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DYNAMICS OF REPRODUCTION OF GRAPE ROOTSTOCKS IN THE SYSTEM IN VITRO 葡萄砧木在离体系统中的繁殖动力学
Pub Date : 2024-01-25 DOI: 10.30679/2219-5335-2024-1-85-210-224
M.I. Grigorenko, I.A. Pavlova
In connection with the extension of areas under vineyards, it becomes necessary to use rootstocks, resistant to certain soil and climatic conditions (high content of active lime and salts in the soil, drought). Growth of the assortment of rootstocks available for propagation is one of the most important strategies of domestic nursery farming development, along with the production of improved planting material and better performance of its quality indicators. Today it is important to optimize clonal micro-propagation technology for promising grape rootstocks in order to increase net reproduction while maintaining the genetic identity of planting material. The purpose of the work was to study the dynamics of morphogenesis of grape rootstocks in the system in vitro to optimize the technology of clonal micro-propagation, taking into account the genetic specificity of particular genotypes. The study materials were the in vitro plants of grape rootstocks: Fercal clone 242, Ruggeri 140, and Gravesac (clones 11 and 12). The methods accepted in biotechnology and developed in the Breeding Department of the Institute Magarach were both used in the process of research. Cultivation was carried out on PG medium supplemented with NAA (α-naphthylacetic acid) 0.05 mg/l. The results showed that the dynamics of morphogenesis of rootstocks Ferсal clone 242, Gravesac clone 11 and Gravesac clone 12, analyzed on the basis of net propagation, did not differ significantly by varieties, with the exception of the second passage. The study of morphogenetic potential of Ruggeri 140 rootstock bud made it possible to reveal the dependence of net propagation on the line, it was especially noticeable in the first passage. Separate lines showed high net propagation in the second passage. The average indicator value for all lines was 2.59. In general for all rootstocks, similar results were obtained in successive passages, indicating a tendency for net propagation to be decreased. In order to increase the efficiency of technology, it is necessary to optimize the conditions of cultivation for industrial propagation, taking into account the genetic characteristics of every rootstock.
随着葡萄园面积的扩大,有必要使用对某些土壤和气候条件(土壤中活性石灰和盐分含量高、干旱)有抵抗力的砧木。增加可用于繁殖的砧木种类是国内苗圃农业发展的最重要战略之一,同时还能生产改良的种植材料,提高其质量指标。如今,对有前景的葡萄砧木进行克隆微繁殖技术优化,以增加净繁殖量,同时保持种植材料的遗传特性,具有重要意义。这项工作的目的是研究葡萄砧木在离体系统中的形态发生动态,以优化克隆微繁殖技术,同时考虑到特定基因型的遗传特异性。研究材料是葡萄砧木的离体植株:Fercal 克隆 242、Ruggeri 140 和 Gravesac(克隆 11 和 12)。研究过程中采用了生物技术中公认的方法和马格拉赫研究所育种部开发的方法。培养是在 PG 培养基上进行的,并添加了 0.05 毫克/升的 NAA(α-萘乙酸)。结果表明,根据净繁殖分析,砧木 Ferсal 克隆 242、Gravesac 克隆 11 和 Gravesac 克隆 12 的形态发生动态,除第二阶段外,各品种之间没有显著差异。对鲁格瑞 140 根茎芽的形态发生潜能进行的研究揭示了净繁殖对品系的依赖性,这种依赖性在第一阶段尤为明显。不同品系在第二阶段表现出较高的净繁殖能力。所有品系的平均指标值为 2.59。一般来说,所有砧木在连续种植过程中都获得了相似的结果,表明净繁殖率有下降的趋势。为了提高技术效率,有必要优化工业化繁殖的栽培条件,同时考虑到每种砧木的遗传特性。
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引用次数: 0
THE MAIN RESULTS OF SCIENTIFIC RESEARCH OF THE KRASNODAR RESEARCH INSTITUTE OF STORAGE AND PROCESSING OF AGRICULTURAL PRODUCTS – BRANCH OF THE FSBSI «NORTH CAUCASIAN FEDERAL SCIENTIFIC CENTER OF HORTICULTURE, VITICULTURE, WINEMAKING» IN 2023 2023 年克拉斯诺达尔农产品储藏和加工研究所--"北高加索联邦园艺、葡萄栽培和酿酒科学中心 "分部--科研的主要成果
