O. F. Huertas-Molina, Victor Manuel Guzman-Carazo, Luis Guillermo Palacio-Baena, J. Zambrano-Varón, M. Olivera-Angel
Objective. Determine the prevalence of bovine ketosis based on serum concentrations (mmol/ Lt) of beta-hydroxybutyrate (βHB) and estimate its association with early postpartum diseases in dairy cows. Materials and methods. An epidemiological cross-sectional study was carried out with
{"title":"Concentraciones séricas de Beta-hidroxibutirato y su asociación con enfermedades posparto en bovinos de leche","authors":"O. F. Huertas-Molina, Victor Manuel Guzman-Carazo, Luis Guillermo Palacio-Baena, J. Zambrano-Varón, M. Olivera-Angel","doi":"10.21897/rmvz.1821","DOIUrl":"https://doi.org/10.21897/rmvz.1821","url":null,"abstract":"Objective. Determine the prevalence of bovine ketosis based on serum concentrations (mmol/ Lt) of beta-hydroxybutyrate (βHB) and estimate its association with early postpartum diseases in dairy cows. Materials and methods. An epidemiological cross-sectional study was carried out with","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42322973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Donicer Montes-Vergara, C. Lenis-Valencia, D. Hernández-Herrera
Objetivo. Predecir del peso en canal a la edad de beneficio en conejos Nueva Zelanda a partir de medidas corporales. Materiales y métodos. En 100 machos Nueva Zelanda (NZ) criados de forma comercial de 60±3 días, con ayuno de 12 horas, se tomó el peso vivo (PV) el largo de cuerpo dorsal (LCDV) y ventral (LCVV), perímetro del tórax (PTV), largo de lomo (LLV), ancho de lomo (ALV), ancho de tórax (ATV) ancho de cabeza (ACV) largo de cabeza (LCV), perímetro de muslo (PMV), largo de muslo (LMV), perímetro del brazo (PBV) y largo del brazo (LBV). Los conejos fueron sacrificados, pesadas sus canales calientes (PCC) y se tomaron las mismas medidas antes descritas, en la canal. Se realizó estadística descriptiva y el rendimiento en canal caliente (RCC). Se estimaron los coeficientes de correlación con el PCC. Se estableció una ecuación de regresión del PCC, con el procedimiento “Stepwise Regression”. Resultados. Las variables in vivo fueron más homogéneas que en el animal en canal. El RCC fue 54.96±4.03%. La correlación más alta que se encontró con el PCC fue el PV (r= 0.84; p<0.0001). El PTV, PTC, PBV, PBV y ACV presentaron una correlaciones positivas (p<0.02). La ecuación de regresión que mejor se ajustó fue Yi= 86.5 + 0.52PV - 0.47LCDV - 12.9ACV (R2=0.97; Cp-Mallows= 2.62, p<0.01). Conclusiones. El RCC es similar a otros NZ de edad similar. Las medidas en el animal vivo predicen mejor el PCC. Estos resultados se pueden utilizar en programas de mejoramiento genético animal.
