Background: Communities in the Danau Tundai Area, Palangka Raya City, Central Kalimantan Province, Indonesia still use peat water for their daily water needs. The use of peat water is one of the things that need to be considered because disease transmission can occur through water with poor quality and will cause health problems in the Danau Tundai community.Objective: This study aimed to determine the quality of peat water in the Danau Tundai Area and to identify the association between the quality of peat water and public health problems in the Danau Tundai AreaMethods: Water samples taken were analyzed physically, chemically, and biologically by government water quality regulations. The primary data source in this study was a questionnaire to obtain data on peat water use and health problems among the people of Danau TundaiResults: Results indicated that peat water quality of the Danau Tundai Area didn’t meet the Ministry of Health standard for drinking water because the physical appearance was brown, tasted, and smells. The chemical analyses also showed that the levels of KMnO4 were 257.5 mg/L, Fe 0.66 mg/L, pH 4.02, Dissolved Oxygen (DO) 5.9 mg/L, Biological Oxygen Demand (BOD) 18.6 mg/L, and Chemical Oxygen Demand (COD) 156.9 mg/L. Those levels were higher than the cut-off points set by Ministry of Health. The public health problems that mostly experienced was itching (75.3%), stomachache (66.7%), and yellow teeth (61.3%). Simple linear regression showed a positive significant association between quality of peat water (based on individual perception) and public health problems in the Danau Tundai area (p = 0.000).Conclusion: Peat water quality of the Danau Tundai Area didn’t meet the Ministry of Health standard for drinking water, and the quality of peat water had a significant association with health problems Danau Tundai communities.
{"title":"Quality of the peat water and its association with public health problems in the community of the Danau Tundai area","authors":"Nawan Nawan, Hopelyanza Priskila, Herlina Eka Shinta, Septi Handayani, Ravenalla Abdurahman","doi":"10.20885/jkki.vol14.iss2.art7","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art7","url":null,"abstract":"Background: Communities in the Danau Tundai Area, Palangka Raya City, Central Kalimantan Province, Indonesia still use peat water for their daily water needs. The use of peat water is one of the things that need to be considered because disease transmission can occur through water with poor quality and will cause health problems in the Danau Tundai community.Objective: This study aimed to determine the quality of peat water in the Danau Tundai Area and to identify the association between the quality of peat water and public health problems in the Danau Tundai AreaMethods: Water samples taken were analyzed physically, chemically, and biologically by government water quality regulations. The primary data source in this study was a questionnaire to obtain data on peat water use and health problems among the people of Danau TundaiResults: Results indicated that peat water quality of the Danau Tundai Area didn’t meet the Ministry of Health standard for drinking water because the physical appearance was brown, tasted, and smells. The chemical analyses also showed that the levels of KMnO4 were 257.5 mg/L, Fe 0.66 mg/L, pH 4.02, Dissolved Oxygen (DO) 5.9 mg/L, Biological Oxygen Demand (BOD) 18.6 mg/L, and Chemical Oxygen Demand (COD) 156.9 mg/L. Those levels were higher than the cut-off points set by Ministry of Health. The public health problems that mostly experienced was itching (75.3%), stomachache (66.7%), and yellow teeth (61.3%). Simple linear regression showed a positive significant association between quality of peat water (based on individual perception) and public health problems in the Danau Tundai area (p = 0.000).Conclusion: Peat water quality of the Danau Tundai Area didn’t meet the Ministry of Health standard for drinking water, and the quality of peat water had a significant association with health problems Danau Tundai communities.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.20885/jkki.vol14.iss2.art14
Al Hafiz, Wahyu Julianda
Maxillofacial trauma is a common clinical situation in emergency rooms. Lesions involving the maxillofacial complex pose a significant public health challenge, not only due to their costs but also because of the potential functional and aesthetic issues that patients may experience. While some fractures are more suitable for delayed treatment, others necessitate immediate attention, requiring intervention within 24 hours of the trauma occurrence. Different types of reduction and fixation exist depending on the patient’s age, location, type of fracture, and the surgeon’s preferences. We reported a case of a 20-year-old female patient diagnosed with Le Fort I bilateral fracture and closed nasal fracture followed by saddle nose. The patient was managed with open reduction internal fixation and closed reduction nasal bone. The saddle nose in the patient was managed four months later by augmentation rhinoplasty with rib cartilage and pearl fat graft. Proper management of Le Fort I fracture and nasal fracture accompanied with saddle nose by open reduction internal fixation and augmentation rhinoplasty with rib cartilage and pearl fat graft can restore occlusion and improve symmetrical, proportional facial aesthetics, which is the indicators of a successful holistic maxillofacial trauma management.
