Pub Date : 2024-06-01DOI: 10.29413/abs.2024-9.2.16
E. Taskina, A. A. Solovyova, V. A. Mudrov, S. V. Kharintseva
The prevalence rate of dry eye syndrome varies from 6.5 to 95 %. Diagnostic criteria are based on different methods and/or their combinations and are characterized by heterogeneity.The aim of the study. To identify the risk factors for the development of dry eye syndrome in order to create a technology for early diagnosis of the degree of the disease in young people without concomitant ocular and general somatic pathology.Materials and methods. Fifty patients aged 24 [22; 27] years were examined. We carried out an ophthalmological examination, including autorefractometry, visometry, biomicroscopy, the Norn test, a survey using the author’s questionnaire, and an assessment of the degree of dry eye syndrome using the Ocular Surface Disease Index (OSDI). Three study groups were formed: control group (OSDI = 0–13 points); group 1 – patients with OSDI = 14–22 points; group 2 – patients with OSDI > 22 points.Results. When examining presented independent variables, screen time had the highest normalized importance (100 %), followed by tear film breakup time (58.4 %), smoking (24.3 %), night shifts (22.5 %) and using soft contact lenses (11.1 %). The technology for early diagnosis of the degree of dry eye syndrome is implemented on the basis of a multilayer perceptron, the percentage of incorrect predictions during its training process was 8.0 %. The structure of the trained neural network included 8 input neurons (the value of screen time and tear film breakup time, the presence or absence of smoking, night shifts and/or the use of soft contact lenses), two hidden layers containing 3 and 2 units, respectively, and 3 output neurons.Conclusion. The proposed neural network has no difficulties in assessing the early diagnosis of the severity of dry eye syndrome and can be used in clinical practice.
{"title":"Possibilities of using neural network analysis in the diagnosis of dry eye syndrome","authors":"E. Taskina, A. A. Solovyova, V. A. Mudrov, S. V. Kharintseva","doi":"10.29413/abs.2024-9.2.16","DOIUrl":"https://doi.org/10.29413/abs.2024-9.2.16","url":null,"abstract":"The prevalence rate of dry eye syndrome varies from 6.5 to 95 %. Diagnostic criteria are based on different methods and/or their combinations and are characterized by heterogeneity.The aim of the study. To identify the risk factors for the development of dry eye syndrome in order to create a technology for early diagnosis of the degree of the disease in young people without concomitant ocular and general somatic pathology.Materials and methods. Fifty patients aged 24 [22; 27] years were examined. We carried out an ophthalmological examination, including autorefractometry, visometry, biomicroscopy, the Norn test, a survey using the author’s questionnaire, and an assessment of the degree of dry eye syndrome using the Ocular Surface Disease Index (OSDI). Three study groups were formed: control group (OSDI = 0–13 points); group 1 – patients with OSDI = 14–22 points; group 2 – patients with OSDI > 22 points.Results. When examining presented independent variables, screen time had the highest normalized importance (100 %), followed by tear film breakup time (58.4 %), smoking (24.3 %), night shifts (22.5 %) and using soft contact lenses (11.1 %). The technology for early diagnosis of the degree of dry eye syndrome is implemented on the basis of a multilayer perceptron, the percentage of incorrect predictions during its training process was 8.0 %. The structure of the trained neural network included 8 input neurons (the value of screen time and tear film breakup time, the presence or absence of smoking, night shifts and/or the use of soft contact lenses), two hidden layers containing 3 and 2 units, respectively, and 3 output neurons.Conclusion. The proposed neural network has no difficulties in assessing the early diagnosis of the severity of dry eye syndrome and can be used in clinical practice.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"135 47","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141281650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.29413/abs.2024-9.2.15
S. Radostev, L. A. Kolomiets, A. Shelekhov, R. A. Zubkov, D. Morikov, А. A. Medvednikov
Background. The high prevalence of advanced forms of cancer currently requires new treatment approaches. Only modern high-tech surgical procedures can provide means for improving the results of treatment.The aim. To study the results of treatment of patients with ovarian cancer with symptoms of peritoneal carcinomatosis using the HIPEC (Hyperthermic IntraPEritoneal Chemotherapy) method.Materials and methods. The study was conducted in Irkutsk Regional Cancer Center and included 88 patients. They were divided into 2 groups: group 1 – primary cytoreductive surgery (CRS) with the HIPEC method (41 patients); group 2 – primary cytoreductive surgery (47 patients). The most important criterion was the peritoneal cancer index (PCI). If the PCI was ≤ 14, we chose cytoreductive surgery with or without HIPEC.Results. PCI analysis showed differences in its values between the study groups (CRS – 10.17 ± 0.952; CRS + HIPEC – 12.93 ± 0.744; p = 0.002). There was an increase in length of stay in patients who underwent major surgeries in combination with HIPEC (21.8 ± 0.9 days versus 14.5 ± 0.6 days in the CRS group; p = 0.001). The CRS + HIPEC group had a longer duration of surgery (394.88 ± 19.935 min vs. 172.98 ± 11.514 min in the CRS group; p = 0.001). There was an increase in the overall percentage of postoperative complications in the CRS + HIPEC group – up to 26.8 % without statistically significant differences with the CRS group – 8.5 % (p = 0.082). The median time of relapse onset in the CRS + HIPEC group was 26 ± 4.3 months, while in the CRS group it was 18 ± 2.6 months.Conclusion. Using HIPEC method has proven its significance in increasing diseasefree survival.
