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Synthesis and evaluation of in vitro antioxidant, anticancer, and antibacterial properties of new benzylideneiminophenylthiazole analogues 新型苯亚氨基苯基噻唑类似物的合成及其体外抗氧化、抗癌和抗菌性能评估
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-17 DOI: 10.1186/s13065-024-01273-5
Ali Khoshbakht, Jafar Abbasi Shiran, Mansour Miran, Saghi Sepehri

A series of new benzylideneiminophenylthiazole analogues were designed and synthesized. Common spectroscopic methods, such as FT-IR, 1H-, 13C-NMR, and MASS spectra, and elemental analysis, were used to confirm the molecular structures. Then, the antioxidant, cytotoxicity, and anti-bacterial effects of synthesized analogues were assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), three cancer cell lines, and two bacterial strains, respectively. Among the analogues, 7f was detected as the most potent compound for antioxidant activity. Moreover, the compounds 7b, 7f, and 7 g exhibited the maximum cytotoxicity activity against MCF-7, HepG-2, and A549 cell lines, respectively. Finally, 7e showed the highest anti-bacterial activity against both S. aureus and E. coli strains. It was concluded from the antioxidant, cytotoxicity, and anti-bacterial effects that the benzylideneiminophenylthiazoles might serve as candidate molecules for the development of small molecules with medicinal potential.

设计并合成了一系列新的苯亚氨基苯基噻唑类似物。利用常见的光谱方法,如傅立叶变换红外光谱、1H-、13C-NMR 和 MASS 光谱以及元素分析,确认了这些化合物的分子结构。然后,分别针对 2,2-二苯基-1-苦基肼(DPPH)、三种癌细胞株和两种细菌菌株评估了合成的类似物的抗氧化性、细胞毒性和抗菌效果。在这些类似物中,7f 是抗氧化活性最强的化合物。此外,化合物 7b、7f 和 7 g 分别对 MCF-7、HepG-2 和 A549 细胞株表现出最大的细胞毒性活性。最后,7e 对金黄色葡萄球菌和大肠杆菌的抗菌活性最高。从抗氧化、细胞毒性和抗菌效果中得出的结论是,苯亚氨基苯基噻唑可作为候选分子,用于开发具有药用潜力的小分子化合物。
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引用次数: 0
Rapid UV-Vis spectrophotometric method aided by firefly-PLS models for the simultaneous quantification of ciprofloxacin, lomefloxacin, and enrofloxacin in their laboratory mixture, dosage forms and water samples: greenness and blueness assessment 萤火虫-PLS模型辅助紫外可见分光光度法同时定量实验室混合物、剂型和水样中的环丙沙星、洛美沙星和恩诺沙星:绿度和蓝度评估
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-16 DOI: 10.1186/s13065-024-01286-0
Ali Alqahtani, Taha Alqahtani, Adel Al Fatease, Enas H. Tolba

Herein, a novel UV spectrophotometric method coupled with chemometric tools was developed for the simultaneous determination of three fluoroquinolone antibiotics: ciprofloxacin, lomefloxacin, and enrofloxacin. Such integration of UV spectroscopy and chemometric analysis proved to be a simple, rapid, and cost-effective approach for the quantification of these clinically important pharmaceutical compounds and aid in their quality control analysis. The method employed firefly algorithm for variable selection and partial least squares (PLS) regression for model calibration. The developed method was validated by independent test set in addition the accuracy, intra and inter-day precision as per ICH guidelines which showed a satisfactory performance with mean recovery ranged between 98.18 and 101.83 with %RSD < 2. Besides, the developed method displayed ultrasensitive levels with LODs (0.0803, 0.1125, 0.1309 µg/mL) and LOQs (0.2434, 0.3409, 0.3968 µg/mL) for ciprofloxacin, lomefloxacin, and enrofloxacin, respectively. The greenness and blueness of the developed method were also evaluated using the recently proposed Analytical GREEnness metric approach (AGREE) and Blue applicability grade index (BAGI) tools, which showed a high AGREE score of 0.79 and a BAGI score of 77.5. These results indicate that the developed method provides an environmentally friendly alternative to the traditionally used chromatographic techniques, while maintaining high analytical practicability. Finally, the application of the developed methodology was demonstrated on real pharmaceutical and tap water samples, and the results were in good agreement with those obtained by the reference HPLC method indicating the reliability and suitability of the proposed spectrophotometric method for routine analysis of fluoroquinolone antibiotics.