Pub Date : 2024-01-25 DOI: 10.30679/2219-5335-2024-1-85-59-73
Anatolyevich Kupin Grigory, Viktorovna Yakovleva Tatiana, Pavlovna Viktorova Elena, Valerievna Lisovaya Ekaterina, Olegovich Semenikhin Semyon, Anatolyevna Shakhray Tatiana, Viktorovna Pershakova Tatiana
The main results of scientific research of the Krasnodar research Institute of storage and processing of agricultural products – branch of the FSBSI «North Caucasian Federal scientific center of horticulture, viticulture, winemaking», obtained in 2023 by scientists of the Institute in the implementation of the State order in 4 areas of scientific research, as well as data reflected the publication fecundity and patent activity. Research work was carried out in accordance with the PFSR in the Russian Federation for a long-term period (2021-2030) in the direction: 4.4.1 Storage and processing of agricultural products. Ensuring the priorities of the PFSR in the Russian Federation for the long term (2021-2030) provides for the need to conduct scientific research, the purpose of which is to develop algorithms for predicting the properties of biologically active substances based on molecular modeling methods, to identify patterns of controlled transformation of dietary fiber complexes, phospholipids and carotenoids using biotechnological and physico-chemical methods and, based on the identified patterns, to develop technologies for the production of beet pectin, modified phospholipids, lycopene and beta-carotene, as well as scientific research, aimed at identifying the mechanisms of microbiological, physiological, physico-chemical and biotechnological processes occurring in preparation for storage, storage and processing of vegetables (zucchini, eggplant, carrots and beets, tomatoes, leafy and cabbage vegetables), fruits (apples, grapes), for the development of resource-saving storage technologies based on the identified patterns. The results of scientific research have been published in 35 scientific articles, among them 11 articles in journals included in the core of the RSCI, in journals included in the list of the Higher Attestation Commission under the Ministry of Education and Science of the Russian Federation – 9 articles, in journals indexed in the international information and analytical citation systems Scopus – 3 articles, in materials and collections of scientific and practical conferences – 12 articles. 1 methodological recommendations have been published. Comprehensive score of publication performance: plan – 9.6, fact – 14.4. According to the results of the scientific research carried out, 6 patents of the Russian Federation for inventions were obtained and 2 certificates for computer programs, as well as 6 applications for alleged inventions were submitted to the FIIP.
