Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Losada-Espinosa N,Trujillo Ortega ME, Galindo Maldonado FA. The welfare of pigs in rustic and technified production systems using the Welfare Quality protocols of pigs in Mexico: Validity of indicators of animal welfare as part of the sustainability criteria of pig production systems. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.521 . The Welfare Quality® (WQ) protocols have been developed as a tool for the assessment of farm animal welfare based on scientific evidence. Animal welfare (AW) is part of the sustainability criteria of livestock production. A study was carried out in four states of Central Mexico in seven rustic (rPS) and six technified (tPS) production systems using the WQ protocol with the objective of providing an initial approximation of the welfare of animals and to discuss the validity of indicators of AW. The results showed that the animals housed in rustic units presented better results in the Good Health category and with respect to the criterion expression of social behaviour, while the frequency of criteria concerning Positive emotional states was higher in animals in the technified units. In the changing context in which the farms operate, including changing agricultural policies, new environmental and food safety regulations, variability of climatic conditions, and volatility in prices of inputs and outputs, it is not only the attributes referring to productivity and efficiency that become relevant. It is concluded that the criteria related to the WQ principles of health and behaviour are sensitive to changes in the housing and management of pigs. The high occurrences of health and behaviour problems recorded in technified systems are an indicator of poor welfare. Figure 4 . Classification of rustic (RS) and technified (TS) production units within the welfare categories proposed by the Welfare Quality protocol.
Veterinaria Mexico OA ISSN:2448-6760引用如下：Losada Espinosa N，Trujillo Ortega ME，Galindo Maldonado FA。使用墨西哥猪福利质量协议的乡村和技术化生产系统中的猪福利：作为猪生产系统可持续性标准一部分的动物福利指标的有效性。兽医墨西哥OA。2017年；4（4）。doi:10.21753/vmoa.4.4.521.福利质量®（WQ）协议已被开发为基于科学证据评估农场动物福利的工具。动物福利（AW）是畜牧业生产可持续性标准的一部分。使用WQ协议在墨西哥中部的四个州对七个乡村（rPS）和六个技术化（tPS）生产系统进行了一项研究，目的是提供动物福利的初步近似值，并讨论AW指标的有效性。结果表明，在良好健康类别和社会行为的标准表达方面，居住在乡村单元的动物表现出更好的结果，而在技术单元的动物中，关于积极情绪状态的标准出现的频率更高。在农场经营的不断变化的背景下，包括不断变化的农业政策、新的环境和食品安全法规、气候条件的可变性以及投入和产出价格的波动性，相关的不仅仅是生产力和效率的属性。得出的结论是，与WQ健康和行为原则相关的标准对猪的饲养和管理变化很敏感。技术化系统中记录的健康和行为问题的高发生率是福利不佳的一个指标。图4。福利质量协议提出的福利类别中的乡村（RS）和技术化（TS）生产单位的分类。
{"title":"The welfare of pigs in rustic and technified production systems using the Welfare Quality protocols of pigs in Mexico: Validity of indicators of animal welfare as part of the sustainability criteria of pig production systems","authors":"N. Losada-Espinosa, M. T. Ortega, F. Galindo","doi":"10.21753/VMOA.4.4.521","DOIUrl":"https://doi.org/10.21753/VMOA.4.4.521","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Losada-Espinosa N,Trujillo Ortega ME, Galindo Maldonado FA. The welfare of pigs in rustic and technified production systems using the Welfare Quality protocols of pigs in Mexico: Validity of indicators of animal welfare as part of the sustainability criteria of pig production systems. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.521 . The Welfare Quality® (WQ) protocols have been developed as a tool for the assessment of farm animal welfare based on scientific evidence. Animal welfare (AW) is part of the sustainability criteria of livestock production. A study was carried out in four states of Central Mexico in seven rustic (rPS) and six technified (tPS) production systems using the WQ protocol with the objective of providing an initial approximation of the welfare of animals and to discuss the validity of indicators of AW. The results showed that the animals housed in rustic units presented better results in the Good Health category and with respect to the criterion expression of social behaviour, while the frequency of criteria concerning Positive emotional states was higher in animals in the technified units. In the changing context in which the farms operate, including changing agricultural policies, new environmental and food safety regulations, variability of climatic conditions, and volatility in prices of inputs and outputs, it is not only the attributes referring to productivity and efficiency that become relevant. It is concluded that the criteria related to the WQ principles of health and behaviour are sensitive to changes in the housing and management of pigs. The high occurrences of health and behaviour problems recorded in technified systems are an indicator of poor welfare. Figure 4 . Classification of rustic (RS) and technified (TS) production units within the welfare categories proposed by the Welfare Quality protocol.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":" ","pages":"0-0"},"PeriodicalIF":0.2,"publicationDate":"2017-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.4.521","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47240776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. M. Ramírez, R. Ponce, C. Ruíz, Graciela Castañeda Aceves, Ernesto Marín Flamand, G. Fariña, H. Álvarez
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Turral Ramirez MM, Cordova Ponce R, Gonzalez Ruiz C, Castaneda Aceves G, Marin Flamand E, Garrido Farina G, Ramirez Alvarez H. First identification of Herpesvirus infections among endemic and exotic psittacines in Mexico. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.469 . The illegal trafficking of exotic birds such as parrots is now the third most lucrative business in the world and has been a problem for several years. As a result of this trafficking, there has been an increase in the emergence of exotic diseases. Herpesvirus is a pathogen of psittacines that has not been identified in Mexico to date. Through the use of polymerase chain reaction (PCR) assays and pathological analyses, the present study demonstrates the presence of herpesvirus in endemic and exotic psittacines in Mexico. The study was based on 50 birds, with clinical signs of herpervirus infection, and 45 clinically healthy birds. DNA samples were obtained from proctodaeal cells of the healthy birds, collected through the use of an enema; additionally, the liver, kidneys, brain, large intestines, and lungs of five birds exhibiting clinical signs suggestive of herpesvirus infection, were collected and fixed in buffered formalin for histopathological examination. PCR assays revealed herpesvirus infection in 8 clinically healthy birds, including four exotic species, and one endemic species. On necropsy, four of the five birds with clinical signs of herpesvirus infection showed lesions caused by herpesvirus, and the presence of intranuclear inclusion bodies in various tissues. Thus, these results demonstrate that there is a high risk of herpesvirus