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Cyclic variation of the oviduct structure of Boulengerula taitana, an oviparous species of Gymnophiona: morphological changes, proliferation and apoptosis 裸子植物卵生种黄颡鱼(Boulengerula taitana)输卵管结构的循环变化:形态变化、增殖和凋亡
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2017-07-03 DOI: 10.1080/21564574.2017.1365098
C. Brun, Michel Raquet, G. J. Measey, J. Exbrayat
Abstract Functioning of the amphibian oviduct is the subject of many studies, but detailed understanding of caecilian reproduction exists for very few species. In this study, a description is made of morphological changes of the oviduct of the caecilian Boulengerula taitana throughout the year, using conventional histology and immunohistochemistry. The reproductive cycle of this little-known species, originating from the Taita Hills in southeast Kenya, is annual. During the reproductive period (November–February), both lamina propria and the epithelium of the oviduct exhibit a maximum degree of development. The epithelial mucosa is more secretory and the cilia cells are abundant. Histological analysis shows the distribution of the different type of cells according to a specific pattern in the pars recta, pars convoluta and pars utera. Evaluation of proliferative (Ki-67 positive cells) and apoptotic cells (TUNEL and Apostain assays) into the different tissues brings out cyclic modifications of the lamina propria and epithelial cells throughout a seasonal cycle. The results demonstrate that the balance between cell proliferation and apoptosis reflects the functionality of the oviduct during the sexual cycle.
两栖动物输卵管的功能是许多研究的主题,但对蚓类生殖的详细了解很少。本研究采用常规组织学和免疫组织化学方法,描述了一年中盲肠虫(Boulengerula taitana)输卵管的形态学变化。这种鲜为人知的物种起源于肯尼亚东南部的泰塔山,其繁殖周期是一年一次。在生殖期(11 - 2月),输卵管固有层和上皮发育程度最大。上皮粘膜分泌较多,纤毛细胞丰富。组织学分析显示,不同类型的细胞在直肠部、卷腹部和子宫部按特定的模式分布。对不同组织中的增殖细胞(Ki-67阳性细胞)和凋亡细胞(TUNEL和Apostain测定)进行评估,发现固有层和上皮细胞在整个季节周期中发生了循环修饰。结果表明,细胞增殖和凋亡之间的平衡反映了性周期中输卵管的功能。
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引用次数: 3
Genetic diversity and differentiation of the Western Leopard Toad (Sclerophrys pantherina) based on mitochondrial and microsatellite markers 基于线粒体和微卫星标记的西豹蟾蜍遗传多样性和分化
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2017-01-02 DOI: 10.1080/21564574.2017.1294115
J. M. D. Silva, K. Feldheim, G. J. Measey, Stephen Doucette-Riise, R. Daniels, L. Chauke, K. Tolley
Abstract Intraspecific genetic diversity provides the basis for evolutionary change and is therefore considered the most fundamental level of biodiversity. Mitochondrial DNA (mtDNA) and microsatellite loci are the markers most typically used in population-level studies; however, their patterns of genetic variation are not always congruent. This can result in different interpretations of the data, which can impact on management decisions, especially for threatened species. Consequently, in this study, we developed and analysed novel microsatellite markers for the Endangered Western Leopard Toad (WLT), Sclerophrys pantherina, and compared the results to previously published mtDNA data to compare the level of genetic diversity between the two molecular markers. The microsatellite evidence showed signs of a past bottleneck, yet relatively high levels of genetic diversity and low genetic differentiation between two sampling sites. In contrast, the mtDNA revealed moderate to low levels of diversity between sampling sites, and strong genetic differentiation. An explanation for the conflicting patterns may be that the current genetic signature, as depicted by the microsatellite data, is not yet reflected in the mitochondrial dataset; and, as such the data are depicting a timeline for genetic variation within the WLT. Both markers revealed important information about the two sampling sites, which can help inform conservation management of the species.