{"title":"Predicción del peso en canal al beneficio en conejos Nueva Zelanda a partir de medidas corporales","authors":"Donicer Montes-Vergara, C. Lenis-Valencia, D. Hernández-Herrera","doi":"10.21897/rmvz.1990","DOIUrl":"https://doi.org/10.21897/rmvz.1990","url":null,"abstract":"Objetivo. Predecir del peso en canal a la edad de beneficio en conejos Nueva Zelanda a partir de medidas corporales. Materiales y métodos. En 100 machos Nueva Zelanda (NZ) criados de forma comercial de 60±3 días, con ayuno de 12 horas, se tomó el peso vivo (PV) el largo de cuerpo dorsal (LCDV) y ventral (LCVV), perímetro del tórax (PTV), largo de lomo (LLV), ancho de lomo (ALV), ancho de tórax (ATV) ancho de cabeza (ACV) largo de cabeza (LCV), perímetro de muslo (PMV), largo de muslo (LMV), perímetro del brazo (PBV) y largo del brazo (LBV). Los conejos fueron sacrificados, pesadas sus canales calientes (PCC) y se tomaron las mismas medidas antes descritas, en la canal. Se realizó estadística descriptiva y el rendimiento en canal caliente (RCC). Se estimaron los coeficientes de correlación con el PCC. Se estableció una ecuación de regresión del PCC, con el procedimiento “Stepwise Regression”. Resultados. Las variables in vivo fueron más homogéneas que en el animal en canal. El RCC fue 54.96±4.03%. La correlación más alta que se encontró con el PCC fue el PV (r= 0.84; p<0.0001). El PTV, PTC, PBV, PBV y ACV presentaron una correlaciones positivas (p<0.02). La ecuación de regresión que mejor se ajustó fue Yi= 86.5 + 0.52PV - 0.47LCDV - 12.9ACV (R2=0.97; Cp-Mallows= 2.62, p<0.01). Conclusiones. El RCC es similar a otros NZ de edad similar. Las medidas en el animal vivo predicen mejor el PCC. Estos resultados se pueden utilizar en programas de mejoramiento genético animal. \u0000 ","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49527671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raúl Andrés Molina-Benavides, C. Bustamante-Zamudio, Adriana Martínez-Arias, José Reinel Uribe-Ceballos, Johan Manuel Redondo-Ortegón
Objetivo. Utilizar los sistemas de información geográfica (SIG) como herramienta complementaria para caracterizar la ganadería bovina realizada en la región de la Orinoquia. Materiales y métodos. A través del uso de tecnologías espaciales se recopiló la información concerniente a la orientación ganadera, fisiografía, cobertura vegetal y catastro de la zona de estudio para su posterior análisis a través del software ACCESS de Microsoft. Resultados. En un alto porcentaje de los predios ganaderos ubicados en los cuatro departamentos de la Orinoquía (Casanare:72.7%, Meta:49.5%, Arauca:42% y Vichada:32%) predominan las coberturas de pastos, herbazales y vegetación secundaria, confirmando la expansión en la frontera agropecuaria que es promovida por la actividad ganadera en el país. Conclusiones. El uso de los SIG, permite realizar una mejor planificación y distribución eficiente de los recursos destinados a mejorar el funcionamiento de los sistemas de producción. Por ejemplo, en zonas donde la matriz de coberturas predominante son los pastizales y herbazales, las estrategias en pro de la sostenibilidad pueden enfocarse en la implementación de sistemas silvopastoriles, contrario a lo que pasaría en zonas donde la matriz de coberturas tenga un alto porcentaje de bosques naturales.
{"title":"Caracterización espacial de la ganadería bovina en la Orinoquia colombiana","authors":"Raúl Andrés Molina-Benavides, C. Bustamante-Zamudio, Adriana Martínez-Arias, José Reinel Uribe-Ceballos, Johan Manuel Redondo-Ortegón","doi":"10.21897/rmvz.1720","DOIUrl":"https://doi.org/10.21897/rmvz.1720","url":null,"abstract":"Objetivo. Utilizar los sistemas de información geográfica (SIG) como herramienta complementaria para caracterizar la ganadería bovina realizada en la región de la Orinoquia. Materiales y métodos. A través del uso de tecnologías espaciales se recopiló la información concerniente a la orientación ganadera, fisiografía, cobertura vegetal y catastro de la zona de estudio para su posterior análisis a través del software ACCESS de Microsoft. Resultados. En un alto porcentaje de los predios ganaderos ubicados en los cuatro departamentos de la Orinoquía (Casanare:72.7%, Meta:49.5%, Arauca:42% y Vichada:32%) predominan las coberturas de pastos, herbazales y vegetación secundaria, confirmando la expansión en la frontera agropecuaria que es promovida por la actividad ganadera en el país. Conclusiones. El uso de los SIG, permite realizar una mejor planificación y distribución eficiente de los recursos destinados a mejorar el funcionamiento de los sistemas de producción. Por ejemplo, en zonas donde la matriz de coberturas predominante son los pastizales y herbazales, las estrategias en pro de la sostenibilidad pueden enfocarse en la implementación de sistemas silvopastoriles, contrario a lo que pasaría en zonas donde la matriz de coberturas tenga un alto porcentaje de bosques naturales.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44412696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Rojas-Risco, P. Montalván-Damián, Juan R. Paredes-Valderrama
