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Diversidad genética de ovinos criollos colombianos 哥伦比亚克里奥尔羊的遗传多样性
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-09-18 DOI: 10.21897/RMVZ.2185
Nini Vivas A, V. Landi, Jaime Eduardo Muñoz Florez, Moris De Jesús Bustamante Yánez, Luz Álvarez Franco
Ojective. genetical characterization the creole Colombian sheep and their relationships with breeds of European origins. Materials and methods. Blood samples of 261 sheeps from the following populations were collected: Criollos de Lana (CL), Mora Colombiana (MC), Criollo de Pelo (CP), criollos mestizos (Mes), Hampshire (Hamp), Corriedale (Corr), Katahdin (Kath), Pelibuey (Pel), in 40 farms of 8 departments (Cordoba, Magdalena, Cesar, Atlantico, Valle del Cauca, Narino, Boyaca, Tolima) and 30 samples of Merino Spanish, Merino Precoz (MP), Merinofleischschaf (MF), Segureno (Seg) and Uda (UD) de Nigeria. A total of 15 microsatellites markers were included inthis study. Results. In creole sheep, the average number of alleles was found 6.20±1.48 (CL), 7.27±1.39 (CP) y 3.60±1.55 (MC); high genetic diversity found (high heterozygosities 75%), negative values in the FIS revealed high degree of introgression; furthermore the FST revealed genetic structure in both: Creole sheep (FST=0.02**) and departments (FST=0.039**).According to genetic distance, the creole Colombian sheep differs with outsider sheep. Conclusions. The results recommend protecting the Creole sheep production because it has been threatened by constant cross with foreign breeds, which would lead to the loss of genetic identity and adapting traits of creole sheep.themselves.
Ojective。哥伦比亚克里奥尔羊的遗传特征及其与欧洲起源品种的关系。材料和方法。在8个省(科尔多瓦、马格达莱纳、塞萨尔、Atlantico、cauka山谷、Narino、Boyaca、Tolima)的40个农场采集了以下种群261只羊的血样:Criollos de Lana (CL)、Mora Colombiana (MC)、Criollo de Pelo (CP)、Criollos mestizos (Mes)、Hampshire (Hamp)、Corriedale (Corr)、Katahdin (Kath)、Pelibuey (Pel),以及30只西班牙美利奴、美利奴Precoz (MP)、Merinofleischschaf (MF)、Segureno (Seg)和Uda (UD) de Nigeria。本研究共纳入15个微卫星标记。结果。克里奥尔羊平均等位基因数分别为6.20±1.48 (CL)、7.27±1.39 (CP)和3.60±1.55 (MC);遗传多样性高(杂合度高75%),FIS值为负值表明遗传渗入程度高;此外,FST还揭示了克里奥尔羊(FST=0.02**)和科(FST=0.039**)的遗传结构。根据遗传距离,克里奥尔哥伦比亚羊与外来羊不同。结论。研究结果建议保护克里奥尔羊的生产,因为它一直受到与外国品种杂交的威胁,这将导致克里奥尔羊自身遗传特性和适应性特征的丧失。
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引用次数: 11
Canine mammary gland tumors: risk factors and their epidemiological influence in Manizales-Colombia 犬乳腺肿瘤:危险因素及其在哥伦比亚马尼萨莱斯的流行病学影响
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.21897/rmvz.1888
A. Clavijo-Maldonado, J. M. Pérez-Zapata, Ê. Ferreira, C. Vargas-Hernández, F. A. Rivera-Páez
Objective. To describe the prevalence of canine mammary gland tumors (CMT) in females in the municipality of Manizales-Colombia from 2014-2017. Materials and methods. A database of 15961 patients was consolidated. The variables analyzed were, breed, age, reproductive history, weight, diet type and clinical characterization of the tumor with the TNM (tumor-node-metastases) staging system. The statistical analysis include Pearson’s chi-squared test with Yates correction for continuity and Fisher’s exact test (p<0.05), relative risk and odds ratio (CI95%) and Kaplan-Meier estimator for survival analysis. Results. The incidence in purebred dogs was 79.14%, with a peak at the average age of 9.3 years old. Pearson’s chi-squared test and the relative risk and odds ratios indicated a high risk for purebreds (p=0.019, 3.96/100, 1.64, respectively). Females of ages between 9 and 12 years old showed a 74% likelihood of developing a mammary tumor. No found significant relation to weight or reproductive stage but indeed a high association with homemade diet (p<0.001). The inguinal mammary pairs were the most affected (6.9%). The Kaplan-Meier estimate showed a higher survival of surgically-intervened patients, with 2013 days of survival after diagnosis with surgery compared to 1484 days without surgery. Conclusions. The study confirmed the relevance of risk factors, breed type, age, body condition and diet type in the mammary tumor presentation. Furthermore, it highlights the need for improving and integrating the veterinary diagnostic information systems, considering their importance in public health.
