{"title":"Political Institutions, Processes, and Actors","authors":"Daniel C. Levy, G. Székely","doi":"10.4324/9780429036460-3","DOIUrl":"https://doi.org/10.4324/9780429036460-3","url":null,"abstract":"","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"136 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2019-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80143488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Politics of Stability","authors":"Daniel C. Levy, G. Székely","doi":"10.4324/9780429036460-4","DOIUrl":"https://doi.org/10.4324/9780429036460-4","url":null,"abstract":"","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"66 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2019-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83916232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Foreign Policy: In the Giant's Shadow","authors":"Daniel C. Levy, G. Székely","doi":"10.4324/9780429036460-6","DOIUrl":"https://doi.org/10.4324/9780429036460-6","url":null,"abstract":"","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"38 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2019-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73704247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Brief Political History","authors":"Daniel C. Levy, G. Székely","doi":"10.4324/9780429036460-2","DOIUrl":"https://doi.org/10.4324/9780429036460-2","url":null,"abstract":"","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"46 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2019-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76585701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Guégan, G. Suzán, S. Kati-coulibaly, Didier Nkoko Bonpamgue, J. Moatti
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Guegan J-F, Suzan G, Kati-Coulibaly S, Bonpamgue DN, Moatti J-P. Sustainable Development Goal #3, “health and well-being”, and the need for more integrative thinking. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/vmoa.5.2.443 . Recently, the United-Nations adopted 17 sustainable development goals for the 2030 Agenda. The Sustainable Development Goal (SDG) 3 “Ensuring a healthy life and promoting well-being for all ages” is one of the most transversal goals, which is interconnected with the other SDGs. The health and well-being are the aim of this goal and also, they are the result of other goals that empower people to develop better in different social, economic and productive areas. The SDG 3 is a multiple and universal resource on which sustainable development policies can be based, in particular for the most needed countries, and can lead to the sustainable maintenance of well-being and health. However, SDG 3 faces a high sectorization, so there is a risk of not being able to achieve the stated objectives. Only a national and international reflection on human population and animal health surveillance devices, environmental health, implementation of appropriate indicators and specific research funding will ensure the balance between the legitimacy of society’s demands and the needs of scientific and medical excellence. The health and well-being indicators that are needed to achieve the agenda goals are based on reliable and relevant quantitative data, which are currently rare or even non-existent in some regions. Therefore, it is now necessary to initiate a more integrative international animal and public health and research strategy in order to collect new data, particularly those relating to current emerging infectious diseases that affect public and animal health, especially in developing countries. Figure 1. (a) Simplified representation of the relationships between the environment and its different ecological and biogeographic components, depending on the distribution and abundance of infectious diseases and their hosts (vectors and/or reservoirs), and to the individual and family income. The form and severity of infections interact with the income by introducing a complex dynamic between these two parameters.
{"title":"Sustainable Development Goal #3, “health and well-being”, and the need for more integrative thinking","authors":"J. Guégan, G. Suzán, S. Kati-coulibaly, Didier Nkoko Bonpamgue, J. Moatti","doi":"10.21753/VMOA.5.2.443","DOIUrl":"https://doi.org/10.21753/VMOA.5.2.443","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Guegan J-F, Suzan G, Kati-Coulibaly S, Bonpamgue DN, Moatti J-P. Sustainable Development Goal #3, “health and well-being”, and the need for more integrative thinking. