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The Association between Dietary Intake and Lifestyle Patterns of People with Type 2 Diabetes Mellitus in Manipur, India 印度曼尼普尔 2 型糖尿病患者的膳食摄入量与生活方式之间的关系
Pub Date : 2024-05-10 DOI: 10.9734/ejnfs/2024/v16i61434
Mongjam Priyangka Chanu, Namita Singh
The aim of the study was to examine the association between dietary intake and lifestyle pattern of diabetes patients in Manipur. Tribal and non tribal respondents between 45-64 years of age were randomly selected from the Regional Institute of Medical Sciences (RIMS) Hospital Manipur. Total number of 200 study subjects, 100 tribal and 100 non tribal subjects constituted the study. The information was collected on socio-demographic profile, clinical, anthropometric measurements and dietary intakes of the patients by using interview schedule and 24-hour dietary recall methods. The data were analyzed and tabulated using statistical tools such as frequency, percentage, mean, standard deviation and Pearson’s correlation test. The key results indicate that alcohol consumption, elevated triglycerides levels, low energy, oils and sugar intake were notably linked with the prevalence of diabetes. Additionally, low calcium intake, particularly among non tribal individuals, demonstrated a significant correlation with elevated Fasting Blood Glucose (FBG) levels, suggesting a potential risk factor for Type 2 diabetes. Moreover, low calcium intake among the non-tribal community was associated with increased Post Prandial (PP) test results, indicating a rise in post-meal glucose levels. Notably, tribal energy intake and non-tribal protein consumption were significantly associated with HbA1c levels, reflecting their impact on glycemic control.
本研究旨在探讨曼尼普尔邦糖尿病患者的饮食摄入与生活方式之间的关系。研究人员从曼尼普尔地区医学科学研究所(RIMS)医院随机抽取了 45-64 岁的部落和非部落受访者。研究对象共 200 人,其中 100 人为部落成员,100 人为非部落成员。通过访谈表和 24 小时饮食回忆法,收集了患者的社会人口概况、临床、人体测量和饮食摄入量等信息。使用频率、百分比、平均值、标准偏差和皮尔逊相关性检验等统计工具对数据进行了分析和制表。主要结果表明,饮酒、甘油三酯水平升高、能量、油脂和糖的摄入量低与糖尿病的发病率有明显的联系。此外,钙摄入量低,尤其是非部落居民的钙摄入量低,与空腹血糖(FBG)水平升高有显著相关性,表明这是 2 型糖尿病的潜在风险因素。此外,非部落居民钙摄入量低与餐后血糖(PP)测试结果升高有关,表明餐后血糖水平升高。值得注意的是,部落的能量摄入量和非部落的蛋白质消耗量与 HbA1c 水平显著相关,反映出它们对血糖控制的影响。
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引用次数: 0
Extraction of Natural Food Color from Tomato, Carrot and Turmeric 从番茄、胡萝卜和姜黄中提取天然食用色素
Pub Date : 2024-05-09 DOI: 10.9734/ejnfs/2024/v16i51433
F. N. Kabir, Shohel Rana Palleb, Md. Abdul Halim, Sourav Biswas Nayan, M. B. Uddin, Mohammad Gulzarul Aziz
The demand for natural and healthier food products has increased dramatically in recent years due to growing consumer awareness of the impact food has on health as well as evidence of adverse effects from various ingredients, including some additives. This study evaluates the extraction method, solvent effects, precipitation, identification, purification, and lycopene, carotene, and curcumin content from turmeric, carrot, and tomato. Along-side, tomato, carrot, and turmeric samples were subjected to two different extraction and purification processes: solvent extraction for lycopene and carotene, and alkalization for curcumin. The samples were extracted and purified at room temperature (30ºC) and chilled (4ºC) for a period of three weeks. Tomato extracts had lycopene contents ranging from 0.0153 to 0.0362 mg/100g. obtained carotene content, which was then extracted using a solvent in the range of 61.43 to 81.72 mg/100g. The alkalization process produced a curcumin concentration of 91.17 to 110.41 mg/100g. Comparing the ethyl acetate extraction technique to the anti-solvent method, a greater amount of lycopene (red) and carotene (orange) precipitation was obtained. The maximum amount of curcumin precipitation obtained in lower pH solution. Lower pH is important to maintain the stability of curcumin precipitation. The experience of this research work suggested that lycopene from tomatoes, carotene from carrots, and curcumin from turmeric might be an excellent source to meet the increasing need for natural colorants.