本文开发了一种新型紫外分光光度法,并结合化学计量学工具,用于同时测定三种氟喹诺酮类抗生素:环丙沙星、洛美沙星和恩诺沙星。事实证明,这种紫外光谱与化学计量分析相结合的方法是一种简单、快速、经济高效的方法,可用于定量检测这些临床上重要的药物化合物,并有助于对其进行质量控制分析。该方法采用萤火虫算法进行变量选择,并采用偏最小二乘法(PLS)回归进行模型校准。根据 ICH 指南,该方法在准确度、日内和日间精密度方面的表现令人满意,平均回收率在 98.18 和 101.83 之间,%RSD 为 2。此外,所开发的方法对环丙沙星、洛美沙星和恩诺沙星具有超灵敏度,其最低检出限(0.0803、0.1125、0.1309 µg/mL)和最低定量限(0.2434、0.3409、0.3968 µg/mL)分别为 0.0803、0.1125、0.1309 µg/mL、0.3968 µg/mL。此外,还使用最近提出的 "分析绿色度量方法(AGREE)"和 "蓝色适用性等级指数(BAGI)"工具对所开发方法的 "绿色度 "和 "蓝色度 "进行了评价,结果表明该方法的 "绿色度 "和 "蓝色度 "分别高达0.79分和77.5分。这些结果表明,所开发的方法是传统色谱技术的环保型替代方法,同时保持了较高的分析实用性。最后,在真实的药品和自来水样品上演示了所开发方法的应用,结果与参考的 HPLC 方法一致,表明所建议的分光光度法在氟喹诺酮类抗生素常规分析中的可靠性和适用性。
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引用次数: 0
Correction to: Synthesis of novel carbazole hydrazine‑carbothioamide scaffold as potent antioxidant, anticancer and antimicrobial agents 更正为合成新型咔唑肼硫代甲酰胺支架作为强效抗氧化剂、抗癌剂和抗菌剂
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-16 DOI: 10.1186/s13065-024-01274-4
İrfan Çapan, Mohammed Hawash, Mohammed T. Qaoud, Levent Gülüm, Ezgi Nurdan Yenilmez Tunoglu, Kezban Uçar Çifci, Bekir Sıtkı Çevrimli, Yusuf Sert, Süleyman Servi, İrfan Koca, Yusuf Tutar
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引用次数: 0
Oxindole–benzothiazole hybrids as CDK2 inhibitors and anticancer agents: design, synthesis and biological evaluation 作为 CDK2 抑制剂和抗癌剂的吲哚-苯并噻唑混合物:设计、合成和生物学评价
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-13 DOI: 10.1186/s13065-024-01277-1
Heba T. Abdel-Mohsen

In the current study, molecular hybridization between the oxindole core and benzothiazole system through an acetohydrazide moiety was accomplished for the design of a new series of oxindole–benzothiazole hybrids 9ar targeting CDK2 for cancer therapy. The afforded hybrids displayed promising growth inhibitory activity on NCI cancer cell lines at 10 µM. Compound 9o displayed mean GI% = 55.91%. Based on the potent activity of 9o, it was further assessed for its cytotoxic activity at five dose level and it demonstrated GI50 reaching 2.02 µM. Analysis of the cell cycle of the prostate cancer cell line DU145 after treatment with 9o confirmed its ability to arrest its cell cycle at the G1 phase. Moreover, 9o proved its ability to potentiate the apoptosis and necrosis of the same cell line. Furthermore, the oxindole–benzothiazole hybrids 9b, 9f and 9o showed IC50 = 0.70, 0.20 and 0.21 µM, respectively on CDK2. Besides, molecular docking simulation of the synthesized oxindole–benzothiazole hybrid 9o proved the expected binding mode which involves the accommodation of the oxindole moiety in the ATP binding pocket where it is involved in hydrogen bonding and hydrophobic interactions with the essential amino acids in the hinge region while the benzothiazole moiety is oriented toward the solvent region. Investigation of the physicochemical properties of the hybrids 9ar highlights their acceptable ADME properties that can be somewhat developed for the discovery of new anticancer agents.

