Adelia Ratnadita, Christnawati, Pinandi Sri Pudyani
Introduction and Aim: Exposure to blue light-emitting diode (blue-LED) during the stabilization period can prevent relapse by increasing the tension side osteoblast, which is characterized by increased levels of alkaline phosphatase (ALP) in the gingival crevicular fluid (GCF). Increasing ALP levels occurred from day 7 with the highest peak on day 14 post-stabilization. The purpose of this research was to analyze the effect of blue-LED exposure during the stabilization period on ALP levels in the tension side GCF of Wistar rats (Rattus norvegicus) at days 0, 3, 7, and 14 post-stabilizations. Materials and Methods: Ten male Wistar rats aged 2.5-3 months, weighing 200-250 grams were divided into two groups (control and LED group). An orthodontic force of 35 grams was applied to the mandibular inter incisors of Wistar rats using an open coil spring. Blue-LED (490 nm wavelength, 1000 mW/cm² light intensity) exposure for 30 seconds once a day during a 7-day stabilization period. Gingival crevicular fluid was taken with paper points on days 0, 3, 7, and 14 post-stabilizations for ALP levels measurement using an ELISA method. Two-way ANOVA and Post Hoc LSD statistical tests were performed. Results: The results showed LED group ALP levels were higher than the control group. There has been a significant increase in ALP levels on days 7 and 14 in the control and LED groups. Conclusion: It was concluded that blue-LED exposure increased tension side ALP levels. Increasing ALP levels occurred from day 7 with the highest peak level on day 14. Blue-LED exposure in the stabilization period is a favorable therapeutic option to accelerate alveolar bone formation due to being non-invasive, easy to apply clinically, and low cost.
{"title":"Blue-light emitting diode exposure effect on alkaline phosphatase levels post-orthodontic stabilization in rats","authors":"Adelia Ratnadita, Christnawati, Pinandi Sri Pudyani","doi":"10.51248/.v43i3.2080","DOIUrl":"https://doi.org/10.51248/.v43i3.2080","url":null,"abstract":"Introduction and Aim: Exposure to blue light-emitting diode (blue-LED) during the stabilization period can prevent relapse by increasing the tension side osteoblast, which is characterized by increased levels of alkaline phosphatase (ALP) in the gingival crevicular fluid (GCF). Increasing ALP levels occurred from day 7 with the highest peak on day 14 post-stabilization. The purpose of this research was to analyze the effect of blue-LED exposure during the stabilization period on ALP levels in the tension side GCF of Wistar rats (Rattus norvegicus) at days 0, 3, 7, and 14 post-stabilizations.\u0000 \u0000Materials and Methods: Ten male Wistar rats aged 2.5-3 months, weighing 200-250 grams were divided into two groups (control and LED group). An orthodontic force of 35 grams was applied to the mandibular inter incisors of Wistar rats using an open coil spring. Blue-LED (490 nm wavelength, 1000 mW/cm² light intensity) exposure for 30 seconds once a day during a 7-day stabilization period. Gingival crevicular fluid was taken with paper points on days 0, 3, 7, and 14 post-stabilizations for ALP levels measurement using an ELISA method. Two-way ANOVA and Post Hoc LSD statistical tests were performed.\u0000 \u0000Results: The results showed LED group ALP levels were higher than the control group. There has been a significant increase in ALP levels on days 7 and 14 in the control and LED groups.\u0000 \u0000Conclusion: It was concluded that blue-LED exposure increased tension side ALP levels. Increasing ALP levels occurred from day 7 with the highest peak level on day 14. Blue-LED exposure in the stabilization period is a favorable therapeutic option to accelerate alveolar bone formation due to being non-invasive, easy to apply clinically, and low cost.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42319194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rishika Dhimole, Mithilesh M. Dhamande, Seema R. Kambala, Prinyanka Paul Madhu
In dentistry precisely recording the soft tissue details and adjacent tooth morphology is a critical process. It is quite challenging for the dentist since the oral hard and soft tissue structures show great variations in every patient. Conventional impression materials such as agar, alginate and elastomeric impression materials are used but these impression materials require patient’s cooperation during impression taking. These impression materials sometimes cause gagging, irritation and unpleasant taste and smell to the patient. To overcome these drawbacks digital impression and digital scanners are encouraging innovations. They increase the patient’s comfort and aids in the accuracy in recording tissue details. The development of new technology in the medical and dental fields is leading to advancements that enable physicians to create treatments and materials that can enhance patients' quality of life. Different IOSs have adopted varied scanning methodologies, which could result in different scanning accuracies. This was done to compare the accuracy of several IOSs and the effects of different variables on the accuracy result. Using 3D scanning technologies, a physical model which is digital three-dimensional computer-aided design (CAD) is transformed. For the design and production of specific parts using additive manufacturing (AM) technologies, this digital output offers several advantages. It is crucial to evaluate the dentistry industry's present level of 3D scanning technology utilization.