颌面部创伤是急诊室常见的临床症状。涉及颌面部复合体的病变对公共卫生构成了重大挑战，这不仅是因为其费用高昂，还因为患者可能会出现功能和美观方面的问题。有些骨折更适合延迟治疗，而有些骨折则需要立即治疗，需要在创伤发生后 24 小时内进行干预。根据患者的年龄、骨折部位、骨折类型和外科医生的偏好，有不同的复位和固定方式。我们报告了一例 20 岁女性患者的病例，她被诊断为 Le Fort I 双侧骨折和鼻骨闭合性骨折，随后出现鞍鼻。患者接受了开放复位内固定术和鼻骨闭合复位术。四个月后，该患者通过肋软骨和珍珠脂肪移植隆鼻术治疗了鞍鼻。通过开放复位内固定术和肋软骨及珍珠脂肪移植隆鼻术，妥善处理 Le Fort I 型骨折和鼻骨骨折伴鞍鼻，可以恢复咬合，改善对称、比例协调的面部美观，这是颌面创伤整体治疗成功的标志。
{"title":"Le Fort I and nasal fracture, primary reduction, and secondary revision saddle nose: A case report","authors":"Al Hafiz, Wahyu Julianda","doi":"10.20885/jkki.vol14.iss2.art14","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art14","url":null,"abstract":"Maxillofacial trauma is a common clinical situation in emergency rooms. Lesions involving the maxillofacial complex pose a significant public health challenge, not only due to their costs but also because of the potential functional and aesthetic issues that patients may experience. While some fractures are more suitable for delayed treatment, others necessitate immediate attention, requiring intervention within 24 hours of the trauma occurrence. Different types of reduction and fixation exist depending on the patient’s age, location, type of fracture, and the surgeon’s preferences. We reported a case of a 20-year-old female patient diagnosed with Le Fort I bilateral fracture and closed nasal fracture followed by saddle nose. The patient was managed with open reduction internal fixation and closed reduction nasal bone. The saddle nose in the patient was managed four months later by augmentation rhinoplasty with rib cartilage and pearl fat graft. Proper management of Le Fort I fracture and nasal fracture accompanied with saddle nose by open reduction internal fixation and augmentation rhinoplasty with rib cartilage and pearl fat graft can restore occlusion and improve symmetrical, proportional facial aesthetics, which is the indicators of a successful holistic maxillofacial trauma management.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cutaneous tuberculosis is a skin disorder caused by Mycobacterium tuberculosis, commonly classified according to its spreading route into endogenous, exogenous, and hematogenous, with distinct skin morphology and histopathological findings. Tuberculosis verrucosa cutis is a classification form of cutaneous tuberculosis, a paucibacillary disease caused by exogenous reinfection (inoculation) after sensitization on an immunocompromised individual. Management of cutaneous TB is generally similar to the management of pulmonary TB. In this paper, we presented a case of 62 years old male with a purplish rough patch on the right dorsum of the foot that began 1,5 years. There is a history of trauma and pulmonary TB. On the right dorsum of the foot, we identified a verrucous lesion with a livid base, irregular with the size of 6x3 cm. The diagnosis is established based on clinical features and histopathology evaluation. The patient received treatment with antituberculosis medication, topical mupirocin ointment 2%, and vaseline petroleum jelly. Improvement of the lesions was observed after the patient completed the intensive phase in two months of treatment with antituberculosis drugs.