{"title":"HIPEC application potential in the treatment of extensive carcinomatosis in ovarian cancer (retrospective study)","authors":"S. Radostev, L. A. Kolomiets, A. Shelekhov, R. A. Zubkov, D. Morikov, А. A. Medvednikov","doi":"10.29413/abs.2024-9.2.15","DOIUrl":"https://doi.org/10.29413/abs.2024-9.2.15","url":null,"abstract":"Background. The high prevalence of advanced forms of cancer currently requires new treatment approaches. Only modern high-tech surgical procedures can provide means for improving the results of treatment.The aim. To study the results of treatment of patients with ovarian cancer with symptoms of peritoneal carcinomatosis using the HIPEC (Hyperthermic IntraPEritoneal Chemotherapy) method.Materials and methods. The study was conducted in Irkutsk Regional Cancer Center and included 88 patients. They were divided into 2 groups: group 1 – primary cytoreductive surgery (CRS) with the HIPEC method (41 patients); group 2 – primary cytoreductive surgery (47 patients). The most important criterion was the peritoneal cancer index (PCI). If the PCI was ≤ 14, we chose cytoreductive surgery with or without HIPEC.Results. PCI analysis showed differences in its values between the study groups (CRS – 10.17 ± 0.952; CRS + HIPEC – 12.93 ± 0.744; p = 0.002). There was an increase in length of stay in patients who underwent major surgeries in combination with HIPEC (21.8 ± 0.9 days versus 14.5 ± 0.6 days in the CRS group; p = 0.001). The CRS + HIPEC group had a longer duration of surgery (394.88 ± 19.935 min vs. 172.98 ± 11.514 min in the CRS group; p = 0.001). There was an increase in the overall percentage of postoperative complications in the CRS + HIPEC group – up to 26.8 % without statistically significant differences with the CRS group – 8.5 % (p = 0.082). The median time of relapse onset in the CRS + HIPEC group was 26 ± 4.3 months, while in the CRS group it was 18 ± 2.6 months.Conclusion. Using HIPEC method has proven its significance in increasing diseasefree survival.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"13 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141279212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.29413/abs.2024-9.2.24
P. A. Markov, P. Eremin, I. Gilmutdinova, A. I. Greben, E. Kostromina, А. S. Sokolov, I. A. Artemyeva
It is well known that the acidity of the microenvironment has a significant impact on the functional activity of cells involved in tissue repair. To date, the effect of biomaterials containing components of the human dermis intercellular matrix on fibroblasts under acidosis has not been fully studied.The aim of the study. To evaluate the effect of a composite hydrogel containing dermal components on the functional activity of intact fibroblasts and fibroblasts with acid-induced dysfunction.Materials and methods. To simulate the physicochemical conditions of acute inflammation, human fibroblasts were incubated for an hour at 39 °C in a nutrient medium overacidified to pH = 6. The morphometric characteristics of human skin fibroblasts were assessed using light and fluorescence microscopy methods. The number of apoptotic cells and proliferation index were assessed using flow cytometry.Results. It has been established that exposure of fibroblasts to an acidic medium inhibits their adhesive properties and reduces the rate of cell growth. The introduction of hydrogel into a cell suspension with damaged cells restores adhesive properties and cell growth. In the fibroblast population, after acid exposure, the number of living cells decreases, and the number of apoptotic cells increases. Cultivation of damaged fibroblasts in the presence of a composite hydrogel increases the number of living cells in the population and reduces the number of apoptotic cells. Acid-induced fibroblast damage reduces the proliferative activity index. It was revealed that the introduction of hydrogel into the culture medium stimulates the proliferative activity of both intact and damaged fibroblasts. The results obtained indicate that a composite hydrogel consisting of extracellular matrix components is capable of restoring the functional activity of fibroblasts damaged as a result of exposure to an acidic medium. The results obtained can be used to create biomaterials that increase the efficiency of skin regeneration in cases of excessively intense acute inflammation.