近年来,由于消费者越来越意识到食品对健康的影响,以及有证据表明各种成分(包括一些添加剂)会产生不良影响,因此对天然健康食品的需求急剧增加。本研究评估了姜黄、胡萝卜和番茄的提取方法、溶剂作用、沉淀、鉴定、纯化以及番茄红素、胡萝卜素和姜黄素的含量。同时,对番茄、胡萝卜和姜黄样品进行了两种不同的提取和纯化过程:溶剂提取番茄红素和胡萝卜素,碱化姜黄素。样品分别在室温(30ºC)和冷藏(4ºC)条件下进行萃取和纯化,为期三周。番茄提取物中番茄红素的含量为 0.0153 至 0.0362 毫克/100 克,胡萝卜素的含量为 61.43 至 81.72 毫克/100 克。碱化过程产生的姜黄素浓度为 91.17 至 110.41 毫克/100 克。乙酸乙酯萃取法与反溶剂萃取法相比,获得了更多的番茄红素(红色)和胡萝卜素(橙色)沉淀。在 pH 值较低的溶液中,姜黄素析出量最大。较低的 pH 值对保持姜黄素沉淀的稳定性非常重要。这项研究工作的经验表明,番茄中的番茄红素、胡萝卜中的胡萝卜素和姜黄中的姜黄素可能是满足日益增长的天然着色剂需求的极好来源。
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引用次数: 0
Polycyclic Aromatic Hydrocarbons Contamination and Quality of Locally Produced Crude Palm Oil in South-South Zone, Nigeria 尼日利亚南部地区当地生产的棕榈油原油的多环芳烃污染和质量
Pub Date : 2024-05-08 DOI: 10.9734/ejnfs/2024/v16i51432
Onyegeme-Okerenta M. Blessing, Isiodu C. Vera, Essien B. Eka
Background: Oil palm, Elaeis guineensis Jacq is a perennial crop mainly cultivated for its vegetable oil. The fatty acid composition of crude palm oil (CPO) is mostly composed of palmitic, stearic oleic, and linoleic acids.Objectives: This study evaluated the quality of locally produced CPO from six states of South-South zone, Nigeria.Methods: The parameters of CPOs were characterized by specific gravity (SG), acid value (AV), percentage of free fatty acid (%FFA), and saponification value (SV) and peroxide value (PV). Polycyclic aromatic hydrocarbons (PAHs) in CPO were quantified using standard procedures, the related risk due to daily consumption of 25 g was characterized by lifetime cancer risk (LCR) and margin of exposure (MOE).Results: The study observed that the mean values of the parameters were 0.931 SG; 5.01 mgKOH g-1 AV; 2.28% FFA; 177.27 mgKOH g-1 SV and 29.65 meqO2 kg-1 PV. Apart from %FFA, the others did not meet the requirements recommended by the Codex Alimentarius Commission of the joint WHO/FAO. Diagnostic ratios of PAHs suggested a mixture of petrogenic and pyrogenic origins. The MOEs showed low health concern while 16PAH-LCR values which ranged from 2.48E-04 - 4.48E-04 were unacceptable. The screening value 0.003 was significantly (p> 0.05) lower than the computed total BaP equivalent (BaPeq) values indicating potential human health concerns. It is recommended that CPO processing and storage methods should be properly monitored to prevent quality reduction and contamination, which might have adverse effect on consumers.