目标。描述2014-2017年哥伦比亚马尼萨莱斯市女性犬乳腺肿瘤(CMT)的患病率。材料和方法。合并了15961例患者的数据库。分析的变量包括肿瘤品种、年龄、生殖史、体重、饮食类型和临床特征,肿瘤-淋巴结-转移分期系统。统计学分析采用连续性的Pearson卡方检验和Fisher精确检验(p<0.05),相对风险和优势比(CI95%),生存分析采用Kaplan-Meier估计。结果。纯种犬发病率为79.14%,平均年龄9.3岁为高峰。Pearson卡方检验、相对危险度和比值比均显示纯种犬患病风险较高(p分别为0.019、3.96/100、1.64)。年龄在9到12岁之间的女性患乳腺肿瘤的可能性为74%。没有发现体重或生育阶段有显著关系,但与自制饮食有高度关联(p<0.001)。腹股沟乳对受影响最大(6.9%)。Kaplan-Meier估计显示,手术干预患者的生存率更高,手术诊断后的生存时间为2013天,而未手术的生存时间为1484天。结论。该研究证实了危险因素、品种类型、年龄、身体状况和饮食类型与乳腺肿瘤表现的相关性。此外,考虑到兽医诊断信息系统在公共卫生中的重要性,它强调了改进和整合兽医诊断信息系统的必要性。
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引用次数: 0
Análisis bibliométrico de los artículos científicos publicados en Medicina Veterinaria y Zootecnia en Colombia 2010-2019 2010-2019年哥伦比亚兽医和畜牧业科学论文的文献计量分析
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.21897/rmvz.2114
Marco González T, Luis Carlos Salgado Arroyo
Objective. Analyze the scientific production in Veterinary Medicine and Animal Husbandry in Colombia in the period 2010-2019. Materials and methods. A retrospective cross-sectional descriptive study was carried out, through the bibliometric analysis of articles published in nine journals in the area of Veterinary Medicine and Animal Husbandry in Colombia. The information was obtained by searching the
客观的分析2010-2019年期间哥伦比亚兽医和畜牧业的科学生产情况。材料和方法。通过对哥伦比亚兽医和畜牧业领域九种期刊上发表的文章进行文献计量分析,进行了一项回顾性横断面描述性研究。该信息是通过搜索
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引用次数: 1
Use of secnidazole and homeopathy for giardiasis control in dogs 塞克硝唑和顺势疗法在犬贾第鞭毛虫病控制中的应用
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.21897/rmvz.2004
Patrícia Glombowsky, Gabriela Campigotto, Maiara Sulzbach-Marchiori, Jorge A. Favaretto, Gabriela Miotto-Galli, A. S. Da silva
Dogs with Giardia infections have diarrhea that persists for days, even after treatment. We managed canine giardiasis using two distinct approaches: treatment with the antiprotozoan secnidazole alone; and secnidazole plus a homeopathic product that is indicated for control of diarrhea in dogs. Treatment I: Ten beagle dogs showed clinical signs of severe diarrhea. Their feces revealed large numbers of cysts consistent with Giardia sp. Therefore, we treated the dogs with secnidazole (10 mg/kg, single dose) given orally. Five days after treatment, all dogs were negative for Giardia spp. Treatment II: Three months later, the same ten Beagles again presented with diarrhea. Feces were collected, revealing Giardia sp. in all dogs. This time, we treated them with an antiprotozoan (secnidazole, 10 mg/kg dose) combined with a commercial homeopathic drug for diarrhea control (3 g dose/animal at 12 h interval). We found that three to five homeopathic doses were needed to achieve normal fecal scores in all dogs. After 5 days of treatment, stool samples had normal consistency and were negative for Giardia. This is the first record of using secnidazole to control canine giardiasis associated with homeopathic drugs to prevent diarrhea.