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/vmoa.5.2.443 . Recently, the United-Nations adopted 17 sustainable development goals for the 2030 Agenda. The Sustainable Development Goal (SDG) 3 “Ensuring a healthy life and promoting well-being for all ages” is one of the most transversal goals, which is interconnected with the other SDGs. The health and well-being are the aim of this goal and also, they are the result of other goals that empower people to develop better in different social, economic and productive areas. The SDG 3 is a multiple and universal resource on which sustainable development policies can be based, in particular for the most needed countries, and can lead to the sustainable maintenance of well-being and health. However, SDG 3 faces a high sectorization, so there is a risk of not being able to achieve the stated objectives. Only a national and international reflection on human population and animal health surveillance devices, environmental health, implementation of appropriate indicators and specific research funding will ensure the balance between the legitimacy of society’s demands and the needs of scientific and medical excellence. The health and well-being indicators that are needed to achieve the agenda goals are based on reliable and relevant quantitative data, which are currently rare or even non-existent in some regions. Therefore, it is now necessary to initiate a more integrative international animal and public health and research strategy in order to collect new data, particularly those relating to current emerging infectious diseases that affect public and animal health, especially in developing countries. Figure 1. (a) Simplified representation of the relationships between the environment and its different ecological and biogeographic components, depending on the distribution and abundance of infectious diseases and their hosts (vectors and/or reservoirs), and to the individual and family income. The form and severity of infections interact with the income by introducing a complex dynamic between these two parameters.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"5 1","pages":"0-0"},"PeriodicalIF":0.2,"publicationDate":"2018-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.5.2.443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45889715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agustín Nieto-Carmona, Jeny Aguilar-Acevedo, M. S. Rubio-Lozano, Pedro Antonio Alvarado-García, G. Tapia, L. Ocampo-Camberos, H. Sumano
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Nieto-Carmona A, Aguilar-Acevedo J, Rubio M de la S, Alvarado-Garcia P, Tapia G, Ocampo L, Sumano H. Non-inferiority trial of two commercial zilpaterol HCl brands in Bos indicus cattle under humid tropical conditions. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/vmoa.5.2.482 . Commercial availability of a generic zilpaterol HCl (ZH) premix preparation for beef cattle in Mexico motivated a non-inferiority trial vs the reference preparation. The trial was conducted on zebu-type cattle ( Bos indicus ) under humid tropical conditions. Meat production and basic meat quality were assessed for 810 zebu bulls, aged 18-22 months and weighing 430 to 490 kg. Bulls were randomly assigned into one of three groups: ZHg, treated with the generic ZH (Zipamix®) preparation; ZHr, treated with the reference ZH (Zilmax®) preparation, and Cg, the untreated control group. Housing, shade surface, feeding and water availability were highly homogeneous between the animals’ pens. Results for the measured productive and meat quality parameters showed that both ZH-treated groups had higher values than the Cg (P 0.05). Figure 2. Sensorial hedonic scale percentages for aroma, flavour, tenderness and juiciness per treatment as follows: ZHg = zilpaterol hydrochloride from Zipamix® (Pisa Agropecuaria Mexico, Guadalajara, Mexico); ZHr = zilpaterol hydrochloride from Zilmax (MSD).
引用本文:Nieto-Carmona A, Aguilar-Acevedo J, Rubio M de la S, Alvarado-Garcia P, Tapia G, Ocampo L, Sumano H.两种商业品牌的zilpaterol HCl在潮湿热带条件下对籼牛的非效性试验。墨西哥兽医局。2018; 5(2)。Doi: 10.21753/vmoa.5.2.482。一种用于肉牛的齐尔帕特尔盐酸(ZH)预混制剂在墨西哥的商业可用性激发了与参考制剂的非劣效性试验。试验是在潮湿的热带条件下对zebu型牛(Bos indicus)进行的。对810头18-22月龄、体重430 ~ 490 kg的瘤牛进行了肉产和基本肉质评价。公牛被随机分为三组:ZHg组，使用通用的ZH (Zipamix®)制剂;ZHr为对照ZH (Zilmax®)制剂，Cg为未处理对照组。围栏之间的住房、遮荫面、饲料和水的可用性高度均匀。生产性能和肉品质的测定结果显示，zhh处理组的生产性能和肉品质的测定值均高于Cg (p0.05)。图2。感官享乐量表的香气、风味、嫩度和多汁性百分比如下:ZHg =盐酸zilpaterol来自Zipamix®(Pisa Agropecuaria Mexico, Guadalajara, Mexico);zr = Zilmax (MSD)盐酸zilpaterol。
{"title":"Non-inferiority trial of two commercial zilpaterol HCl brands in Bos indicus cattle under humid tropical conditions","authors":"Agustín Nieto-Carmona, Jeny Aguilar-Acevedo, M. S. Rubio-Lozano, Pedro Antonio Alvarado-García, G. Tapia, L. Ocampo-Camberos, H. Sumano","doi":"10.21753/VMOA.5.2.482","DOIUrl":"https://doi.org/10.21753/VMOA.5.2.482","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Nieto-Carmona A, Aguilar-Acevedo J, Rubio M de la S, Alvarado-Garcia P, Tapia G, Ocampo L, Sumano H. Non-inferiority trial of two commercial zilpaterol HCl brands in Bos indicus cattle under humid tropical conditions. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/vmoa.5.2.482 . Commercial availability of a generic zilpaterol HCl (ZH) premix preparation for beef cattle in Mexico motivated a non-inferiority trial vs the reference preparation. The trial was conducted on zebu-type cattle ( Bos indicus ) under humid tropical conditions. Meat production and basic meat quality were assessed for 810 zebu bulls, aged 18-22 months and weighing 430 to 490 kg. Bulls were randomly assigned into one of three groups: ZHg, treated with the generic ZH (Zipamix®) preparation; ZHr, treated with the reference ZH (Zilmax®) preparation, and Cg, the untreated control group. Housing, shade surface, feeding and water availability were highly homogeneous between the animals’ pens. Results for the measured productive and meat quality parameters showed that both ZH-treated groups had higher values than the Cg (P 0.05). Figure 2. Sensorial hedonic scale percentages for aroma, flavour, tenderness and juiciness per treatment as follows: ZHg = zilpaterol hydrochloride from Zipamix® (Pisa Agropecuaria Mexico, Guadalajara, Mexico); ZHr = zilpaterol hydrochloride from Zilmax (MSD).","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":" ","pages":"0-0"},"PeriodicalIF":0.2,"publicationDate":"2018-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.5.2.482","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48622139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. J. Barrón-Rodríguez, F. Chávez-Maya, E. Loza-Rubio, G. García-Espinosa