摘要种内遗传多样性为进化变化提供了基础,因此被认为是生物多样性的最基本水平。线粒体DNA(mtDNA)和微卫星基因座是群体水平研究中最常用的标记;然而,它们的遗传变异模式并不总是一致的。这可能导致对数据的不同解释,从而影响管理决策,尤其是对受威胁物种。因此,在这项研究中,我们开发并分析了濒危西部豹蟾蜍(WLT)的新微卫星标记,即泛盘硬眼蟾蜍,并将结果与先前发表的mtDNA数据进行了比较,以比较两个分子标记之间的遗传多样性水平。微卫星证据显示出过去存在瓶颈的迹象,但两个采样点之间的遗传多样性水平相对较高,遗传分化程度较低。相比之下,mtDNA显示采样点之间的多样性水平中等至较低,遗传分化强烈。对冲突模式的解释可能是,微卫星数据所描述的当前遗传特征尚未反映在线粒体数据集中;因此,数据描绘了WLT内遗传变异的时间线。这两个标记都揭示了关于这两个采样点的重要信息,这有助于为该物种的保护管理提供信息。
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引用次数: 4
Erythrocyte morphology of the Critically Endangered Geometric Tortoise Psammobates geometricus: effects of season, sex and age 极度濒危几何龟红细胞形态:季节、性别和年龄的影响
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2017-01-02 DOI: 10.1080/21564574.2017.1300610
S. Walton, M. Hofmeyr
Abstract Land transformation reduced the habitat of Psammobates geometricus to small isolated fragments, rendering the species vulnerable to extinction. Field biologists often use erythrocyte metrics to assess wildlife health but need baseline values to distinguish normal variations from disease or chronic stress. Here we characterise erythrocyte types and features of P. geometricus and assess if age, sex and environmental fluctuations influence their frequencies and metrics. We collected blood from males, females and juveniles over four seasons to prepare smears for cytological evaluation. We treated the smears with May–Grünwald–Giemsa stains and used imaging software to measure size, shape and colour intensity of erythrocyte types. The blood of all groups contained immature, mature and senescent erythrocytes, and the appearance of each cell type corresponded to descriptions for other chelonians. Season had a strong effect on the occurrence of erythrocyte types and features with few group differences. The frequency of immature erythrocytes was high in winter and spring, the wet season, reflecting increased erythropoiesis. Mature erythrocytes dominated in summer and autumn, but frequencies of senescent cells, poikilocytosis, cytoplasmic inclusions and cytoplasmic vacuoles increased in autumn, indicating that water and food shortages caused physiological stress. The small size and elongated shape of summer and autumn erythrocytes would facilitate oxygen exchange when high temperatures increase metabolic needs of ectotherms. Lower temperatures and energy demands in the wet season counter the lower oxygen capacity of round, immature cells. Our results provide baseline information on erythrocyte physiology and metrics for health assessments of geometric tortoises.
摘要土地改造使Psammobates geomicus的栖息地变成了孤立的小碎片,使该物种容易灭绝。野外生物学家经常使用红细胞指标来评估野生动物的健康状况,但需要基线值来区分正常变化与疾病或慢性压力。在这里,我们描述了P.geomicus的红细胞类型和特征,并评估年龄、性别和环境波动是否会影响其频率和指标。我们收集了四个季节的雄性、雌性和幼年动物的血液,准备进行细胞学评估的涂片。我们用May–Grünwald–Giemsa染色剂处理涂片,并使用成像软件测量红细胞类型的大小、形状和颜色强度。所有组的血液中都含有未成熟、成熟和衰老的红细胞,每种细胞类型的外观都与其他螯合物的描述相对应。季节对红细胞类型和特征的发生有很大影响,但组间差异不大。未成熟红细胞的频率在冬春雨季较高,反映红细胞生成增加。成熟红细胞在夏季和秋季占主导地位,但衰老细胞、红细胞增多、细胞质内含物和细胞质液泡的频率在秋季增加,表明缺水和食物短缺造成了生理压力。当高温增加外胚层的代谢需求时,夏季和秋季红细胞的小体积和细长形状将促进氧气交换。雨季较低的温度和能量需求抵消了圆形未成熟细胞较低的氧气容量。我们的研究结果为几何陆龟的健康评估提供了红细胞生理学和指标的基线信息。
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引用次数: 1
Daily activity, biometry and diet of the North African ocellated lizard Timon pater in Mount Chélia, north-eastern Algeria 阿尔及利亚东北部契姆利亚山北非斑点蜥蜴的日常活动、生物特征和饮食
