Soledad Ruiz P, Cecilia Baumberger, Pedro Jimenez-Bluhm, V. Marambio, C. Salazar, C. Hamilton-West
Research and interest in avian influenza virus have increased considerably in recent decades in response to highly pathogenic avian influenza outbreaks in poultry and its zoonotic potential. Wild waterfowl are the main reservoir of the virus, therefore studying the dynamics of influenza A virus (IAV) infection in these populations is essential in order to understand its potential persistence in the environment and transmission to poultry and humans. It has been identified that environmental factors (such as temperature, rainfall, vegetation and landscape characteristics, among others) can play an important role in the maintenance and dissemination of the virus in the areas of concentration of wild birds. However, studies that include ecological aspects of the virus and explore the interaction between the prevalence of IAV in wild birds and environmental factors remain scarce. This review summarizes research efforts that have been made to identify the environmental factors involved in the persistence and transmission of IAV in areas of wild bird concentration and how these factors may influence the prevalence of the virus in these populations, generating differences in the presentation of the infection among different geographical areas.
{"title":"Factores ambientales relacionados con la presentación de virus influenza A en aves silvestes","authors":"Soledad Ruiz P, Cecilia Baumberger, Pedro Jimenez-Bluhm, V. Marambio, C. Salazar, C. Hamilton-West","doi":"10.21897/rmvz.1845","DOIUrl":"https://doi.org/10.21897/rmvz.1845","url":null,"abstract":"Research and interest in avian influenza virus have increased considerably in recent decades in response to highly pathogenic avian influenza outbreaks in poultry and its zoonotic potential. Wild waterfowl are the main reservoir of the virus, therefore studying the dynamics of influenza A virus (IAV) infection in these populations is essential in order to understand its potential persistence in the environment and transmission to poultry and humans. It has been identified that environmental factors (such as temperature, rainfall, vegetation and landscape characteristics, among others) can play an important role in the maintenance and dissemination of the virus in the areas of concentration of wild birds. However, studies that include ecological aspects of the virus and explore the interaction between the prevalence of IAV in wild birds and environmental factors remain scarce. This review summarizes research efforts that have been made to identify the environmental factors involved in the persistence and transmission of IAV in areas of wild bird concentration and how these factors may influence the prevalence of the virus in these populations, generating differences in the presentation of the infection among different geographical areas.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48269727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniela Niño-Miranda, Mario Alejandro Córdoba-Martínez, Luz Marina Arias-Reyes, J. Quirós-Rodríguez
Objective. Evaluate the current status of Melongena melongena in Cispata Bay, Colombian Caribbean through the population aspects of the species. Materials and methods. Three sampling sectors were established, according to the zoning of the mangrove in the bay. For the collection of the biological material a linear transect of 4 x 20 m was located perpendicular to the area of the mangrove with two replicas at a distance of 50 m. For the determination of sex, a subsample of 10 individuals with sizes greater than that estimated for the sexual maturity of the species was taken. The shells were measured in total length, in order to differentiate the sizes of females and males. In each sector, the water temperature and salinity were recorded in situ, using an Extech EC170 multi-parameter meter, and the transparency of the water was calculated using the Secchi disk. Results. A total of 1,149 individuals of M. melongena were recorded, obtaining the highest abundance in the Cano Salado sector (522), followed by Las Cagas (458) and finally Amaya (169). 63% of the individuals were in a size range between 41-61 mm. The sex ratio was 1:1.2 (H:M). The sizes recorded in females were from 55 to 92 mm and in males from 54 to 77 mm. Conclusion. M. melongena in Cispata Bay has been showing evident signs of recovery in the last decade, considering that most registered individuals have reached the average height of sexual maturity.