Objective. To determine the haematological parameters according to the reproductive status of Peruvian Paso horses in the Province of Lambayeque. Materials and methods. The research was conducted on 60 clinically healthy adult horses (> 4 years), without previous drug treatment. Four groups of 15 horses were formed: gelding, entire male, empty mare and pregnant mare. First, a general clinical examination was performed to confirm their good health. Blood was drawn from
{"title":"Parámetros hematológicos en caballos peruanos de paso en la provincia de Lambayeque","authors":"L. Rojas-Risco, P. Montalván-Damián, Juan R. Paredes-Valderrama","doi":"10.21897/rmvz.1982","DOIUrl":"https://doi.org/10.21897/rmvz.1982","url":null,"abstract":"Objective. To determine the haematological parameters according to the reproductive status of Peruvian Paso horses in the Province of Lambayeque. Materials and methods. The research was conducted on 60 clinically healthy adult horses (> 4 years), without previous drug treatment. Four groups of 15 horses were formed: gelding, entire male, empty mare and pregnant mare. First, a general clinical examination was performed to confirm their good health. Blood was drawn from","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47192209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. D. Vega-Britez, Daisy Pérez-Villalba, N. Lesmo-Duarte, José A. Velázquez-Duarte, A. Leonardo, Marciana Retore, Fernando De Vargas-Junior
Objective. To evaluate the quantitative characteristics of the carcass and chemical composition of the meat of purebred Santa Inês (SI) lambs and crosses with Dorper (DO-SI) or Texel (TX-SI), finished in feedlot or semi-feedlot, through a meta-analysis. Materials and methods. Virtual databases such as Google Scholar, Science Direct and Scielo were used to select scientific articles published from 2000 to 2019.
{"title":"Canal y carne de corderos Santa Inés y cruces con Dorper o Texel: Un meta-análisis","authors":"G. D. Vega-Britez, Daisy Pérez-Villalba, N. Lesmo-Duarte, José A. Velázquez-Duarte, A. Leonardo, Marciana Retore, Fernando De Vargas-Junior","doi":"10.21897/rmvz.1960","DOIUrl":"https://doi.org/10.21897/rmvz.1960","url":null,"abstract":"Objective. To evaluate the quantitative characteristics of the carcass and chemical composition of the meat of purebred Santa Inês (SI) lambs and crosses with Dorper (DO-SI) or Texel (TX-SI), finished in feedlot or semi-feedlot, through a meta-analysis. Materials and methods. Virtual databases such as Google Scholar, Science Direct and Scielo were used to select scientific articles published from 2000 to 2019.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49484052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luís Balarezo-Urresta, J. García-Díaz, Ernesto Noval-Artiles
Objetivo. Determinar la influencia de la condición corporal al parto (CCP) en el reinicio de la actividad ovárica (RAO) posparto de la vaca Holstein en la región andina de Ecuador. Materiales y métodos. Se trabajaron 30 vacas. Se determinaron el momento de aparición del folículo dominante (FD), de ovulación y de actividad luteal; la duración del ciclo estral, el volumen del cuerpo lúteo (CL) y las concentraciones de progesterona (P4) en suero sangíneo. Los estadígrafos descriptivos de las variables del RAO y sus indicadores se compararon según la CCP mediante una prueba de t-Student para muestras independientes. Se evaluó la asociación entre la CCP y el RAO posparto mediante un estudio caso control. Resultados. La duración del ciclo estral fue 23.10 días, el 46.67% de las vacas tuvo ciclos normales y el 53.33% ciclos anormales. El FD, la ovulación y la actividad luteal ocurrieron a los 16.63, 27.76 y 41.38 días posparto, respectivamente; antes (p<0.05) en vacas con CCP ≥ 3.5; en las que fueron mayores (p<0.05) el volumen del CL y las concentraciones de P4. La CCP se correlacionó (p<0.05) con los parámetros del RAO. Las vacas con CCP <3.5 puntos, tienen 10.50 veces más probabilidades de tener RAO tardío que las que tienen CCP ≥ de 3.5 puntos. Conclusiones. El RAO fue temprano, sobre el influyó la CCP, la que constituyó un factor de riesgo (p<0.05) para que las vacas tengan un RAO tardío.