infection in endemic Mexican psittacines, which could significantly impact the health of these populations. Figure 5. a) Liver with mononuclear infiltrate (MNI) (100× magnification). The image shows the parenchyma at the interstitial level with a mononuclear inflammatory infiltrate characterized by the presence of lymphocytes (L), plasma cells (P), and macrophages (MO). b) Liver with necrosis and mononuclear infiltrate (40× magnification). Zones of coagulative necrosis (N) delimited by the inflammatory mononuclear infiltrate (arrow) are evident. c) Kidney with necrosis and mononuclear infiltrate (10× magnification). Renal parenchyma with extensive areas of coagulative necrosis (N) between the cortex and the medulla were observed. These areas were characterized by a marked loss of architecture and cellular detail and were delimited by mononuclear inflammatory cells (arrows). d) Necrotic intestine (100× magnification). The intestine developed severe coagulative necrosis (N) throughout the mucosa. Additionally, inflammatory infiltrates of mononuclear cells were observed near the basal membrane of the mucosa (arrows).
{"title":"First identification of Herpesvirus infections among endemic and exotic psittacines in Mexico","authors":"M. M. Ramírez, R. Ponce, C. Ruíz, Graciela Castañeda Aceves, Ernesto Marín Flamand, G. Fariña, H. Álvarez","doi":"10.21753/VMOA.4.4.469","DOIUrl":"https://doi.org/10.21753/VMOA.4.4.469","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Turral Ramirez MM, Cordova Ponce R, Gonzalez Ruiz C, Castaneda Aceves G, Marin Flamand E, Garrido Farina G, Ramirez Alvarez H. First identification of Herpesvirus infections among endemic and exotic psittacines in Mexico. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.469 . The illegal trafficking of exotic birds such as parrots is now the third most lucrative business in the world and has been a problem for several years. As a result of this trafficking, there has been an increase in the emergence of exotic diseases. Herpesvirus is a pathogen of psittacines that has not been identified in Mexico to date. Through the use of polymerase chain reaction (PCR) assays and pathological analyses, the present study demonstrates the presence of herpesvirus in endemic and exotic psittacines in Mexico. The study was based on 50 birds, with clinical signs of herpervirus infection, and 45 clinically healthy birds. DNA samples were obtained from proctodaeal cells of the healthy birds, collected through the use of an enema; additionally, the liver, kidneys, brain, large intestines, and lungs of five birds exhibiting clinical signs suggestive of herpesvirus infection, were collected and fixed in buffered formalin for histopathological examination. PCR assays revealed herpesvirus infection in 8 clinically healthy birds, including four exotic species, and one endemic species. On necropsy, four of the five birds with clinical signs of herpesvirus infection showed lesions caused by herpesvirus, and the presence of intranuclear inclusion bodies in various tissues. Thus, these results demonstrate that there is a high risk of herpesvirus infection in endemic Mexican psittacines, which could significantly impact the health of these populations. Figure 5. a) Liver with mononuclear infiltrate (MNI) (100× magnification). The image shows the parenchyma at the interstitial level with a mononuclear inflammatory infiltrate characterized by the presence of lymphocytes (L), plasma cells (P), and macrophages (MO). b) Liver with necrosis and mononuclear infiltrate (40× magnification). Zones of coagulative necrosis (N) delimited by the inflammatory mononuclear infiltrate (arrow) are evident. c) Kidney with necrosis and mononuclear infiltrate (10× magnification). Renal parenchyma with extensive areas of coagulative necrosis (N) between the cortex and the medulla were observed. These areas were characterized by a marked loss of architecture and cellular detail and were delimited by mononuclear inflammatory cells (arrows). d) Necrotic intestine (100× magnification). The intestine developed severe coagulative necrosis (N) throughout the mucosa. Additionally, inflammatory infiltrates of mononuclear cells were observed near the basal membrane of the mucosa (arrows).","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":" ","pages":"0-0"},"PeriodicalIF":0.2,"publicationDate":"2017-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.4.469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45759176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Manuel Santana Pérez, M. A. D. L. Varga, Juan García, V. G. Lacasa
Veterinaria Mexico OA ISSN: 2448-6760 Como citar este articulo: Lomillos Perez JM, Alonso de la Varga ME, Garcia JJ, Gaudioso Lacasa VR. Monitorizacion del ganado lidia con tecnologia GPS-GPRS; estudio del comportamiento en pastoreo y la distribucion espacial. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.405 . En los ultimos anos, el comportamiento del ganado vacuno en pastoreo ha sido poco estudiado, en comparacion con el comportamiento en granja. Ademas, en determinadas razas criadas en sistemas extensivos, la presencia humana puede modificar o interrumpir sus pautas etologicas: los animales se alejan de quien los observa. El uso de la tecnologia GPS-GPRS en la monitorizacion de bovinos explotados en regimen extensivo nos permite disponer de datos de interes en tiempo real; proporciona informacion sobre desplazamientos, distancias recorridas, territorio pastoreado, zonas mas frecuentadas, pautas de comportamiento, etc. En esta investigacion, se monitorizaron, mediante la implantacion de collares GPS, 21 vacas de diferentes edades, de tres ganaderias de la provincia de Salamanca pertenecientes a la raza bovina lidia. El estudio se llevo a cabo durante ocho meses, distribuimos a los animales en cercados de diferentes dimensiones y caracteristicas orograficas, y recogimos datos de su posicion geografica cada 15 minutos. Ademas de corroborar el buen funcionamiento de los dispositivos GPS, se establecio el area de campeo para cada animal: la media fue de 56 ha. Elaboramos un grafico de su ritmo circadiano con las distancias recorridas por horas, donde se observa una tendencia a iniciar la actividad diaria unas horas antes del amanecer, atenuar su actividad al anochecer y durante la noche, asi como mantener una fase de reposo nocturno de aproximadamente siete horas. Tambien analizamos la distancia diaria recorrida (3.15 km media/animal), donde encontramos diferencias sustanciales en funcion de la edad, la superficie/animal disponible en el cercado, el dia y la noche, y la epoca del ano. Nuestros resultados son valiosos para un mejor manejo de las superficies pastables con cercados en los que los animales utilicen toda la superficie disponible. Figura 3. Imagen del cercado 5 con las posiciones de los tres animales monitorizados. Rojo, amarillo y azul.