在目前的研究中,通过乙酰肼分子实现了吲哚核心与苯并噻唑系统之间的分子杂交,从而设计出一系列新的吲哚-苯并噻唑杂交化合物 9a-r,用于靶向 CDK2 治疗癌症。所得到的杂交化合物在 10 µM 的浓度下对 NCI 癌细胞株具有良好的生长抑制活性。化合物 9o 的平均 GI% = 55.91%。基于 9o 的强效活性,研究人员进一步评估了它在五个剂量水平上的细胞毒性活性,结果表明它的 GI50 达到 2.02 µM。前列腺癌细胞株 DU145 经 9o 处理后的细胞周期分析表明,9o 能使细胞周期停滞在 G1 期。此外,9o 还能促进同一细胞株的细胞凋亡和坏死。此外,吲哚-苯并噻唑混合物 9b、9f 和 9o 对 CDK2 的 IC50 分别为 0.70、0.20 和 0.21 µM。此外,对合成的吲哚-苯并噻唑混合物 9o 进行的分子对接模拟证明了预期的结合模式,即吲哚分子位于 ATP 结合袋中,与铰链区的必需氨基酸发生氢键和疏水相互作用,而苯并噻唑分子则朝向溶剂区。对杂交化合物 9a-r 的理化性质进行的研究表明,它们具有可接受的 ADME 特性,可用于开发新的抗癌药物。
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引用次数: 0
Corrosion potential and theoretical studies of fabricated Schiff base for carbidic austempered ductile iron in 1M H2SO4 solution 在 1M H2SO4 溶液中对碳化奥氏体球墨铸铁的希夫碱进行腐蚀电位和理论研究
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-13 DOI: 10.1186/s13065-024-01278-0
Ghalia A. Gaber, Lamiaa Z. Mohamed, Hayam A. Aly, Shimaa Hosny

In this body of work, a chemical known as 2-cyano-N-(4-morpholino benzyl dine) acetohydrazide (CMBAH) is explored for its ability to suppress the carbidic austempered ductile iron (CADI) corrosion in 1M H2SO4. Density functional theory was used in experiments and theoretical investigations to investigate the inhibiting impact. The corrosion of CADI alloys in 1M H2SO4 produced a corrosion resistance superior to that of CADI heat treatment (H.T.). As-cast carbidic ductile iron (CDI) 4 alloy with 1.5%t Cr-Nb has a corrosion rate (C.R.) of 11.69 mm/year, which drops to 5.31 mm/year at HT-275 °C and 6.13 mm/year at HT-375 °C. When describing the adsorption of inhibitors, the Langmuir adsorption isotherm is the most effective method. The findings of the Gads show that the inhibition was induced mainly by the physisorption on the surface CADI alloys. In addition to this, it was found that the results of the experiments and the hypotheses were largely harmonious with one another. The formation of protective layers on the CADI surfaces is also visible in the images captured by the SEM. In 1M H2SO4, these Schiff base inhibitors effectively prevent corrosion caused by CADI. However, the combination of inhibitors leads to a fine microstructure with ausferrite and narrow ferrite needles, promoting corrosion resistance. The CADI needles rated an upper ausferritic microstructure with wide ferrite needles.

在这项研究中,我们探索了一种名为 2-氰基-N-(4-吗啉基苄基丁)乙酰甲酰肼(CMBAH)的化学物质在 1M H2SO4 中抑制碳化物奥氏体回火球墨铸铁(CADI)腐蚀的能力。在实验和理论研究中使用了密度泛函理论来研究其抑制作用。CADI 合金在 1M H2SO4 中的耐腐蚀性优于 CADI 热处理(H.T.)。含 1.5%t Cr-Nb 的铸造碳化物球墨铸铁 (CDI) 4 合金的腐蚀速率 (C.R.) 为 11.69 毫米/年,在 HT-275 °C 时降至 5.31 毫米/年,在 HT-375 °C 时降至 6.13 毫米/年。在描述抑制剂的吸附时,朗缪尔吸附等温线是最有效的方法。Gads 的研究结果表明,抑制作用主要是由 CADI 合金表面的物理吸附引起的。除此之外,还发现实验结果和假设在很大程度上是相互协调的。在扫描电子显微镜拍摄的图像中也可以看到 CADI 表面保护层的形成。在 1M H2SO4 中,这些希夫碱抑制剂能有效防止 CADI 引起的腐蚀。但是,抑制剂的组合会产生具有奥氏体和窄铁素体针的精细微观结构,从而提高耐腐蚀性。CADI 针评定出的是具有宽铁素体针的上奥氏体微观结构。
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引用次数: 0
A sustainable HPLC method coupled with diode array detection for versatile quantification of telmisartan, chlorthalidone and amlodipine in a fixed-dose antihypertensive formulation and dissolution studies 可持续高效液相色谱法与二极管阵列检测相结合,用于固定剂量降压制剂中替米沙坦、氯沙坦和氨氯地平的多功能定量分析和溶出研究
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-12 DOI: 10.1186/s13065-024-01276-2
Mona A. Kamel, Christine K. Nessim, Adel M. Michael, Samah S. Abbas, Hoda M. Marzouk