背景:油棕(Elaeis guineensis Jacq)是一种多年生作物,主要用于生产植物油。毛棕榈油(CPO)的脂肪酸组成主要是棕榈酸、硬脂酸、油酸和亚油酸:本研究评估了尼日利亚南部六个州当地生产的 CPO 的质量:方法:通过比重(SG)、酸值(AV)、游离脂肪酸百分比(%FFA)、皂化值(SV)和过氧化值(PV)对氯化石蜡的参数进行表征。采用标准程序对氯化石蜡中的多环芳烃(PAHs)进行了量化,并以终生癌症风险(LCR)和暴露限值(MOE)来表征每天食用 25 克氯化石蜡的相关风险:研究发现,这些参数的平均值为:0.931 SG;5.01 mgKOH g-1 AV;2.28% FFA;177.27 mgKOH g-1 SV 和 29.65 meqO2 kg-1 PV。除 FFA 百分比外,其他指标均未达到世界卫生组织/粮农组织联合食品法典委员会建议的要求。多环芳烃的诊断比率表明,石油来源和热源来源混合在一起。暴露限值对健康的影响较小,而 16PAH-LCR 值介于 2.48E-04 - 4.48E-04 之间,令人无法接受。筛选值 0.003 明显(p> 0.05)低于计算得出的总 BaP 当量 (BaPeq),表明可能存在人类健康问题。建议适当监测氯化石蜡的加工和储存方法,以防止质量下降和污染,从而对消费者造成不利影响。
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引用次数: 0
Analysing the Composition of Commercial Turmeric Powder: Assessing Contaminants and Its Impacts Curcumin and Water-soluble Vitamins Levels 分析商用姜黄粉的成分:评估污染物及其对姜黄素和水溶性维生素含量的影响
Pub Date : 2024-05-08 DOI: 10.9734/ejnfs/2024/v16i51431
Seth Ankamah, Christopher Larbie, Marina Tandoh, Kate Nana Ama Afram, Godwin Agbeka
Aims: Turmeric, with its active component curcumin, has garnered global attention for its medicinal benefits, including anti-inflammatory and antioxidant properties. This study aimed to analyse turmeric powder obtained from the Greater Accra Metropolis for nutrients and contaminants.Study Design: Experimental.Place and Duration of Study: Entrance Pharmaceuticals, Accra for 6 months.Methodology: 22 samples from 10 different processing sites and open markets were tested using physical and chemical methods. HPLC identified curcumin, ascorbic acid, riboflavin, thiamine, and pyridoxine levels. An independent t-test was done to compare concentrations of these nutrients in the powdered turmeric samples from the two sources.Results: Assessment showed no yellow lead salts but 9.1% were adulterated with chalk, and 91% contained metanil yellow. Curcumin (2014.95 vs. 567.79), riboflavin (21.60 vs. 1.75), thiamine (14.75 vs. 0.65 mg/mL), pyridoxine (9.35 vs. 0.65 mg/mL), and ascorbic acid (0.00 vs. 101.60 mg/mL) were significantly higher (p<0.05) in processed samples than open market ones. Samples without adulterants had higher curcumin and micronutrient levels.Conclusion: Strengthening monitoring programs is crucial to tackling food adulteration concerns.