{"title":"Estado actual de Melongena melongena (Mollusca: Gastropoda) en la bahía de Cispatá, Caribe Colombiano","authors":"Daniela Niño-Miranda, Mario Alejandro Córdoba-Martínez, Luz Marina Arias-Reyes, J. Quirós-Rodríguez","doi":"10.21897/rmvz.1873","DOIUrl":"https://doi.org/10.21897/rmvz.1873","url":null,"abstract":"Objective. Evaluate the current status of Melongena melongena in Cispata Bay, Colombian Caribbean through the population aspects of the species. Materials and methods. Three sampling sectors were established, according to the zoning of the mangrove in the bay. For the collection of the biological material a linear transect of 4 x 20 m was located perpendicular to the area of the mangrove with two replicas at a distance of 50 m. For the determination of sex, a subsample of 10 individuals with sizes greater than that estimated for the sexual maturity of the species was taken. The shells were measured in total length, in order to differentiate the sizes of females and males. In each sector, the water temperature and salinity were recorded in situ, using an Extech EC170 multi-parameter meter, and the transparency of the water was calculated using the Secchi disk. Results. A total of 1,149 individuals of M. melongena were recorded, obtaining the highest abundance in the Cano Salado sector (522), followed by Las Cagas (458) and finally Amaya (169). 63% of the individuals were in a size range between 41-61 mm. The sex ratio was 1:1.2 (H:M). The sizes recorded in females were from 55 to 92 mm and in males from 54 to 77 mm. Conclusion. M. melongena in Cispata Bay has been showing evident signs of recovery in the last decade, considering that most registered individuals have reached the average height of sexual maturity.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44693101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Medrano-Galarza, A. Zúñiga-López, Fredy García-Castro
Objective. The aim of this study was to determine an overall on-farm animal welfare score (WS) for dairy farms using a protocol involving animal- and management/resource-based variables; and to identify the variables that contributed the most to the variance for the WS. Materials and methods. Twenty-five pasture-based dairy farms in Savanna of Bogota - Colombian high tropic, were visited twice. During each visit, a total of 15 animal- and 13 management/resource-based variables were evaluated. For each variable, a herd-level negative cut-off point was established to determine compliance. Based on compliance, a WS was determined (from 0 to 100) and farms were classified as 'excellent', 'enhanced', 'acceptable', or 'non-acceptable'. A linear regression model was used to evaluate the association between variables at the herd-level with the WS. Results. Overall, median WS was 82 (min=67; max=97). Two farms were classified as 'excellent' (WS=94.5), 20 as 'enhanced' (WS=82.5), and 3 as 'acceptable' (WS=68). Among all variables, the noncompliance regarding subclinical mastitis, hind-legs and udder dirtiness score, ventral line wounds, Haematobia irritans fly counts, and condition/cleanliness of pre-milking holding area was significantly associated with a 5.6, 3.6, 6.7, 5.1, 6.5, and 6.1 points reduction in the WS, respectively (R2=0.84). Conclusions. Welfare level found across farms was good and encouraging. Nonetheless, six variables were identified as the main contributors to an unfavorable result. Strategies for improvement could focus on these variables to be able to reach a welfare level of ‘excellence’.
{"title":"Animal welfare assessment on pasture-based dairy farms in Savanna of Bogota, Colombia","authors":"C. Medrano-Galarza, A. Zúñiga-López, Fredy García-Castro","doi":"10.21897/rmvz.1708","DOIUrl":"https://doi.org/10.21897/rmvz.1708","url":null,"abstract":"Objective. The aim of this study was to determine an overall on-farm animal welfare score (WS) for dairy farms using a protocol involving animal- and management/resource-based variables; and to identify the variables that contributed the most to the variance for the WS. Materials and methods. Twenty-five pasture-based dairy farms in Savanna of Bogota - Colombian high tropic, were visited twice. During each visit, a total of 15 animal- and 13 management/resource-based variables were evaluated. For each variable, a herd-level negative cut-off point was established to determine compliance. Based on compliance, a WS was determined (from 0 to 100) and farms were classified as 'excellent', 'enhanced', 'acceptable', or 'non-acceptable'. A linear regression model was used to evaluate the association between variables at the herd-level with the WS. Results. Overall, median WS was 82 (min=67; max=97). Two farms were classified as 'excellent' (WS=94.5), 20 as 'enhanced' (WS=82.5), and 3 as 'acceptable' (WS=68). Among all variables, the noncompliance regarding subclinical mastitis, hind-legs and udder dirtiness score, ventral line wounds, Haematobia irritans fly counts, and condition/cleanliness of pre-milking holding area was significantly associated with a 5.6, 3.6, 6.7, 5.1, 6.5, and 6.1 points reduction in the WS, respectively (R2=0.84). Conclusions. Welfare level found across farms was good and encouraging. Nonetheless, six variables were identified as the main contributors to an unfavorable result. Strategies for improvement could focus on these variables to be able to reach a welfare level of ‘excellence’.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49572051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