Cardiovascular diseases, especially hypertension, stand as prominent contributors to global mortality. Hypertension, often referred to as a silent killer syndrome, necessitates the use of multiple medications for effective control and management. A new environmentally friendly HPLC–DAD method is introduced in this study for the concurrent analysis of telmisartan (TEL), chlorthalidone (CHT) and amlodipine besylate (AML), in both pure forms and combined pharmaceutical dosage form. An isocratic elution mode was employed to achieve chromatographic separation, utilizing an Inertsil C18 column (250 × 4.6 mm, 5.0 µm) and a mobile phase mixture of acetonitrile and phosphate buffer (pH 3.0 ± 0.1) with ratio of 35:65, v/v. The separation was achieved within 10 min at a flow rate of 1.0 mL/min. The proposed method's validation was carried out following the guidelines outlined by the International Council for Harmonisation (ICH). The achieved linearity range was 1.0–140.0 μg/mL for TEL and 1.0–100.0 μg/mL for CHT and AML with quantification limits of 0.061, 0.177, and 0.313 μg/mL for TEL, CHT, and AML, respectively. The fixed combination tablet dosage form demonstrated acceptable release profile, as indicated by the in-vitro dissolution studies. The studied dissolution media were phosphate buffer pH 7.5, 0.01 N HCl, and water, utilizing a USP type II apparatus at 37 ± 0.5 °C with a stirring rate of 75 rpm. The proposed method was applied successfully for the quality assessment of Telma-ACT® Tablets with good precision and accuracy. Various tools were used for evaluating the level of greenness, including Green Analytical Procedure Index (GAPI), Analytical Greenness Metric for Sample Preparation (AGREEprep), Analytical Eco-Scale (AES), and Analytical Method Greenness Score (AMGS). These tools had confirmed the eco-friendliness of the proposed method. Additionally, the newly introduced White Analytical Chemistry (WAC), and the Blue Applicability Grade Index (BAGI) have been specifically developed to evaluate the sustainability and the applicability of the method.

心血管疾病,尤其是高血压,是造成全球死亡的主要原因。高血压通常被称为 "无声杀手综合征",需要使用多种药物进行有效控制和管理。本研究介绍了一种新型环保型 HPLC-DAD 方法,用于同时分析纯品和复方药物剂型中的替米沙坦(TEL)、氯沙坦(CHT)和苯磺酸氨氯地平(AML)。色谱分离采用等度洗脱模式,使用 Inertsil C18 色谱柱(250 × 4.6 mm,5.0 µm)和乙腈与磷酸盐缓冲液(pH 3.0 ± 0.1)混合流动相(体积比为 35:65)。流速为 1.0 mL/min,10 分钟内完成分离。根据国际协调理事会(ICH)的指导方针,对所建议的方法进行了验证。TEL的线性范围为1.0-140.0 μg/mL,CHT和AML的线性范围为1.0-100.0 μg/mL,定量限分别为0.061、0.177和0.313 μg/mL。体外溶出度研究表明,固定复方片剂具有可接受的释放曲线。所研究的溶出介质为 pH 值为 7.5 的磷酸盐缓冲液、0.01 N HCl 和水,使用 USP II 型仪器,温度为 37 ± 0.5 °C,搅拌速率为 75 rpm。所提议的方法成功地应用于 Telma-ACT® 片剂的质量评估,具有良好的精确度和准确性。该方法使用了多种工具来评估绿色程度,包括绿色分析程序指数(GAPI)、样品制备分析绿色度量(AGREEprep)、分析生态尺度(AES)和分析方法绿色度评分(AMGS)。这些工具证实了拟议方法的生态友好性。此外,还专门开发了新引入的白色分析化学(WAC)和蓝色适用性等级指数(BAGI),以评估该方法的可持续性和适用性。
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引用次数: 0
Solubility and thermodynamic analysis of aceclofenac in different {Carbitol + water} mixtures at various temperatures 不同温度下醋氯芬酸在不同{卡必醇+水}混合物中的溶解度和热力学分析
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-12 DOI: 10.1186/s13065-024-01287-z
Faiyaz Shakeel, Ramadan Al-Shdefat, Mohammad A. Altamimi, Usama Ahmad