感染贾第鞭毛虫的狗腹泻持续数天,甚至在治疗后也是如此。我们使用两种不同的方法治疗犬贾第虫病:单独使用抗原生动物塞克硝唑治疗;和塞克硝唑加一种顺势疗法产品,用于控制狗腹泻。治疗一:10只比格犬出现严重腹泻的临床症状。它们的粪便显示出大量与贾第鞭毛虫一致的囊肿。因此,我们用塞克硝唑(10 mg/kg,单剂量)口服治疗这些狗。治疗五天后,所有狗的贾第鞭毛虫均为阴性。治疗二:三个月后,同样的十只比格犬再次出现腹泻。收集粪便,发现所有狗都有贾第鞭毛虫。这一次,我们用抗原生动物(塞克硝唑,10 mg/kg剂量)与商业顺势疗法药物联合治疗腹泻(3 g剂量/只,间隔12小时)。我们发现,需要三到五个顺势疗法剂量才能使所有狗的粪便得分正常。治疗5天后,粪便样本具有正常稠度,贾第鞭毛虫呈阴性。这是首次使用塞克硝唑控制犬贾第鞭毛虫病,并使用顺势疗法药物预防腹泻。
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引用次数: 0
Detección de anticuerpos contra Neospora caninum en caninos del área urbana y rural de Cumaral, Meta-Colombia Meta-Colombia Cumaral城市和农村地区犬中抗犬新孢子虫抗体的检测
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-08-31 DOI: 10.21897/rmvz.1879
Paula Sofia Méndez-Ramírez, Julián Marín-Henao, Agustín Góngora-Orjuela, J. L. Parra-Arango, D. Piedrahita, Jenny J. Chaparro-Gutiérrez
Objective. To estimate the seroprevalence to Neospora caninum in canines of the urban and rural area of Cumaral, Meta and determine some risk factors associated with seropositivity. Materials and methods. A cross-sectional study was carried out in 222 dogs (112 dogs from the urban area and 110 dogs from the rural area), the sample size was calculated by using Epidat v program. 3.1. The sera were analyzed using the Indirect Immunofluorescence technique for IgG with a commercial kit. Frequency analyzes by chi-square of independence were performed in SPSS v. 25.0 Results. The general seroprevalence was 36.9% (95% CI: 30.9-43.5%). The seropositivity between the groups
客观的为了估计Cumaral、Meta城市和农村地区犬科动物中犬新孢子虫的血清流行率,并确定与血清阳性相关的一些风险因素。材料和方法。对222只狗(112只来自城市地区,110只来自农村地区)进行了横断面研究,使用Epidat v程序计算样本量。3.1使用商业试剂盒使用IgG的间接免疫荧光技术分析血清。在SPSS v.25.0中使用独立性卡方进行频率分析。结果。总血清阳性率为36.9%(95%可信区间:30.9-43.5%)
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引用次数: 0
intraluminal Uso intraluminal de stent de nitinol Dual para colapso completo de tráquea en un maltés: reporte de caso 腔内双镍钛诺支架在腔内应用于马耳他完全气管塌陷:病例报告
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-08-24 DOI: 10.21897/rmvz.1923
Rodrigo Jose De Lavalle-Galvis, José Pinzón D, Ariel Mendoza D, Amanda Ramos R, J. Guzmán, T. Oviedo S, Mario de la Puente
Anamnesis: paciente canino, maltés, femenino, castrado, 4 años y 5 Kg. de peso El motivo principal de consulta fue tos y dificultad respiratoria grave. Hallazgos clínicos y de laboratorio: Durante la evaluación clínica, el paciente presentó cianosis, estridor inspiratorio y espiratorio severo, tos en graznido especialmente después de una emoción. Se realizó un examen a través de una broncoscopia, que reveló una disminución dinámica en el diámetro de la luz traqueal, un colapso severo en las porciones cervicales y torácicas. Enfoque de tratamiento: Debido a la gravedad del colapso traqueal, se decidió la implantación de un stent de nitinol. La elección del stent es fundamental; Fue necesario realizar una medición del diámetro y la longitud de la tráquea para minimizar los efectos secundarios y los rechazos. La tráquea presentó variabilidad del diámetro a nivel torácico por esta razón se decidió utilizar un stent dual, el stent se colocó en observación directa utilizando un broncoscopio pediátrico. Conclusión: Presentamos un caso en el que se usó un stent dual, indicado en casos en que el diámetro de la tráquea varía, obteniendo excelentes resultados ya que se ajustó a los diferentes diámetros traqueales que el paciente presentó en los niveles cervical y torácico.