For some years now, the editorial space of the Journal MVZ Cordoba has been oriented towards addressing human and animal public health issues under the “One Health” concept. We have valued and analyzed scientific articles, issued opinions, sparked debates, proposed lines of research and we have also predicted and warned of the arrival of new diseases both in the country and on the continent
{"title":"Coronavirus: Crónica de una zoonosis anunciada","authors":"Salim Máttar, Marco González Tous","doi":"10.21897/rmvz.2048","DOIUrl":"https://doi.org/10.21897/rmvz.2048","url":null,"abstract":"For some years now, the editorial space of the Journal MVZ Cordoba has been oriented towards addressing human and animal public health issues under the “One Health” concept. We have valued and analyzed scientific articles, issued opinions, sparked debates, proposed lines of research and we have also predicted and warned of the arrival of new diseases both in the country and on the continent","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48956472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Schogor, Patrícia Glombowsky, Fabiana Both, B. Danieli, Fernanda Rigon, J. Reis, Aleksandro Schafer da Silva
Objective. The aims of this study were to assess whether colostrum quality is modified by genetic, physiological and management characteristics in the pre-partum period, as well as evaluate whether quality and composition of colostrum is altered in the freezing process. Material and methods. In the experiment I, colostrum and blood samples of 35 cows (18 Holstein and 17 Jerseys) were collected. In the experiment II, six colostrum samples of Holstein cows were collected and frozen during 60 days. Results. The mean immunoglobulin (Ig) concentration was 77.65 mg/ml to Jersey and 82.77 mg/ml to Holstein. The genetic, parturition order, and the interaction between these factors were no significant on IgG concentration in the colostrum. Also, it was observed an effect genetic of cow in the weight on calf at birth and on three days of age (p<0.0001). Regarding transmission of calf passive immunity, no effects of cow breed and calving order were observed on plasma protein concentration of calf, as well as after three days of freezing. Calves of Holstein (83%) and Jersey (82%) breed showed total serum protein levels above 5.5 g/dL. Holstein cows housed in individual paddocks with diet supplementation provided better quality of colostrum (93.57 mg Ig/mL). Over time, the percentage of fat reduced at freezing, that reduced over time (p<0.05) in Experiment II. Conclusions. The pre-partum management exerts influence on colostrum quality, and the freezing not interfere on centesimal and immunological quality of colostrum, with exception the fat, that decrease along the time.
{"title":"Quality of bovine colostrum and its relation to genetics, management, physiology and its freezing","authors":"A. Schogor, Patrícia Glombowsky, Fabiana Both, B. Danieli, Fernanda Rigon, J. Reis, Aleksandro Schafer da Silva","doi":"10.21897/rmvz.1465","DOIUrl":"https://doi.org/10.21897/rmvz.1465","url":null,"abstract":"Objective. The aims of this study were to assess whether colostrum quality is modified by genetic, physiological and management characteristics in the pre-partum period, as well as evaluate whether quality and composition of colostrum is altered in the freezing process. Material and methods. In the experiment I, colostrum and blood samples of 35 cows (18 Holstein and 17 Jerseys) were collected. In the experiment II, six colostrum samples of Holstein cows were collected and frozen during 60 days. Results. The mean immunoglobulin (Ig) concentration was 77.65 mg/ml to Jersey and 82.77 mg/ml to Holstein. The genetic, parturition order, and the interaction between these factors were no significant on IgG concentration in the colostrum. Also, it was observed an effect genetic of cow in the weight on calf at birth and on three days of age (p<0.0001). Regarding transmission of calf passive immunity, no effects of cow breed and calving order were observed on plasma protein concentration of calf, as well as after three days of freezing. Calves of Holstein (83%) and Jersey (82%) breed showed total serum protein levels above 5.5 g/dL. Holstein cows housed in individual paddocks with diet supplementation provided better quality of colostrum (93.57 mg Ig/mL). Over time, the percentage of fat reduced at freezing, that reduced over time (p<0.05) in Experiment II. Conclusions. The pre-partum management exerts influence on colostrum quality, and the freezing not interfere on centesimal and immunological quality of colostrum, with exception the fat, that decrease along the time.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44529909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jenniffer Alejandra Castellanos Garzón, Rubén Mercado P, Sebastián Peña F, María Carolina Pustovrh R, Liliana Salazar M