{"title":"Effect of composite collagen containing hydrogel on the functional activity of fibroblasts after acid-induced in vitro injury","authors":"P. A. Markov, P. Eremin, I. Gilmutdinova, A. I. Greben, E. Kostromina, А. S. Sokolov, I. A. Artemyeva","doi":"10.29413/abs.2024-9.2.24","DOIUrl":"https://doi.org/10.29413/abs.2024-9.2.24","url":null,"abstract":"It is well known that the acidity of the microenvironment has a significant impact on the functional activity of cells involved in tissue repair. To date, the effect of biomaterials containing components of the human dermis intercellular matrix on fibroblasts under acidosis has not been fully studied.The aim of the study. To evaluate the effect of a composite hydrogel containing dermal components on the functional activity of intact fibroblasts and fibroblasts with acid-induced dysfunction.Materials and methods. To simulate the physicochemical conditions of acute inflammation, human fibroblasts were incubated for an hour at 39 °C in a nutrient medium overacidified to pH = 6. The morphometric characteristics of human skin fibroblasts were assessed using light and fluorescence microscopy methods. The number of apoptotic cells and proliferation index were assessed using flow cytometry.Results. It has been established that exposure of fibroblasts to an acidic medium inhibits their adhesive properties and reduces the rate of cell growth. The introduction of hydrogel into a cell suspension with damaged cells restores adhesive properties and cell growth. In the fibroblast population, after acid exposure, the number of living cells decreases, and the number of apoptotic cells increases. Cultivation of damaged fibroblasts in the presence of a composite hydrogel increases the number of living cells in the population and reduces the number of apoptotic cells. Acid-induced fibroblast damage reduces the proliferative activity index. It was revealed that the introduction of hydrogel into the culture medium stimulates the proliferative activity of both intact and damaged fibroblasts. The results obtained indicate that a composite hydrogel consisting of extracellular matrix components is capable of restoring the functional activity of fibroblasts damaged as a result of exposure to an acidic medium. The results obtained can be used to create biomaterials that increase the efficiency of skin regeneration in cases of excessively intense acute inflammation.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"7 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141274622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.29413/abs.2024-9.1.15
D. A. Popova, Y. V. Kursakova
Demodicosis occupies a leading position among all dermatoses. According to ICD-10, it is not distinguished as a separate disease; it belongs to the class of parasitic diseases. The relevance of studying the problem of ophthalmodemodecosis is caused by its high prevalence and contagiousness, chronic course, an open issue of the role of the Demodex mite in the occurrence of inflammatory eye diseases, as well as the lack of effective methods for treating and preventing this pathology. In addition, Demodex causes discomfort and contributes to the occurrence of cosmetic defects, which in turn worsens the patient’s quality of life.The aim. To present a review of the literature data and our own results of laboratory diagnostics of ophthalmodemodecosis.Materials and methods. The article presents clinical cases of demodectic eyelid lesions with different disease outcomes in case of similar treatment. Laboratory diagnostics included drawing up an acarogram. Epilated eyelashes were used as a material for detecting mites on eyelids. Counting of individuals was carried out using light microscopy; all forms of mite development were taken into account. The work presents the statistics on the frequency of examination of patients with suspected demodicosis at different times of the year, confirming the seasonality of this disease. The literature review included data on the history of studying the Demodex mite, existing hypotheses and theories about its pathogenesis, as well as the information on domestic and foreign methods of treating ophthalmodecosis, including modern hardware techniques.Results. Demodex mites play a significant role in the development of blepharitis and blepharoconjunctivitis. It is important to consider that demodicosis can occur against the background of inflammatory eye diseases of another etiology. Therapy for ophthalmodemodecosis currently remains complex, lengthy and ineffective. When assessing the results of an acarogram, any detected stages of a mite are clinically significant, and there isn’t a direct relationship between the number of detected mites and the severity of clinical manifestations in all cases.