{"title":"The digital era in dentistry: A review","authors":"Rishika Dhimole, Mithilesh M. Dhamande, Seema R. Kambala, Prinyanka Paul Madhu","doi":"10.51248/.v43i3.2865","DOIUrl":"https://doi.org/10.51248/.v43i3.2865","url":null,"abstract":"In dentistry precisely recording the soft tissue details and adjacent tooth morphology is a critical process. It is quite challenging for the dentist since the oral hard and soft tissue structures show great variations in every patient. Conventional impression materials such as agar, alginate and elastomeric impression materials are used but these impression materials require patient’s cooperation during impression taking. These impression materials sometimes cause gagging, irritation and unpleasant taste and smell to the patient. To overcome these drawbacks digital impression and digital scanners are encouraging innovations. They increase the patient’s comfort and aids in the accuracy in recording tissue details. The development of new technology in the medical and dental fields is leading to advancements that enable physicians to create treatments and materials that can enhance patients' quality of life. Different IOSs have adopted varied scanning methodologies, which could result in different scanning accuracies. This was done to compare the accuracy of several IOSs and the effects of different variables on the accuracy result. Using 3D scanning technologies, a physical model which is digital three-dimensional computer-aided design (CAD) is transformed. For the design and production of specific parts using additive manufacturing (AM) technologies, this digital output offers several advantages. It is crucial to evaluate the dentistry industry's present level of 3D scanning technology utilization.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48209505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sumana Maiti Das, A. Bose, Suparna Kanti Pal, Suman Meyur, Siddhartha Das, Aloke Ghosh Dastidar
Introduction and Aim: Radiation induced skin reactions (RISR) often delays the treatment affecting compliance and outcomes. Granulocyte colony stimulating factor (G-CSF) is known to recruit dermo-genic stem cells and help in healing. Very few studies are there to evaluate its potential in this regard. This study evaluates intradermal infiltration of G-CSF for its effectiveness, effect on treatment delay and quality of life in comparison to conventional treatment for grade 3/4 RISR. Materials and Methods: Patients experiencing advanced RISR due to any regimen of external beam radiation above 50 Gy of 2 Gy equivalent doses were randomized to undergo treatment either with intradermal G-CSF infiltration at margin and bases of lesion along with conventional therapies or with only conventional anti-inflammatory/ antibiotic therapy. The experimental arm and the control arm had 62 and 60 patients respectively analyzed for time taken for re-epithelization and treatment delay. Also.pre and two weeks post-treatment global quality of life and pain scores were taken for qualitative comparison between the arms. Results: G-CSF arm took a mean 5.2 (95percent CI=4.4-5.7) days, significantly less than conventional treatment arm (Mean=7.5 days, 95percent CI= 6.8-8.1,p=0.002) for re-epithelization. Grade of toxicity adjusted hazard ratio of non-resolution in 10 days time was 2.98 for the control arm over the experimental arm(p=.046). Treatment gap was significantly less in the G-CSF arm (48.4 percent vs 68.3percent, p=0.026), with average delay of treatment being significantly reduced (p 0.001). A ten-day delay adjusted survival was significantly better in the experimental arm (Mantel-Cox Log rank test, X2=12.26, p 0.001). Conclusion: Intradermal perilesional G-CSF infiltration for advanced RISR hastens wound healing compared to conventional treatment and significantly reduces the chances of treatment delay.
