K. Sahu, Ranjitha Rao, Chaithra G.V., Cheryl Sarah Philipose, Sneha Rao A. R., Vatsala K.B.
Amniotic band syndrome (ABS) is a rare condition in which there is entrapment of the growing foetus or its organs by the band of tissue formed from the amniotic membrane. We received 20 weeks’ gestation-old female foetus for pathological examination. On external gross examination foetus was detected with only the thumb in the right hand with the absence of the remaining four fingers. On thorough examination there was membranous structure attached to the right hand running towards the right leg and partly attached to the toes. This on microscopy showed fibrosis. ABS is a rare syndrome with varied presentations which sometimes can endanger the life of the foetus. Though exact aetiology is unknown till date various risk factors are attributed to the condition.
{"title":"Amniotic band syndrome- A rare case report","authors":"K. Sahu, Ranjitha Rao, Chaithra G.V., Cheryl Sarah Philipose, Sneha Rao A. R., Vatsala K.B.","doi":"10.51248/.v43i3.2495","DOIUrl":"https://doi.org/10.51248/.v43i3.2495","url":null,"abstract":"Amniotic band syndrome (ABS) is a rare condition in which there is entrapment of the growing foetus or its organs by the band of tissue formed from the amniotic membrane. We received 20 weeks’ gestation-old female foetus for pathological examination. On external gross examination foetus was detected with only the thumb in the right hand with the absence of the remaining four fingers. On thorough examination there was membranous structure attached to the right hand running towards the right leg and partly attached to the toes. This on microscopy showed fibrosis. ABS is a rare syndrome with varied presentations which sometimes can endanger the life of the foetus. Though exact aetiology is unknown till date various risk factors are attributed to the condition.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44544590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. F., Vadlapudi Kumar, V. E., Poornima D.V., Anuradha C.M., Prathap H.M.
Introduction and Aim: Gnidia glauca (Fresen.) Gilg (G. glauca) is an abundant source of phenolic and flavonoid compounds with therapeutic potential, despite this very few investigations conducted for enhanced phenolic and flavonoid production by suspension culture. In the present study a non-destructive harvesting method was developed for phenolics and flavonoids production in cell suspension of G. glauca using methyl jasmonate (MeJA) as a potent elicitor for product enhancement. Materials and Methods: cell suspension culture was initiated to evaluate the elicitor impact on cell growth, Phytochemical extraction and screening, total phenolic and flavonoid content, antioxidant assay by 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, was done to evaluate, its antioxidant capacity in the methyl jasmonate elicited suspension cultured sample of G. glauca. Results: Methyl jasmonate elicitation in response to 50 µM dosage showed an increase in biomass production of phenolic compounds and flavonoids after 6 days of culture incubation. Qualitative screening of phytoconstituents revealed the abundance of flavonoids and phenolics tannins, confirmed by UV-Visible spectrophotometry, TLC and HPLC analyses. G. glauca cell suspension culture sample showed dose dependent DPPH free radical scavenging activity compared to the respective control samples. Conclusion: The outcome of the present study shows that G.glauca MeJA (methyl jasmonate) elicitation at 50µM dosage elicited the highest presence of phenolics and flavonoid with potential antioxidant capability. Considering the vulnerability of G.glauca as enlisted in endangered plants list, the present protocol is a non-destructive harvesting method to produce biologically active phenolics and flavonoids of the plant.