{"title":"Some aspects of laboratory diagnostics of ophthalmodemodecosis","authors":"D. A. Popova, Y. V. Kursakova","doi":"10.29413/abs.2024-9.1.15","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.15","url":null,"abstract":"Demodicosis occupies a leading position among all dermatoses. According to ICD-10, it is not distinguished as a separate disease; it belongs to the class of parasitic diseases. The relevance of studying the problem of ophthalmodemodecosis is caused by its high prevalence and contagiousness, chronic course, an open issue of the role of the Demodex mite in the occurrence of inflammatory eye diseases, as well as the lack of effective methods for treating and preventing this pathology. In addition, Demodex causes discomfort and contributes to the occurrence of cosmetic defects, which in turn worsens the patient’s quality of life.The aim. To present a review of the literature data and our own results of laboratory diagnostics of ophthalmodemodecosis.Materials and methods. The article presents clinical cases of demodectic eyelid lesions with different disease outcomes in case of similar treatment. Laboratory diagnostics included drawing up an acarogram. Epilated eyelashes were used as a material for detecting mites on eyelids. Counting of individuals was carried out using light microscopy; all forms of mite development were taken into account. The work presents the statistics on the frequency of examination of patients with suspected demodicosis at different times of the year, confirming the seasonality of this disease. The literature review included data on the history of studying the Demodex mite, existing hypotheses and theories about its pathogenesis, as well as the information on domestic and foreign methods of treating ophthalmodecosis, including modern hardware techniques.Results. Demodex mites play a significant role in the development of blepharitis and blepharoconjunctivitis. It is important to consider that demodicosis can occur against the background of inflammatory eye diseases of another etiology. Therapy for ophthalmodemodecosis currently remains complex, lengthy and ineffective. When assessing the results of an acarogram, any detected stages of a mite are clinically significant, and there isn’t a direct relationship between the number of detected mites and the severity of clinical manifestations in all cases.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"14 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140375620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.29413/abs.2024-9.1.14
E. V. Arkhipov, V. A. Zaika, S. V. Kuzmin, D. Samsonov, M. D. Nefedieva, K. R. Zhuravskaya
The aim. To present a clinical case of surgical treatment of parasitic invasion of the vitreous body caused by dirofilaria.Material and methods. One patient with intraocular dirofilariasis got operated underwent surgical treatment including vitrectomy, phacoemulsification with implantation of an intraocular lens. At the stage of vitrectomy, a whole helminth was removed from the vitreal cavity using collet tweezers for subsequent typing. The uncorrected visual acuity of the right eye at the time of treatment was 0.4, of the left eye – 0.45.Results. The postoperative period had no signs of an active inflammatory reaction. 4 months after surgical treatment, at a follow-up visit, visual acuity of the left eye reached 1.0. According to the parasitological study, a female Dirofilaria repens was identified. In the postoperative period, the areas of pronounced chorioretinal atrophy in the peripheral parts of the retina were visualized, which may be a consequence of mechanical contact of the parasite or the toxic effects of its metabolic products.Conclusion. This clinical example demonstrates the possibility of infection with the ocular form of dirofilariasis in a region that is atypical for the presence of this helminth. Despite the positive outcome of the disease, in the presented patient, the long-term presence of the parasite in the vitreal cavity led to the formation of chorioretinal atrophy in the peripheral retina, which confirms the need for timely diagnosis and surgical treatment.
{"title":"Intraocular dirofilariasis: Surgical approaches, features of the clinical course (clinical case)","authors":"E. V. Arkhipov, V. A. Zaika, S. V. Kuzmin, D. Samsonov, M. D. Nefedieva, K. R. Zhuravskaya","doi":"10.29413/abs.2024-9.1.14","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.14","url":null,"abstract":"The aim. To present a clinical case of surgical treatment of parasitic invasion of the vitreous body caused by dirofilaria.Material and methods. One patient with intraocular dirofilariasis got operated underwent surgical treatment including vitrectomy, phacoemulsification with implantation of an intraocular lens. At the stage of vitrectomy, a whole helminth was removed from the vitreal cavity using collet tweezers for subsequent typing. The uncorrected visual acuity of the right eye at the time of treatment was 0.4, of the left eye – 0.45.Results. The postoperative period had no signs of an active inflammatory reaction. 4 months after surgical treatment, at a follow-up visit, visual acuity of the left eye reached 1.0. According to the parasitological study, a female Dirofilaria repens was identified. In the postoperative period, the areas of pronounced chorioretinal atrophy in the peripheral parts of the retina were visualized, which may be a consequence of mechanical contact of the parasite or the toxic effects of its metabolic products.Conclusion. This clinical example demonstrates the possibility of infection with the ocular form of dirofilariasis in a region that is atypical for the presence of this helminth. Despite the positive outcome of the disease, in the presented patient, the long-term presence of the parasite in the vitreal cavity led to the formation of chorioretinal atrophy in the peripheral retina, which confirms the need for timely diagnosis and surgical treatment.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"29 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140373901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.29413/abs.2024-9.1.21
T. Kazakova, O. Marshinskaia, S. Notova
Background. The presence of increased manganese concentrations in soils and water intakes areas for drinking and household purposes in a number of the Russian Federation subjects indicates the chronic influence of this metal on all segments of the population. This situation is aggravated by violation of the optimal nutrition principles, which leads to changes in the organism absorption of micronutrients. In this regard, the study of the elemental status of an organism against the background of manganese exposure is of particular importance.The aim. To study the effects of subchronic exposure to manganese on the mineral status of Wistar rats.Materials and methods. To conduct the study, 20 mature rats were selected, from which two groups were formed – control (n = 10) and experimental (n = 10). Animals in the control group received a general diet, animals in the experimental group received a diet with additional administration of manganese sulfate at a dose of 1433 mg/kg for 28 days. At the end of the preparatory period, blood and brain samples were taken to determine the content of chemical elements using inductively coupled plasma mass spectrometry and of metal-ligand manganese forms using high-performance liquid chromatography combined with inductively coupled plasma mass spectrometry.Results. It has been established that subchronic oral exposure to manganese leads to an increase in the content of this microelement in the blood serum and to a decrease in the levels of calcium, potassium, magnesium, iron and copper. In the cerebral cortex, the level of manganese, lead, mercury and strontium increases against the background of a decrease in iron and iodine levels. An increase in the gross content of manganese in blood serum leads to an overload of the main high-molecular carriers and initiates the formation of low-molecular forms of manganese.Conclusion. Subchronic oral exposure to manganese leads to the accumulation of this microelement in the body of animals and to the development of an imbalance of a number of macro- and microelements.
