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Investigation of the Genotoxic Effects of the Analgesic Drugs Piroxicam and Tenoxicam in Human Lymphocytes 镇痛药吡罗昔康和替诺昔康对人淋巴细胞的遗传毒性研究
Pub Date : 2023-10-15 DOI: 10.34248/bsengineering.1340985
Hümeyra GÜL, Ece AVULOĞLU YILMAZ
Analgesics are one of the most widely used drug groups in the world. Since the genotoxic and carcinogenic effects of some analgesic drugs have been detected, studies investigating these effects have increased. In this study, the genotoxic effects of the analgesic drugs Tenoxicam and Piroxicam were investigated in vitro in human peripheral lymphocytes by chromosomal aberrations (CAs) and micronucleus (MN) assays. In addition, mitotic index (MI) and nuclear division index (NDI) values were also determined. As a result, no difference in chromosomal abnormalities was observed in both 24 and 48 hours of Piroxicam treatment. Micronucleus frequency was increased at the three highest concentrations (0.94, 1.88 and 3.75 µg/mL) compared to the control. Piroxicam significantly decreased MI compared to both control and solvent control at concentrations of 1.88 and 3.75 µg/mL at all exposure times. Tenoxicam showed a statistically significant increase at the highest concentration (10 μg/mL) compared to the control in the 24 h CA treatment. On the other hand, no effect was observed in 48-hour CA treatment. It was concluded that tenoxicam did not cause a toxic effect at any concentration except the highest concentration (10 μg/mL) in the MN assay results. Mitotic index (MI) significantly decreased at concentrations of 1.25, 2.5, 5 and 10 μg/mL. Nuclear division index (NDI) results did not change for both agents. In conclusion, both agents were found to be genotoxic only at high concentrations and the effect was weak. This study is pioneering as there have been no previous in vitro studies in human peripheral lymphocytes for both Piroxicam and Tenoxicam. These results need to be supported by different cell groups and in vivo assays.
镇痛药是世界上使用最广泛的药物之一。由于一些镇痛药物的遗传毒性和致癌性作用已被发现,因此对这些作用的研究有所增加。本研究采用染色体畸变(CAs)和微核(MN)方法研究了镇痛药物替诺昔康和吡罗西康体外对人外周血淋巴细胞的遗传毒性作用。测定了有丝分裂指数(MI)和核分裂指数(NDI)。结果,在吡罗昔康治疗的24和48小时内,染色体异常没有观察到差异。微核频率在3个最高浓度(0.94、1.88和3.75µg/mL)下较对照增加。在所有暴露时间,Piroxicam浓度分别为1.88和3.75µg/mL时,与对照组和溶剂对照组相比,MI均显著降低。在CA处理24 h时,替诺昔康的最高浓度(10 μg/mL)较对照组有统计学意义的升高。另一方面,在48小时的CA治疗中没有观察到任何效果。结果表明,除MN检测结果中最高浓度(10 μg/mL)外,替诺昔康在任何浓度下均无毒性作用。有丝分裂指数(MI)在1.25、2.5、5和10 μg/mL浓度下显著降低。两种药物的核分裂指数(NDI)结果均无变化。结果表明,两种药剂仅在高浓度时具有遗传毒性,且作用较弱。这项研究是开创性的,因为之前还没有对吡罗昔康和替诺昔康在人外周血淋巴细胞中的体外研究。这些结果需要不同的细胞组和体内实验的支持。
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引用次数: 0
Biodiversity of Actinobacteria Isolated from Marmara and Avşa Islands in Türkiye 斯里兰卡马尔马拉岛和av<e:1>岛放线菌的生物多样性
Pub Date : 2023-10-15 DOI: 10.34248/bsengineering.1355194
Ahmet Rıdvan TOPKARA, Kamil IŞIK
The main purpose of this study is to investigate the microbial diversity of Actinobacteria, living in soils in the “Marmara” and “Avşa” Islands and to identify species at genus level based on 16S rRNA gene sequences. These islands are located in the southwest of the Sea of Marmara and in the literature review, no Actinobacteria biodiversity studies related to Marmara and Avşa Islands were found. Such unexplored ecological habitats are potentially rich source for discovery sources of novel species and bioactive molecule. Actinobacteria play an important role in many natural phenomena such as nitrogen fixation, roles of these prokaryotic microorganisms. In this article, we focused on the presence and the diversity of Actinobacteria on the Islands by examining multiple sampling sites and using different selective isolation media. A total of 400 culturable Actinobacteria were isolated using ten different isolation media by dilution-plating method. Among the 400 isolates, 112 isolates were selected according to their morphology in different culture media. The isolates were characterized on the basis of 16S ribosomal RNA gene sequencing and phylogenetic analysis. The results showed a high level of actinobacterial diversity with 16 different genera. These genera obtained as a result of phylogenetic analyzes are Streptomyces, Nonomuraea, Nocardia, Actinomadura, Micromonospora, Kribbella, Mycolicibacterium, Microbispora, Saccharopolyspora, Jiangella, Rhodococcus, Actinopolymorpha, Geodermatophilus, Dactylosporangium, Pseudonocardia and Nocardioides. Many isolates are identified as new species by our current research. Findings from this study showed that the soil of Marmara and Avşa Islands can be a good source of isolation for Actinobacteria.