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2017-01-02 DOI: 10.1080/21564574.2016.1271834
Messaoud Saoudi, A. Necer, Idriss Bouam, Farouk Khelfaoui, Oussama Saadi
Abstract Although Timon pater is among the largest species of lacertids found throughout North Africa, mainly in Morocco, Algeria and Tunisia, studies examining the ecology of this lizard remain rare. In this paper we describe the daily activity patterns, biometry and diet of T. pater in a mountain area during its period of maximum activity. We carried out the field study in the southern slope of Mount Chélia in the middle of the Aurès Massif, north-eastern Algeria. In order to analyse the daily activity patterns we performed surveys along 4 000 m long random transects. To obtain biometrical measurements we captured and measured 20 adults with a digital calliper. We obtained diet data by analysing 17 extracted stomach contents. The daily activity patterns of T. pater were similar during the summer periods (early and late summer), and no variation in activity was detected. Adult males were slightly larger and heavier than adult females and their heads were longer, larger and thicker than females’ heads. T. pater primarily consumed Coleopterans, but diet composition was noticeably different between the two summer periods. There were no sexual differences in dietary preferences.
摘要尽管蒂蒙·帕特尔是北非发现的最大的草类之一,主要分布在摩洛哥、阿尔及利亚和突尼斯,但对这种蜥蜴生态的研究仍然很少。本文描述了山区T.pater在其最大活动期的日常活动模式、生物测量和饮食。我们在阿尔及利亚东北部Aurès Massif中部的Chélia山南坡进行了实地研究。为了分析日常活动模式,我们沿着4 000m长的随机横断面。为了获得生物特征测量,我们用数字卡尺捕获并测量了20名成年人。我们通过分析17份提取的胃内容物获得了饮食数据。在夏季(夏初和夏末),T.pater的日常活动模式相似,没有检测到活动的变化。成年雄性比成年雌性稍大、稍重,它们的头部比雌性的头部更长、更大、更厚。T.pater主要食用鞘翅目昆虫,但两个夏季的饮食组成明显不同。饮食偏好没有性别差异。
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引用次数: 1
Three new species of Arthroleptella Hewitt, 1926 (Anura: Pyxicephalidae) from the Cape Fold Mountains, South Africa 休伊特节肢动物三新种,1926(无尾目:大头虱科),南非福尔德角山
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2017-01-02 DOI: 10.1080/21564574.2017.1324918
A. Turner, A. Channing
Abstract A comprehensive survey of the Cape Fold Mountains, South Africa, was undertaken for moss frogs of the genus Arthroleptella. Advertisement calls were recorded and voucher specimens collected for all populations encountered. A molecular phylogeny was constructed for the entire genus using two mitochondrial and one nuclear marker. Standard morphological measurements were made and salient morphological characters described. Analysis of these data revealed the presence of three undescribed species: two that were previously obscured under Arthroleptella bicolor and one obscured under A. landdrosia are described herein. The advertisement calls of the three new species are described and contrasted with the calls of other Arthroleptella species. Their distribution is described and spatial extent of occurrence quantified along with a brief discussion of conservation status for each species. ZooBank.—Arthroleptella draconella: urn:lsid:zoobank.org:act:341BABC2-63A8-4C60-A306-D232E92ECE69 Arthroleptella atermina: urn:lsid:zoobank.org:act:AC23D804-3075-40DB-9852-624C32A51547 Arthroleptella kogelbergensis: urn:lsid:zoobank.org:act:AA573B06-BA81-4B84-B436-5F5CCDF1D892.