{"title":"Effect of subchronic exposure to manganese on mineral metabolism in Wistar rats","authors":"T. Kazakova, O. Marshinskaia, S. Notova","doi":"10.29413/abs.2024-9.1.21","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.21","url":null,"abstract":"Background. The presence of increased manganese concentrations in soils and water intakes areas for drinking and household purposes in a number of the Russian Federation subjects indicates the chronic influence of this metal on all segments of the population. This situation is aggravated by violation of the optimal nutrition principles, which leads to changes in the organism absorption of micronutrients. In this regard, the study of the elemental status of an organism against the background of manganese exposure is of particular importance.The aim. To study the effects of subchronic exposure to manganese on the mineral status of Wistar rats.Materials and methods. To conduct the study, 20 mature rats were selected, from which two groups were formed – control (n = 10) and experimental (n = 10). Animals in the control group received a general diet, animals in the experimental group received a diet with additional administration of manganese sulfate at a dose of 1433 mg/kg for 28 days. At the end of the preparatory period, blood and brain samples were taken to determine the content of chemical elements using inductively coupled plasma mass spectrometry and of metal-ligand manganese forms using high-performance liquid chromatography combined with inductively coupled plasma mass spectrometry.Results. It has been established that subchronic oral exposure to manganese leads to an increase in the content of this microelement in the blood serum and to a decrease in the levels of calcium, potassium, magnesium, iron and copper. In the cerebral cortex, the level of manganese, lead, mercury and strontium increases against the background of a decrease in iron and iodine levels. An increase in the gross content of manganese in blood serum leads to an overload of the main high-molecular carriers and initiates the formation of low-molecular forms of manganese.Conclusion. Subchronic oral exposure to manganese leads to the accumulation of this microelement in the body of animals and to the development of an imbalance of a number of macro- and microelements.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"23 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140374764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.29413/abs.2024-9.1.17
I. Tikhonova, N. S. Shipova, Yu. V. Misiyuk
Studying stress factors of childbirth intentions is an important parameter in population development policy making. There is no data on the comparison of the relative importance of different types of determinants and stress inducing factors of parenting in the scientific literature.The aim. To study the factors determining the ideas about the stressfulness of the parental role among modern youth.Methods. The sample consisted of 364 students with no parenting experience. Three groups were identified, differing in their orientation towards having children: a group with a focus on single-child parenting (257 people); group with childfree focus (32 people); group with a focus on multi-child parenting (75 people). The following methods were used: socio-demographic questionnaire; “Parental Stress Scale” (J.O. Berry, W. Jones, 1995; adapted by Yu.V. Misiyuk, I.V. Tikhonova, 2022); “Intensive Parenting Attitudes Questionnaire (M. Liss, H.H. Schiffrin, V.H. Mackintosh, H. Miles-McLean, M.J. Erchull, 2013; adapted by Yu.V. Misiyuk, 2022); express version of the “World Assumptions Scale” (R. Yanoff-Bulman; modified by M.A. Padun, A.V. Kotelnikova; author’s semi-structured interview.Results. We analyzed the ideas about the stress that is associated with fulfilling the parental role. Parameters that potentially act as the factors determining the stressfulness of parenthood are considered. The specificity of ideas on parental stress and its determinants is analyzed in accordance with the dominant orientation towards childbearing.Conclusion. Ideas about the stressfulness of the parental role are determined by objective (age, status of personal relationships, education) and subjective (assessment of family relationship, attitudes towards the need to stimulate the child’s development, essentialism and satisfaction from raising children) factors, but subjective ones have the greatest weight. The specificity of the determination of ideas about parental stress depending on the orientation towards childbearing has been revealed.