Objetivo. Identificar nematodos de la familia Anisakidae en el pez de consumo Mugil curema. Materiales y metodos. Para este estudio, se recolectaron 16 peces Lisa (M. curema) del puerto de Tumaco, una ciudad en la costa colombiana del Pacifico. La identificacion morfologica de las larvas se realizo mediante taxonomia clasica y se calculo el porcentaje de infestacion de larvas. Para la identificacion molecular, se realizo una PCR multiple con cebadores para las especies Anisakis physeteris, Pseudoterranova decipiens, Anisakis simplex sensu stricto, Contracaecum osculatum, Hysterothylacium aduncum y Anisakis pegreffii. Resultados. La revision taxonomica permitio la identificacion de larvas de tipo II del genero Anisakis y larvas del genero Pseudoterranova. Las larvas se aislaron principalmente del intestino, donde se encontro que el 94% de los peces estaban parasitados por nematodos anisakidos. La PCR multiplex permitio la identificacion de la especie A. physeteris (Larva tipo II) y P. decipiens. Conclusiones. Este estudio en el primer reporte del nematodo Anisakidae en Tumaco, Colombia. Estos resultados proporcionan una justificacion convincente para un estudio adicional sobre la familia Anisakidae en Colombia, como un problema de salud publica.
{"title":"Anisakis physeteris and Pseudoterranova decipiens in the Mugil curema fish caught in Tumaco, Colombia","authors":"Jenniffer Alejandra Castellanos Garzón, Rubén Mercado P, Sebastián Peña F, María Carolina Pustovrh R, Liliana Salazar M","doi":"10.21897/rmvz.1781","DOIUrl":"https://doi.org/10.21897/rmvz.1781","url":null,"abstract":"Objetivo. Identificar nematodos de la familia Anisakidae en el pez de consumo Mugil curema. Materiales y metodos. Para este estudio, se recolectaron 16 peces Lisa (M. curema) del puerto de Tumaco, una ciudad en la costa colombiana del Pacifico. La identificacion morfologica de las larvas se realizo mediante taxonomia clasica y se calculo el porcentaje de infestacion de larvas. Para la identificacion molecular, se realizo una PCR multiple con cebadores para las especies Anisakis physeteris, Pseudoterranova decipiens, Anisakis simplex sensu stricto, Contracaecum osculatum, Hysterothylacium aduncum y Anisakis pegreffii. Resultados. La revision taxonomica permitio la identificacion de larvas de tipo II del genero Anisakis y larvas del genero Pseudoterranova. Las larvas se aislaron principalmente del intestino, donde se encontro que el 94% de los peces estaban parasitados por nematodos anisakidos. La PCR multiplex permitio la identificacion de la especie A. physeteris (Larva tipo II) y P. decipiens. Conclusiones. Este estudio en el primer reporte del nematodo Anisakidae en Tumaco, Colombia. Estos resultados proporcionan una justificacion convincente para un estudio adicional sobre la familia Anisakidae en Colombia, como un problema de salud publica.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44954063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manuel Garcia-Ulloa Gomez, L. C. Villanueva-Fonseca, Pedro Sandoval-Rivera, J. A. Hernández-Sepúlveda, A. Domínguez-Orozco, B. P. Villanueva-Fonseca, M. García-Ulloa
Objective. To detect the presence of presumptive hypnospores of the protozoan Perkinsus sp. in a wild population of the Mexican chocolata clam Megapitaria squalida in the southeastern Gulf of California, using Ray´s fluid thioglycollate medium (RFTM). Material and methods. Thirty specimens with size between 56.17 and 69.04 mm were captured each month, during an annual cycle. Infection prevalence and intensity and water parameters were recorded monthly from September 2012 to September 2013. Results. Perkinsus sp. was detected in tissue samples from the Mexican chocolate clam using the RFTM test by the presence of dark round corpuscles that represent parasite´s hypnospores. Monthly samplings revealed a prevalence of 0-43.33% and an infection intensity ranging from 1 to 4 (no infection = 0 hypnospores/entire preparation, to moderate = 34 hypnospores/entire preparation). Conclusions. Perkinsus sp. is reported for the first time in a wild population of M. squalida in the southesternmost Gulf of California. The results indicate that this protozoan is dispersed intraspecifically and would now, potentially, parasiting a new host in the region.