{"title":"A randomised phase II trial of intra-dermal G-CSF infiltration in radiation induced skin reactions","authors":"Sumana Maiti Das, A. Bose, Suparna Kanti Pal, Suman Meyur, Siddhartha Das, Aloke Ghosh Dastidar","doi":"10.51248/.v43i3.736","DOIUrl":"https://doi.org/10.51248/.v43i3.736","url":null,"abstract":"Introduction and Aim: Radiation induced skin reactions (RISR) often delays the treatment affecting compliance and outcomes. Granulocyte colony stimulating factor (G-CSF) is known to recruit dermo-genic stem cells and help in healing. Very few studies are there to evaluate its potential in this regard. This study evaluates intradermal infiltration of G-CSF for its effectiveness, effect on treatment delay and quality of life in comparison to conventional treatment for grade 3/4 RISR.\u0000 \u0000Materials and Methods: Patients experiencing advanced RISR due to any regimen of external beam radiation above 50 Gy of 2 Gy equivalent doses were randomized to undergo treatment either with intradermal G-CSF infiltration at margin and bases of lesion along with conventional therapies or with only conventional anti-inflammatory/ antibiotic therapy. The experimental arm and the control arm had 62 and 60 patients respectively analyzed for time taken for re-epithelization and treatment delay. Also.pre and two weeks post-treatment global quality of life and pain scores were taken for qualitative comparison between the arms.\u0000 \u0000Results: G-CSF arm took a mean 5.2 (95percent CI=4.4-5.7) days, significantly less than conventional treatment arm (Mean=7.5 days, 95percent CI= 6.8-8.1,p=0.002) for re-epithelization. Grade of toxicity adjusted hazard ratio of non-resolution in 10 days time was 2.98 for the control arm over the experimental arm(p=.046). Treatment gap was significantly less in the G-CSF arm (48.4 percent vs 68.3percent, p=0.026), with average delay of treatment being significantly reduced (p 0.001). A ten-day delay adjusted survival was significantly better in the experimental arm (Mantel-Cox Log rank test, X2=12.26, p 0.001).\u0000 \u0000Conclusion: Intradermal perilesional G-CSF infiltration for advanced RISR hastens wound healing compared to conventional treatment and significantly reduces the chances of treatment delay.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45123700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Purushothaman, A. A. Al bshabshe, Alwadai Nasser Mohammed M., Naif Sulaiman, Omprakash Palanivel
Death is an assured inevitability event for all living beings of this cosmos. Based on the American Academy of Neurology (AAN) recommendations, clinical diagnosis of Brain Death (BD) includes multiple sequences of tests. The Apnea Test (AT) is a more significant procedure in diagnosing BD. Unfortunately, complications during this procedure can occur more frequently than documented, and its incidence is limited to case reports. However, most critical care physicians and Cardio-respiratory therapists consider an AT a principal method to confirm BD as it provides decisive information about the definitive loss of brainstem function. Here, we describe the execution of the AT procedure that transpired into extensive subcutaneous emphysema, tension pneumothorax, and pneumoperitoneum followed by pulseless electrical activity due to direct airway perforation by the supplemental oxygen catheter. In addition, we do a literature review on airway complications incidence and suggest methods to avoid such fatal complications. Finally, further prospective studies are recommended to evaluate the barotrauma incidence and complication nature during AT.