克拉斯诺达尔农产品储藏与加工研究所--"北高加索联邦园艺、葡萄栽培和酿酒科学中心 "FSBSI 分支机构--的主要科研成果,是该研究所的科学家在 2023 年执行国家命令时在 4 个科研领域取得的成果,以及反映出版繁殖率和专利活动的数据。根据俄罗斯联邦长期(2021-2030 年)PFSR,开展了以下方向的研究工作:4.4.1 农产品的储存和加工。为确保俄罗斯联邦长期(2021-2030 年)PFSR 的优先事项,有必要开展科学研究,其目的 是根据分子建模方法开发预测生物活性物质特性的算法,利用生物技术和物理化学方法确定 膳食纤维复合物、磷脂和类胡萝卜素的受控转化模式,并根据确定的模式、开发甜菜果胶、改性磷脂、番茄红素和β-胡萝卜素的生产技术,以及旨在确定蔬菜(西葫芦、茄子、胡萝卜和甜菜、西红柿、叶菜类和卷心菜)、水果(苹果、葡萄)贮藏准备、贮藏和加工过程中发生的微生物、生理、物理化学和生物技术过程机制的科学研究,以便根据确定的模式开发节约资源的贮藏技术。科学研究成果已发表在 35 篇科学论文中,其中 11 篇发表在俄罗斯科学研究理事会核心期刊上, 9 篇发表在俄罗斯联邦教育和科学部下属高级鉴定委员会的期刊上,3 篇发表在国际信息和分析引文系统 Scopus 索引的期刊上,12 篇发表在科学和实践会议的资料和文集中。发表了 1 篇方法建议。出版业绩综合得分:计划 - 9.6,事实 - 14.4。根据所开展的科学研究结果,获得了 6 项俄罗斯联邦发明专利,2 项计算机程序证书,并向 FIIP 提交了 6 项所谓的发明申请。
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引用次数: 0
LOAD OF BUSHES OF SEEDLESS GRAPE VARIETIES BY SHOOTS AND BUNCHES: A REVIEW 无籽葡萄品种的枝串负载:综述
Pub Date : 2024-01-25 DOI: 10.30679/2219-5335-2024-1-85-239-252
A. Marmorshtein, V. Petrov, S. V. Mayborodin
The article considers the issue of loading bushes of seedless grape varieties with shoots and bunches in the world practice. The analysis is presented on the timing and length of pruning shoots, the load of bush by shoots, the load of bushes by bunches and methods for regulating the compactness of bunches of seedless grape varieties. The timing of pruning regulates the growth strength of shoots and the maturation time of early seedless grape varieties. Experiments on the timing of pruning often combined with subsequent treatment with growth regulators to increase yields. The length of the pruning of shoots affects the condition and death of the buds, the timing of the passage of phenological phases, productivity and quality of seedless varieties. Variety specificity and pruning dependence are noted: some varieties prefer short pruning (Perlett in India, White Seedless and Red Seedless in Iran), some – long (Beauty Seedless in India, Kishmish Batir in Uzbekistan, etc.). With an increase in the number of shoots per bush, yield capacity increase, however, the quality of grapes decreases, which also happens when the load of bunches increases. For the Jumba Seedless variety, despite the high performance with a low load of bushes with bunches, a higher one is recommended for an optimal ratio of productivity and quality. Thinning of bunches is also a common technique for regulating yield and quality, however, it is necessary to take into account the timing of thinning – too early removal of part of the inflorescences can lead to the formation of an excessively loose bunch, as in the BRS Vitoria variety in Brazil. Studies of the load of bushes of seedless grape varieties with shoots and bunches are relevant, especially in view of modern climate changes and increasing demand for high-quality products. Such studies are few in number in Russia, which requires closer study for the development of table seedless viticulture.
文章探讨了世界上无籽葡萄品种灌木丛的枝条和果穗负载问题。文章分析了修剪枝条的时间和长度、枝条对灌木丛的负载、灌木丛对葡萄串的负载以及调节无籽葡萄品种葡萄串紧密度的方法。修剪时间可调节枝条的生长强度和早熟无籽葡萄品种的成熟时间。有关修剪时间的试验通常与随后使用生长调节剂来提高产量相结合。枝条修剪的长短会影响芽的状态和死亡、物候期的通过时间、无籽品种的产量和质量。注意到品种的特殊性和修剪的依赖性:有些品种喜欢短修剪(印度的 Perlett、伊朗的白无籽和红无籽),有些则喜欢长修剪(印度的 Beauty Seedless、乌兹别克斯坦的 Kishmish Batir 等)。随着每丛枝条数量的增加,产量能力也会提高,但葡萄的质量会下降,当果穗的负载量增加时也会出现这种情况。对于 Jumba 无籽葡萄品种来说,尽管葡萄串数较少时产量较高,但为了达到最佳的产量和质量比,还是建议增加葡萄串数。疏剪果穗也是调节产量和质量的常用技术,但必须考虑到疏剪的时机--过早摘除部分花序会导致形成过于松散的果穗,巴西的 BRS Vitoria 品种就属于这种情况。对无籽葡萄品种枝条和果穗的负载量进行研究很有意义,尤其是考虑到现代气候变化和对高品质产品日益增长的需求。此类研究在俄罗斯为数不多,需要对其进行更深入的研究,以促进无籽葡萄栽培的发展。
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Fruit growing and viticulture of South Russia
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