引用本文:Lomillos Perez JM, Alonso de la Varga ME, Garcia JJ, Gaudioso Lacasa VR。利用GPS-GPRS技术监测牲畜;放牧行为和空间分布的研究。兽医墨西哥OA。2017年;4(4)。他的父亲是一名律师，母亲是一名律师。在过去的几年里，与农场的行为相比，人们对放牧牛的行为的研究很少。此外，在广泛饲养的某些品种中，人类的存在可能会改变或破坏它们的行为学模式:动物会远离观察它们的人。使用GPS-GPRS技术监测广泛饲养的牛，使我们能够实时获得感兴趣的数据;提供有关旅行、旅行距离、放牧区域、最常去的地区、行为模式等信息。在本研究中，采用GPS项圈对萨拉曼卡省3个属于lidia牛品种的21头不同年龄的奶牛进行了监测。这项研究进行了8个月，我们将动物分布在不同大小和地形特征的围栏中，每15分钟收集一次它们的地理位置数据。为了确认GPS设备的正常运行，我们为每只动物设置了露营面积:平均为56公顷。制定一个图的节奏与神志迷乱行走的路程时,注意到一个趋势开始每天黎明前几个小时的活动,减少其活动在傍晚和夜间断电,就是保持夜间睡眠阶段约7小时。我们还分析了每天的旅行距离(平均3.15公里/动物)，我们发现年龄、围栏内可用的面积/动物、白天和晚上和一年中的时间有很大的差异。我们的研究结果对于更好地管理可食用区域是有价值的，在围栏中，动物使用所有可用的区域。图3。围栏5的图像与3只动物的位置监测。红色，黄色和蓝色。
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R. Chávez, Ramón Miguel Molina Barrios, J. Chávez, Jorge Robles Mascareño, Jesus Escalante, Martín Acuña Yanes
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Osuna Chavez RF, Molina Barrios RM, Hernandez Chavez JF, Robles Mascareno J, Icedo Escalante JGA, Acuna Yanes M. First report of biovar 6 in birds immunized against Gallibacterium anatis in poultry farms located in Sonora, Mexico. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.389 . Identification of the predominant circulating biovars is important for controlling Gallibacterium anatis infections in poultry because efficient protection is based on the use of biovar-specific immunogens. Twenty-three isolates of Gallibacterium anatis were recovered from commercial laying hens with respiratory and reproductive clinical signs. Bacteria were identified as G. anatis based on morphology, biochemistry, and PCR testing. Biochemical analyses identified the isolates as biovar 6 which had not previously been reported in Sonora, Mexico. Therefore, the recent finding of biovar type 6 could explain clinical presentations of this disease in immunized commercial laying flocks in Sonora. Figure 1. PCR amplification gene, Gallibacterium anatis .