{"title":"Apnea test induced barotrauma in an obese subject with a short neck - A case report","authors":"S. Purushothaman, A. A. Al bshabshe, Alwadai Nasser Mohammed M., Naif Sulaiman, Omprakash Palanivel","doi":"10.51248/.v43i3.2224","DOIUrl":"https://doi.org/10.51248/.v43i3.2224","url":null,"abstract":"Death is an assured inevitability event for all living beings of this cosmos. Based on the American Academy of Neurology (AAN) recommendations, clinical diagnosis of Brain Death (BD) includes multiple sequences of tests. The Apnea Test (AT) is a more significant procedure in diagnosing BD. Unfortunately, complications during this procedure can occur more frequently than documented, and its incidence is limited to case reports. However, most critical care physicians and Cardio-respiratory therapists consider an AT a principal method to confirm BD as it provides decisive information about the definitive loss of brainstem function. Here, we describe the execution of the AT procedure that transpired into extensive subcutaneous emphysema, tension pneumothorax, and pneumoperitoneum followed by pulseless electrical activity due to direct airway perforation by the supplemental oxygen catheter. In addition, we do a literature review on airway complications incidence and suggest methods to avoid such fatal complications. Finally, further prospective studies are recommended to evaluate the barotrauma incidence and complication nature during AT.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45506055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Diabetes mellitus (DM) will increasingly contribute to years of life and disability worldwide. Approximately 25% of individuals with diabetes will develop diabetic foot ulcers. This study aimed to assess the effectiveness of microcurrent therapy compared to low-level laser therapy in terms of wound healing and quality of life among patients with diabetic foot ulcers. Materials and Methods: This study followed a prospective randomized open blinded endpoint assessment (PROBE) design with two parallel groups allocated in a 1:1 ratio. Participants meeting the selection criteria and having diabetic foot ulcers were included. Baseline measurements were taken before randomly assigning participants to the treatment groups. Pre-test and post-test data were collected to evaluate the wound size. Results: The t-test analyses demonstrated significant improvement in both treatment groups (LLLT: t = 41.818, p < 0.001; Micro-Current Group: t = 32.787, p < 0.001). To compare the groups, One-Way Analysis of Variance (ANOVA) was conducted, indicating a significant difference in ulcer size between the LLLT group (mean value: 62.600, SD=3.035) and the microcurrent therapy group (mean value: 51.933, SD= 6.938). Conclusion: This study concludes that microcurrent therapy is more effective than low-level laser therapy for managing diabetic foot ulcers. The findings suggest that a 3-week intensive physiotherapy therapeutic approach, combined with conventional medical treatment, yields the best results in terms of wound healing.
{"title":"Efficacy of microcurrent therapy versus laser therapy for diabetic foot ulcer on size of the wound","authors":"K. M., C. S, Shruthi Kamal V.","doi":"10.51248/.v43i3.2696","DOIUrl":"https://doi.org/10.51248/.v43i3.2696","url":null,"abstract":"Introduction and Aim: Diabetes mellitus (DM) will increasingly contribute to years of life and disability worldwide. Approximately 25% of individuals with diabetes will develop diabetic foot ulcers. This study aimed to assess the effectiveness of microcurrent therapy compared to low-level laser therapy in terms of wound healing and quality of life among patients with diabetic foot ulcers.\u0000 \u0000Materials and Methods: This study followed a prospective randomized open blinded endpoint assessment (PROBE) design with two parallel groups allocated in a 1:1 ratio. Participants meeting the selection criteria and having diabetic foot ulcers were included. Baseline measurements were taken before randomly assigning participants to the treatment groups. Pre-test and post-test data were collected to evaluate the wound size.\u0000 \u0000Results: The t-test analyses demonstrated significant improvement in both treatment groups (LLLT: t = 41.818, p < 0.001; Micro-Current Group: t = 32.787, p < 0.001). To compare the groups, One-Way Analysis of Variance (ANOVA) was conducted, indicating a significant difference in ulcer size between the LLLT group (mean value: 62.600, SD=3.035) and the microcurrent therapy group (mean value: 51.933, SD= 6.938).\u0000 \u0000Conclusion: This study concludes that microcurrent therapy is more effective than low-level laser therapy for managing diabetic foot ulcers. The findings suggest that a 3-week intensive physiotherapy therapeutic approach, combined with conventional medical treatment, yields the best results in terms of wound healing.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49347505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Temporomandibular joint internal derangement (TMJ ID) may be managed by conservative means, minimally invasive methods, and/ or surgically. Conservative options include soft diet, pharmacotherapy, splints, physiotherapy, or topical administration of nutraceuticals, for elimination of TMJ ID symptoms. FIXIT is a topical ointment, consisting of plant-based ingredients; its specific components have been successful in treating knee osteoarthritis (OA). However, these have not been applied for TMJ ID conditions (lacuna in literature). The aim of this study is, therefore, to evaluate the effectiveness of FIXIT in TMJ ID management by assessing TMJ pain, clicking sound and pain-free maximum mouth opening (MMO) in an 18-year-old male. The patient complained of right TMJ pain (VAS- 8) on mouth opening, and loud clicking sounds, with pain-free MMO of 19 mm pre-treatment. Post-topical therapy (after 3 weeks) with FIXIT, significant reduction in TMJ pain (VAS- 0) and joint sounds (absent- 0), with improved MMO (47 mm) was noted. No recurrence of symptoms was observed after 3 months of follow-up. FIXIT has been effective in alleviating TMJ ID symptoms, thereby enhancing overall jaw function. Its ability to eliminate clicking sounds was found to be an unprecedented finding; suggesting it’ novelty as a non-surgical intervention for TMJ ID.