研究目的:姜黄及其活性成分姜黄素因具有抗炎和抗氧化等药用功效而备受全球关注。本研究旨在分析从大阿克拉大都市获得的姜黄粉的营养成分和污染物:实验:研究方法:使用物理和化学方法对来自 10 个不同加工点和公开市场的 22 个样本进行了检测。高效液相色谱法确定了姜黄素、抗坏血酸、核黄素、硫胺素和吡哆醇的含量。对两种来源的姜黄粉末样本中这些营养素的浓度进行了独立的 t 检验:评估结果表明,没有发现黄铅盐,但有 9.1%掺入了白垩,91%含有偏苯胺黄。姜黄素(2014.95 对 567.79)、核黄素(21.60 对 1.75)、硫胺素(14.75 对 0.65 毫克/毫升)、吡哆醇(9.35 对 0.65 毫克/毫升)和抗坏血酸(0.00 对 101.60 毫克/毫升)在加工样品中的含量显著高于公开市场样品(p<0.05)。没有掺假的样品中姜黄素和微量营养素的含量更高:结论:加强监测计划对于解决食品掺假问题至关重要。
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引用次数: 0
Optimization and Shelf-life Study of Finger Millet-based Cookies Premix 基于手指小米的饼干预混料的优化和保质期研究
Pub Date : 2024-05-07 DOI: 10.9734/ejnfs/2024/v16i51430
Sujata Sethy, R. Mogra
Multigrain cookie premixes were developed using finger millet (Eleusine coracana) to provide a nutritious food option for vulnerable groups, especially preschool children facing micronutrient malnutrition. Three types of cookie premixes were formulated with varying proportions of grains and evaluated for sensory parameters using a 9-point hedonic scale. The best combination of calcium and iron-rich ingredient s was found to be finger millet flour, refined wheat flour, grain amaranth, garden cress seed, milk powder in the ratio of 40:25:20:5:10. This premix was also evaluated for nutritional composition and shelf life. The cookies made from this premix were rich in essential nutrients, with 319.8 mg of calcium and 8.89 mg of iron per 100 g. The sensory evaluation showed that all the premixes were well accepted (sensory score >7) and the selected premix remained acceptable over a storage period of 90 days.
为了给弱势群体,尤其是面临微量营养素营养不良问题的学龄前儿童提供一种营养丰富的食品选择,我们利用小米(Eleusine coracana)开发了多谷物饼干预混料。研究人员用不同比例的谷物配制了三种饼干预混料,并使用 9 点享乐量表对感官参数进行了评估。结果发现,富含钙和铁的配料的最佳组合是小米粉、精制小麦粉、苋菜、芹菜籽和奶粉,比例为 40:25:20:5:10。还对这种预混料的营养成分和保质期进行了评估。感官评价显示,所有预混料的接受度都很好(感官评分大于 7 分),所选预混料在 90 天的储存期内仍可接受。
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引用次数: 0
Search for Local Food Supplements: Study of the Nutritional Value of 15 Plant Species from Niger 寻找当地食品补充剂:尼日尔 15 种植物的营养价值研究
Pub Date : 2024-05-06 DOI: 10.9734/ejnfs/2024/v16i51427
Soumana Sourounda Idé, Mahamane Sabiou Sani Maazou, S. Sangaré, Maman Mahaman Salissou, Almoustapha Théodore Yatta, Mahamane Djika Hachimou, Garba dit Gado Halidou Ousseini, Almou Abdoulaye Alio, Sabo Haoua Seini, Ameyapoh Yaovi
In Niger, the prevalence of malnutrition and mortality linked to undernutrition among Nigerien children under 5 years of age remains high and constitutes a major concern for public authorities. However, the country is teeming with significant biodiversity that could help combat high levels of undernutrition among children aged 6 to 59 months. The present study aimed to evaluate the nutritional quality of 15 plant species of which 17 samples were taken as potential complementary foods. The water, ash, lipids, protein and cellulose content were determined. Carbohydrate content and energy density were calculated. Biochemical characterization of the sampled foods showed that Glycine max seeds were richer in protein (36.39%). Neocarya macrophylla almond has a relatively high lipid content (60.75%) and cellulose content (26.74%). The pulp of Hyphaene thebaica was rich in mineral elements (7.16%). The tuber of Ipomoea batatas (95.25%) and the pulp of Neocarya macrophylla (93.01%) contained sufficient carbohydrates, and the humidity was found to be higher specifically in the cereal (Pennisetum glaucum) and the tuber of Borassus aethiopum with rates of 6.72% and 5.70 %, respectively. In addition, a positive and significant correlation was observed on the one hand between the cellulose content and that of proteins and lipids and between the protein and lipid levels. In contrast, the correlation between carbohydrate levels and those of proteins and lipids was negative and very significant. The results confirm that pulps and kernels of fruits, legumes, tubers, and cereals can be incorporated into numerous preparations intended for children to supplement their diet.
