成部分,但其可靠性尚未得到适当研究。我们评估了呼吸科医生对纤维化间质性肺病患者前瞻性纵向队列中收集到的可闻噼啪声的存在和随时间变化的一致性:方法:分别在基线和 12 个月后对 8 个解剖部位的肺音进行数字记录。九名呼吸科医生对不同时间点同一解剖部位的随机记录进行盲法评估。医生们指出单个记录中是否存在噼啪声,以及每对记录中哪个记录最终出现了更强烈的噼啪声。弗莱斯卡帕系数用于衡量评分者之间和评分者内部的一致性:在 2019 年 10 月至 2021 年 5 月期间,52 名患者进行了前瞻性登记,其中大部分被诊断为 IPF(n = 40,76.9%)。最终的声学数据集包括 702 个单次记录,对应于基线和 12 个月时间点的 351 对记录。对于是否存在噼啪声,Kappa系数为0.57(95% CI 0.55-0.58);对于声学变化,Kappa系数为0.42(95% CI 0.41-0.43)。三名呼吸科医生在三次重复评估中的评分者内部一致性从良好到优秀(κ = 0.87、κ = 0.86、κ = 0.79),声学变化从中等到良好(κ = 0.75、κ = 0.76、κ = 0.57):结论:呼吸科医生对是否存在噼啪声和声学变化的一致意见是可以接受的,这表明噼啪声是纤维化 ILD 患者可靠的声学发现。作为疾病进展的肺源性指标,噼啪声的作用值得进一步研究。
{"title":"Reliability of crackles in fibrotic interstitial lung disease: a prospective, longitudinal study.","authors":"Giacomo Sgalla, Jacopo Simonetti, Arianna Di Bartolomeo, Tonia Magrì, Bruno Iovene, Giuliana Pasciuto, Ruben Dell'Ariccia, Francesco Varone, Alessia Comes, Paolo Maria Leone, Venere Piluso, Alessandro Perrotta, Giuseppe Cicchetti, Diana Verdirosi, Luca Richeldi","doi":"10.1186/s12931-024-02979-9","DOIUrl":"https://doi.org/10.1186/s12931-024-02979-9","url":null,"abstract":"<p><strong>Background: </strong>Although crackles on chest auscultation represent a fundamental component of the diagnostic suspect for fibrotic interstitial lung disease (ILD), their reliability has not been properly studied. We assessed the agreement among respiratory physicians on the presence and changes over time of audible crackles collected in a prospective longitudinal cohort of patients with fibrotic ILD.</p><p><strong>Methods: </strong>Lung sounds were digitally recorded at baseline and after 12 months at eight anatomical sites. Nine respiratory physicians blindly assessed randomized couples of recordings obtained from the same anatomical site at different timepoints. The physicians indicated the presence of crackles in individual recordings and which recording from each couple eventually had more intense crackles. Fleiss' kappa coefficient was used to measure inter- and intra-rater agreement.</p><p><strong>Results: </strong>Fifty-two patients, mostly with a diagnosis of IPF (n = 40, 76.9%) were prospectively enrolled between October 2019 and May 2021. The final acoustic dataset included 702 single recordings, corresponding to 351 couples of recordings from baseline and 12-months timepoints. Kappa coefficient was 0.57 (95% CI 0.55-0.58) for the presence of crackles and 0.42 (95% CI 0.41-0.43) for acoustic change. Intra-rater agreement, measured for three respiratory physicians on three repeated assessments, ranged from good to excellent for the presence of crackles (κ = 0.87, κ = 0.86, κ = 0.79), and from moderate to good for acoustic change (κ = 0.75, κ = 0.76, κ = 0.57).</p><p><strong>Conclusions: </strong>Agreement between respiratory physicians for the presence of crackles and acoustic change was acceptable, suggesting that crackles represent a reliable acoustic finding in patients with fibrotic ILD. Their role as a lung-derived indicator of disease progression merits further studies.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142330813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential proteins from EVs identification based on tandem mass tags analysis and effect of Treg-derived EVs on T-lymphocytes in COPD patients. 基于串联质量标记分析的 EVs 差异蛋白质鉴定及 Treg 衍生 EVs 对 COPD 患者 T 淋巴细胞的影响
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-09-28 DOI: 10.1186/s12931-024-02980-2
Xuefang Tao, Zhisong Xu, Hai Tian, Jingfeng He, Guowen Wang, Xuexia Tao

Background: Chronic obstructive pulmonary disease (COPD) is a widespread respiratory disease. This study examines extracellular vesicles (EVs) and proteins contained in EVs in COPD.

Methods: Blood samples were collected from 40 COPD patients and 10 health controls. Cytokines including IFN-γ, TNF-α, IL-1β, IL-6, IL-8, and IL-17, were measured by ELISA. Small EVs samples were extracted from plasma and identified by transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and Western blot. Protein components contained in EVs were analyzed by Tandem Mass Tags (TMT) to identify differential proteins. Treg-derived EV was extracted and added to isolated CD8+, Treg, and Th17 subsets to assess its effect on T-lymphocytes.