{"title":"A novel conservative modality in the management of internal derangement of temporomandibular joint- A case report","authors":"C. Ravikumar, Krishnakumar Raja V.B., Sasikala B.","doi":"10.51248/.v43i3.2747","DOIUrl":"https://doi.org/10.51248/.v43i3.2747","url":null,"abstract":"Temporomandibular joint internal derangement (TMJ ID) may be managed by conservative means, minimally invasive methods, and/ or surgically. Conservative options include soft diet, pharmacotherapy, splints, physiotherapy, or topical administration of nutraceuticals, for elimination of TMJ ID symptoms. FIXIT is a topical ointment, consisting of plant-based ingredients; its specific components have been successful in treating knee osteoarthritis (OA). However, these have not been applied for TMJ ID conditions (lacuna in literature). The aim of this study is, therefore, to evaluate the effectiveness of FIXIT in TMJ ID management by assessing TMJ pain, clicking sound and pain-free maximum mouth opening (MMO) in an 18-year-old male. The patient complained of right TMJ pain (VAS- 8) on mouth opening, and loud clicking sounds, with pain-free MMO of 19 mm pre-treatment. Post-topical therapy (after 3 weeks) with FIXIT, significant reduction in TMJ pain (VAS- 0) and joint sounds (absent- 0), with improved MMO (47 mm) was noted. No recurrence of symptoms was observed after 3 months of follow-up. FIXIT has been effective in alleviating TMJ ID symptoms, thereby enhancing overall jaw function. Its ability to eliminate clicking sounds was found to be an unprecedented finding; suggesting it’ novelty as a non-surgical intervention for TMJ ID.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42547750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abhijnya K Renukaprasad, E. Suman, Udayalaxmi Jeppu, S. Paul
Introduction and Aim: Hospital-acquired infection with multidrug-resistant bacteria following urinary catheter use in hospitalized patients is mainly due to the biofilm-forming ability of these bacteria. It becomes essential to look at the other options of treatment of such urinary tract infections (UTI) and one such option has been the use of bacteriophages. The study aimed to compare the biofilms formed by uropathogenic Escherichia coli on three different types of urinary catheters in the presence of crude coliphage and sub-inhibitory concentration of ciprofloxacin. Materials and Methods: It was an Institution based experimental study. A total of 10 red rubber (latex) catheter pieces, 10 Foley (PVC) catheter pieces and 10 silicone Foley catheter pieces were used for the study. Crude coliphage suspension was prepared from sewage samples taken from the sewage treatment plant. Biofilm production by ten uropathogenic E. coli isolates on these catheters was determined by Kolter and O’Toole’s method with certain modifications. Biofilm assay was repeated with conditions using crude bacteriophage and sub-inhibitory concentration. An electron micrograph of the catheter pieces was taken to visualize the biofilm formed. P value of 0.05 was considered statistically significant. Results: It was observed that, in the presence of crude bacteriophage there was a significant reduction in the biofilm formation on all three catheters (p 0.05). Also, there was a significant reduction in biofilm production in the presence of sub-inhibitory concentration of ciprofloxacin on silicone Foley’s catheter (p=0.001) but not on red rubber catheter or PVC Foley’s catheter (p 0.05). It was also observed that in some of the isolates the colony counts increased following the addition of ciprofloxacin in case of PVC Foley catheters. Conclusion: Bacteriophage-coated catheters may be the gateway to overcoming the hurdle of multidrug-resistant catheter-associated urinary tract infection.