{"title":"Condición corporal y reinicio de la actividad ovárica posparto en vacas Holstein en Ecuador","authors":"Luís Balarezo-Urresta, J. García-Díaz, Ernesto Noval-Artiles","doi":"10.21897/rmvz.1859","DOIUrl":"https://doi.org/10.21897/rmvz.1859","url":null,"abstract":"Objetivo. Determinar la influencia de la condición corporal al parto (CCP) en el reinicio de la actividad ovárica (RAO) posparto de la vaca Holstein en la región andina de Ecuador. Materiales y métodos. Se trabajaron 30 vacas. Se determinaron el momento de aparición del folículo dominante (FD), de ovulación y de actividad luteal; la duración del ciclo estral, el volumen del cuerpo lúteo (CL) y las concentraciones de progesterona (P4) en suero sangíneo. Los estadígrafos descriptivos de las variables del RAO y sus indicadores se compararon según la CCP mediante una prueba de t-Student para muestras independientes. Se evaluó la asociación entre la CCP y el RAO posparto mediante un estudio caso control. Resultados. La duración del ciclo estral fue 23.10 días, el 46.67% de las vacas tuvo ciclos normales y el 53.33% ciclos anormales. El FD, la ovulación y la actividad luteal ocurrieron a los 16.63, 27.76 y 41.38 días posparto, respectivamente; antes (p<0.05) en vacas con CCP ≥ 3.5; en las que fueron mayores (p<0.05) el volumen del CL y las concentraciones de P4. La CCP se correlacionó (p<0.05) con los parámetros del RAO. Las vacas con CCP <3.5 puntos, tienen 10.50 veces más probabilidades de tener RAO tardío que las que tienen CCP ≥ de 3.5 puntos. Conclusiones. El RAO fue temprano, sobre el influyó la CCP, la que constituyó un factor de riesgo (p<0.05) para que las vacas tengan un RAO tardío.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42825353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leticia Cañas-López, Sandra B Hernández-Dávila, Juan Carlos Vázquez-Chagoyán, José Simón Martínez-Castañeda, R. Fajardo-Muñoz, B. Valladares-Carranza, César Ortega S
Objective. To perform the isolation of spring viraemia of carp virus (SVCV) in common carp (Cyprinus carpio) and evaluate its growth in different cell types and viral survival at different temperatures. Materials and methods. Ten carps of between 400-500 grams of a lagoon in central Mexico were processed for diagnosis of SVCV by isolation in cell culture and by RT-PCR. The virus obtained was inoculated into EPC, BF-2, CHSE-214 and RTG-2 cells to determine differences in virus growth; the survival of virus stored at room temperature (TA 20-25 °C), refrigeration (REF 4° C) and freezing (CONG -80 °C) up to eleven months was also evaluated. Internal organ samples were processed for histological analysis. Results. The fish analyzed did not show external signs suggestive of disease but internally and histopathologically lesions suggestive of systemic infection were observed. SVCV was isolated in EPC and BF-2 cells, and confirmed by semi-nested RT-PCR. SVCV only induced CPE in EPC and BF-2 cells, and was negative in RTG-2 and CHSE-214. The virus conserved at TA lost viability after four months post-infection (mpi), being total at six mpi; while REF and CONG were stable during the eleven months. Conclusions. Subclinical SVCV infection was confirmed in carp that presented histological lesions associated with this infection; SVCV only caused CPE in EPC and BF-2 cells; and the virus kept in refrigeration and at -80 °C retained its viability up to eleven months; while TA was lost in six months.