{"title":"Report of presumptive Perkinsus sp. hypnospores in Megapitaria squalida of the Gulf of California with the thioglycollate staining technique","authors":"Manuel Garcia-Ulloa Gomez, L. C. Villanueva-Fonseca, Pedro Sandoval-Rivera, J. A. Hernández-Sepúlveda, A. Domínguez-Orozco, B. P. Villanueva-Fonseca, M. García-Ulloa","doi":"10.21897/rmvz.1805","DOIUrl":"https://doi.org/10.21897/rmvz.1805","url":null,"abstract":"Objective. To detect the presence of presumptive hypnospores of the protozoan Perkinsus sp. in a wild population of the Mexican chocolata clam Megapitaria squalida in the southeastern Gulf of California, using Ray´s fluid thioglycollate medium (RFTM). Material and methods. Thirty specimens with size between 56.17 and 69.04 mm were captured each month, during an annual cycle. Infection prevalence and intensity and water parameters were recorded monthly from September 2012 to September 2013. Results. Perkinsus sp. was detected in tissue samples from the Mexican chocolate clam using the RFTM test by the presence of dark round corpuscles that represent parasite´s hypnospores. Monthly samplings revealed a prevalence of 0-43.33% and an infection intensity ranging from 1 to 4 (no infection = 0 hypnospores/entire preparation, to moderate = 34 hypnospores/entire preparation). Conclusions. Perkinsus sp. is reported for the first time in a wild population of M. squalida in the southesternmost Gulf of California. The results indicate that this protozoan is dispersed intraspecifically and would now, potentially, parasiting a new host in the region.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45252601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Torres-Castro, Viviana Febles-Solís, Silvia Hernández-Betancourt, Henry Noh-Pech, Erendira Estrella, R. Peláez-Sánchez, Alonso Panti-May, Belén G. Herrera-Flores, Bibiana Reyes-Hernández, Javier Sosa-Escalante
Objective. To report the infection with Leptospira in the kidneys of bats from Campeche and Yucatan, Mexico, through the amplification by PCR of two different 16S RNA ribosomal gene fragments. Materials and methods. Bat captures were made at one site in Campeche and two sites in Yucatan. Euthanasia was applied to the captured bats and a necropsy was performed to collect a renal tissue sample that was used in the total DNA extraction. Two different conventional PCR were performed for the amplification of the 16S RNA ribosomal gene fragments. Some sequences from positive products were obtained and analyzed with bioinformatics tools to identify the infectious species of Leptospira. Results. Sixty-nine bats belonging to four families and eight different species were captured. The family with the greatest diversity was Phyllostomidae, with five species. The most captured species was Artibeus jamaicensis (41, 59.4%). Both PCR showed a global infection frequency of 21.7%. The infected species were A. jamaicensis, Pteronotus parnellii, and Chiroderma villosum. The bioinformatic analysis of the positive products yielded a 99.0% identity for Leptospira noguchii, Leptospira borgpetersenii, and Leptospira santarosai. Conclusions. Some bat species of Yucatan and Campeche, Mexico, are renal carriers of pathogenic Leptospira, therefore participating in the transmission cycle in the region. The frequency of infection found in the renal tissue of the captured bats is higher than the one obtained from other reservoirs captured in Yucatan and Campeche. New species of bats are reported as renal Leptospira carriers in Mexico.