{"title":"Promising catheters to reduce hospital-acquired urinary tract infections","authors":"Abhijnya K Renukaprasad, E. Suman, Udayalaxmi Jeppu, S. Paul","doi":"10.51248/.v43i3.2603","DOIUrl":"https://doi.org/10.51248/.v43i3.2603","url":null,"abstract":"Introduction and Aim: Hospital-acquired infection with multidrug-resistant bacteria following urinary catheter use in hospitalized patients is mainly due to the biofilm-forming ability of these bacteria. It becomes essential to look at the other options of treatment of such urinary tract infections (UTI) and one such option has been the use of bacteriophages. The study aimed to compare the biofilms formed by uropathogenic Escherichia coli on three different types of urinary catheters in the presence of crude coliphage and sub-inhibitory concentration of ciprofloxacin.\u0000 \u0000Materials and Methods: It was an Institution based experimental study. A total of 10 red rubber (latex) catheter pieces, 10 Foley (PVC) catheter pieces and 10 silicone Foley catheter pieces were used for the study. Crude coliphage suspension was prepared from sewage samples taken from the sewage treatment plant. Biofilm production by ten uropathogenic E. coli isolates on these catheters was determined by Kolter and O’Toole’s method with certain modifications. Biofilm assay was repeated with conditions using crude bacteriophage and sub-inhibitory concentration. An electron micrograph of the catheter pieces was taken to visualize the biofilm formed. P value of 0.05 was considered statistically significant.\u0000 \u0000Results: It was observed that, in the presence of crude bacteriophage there was a significant reduction in the biofilm formation on all three catheters (p 0.05). Also, there was a significant reduction in biofilm production in the presence of sub-inhibitory concentration of ciprofloxacin on silicone Foley’s catheter (p=0.001) but not on red rubber catheter or PVC Foley’s catheter (p 0.05). It was also observed that in some of the isolates the colony counts increased following the addition of ciprofloxacin in case of PVC Foley catheters.\u0000 \u0000Conclusion: Bacteriophage-coated catheters may be the gateway to overcoming the hurdle of multidrug-resistant catheter-associated urinary tract infection.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41628557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Sahu, Ranjitha Rao, Chaithra G.V., Cheryl Sarah Philipose, Sneha Rao A. R., Vatsala K.B.
Amniotic band syndrome (ABS) is a rare condition in which there is entrapment of the growing foetus or its organs by the band of tissue formed from the amniotic membrane. We received 20 weeks’ gestation-old female foetus for pathological examination. On external gross examination foetus was detected with only the thumb in the right hand with the absence of the remaining four fingers. On thorough examination there was membranous structure attached to the right hand running towards the right leg and partly attached to the toes. This on microscopy showed fibrosis. ABS is a rare syndrome with varied presentations which sometimes can endanger the life of the foetus. Though exact aetiology is unknown till date various risk factors are attributed to the condition.
{"title":"Amniotic band syndrome- A rare case report","authors":"K. Sahu, Ranjitha Rao, Chaithra G.V., Cheryl Sarah Philipose, Sneha Rao A. R., Vatsala K.B.","doi":"10.51248/.v43i3.2495","DOIUrl":"https://doi.org/10.51248/.v43i3.2495","url":null,"abstract":"Amniotic band syndrome (ABS) is a rare condition in which there is entrapment of the growing foetus or its organs by the band of tissue formed from the amniotic membrane. We received 20 weeks’ gestation-old female foetus for pathological examination. On external gross examination foetus was detected with only the thumb in the right hand with the absence of the remaining four fingers. On thorough examination there was membranous structure attached to the right hand running towards the right leg and partly attached to the toes. This on microscopy showed fibrosis. ABS is a rare syndrome with varied presentations which sometimes can endanger the life of the foetus. Though exact aetiology is unknown till date various risk factors are attributed to the condition.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44544590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. F., Vadlapudi Kumar, V. E., Poornima D.V., Anuradha C.M., Prathap H.M.