{"title":"Características de replicación y supervivencia del virus de viremia primaveral de la carpa (SVCV) aislado en México","authors":"Leticia Cañas-López, Sandra B Hernández-Dávila, Juan Carlos Vázquez-Chagoyán, José Simón Martínez-Castañeda, R. Fajardo-Muñoz, B. Valladares-Carranza, César Ortega S","doi":"10.21897/rmvz.1875","DOIUrl":"https://doi.org/10.21897/rmvz.1875","url":null,"abstract":"Objective. To perform the isolation of spring viraemia of carp virus (SVCV) in common carp (Cyprinus carpio) and evaluate its growth in different cell types and viral survival at different temperatures. Materials and methods. Ten carps of between 400-500 grams of a lagoon in central Mexico were processed for diagnosis of SVCV by isolation in cell culture and by RT-PCR. The virus obtained was inoculated into EPC, BF-2, CHSE-214 and RTG-2 cells to determine differences in virus growth; the survival of virus stored at room temperature (TA 20-25 °C), refrigeration (REF 4° C) and freezing (CONG -80 °C) up to eleven months was also evaluated. Internal organ samples were processed for histological analysis. Results. The fish analyzed did not show external signs suggestive of disease but internally and histopathologically lesions suggestive of systemic infection were observed. SVCV was isolated in EPC and BF-2 cells, and confirmed by semi-nested RT-PCR. SVCV only induced CPE in EPC and BF-2 cells, and was negative in RTG-2 and CHSE-214. The virus conserved at TA lost viability after four months post-infection (mpi), being total at six mpi; while REF and CONG were stable during the eleven months. Conclusions. Subclinical SVCV infection was confirmed in carp that presented histological lesions associated with this infection; SVCV only caused CPE in EPC and BF-2 cells; and the virus kept in refrigeration and at -80 °C retained its viability up to eleven months; while TA was lost in six months.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48083919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Reyes-Bossa, Hernan Isaias Medina-Rios, J. Cardona-Álvarez
Objective. To determine the ultrasonographic morphometric parameters of the palmar metacarpal tendons and ligaments of clinically healthy Colombian creole horses. Materials and methods. Thirty clinically healthy Colombian creole horses of 6.4 ± 2.5 years were evaluated in different areas of the department of Córdoba (Colombia). The variables cross-sectional area (cm2), medial lateral width (ALM) (cm) and dorsal palmar thickness (EDP) (cm) in the structures of the metacarpal region in the two anterior members were measured by means of ultrasound. Results. Ultrasonography revealed
{"title":"Evaluación de medidas morfométricas de tendones y ligamentos metacarpales palmares por ultrasonografía en caballos criollos colombianos","authors":"B. Reyes-Bossa, Hernan Isaias Medina-Rios, J. Cardona-Álvarez","doi":"10.21897/rmvz.1863","DOIUrl":"https://doi.org/10.21897/rmvz.1863","url":null,"abstract":"Objective. To determine the ultrasonographic morphometric parameters of the palmar metacarpal tendons and ligaments of clinically healthy Colombian creole horses. Materials and methods. Thirty clinically healthy Colombian creole horses of 6.4 ± 2.5 years were evaluated in different areas of the department of Córdoba (Colombia). The variables cross-sectional area (cm2), medial lateral width (ALM) (cm) and dorsal palmar thickness (EDP) (cm) in the structures of the metacarpal region in the two anterior members were measured by means of ultrasound. Results. Ultrasonography revealed","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48833258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María De los Ángeles Largo Quintero, Jhon Jairo Tuberquia Londoño, Mauricio Sánchez Vallejo, Mariana Machado Arango, Santiago Rodríguez, Santiago Monsalve, N. M. Correa-Valencia, Jorge Arturo Fernández Silva
Objective. To determine the presence of anti-Mycobacterium avium subsp. paratuberculosis (MAP) antibodies in white-tailed deer (Odocoileus virginianus) and red brocket deer (Mazama rufina), captured in the Orinoquia and Caribbean regions of Colombia. Materials and methods. A total of 44 blood serum samples from deer of species M. rufina and O. virginianus were collected under field conditions between 2014 and 2016. An ELISA commercial kit was used to detect anti-MAP antibodies. An animal was considered ELISA-positive at a sample-to-positive ratio (S/P%) of ≥ 0.4, as recommended by the manufacturer. Results. The 50% (22/44) of the animals were positive, corresponding to 10 females and 12 males. An 81.8% (18/22) and 77.3% (17/22) of these seropositive animals were captured in the Orinoquia region and were reported as adults, respectively. Conclusions. It is not known how or when MAP was introduced in the Colombian deer population in the study regions. The most plausible hypothesis to explain the presence of antibodies against MAP in these wild populations is transmission by contact with infected bovine cattle since, in both regions, these species share pastures. This is the first study to explore MAP infection in wild animals in Colombia. These findings support the need for further studies using different direct diagnostic techniques and research approaches that allow the definition of links in the infection dynamics between wild and domestic mammals in Colombia.