{"title":"Ideas about the stressfulness of the parental role in potential parents: objective and subjective determinants","authors":"I. Tikhonova, N. S. Shipova, Yu. V. Misiyuk","doi":"10.29413/abs.2024-9.1.17","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.17","url":null,"abstract":"Studying stress factors of childbirth intentions is an important parameter in population development policy making. There is no data on the comparison of the relative importance of different types of determinants and stress inducing factors of parenting in the scientific literature.The aim. To study the factors determining the ideas about the stressfulness of the parental role among modern youth.Methods. The sample consisted of 364 students with no parenting experience. Three groups were identified, differing in their orientation towards having children: a group with a focus on single-child parenting (257 people); group with childfree focus (32 people); group with a focus on multi-child parenting (75 people). The following methods were used: socio-demographic questionnaire; “Parental Stress Scale” (J.O. Berry, W. Jones, 1995; adapted by Yu.V. Misiyuk, I.V. Tikhonova, 2022); “Intensive Parenting Attitudes Questionnaire (M. Liss, H.H. Schiffrin, V.H. Mackintosh, H. Miles-McLean, M.J. Erchull, 2013; adapted by Yu.V. Misiyuk, 2022); express version of the “World Assumptions Scale” (R. Yanoff-Bulman; modified by M.A. Padun, A.V. Kotelnikova; author’s semi-structured interview.Results. We analyzed the ideas about the stress that is associated with fulfilling the parental role. Parameters that potentially act as the factors determining the stressfulness of parenthood are considered. The specificity of ideas on parental stress and its determinants is analyzed in accordance with the dominant orientation towards childbearing.Conclusion. Ideas about the stressfulness of the parental role are determined by objective (age, status of personal relationships, education) and subjective (assessment of family relationship, attitudes towards the need to stimulate the child’s development, essentialism and satisfaction from raising children) factors, but subjective ones have the greatest weight. The specificity of the determination of ideas about parental stress depending on the orientation towards childbearing has been revealed.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"6 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140374896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.29413/abs.2024-9.1.23
Z.V. Dzampaeva, F. Datieva, E. Takoeva, M. Nartikoeva
The aim of the study. To assess the possibility of correction and prevention of behavioral disorders in rats with metabolic syndrome using a complex phytoadaptogen (CPA).Material and methods. The experiment was carried out on 30 male Wistar rats randomized into 3 groups: group 1 – control; group 2 – metabolic syndrome (MS); group 3 – treatment of metabolic syndrome using CPA. In groups 2 and 3, animals were on a high-carbohydrate and high-fat diet for 16 weeks. Group 3 received CPA for 14 days in drinking water after 16 weeks of a diet. CPA consists of official tinctures of Glycyrrhiza glabra, Rhodiola rosea, Acantopanax senticosus at a ratio of 1:2:1. Behavior was analyzed through the “open field” test using Realtimer software (Open Science, Russia). Data were analyzed using GraphPad Prism 8.03 software (GraphPad, USA).Results. The experiment proved that metabolic syndrome is accompanied by increased anxiety (decreased horizontal (p = 0.017) and vertical (p = 0.017) motor activity) and fear (increased periods of immobility (p = 0.011)) in the open field. When corrected with a complex phytoadaptogen, the time spent in the open and closed arms of the maze did not differ statistically significantly from the values of similar control indicators.Conclusion. Based on the data obtained in the group 3 (no statistically significant differences with control) – decreased manifestations of fear and anxiety (increased orientation and research activity) – we can talk about the effectiveness of complex phytoadaptogens as an anxiolytic. The mechanisms underlying this result remain to be explored, emphasizing the role of the autonomic nervous system, leptin and ghrelin in behavior and the influence of complex phytoadaptogens on them.