Introduction and Aim: Gnidia glauca (Fresen.) Gilg (G. glauca) is an abundant source of phenolic and flavonoid compounds with therapeutic potential, despite this very few investigations conducted for enhanced phenolic and flavonoid production by suspension culture. In the present study a non-destructive harvesting method was developed for phenolics and flavonoids production in cell suspension of G. glauca using methyl jasmonate (MeJA) as a potent elicitor for product enhancement. Materials and Methods: cell suspension culture was initiated to evaluate the elicitor impact on cell growth, Phytochemical extraction and screening, total phenolic and flavonoid content, antioxidant assay by 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, was done to evaluate, its antioxidant capacity in the methyl jasmonate elicited suspension cultured sample of G. glauca. Results: Methyl jasmonate elicitation in response to 50 µM dosage showed an increase in biomass production of phenolic compounds and flavonoids after 6 days of culture incubation. Qualitative screening of phytoconstituents revealed the abundance of flavonoids and phenolics tannins, confirmed by UV-Visible spectrophotometry, TLC and HPLC analyses. G. glauca cell suspension culture sample showed dose dependent DPPH free radical scavenging activity compared to the respective control samples. Conclusion: The outcome of the present study shows that G.glauca MeJA (methyl jasmonate) elicitation at 50µM dosage elicited the highest presence of phenolics and flavonoid with potential antioxidant capability. Considering the vulnerability of G.glauca as enlisted in endangered plants list, the present protocol is a non-destructive harvesting method to produce biologically active phenolics and flavonoids of the plant.
介绍与目的:灰刺荆(freen .)Gilg (G. glauca)是具有治疗潜力的酚类和类黄酮化合物的丰富来源，尽管通过悬浮培养提高酚类和类黄酮产量的研究很少。本研究利用茉莉酸甲酯(MeJA)作为强效激发子，建立了一种非破坏性的收获方法，用于青花毛藻细胞悬浮液中酚类物质和黄酮类物质的生产。材料与方法:通过细胞悬浮培养，评价激发子对细胞生长、植物化学提取与筛选、总酚类和类黄酮含量的影响，采用抗氧化法测定1,1二苯基-2-苦味酰肼(DPPH)自由基清除能力，在茉莉酸甲酯诱导的glauca悬浮培养样品中评价其抗氧化能力。结果:茉莉酸甲酯对50µM剂量的诱导，在培养6天后，酚类化合物和黄酮类物质的生物量增加。通过紫外-可见分光光度法、薄层色谱法和高效液相色谱法对其进行定性筛选，发现黄酮类化合物和酚类化合物单宁含量丰富。青光眼细胞悬浮培养样品与对照样品相比，具有剂量依赖性的DPPH自由基清除活性。结论:本研究结果表明，50µM剂量的茉莉酸甲酯(MeJA)诱导产生的酚类物质和类黄酮含量最高，具有潜在的抗氧化能力。考虑到青茅属濒危植物的脆弱性，本方案采用非破坏性采收方法，从青茅中提取具有生物活性的酚类物质和类黄酮。
{"title":"A non-destructive harvesting method to produce biologically active phenolics and flavonoids of Gnidia glauca (Fresen.) Gilg","authors":"R. F., Vadlapudi Kumar, V. E., Poornima D.V., Anuradha C.M., Prathap H.M.","doi":"10.51248/.v43i3.3191","DOIUrl":"https://doi.org/10.51248/.v43i3.3191","url":null,"abstract":"Introduction and Aim: Gnidia glauca (Fresen.) Gilg (G. glauca) is an abundant source of phenolic and flavonoid compounds with therapeutic potential, despite this very few investigations conducted for enhanced phenolic and flavonoid production by suspension culture. In the present study a non-destructive harvesting method was developed for phenolics and flavonoids production in cell suspension of G. glauca using methyl jasmonate (MeJA) as a potent elicitor for product enhancement. \u0000 \u0000Materials and Methods: cell suspension culture was initiated to evaluate the elicitor impact on cell growth, Phytochemical extraction and screening, total phenolic and flavonoid content, antioxidant assay by 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, was done to evaluate, its antioxidant capacity in the methyl jasmonate elicited suspension cultured sample of G. glauca. \u0000 \u0000Results: Methyl jasmonate elicitation in response to 50 µM dosage showed an increase in biomass production of phenolic compounds and flavonoids after 6 days of culture incubation. Qualitative screening of phytoconstituents revealed the abundance of flavonoids and phenolics tannins, confirmed by UV-Visible spectrophotometry, TLC and HPLC analyses. G. glauca cell suspension culture sample showed dose dependent DPPH free radical scavenging activity compared to the respective control samples. \u0000 \u0000Conclusion: The outcome of the present study shows that G.glauca MeJA (methyl jasmonate) elicitation at 50µM dosage elicited the highest presence of phenolics and flavonoid with potential antioxidant capability. Considering the vulnerability of G.glauca as enlisted in endangered plants list, the present protocol is a non-destructive harvesting method to produce biologically active phenolics and flavonoids of the plant.