本研究的主要目的是研究“马尔马拉”岛和“av”岛土壤中放线菌的微生物多样性,并基于16S rRNA基因序列在属水平上进行物种鉴定。这些岛屿位于马尔马拉海西南部,文献综述中未发现与马尔马拉岛和av岛相关的放线菌生物多样性研究。这些未开发的生态栖息地是发现新物种和生物活性分子的潜在丰富来源。放线菌在许多自然现象中起着重要的作用,如固氮,这些原核微生物的作用。在这篇文章中,我们通过检查多个采样点和使用不同的选择性分离介质,重点研究了放线菌在岛屿上的存在和多样性。采用稀释镀法,采用10种不同的分离培养基,共分离出400株放线菌。根据菌株在不同培养基上的形态,从400株菌株中筛选出112株。通过16S核糖体RNA基因测序和系统发育分析对分离株进行了鉴定。结果表明,放线菌具有较高的多样性,有16个不同的属。系统发育分析得到的这些属是链霉菌属、诺卡菌属、诺卡菌属、放线瘤菌属、小单孢菌属、Kribbella菌属、分枝杆菌属、微双孢菌属、Saccharopolyspora菌属、江氏菌属、红球菌属、放线多孢菌属、嗜土菌属、Dactylosporangium菌属、假心菌属和诺卡菌属。根据我们目前的研究,许多分离株被确定为新种。本研究结果表明,马尔马拉岛和av岛的土壤可以成为放线菌的良好分离来源。
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引用次数: 0
Investigation of Vegetative Growth Characteristics of Some Vegetable Species in Brassicaceae Family 芸苔科几种蔬菜营养生长特性的研究
Pub Date : 2023-10-15 DOI: 10.34248/bsengineering.1357067
Dilek KANDEMİR, Kübra PALA, Güldane Tuğba ŞAHİN, Ahmet BALKAYA
Lahanagil sebze türleri kışlık sebzeler içerisinde önemli bir yere sahiptir. Olumsuz çevre koşulları bu sebze türlerinde büyüme ve gelişmeyi olumsuz düzeyde etkileyebilmekte ve verim unsurlarında önemli azalışlara neden olmaktadır. Bu araştırmada, bazı lahanagil türlerine ait çeşitlerde fide dikiminden itibaren belirli aralıklarla yapılan kantitatif analizlerle vejetatif büyüme özellikleri yönünden karşılaştırılması ve ortaya çıkan farklılıkların fizyolojik parametrelerle incelenmesi amaçlanmıştır. Çalışmada, brokoli, karnabahar, Brüksel lahana, yaprak lahana, beyaz baş lahana ve kırmızı baş lahana türleri kullanılmıştır. Fide dikim tarihini takiben 45. güne kadar 15 gün aralıklı yapılan kantitatif analizlerde yaprak sayısı (adet/bitki), gövde çapı (mm), bitki boyu (cm), toprak üstü aksam yaş ve kuru ağırlığı (g), kök yaş ve kuru ağırlığı (g), bitki yaş ağırlık kuru ağırlığı (g) değerleri belirlenmiştir. Ayrıca, oransal toprak üstü aksam ağırlığı ve oransal kök ağırlığı değerleri de incelenmiştir. Araştırma sonucunda fide dikiminden itibaren 45. günde yapılan kantitatif analiz sonucunda lahanagil türlerinin vejetatif büyüme özellikleri yönünden önemli düzeyde farklılıklar gösterdiği tespit edilmiştir. Lahana grubu sebze türlerinde yaprak sayısı 11-14 adet/bitki arasında değişim göstermiş ve baş lahanaların yaprak sayısının diğer türlere oranla daha fazla olduğu belirlenmiştir. Bitki boyu değerleri 36 - 47 cm aralıklarında dağılım göstermiş ve en yüksek brokoli türünde ölçülmüştür. Toprak üstü aksamı kuru ağırlık değeri 7.31-10.31 g arasında değişim göstermiştir. Araştırmada kök kuru ağırlık değerleri 0.80-1.73 g değerleri arasında dağılış göstermiş ve en yüksek kök kuru ağırlık değerleri sırasıyla yaprak lahana ve Brüksel lahanasında tespit edilmiştir. Oransal kök ağırlığının 0.09-0.14 arasında değiştiği saptanmıştır. Araştırma sonuçlarının, simülasyon çalışmaları ile gelecekte diğer farklı kışlık çeşitlerde kullanımı ve üretim planlanmasında faydalı olacağı öngörülmektedir.