{"title":"Tuberculosis verrucosa cutis in a patient with pulmonary tuberculosis: A case report","authors":"Mimi Maulida, Nanda Earlia, Vella Vella, Karamina Maghfirah","doi":"10.20885/jkki.vol14.iss2.art13","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art13","url":null,"abstract":"Cutaneous tuberculosis is a skin disorder caused by Mycobacterium tuberculosis, commonly classified according to its spreading route into endogenous, exogenous, and hematogenous, with distinct skin morphology and histopathological findings. Tuberculosis verrucosa cutis is a classification form of cutaneous tuberculosis, a paucibacillary disease caused by exogenous reinfection (inoculation) after sensitization on an immunocompromised individual. Management of cutaneous TB is generally similar to the management of pulmonary TB. In this paper, we presented a case of 62 years old male with a purplish rough patch on the right dorsum of the foot that began 1,5 years. There is a history of trauma and pulmonary TB. On the right dorsum of the foot, we identified a verrucous lesion with a livid base, irregular with the size of 6x3 cm. The diagnosis is established based on clinical features and histopathology evaluation. The patient received treatment with antituberculosis medication, topical mupirocin ointment 2%, and vaseline petroleum jelly. Improvement of the lesions was observed after the patient completed the intensive phase in two months of treatment with antituberculosis drugs.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.20885/jkki.vol14.iss2.art6
Danu Darmawan, Endang Sri Lestari, Sigit Adi Prasetyo, M. Muniroh, Agung Aji Prasetyo
Background: Cholestasis is a disorder of the formation or flow of bile. Among its contributors, tumour necrosis factor (TNF)-α stands out as the most influential inducer of apoptosis. Meanwhile, ursodeoxycholic acid (UDCA) is a valuable agent with choleretic properties, protecting the hepatobiliary system. Glutathione (GSH) enhances endothelial response and prevents liver fibrosisObjectives: This study evaluates the effect of a combination of GSH-UDCA on splenic TNF-α expression and apoptosis index in Sprague Dawley (SD) rats with cholestasisMethods: This experiment with a post-tests-only control group design involving 28 male SD rats. They were randomly into four groups: group (K) with 20 mg UDCA, group 1 (P1) with 10 mg UDCA + 10 mg GSH, group 2 (P2) was given UDCA 20 mg + GSH 15 mg, and group 3 (P3) was given UDCA 30 mg + GSH 20 mg. Cholestasis was obtained by ligation of the common bile duct through a laparotomy. During three weeks of trial, rats were administered daily with UDCA orally and GSH intramuscularly. On day 22, rats were sacrificed and spleen samples were taken for anatomical pathology examination. Results: There were significant differences in TNF-α expression between groups K vs P3; P1 vs P3, and P2 vs P3 (p=0.002). There was a significant difference in the apoptotic index between groups K vs P1 (p<0.001); K vs P2 (p=0.004), and K vs P3 (p=0.005).Conclusions: The UDCA-GSH combination demonstrated a prophylactic effect in SD rats with cholestasis and might be an effective supplemental therapy with UDCA for cholestatic diseases. The difference in TNF-α expression and apoptotic index was lower in SD rats UDCA-glutathione combination group than single dose UDCA. Between TNF-α and the apoptotic index, there is a moderate positive relation.
{"title":"Effects of ursodeoxycholic acid and glutathione combination in spleen TNF-α and apoptotic index in rats with cholestasis","authors":"Danu Darmawan, Endang Sri Lestari, Sigit Adi Prasetyo, M. Muniroh, Agung Aji Prasetyo","doi":"10.20885/jkki.vol14.iss2.art6","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art6","url":null,"abstract":"Background: Cholestasis is a disorder of the formation or flow of bile. Among its contributors, tumour necrosis factor (TNF)-α stands out as the most influential inducer of apoptosis. Meanwhile, ursodeoxycholic acid (UDCA) is a valuable agent with choleretic properties, protecting the hepatobiliary system. Glutathione (GSH) enhances endothelial response and prevents liver fibrosisObjectives: This study evaluates the effect of a combination of GSH-UDCA on splenic TNF-α expression and apoptosis index in Sprague Dawley (SD) rats with cholestasisMethods: This experiment with a post-tests-only control group design involving 28 male SD rats. They were randomly into four groups: group (K) with 20 mg UDCA, group 1 (P1) with 10 mg UDCA + 10 mg GSH, group 2 (P2) was given UDCA 20 mg + GSH 15 mg, and group 3 (P3) was given UDCA 30 mg + GSH 20 mg. Cholestasis was obtained by ligation of the common bile duct through a laparotomy. During three weeks of trial, rats were administered daily with UDCA orally and GSH intramuscularly. On day 22, rats were sacrificed and spleen samples were taken for anatomical pathology examination. Results: There were significant differences in TNF-α expression between groups K vs P3; P1 vs P3, and P2 vs P3 (p=0.002). There was a significant difference in the apoptotic index between groups K vs P1 (p<0.001); K vs P2 (p=0.004), and K vs P3 (p=0.005).Conclusions: The UDCA-GSH combination demonstrated a prophylactic effect in SD rats with cholestasis and might be an effective supplemental therapy with UDCA for cholestatic diseases. The difference in TNF-α expression and apoptotic index was lower in SD rats UDCA-glutathione combination group than single dose UDCA. Between TNF-α and the apoptotic index, there is a moderate positive relation.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.20885/jkki.vol14.iss2.art4