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48593328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: This study was aimed to investigate the impact of TENS (Transcutaneous Electrical Nerve Stimulation) along with Mckenzie lumbar extension exercise on patients with lumbar radiculopathy. The objective is to compare the effect of TENS with Mckenzie exercise and control group. The study assessed pain levels, radicular pain through SLR (Straight Leg Raise) test, disability using RMDQ (Roland Morris Disability Questionnaire), and spine range of motion (ROM) using MMST (Modified Modified Schober Test). Pre-test and post-test measurements were collected and compared to determine any differences. Methodology: The study included a total of 50 male and female patients aged between 31 and 40 years, all of whom experienced disabling pain for a duration of 6-12 weeks. The materials used for the assessment included an inch tape for measuring lumbar spine ROM, a goniometer for SLR, and a belt. Results: After the 6-week program, the findings revealed a significant reduction in pain levels, improved SLR, enhanced lumbar spine ROM, and decreased disability among the participants. Paired t-test showed significant differences in post-test values and independent t-test revealed significant post-test values in the experimental group than the control group at p<0.05. Conclusion: The group of patients with lumbar radiculopathy who received TENS combined with Mckenzie exercise demonstrated superior outcomes in terms of pain reduction, SLR improvement, MODI (Modified Oswestry Disability Index) scores, and RMQ (Roland Morris Questionnaire) scores compared to the group treated with general exercise and TENS.
{"title":"Effect of Mckenzie lumbar extension exercise with TENS on lumbar radiculopathy","authors":"S. Kumar B., Divyasree S., K. A, Surya Vishnuram","doi":"10.51248/.v43i3.2013","DOIUrl":"https://doi.org/10.51248/.v43i3.2013","url":null,"abstract":"Introduction and Aim: This study was aimed to investigate the impact of TENS (Transcutaneous Electrical Nerve Stimulation) along with Mckenzie lumbar extension exercise on patients with lumbar radiculopathy. The objective is to compare the effect of TENS with Mckenzie exercise and control group. The study assessed pain levels, radicular pain through SLR (Straight Leg Raise) test, disability using RMDQ (Roland Morris Disability Questionnaire), and spine range of motion (ROM) using MMST (Modified Modified Schober Test). Pre-test and post-test measurements were collected and compared to determine any differences.\u0000 \u0000Methodology: The study included a total of 50 male and female patients aged between 31 and 40 years, all of whom experienced disabling pain for a duration of 6-12 weeks. The materials used for the assessment included an inch tape for measuring lumbar spine ROM, a goniometer for SLR, and a belt.\u0000 \u0000Results: After the 6-week program, the findings revealed a significant reduction in pain levels, improved SLR, enhanced lumbar spine ROM, and decreased disability among the participants. Paired t-test showed significant differences in post-test values and independent t-test revealed significant post-test values in the experimental group than the control group at p<0.05.\u0000 \u0000Conclusion: The group of patients with lumbar radiculopathy who received TENS combined with Mckenzie exercise demonstrated superior outcomes in terms of pain reduction, SLR improvement, MODI (Modified Oswestry Disability Index) scores, and RMQ (Roland Morris Questionnaire) scores compared to the group treated with general exercise and TENS.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43960908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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