{"title":"Leptospira patógenas en murciélagos de Campeche y Yucatán, México","authors":"M. Torres-Castro, Viviana Febles-Solís, Silvia Hernández-Betancourt, Henry Noh-Pech, Erendira Estrella, R. Peláez-Sánchez, Alonso Panti-May, Belén G. Herrera-Flores, Bibiana Reyes-Hernández, Javier Sosa-Escalante","doi":"10.21897/rmvz.1815","DOIUrl":"https://doi.org/10.21897/rmvz.1815","url":null,"abstract":"Objective. To report the infection with Leptospira in the kidneys of bats from Campeche and Yucatan, Mexico, through the amplification by PCR of two different 16S RNA ribosomal gene fragments. Materials and methods. Bat captures were made at one site in Campeche and two sites in Yucatan. Euthanasia was applied to the captured bats and a necropsy was performed to collect a renal tissue sample that was used in the total DNA extraction. Two different conventional PCR were performed for the amplification of the 16S RNA ribosomal gene fragments. Some sequences from positive products were obtained and analyzed with bioinformatics tools to identify the infectious species of Leptospira. Results. Sixty-nine bats belonging to four families and eight different species were captured. The family with the greatest diversity was Phyllostomidae, with five species. The most captured species was Artibeus jamaicensis (41, 59.4%). Both PCR showed a global infection frequency of 21.7%. The infected species were A. jamaicensis, Pteronotus parnellii, and Chiroderma villosum. The bioinformatic analysis of the positive products yielded a 99.0% identity for Leptospira noguchii, Leptospira borgpetersenii, and Leptospira santarosai. Conclusions. Some bat species of Yucatan and Campeche, Mexico, are renal carriers of pathogenic Leptospira, therefore participating in the transmission cycle in the region. The frequency of infection found in the renal tissue of the captured bats is higher than the one obtained from other reservoirs captured in Yucatan and Campeche. New species of bats are reported as renal Leptospira carriers in Mexico.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49310918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. V. Peçanha, Raquel Batista Junger de Carvalho, C. A. Santos-Sousa, Emanoelli Aparecida Rodrigues dos Santos, P. S. Júnior, M. Abidu-Figueiredo
Objective. The aim of this study was explored the duplicity of renal artery in a specimen of Cerdocyon thous, focusing on the possibilities of clinical-surgical implication of this anatomical variation. Materials and Methods. Were dissected 32 specimens of Cerdocyon thous, obtained from the collections of the Laboratorio de Ensino e Pesquisa em Morfologia dos Animais Domesticos e Selvagens do Departamento de Anatomia Animal e Humana, da Universidade Federal Rural do Rio de Janeiro e Laboratorio de Anatomia Animal da Universidade Federal do Pampa. Results. Were observed a numerical variation in the left renal artery in an adult female cadaver. The left kidney had two renal arteries, one cranial and another caudal. The first renal artery of the left kidney, measuring 2.25 cm in length, originated laterally from the abdominal aorta at the level of the third lumbar vertebra. Moreover, it emanated two pre-hilar branches, one dorsal and one ventral, with the ventral branch supplying also to the adrenal gland. The second renal artery also originated laterally from the abdominal aorta at the level of the third lumbar vertebra and measured 2.36 cm in length. It also emitted two pre-hilar branches, one cranial and another caudal, which emitted the ureteral branch. Conclusions. Numerical variations of the renal arteries should be considered in the execution of surgical, radiological and experimental procedures in order to avoid mistakes made due to lack of knowledge of the possibility these variations both in domestic and wild animals.