Tinalda Nur Amelia Wijaya, Meiyanti Meiyanti
Background: Indonesia is currently contributing to the third highest incidence of leprosy in the world. Leprosy sufferers who do not undergo regular treatment have risk in transmitting the disease to others. Moreover, it may also cause disability problems and social impacts. Objective: This study aims to analyse relationships between drug side effects, subject characteristics and adherence to treatment of leprosy patients.Method: This was an observational analytic study with a cross-sectional research design with a sample of 115 leprosy patients. This study was conducted at four Puskesmas (Community Health Centre) of Sukasari sub-district, Subang from October to December 2021. Its data was collected by using a questionnaire consisting of patient characteristics; the MMAS-8 questionnaire was used to assess levels of the adherence and the side effects. Then the data were analysed by Chi-Square and Fisher's Exact test, with a significance level of 0.05.Results: The results of this study revealed that 60 (52.2%) of the subjects were categorized in the medication adherence with a score of 0 and 22 (19.1%) of them were relatively high adherence with a score of 1-2. Most of the 93.9% of subjects experienced drug side effects while undergoing the leprosy treatment. There was no significant relationship between drug side effects (p=0.442), genders (p=0.848), ages (p=0.275), education levels (p=0.134), types of occupations (p=0.847) and adherence to treatment of leprosy patients. Conclusion: Several factors such as drug side effects, ages, genders, education levels and occupations were not significantly related to medication adherence in the leprosy patients.
{"title":"Analyzing factors of leprosy patients’ adherence to treatment in Sukasari Sub-district, Subang","authors":"Tinalda Nur Amelia Wijaya, Meiyanti Meiyanti","doi":"10.20885/jkki.vol14.iss2.art4","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art4","url":null,"abstract":"Background: Indonesia is currently contributing to the third highest incidence of leprosy in the world. Leprosy sufferers who do not undergo regular treatment have risk in transmitting the disease to others. Moreover, it may also cause disability problems and social impacts. Objective: This study aims to analyse relationships between drug side effects, subject characteristics and adherence to treatment of leprosy patients.Method: This was an observational analytic study with a cross-sectional research design with a sample of 115 leprosy patients. This study was conducted at four Puskesmas (Community Health Centre) of Sukasari sub-district, Subang from October to December 2021. Its data was collected by using a questionnaire consisting of patient characteristics; the MMAS-8 questionnaire was used to assess levels of the adherence and the side effects. Then the data were analysed by Chi-Square and Fisher's Exact test, with a significance level of 0.05.Results: The results of this study revealed that 60 (52.2%) of the subjects were categorized in the medication adherence with a score of 0 and 22 (19.1%) of them were relatively high adherence with a score of 1-2. Most of the 93.9% of subjects experienced drug side effects while undergoing the leprosy treatment. There was no significant relationship between drug side effects (p=0.442), genders (p=0.848), ages (p=0.275), education levels (p=0.134), types of occupations (p=0.847) and adherence to treatment of leprosy patients. Conclusion: Several factors such as drug side effects, ages, genders, education levels and occupations were not significantly related to medication adherence in the leprosy patients.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.20885/jkki.vol14.iss2.art12
T. Triyono, U. Sukorini, Andri Christianalusi, Sri Mulatsih, Hans Vrielink
Covid-19 Convalescent Plasma (CCP) was one of alternative therapy for Covid-19 patients. The use of convalescent plasma as an adjunct therapy must consider its efficacy, benefits, and risk, both for the patient and the donor. Convalescent Plasma was produced from purified human plasma. Convalescent Plasma was a passive immunity which provide active antibodies. CCP give immunity to the patient through the transfusion of a survivor’s antibodies. We were reporting a case of male CCP donor with long existence of SARS-Cov-2. This donor performed up to 16 donations, which was an unusual case in CCP donor. The SARS-CoV-2 antibody titer was analyzed using Elecsys® Anti-SARS-CoV-2 (Roche Diagnostics). The SARS-CoV-2 antibody showed high level of total SARS-CoV-2 antibodies, with the Cut off Indexes (COI) anti SARS-Cov-2 antibody were still quite high (132 U/mL). The highest donor antibody COI (204.6 U/mL) was seen in 11th donation at 6 months after recovery, while the lowest antibody COI (130.8 U/mL) was in 15th donation at 7 months after recovering. Evaluation of donor health status were performed after the 16th donation and analyzed as well. In conclusion, 16 CCP donations of 600 mL resulting in 48 units of 200 mL plasma in 8 months was safe for the donor, with the SARS-Cov-2 antibody titer remained high in the whole period.