{"title":"First report of biovar 6 in birds immunized against Gallibacterium anatis in poultry farms located in Sonora, Mexico.","authors":"R. Chávez, Ramón Miguel Molina Barrios, J. Chávez, Jorge Robles Mascareño, Jesus Escalante, Martín Acuña Yanes","doi":"10.21753/VMOA.4.3.389","DOIUrl":"https://doi.org/10.21753/VMOA.4.3.389","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Osuna Chavez RF, Molina Barrios RM, Hernandez Chavez JF, Robles Mascareno J, Icedo Escalante JGA, Acuna Yanes M. First report of biovar 6 in birds immunized against Gallibacterium anatis in poultry farms located in Sonora, Mexico. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.389 . Identification of the predominant circulating biovars is important for controlling Gallibacterium anatis infections in poultry because efficient protection is based on the use of biovar-specific immunogens. Twenty-three isolates of Gallibacterium anatis were recovered from commercial laying hens with respiratory and reproductive clinical signs. Bacteria were identified as G. anatis based on morphology, biochemistry, and PCR testing. Biochemical analyses identified the isolates as biovar 6 which had not previously been reported in Sonora, Mexico. Therefore, the recent finding of biovar type 6 could explain clinical presentations of this disease in immunized commercial laying flocks in Sonora. Figure 1. PCR amplification gene, Gallibacterium anatis .","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2017-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.3.389","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44268250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Serrato Lopez AG, Montesinos Montesinos JJ, Anzaldua Arce SR. The endometrium as a source of mesenchymal stem cells in domestic animals and possible applications in veterinary medicine. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.441 . Mesenchymal stem cells (MSCs) have been isolated from the endometrium of humans, mice, cows, pigs and ewes. Typically, these cells are detected in the deep regions of the endometrium, closer to the union with the myometrium. MSCs possess characteristics such as clonogenicity and multipotentiality since they can differentiate in vitro into adipogenic, chondrogenic and osteogenic lineages. These cells can be induced to differentiate in vitro not only into the mesodermal lineage but also into the endodermal and ectodermal lineages. Therefore, MSCs show a great regenerative capacity for various organs and tissues, including the endometrium. Some advantages of endometrial MSCs compared with other MSC sources are their immune modulating activity, their ease of obtainment, and the amount of sample that may be collected. The study of endometrial MSCs in domestic animals is a new and promising field because increasing our understanding of the physiology and biology of these cells may lead to a better understanding of the physiopathology of reproductive diseases, and the development of treatment methods for infertility problems. In other veterinary medicine fields, MSCs can be used for the treatment of autoimmune diseases, cardiac affections, musculoskeletal and articular lesions, muscle degeneration, type 1 diabetes, urinary tract diseases, neurodegenerative processes and tumours. Finally, MSCs are also an important clinical tool for tissue engineering and regenerative medicine. The aim of this review is to present an updated outlook of the knowledge regarding endometrial MSCs and their possible applications in veterinary medicine. Figure 1: Immunoregulatory ability of MSCs. MSCs regulate the functions of NK cells, dendritic cells (DC) and T lymphocytes. The immunosuppressive effect may occur through the secretion of different factors or through cellular contact (black arrows). The former pathway involves TGFs, HGF, IL-10, PGE2, and HLA-G5, whereas the latter pathway involves the products of IDO enzyme activity, PD-L1, HLA-G1, ICAM-I and VCAM-I. Pro-inflammatory cytokines (IFN-?) secreted by NK cells and activated T lymphocytes favour the immunoregulatory activity of MSCs (dotted lines), because they increase or induce the secretion of molecules that regulate the functions of the distinct cellular components of the immune system. Modified from Montesinos et al, and Ma et al. 19,66
Veterinaria Mexico OA ISSN:2448-6760引用如下：Serrato Lopez AG，Montesinos Montesinos JJ，Anzaldua Arce SR。子宫内膜作为家畜间充质干细胞的来源，在兽医学中的可能应用。兽医墨西哥OA。2017年；4（3）。doi:10.21753/vmoa.4.3.441.从人类、小鼠、奶牛、猪和母羊的子宫内膜中分离出间充质干细胞。通常，这些细胞在子宫内膜的深层区域检测到，更靠近与子宫肌层的结合。MSCs具有克隆性和多能性等特征，因为它们可以在体外分化为成脂肪、成软骨和成骨谱系。这些细胞不仅可以在体外诱导分化为中胚层谱系，还可以分化为内胚层和外胚层谱系。因此，间充质干细胞对包括子宫内膜在内的各种器官和组织表现出强大的再生能力。与其他MSC来源相比，子宫内膜MSC的一些优势在于其免疫调节活性、易于获得以及可收集的样本量。在家畜中研究子宫内膜间充质干细胞是一个新的和有前景的领域，因为增加我们对这些细胞的生理学和生物学的了解可能会更好地了解生殖疾病的病理生理学，并开发不孕问题的治疗方法。在其他兽医领域，MSCs可用于治疗自身免疫性疾病、心脏病、肌肉骨骼和关节病变、肌肉变性、1型糖尿病、尿路疾病、神经退行性过程和肿瘤。最后，间充质干细胞也是组织工程和再生医学的重要临床工具。这篇综述的目的是对子宫内膜间充质干细胞及其在兽医学中的可能应用的最新认识进行展望。图1:MSCs的免疫调节能力。MSCs调节NK细胞、树突状细胞（DC）和T淋巴细胞的功能。免疫抑制作用可能通过分泌不同的因子或通过细胞接触发生（黑色箭头）。前者途径涉及TGFs、HGF、IL-10、PGE2和HLA-G5，而后者途径涉及IDO酶活性、PD-L1、HLA-G1、ICAM-I和VCAM-I的产物。NK细胞和活化的T淋巴细胞分泌的促炎细胞因子（IFN-？）有利于MSC的免疫调节活性（虚线），因为它们增加或诱导调节免疫系统不同细胞成分功能的分子的分泌。修改自Montesinos等人和Ma等人19，66