卷心菜在冬季蔬菜中占有重要地位。不利的环境条件可能会对这些蔬菜品种的生长发育造成不利影响,并导致产量系数显著降低。本研究旨在通过从幼苗定植开始的一定时间间隔进行定量分析,比较一些卷心菜品种的无性生长特性,并研究其生理参数方面的差异。研究使用了西兰花、花椰菜、球芽甘蓝、叶甘蓝、白头甘蓝和红头甘蓝等品种。每隔 15 天进行一次定量分析,直至播种后第 45 天,并测定叶片数(片/株)、茎直径(毫米)、株高(厘米)、地上部分鲜重和干重(克)、根鲜重和干重(克)以及植株鲜重和干重(克)。此外,还分析了地上部分比例重量和根部比例重量值。定植后第 45 天进行的定量分析结果表明,白菜品种在无性生长特性方面存在显著差异。卷心菜类蔬菜品种的叶片数在 11-14 片/株之间变化,其中大头菜的叶片数高于其他品种。植株高度值在 36 - 47 厘米之间,西兰花的植株高度值最高。地上部分的干重介于 7.31-10.31 克之间。根部干重值分布在 0.80-1.73 克之间,叶菜和球芽甘蓝的根部干重值最高。根系重量比例值介于 0.09-0.14 之间。预计研究结果将有助于今后对其他不同冬季品种的使用和生产规划进行模拟研究。
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引用次数: 0
Elevating Thyme Species Identification: Exploiting Key Chloroplast Genes (matK, rbcL, and psbA-trnH) through DNA Barcoding and Phylogenetic Analysis 提升百里香物种鉴定:利用DNA条形码和系统发育分析挖掘叶绿体关键基因(matK、rbcL和psbA-trnH)
Pub Date : 2023-10-15 DOI: 10.34248/bsengineering.1352976
M. Alp FURAN
Understanding genetic relationships and diversity among species is crucial for unraveling evolutionary processes, ecological interactions, and conservation strategies. DNA sequence analysis serves as a powerful tool in this endeavor. This study focuses on the Thymus genus, a collection of notable species, to investigate its genetic framework. Leveraging DNA sequences from key regions (matK, rbcL, and psbA-trnH), we aim to elucidate genetic connections within the Thymus genus and uncover mechanisms driving its diversity. The Thymus genus, with its diverse species and ecological characteristics, provides a captivating platform for genetic exploration. Through DNA sequence analysis, we aim to unveil genetic interconnections, biodiversity patterns, and the factors shaping the genus's evolution. Our findings are aligned with previous studies, and this consistency highlights the presence of polymorphism within potential sequences. Employing coding loci and spacer regions, our study contributes to Lamiaceae family barcoding research. Despite variations across gene regions, the concatenation of sequences enhances result reliability. We analyzed the suitability of matK, rbcL, and psbA sequences for Thymus identification, observing rbcL and psbA outperforming matK. Our novel approach, rooted in chloroplast DNA, presents a promising method for species discernment. By analyzing multiple chloroplast gene regions, this technique offers a fresh perspective on genetic affinity assessment using DNA barcodes. In conclusion, this study not only contributes to Thymus germplasm resource preservation but also exemplifies a novel approach to discerning Thymus species through DNA analysis. This methodology carries the potential for broader application, enriching our understanding of genetic relationships and diversity in the plant kingdom.