客观的本研究的目的是探索Cerdocyon Thou标本中肾动脉的双重性，重点探讨这种解剖变异对临床外科意义的可能性。材料和方法。解剖了32个Cerdocyon Thou标本，这些标本来自阿纳托利亚动物与人类部Ensino e Pesquisa em Morfologia dos Animais Domesticos e Selvagens实验室、里约热内卢联邦农村大学Anatomia动物实验室和潘帕联邦大学。后果在一具成年女性尸体中观察到左肾动脉的数值变化。左肾有两条肾动脉，一条是头动脉，另一条是尾动脉。左肾的第一肾动脉，长2.25厘米，从第三腰椎水平的腹主动脉横向起源。此外，它发出两个肝门前分支，一个背侧和一个腹侧，腹侧分支也供应给肾上腺。第二肾动脉也从第三腰椎水平处的腹主动脉侧向起源，长度为2.36cm。它还发出两个肺门前分支，一个是头部分支，另一个是尾部分支，这两个分支发出输尿管分支。结论。在执行外科、放射学和实验程序时，应考虑肾动脉的数值变化，以避免由于缺乏对家畜和野生动物中这些变化的可能性的了解而出现错误。
{"title":"Double renal artery in Cerdocyon thous","authors":"S. V. Peçanha, Raquel Batista Junger de Carvalho, C. A. Santos-Sousa, Emanoelli Aparecida Rodrigues dos Santos, P. S. Júnior, M. Abidu-Figueiredo","doi":"10.21897/rmvz.1713","DOIUrl":"https://doi.org/10.21897/rmvz.1713","url":null,"abstract":"Objective. The aim of this study was explored the duplicity of renal artery in a specimen of Cerdocyon thous, focusing on the possibilities of clinical-surgical implication of this anatomical variation. Materials and Methods. Were dissected 32 specimens of Cerdocyon thous, obtained from the collections of the Laboratorio de Ensino e Pesquisa em Morfologia dos Animais Domesticos e Selvagens do Departamento de Anatomia Animal e Humana, da Universidade Federal Rural do Rio de Janeiro e Laboratorio de Anatomia Animal da Universidade Federal do Pampa. Results. Were observed a numerical variation in the left renal artery in an adult female cadaver. The left kidney had two renal arteries, one cranial and another caudal. The first renal artery of the left kidney, measuring 2.25 cm in length, originated laterally from the abdominal aorta at the level of the third lumbar vertebra. Moreover, it emanated two pre-hilar branches, one dorsal and one ventral, with the ventral branch supplying also to the adrenal gland. The second renal artery also originated laterally from the abdominal aorta at the level of the third lumbar vertebra and measured 2.36 cm in length. It also emitted two pre-hilar branches, one cranial and another caudal, which emitted the ureteral branch. Conclusions. Numerical variations of the renal arteries should be considered in the execution of surgical, radiological and experimental procedures in order to avoid mistakes made due to lack of knowledge of the possibility these variations both in domestic and wild animals.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44499941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manuel Antonio Lepe López, Ederico Villatoro-Paz, Juan Carlos Valdez Sandoval, L. Rios, M. Diaz Rodríguez, Dennis Sigfried Guerra Centeno
Objective. Identify the natural infection of freshwater snails as an intermediate host of Fasciola hepatica in Guatemala. Materials and methods. Freshwater snails were collected in a high-altitude village (3.000 mamsl) from Huehuetenango department, where fasciolasis is endemic. The identification of the snail species was based on the morphological characteristics observed under the stereoscope using an identification key. The trematode phases were searched externally by visual inspection using a light source, and internally by dissection and visual inspection of the tissues under a stereoscope and microscope. Results. 260 snails were found and identified as Pseudosuccinea columella. Two of them were found naturally infected with larval phases of F. hepatica. Conclusions. This is the first report of P. columella carrying phases of F. hepatica in the highlands of Guatemala. It is necessary to increase the research of the ecology of this parasite in other understudied areas in Guatemala and Central America, due to the latent risk of infection for populations of herbivorous animals and humans.
{"title":"Report of Pseudosuccinea columella, infected with Fasciola hepatica at Sierra de los Cuchumatanes, Guatemala","authors":"Manuel Antonio Lepe López, Ederico Villatoro-Paz, Juan Carlos Valdez Sandoval, L. Rios, M. Diaz Rodríguez, Dennis Sigfried Guerra Centeno","doi":"10.21897/rmvz.1616","DOIUrl":"https://doi.org/10.21897/rmvz.1616","url":null,"abstract":"Objective. Identify the natural infection of freshwater snails as an intermediate host of Fasciola hepatica in Guatemala. Materials and methods. Freshwater snails were collected in a high-altitude village (3.000 mamsl) from Huehuetenango department, where fasciolasis is endemic. The identification of the snail species was based on the morphological characteristics observed under the stereoscope using an identification key. The trematode phases were searched externally by visual inspection using a light source, and internally by dissection and visual inspection of the tissues under a stereoscope and microscope. Results. 260 snails were found and identified as Pseudosuccinea columella. Two of them were found naturally infected with larval phases of F. hepatica. Conclusions. This is the first report of P. columella carrying phases of F. hepatica in the highlands of Guatemala. It is necessary to increase the research of the ecology of this parasite in other understudied areas in Guatemala and Central America, due to the latent risk of infection for populations of herbivorous animals and humans.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46562795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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