{"title":"The endometrium as a source of mesenchymal stem cells in domestic animals and possible applications in veterinary medicine","authors":"Ana G. Serrato López, J. Montesinos, S. Arce","doi":"10.21753/VMOA.4.3.441","DOIUrl":"https://doi.org/10.21753/VMOA.4.3.441","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Serrato Lopez AG, Montesinos Montesinos JJ, Anzaldua Arce SR. The endometrium as a source of mesenchymal stem cells in domestic animals and possible applications in veterinary medicine. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.441 . Mesenchymal stem cells (MSCs) have been isolated from the endometrium of humans, mice, cows, pigs and ewes. Typically, these cells are detected in the deep regions of the endometrium, closer to the union with the myometrium. MSCs possess characteristics such as clonogenicity and multipotentiality since they can differentiate in vitro into adipogenic, chondrogenic and osteogenic lineages. These cells can be induced to differentiate in vitro not only into the mesodermal lineage but also into the endodermal and ectodermal lineages. Therefore, MSCs show a great regenerative capacity for various organs and tissues, including the endometrium. Some advantages of endometrial MSCs compared with other MSC sources are their immune modulating activity, their ease of obtainment, and the amount of sample that may be collected. The study of endometrial MSCs in domestic animals is a new and promising field because increasing our understanding of the physiology and biology of these cells may lead to a better understanding of the physiopathology of reproductive diseases, and the development of treatment methods for infertility problems. In other veterinary medicine fields, MSCs can be used for the treatment of autoimmune diseases, cardiac affections, musculoskeletal and articular lesions, muscle degeneration, type 1 diabetes, urinary tract diseases, neurodegenerative processes and tumours. Finally, MSCs are also an important clinical tool for tissue engineering and regenerative medicine. The aim of this review is to present an updated outlook of the knowledge regarding endometrial MSCs and their possible applications in veterinary medicine. Figure 1: Immunoregulatory ability of MSCs. MSCs regulate the functions of NK cells, dendritic cells (DC) and T lymphocytes. The immunosuppressive effect may occur through the secretion of different factors or through cellular contact (black arrows). The former pathway involves TGFs, HGF, IL-10, PGE2, and HLA-G5, whereas the latter pathway involves the products of IDO enzyme activity, PD-L1, HLA-G1, ICAM-I and VCAM-I. Pro-inflammatory cytokines (IFN-?) secreted by NK cells and activated T lymphocytes favour the immunoregulatory activity of MSCs (dotted lines), because they increase or induce the secretion of molecules that regulate the functions of the distinct cellular components of the immune system. Modified from Montesinos et al, and Ma et al. 19,66","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"4 1","pages":"41-58"},"PeriodicalIF":0.2,"publicationDate":"2017-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.3.441","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48419714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Zamora-Zamora, J. L. Figueroa-Velasco, J. L. Cordero-Mora, Rafael Nieto-Aquino, A. García-Contreras, M. T. Sánchez-Torres, S. Carrillo-Domínguez, J. A. Martínez-Aispuro
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Zamora-Zamora V, Figueroa-Velasco JL, Cordero-Mora JL, Nieto-Aquino R, Garcia-Contreras AC, Sanchez-Torres MT, Carrillo-Dominguez S, Martinez-Aispuro JA. Conjugated linoleic acid supplementation does not improve boar semen quality and does not change its fatty acid profile. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.387 . The aim of this study was to evaluate the effects of adding conjugated linoleic acid (CLA) to boar diets on semen producion and characteristics, the fatty acid profile of spermatozoa, and on testicle and boar carcass parameters. Ten lean-type terminal-line (Yorkshire x Landrace x Petrain) adult boars were randomly assigned to one of two treatments: (1) a control diet and (2) the same diet with 1% CLA. The research was conducted in Mexico, and the animals were exposed to ambient temperatures betwen 13.5 and 26.3 °C. Boars were ejaculated twice per week a 12-week period to examine semen production and characteristics. The results suggest that supplementation of boar diets with CLA did not affect the spermatozoa fatty acid profile, semen volume, sperm concetration, viability, or motility, nor did it cause abnormal sperm morphology. Compared to controls, boar fed a CLA diet had a higher seminal pH and increased backfat thickness. In summary, this study indicates that CLA addition in boar diet does not change the semen characteristics of fatty acid profile of spermatozoa; however, it increases backfat thickness in sexually mature boars.
Veterinaria Mexico OA ISSN:2448-6760引用如下：Zamora Zamora V，Figueroa Velasco JL，Cordero Mora JL，Nieto Aquino R，Garcia Contreras AC，Sanchez Torres MT，Carrillo Dominguez S，Martinez Aispuro JA。添加共轭亚油酸不会改善公猪精液的质量，也不会改变其脂肪酸组成。兽医墨西哥OA。2017年；4（3）。doi:10.21753/vmoa.4.3.387。本研究的目的是评估在公猪日粮中添加共轭亚油酸（CLA）对精液生产和特性、精子脂肪酸特征以及睾丸和公猪胴体参数的影响。将10头瘦肉型终端系（Yorkshire x Landrace x Petrain）成年公猪随机分配到两种处理中的一种：（1）对照日粮和（2）含1%CLA的相同日粮。这项研究在墨西哥进行，动物暴露在13.5至26.3°C的环境温度下。猪每周射精两次，为期12周，以检查精液的产生和特征。结果表明，在公猪日粮中添加CLA不会影响精子脂肪酸、精液量、精子浓度、活力或运动能力，也不会导致精子形态异常。与对照组相比，喂食CLA饮食的公猪具有更高的精液pH值，并增加了背板厚度。总之，本研究表明，在公猪日粮中添加CLA不会改变精子的脂肪酸特征；然而，它增加了性成熟公猪的后腹肌厚度。
{"title":"Conjugated linoleic acid supplementation does not improve boar semen quality and does not change its fatty acid profile","authors":"V. Zamora-Zamora, J. L. Figueroa-Velasco, J. L. Cordero-Mora, Rafael Nieto-Aquino, A. García-Contreras, M. T. Sánchez-Torres, S. Carrillo-Domínguez, J. A. Martínez-Aispuro","doi":"10.21753/VMOA.4.3.387","DOIUrl":"https://doi.org/10.21753/VMOA.4.3.387","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Zamora-Zamora V, Figueroa-Velasco JL, Cordero-Mora JL, Nieto-Aquino R, Garcia-Contreras AC, Sanchez-Torres MT, Carrillo-Dominguez S, Martinez-Aispuro JA. Conjugated linoleic acid supplementation does not improve boar semen quality and does not change its fatty acid profile. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.387 . The aim of this study was to evaluate the effects of adding conjugated linoleic acid (CLA) to boar diets on semen producion and characteristics, the fatty acid profile of spermatozoa, and on testicle and boar carcass parameters. Ten lean-type terminal-line (Yorkshire x Landrace x Petrain) adult boars were randomly assigned to one of two treatments: (1) a control diet and (2) the same diet with 1% CLA. The research was conducted in Mexico, and the animals were exposed to ambient temperatures betwen 13.5 and 26.3 °C. Boars were ejaculated twice per week a 12-week period to examine semen production and characteristics. The results suggest that supplementation of boar diets with CLA did not affect the spermatozoa fatty acid profile, semen volume, sperm concetration, viability, or motility, nor did it cause abnormal sperm morphology. Compared to controls, boar fed a CLA diet had a higher seminal pH and increased backfat thickness. In summary, this study indicates that CLA addition in boar diet does not change the semen characteristics of fatty acid profile of spermatozoa; however, it increases backfat thickness in sexually mature boars.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"4 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.3.387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42673974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Zarza, E. Martínez‐Meyer, G. Suzán, G. Ceballos
Climate change may modify the spatial distribution of reservoirs hosting emerging and reemerging zoonotic pathogens, and forecasting these changes is essential for developing prevention and adaptation strategies. The most important reservoir of bovine paralytic rabies in tropical countries, is the vampire bat (Desmodus rotundus). In Mexico, the cattle industry loses more than $2.6 million US dollar, annually to this infectious disease. Therefore, we predicted the change in the distribution of D. rotundus due to future climate change scenarios, and examined the likely effect that the change in its distribution will have on paralytic rabies infections in Mexico. We used the correlative maximum entropy based model algorithm to predict the potential distribution of D. rotundus. Consistent with the literature, our results showed that temperature was the variable most highly associated with the current distribution of vampire bats. The highest concentration of bovine rabies was in Central and Southeastern Mexico, regions that also have high cattle population densities. Furthermore, our climatic envelope models predicted that by 2050–2070, D. rotundus will lose 20 % of its current distribution while the northern and central regions of Mexico will become suitable habitats for D. rotundus. Together, our study provides an advanced notice of the likely change in spatial patterns of D. rotundus and bovine paralytic rabies, and presents an important tool for strengthening the National Epidemiological Surveillance System and Monitoring programmes, useful for establishing holistic, long-term strategies to control this disease in Mexico.
{"title":"Geographic distribution of Desmodus rotundus in Mexico under current and future climate change scenarios: Implications for bovine paralytic rabies infection","authors":"H. Zarza, E. Martínez‐Meyer, G. Suzán, G. Ceballos","doi":"10.21753/VMOA.4.3.390","DOIUrl":"https://doi.org/10.21753/VMOA.4.3.390","url":null,"abstract":"Climate change may modify the spatial distribution of reservoirs hosting emerging and reemerging zoonotic pathogens, and forecasting these changes is essential for developing prevention and adaptation strategies. The most important reservoir of bovine paralytic rabies in tropical countries, is the vampire bat (Desmodus rotundus). In Mexico, the cattle industry loses more than $2.6 million US dollar, annually to this infectious disease. Therefore, we predicted the change in the distribution of D. rotundus due to future climate change scenarios, and examined the likely effect that the change in its distribution will have on paralytic rabies infections in Mexico. We used the correlative maximum entropy based model algorithm to predict the potential distribution of D. rotundus. Consistent with the literature, our results showed that temperature was the variable most highly associated with the current distribution of vampire bats. The highest concentration of bovine rabies was in Central and Southeastern Mexico, regions that also have high cattle population densities. Furthermore, our climatic envelope models predicted that by 2050–2070, D. rotundus will lose 20 % of its current distribution while the northern and central regions of Mexico will become suitable habitats for D. rotundus. Together, our study provides an advanced notice of the likely change in spatial patterns of D. rotundus and bovine paralytic rabies, and presents an important tool for strengthening the National Epidemiological Surveillance System and Monitoring programmes, useful for establishing holistic, long-term strategies to control this disease in Mexico.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"4 1","pages":"10-25"},"PeriodicalIF":0.2,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.3.390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46108121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Ramírez, S. M. G. Camacho, I. Ramírez, I. E. Verdugo, N. C. Campo, H. Moreno
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Hernandez Ramirez CV, Gaxiola Camacho SM, Osuna Ramirez I, Enriquez Verdugo I, Castro del Campo N, Lopez Moreno HS. Prevalence and risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa. Veterinaria Mexico OA 2017;4(2).doi: 10.21753/vmoa.4.2.369 Domestic dogs transmit Leptospira spp. to humans, and determining the health risk that they represent is of paramount importance. To determine the seroprevalence and main risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa, we obtained serum samples from 165 dogs. The samples were stored at -40 °C and were analysed by the microbiology laboratory at Centro Nacional de Sanidad Animal using the leptospirosis microscopic agglutination test. Additionally, a survey was performed to identify epidemiological risk factors, and statistical inference was determined using chi-square test, odd ratios, and logistic regression with a statistical significance set at P < 0.05. The prevalence of Leptospira was 9 % (15/165), and we identified seven serovars: canicola 17 (46 %), icterohaemorrhagiae (40 %), bratislava (40 %), grippotyphosa (33 %), shermani (33 %), pyrogenes (20 %) and ballum (13 %). Based on our epidemiological survey, the risk factors associated with the detection of antibodies against Leptospira include the permanent habitation of pets in courtyards (OR = 4.6, P < 0.05) and presence of water stored in drums and basins (OR = 3.25, P < 0.05). The prevalence of leptospirosis in dogs indicates that the disease is present in the city of Culiacan and that leptospiral antibodies in dogs increase in poor sanitary conditions with stored water, which increases the potential risk of infection for both humans and animals. Figure 1. Frequency of observed serovars of Leptospira