{"title":"Prevention and correction of behavioral disorders in rats with metabolic syndrome using a complex phytoadaptogen","authors":"Z.V. Dzampaeva, F. Datieva, E. Takoeva, M. Nartikoeva","doi":"10.29413/abs.2024-9.1.23","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.23","url":null,"abstract":"The aim of the study. To assess the possibility of correction and prevention of behavioral disorders in rats with metabolic syndrome using a complex phytoadaptogen (CPA).Material and methods. The experiment was carried out on 30 male Wistar rats randomized into 3 groups: group 1 – control; group 2 – metabolic syndrome (MS); group 3 – treatment of metabolic syndrome using CPA. In groups 2 and 3, animals were on a high-carbohydrate and high-fat diet for 16 weeks. Group 3 received CPA for 14 days in drinking water after 16 weeks of a diet. CPA consists of official tinctures of Glycyrrhiza glabra, Rhodiola rosea, Acantopanax senticosus at a ratio of 1:2:1. Behavior was analyzed through the “open field” test using Realtimer software (Open Science, Russia). Data were analyzed using GraphPad Prism 8.03 software (GraphPad, USA).Results. The experiment proved that metabolic syndrome is accompanied by increased anxiety (decreased horizontal (p = 0.017) and vertical (p = 0.017) motor activity) and fear (increased periods of immobility (p = 0.011)) in the open field. When corrected with a complex phytoadaptogen, the time spent in the open and closed arms of the maze did not differ statistically significantly from the values of similar control indicators.Conclusion. Based on the data obtained in the group 3 (no statistically significant differences with control) – decreased manifestations of fear and anxiety (increased orientation and research activity) – we can talk about the effectiveness of complex phytoadaptogens as an anxiolytic. The mechanisms underlying this result remain to be explored, emphasizing the role of the autonomic nervous system, leptin and ghrelin in behavior and the influence of complex phytoadaptogens on them.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"16 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140374350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.29413/abs.2024-9.1.20
S. Michurina, S. I. Kolesnikov, I. Ishchenko, S. Arkhipov
Background. There is growing interest in determining the role of melatonin in the regulation of proliferation and apoptosis of ovarian cells at various diseases and destabilizing influences. It is believed that the choice between the implementation of a cell death or survival program determines the ratio of anti-apoptotic and pro-apoptotic proteins.The aim. To identify the effect ofmelatonin onthe expression ofanti-apoptotic Bcl-2 and pro-apoptotic Bad and the Bcl-2/Bad ratio in the ovarian luteocytes of Wistar rats in the acute (day 3) and recovery (days 7 and 14) periods after a single exposure to experimental hyperthermia.Methods. Warming up took no more than 17 minutes. Melatonin was injected subcutaneously (0.1 mg in 0.2 ml of physiological solution) for 3 days after experimental hyperthermia. Comparison groups included rats with physiological solution injection (control) and animals after experimental hyperthermia + physiological solution injection. The Bad and Bcl-2 expression was determined immunohistochemically on days 3, 7 and 14 after experimental hyperthermia + physiological solution or melatonin injection.Results. On the day 3 after experimental hyperthermia, the effect of the hormone was not detected. A week after experimental hyperthermia + melatonin injection, the Bad expression area decreased more significantly than in rats after experimental hyperthermia + physiological solution injection, which led to an increase in Bcl-2/ Bad ratio. This indicated an increase in anti-apoptotic protection, blocking the development of the internal apoptosis pathway at this time. 2 weeks after experimental hyperthermia + physiological solution injection, the Bcl-2 area decreased more significantly than the Bad area. As a result, the Bcl-2/Bad ratio decreased almost 2-fold compared to the control group. This indicated the activation of the “mitochondrial branch” of luteocyte apoptosis. 2 weeks after experimental hyperthermia + melatonin injection, the Bad and Bcl-2 areas decreased synchronously, which restored Bcl-2/ Bad to control values.Conclusion. The melatonin injection after experimental hyperthermia shifts the ratio of Bcl-2/Bad expression areas towards an increase in anti-apoptotic Bcl2 already a week after the recovery period and promotes earlier normalization of Bcl-2/Bad to physiological levels (as early as 2 weeks after experimental hyperthermia + melatonin injection).
{"title":"The effect of melatonin on the Bcl-2 and Bad proteins expression in ovarian corpus luteum cells after exposure to experimental hyperthermia","authors":"S. Michurina, S. I. Kolesnikov, I. Ishchenko, S. Arkhipov","doi":"10.29413/abs.2024-9.1.20","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.20","url":null,"abstract":"Background. There is growing interest in determining the role of melatonin in the regulation of proliferation and apoptosis of ovarian cells at various diseases and destabilizing influences. It is believed that the choice between the implementation of a cell death or survival program determines the ratio of anti-apoptotic and pro-apoptotic proteins.The aim. To identify the effect ofmelatonin onthe expression ofanti-apoptotic Bcl-2 and pro-apoptotic Bad and the Bcl-2/Bad ratio in the ovarian luteocytes of Wistar rats in the acute (day 3) and recovery (days 7 and 14) periods after a single exposure to experimental hyperthermia.Methods. Warming up took no more than 17 minutes. Melatonin was injected subcutaneously (0.1 mg in 0.2 ml of physiological solution) for 3 days after experimental hyperthermia. Comparison groups included rats with physiological solution injection (control) and animals after experimental hyperthermia + physiological solution injection. The Bad and Bcl-2 expression was determined immunohistochemically on days 3, 7 and 14 after experimental hyperthermia + physiological solution or melatonin injection.Results. On the day 3 after experimental hyperthermia, the effect of the hormone was not detected. A week after experimental hyperthermia + melatonin injection, the Bad expression area decreased more significantly than in rats after experimental hyperthermia + physiological solution injection, which led to an increase in Bcl-2/ Bad ratio. This indicated an increase in anti-apoptotic protection, blocking the development of the internal apoptosis pathway at this time. 2 weeks after experimental hyperthermia + physiological solution injection, the Bcl-2 area decreased more significantly than the Bad area. As a result, the Bcl-2/Bad ratio decreased almost 2-fold compared to the control group. This indicated the activation of the “mitochondrial branch” of luteocyte apoptosis. 