回忆:犬患者,马耳他,女性,阉割,4岁,5公斤。我们进行了一项回顾性研究,以确定患者是否患有慢性阻塞性肺病(copd)。临床和实验室结果:在临床评估中,患者表现为发绀、严重吸气和呼气喘振,特别是情绪后咳嗽。通过支气管镜检查进行检查,发现气管光直径动态减小,颈部和胸部严重塌陷。治疗方法:由于气管塌陷的严重程度,决定植入镍钛诺支架。支架的选择至关重要;有必要测量气管的直径和长度,以减少副作用和排斥反应。在胸腔水平上,气管直径变化较大,因此决定采用双支架,采用小儿支气管镜直接观察。结论:我们提出了一个使用双支架的病例,适用于气管直径不同的病例,由于它适应了患者在颈和胸水平的不同气管直径,取得了良好的效果。
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引用次数: 0
Caracterización morfoestructural e índices zoométricos de hembras Ovino de Pelo Criollo Colombiano “OPC” Sudán 哥伦比亚克里奥尔毛“OPC”苏丹母羊的形态结构特征和动物指标
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-08-22 DOI: 10.21897/rmvz.1379
J. M. Flórez-Murillo, M. D. J. Hernández-Pérez, Moris Bustamante-Yánez, Oscar Vergara-Garay
Objective. The objective of this study was to describe the body measurements and zoometric indices of Sudán Bayo and Sudán Blanco, two breed varieties of Colombian creole woolless sheep (OPC). Materials and methods. This research was carried out in the departments of Córdoba, Cesar, and
目标。研究了哥伦比亚克里奥尔无毛羊(OPC)的两个品种Sudán Bayo和Sudán Blanco的体型测量和动物计量指标。材料和方法。这项研究是在Córdoba, Cesar和
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引用次数: 6
Frecuencia de helmintos gastrointestinales en roedores sinantrópicos capturados en el Zoológico de Barranquilla 巴兰基利亚动物园共人啮齿动物胃肠道蠕虫的频率
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-08-20 DOI: 10.21897/rmvz.1944
Johan Romero-Herrera, Lina Henao-Montoya, Henrique Guimarães-Riva, J. E. Jaimes-Dueñez
Objective. The aim of this work was to characterize and describe the frequency of the gastrointestinal parasites in synanthropic rodents captured at the Barranquilla Zoo. Materials and methods. The rodents were captured using Tomahawk traps with the help of non-toxic bait and subsequently
目标。这项工作的目的是表征和描述在巴兰基亚动物园捕获的合群啮齿动物胃肠道寄生虫的频率。材料和方法。这些啮齿动物是用战斧式陷阱在无毒诱饵的帮助下捕获的
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引用次数: 0
Consumo de fragmentos proteicos y digestibilidad de proteína en dietas suministradas a bovinos Chino Santandereano 中国Santandereano牛饲粮中蛋白质碎片采食量和蛋白质消化率的研究
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-08-17 DOI: 10.21897/rmvz.1876
David Esteban Contreras-Marquez, Juan Sebastian Quintero-Pardo, Edwin Davier Correa-Rojas, Emiro Rafael Canchila-Asencio, Heidy Yeritza Gutiérrez-Zocadagui
Objective. To quantify the concentration and consumption of the protein fragments (NPN, TP, RDTP, SDTP, NDIP, and ADIP) present in the diet supplied to cattle, and its effect on the digestibility of crude protein. Materials and methods. Diets offered to Chino Santandereano cattle in stables were analyzed, receiving different levels of supplementation, using four animals in the 4x4 Latin square outline, the treatments being: NS, not supplemented; LOW, supplemented with an amount relative to 0.5% of body weight; MEDIUM, supplemented with an amount relative to 1.0% of body weight; HIGH, supplemented with a quantity relative to 1.5% of body weight. Consumption was determined daily, and digestibility through total stool collection in the last two days of each period. Results. A higher concentration of crude protein was found in supplement compared to grass (p<0.001), the forage exhibiting a higher concentration of CPNPN (p<0.001), at the same time, that the supplement presented higher concentration of TP (p<0.001) and RDTP (p=0.027). Supplemented animals presented higher consumption of CP, CPNPN (p=0.037), TP, NDIP, RDTP, ADIP and SDTP (p<0.05), however, when the concentration that represents the consumption of ADIP in the consumption of CP was determined, no difference was observed between supplemented and NS (p=0.078). Higher digestibility of CP was found in supplemented animals when contrasted with NS (p<0.001), an upward linear effect was observed between supplemented treatments as the level of supplementation increased. Conclusions. Supplementation improves the digestibility of crude protein by providing a greater amount of highly digestible nitrogenous fragments.