兽医墨西哥OA ISSN:2448-6760引用如下：Hernandez Ramirez CV，Gaxiola Camacho SM，Osuna Ramirez I，Enriquez Verdugo I，Castro del Campo N，Lopez Moreno HS。锡那罗亚州库利亚坎市犬钩端螺旋体血清型的患病率和相关危险因素。兽医墨西哥OA 2017；4（2）.doi:10.21753/vmoa.4.2.369家养狗将钩端螺旋体属传染给人类，确定它们所代表的健康风险至关重要。为了确定锡那罗亚州库利亚坎市狗钩端螺旋体血清型的血清流行率和主要危险因素，我们从165只狗身上采集了血清样本。样品储存在-40°C下，由国家卫生中心动物微生物学实验室使用钩端螺旋体病显微镜凝集试验进行分析。此外，还进行了一项调查，以确定流行病学风险因素，并使用卡方检验、奇数比和逻辑回归确定统计推断，统计显著性设置为P<0.05。钩端螺旋体的患病率为9%（15/165），我们确定了7种血清型：犬科17型（46%）、黄疸出血型（40%）、布拉迪斯拉瓦型（40%。根据我们的流行病学调查，与钩端螺旋体抗体检测相关的风险因素包括宠物是否长期居住在庭院中（OR=4.6，P<0.05）以及是否有水储存在桶和盆中（OR=3.25，P<0.05）储存水的条件，这增加了人类和动物感染的潜在风险。图1。观察到的钩端螺旋体血清变异的频率
{"title":"Prevalence and risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa","authors":"C. Ramírez, S. M. G. Camacho, I. Ramírez, I. E. Verdugo, N. C. Campo, H. Moreno","doi":"10.21753/VMOA.4.2.369","DOIUrl":"https://doi.org/10.21753/VMOA.4.2.369","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Hernandez Ramirez CV, Gaxiola Camacho SM, Osuna Ramirez I, Enriquez Verdugo I, Castro del Campo N, Lopez Moreno HS. Prevalence and risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa. Veterinaria Mexico OA 2017;4(2).doi: 10.21753/vmoa.4.2.369 Domestic dogs transmit Leptospira spp. to humans, and determining the health risk that they represent is of paramount importance. To determine the seroprevalence and main risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa, we obtained serum samples from 165 dogs. The samples were stored at -40 °C and were analysed by the microbiology laboratory at Centro Nacional de Sanidad Animal using the leptospirosis microscopic agglutination test. Additionally, a survey was performed to identify epidemiological risk factors, and statistical inference was determined using chi-square test, odd ratios, and logistic regression with a statistical significance set at P < 0.05. The prevalence of Leptospira was 9 % (15/165), and we identified seven serovars: canicola 17 (46 %), icterohaemorrhagiae (40 %), bratislava (40 %), grippotyphosa (33 %), shermani (33 %), pyrogenes (20 %) and ballum (13 %). Based on our epidemiological survey, the risk factors associated with the detection of antibodies against Leptospira include the permanent habitation of pets in courtyards (OR = 4.6, P < 0.05) and presence of water stored in drums and basins (OR = 3.25, P < 0.05). The prevalence of leptospirosis in dogs indicates that the disease is present in the city of Culiacan and that leptospiral antibodies in dogs increase in poor sanitary conditions with stored water, which increases the potential risk of infection for both humans and animals. Figure 1. Frequency of observed serovars of Leptospira","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"4 1","pages":"32-43"},"PeriodicalIF":0.2,"publicationDate":"2017-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.2.369","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45255360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Prado, F. M. Palacios, R. C. Sedano, H. M. Larios
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Cisneros Prado JL, Montiel Palacios F, Canseco Sedano R, Merchant Larios H. Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity. Veterinaria Mexico OA. 2017;4(2). doi: 10.21753/vmoa.4.2.424 . Ovaries from slaughtered cows are the most abundant source of preantral immature follicles for in vitro studies including maturation and in vitro fertilization. Integrity of immature follicles may be compromised by inadequate management during the collection and transport of the gonads, limiting the success of a correct evaluation. This study assessed the effect of aseptic and transport of bovine ovaries on the structural and ultrastructural integrity of immature follicles from the ovarian cortex. Ovaries from 20 different crossbred cows ( Bos taurus × B. indicus ) were randomly assigned to one of two aseptic and transport treatments: (1) the ovaries were washed once with 70 % ethanol for 10 s, rinsed twice with normal saline solution (OH + NSS), and then transported in NSS + 0.05 % gentamicin sulphate; or (2) the ovaries were washed three times with phosphate buffered saline (PBS) and transported in PBS + 0.05 % gentamicin sulphate. Samples of the ovarian cortex were obtained from each treatment group. Half of samples of each treatment was processed for histologic evaluation of follicular morphology and developmental stage using haematoxylin & eosin, and the other half was processed to analyse the ultrastructure of immature follicles using transmission electronic microscopy. The rates of good-quality follicles were 36.95 % and 88.6 % for OH + NSS and PBS, respectively. Histological analysis showed higher number of abnormal follicles in OH + NSS than in PBS treated ovaries (F 1,12 = 99.64, P = 0.0001). The proportions of morphological alterations were not significantly different between treatments (χ 2 1,7 = 7.72, P = 0.358). The ultrastructure of immature follicles from OH + NSS ovaries showed cell damage, revealed by abundant cell detritus around both, the oocyte and follicular cells. However, the presence of alterations was barely evident in both the cytoplasm and the nucleus of the granulosa cells. Figure 2. Semi-fine cuts of immature follicles present in bovine ovarian cortex treated with PBS (A, B, C; viewed at 40X) or OH+NSS (D, E, F; viewed at 40X).