The solubility and thermodynamic properties of the anti-inflammatory drug aceclofenace (ACF) have been assessed in a range of {2-(2-ethoxyethoxy)ethanol (Carbitol) + water} combinations at temperatures ranging from 298.2 K to 318.2 K and atmospheric pressure of 101.1 kPa. The shake flask method was employed to determine the solubility of ACF, and various models including “van’t Hoff, Apelblat, Buchowski-Ksiazczak λh, Yalkowsky-Roseman, Jouyban-Acree, and Jouyban-Acree-van’t Hoff models” were used to validate the results. The computational models demonstrated a strong correlation with the experimental ACF solubility data, as indicated by the error values of < 3.0%. In the compositions of {Carbitol + water}, the ACF mole fraction solubility was enhanced by temperature and Carbitol mass fraction. The solubility of ACF in mole fraction was found to be lowest in pure water (1.07 × 10− 6 at 298.2 K), and highest in pure Carbitol (1.04 × 10− 1 at 318.2 K). Based on the positive values of the calculated thermodynamic parameters, the dissolution of ACF was determined to be “endothermic and entropy-driven” in all of the {Carbitol + water} solutions that were studied. It was also observed that enthalpy controls the solvation of ACF in solutions containing {Carbitol + water}. ACF-Carbitol had the strongest molecular interactions in contrast to ACF-water. Based on the results of this study, Carbitol holds significant potential for enhancing the solubility of ACF in water.

在 298.2 K 至 318.2 K 的温度和 101.1 kPa 的大气压力下,对消炎药醋氯芬ace(ACF)在一系列{2-(2-乙氧基乙氧基)乙醇(卡比妥)+水}组合中的溶解度和热力学性质进行了评估。采用振荡烧瓶法测定了 ACF 的溶解度,并使用 "van't Hoff、Apelblat、Buchowski-Ksiazczak λh、Yalkowsky-Roseman、Jouyban-Acree 和 Jouyban-Acree-van't Hoff 模型 "等多种模型对结果进行了验证。计算模型与 ACF 溶解度实验数据具有很强的相关性,误差值为 3.0%。在{卡必醇+水}成分中,ACF 分子分数溶解度随温度和卡必醇质量分数的变化而增强。在纯水中,ACF 的摩尔分数溶解度最低(298.2 K 时为 1.07 × 10- 6),而在纯卡比妥中最高(318.2 K 时为 1.04 × 10- 1)。根据计算得出的热力学参数的正值,可以确定在研究的所有{卡必醇+水}溶液中,ACF 的溶解都是 "内热和熵驱动 "的。研究还发现,焓控制着 ACF 在含有{卡比醇 + 水}的溶液中的溶解。与 ACF-水相比,ACF-卡必醇的分子相互作用最强。根据这项研究的结果,卡必醇在提高 ACF 在水中的溶解度方面具有很大的潜力。
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引用次数: 0
Exploring the role of topological descriptors to predict physicochemical properties of anti-HIV drugs by using supervised machine learning algorithms 利用监督机器学习算法探索拓扑描述符在预测抗艾滋病毒药物理化性质方面的作用
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-12 DOI: 10.1186/s13065-024-01266-4
Wakeel Ahmed, Shahid Zaman, Eizzah Asif, Kashif Ali, Emad E. Mahmoud, Mamo Abebe Asheboss