{"title":"A male Covid-19 convalescent plasma donor with long existence of SARS-Cov-2 antibodies: a case report","authors":"T. Triyono, U. Sukorini, Andri Christianalusi, Sri Mulatsih, Hans Vrielink","doi":"10.20885/jkki.vol14.iss2.art12","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art12","url":null,"abstract":"Covid-19 Convalescent Plasma (CCP) was one of alternative therapy for Covid-19 patients. The use of convalescent plasma as an adjunct therapy must consider its efficacy, benefits, and risk, both for the patient and the donor. Convalescent Plasma was produced from purified human plasma. Convalescent Plasma was a passive immunity which provide active antibodies. CCP give immunity to the patient through the transfusion of a survivor’s antibodies. We were reporting a case of male CCP donor with long existence of SARS-Cov-2. This donor performed up to 16 donations, which was an unusual case in CCP donor. The SARS-CoV-2 antibody titer was analyzed using Elecsys® Anti-SARS-CoV-2 (Roche Diagnostics). The SARS-CoV-2 antibody showed high level of total SARS-CoV-2 antibodies, with the Cut off Indexes (COI) anti SARS-Cov-2 antibody were still quite high (132 U/mL). The highest donor antibody COI (204.6 U/mL) was seen in 11th donation at 6 months after recovery, while the lowest antibody COI (130.8 U/mL) was in 15th donation at 7 months after recovering. Evaluation of donor health status were performed after the 16th donation and analyzed as well. In conclusion, 16 CCP donations of 600 mL resulting in 48 units of 200 mL plasma in 8 months was safe for the donor, with the SARS-Cov-2 antibody titer remained high in the whole period.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.20885/jkki.vol14.iss2.art9
A. Handaya, D. S. Heriyanto, H. Susanto, Yudi Susanto, Kamal Agung Yudayana, Ida Ayu Setyawati Sri Krisna Dewi, Aditya Rifqi Fauzi, J. Andrew, Kevin Radinal, Azriel F K Aditya
Background: Colorectal cancer (CRC) is the third highest-ranked cancer and causes high mortality in patients with a low survival rate. The lack of sensitivity and specificity of clinical and other diagnostic modalities results in a higher mortality rate. Therefore, the exploration of potential early biomarkers for CRCs is necessary.Objective: We aimed to evaluate the local expressions of potential tumor suppressor and oncogenic miRNAs in CRC patients in Indonesia. Methods: This retrospective cohort study involving thirty-one colorectal carcinoma patients at Dr. Sardjito Hospital Yogyakarta from January 2014-December 2017. Total RNA was isolated, and the expressions of miR-21, miR-92a, miR-96, miR-26b, miR144, and miR-195 were measured by real-time quantitative PCR. The correlation between miRNAs and other predictors was determined by Spearman correlation, and the association of miRNA expression and other clinical parameters used logistic regression.Results: The local expression of miR-195 decreased significantly in the tumor sites. In contrast, miR-21 activity tends to increase in the local tumor. Meanwhile, the expressions for miR-92a, miR-96, miR26b, and miR-144 in the same subjects were non-significant. MiR-195 was also significantly associated to cancer stage (r=-0.570, p=0.001) with significant odds ratio (OR=0.892, 95% CI=0.804–0.990, p=0.031).Conclusion: Our study was the first to report aberrant expressions of miRNA-21, miRNA-195, miRNA-92a, miRNA-26b, miRNA-96, and miRNA-144 in Indonesian CRC patients. The tumor suppressor miRNA-195 expression was superior among others to serve as an early biomarker in detecting and predicting CRC disease progression.