在尼日尔,5 岁以下儿童的营养不良率和与营养不良相关的死亡率居高不下,成为公共当局关注的主要问题。然而,尼日尔拥有丰富的生物多样性,可以帮助解决 6 至 59 个月儿童营养不良的问题。本研究旨在评估 15 种植物的营养质量,其中 17 个样本被作为潜在的补充食品。测定了水分、灰分、脂类、蛋白质和纤维素的含量。计算了碳水化合物含量和能量密度。样本食物的生化特征显示,最大甘氨酸种子的蛋白质含量较高(36.39%)。Neocarya macrophylla 杏仁的脂质含量(60.75%)和纤维素含量(26.74%)相对较高。Hyphaene thebaica 的果肉含有丰富的矿物质元素(7.16%)。Ipomoea batatas 的块茎(95.25%)和 Neocarya macrophylla 的果肉(93.01%)含有充足的碳水化合物,湿度较高的是谷物(Pennisetum glaucum)和 Borassus aethiopum 的块茎,分别为 6.72% 和 5.70%。此外,一方面,纤维素含量与蛋白质和脂质含量之间以及蛋白质和脂质含量之间存在显著的正相关关系。相反,碳水化合物含量与蛋白质和脂质含量之间的相关性为负,且非常显著。研究结果证实,水果、豆类、块茎和谷物的果肉和果核可以添加到多种供儿童补充营养的制剂中。
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引用次数: 0
Evaluation of Nutrient Balance and Nutrition use Efficiency on Red Gram (Cajanus cajan) under Various Foliar Feeding 不同叶面施肥条件下红禾秆(Cajanus cajan)的营养平衡和营养利用效率评估
Pub Date : 2024-05-06 DOI: 10.9734/ejnfs/2024/v16i51429
Akshay Kumar G, Udhaya Kumar K, Vanathi D, Samundeshwari R, Silambarasan M, Patricia Kalairasi J
AIM: To assess the nutrient balance and nutrient use efficiency on red gram.Place and Duration of Study: A field experiment was carried out during Rabi season 2023-24 at Instruction farm of Karunya Institute of Technology and Sciences, Coimbatore. Experimental          field was silty clay loam in texture with available N (311.0 kg ha-1), P2O5 (15.7 kg ha-1), K2O (185 kg   ha-1).Statistical Design: Randomized Block Design (RBD).Methodology: The study consist of 8 treatment and replicated three times T1- 100% RDF + FYM 12.5 t ha-1 + 2% DAP, T2- 100% RDF + FYM 12.5t ha-1 + 2% Urea, T3- 100% RDF + FYM 12.5 t ha-1 + 40 ppm NAA spray, T4- 100% RDF + FYM 12.5 t ha-1 + 10 ppm Salicylic, T5-100% RDF + FYM 12.5 t ha-1 + 75 ppm GA3, T6- 100% RDF + FYM 12.5 t ha-1 + 5 kg TNAU Pulse wonder T7- 100% RDF + FYM 12.5 t ha-1 T8- Control.Results: Application of 100% RDF along with 12.5 t ha-1 FYM + 5 kg ha-1 TNAU pulse wonder resulted in Maximum nutrient NPK uptake, nutrient availability and agronomic efficiency was observed.Conclusion: Application of full dose of RDF along with FYM and TNAU pulse wonder reacted better in terms of NPK uptake, nutrient availability and agronomic efficiency when compared to other foliar treatment in the evaluation of nutrient balance and nutrient use efficiency.