了解物种之间的遗传关系和多样性对于揭示进化过程、生态相互作用和保护策略至关重要。DNA序列分析是这方面的有力工具。本研究的重点是胸腺属,一个著名的物种集合,研究其遗传框架。利用来自关键区域(matK, rbcL和psbA-trnH)的DNA序列,我们的目标是阐明胸腺属的遗传联系并揭示驱动其多样性的机制。胸腺属植物以其多样性和生态特性,为基因探索提供了一个迷人的平台。通过DNA序列分析,我们旨在揭示遗传联系,生物多样性模式,以及塑造属进化的因素。我们的发现与以前的研究一致,这种一致性突出了潜在序列中多态性的存在。利用编码位点和间隔区,为Lamiaceae科的条形码研究做出了贡献。尽管基因区域之间存在差异,序列的串联提高了结果的可靠性。我们分析了matK、rbcL和psbA序列在胸腺鉴定中的适用性,观察到rbcL和psbA序列优于matK序列。我们的新方法,根植于叶绿体DNA,提出了一种很有前途的物种识别方法。该技术通过对叶绿体多个基因区域的分析,为利用DNA条形码进行遗传亲和性评价提供了新的视角。总之,本研究不仅有助于胸腺种质资源的保存,而且为胸腺物种的DNA鉴定提供了新的途径。这种方法具有更广泛应用的潜力,丰富了我们对植物界遗传关系和多样性的理解。
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引用次数: 0
3 Boyutlu görüntüleme kullanılarak bir ağaç gövde çap ölçüm uygulamasının geliştirilmesi 利用 3D 成像技术开发树干直径测量应用程序
Pub Date : 2023-10-15 DOI: 10.34248/bsengineering.1352585
Hayrettin KARADÖL, Mehmet GÖK, Mehmet TEKEREK
Orman işletmelerinde sürekli ve rasyonel bir çalışma gerçekleştirebilmek için ağaçların odun hacim ve artım miktarının periyodik olarak belirlenmesi önemlidir. Ağaçlardan elde edilecek materyallerin farklı kullanım amaçları için ağaçların odun hacimlerini belirlemede ağaç gövde çapının hassas bir şekilde ölçülmesi gerekmektedir. Ağaç gövde çapları çoğunlukla manuel olarak kumpaslarla ölçülmektedir. Bu işlem sürecinde bir orman işçisi kumpasla ağacın gövde çapını ölçmekte ve başka bir işçi ise bu ölçüm bilgisini dikili ağaç ölçüm tutanağına kaydetmekte ve daha sonra analiz için bu veriler bilgisayar ortamına aktarılmaktadır. Tüm bu süreçler zaman, iş gücü ve ölçüm hataları gibi sorunları da beraberinde getirmektedir. Bu çalışmada, ağaç gövde çaplarını ölçebilmek amacıyla derinlik bilgisi içeren bir kamera, tek kart bilgisayar ve diğer çevre birimlerden oluşan düşük maliyetli taşınabilir bir ölçüm sisteminin tasarımı ortaya konmuş ve ön testleri gerçekleştirmek amacıyla deneysel bir sistem oluşturulmuştur. Hedef plan doğrultusunda oluşturulan görüntüleme sistemi ile bazı ön testler gerçekleştirilmiştir. Derinlik bilgisi içeren görüntülerde, hedeflenen bir ağaç gövdesinin dış aydınlatma ortamından etkilenmeksizin ön plana çıkarabiliyor olması, ağaç gövde çapının hassas bir şekilde belirlenmesini sağlamaktadır. Kamera sensörü-ağaç gövdesi arası mesafe belirli bir sınır değer içerisinde (20-100cm) olması koşuluyla 15 farklı ölçüm gerçekleştirilmiş ve manuel kumpas ölçümüne göre ağaç gövde çapları maksimum 1.975 cm hata değeri ile belirlenmiştir.
为了实现林业企业的持续合理运营,定期确定树木的材积和增量非常重要。在确定树木的木材蓄积量以满足从树木中获取材料的不同用途时,有必要精确测量树干直径。树干直径大多是用卡尺人工测量的。在这一过程中,林业工人用卡尺测量树干直径,另一名工人将测量信息记录在活立木测量记录中,然后将这些数据传输到计算机环境中进行分析。所有这些过程都会带来时间、劳动力和测量误差等问题。本研究提出了一种低成本便携式测量系统的设计方案,该系统由一台带深度信息的照相机、一台单板计算机和其他外围设备组成,用于测量树干直径,并创建了一个实验系统进行初步测试。根据目标计划创建的成像系统进行了一些初步测试。在包含深度信息的图像中,目标树干可以不受外部光照环境的影响而被突出显示,从而可以精确测定树干直径。在相机传感器与树干之间的距离在一定限值(20-100 厘米)范围内的情况下,进行了 15 次不同的测量,根据人工卡尺测量,确定的树干直径的最大误差值为 1.975 厘米。
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引用次数: 0
Scattering from Parabolic Cylindrical Reflector Antenna Using Modified Theory of Optics 基于修正光学理论的抛物面柱面反射天线散射
Pub Date : 2023-10-15 DOI: 10.34248/bsengineering.1338995
Fadıl KUYUCUOĞLU
In this study, numerical analysis of scattering from a dielectric-coated metallic reflector is presented. The reflector has a parabolic cross-sectional cylindrical geometry. Radiation patterns and aperture efficiency parameters are analyzed using physical optics. A complex source point located at the focal point of the parabolic reflector is used as the source of radiation. The dielectric-coated metallic reflector is considered as an antenna, and an impedance boundary is utilized. The effects of coating on radiation patterns are analyzed in terms of dielectric thickness and dielectric permittivity. Numerical results are presented for various parameters, including dielectric permittivity, dielectric layer thickness, complex source point width, and focal distance.