{"title":"Detecting miRNAs expression as the early prognostic factor for patients with colorectal cancer in Dr. Sardjito Hospital Yogyakarta : A preliminary study","authors":"A. Handaya, D. S. Heriyanto, H. Susanto, Yudi Susanto, Kamal Agung Yudayana, Ida Ayu Setyawati Sri Krisna Dewi, Aditya Rifqi Fauzi, J. Andrew, Kevin Radinal, Azriel F K Aditya","doi":"10.20885/jkki.vol14.iss2.art9","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art9","url":null,"abstract":"Background: Colorectal cancer (CRC) is the third highest-ranked cancer and causes high mortality in patients with a low survival rate. The lack of sensitivity and specificity of clinical and other diagnostic modalities results in a higher mortality rate. Therefore, the exploration of potential early biomarkers for CRCs is necessary.Objective: We aimed to evaluate the local expressions of potential tumor suppressor and oncogenic miRNAs in CRC patients in Indonesia. Methods: This retrospective cohort study involving thirty-one colorectal carcinoma patients at Dr. Sardjito Hospital Yogyakarta from January 2014-December 2017. Total RNA was isolated, and the expressions of miR-21, miR-92a, miR-96, miR-26b, miR144, and miR-195 were measured by real-time quantitative PCR. The correlation between miRNAs and other predictors was determined by Spearman correlation, and the association of miRNA expression and other clinical parameters used logistic regression.Results: The local expression of miR-195 decreased significantly in the tumor sites. In contrast, miR-21 activity tends to increase in the local tumor. Meanwhile, the expressions for miR-92a, miR-96, miR26b, and miR-144 in the same subjects were non-significant. MiR-195 was also significantly associated to cancer stage (r=-0.570, p=0.001) with significant odds ratio (OR=0.892, 95% CI=0.804–0.990, p=0.031).Conclusion: Our study was the first to report aberrant expressions of miRNA-21, miRNA-195, miRNA-92a, miRNA-26b, miRNA-96, and miRNA-144 in Indonesian CRC patients. The tumor suppressor miRNA-195 expression was superior among others to serve as an early biomarker in detecting and predicting CRC disease progression.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.20885/jkki.vol14.iss2.art3
Silvani Permatasari, Syarpin, D. Pujianto
Background: Saluang belum (Lavanga sarmentosa) is one of Kalimantan's typical plants, which is as efficacious as a traditional medicine to increase sexual activity and male fertility. Based on previous studies, the content of flavonoid and steroid in 70% ethanol extract of L. sarmentosa were able to affect sperm quality of mice. Studies related to L. sarmentosa are still limited for phytochemical test and their bioactivity on human spermatozoa motility in vitro.Objective: This study is to perform phytochemical tests of compound content in fractionation with eluents of high and low polarity, namely methanol and chloroform, and then to test their bioactivity on the motility of human spermatozoa in vitro.Methods: L. sarmentosa was extracted with 96% ethanol and fractionated by using a vacuum chromatography column with chloroform and methanol as the eluent. Then obtained samples were analysed by a quantitative phytochemical test. The samples used in-vitro human spermatozoa were divided into eleven groups: control group, group administered with L. sarmentosa extract eluent chloroform of 10, 50, 100, 500, and 1000 ng/mL, and same concentration with extract eluent methanol. Furthermore, the sperm motility was analysed by using a Computer Assisted Sperm Analyser (CASA). Results: The methanol and chloroform fraction of L. sarmentosa root extract contained metabolites, namely terpenoids, flavonoids, steroids, alkaloids, saponins, and tannins. The sperm motility increased significantly at the treatment group of the methanol and chloroform fractions compared to the control group. There was a significant difference between the sperm motility incubated with methanol and that with chloroform fraction at concentration 500 and 1000 ng/mL.Conclusion: The results of sperm motility were higher in the methanol fraction than those in the chloroform fraction.