In order to explore the role of topological indices for predicting physio-chemical properties of anti-HIV drugs, this research uses python program-based algorithms to compute topological indices as well as machine learning algorithms. Degree-based topological indices are calculated using Python algorithm, providing important information about the structural behavior of drugs that are essential to their anti-HIV effectiveness. Furthermore, machine learning algorithms analyze the physio-chemical properties that correspond to anti-HIV activities, making use of their ability to identify complex trends in large, convoluted datasets. In addition to improving our comprehension of the links between molecular structure and effectiveness, the collaboration between machine learning and QSPR research further highlights the potential of computational approaches in drug discovery. This work reveals the mechanisms underlying anti-HIV effectiveness, which paves the way for the development of more potent anti-HIV drugs. This work reveals the mechanisms underlying anti-HIV efficiency, which paves the way for the development of more potent anti-HIV drugs which demonstrates the invaluable advantages of machine learning in assessing drug properties by clarifying the biological processes underlying anti-HIV behavior, which paves the way for the design and development of more effective anti-HIV drugs.

为了探索拓扑指数在预测抗 HIV 药物理化性质方面的作用,本研究使用了基于 python 程序的算法来计算拓扑指数以及机器学习算法。使用 Python 算法计算基于度的拓扑指数,可提供药物结构行为的重要信息,这些信息对药物的抗 HIV 效力至关重要。此外,机器学习算法还能分析与抗艾滋病毒活性相对应的物理化学特性,从而利用其在大型复杂数据集中识别复杂趋势的能力。除了提高我们对分子结构和有效性之间联系的理解,机器学习和 QSPR 研究之间的合作还进一步凸显了计算方法在药物发现方面的潜力。这项工作揭示了抗艾滋病病毒有效性的内在机制,为开发更有效的抗艾滋病病毒药物铺平了道路。这项工作揭示了抗 HIV 有效性的内在机制,为开发更有效的抗 HIV 药物铺平了道路。通过阐明抗 HIV 行为的生物过程,这项工作展示了机器学习在评估药物特性方面的宝贵优势,为设计和开发更有效的抗 HIV 药物铺平了道路。
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引用次数: 0
Spectrofluorimetric determination of tapinarof via Zn (II) complexation and assessment of its topical dosage application 通过锌(II)络合测定他匹那罗夫的光谱荧光测定法及其外用剂量评估
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-10 DOI: 10.1186/s13065-024-01271-7
Hesham Salem, Mahmoud A. Abdelmajed, Hoda Madian, Nadeen Emad, Sara Osama, Amir Ata, Ebtihal Samir

Topical tapinarof is used to treat plaque psoriasis (a skin disease in which red and scaly patches form are appeared on some areas of the body). The goal of the current research is to establish a facile and rapid fluorimetric technique for tapinarof analysis. The approach relied on the reaction between the drug and zinc ion through metal complexation to produce a highly-fluorescent product. The fluorescence was further enhanced by adding sodium dodecyl sulfate, and it was observed at 542 nm following excitation at 497 nm. With a correlation coefficient of 0.9997, the association between emission intensity and tapinarof concentration was linear between 2.0 and 120 ng mL−1. 1.021 ng mL−1 was the quantitation limit while 0.366 ng mL−1 was the detection limit. The buffer type, pH and concentration, type of surfactant and concentration, and finally the diluting solvent were among the reaction conditions that were closely examined and it was found that the optimum conditions were obtained upon employing teorell-stenhagen buffer optimized at pH 6.0, 1.38 × 10–2 M SDS and distilled water as a solvent are the suitable choice. With great precision and reliability, the drug under study was quantified using this method in ointment formulations. The proposed method's level of greenness was assessed using two methodologies: the analytical greenness metric (AGREE) and the Green Analytical Procedure Index (GAPI), with good recovery results ensuring high efficiency of the proposed approach on analysis of ointment without any interference from additives and excipients.