研究地点和时间:2023-24 年 Rabi 季节,在哥印拜陀 Karunya 科技学院的教学农场进行了田间试验。试验田为淤泥质粘壤土,氮(311.0 千克/公顷-1)、磷(15.7 千克/公顷-1)、钾(185 千克/公顷-1):统计设计:随机区组设计(RBD):该研究共有 8 个处理,重复 3 次。T1- 100% RDF + FYM 12.5 t ha-1 + 2% DAP,T2- 100% RDF + FYM 12.5 t ha-1 + 2% Urea,T3- 100% RDF + FYM 12.5 t ha-1 + 40 ppm NAA spray,T4- 100% RDF + FYM 12.5 t ha-1 + 10 ppm NAA spray。5 t ha-1 + 10 ppm 水杨酸,T5-100% RDF + FYM 12.5 t ha-1 + 75 ppm GA3,T6- 100% RDF + FYM 12.5 t ha-1 + 5 kg TNAU Pulse wonder T7- 100% RDF + FYM 12.5 t ha-1 T8- Control.结果:结果:100% RDF + 12.5 t ha-1 FYM + 5 kg ha-1 TNAU Pulse wonder 的施用使 NPK 吸收率、养分利用率和农艺效率达到最高:结论:在养分平衡和养分利用效率评估中,与其他叶面处理相比,施用全剂量 RDF 以及 FYM 和 TNAU pulse wonder 在氮磷钾吸收、养分可用性和农艺效率方面反应更好。
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引用次数: 0
Evaluation of the Effects of Edible Coatings of Ocimum sanctum and Aloe vera on Jaggery Shelf Life 评估圣女果和芦荟食用涂层对琼浆保质期的影响
Pub Date : 2024-05-06 DOI: 10.9734/ejnfs/2024/v16i51428
Huma Rana, A. P. Garg, Sourabh Jain
Background: In western Uttar Pradesh, jaggery is a widespread cottage enterprise based on agriculture, and farmers are forced to sell their product at a lesser price when it is still fresh. Therefore, it was thought to be desirable to create better storage techniques in order to extend its shelf life.  Aloe vera, and Ocimum sanctum, often known as "Tulsi," are frequently utilised as antimicrobial food additives because they offer a host of other health advantages in addition to their well-known antibacterial qualities. Because the edible coatings made of these herbs provide a semi-permeable barrier to gases and water vapours, they may prolong the shelf life of jaggery by preventing degradation. Objective: The goal of the current study was to assess the ability of edible coatings of common Indian herbs, such as tulsi and Aloe vera,, to extend the shelf life of jaggery while maintaining attributes that are equal to those of fresh jaggery, in accordance with the guidelines set forth by the Food Safety and Standards Authority of India (FSSAI). Methodology: The physicochemical characteristics, antioxidant activity, total viable count, and antibacterial activity of tulsi-Aloe vera, coated (TAC), Ocimum sanctum, and Aloe vera, (AC) jaggery were assessed and compared with non-coated control. The physicochemical properties were ascertained using standard methodology for measurement of reducing sugars, proteins, phenols, saponins, tannin, alkaloids, and flavonoids. The antimicrobial activity was ascertained by means of the agar double diffusion method. According to established protocol, antioxidant activity was assessed using the DPPH radical scavenging assay and the reducing power assay. Results: According to the research, there is no discernible microbiological deterioration and the edible coatings containing tulsi and Aloe vera, extend the shelf life of jaggery during storage. When compared to uncoated jaggery, coatings were efficient at preventing the growth of both Gramme positive and Gramme negative microorganisms. Over the course of six months, the herb-infused coatings also retained their phenolic, flavonoid, and tannin contents, which improved their anti-oxidant efficacy when compared to the untreated control group.