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Barron-Rodriguez RJ, Chavez-Maya F, Loza-Rubio E, Garcia-Espinosa G. Isolation and characterization of influenza A virus (H6N2) from a temporary artificial pond in Mexico. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/5.2.475. . Most epidemiological surveillance studies of the influenza A virus (IAV) have focused on the isolation and detection of the virus in wild birds. However, there are limited descriptions of both the wild duck population and the purpose and size of the aquatic habitats where viruses have been detected or isolated. The objective of this study was to determine if a pond of 16 hectares (39.536 acres), used for agricultural and fishing purposes and visited by approximately 9000 wild migratory ducks consisting of nine different species during the wintering stay, is suitable to support the isolation of IAV. One influenza A virus was isolated from Pekin ducks used as sentinels during the wintering stay season from September 2007 to March 2008. Only one IAV subtype was isolated from 9 of the 88 samples collected from the sentinel ducks over seven months, and the molecular characterization of this isolate revealed an H6N2 virus subtype. Based on this information, it is suggested that a pond such as the one in this study provides a suitable biological setting to support the presence of IAV, but the minimum biological environment to isolate the influenza A virus is still unknown. Figure 1. Location of the artificial pond in the State of Mexico with 19°24’22” N, 99°41’36” W coordinates and the routes of migratory birds in America (Google Earth).
{"title":"Isolation and characterization of influenza A virus (H6N2) from a temporary artificial pond in Mexico","authors":"R. J. Barrón-Rodríguez, F. Chávez-Maya, E. Loza-Rubio, G. García-Espinosa","doi":"10.21753/VMOA.5.2.475","DOIUrl":"https://doi.org/10.21753/VMOA.5.2.475","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Barron-Rodriguez RJ, Chavez-Maya F, Loza-Rubio E, Garcia-Espinosa G. Isolation and characterization of influenza A virus (H6N2) from a temporary artificial pond in Mexico. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/5.2.475. . Most epidemiological surveillance studies of the influenza A virus (IAV) have focused on the isolation and detection of the virus in wild birds. However, there are limited descriptions of both the wild duck population and the purpose and size of the aquatic habitats where viruses have been detected or isolated. The objective of this study was to determine if a pond of 16 hectares (39.536 acres), used for agricultural and fishing purposes and visited by approximately 9000 wild migratory ducks consisting of nine different species during the wintering stay, is suitable to support the isolation of IAV. One influenza A virus was isolated from Pekin ducks used as sentinels during the wintering stay season from September 2007 to March 2008. Only one IAV subtype was isolated from 9 of the 88 samples collected from the sentinel ducks over seven months, and the molecular characterization of this isolate revealed an H6N2 virus subtype. Based on this information, it is suggested that a pond such as the one in this study provides a suitable biological setting to support the presence of IAV, but the minimum biological environment to isolate the influenza A virus is still unknown. Figure 1. Location of the artificial pond in the State of Mexico with 19°24’22” N, 99°41’36” W coordinates and the routes of migratory birds in America (Google Earth).","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":" ","pages":"0-0"},"PeriodicalIF":0.2,"publicationDate":"2018-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.5.2.475","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49161237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Félix Leao Rodríguez Fierros, Gerardo F. Quiroz Rocha, L. N. Ochoa, A. A. Cecílio, E. Hernández