目标。定量测定饲粮中蛋白质片段(NPN、TP、RDTP、SDTP、NDIP和ADIP)的浓度和消耗量及其对牛粗蛋白质消化率的影响。材料和方法。采用4头4 × 4拉丁方框牛,对饲养在马厩中的Chino Santandereano牛日粮进行分析,添加不同水平的饲粮,处理为:NS,不添加;低,补充量相对于体重的0.5%;中,添加量相对于体重的1.0%;高,补充量相对于体重的1.5%。每天测定食用量,每期最后两天通过收集粪便总量测定消化率。结果。饲粮中粗蛋白质含量高于草料(p<0.001), CPNPN含量高于草料(p<0.001), TP含量高于草料(p<0.001), RDTP含量高于草料(p=0.027)。饲粮添加组动物对CP、CPNPN、TP、NDIP、RDTP、ADIP和SDTP的消耗均较高(p=0.037),但测定其在CP消耗中代表ADIP消耗的浓度时,饲粮添加组与饲粮添加组之间无显著差异(p=0.078)。饲粮中添加粗蛋白质的动物消化率高于饲粮中添加粗蛋白质的动物(p<0.001),随着饲粮添加水平的增加,饲粮中添加粗蛋白质的动物消化率呈线性上升趋势。结论。通过提供更多高可消化的含氮片段,提高了粗蛋白质的消化率。
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引用次数: 0
Tipificación molecular de Mycobacterium avium subsp. paratuberculosis en lecherías de Antioquia, Colombia 禽分枝杆菌亚种的分子分型。哥伦比亚安蒂奥基亚奶牛场的副结核
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2020-08-13 DOI: 10.21897/rmvz.1816
N. M. Correa-Valencia, Roberto Damián Moyano, M. Romano, J. A. Fernández-Silva
Objective. To determine Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in environmental samples from Colombian dairy herds. Materials and methods. Environmental samples from 25 IS900-qPCR MAP-positive dairy herds were cultured by duplicate in Herrold ́s egg yolk medium with mycobactin J to obtain isolates. Suspicious colonies were confirmed by MAPIS900-qPCR. Positive DNA was sub-typed using mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) and multilocus short sequence repeats (MLSSR) techniques to analyze the genetic differences between the isolates. Results. Sub-typing revealed two different genotypes by MIRU-VNTR (INMV 2 and INMV 36). MLSSR technique was carried out to increase the
目标。目的:测定禽分枝杆菌亚种。哥伦比亚奶牛群环境样本中的副结核(MAP)分子多样性。材料和方法。选取25头IS900-qPCR map阳性奶牛的环境样品,在含有mycoblin J的Herrold’s蛋黄培养基中重复培养,获得分离菌株。可疑菌落经MAPIS900-qPCR确认。采用分枝杆菌穿插重复单位-可变数目串联重复(MIRU-VNTR)和多位点短序列重复(MLSSR)技术对阳性DNA进行分型,分析分离株间的遗传差异。结果。MIRU-VNTR分型显示两种不同的基因型(INMV 2和INMV 36)。采用MLSSR技术,提高了玉米的抗氧化能力
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引用次数: 3
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Revista Mvz Cordoba
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