背景:在北方邦西部,琼脂是一种普遍存在的以农业为基础的家庭作坊式企业,农民不得不在产品新鲜时以较低的价格出售。因此,人们认为应该创造更好的储存技术,以延长其保质期。 芦荟和通常被称为 "Tulsi "的圣女果经常被用作抗菌食品添加剂,因为它们除了众所周知的抗菌特性外,还具有许多其他健康优势。由于这些草药制成的可食用涂层对气体和水蒸气具有半渗透性的阻隔作用,因此可以通过防止降解来延长琼脂的保质期。研究目的本研究的目的是根据印度食品安全与标准局(FSSAI)制定的指导方针,评估土尔其和芦荟等常见印度草本植物的可食用涂层延长琼脂保质期的能力,同时保持与新鲜琼脂相同的属性。研究方法:评估了涂抹塔西-芦荟(TAC)、圣女果和芦荟(AC)琼脂的理化特性、抗氧化活性、总存活数和抗菌活性,并与非涂抹对照进行了比较。理化特性采用标准方法测定还原糖、蛋白质、酚类、皂甙、单宁、生物碱和黄酮类化合物。抗菌活性采用琼脂双扩散法进行测定。根据既定方案,采用 DPPH 自由基清除测定法和还原力测定法评估抗氧化活性。结果:研究结果表明,琼脂没有出现明显的微生物变质现象,含有土尔其和芦荟的可食用涂层延长了琼脂在储存期间的保质期。与无涂层的琼脂相比,涂层能有效防止革兰氏阳性和革兰氏阴性微生物的生长。在 6 个月的时间里,添加了草药的涂层还保留了其酚类、类黄酮和单宁含量,与未经处理的对照组相比,提高了其抗氧化功效。
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引用次数: 0
Antibiogram and Molecular Detection of Aflatoxigenic Gene from some Species of Aspergillus in Cereal Grains 谷物中一些曲霉菌种的黄曲霉致病基因的抗生素图谱和分子检测
Pub Date : 2024-05-01 DOI: 10.9734/ejnfs/2024/v16i51425
S. C. Ihechu, Nedie Patience Akani, T. Sampson
Aflatoxins are potent mycotoxins produced by certain strains of Aspergillus, which pose significant threats to human and animal health due to their carcinogenic and mutagenic properties. The study therefore targeted the antibiogram and molecular detection of aflatoxigenic gene from some species of Aspergillus in Cereal grains. Seventy-two samples of maize, rice, wheat and millet were bought from different vendors in Mile 3, Mile 1 and Rumuokoro Market in Port Harcourt, Rivers State, Nigeria. The molecular characterization and detection of the aflatoxin regulatory gene was achieved using a PCR-based technique. The disc diffusion method was used in determining the antibiogram of the isolates. Data showed that the species were closely related to Aspergillus flavus strain HBF576, Aspergillus flavus strain AS25, Aspergillus niger strain A40, Aspergillus flavus strain 64-A1, Aspergillus flavus, A1S6_8 and Aspergillus flavus strain AKF-10. More so, thirteen (86.7%) of the fungal isolates had the aflatoxin regulatory gene. The antibiogram showed that 100% of A. flavus and A. niger were completely susceptible to nystatin while 95.45% of A. flavus and 100% of A. niger were susceptible to itraconazole. Fluconazole was however, the least potent antifungal agent. The presence of aflatoxin-producing Aspergillus bearing the aflatoxin regulatory gene could be a concern to the public, especially with the documented effect of aflatoxin on human health. The study indicated a high in vitro growth inhibition activity of nystatin, which could therefore serve as a potent antifungal agent in the control and management of infections associated with these fungal isolates in cereal grains.
黄曲霉毒素是由某些曲霉菌株产生的强效霉菌毒素,由于其致癌和致突变的特性,对人类和动物的健康构成严重威胁。因此,这项研究以谷物中某些曲霉菌种的黄曲霉毒素基因的抗生素图谱和分子检测为目标。研究人员从尼日利亚河流州哈科特港 Mile 3、Mile 1 和 Rumuokoro 市场的不同商贩处购买了 72 份玉米、大米、小麦和小米样品。采用聚合酶链式反应(PCR)技术对黄曲霉毒素调控基因进行了分子鉴定和检测。在确定分离物的抗生素图谱时,采用了盘扩散法。数据显示,这些物种与黄曲霉菌株 HBF576、黄曲霉菌株 AS25、黑曲霉菌株 A40、黄曲霉菌株 64-A1、黄曲霉菌株 A1S6_8 和黄曲霉菌株 AKF-10 关系密切。此外,13 株(86.7%)真菌分离物含有黄曲霉毒素调控基因。抗生素图显示,100%的黄曲霉菌和黑曲霉菌对硝司他丁完全敏感,95.45%的黄曲霉菌和 100%的黑曲霉菌对伊曲康唑敏感。不过,氟康唑的抗真菌效果最差。带有黄曲霉毒素调控基因的黄曲霉能产生黄曲霉毒素,这可能会引起公众的关注,尤其是黄曲霉毒素对人类健康的影响已被证实。研究表明,硝司他丁具有很高的体外生长抑制活性,因此可作为一种有效的抗真菌剂,用于控制和处理谷物中与这些真菌分离物有关的感染。