摘要对南非福尔德角山脉的节肢动物属苔藓蛙进行了全面调查。记录了广告电话,并收集了遇到的所有人群的优惠券样本。利用两个线粒体和一个核标记构建了整个属的分子系统发育。进行了标准形态学测量,并描述了显著的形态学特征。对这些数据的分析揭示了三个未描述的物种的存在:两个以前在双色节翅目下被遮蔽,一个在兰德龙下被遮蔽。对这三个新种的广告叫声进行了描述,并与其他节肢动物的叫声进行了对比。描述了它们的分布,量化了它们的发生空间范围,并简要讨论了每个物种的保护状况。ZooBank--龙舌兰:urn:lsid:zoobank.org:act:341BABC2-63A8-4C60-A306-D232E92ECE69龙舌兰atermina:urn:lsid:zooback.org:act:AC23D804-3075-40DB-9852-624C32A51547科格尔伯格节叶兰:urn:sid:zoebank.org:aact:AA573B06-BA81-4B84-B436-5F5CCDF1D892。
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引用次数: 8
Sheddable armour: identification of osteoderms in the integument of Geckolepis maculata (Gekkota) 脱落甲:斑点壁虎(Gekkota)表皮骨皮的鉴定
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2017-01-02 DOI: 10.1080/21564574.2017.1281172
Daniel J. Paluh, Aaron H. Griffing, A. Bauer
Abstract Osteoderms are bony deposits that form within the dermal layer of skin in vertebrates. Within geckos, osteoderms are uncommon, only described in Gekko gecko and the genus Tarentola. A previous report of osteoderms in the Afro-Malagasy gekkonid genus Geckolepis has been questioned due to the extreme skin fragility and regional integumentary loss within the group. We re-evaluated the integument anatomy of Geckolepis maculata using µCT scanning, histology, and clearing and staining to verify the presence or absence of osteoderms and, if present, to characterise and compare their morphology to the osteoderms of Gekko gecko and Tarentola, as well as osteoderm diversity within squamates. Osteoderms were confirmed present in Geckolepis maculata; however, these osteoderms are compound, imbricating, squamous elements that are morphologically similar to the dermal ossifications observed in scincids and gerrhosaurids, while G. gecko and Tarentola possess plate-like and granular osteoderms. Our results suggest that osteoderms have independently evolved at least three times within the Gekkota and that epidermal anatomy may be a better predictor of osteoderm morphology than ancestral history. Further research is required to investigate the regenerative capability of Geckolepis osteoderms following skin autotomy.
摘要骨皮动物是脊椎动物皮肤真皮层内形成的骨沉积物。在壁虎中,骨皮病并不常见,只有Gekko壁虎和Tarentola属才有记载。此前一份关于非洲-马达加斯加壁虎属骨皮病的报告受到质疑,因为该属的皮肤极度脆弱,局部表皮缺失。我们使用µCT扫描、组织学、清除和染色重新评估了斑点壁虎的表皮解剖结构,以验证骨皮的存在或不存在,如果存在,则将其形态与壁虎和Tarentola的骨皮进行表征和比较,以及鳞片内骨皮的多样性。骨皮病被证实存在于斑点壁虎中;然而,这些骨皮是复合的、叠瓦状的鳞状元素,在形态上与闪烁龙和格氏龙中观察到的真皮骨化相似,而壁虎和Tarentola拥有板状和颗粒状的骨皮。我们的研究结果表明,骨皮肤在Gekkota内至少独立进化了三次,表皮解剖结构可能比祖先历史更好地预测骨皮肤形态。需要进一步的研究来研究Geckolepis骨皮肤在皮肤自切后的再生能力。
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引用次数: 17
A new species of Zygaspis (Reptilia: Squamata: Amphisbaenidae) from north-eastern Mozambique 文章标题莫桑比克东北部棘棘蛛属一新种(爬行纲:鳞目:两栖科)
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2016-07-02 DOI: 10.1080/21564574.2016.1183717
D. Broadley, G. Measey
Abstract We describe a new species of Zygaspis based on a single specimen collected in north-eastern Mozambique and deposited in the Natural History Museum of Zimbabwe, Bulawayo. The new species is characterised by its extensively black pigmentation, 191 body annuli, discrete preoculars, four parietals, two postoculars, three supralabials, and two post-supralabials. Phylogenetic analysis of mtDNA suggests that it is a sister taxon to the widespread Zygaspis quadrifrons. ZooBank.—urn:lsid:zoobank.org:pub:9651A65E-949E-4C95-A363-81C558A222AC