Results: ELISA revealed higher levels of all cytokines and flow cytometry suggested a higher proportion of Treg and Th17 cells in COPD patients. After identification, TMT analysis identified 207 unique protein components, including five potential COPD biomarkers: BTRC, TRIM28, CD209, NCOA3, and SSR3. Flow cytometry revealed that Treg-derived EVs inhibited differentiation into CD8+, CD4+, and Th17 cells.

Conclusion: The study shows that cytokines, T-lymphocyte subsets differences in COPD and Treg-derived EVs influence T-lymphocyte differentiation. Identified biomarkers may assist in understanding COPD pathogenesis, prognosis, and therapy. The study contributes to COPD biomarker research.

背景:慢性阻塞性肺疾病(COPD)是一种广泛存在的呼吸系统疾病。本研究对慢性阻塞性肺病患者的细胞外囊泡 (EV) 和 EV 中所含的蛋白质进行了研究:方法:收集 40 名慢性阻塞性肺病患者和 10 名健康对照者的血液样本。采用 ELISA 法检测细胞因子,包括 IFN-γ、TNF-α、IL-1β、IL-6、IL-8 和 IL-17。从血浆中提取小的 EVs 样品,并通过透射电子显微镜(TEM)、纳米颗粒追踪分析(NTA)和 Western 印迹进行鉴定。利用串联质量标签(TMT)分析了EVs中的蛋白质成分,以确定不同的蛋白质。提取Treg衍生EV并将其加入分离的CD8+、Treg和Th17亚群,以评估其对T淋巴细胞的影响:结果:酶联免疫吸附试验(ELISA)显示慢性阻塞性肺病患者体内所有细胞因子的水平都较高,流式细胞术显示慢性阻塞性肺病患者体内 Treg 和 Th17 细胞的比例较高。经过鉴定,TMT 分析确定了 207 种独特的蛋白质成分,其中包括五种潜在的慢性阻塞性肺病生物标志物:BTRC、TRIM28、CD209、NCOA3 和 SSR3。流式细胞术显示,Treg衍生的EV抑制了CD8+、CD4+和Th17细胞的分化:研究表明,细胞因子、慢性阻塞性肺病的 T 淋巴细胞亚群差异以及 Treg 衍生的 EVs 会影响 T 淋巴细胞的分化。确定的生物标志物可能有助于了解慢性阻塞性肺病的发病机制、预后和治疗。该研究有助于慢性阻塞性肺病生物标志物的研究。
{"title":"Differential proteins from EVs identification based on tandem mass tags analysis and effect of Treg-derived EVs on T-lymphocytes in COPD patients.","authors":"Xuefang Tao, Zhisong Xu, Hai Tian, Jingfeng He, Guowen Wang, Xuexia Tao","doi":"10.1186/s12931-024-02980-2","DOIUrl":"https://doi.org/10.1186/s12931-024-02980-2","url":null,"abstract":"<p><strong>Background: </strong>Chronic obstructive pulmonary disease (COPD) is a widespread respiratory disease. This study examines extracellular vesicles (EVs) and proteins contained in EVs in COPD.</p><p><strong>Methods: </strong>Blood samples were collected from 40 COPD patients and 10 health controls. Cytokines including IFN-γ, TNF-α, IL-1β, IL-6, IL-8, and IL-17, were measured by ELISA. Small EVs samples were extracted from plasma and identified by transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and Western blot. Protein components contained in EVs were analyzed by Tandem Mass Tags (TMT) to identify differential proteins. Treg-derived EV was extracted and added to isolated CD8<sup>+</sup>, Treg, and Th17 subsets to assess its effect on T-lymphocytes.</p><p><strong>Results: </strong>ELISA revealed higher levels of all cytokines and flow cytometry suggested a higher proportion of Treg and Th17 cells in COPD patients. After identification, TMT analysis identified 207 unique protein components, including five potential COPD biomarkers: BTRC, TRIM28, CD209, NCOA3, and SSR3. Flow cytometry revealed that Treg-derived EVs inhibited differentiation into CD8<sup>+</sup>, CD4<sup>+</sup>, and Th17 cells.</p><p><strong>Conclusion: </strong>The study shows that cytokines, T-lymphocyte subsets differences in COPD and Treg-derived EVs influence T-lymphocyte differentiation. Identified biomarkers may assist in understanding COPD pathogenesis, prognosis, and therapy. The study contributes to COPD biomarker research.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142330802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-cell transcriptomics reveals e-cigarette vapor-induced airway epithelial remodeling and injury. 单细胞转录组学揭示了电子烟蒸汽诱导的气道上皮重塑和损伤。
IF 5.8 2区 医学 Q1 Medicine Pub Date : 2024-09-28 DOI: 10.1186/s12931-024-02962-4
Weitao Cao, Jia Li, Li Che, Ruixue Yang, Zehong Wu, Guoping Hu, Weifeng Zou, Zehang Zhao, Yumin Zhou, Xingtao Jiang, Tiejun Zhang, Wenguang Yin, Pixin Ran

Background: In recent years, e-cigarettes have been used as alternatives among adult smokers. However, the impact of e-cigarette use on human bronchial epithelial (HBE) cells remains controversial.

