Pub Date : 2023-09-20DOI: 10.18705/2782-3806-2023-3-4-6-12
T. V. Vavilova, O. V. Sirotkina
The system of personnel training for work in modern clinical diagnostic laboratories should meet the challenges that the development of personalized medicine sets for the medical community. Traditionally, departments and courses of clinical laboratory diagnostics in medical higher educational institutions as part of the training of highly qualified personnel — residency and postgraduate studies, carry out such work. The clinical orientation of laboratory diagnostics and its special role in providing personalized medicine urgently requires the close integration with clinical departments, which is implemented in the Almazov National Medical Research Centre within the framework of the University Clinic.
{"title":"Training of specialists to provide laboratory support in the field of personalized medicine at the Almazov Centre","authors":"T. V. Vavilova, O. V. Sirotkina","doi":"10.18705/2782-3806-2023-3-4-6-12","DOIUrl":"https://doi.org/10.18705/2782-3806-2023-3-4-6-12","url":null,"abstract":"The system of personnel training for work in modern clinical diagnostic laboratories should meet the challenges that the development of personalized medicine sets for the medical community. Traditionally, departments and courses of clinical laboratory diagnostics in medical higher educational institutions as part of the training of highly qualified personnel — residency and postgraduate studies, carry out such work. The clinical orientation of laboratory diagnostics and its special role in providing personalized medicine urgently requires the close integration with clinical departments, which is implemented in the Almazov National Medical Research Centre within the framework of the University Clinic.","PeriodicalId":499018,"journal":{"name":"Russian Journal for Personalized Medicine","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136377799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-20DOI: 10.18705/2782-3806-2023-3-4-13-19
E. I. Dyachenko, L. V. Bel’skaya
Background . The relevance of studying changes in the activity of metabolic enzymes in saliva in lymphomas lies in the non-invasive method of research, the search for new ways in the diagnosis of oncology to detect the disease in the early stages, as well as to obtain a complete picture of the course of the pathological process. The activity of certain biochemical enzymes is significantly higher in saliva than in blood serum. In addition, saliva is a less hazardous environment for laboratory testing than blood, which reduces the risk of infection for medical personnel. Objective . The aim of this work was to search for biochemical markers in saliva in Hodgkin’s and non-Hodgkin’s lymphomas, the value of which was statistically significantly different from the values of the same markers among healthy subjects included in the control group . Design and methods . In the case-control study, volunteers were divided into 2 groups: the main group, with a histologically confirmed diagnosis of Hodgkin’s lymphoma (53 people) or non-Hodgkin’s lymphoma (82 people) and the control group (135 people), apparently healthy individuals. Inclusion in groups occurred in parallel. All participants underwent a biochemical study of saliva to determine the content of amine nitrogen, the activity of enzymes (AlAT, AsAT, GGT, alkaline phosphatase). The patients of the main group were recruited on the basis of the Clinical Oncological Dispensary (Omsk, Russian Federation). Results . A significant increase in amino nitrogen (p < 0,0084), ALT (p < 0,0205), AST (p < 0,0047), GGT (p<0,0291) was found in the group of patients diagnosed with non-Hodgkin’s lymphoma, compared with subjects from the control group. Conclusion . A preliminary hypothesis was put forward that aminotransferases such as ALT, AST and GGT can be used not only as indicators of liver damage. A change in the activity of amine transferases with a simultaneous increase in the activity of amino nitrogen may reflect a pronounced redistribution of nitrogen in the body for the synthesis of new non-essential amino acids that are necessary for neoplastic cells as structural components for their growth and proliferation. This work also confirms that saliva is an informative diagnostic liquid that can be used in the routine practice of a clinician.
背景。研究淋巴瘤中唾液代谢酶活性变化的相关性在于采用无创的研究方法，寻找肿瘤诊断的新途径，在早期发现疾病，全面了解病理过程的过程。唾液中某些生化酶的活性明显高于血清。此外，与血液相比，唾液对实验室检测的危害更小，这降低了医务人员感染的风险。目标。本研究的目的是寻找霍奇金淋巴瘤和非霍奇金淋巴瘤患者唾液中的生化标记物，这些标记物的值与对照组健康受试者的相同标记物的值有统计学差异。设计和方法。在病例对照研究中，志愿者被分为两组:主组，经组织学确诊为霍奇金淋巴瘤(53人)或非霍奇金淋巴瘤(82人);对照组(135人)，明显健康的个体。被纳入群体是并行发生的。所有参与者都进行了唾液的生化研究，以确定胺氮的含量，酶(AlAT, AsAT, GGT，碱性磷酸酶)的活性。主要组的患者是在临床肿瘤药房(鄂木斯克，俄罗斯联邦)的基础上招募的。结果。氨基氮显著增加(p <000084)， p <[p <]与对照组相比，非霍奇金淋巴瘤患者组中发现GGT (p<0,0291)。结论。初步提出ALT、AST、GGT等转氨酶不仅可以作为肝损害的指标。胺转移酶活性的变化与氨基氮活性的同时增加可能反映了体内氮的明显重新分配，以合成新的非必需氨基酸，而非必需氨基酸是肿瘤细胞生长和增殖所必需的结构成分。这项工作也证实了唾液是一种信息丰富的诊断液体，可以用于临床医生的日常实践。
{"title":"Changes in the content of aminonitrogen and the activity of aminotransferases in saliva in lymphomas","authors":"E. I. Dyachenko, L. V. Bel’skaya","doi":"10.18705/2782-3806-2023-3-4-13-19","DOIUrl":"https://doi.org/10.18705/2782-3806-2023-3-4-13-19","url":null,"abstract":"Background . The relevance of studying changes in the activity of metabolic enzymes in saliva in lymphomas lies in the non-invasive method of research, the search for new ways in the diagnosis of oncology to detect the disease in the early stages, as well as to obtain a complete picture of the course of the pathological process. The activity of certain biochemical enzymes is significantly higher in saliva than in blood serum. In addition, saliva is a less hazardous environment for laboratory testing than blood, which reduces the risk of infection for medical personnel. Objective . The aim of this work was to search for biochemical markers in saliva in Hodgkin’s and non-Hodgkin’s lymphomas, the value of which was statistically significantly different from the values of the same markers among healthy subjects included in the control group . Design and methods . In the case-control study, volunteers were divided into 2 groups: the main group, with a histologically confirmed diagnosis of Hodgkin’s lymphoma (53 people) or non-Hodgkin’s lymphoma (82 people) and the control group (135 people), apparently healthy individuals. Inclusion in groups occurred in parallel. All participants underwent a biochemical study of saliva to determine the content of amine nitrogen, the activity of enzymes (AlAT, AsAT, GGT, alkaline phosphatase). The patients of the main group were recruited on the basis of the Clinical Oncological Dispensary (Omsk, Russian Federation). Results . A significant increase in amino nitrogen (p < 0,0084), ALT (p < 0,0205), AST (p < 0,0047), GGT (p<0,0291) was found in the group of patients diagnosed with non-Hodgkin’s lymphoma, compared with subjects from the control group. Conclusion . A preliminary hypothesis was put forward that aminotransferases such as ALT, AST and GGT can be used not only as indicators of liver damage. A change in the activity of amine transferases with a simultaneous increase in the activity of amino nitrogen may reflect a pronounced redistribution of nitrogen in the body for the synthesis of new non-essential amino acids that are necessary for neoplastic cells as structural components for their growth and proliferation. This work also confirms that saliva is an informative diagnostic liquid that can be used in the routine practice of a clinician.","PeriodicalId":499018,"journal":{"name":"Russian Journal for Personalized Medicine","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136374896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：