{"title":"Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility","authors":"Silvani Permatasari, Syarpin, D. Pujianto","doi":"10.20885/jkki.vol14.iss2.art3","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art3","url":null,"abstract":"Background: Saluang belum (Lavanga sarmentosa) is one of Kalimantan's typical plants, which is as efficacious as a traditional medicine to increase sexual activity and male fertility. Based on previous studies, the content of flavonoid and steroid in 70% ethanol extract of L. sarmentosa were able to affect sperm quality of mice. Studies related to L. sarmentosa are still limited for phytochemical test and their bioactivity on human spermatozoa motility in vitro.Objective: This study is to perform phytochemical tests of compound content in fractionation with eluents of high and low polarity, namely methanol and chloroform, and then to test their bioactivity on the motility of human spermatozoa in vitro.Methods: L. sarmentosa was extracted with 96% ethanol and fractionated by using a vacuum chromatography column with chloroform and methanol as the eluent. Then obtained samples were analysed by a quantitative phytochemical test. The samples used in-vitro human spermatozoa were divided into eleven groups: control group, group administered with L. sarmentosa extract eluent chloroform of 10, 50, 100, 500, and 1000 ng/mL, and same concentration with extract eluent methanol. Furthermore, the sperm motility was analysed by using a Computer Assisted Sperm Analyser (CASA). Results: The methanol and chloroform fraction of L. sarmentosa root extract contained metabolites, namely terpenoids, flavonoids, steroids, alkaloids, saponins, and tannins. The sperm motility increased significantly at the treatment group of the methanol and chloroform fractions compared to the control group. There was a significant difference between the sperm motility incubated with methanol and that with chloroform fraction at concentration 500 and 1000 ng/mL.Conclusion: The results of sperm motility were higher in the methanol fraction than those in the chloroform fraction.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.20885/jkki.vol14.iss2.art2
Uly Astuti Siregar Uly Astuti Siregar, May Valzon, Fitrianti, Arif Budianto
Background: Indonesia ranks third in the world regarding air pollution due to forest and land fires; most of the land burned is a peatland. Particulate matter (PM) 2.5 is the largest component of the total smoke particles. Short-term and long-term exposure to PM2.5 remains a hazard to human health.Objective: This study aims to examine the effect of exposure to peat biomass smoke on serum malondialdehyde (MDA) levels and body weight of Wistar rats.Methods: Experimental animals were randomly divided into three groups: Control group (C) is not given treatment, and treatment groups (X1 and X2) are exposed to smoke from peat biomass of 100 g and 150 g of biomass for 60 seconds per day for 14 days. The body weight was examined before and after treatment, while Serum MDA levels were examined after treatment.Results: The results showed significant differences (p < 0.05) in serum MDA levels between groups. The highest serum MDA levels were found in group X2 (3.03 ± 0.185 nmol/ml), followed by group X1 (2.67 ± 0.212 nmol/ml) compared to the control group (2.24 ± 0.476 nmol/ml). In contrast, increasing body weight between groups did not show a significant difference.Conclusion: Exposure to PM 2.5 from peat biomass smoke increases oxidative stress in experimental animals.
{"title":"Effect of peat biomass smoke exposure on oxidative stress in Wistar rats","authors":"Uly Astuti Siregar Uly Astuti Siregar, May Valzon, Fitrianti, Arif Budianto","doi":"10.20885/jkki.vol14.iss2.art2","DOIUrl":"https://doi.org/10.20885/jkki.vol14.iss2.art2","url":null,"abstract":"Background: Indonesia ranks third in the world regarding air pollution due to forest and land fires; most of the land burned is a peatland. Particulate matter (PM) 2.5 is the largest component of the total smoke particles. Short-term and long-term exposure to PM2.5 remains a hazard to human health.Objective: This study aims to examine the effect of exposure to peat biomass smoke on serum malondialdehyde (MDA) levels and body weight of Wistar rats.Methods: Experimental animals were randomly divided into three groups: Control group (C) is not given treatment, and treatment groups (X1 and X2) are exposed to smoke from peat biomass of 100 g and 150 g of biomass for 60 seconds per day for 14 days. The body weight was examined before and after treatment, while Serum MDA levels were examined after treatment.Results: The results showed significant differences (p < 0.05) in serum MDA levels between groups. The highest serum MDA levels were found in group X2 (3.03 ± 0.185 nmol/ml), followed by group X1 (2.67 ± 0.212 nmol/ml) compared to the control group (2.24 ± 0.476 nmol/ml). In contrast, increasing body weight between groups did not show a significant difference.Conclusion: Exposure to PM 2.5 from peat biomass smoke increases oxidative stress in experimental animals.","PeriodicalId":508182,"journal":{"name":"Jurnal Kedokteran dan Kesehatan Indonesia","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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