摘要基于采集于莫桑比克东北部并保存于津巴布韦布拉瓦约自然历史博物馆的单一标本,我们描述了一种新的Zygaspis。新种的特征是其广泛的黑色色素沉着,191个体环,离散的眼前,4个眼壁,2个眼后,3个眼上和2个眼上。mtDNA的系统发育分析表明,它是广泛分布的四叉叉棘猴(Zygaspis quadrifrons)的姐妹分类群。ZooBank.-urn: lsid zoobank.org:酒吧:9651 a65e - 949 e - 4 - c95 a363 - 81 c558a222ac
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引用次数: 9
Appendicular skeleton of the vestigial-limbed African skink Eumecia anchietae 非洲残肢小蜥蜴的附肢骨架
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2016-07-02 DOI: 10.1080/21564574.2016.1258012
S. Stanley, R. M. Liniewski, P. Senter
Abstract Within the lizard family Scincidae (the skinks) are numerous examples of species with vestigial limbs and girdles, but these vestigial structures remain undescribed for many such species. Here we seek to partially fill this gap in knowledge by describing the vestigial limb and girdle skeletons of Eumecia anchietae, an African skink for which the appendicular skeleton has not previously been described. Radiographs of 10 adult specimens reveal variation in the degree of ossification of the interclavicle and scapula and in the forelimb epiphyses; lengths of the humerus, forelimb and femur; and number of metacarpals, metatarsals and phalanges. The variation is unrelated to specimen size for most of these parameters. In all specimens the sternum is absent, the clavicle is ossified, ossification is greater in the humerus than in the more distal forelimb elements, ossification is greater in the tibia than the fibula, and the third toe has two phalanges. The results of this study suggest caution in the use of details of the morphology of vestigial limbs to diagnose lizard species, because of the variability present in such limbs.
在蜥蜴科(石龙子)中,有许多物种具有退化的四肢和腰带,但这些退化的结构在许多这类物种中仍未被描述。在这里,我们试图通过描述一种非洲石龙子的残余肢体和腰带骨骼来部分填补这一知识空白,这种石龙子的附属骨以前没有被描述过。10例成人标本的x线片显示锁骨间、肩胛骨和前肢骨骺骨化程度的变化;肱骨、前肢和股骨的长度;还有掌骨,跖骨和指骨的数量。大多数这些参数的变化与试样尺寸无关。所有标本胸骨均缺失,锁骨骨化,肱骨骨化程度大于前肢远端,胫骨骨化程度大于腓骨,第三趾有两个指骨。这项研究的结果表明,在使用退化肢体的形态学细节来诊断蜥蜴物种时要谨慎,因为这些肢体存在可变性。
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引用次数: 2
Analysis of genetic diversity in Rose’s mountain toadlet (Capensibufo rosei) using novel microsatellite markers 利用新型微卫星标记分析玫瑰山蟾蜍的遗传多样性
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2016-07-02 DOI: 10.1080/21564574.2016.1234511
J. D. da Silva, K. Feldheim, R. Daniels, S. Edwards, K. Tolley
Abstract On the Cape Peninsula, Capensibufo rosei is known from only two isolated breeding populations within Table Mountain National Park. Because of its declining state, there is an urgent need to understand the genetic diversity, population structure and patterns of movement of this species. To do this, 15 microsatellite primer pairs were designed, optimised and tested. Successful loci were screened for null alleles and genotyping errors and then analysed, specifically noting the number of alleles, allelic size range, observed and expected heterozygosities, deviations from Hardy–Weinberg equilibrium (HWE) and linkage disequilibria. Bottleneck tests and analyses of molecular variance (AMOVA) were also conducted to gain insight into each population’s structure. Eleven primer pairs produced unambiguous polymerase chain reactions (PCR) products and scoreable bands, which were found to be polymorphic across both breeding populations. Deviations from HWE were detected owing to the presence of null alleles and inbreeding. Significant bottleneck signatures were detected for both populations and the AMOVA revealed significant differentiation between the two populations, indicating genetic structure at the population level.