{"title":"Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity","authors":"J. Prado, F. M. Palacios, R. C. Sedano, H. M. Larios","doi":"10.21753/VMOA.4.2.424","DOIUrl":"https://doi.org/10.21753/VMOA.4.2.424","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Cisneros Prado JL, Montiel Palacios F, Canseco Sedano R, Merchant Larios H. Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity. Veterinaria Mexico OA. 2017;4(2). doi: 10.21753/vmoa.4.2.424 . Ovaries from slaughtered cows are the most abundant source of preantral immature follicles for in vitro studies including maturation and in vitro fertilization. Integrity of immature follicles may be compromised by inadequate management during the collection and transport of the gonads, limiting the success of a correct evaluation. This study assessed the effect of aseptic and transport of bovine ovaries on the structural and ultrastructural integrity of immature follicles from the ovarian cortex. Ovaries from 20 different crossbred cows ( Bos taurus × B. indicus ) were randomly assigned to one of two aseptic and transport treatments: (1) the ovaries were washed once with 70 % ethanol for 10 s, rinsed twice with normal saline solution (OH + NSS), and then transported in NSS + 0.05 % gentamicin sulphate; or (2) the ovaries were washed three times with phosphate buffered saline (PBS) and transported in PBS + 0.05 % gentamicin sulphate. Samples of the ovarian cortex were obtained from each treatment group. Half of samples of each treatment was processed for histologic evaluation of follicular morphology and developmental stage using haematoxylin & eosin, and the other half was processed to analyse the ultrastructure of immature follicles using transmission electronic microscopy. The rates of good-quality follicles were 36.95 % and 88.6 % for OH + NSS and PBS, respectively. Histological analysis showed higher number of abnormal follicles in OH + NSS than in PBS treated ovaries (F 1,12 = 99.64, P = 0.0001). The proportions of morphological alterations were not significantly different between treatments (χ 2 1,7 = 7.72, P = 0.358). The ultrastructure of immature follicles from OH + NSS ovaries showed cell damage, revealed by abundant cell detritus around both, the oocyte and follicular cells. However, the presence of alterations was barely evident in both the cytoplasm and the nucleus of the granulosa cells. Figure 2. Semi-fine cuts of immature follicles present in bovine ovarian cortex treated with PBS (A, B, C; viewed at 40X) or OH+NSS (D, E, F; viewed at 40X).","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"4 1","pages":"19-31"},"PeriodicalIF":0.2,"publicationDate":"2017-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.2.424","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48487850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. A. L. Mejía, Pablo Alfonso González Lomelín, H. L. Butron, R. D. Rubio, S. R. L. Hernández, I. Valdéz, V. Hernández
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Lomeli Mejia PA, Gonzalez Lomelin PA, Lecona Butron H, Dominguez Rubio R, Leon Hernandez SR, Luna Valdez IX, Dominguez Hernandez V. Quantitative evaluation of osteogenesis through infrared light. Pilot study. Veterinaria Mexico OA. 2017;4(2). doi: 10.21753/vmoa.4.2.410 . Different methods are available to evaluate the degree of bone healing. A good choice involves employing optical techniques with infrared light to eval-uate the progress of bone consolidation. Because infrared light is absorbed in liquids and reflected in solids, it is possible to assess bone consolidation progress using an incident light source and a coupled photo sensor. We used a 940-nm light source that is capable of reaching bone to determine the degree of bone consolidation. Five New Zealand White rabbits were used according to the NOM-062-ZOO99 standard. In each animal, a fracture was generated in the left tibia, and the fragments were fixed using an external fixator constructed with Kirschner nails and dental acrylic cement. Progress in bone consolidation was evaluated at days 7, 23, and 34 after surgery. A linear dependence was observed between the days elapsed and the reflection of the infrared light. Figure 2. Reduction of tibia by external fixators made with Kirschner nails 0.045 inch in diameter and methylmethacrylate cement.
{"title":"Quantitative evaluation of osteogenesis through infrared light: Pilot study","authors":"P. A. L. Mejía, Pablo Alfonso González Lomelín, H. L. Butron, R. D. Rubio, S. R. L. Hernández, I. Valdéz, V. Hernández","doi":"10.21753/VMOA.4.2.410","DOIUrl":"https://doi.org/10.21753/VMOA.4.2.410","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Lomeli Mejia PA, Gonzalez Lomelin PA, Lecona Butron H, Dominguez Rubio R, Leon Hernandez SR, Luna Valdez IX, Dominguez Hernandez V. Quantitative evaluation of osteogenesis through infrared light. Pilot study. Veterinaria Mexico OA. 2017;4(2). doi: 10.21753/vmoa.4.2.410 . Different methods are available to evaluate the degree of bone healing. A good choice involves employing optical techniques with infrared light to eval-uate the progress of bone consolidation. Because infrared light is absorbed in liquids and reflected in solids, it is possible to assess bone consolidation progress using an incident light source and a coupled photo sensor. We used a 940-nm light source that is capable of reaching bone to determine the degree of bone consolidation. Five New Zealand White rabbits were used according to the NOM-062-ZOO99 standard. In each animal, a fracture was generated in the left tibia, and the fragments were fixed using an external fixator constructed with Kirschner nails and dental acrylic cement. Progress in bone consolidation was evaluated at days 7, 23, and 34 after surgery. A linear dependence was observed between the days elapsed and the reflection of the infrared light. Figure 2. Reduction of tibia by external fixators made with Kirschner nails 0.045 inch in diameter and methylmethacrylate cement.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"4 1","pages":"1-8"},"PeriodicalIF":0.2,"publicationDate":"2017-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.2.410","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45824471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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