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Rodriguez Fierros FL, Quiroz Rocha G, Nunez Ochoa L, Aparicio Cecilio A, Salgado Hernandez EG. Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.386 . Hepatic lipidosis is a metabolic disorder that occurs frequently in dairy cattle. Its clinical evaluation on dairy farms is complex, as it is based on physical, chemical and biological tests. The present study evaluates the practical use of hepatic cytology in dairy cattle and suggests that overall health and dairy production rates of animals are not compromised with this procedure. Cell and blood samples were obtained from 25 randomly selected multiparous Holstein dairy cows at three different times during the transition period (at calving and at 10 and 21 days postpartum). The values of haematocrit, plasma protein, fibrinogen, and total and differential leucocyte counts were determined, together with total protein, albumin, glutamate dehydrogenase, triacylglycerides, cholesterol and s-hydroxybutyrate. Cytological results were classified by the detected degree of fatty infiltration, and haematological and metabolic results were compared between each sampled period. The health status and the daily milk production at each sampling stage were recorded. Obtained results indicated that the animals did not show significant clinical inflammation or a decrease in dairy production, which could be deemed secondary to the cytological sampling. This technique is thus regarded as a feasible alternative for the diagnosis of hepatic lipidosis in dairy cattle. Figure 1. Cell aspiration from cow liver in 11th right intercostal space approximately 15 to 20 cm below the spinous apophysis.
{"title":"Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle","authors":"Félix Leao Rodríguez Fierros, Gerardo F. Quiroz Rocha, L. N. Ochoa, A. A. Cecílio, E. Hernández","doi":"10.21753/VMOA.5.1.386","DOIUrl":"https://doi.org/10.21753/VMOA.5.1.386","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Rodriguez Fierros FL, Quiroz Rocha G, Nunez Ochoa L, Aparicio Cecilio A, Salgado Hernandez EG. Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.386 . Hepatic lipidosis is a metabolic disorder that occurs frequently in dairy cattle. Its clinical evaluation on dairy farms is complex, as it is based on physical, chemical and biological tests. The present study evaluates the practical use of hepatic cytology in dairy cattle and suggests that overall health and dairy production rates of animals are not compromised with this procedure. Cell and blood samples were obtained from 25 randomly selected multiparous Holstein dairy cows at three different times during the transition period (at calving and at 10 and 21 days postpartum). The values of haematocrit, plasma protein, fibrinogen, and total and differential leucocyte counts were determined, together with total protein, albumin, glutamate dehydrogenase, triacylglycerides, cholesterol and s-hydroxybutyrate. Cytological results were classified by the detected degree of fatty infiltration, and haematological and metabolic results were compared between each sampled period. The health status and the daily milk production at each sampling stage were recorded. Obtained results indicated that the animals did not show significant clinical inflammation or a decrease in dairy production, which could be deemed secondary to the cytological sampling. This technique is thus regarded as a feasible alternative for the diagnosis of hepatic lipidosis in dairy cattle. Figure 1. Cell aspiration from cow liver in 11th right intercostal space approximately 15 to 20 cm below the spinous apophysis.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"5 1","pages":"1-14"},"PeriodicalIF":0.2,"publicationDate":"2018-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.5.1.386","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49378390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. García-Lara, A. Morales-Martínez, Quetzalli D Angeles-López, Hilda Pedraza-Espitia, I. Pérez-Neri, C. Rodríguez-Balderas, F. Pérez-Severiano
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Garcia-Lara L, Morales-Martinez A, Angeles-Lopez QD, Pedraza-Espitia H, Perez-Neri I, Rodriguez-Balderas CA, Perez-Severiano F. Establishment and maintenance of an R6/1 transgenic mouse colony and validation of its progressive neurological phenotype to study Huntington’s disease. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.487 . Huntington’s disease (HD) is a hereditary neurodegenerative disorder of the central nervous system that mainly affects the basal ganglia and has no cure. The mutation is located at an abnormal expansion of the CAG triplet in the Huntingtin gene. Humans show psychiatric, behavioural and motor disorders. Transgenic animal models are essential to the study of HD since the disease only affects humans. Therefore, the aim of this article was to describe the formation and maintenance of and to validate the progressive neurological phenotype of an R6/1 transgenic mouse colony. To achieve our objective, the colony founder was imported from Jackson Laboratories, and the mice were kept under controlled environmental conditions. The animals were bred at the vivarium of the Instituto Nacional de Neurologia y Neurocirugia Manuel Velasco Suarez. The R6/1 transgenic mice were successfully bred and showed genetic and phenotypic characteristics similar to the ones previously reported. Our colony is currently established and validated with the conditions of our vivarium and has produced more than four generations of R6/1 mice. The establishment of the R6/1 colony and its maintenance through generation is an advantage since it allows us to follow the authenticity of the transgenic mice regarding their phenotypic and motor behaviours. Furthermore, these animals can be compared with other transgenic mice that reproduce some of the main characteristics of the disease manifested in humans, making these transgenic R6/1 mice a useful tool for the study of HD. Figure 2. D) Wt mouse with normal fur and weight.