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引用次数: 0
Amino Acid Compositions, In Vitro Antioxidant and Anti-diabetic Properties of Cookies from Wheat and Kidney Beans (Phaseolus vulgaris L.) Flour blends 小麦和芸豆(Phaseolus vulgaris L.)混合面粉饼干的氨基酸组成、体外抗氧化性和抗糖尿病特性
Pub Date : 2024-04-23 DOI: 10.9734/ejnfs/2024/v16i51420
Dele Ayorinde Olowookere, S. Malomo
This study evaluated the in vitro antioxidant and antidiabetic activities of the cookies produced from wheat and kidney bean composite flours at different ratios viz: 100:0 (WKB 1), 80:20 (WKB 2), 60:40 (WKB 3), 40:60 (WKB 4), respectively. The proximate compositions of the composite flour blends improved during the baking process into cookie, most especially the crude fibre (12.09-13.73%) and crude protein (18-21%) contents, respectively. The amino acid profiles of the cookies were well established with high biological values (>70%) with good essential, non-essential and hydrophobic amino acids while glutamic acid was mostly abundant in the cookies. The in-vitro antioxidant properties of the cookie samples were more potent (~80%) when compared with a standard ascorbic acid, a well-known antioxidant. Besides, the in-vitro anti-diabetic properties of the cookie samples were revealed through their improved α-amylase and α-glucosidase inhibition potentials (~70%) when compared with a standard acarbose, a well-known anti-diabetic drug. The weights (5.20-6.41 g), width (45.70-45.88 mm), thickness (5.03-5.06 mm) and spread ratio (11.89-12.26) of the cookies from the composite flours were significantly (P<0.05) comparable to the control (WKB 1) sample, respectively. This, however did not alter the organoleptic attributes of the composite cookie samples when compared to the commercial ones. We therefore concluded that the cookies rich in antioxidants and anti-diabetic potentials could be produced from wheat and kidney bean flour blends.
本研究评估了用小麦和芸豆复合面粉按不同比例(即 100:0 (WKB 1)、80:20 (WKB 2)、60:40 (WKB 3)、40:60 (WKB 4))制成的饼干的体外抗氧化和抗糖尿病活性:分别为 100:0 (WKB 1)、80:20 (WKB 2)、60:40 (WKB 3)、40:60 (WKB 4)。在饼干的烘焙过程中,复合面粉混合物的近似成分有所改善,尤其是粗纤维(12.09%-13.73%)和粗蛋白(18%-21%)含量。曲奇饼干的氨基酸谱很好,具有很高的生物价值(大于 70%),其中必需氨基酸、非必需氨基酸和疏水性氨基酸都很好,而谷氨酸在曲奇饼干中含量最高。与标准抗坏血酸(一种著名的抗氧化剂)相比,饼干样品的体外抗氧化性能更强(约为 80%)。此外,与标准的阿卡波糖(一种著名的抗糖尿病药物)相比,曲奇饼干样品的α-淀粉酶和α-葡萄糖苷酶抑制潜能得到了改善(约 70%),从而显示了曲奇饼干样品的体外抗糖尿病特性。复合面粉制成的饼干的重量(5.20-6.41 克)、宽度(45.70-45.88 毫米)、厚度(5.03-5.06 毫米)和铺展率(11.89-12.26)分别与对照组(WKB 1)样品有显著的可比性(P<0.05)。然而,与商用饼干相比,复合饼干样品的感官属性并没有改变。因此,我们得出结论:小麦和芸豆混合面粉可制成富含抗氧化剂和抗糖尿病潜力的饼干。
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European Journal of Nutrition &amp; Food Safety
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