外用他匹那罗福可用于治疗斑块状银屑病(一种在身体某些部位出现红色鳞屑斑块的皮肤病)。目前研究的目标是建立一种简便、快速的荧光分析技术来分析他匹那罗福。该方法依赖于药物和锌离子通过金属络合反应生成高荧光产物。加入十二烷基硫酸钠后,荧光进一步增强,在 497 nm 处激发后,在 542 nm 处观察到荧光。相关系数为 0.9997,发射强度与 tapinarof 浓度在 2.0 至 120 纳克 mL-1 之间呈线性关系。1.021 ng mL-1 为定量限,0.366 ng mL-1 为检测限。通过对缓冲液的种类、pH 值和浓度、表面活性剂的种类和浓度以及稀释溶剂等反应条件的仔细研究,发现采用 pH 值为 6.0 的 teorell-stenhagen 缓冲液、1.38 × 10-2 M SDS 和蒸馏水作为溶剂是最佳的选择。使用该方法对软膏配方中的药物进行了定量分析,结果精确可靠。采用分析绿色度量(AGREE)和绿色分析程序指数(GAPI)这两种方法评估了所建议方法的绿色程度,结果表明回收率良好,确保了所建议方法在分析软膏时的高效率,且不受任何添加剂和辅料的干扰。
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引用次数: 0
Investigation of green synchronous spectrofluorimetric approach for facile sensitive estimation of two co-administered anti-cancer drugs; curcumin and doxorubicin in their laboratory-prepared mixtures, human plasma, and urine 研究绿色同步光谱荧光法,以方便灵敏地估算两种联合用药的抗癌药物;姜黄素和多柔比星在实验室制备的混合物、人体血浆和尿液中的含量
IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2024-09-09 DOI: 10.1186/s13065-024-01272-6
Diaa Dagher, Heba Elmansi, Jenny Jeehan Nasr, Nahed El-Enany

Recently, phytochemicals play an important role in cancer management. Curcumin (CUR), a natural phytochemical, has been co-administered with widespread chemotherapeutic agents such as doxorubicin (DOX) due to its excellent antitumor activity and the ability to lower the adverse reactions and drug resistance cells associated with DOX use. The present study aims to determine DOX and CUR utilizing a label-free, selective, sensitive, and precise synchronous spectrofluorimetric method. The obvious overlap between the emission spectra of DOX and CUR prevents simultaneous estimation of both analytes by conventional spectrofluorimetry. To solve such a problem, synchronous spectrofluorimetric measurements were recorded at Δλ = 20 nm, utilizing ethanol as a diluting solvent. Curcumin was recorded at 442.5 nm, whereas DOX was estimated at 571.5 nm, each at the zero-crossing point of the other one. The developed method exhibited linearity over a concentration range of 0.04–0.40 μg/mL for CUR and 0.05–0.50 μg/mL for DOX, respectively. The values of limit of detection (LOD) were 0.009 and 0.012 µg/mL, while the values of limit of quantitation (LOQ) were 0.028 and 0.037 µg/mL for CUR and DOX, respectively. The adopted approach was carefully validated according to the guidelines of ICH Q2R1. The method was utilized to estimate CUR and DOX in laboratory-prepared mixtures and human biological matrices. It showed a high percentage of recoveries with minimal RSD values. Additionally, three different tools were utilized to evaluate the greenness of the proposed approach.

最近,植物化学物质在癌症治疗中发挥了重要作用。姜黄素(CUR)是一种天然植物化学物质,由于其卓越的抗肿瘤活性以及降低与 DOX 使用相关的不良反应和耐药细胞的能力,已被广泛用于多柔比星(DOX)等化疗药物。本研究旨在利用一种无标记、选择性、灵敏和精确的同步光谱荧光法测定 DOX 和 CUR。由于 DOX 和 CUR 的发射光谱存在明显重叠,传统的光谱荧光测定法无法同时测定这两种分析物。为了解决这个问题,我们利用乙醇作为稀释溶剂,在 Δλ = 20 nm 处记录了同步光谱荧光测定。姜黄素在 442.5 纳米波长处被记录,而 DOX 则在 571.5 纳米波长处被估算,每一个波长处都是另一个波长的零交叉点。所开发的方法在姜黄素和 DOX 的浓度范围分别为 0.04-0.40 μg/mL 和 0.05-0.50 μg/mL 时呈线性关系。CUR和DOX的检出限(LOD)分别为0.009和0.012微克/毫升,定量限(LOQ)分别为0.028和0.037微克/毫升。所采用的方法根据ICH Q2R1指南进行了仔细的验证。该方法用于测定实验室制备的混合物和人体生物基质中的CUR和DOX。该方法的回收率高,RSD 值最小。此外,还使用了三种不同的工具来评估拟议方法的绿色性。
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BMC Chemistry
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