{"title":"Establishment and maintenance of an R6/1 transgenic mouse colony and validation of its progressive neurological phenotype to study Huntington’s disease","authors":"L. García-Lara, A. Morales-Martínez, Quetzalli D Angeles-López, Hilda Pedraza-Espitia, I. Pérez-Neri, C. Rodríguez-Balderas, F. Pérez-Severiano","doi":"10.21753/VMOA.5.1.487","DOIUrl":"https://doi.org/10.21753/VMOA.5.1.487","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Garcia-Lara L, Morales-Martinez A, Angeles-Lopez QD, Pedraza-Espitia H, Perez-Neri I, Rodriguez-Balderas CA, Perez-Severiano F. Establishment and maintenance of an R6/1 transgenic mouse colony and validation of its progressive neurological phenotype to study Huntington’s disease. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.487 . Huntington’s disease (HD) is a hereditary neurodegenerative disorder of the central nervous system that mainly affects the basal ganglia and has no cure. The mutation is located at an abnormal expansion of the CAG triplet in the Huntingtin gene. Humans show psychiatric, behavioural and motor disorders. Transgenic animal models are essential to the study of HD since the disease only affects humans. Therefore, the aim of this article was to describe the formation and maintenance of and to validate the progressive neurological phenotype of an R6/1 transgenic mouse colony. To achieve our objective, the colony founder was imported from Jackson Laboratories, and the mice were kept under controlled environmental conditions. The animals were bred at the vivarium of the Instituto Nacional de Neurologia y Neurocirugia Manuel Velasco Suarez. The R6/1 transgenic mice were successfully bred and showed genetic and phenotypic characteristics similar to the ones previously reported. Our colony is currently established and validated with the conditions of our vivarium and has produced more than four generations of R6/1 mice. The establishment of the R6/1 colony and its maintenance through generation is an advantage since it allows us to follow the authenticity of the transgenic mice regarding their phenotypic and motor behaviours. Furthermore, these animals can be compared with other transgenic mice that reproduce some of the main characteristics of the disease manifested in humans, making these transgenic R6/1 mice a useful tool for the study of HD. Figure 2. D) Wt mouse with normal fur and weight.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"5 1","pages":"22-33"},"PeriodicalIF":0.2,"publicationDate":"2018-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.5.1.487","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48649220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. R. B. Garfias, T. Rodríguez, C. Rojas, J. Lira, J. A. Álvarez, Diego Polanco
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Bautista-Garfias CR, Rodriguez T, Rojas C, Lira JJ, Alvarez JA, Polanco D. Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.436 . Data on the implication of the stable fly ( Stomoxys calcitrans , Diptera: Muscidae), in the transmission of Anaplasma marginale in tick-free cattle is scarce. Hence, the objective of this investigation was to detect the presence of DNA from A. marginale in stable flies caught near a bovine herd, which has been maintained free of ticks for 40 years, and occasionally presents some clinical cases of anaplasmosis. Twenty-four batches of S. calcitrans (15 flies each) were collected in the morning and in the afternoon twice a week (except for one catch in one week of September and one catch in one week of December) during 12 catching days in a period of four months. Their DNA was obtained and analysed by nested PCR (nPCR) to identify the presence of A. marginale DNA. Seven of the batches (29.16 %) were positive for A. marginale , as detected by an nPCR that targets the A. marginale msp5 gene. Similarly, in two out of 12 catching days (16.66 %), those S. calcitrans batches collected in the morning and in the afternoon, were positive for A. marginale , while those S. calcitrans groups collected in three out of 12 catching days (25 %), were positive for the rickettsia. The obtained results suggest that A. marginale transmission is carried out mechanically by S. calcitrans , favouring the circulation and maintenance of the microorganism in this particular bovine herd. Figure 1. A. Example of a stable fly Stomoxys calcitrans caught in this study (photograph: Carlos R. Bautista).
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