在开普半岛,桌山国家公园内只有两个孤立的繁殖种群。由于其濒危状态,迫切需要了解其遗传多样性、种群结构和迁徙模式。为此,对15对微卫星引物进行了设计、优化和测试。筛选成功基因座的零等位基因和基因分型错误,然后进行分析,特别注意等位基因的数量、等位基因大小范围、观察到的和预期的杂合度、偏离Hardy-Weinberg平衡(HWE)和连锁不平衡。瓶颈测试和分子方差分析(AMOVA)也进行了深入了解每个群体的结构。11对引物产生了明确的聚合酶链反应(PCR)产物和可评分的条带,这些条带在两个育种群体中都是多态性的。由于存在零等位基因和近交,检测到与HWE的偏差。在两个种群中均检测到显著的瓶颈特征,AMOVA显示两个种群之间存在显著的分化,表明在种群水平上存在遗传结构。
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引用次数: 5
Variation in body size and morphometric traits of males and females of the wall gecko, Tarentola delalandii (Phyllodactylidae) from different environments on Tenerife 特内里费岛不同环境壁壁虎体型及形态特征的差异
IF 1.5 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2016-07-02 DOI: 10.1080/21564574.2016.1234512
María de Fuentes-Fernández, María Mercedes Suárez-Rancel, M. Molina-Borja
Abstract We analysed sexual dimorphism in body size and several other morphological traits of two populations of Tarentola delalandii on the island of Tenerife (San Cristóbal de La Laguna in the North, and Granadilla de Abona in the South), whose habitats differ in climate and vegetation cover. Sexual size dimorphism was invariant between populations. Males had significantly larger body size (snout−vent length, SVL) than females and SVL was larger in the northern population than in the southern population. Applying a Permutational MANCOVA to the other morphological variables, considering separately head and trunk lengths as covariates, we found a significant effect of population and gender. For the majority of morphological variables (body mass, SVL, width, length and height of the head, trunk length, forelimb and hindlimb, height and width of the tail) studied (eight in each analysis), there were significant differences, males having larger values than females and northern individuals larger values than those of the southern population. Therefore, we confirmed quantitatively a significant male biased sexual dimorphism in body size in the two populations and have shown sexual dimorphism in the characteristics mentioned previously. However, most of the shape-adjusted traits were significantly larger in females than in males. Results are discussed of possible evolutionary, ontogenetic and ecological factors affecting the expression of sexual dimorphism in the species studied.
摘要本文分析了特内里费岛(Tenerife island)两个不同气候和植被生境的Tarentola delalandii种群(北部的San Cristóbal de La Laguna和南部的Granadilla de Abona)在体型和其他形态特征上的性别二态性。两性大小二态性在种群间是不变的。雄鼠的体长(鼻口长度)显著大于雌鼠,且北方种群的鼻口长度大于南方种群。对其他形态变量应用置换方差分析,分别考虑头长和干长作为协变量,我们发现种群和性别的影响显著。研究的大多数形态学变量(体重、SVL、头部宽度、长高、躯干长度、前肢和后肢、尾巴高度和宽度)(每次分析8个)存在显著差异,雄性个体的值大于雌性,北方个体的值大于南方种群的值。因此,我们在数量上证实了两个种群在体型上存在显著的雄性偏向性二态性,并且在前面提到的特征上也显示了性别二态性。然而,大多数形状调节特征在女性中明显大于男性。结果讨论了可能的进化,个体发生和生态因素影响两性异形的表达在研究的物种。
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引用次数: 4
期刊
African Journal of Herpetology
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