Methods: We collected primary HBE cells of healthy nonsmokers and chronic obstructive pulmonary disease (COPD) smokers, and analyzed the impact of e- cigarette vapor extract (ECE) or cigarette smoke extract (CSE) on HBE cell differentiation and injury by single-cell RNA sequencing, immunostaining, HE staining, qPCR and ELISA. We obtained serum and sputum from healthy non- smokers, smokers and e-cigarette users, and analyzed cell injury markers and mucin proteins.

Results: ECE treatment led to a distinct differentiation program of ciliated cells and unique patterns of their cell-cell communications compared with CSE. ECE treatment caused increased Notch signaling strength in a ciliated cell subpopulation, and HBE cell remodeling and injury including hypoplasia of ciliated cells and club cells, and shorter cilia. ECE-induced hypoplasia of ciliated cells and shorter cilia were ameliorated by the Notch signaling inhibition.

Conclusions: This study reveals distinct characteristics in e-cigarette vapor-induced airway epithelial remodeling, pointing to Notch signaling pathway as a potential targeted intervention for e-cigarette vapor-caused ciliated cell differentiation defects and cilia injury. In addition, a decrease in SCGB1A1 proteins is associated with e- cigarette users, indicating a potential lung injury marker for e-cigarette users.

背景:近年来,电子烟已成为成年吸烟者的替代品。然而,使用电子烟对人类支气管上皮细胞(HBE)的影响仍存在争议:我们收集了健康非吸烟者和慢性阻塞性肺病(COPD)吸烟者的原代 HBE 细胞,并通过单细胞 RNA 测序、免疫染色、HE 染色、qPCR 和 ELISA 分析了电子烟蒸汽提取物(ECE)或香烟烟雾提取物(CSE)对 HBE 细胞分化和损伤的影响。我们采集了健康非吸烟者、吸烟者和电子烟使用者的血清和痰液,分析了细胞损伤标记物和粘蛋白:结果:与CSE相比,ECE处理导致了纤毛细胞的独特分化程序及其独特的细胞间通讯模式。ECE 处理会导致纤毛细胞亚群的 Notch 信号强度增加,并导致 HBE 细胞重塑和损伤,包括纤毛细胞和棒状细胞减少以及纤毛变短。Notch信号抑制可改善ECE诱导的纤毛细胞减少和纤毛变短:这项研究揭示了电子烟蒸汽诱导的气道上皮重塑的独特特征,指出Notch信号通路是治疗电子烟蒸汽导致的纤毛细胞分化缺陷和纤毛损伤的潜在靶向干预措施。此外,SCGB1A1 蛋白的减少与电子烟使用者有关,这表明电子烟使用者有可能成为肺损伤标志物。
{"title":"Single-cell transcriptomics reveals e-cigarette vapor-induced airway epithelial remodeling and injury.","authors":"Weitao Cao, Jia Li, Li Che, Ruixue Yang, Zehong Wu, Guoping Hu, Weifeng Zou, Zehang Zhao, Yumin Zhou, Xingtao Jiang, Tiejun Zhang, Wenguang Yin, Pixin Ran","doi":"10.1186/s12931-024-02962-4","DOIUrl":"https://doi.org/10.1186/s12931-024-02962-4","url":null,"abstract":"<p><strong>Background: </strong>In recent years, e-cigarettes have been used as alternatives among adult smokers. However, the impact of e-cigarette use on human bronchial epithelial (HBE) cells remains controversial.</p><p><strong>Methods: </strong>We collected primary HBE cells of healthy nonsmokers and chronic obstructive pulmonary disease (COPD) smokers, and analyzed the impact of e- cigarette vapor extract (ECE) or cigarette smoke extract (CSE) on HBE cell differentiation and injury by single-cell RNA sequencing, immunostaining, HE staining, qPCR and ELISA. We obtained serum and sputum from healthy non- smokers, smokers and e-cigarette users, and analyzed cell injury markers and mucin proteins.</p><p><strong>Results: </strong>ECE treatment led to a distinct differentiation program of ciliated cells and unique patterns of their cell-cell communications compared with CSE. ECE treatment caused increased Notch signaling strength in a ciliated cell subpopulation, and HBE cell remodeling and injury including hypoplasia of ciliated cells and club cells, and shorter cilia. ECE-induced hypoplasia of ciliated cells and shorter cilia were ameliorated by the Notch signaling inhibition.</p><p><strong>Conclusions: </strong>This study reveals distinct characteristics in e-cigarette vapor-induced airway epithelial remodeling, pointing to Notch signaling pathway as a potential targeted intervention for e-cigarette vapor-caused ciliated cell differentiation defects and cilia injury. In addition, a decrease in SCGB1A1 proteins is associated with e- cigarette users, indicating a potential lung injury marker for e-cigarette users.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142330815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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