{"title":"Detección de anticuerpos anti-Mycobacterium avium subsp. paratuberculosis (MAP) en venados silvestres en Colombia","authors":"María De los Ángeles Largo Quintero, Jhon Jairo Tuberquia Londoño, Mauricio Sánchez Vallejo, Mariana Machado Arango, Santiago Rodríguez, Santiago Monsalve, N. M. Correa-Valencia, Jorge Arturo Fernández Silva","doi":"10.21897/rmvz.1809","DOIUrl":"https://doi.org/10.21897/rmvz.1809","url":null,"abstract":"Objective. To determine the presence of anti-Mycobacterium avium subsp. paratuberculosis (MAP) antibodies in white-tailed deer (Odocoileus virginianus) and red brocket deer (Mazama rufina), captured in the Orinoquia and Caribbean regions of Colombia. Materials and methods. A total of 44 blood serum samples from deer of species M. rufina and O. virginianus were collected under field conditions between 2014 and 2016. An ELISA commercial kit was used to detect anti-MAP antibodies. An animal was considered ELISA-positive at a sample-to-positive ratio (S/P%) of ≥ 0.4, as recommended by the manufacturer. Results. The 50% (22/44) of the animals were positive, corresponding to 10 females and 12 males. An 81.8% (18/22) and 77.3% (17/22) of these seropositive animals were captured in the Orinoquia region and were reported as adults, respectively. Conclusions. It is not known how or when MAP was introduced in the Colombian deer population in the study regions. The most plausible hypothesis to explain the presence of antibodies against MAP in these wild populations is transmission by contact with infected bovine cattle since, in both regions, these species share pastures. This is the first study to explore MAP infection in wild animals in Colombia. These findings support the need for further studies using different direct diagnostic techniques and research approaches that allow the definition of links in the infection dynamics between wild and domestic mammals in Colombia.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47808439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Correa-Valencia, Ferney Arango-Lezcano, J. Fernández-Silva
Objective. To report the frequency of seropositivity against Mycobacterium avium subsp. paratuberculosis (MAP) and to explore the factors associated with the positive outcome, both at herd and animal level. Materials and methods. A cross-sectional study was carried out on 204 dairy cows, result of the crossing of different breeds, from five low-tropic dairy herds, located in three municipalities of the Provinces of Sucre and Cordoba (Colombia) in 2018. The animals were randomly selected, and blood samples were collected from each one. A commercial ELISA kit was used to analyze the sera, and the association between these variables and the result to ELISA (p<0.05) was explored. Results. The 17.2% (35/204; 95% CI: 12.0-22.3%) of the cows were positive for MAP by the ELISA test, and the five herds had seropositive animals. The herd-level variable presence of other ruminants in co-grazing with cattle in the last 2 years and the animal-level variables age and parity were associated with the positive ELISA results. Conclusions. The present study found that 17.2% of the cows and 100% of the herds were MAP positive by the ELISA test. Additionally, associated variables were identified that may be of interest to both producers and veterinarians and guide their approach to disease management.
{"title":"Mycobacterium avium subsp. paratuberculosis antibodies in cows of low-tropic dairy herds in Colombia","authors":"N. Correa-Valencia, Ferney Arango-Lezcano, J. Fernández-Silva","doi":"10.21897/rmvz.1782","DOIUrl":"https://doi.org/10.21897/rmvz.1782","url":null,"abstract":"Objective. To report the frequency of seropositivity against Mycobacterium avium subsp. paratuberculosis (MAP) and to explore the factors associated with the positive outcome, both at herd and animal level. Materials and methods. A cross-sectional study was carried out on 204 dairy cows, result of the crossing of different breeds, from five low-tropic dairy herds, located in three municipalities of the Provinces of Sucre and Cordoba (Colombia) in 2018. The animals were randomly selected, and blood samples were collected from each one. A commercial ELISA kit was used to analyze the sera, and the association between these variables and the result to ELISA (p<0.05) was explored. Results. The 17.2% (35/204; 95% CI: 12.0-22.3%) of the cows were positive for MAP by the ELISA test, and the five herds had seropositive animals. The herd-level variable presence of other ruminants in co-grazing with cattle in the last 2 years and the animal-level variables age and parity were associated with the positive ELISA results. Conclusions. The present study found that 17.2% of the cows and 100% of the herds were MAP positive by the ELISA test. Additionally, associated variables were identified that may be of interest to both producers and veterinarians and guide their approach to disease management.","PeriodicalId":49598,"journal":{"name":"Revista Mvz Cordoba","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48648003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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