2 weeks after experimental hyperthermia + melatonin injection, the Bad and Bcl-2 areas decreased synchronously, which restored Bcl-2/ Bad to control values.Conclusion. The melatonin injection after experimental hyperthermia shifts the ratio of Bcl-2/Bad expression areas towards an increase in anti-apoptotic Bcl2 already a week after the recovery period and promotes earlier normalization of Bcl-2/Bad to physiological levels (as early as 2 weeks after experimental hyperthermia + melatonin injection).","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"58 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140376342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.29413/abs.2024-9.1.19
T. V. Siraeva, O. G. Komissarova, V. V. Romanov
Background. Despite the visible successes of domestic phthisiology at the present time, the problem of medical rehabilitation of patients with tuberculosis remains relevant. In recent years, approaches to medical rehabilitation of patients with various diseases have changed significantly, which entail the need to consider a complex of rehabilitation measures for patients with tuberculosis from the perspective of the Procedures for organizing medical rehabilitation in adults and children and their integration into phthisiatric practice.The aim. To study the organizational aspects of medical rehabilitation of patients with respiratory tuberculosis in the world and the Russian Federation.Methods. We carried out an analysis of domestic and foreign literature, regulatory documents on the organization of rehabilitation for tuberculosis patients for 2018–2023 in electronic databases PubMed/Medline, Google Scholar using terms “tuberculosis, pulmonary/rehabilitation” in English and Russian languages.The results show a growing amount of factual information demonstrating the positive effect of pulmonary rehabilitation in patients with respiratory diseases, including tuberculosis. The analysis revealed defects in the organization of the medical rehabilitation system in the structure of medical care for tuberculosis patients in the Russian Federation. This concerns problems of routing, phasing, organizational models, human and material resources, standardization of the main components of the rehabilitation process, the significance and effectiveness of certain rehabilitation measures, which leads to low availability of rehabilitation care for tuberculosis patients. Deficiencies in the regulatory framework prevent the integration of medical rehabilitation into the practice of TB services.Conclusion. Modern issues of organizing rehabilitation care for patients with tuberculosis require further study and improvement. The development of a system of medical rehabilitation of patients with tuberculosis helps to increase the effectiveness of treatment, to reduce the number of complications, disability, mortality due to tuberculosis, and to increase the duration and quality of life of patients.
{"title":"Organizational aspects of medical rehabilitation of patients with respiratory tuberculosis","authors":"T. V. Siraeva, O. G. Komissarova, V. V. Romanov","doi":"10.29413/abs.2024-9.1.19","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.19","url":null,"abstract":"Background. Despite the visible successes of domestic phthisiology at the present time, the problem of medical rehabilitation of patients with tuberculosis remains relevant. In recent years, approaches to medical rehabilitation of patients with various diseases have changed significantly, which entail the need to consider a complex of rehabilitation measures for patients with tuberculosis from the perspective of the Procedures for organizing medical rehabilitation in adults and children and their integration into phthisiatric practice.The aim. To study the organizational aspects of medical rehabilitation of patients with respiratory tuberculosis in the world and the Russian Federation.Methods. We carried out an analysis of domestic and foreign literature, regulatory documents on the organization of rehabilitation for tuberculosis patients for 2018–2023 in electronic databases PubMed/Medline, Google Scholar using terms “tuberculosis, pulmonary/rehabilitation” in English and Russian languages.The results show a growing amount of factual information demonstrating the positive effect of pulmonary rehabilitation in patients with respiratory diseases, including tuberculosis. The analysis revealed defects in the organization of the medical rehabilitation system in the structure of medical care for tuberculosis patients in the Russian Federation. This concerns problems of routing, phasing, organizational models, human and material resources, standardization of the main components of the rehabilitation process, the significance and effectiveness of certain rehabilitation measures, which leads to low availability of rehabilitation care for tuberculosis patients. Deficiencies in the regulatory framework prevent the integration of medical rehabilitation into the practice of TB services.Conclusion. Modern issues of organizing rehabilitation care for patients with tuberculosis require further study and improvement. The development of a system of medical rehabilitation of patients with tuberculosis helps to increase the effectiveness of treatment, to reduce the number of complications, disability, mortality due to tuberculosis, and to increase the duration and quality of life of patients.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"100 47","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140377607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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