本文对介质涂层金属反射器的散射进行了数值分析。反射器具有抛物线横截面圆柱形几何形状。利用物理光学分析了辐射模式和孔径效率参数。位于抛物面反射器焦点处的复杂源点被用作辐射源。将介质涂层金属反射器视为天线,并利用阻抗边界。从介质厚度和介电常数的角度分析了涂层对辐射图的影响。给出了介电常数、介电层厚度、复源点宽度和焦距等参数的数值计算结果。
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引用次数: 0
Opti-Waffle: A Technological Furniture Design and Manufacturing Model Opti-Waffle:一种工艺家具设计与制造模式
Pub Date : 2023-10-15 DOI: 10.34248/bsengineering.1344142
Erdem YILDIRIM
Parametric design allows the use of computers and systems that can make decisions beyond human capacity, such as machine learning, through optimization in design and manufacturing. From this point of view, it is aimed to shape and manufacture the design by minimizing the subjective decisions of the designers by using various algorithmic methods and structural optimization to provide ergonomics in a furniture design. As the subject of the study, a meeting table for 8 people was discussed. In the process, 'artificial intelligence supported inspiration board', 'parametric design', 'human-computer interaction and sensors', 'topology optimization', 'observation in augmented reality' and 'computer-aided manufacturing' techniques were used sequentially. After the assembly was completed, the product obtained was finally evaluated in terms of structure-function relationship.
参数化设计允许使用计算机和系统,这些计算机和系统可以通过优化设计和制造来做出超出人类能力的决策,例如机器学习。从这个角度来看,它的目的是通过使用各种算法方法和结构优化,通过最大限度地减少设计师的主观决策来塑造和制造设计,从而在家具设计中提供人体工程学。作为研究的对象,我们讨论了一张8人的会议桌。在此过程中,“人工智能支持的灵感板”、“参数化设计”、“人机交互与传感器”、“拓扑优化”、“增强现实观察”和“计算机辅助制造”技术被依次使用。装配完成后,最后对得到的产品进行结构-功能关系评价。
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引用次数: 0
Maximum Likelihood Estimation for the Log-Logistic Distribution Using Whale Optimization Algorithm with Applications 基于鲸鱼优化算法的logistic分布的最大似然估计及其应用
Pub Date : 2023-10-15 DOI: 10.34248/bsengineering.1356036
Adi Omaia FAOURİ, Pelin KASAP
The log-logistic distribution has been widely used in several fields, including engineering, survival analysis, and economics. The method of maximum likelihood estimation is used in this study for estimating the shape and scale parameters for the log-logistic distribution, whereas in the case of the log-logistic distribution, likelihood equations lack explicit solutions. Therefore, problems with solving likelihood equations can be solved by using two highly efficient algorithms, which are the whale optimization algorithm and the Nelder-Mead algorithm, as well as by showing the applicability of this distribution by comparing it with other well-known classical distributions. To demonstrate the performance of each algorithm implemented, an extensive Monte Carlo simulation study has been conducted. The performance of maximum likelihood estimators for each algorithm has been evaluated in terms of mean square error and deficiency criteria. It has been seen that the whale optimization algorithm provides the best estimates for the log-logistic distribution parameters according to the simulation data.
物流分布在工程、生存分析、经济学等多个领域得到了广泛的应用。本研究使用极大似然估计方法来估计对数-logistic分布的形状和尺度参数,而在对数-logistic分布的情况下,似然方程缺乏显式解。因此,求解似然方程的问题可以通过使用鲸鱼优化算法和Nelder-Mead算法这两种高效的算法来解决,并通过与其他著名的经典分布进行比较来展示该分布的适用性。为了演示所实现的每个算法的性能,进行了广泛的蒙特卡罗模拟研究。根据均方误差和缺陷准则对每种算法的最大似然估计器的性能进行了评估。已经看到,鲸鱼优化算法根据仿真数据提供了对数-逻辑分布参数的最佳估计。
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引用次数: 0
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Black sea journal of engineering and science
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