介绍与目的:灰刺荆(freen .)Gilg (G. glauca)是具有治疗潜力的酚类和类黄酮化合物的丰富来源，尽管通过悬浮培养提高酚类和类黄酮产量的研究很少。本研究利用茉莉酸甲酯(MeJA)作为强效激发子，建立了一种非破坏性的收获方法，用于青花毛藻细胞悬浮液中酚类物质和黄酮类物质的生产。材料与方法:通过细胞悬浮培养，评价激发子对细胞生长、植物化学提取与筛选、总酚类和类黄酮含量的影响，采用抗氧化法测定1,1二苯基-2-苦味酰肼(DPPH)自由基清除能力，在茉莉酸甲酯诱导的glauca悬浮培养样品中评价其抗氧化能力。结果:茉莉酸甲酯对50µM剂量的诱导，在培养6天后，酚类化合物和黄酮类物质的生物量增加。通过紫外-可见分光光度法、薄层色谱法和高效液相色谱法对其进行定性筛选，发现黄酮类化合物和酚类化合物单宁含量丰富。青光眼细胞悬浮培养样品与对照样品相比，具有剂量依赖性的DPPH自由基清除活性。结论:本研究结果表明，50µM剂量的茉莉酸甲酯(MeJA)诱导产生的酚类物质和类黄酮含量最高，具有潜在的抗氧化能力。考虑到青茅属濒危植物的脆弱性，本方案采用非破坏性采收方法，从青茅中提取具有生物活性的酚类物质和类黄酮。
{"title":"A non-destructive harvesting method to produce biologically active phenolics and flavonoids of Gnidia glauca (Fresen.) Gilg","authors":"R. F., Vadlapudi Kumar, V. E., Poornima D.V., Anuradha C.M., Prathap H.M.","doi":"10.51248/.v43i3.3191","DOIUrl":"https://doi.org/10.51248/.v43i3.3191","url":null,"abstract":"Introduction and Aim: Gnidia glauca (Fresen.) Gilg (G. glauca) is an abundant source of phenolic and flavonoid compounds with therapeutic potential, despite this very few investigations conducted for enhanced phenolic and flavonoid production by suspension culture. In the present study a non-destructive harvesting method was developed for phenolics and flavonoids production in cell suspension of G. glauca using methyl jasmonate (MeJA) as a potent elicitor for product enhancement. \u0000 \u0000Materials and Methods: cell suspension culture was initiated to evaluate the elicitor impact on cell growth, Phytochemical extraction and screening, total phenolic and flavonoid content, antioxidant assay by 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, was done to evaluate, its antioxidant capacity in the methyl jasmonate elicited suspension cultured sample of G. glauca. \u0000 \u0000Results: Methyl jasmonate elicitation in response to 50 µM dosage showed an increase in biomass production of phenolic compounds and flavonoids after 6 days of culture incubation. Qualitative screening of phytoconstituents revealed the abundance of flavonoids and phenolics tannins, confirmed by UV-Visible spectrophotometry, TLC and HPLC analyses. G. glauca cell suspension culture sample showed dose dependent DPPH free radical scavenging activity compared to the respective control samples. \u0000 \u0000Conclusion: The outcome of the present study shows that G.glauca MeJA (methyl jasmonate) elicitation at 50µM dosage elicited the highest presence of phenolics and flavonoid with potential antioxidant capability. Considering the vulnerability of G.glauca as enlisted in endangered plants list, the present protocol is a non-destructive harvesting method to produce biologically active phenolics and flavonoids of the plant.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48593328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: This study was aimed to investigate the impact of TENS (Transcutaneous Electrical Nerve Stimulation) along with Mckenzie lumbar extension exercise on patients with lumbar radiculopathy. The objective is to compare the effect of TENS with Mckenzie exercise and control group. The study assessed pain levels, radicular pain through SLR (Straight Leg Raise) test, disability using RMDQ (Roland Morris Disability Questionnaire), and spine range of motion (ROM) using MMST (Modified Modified Schober Test). Pre-test and post-test measurements were collected and compared to determine any differences. Methodology: The study included a total of 50 male and female patients aged between 31 and 40 years, all of whom experienced disabling pain for a duration of 6-12 weeks. The materials used for the assessment included an inch tape for measuring lumbar spine ROM, a goniometer for SLR, and a belt. Results: After the 6-week program, the findings revealed a significant reduction in pain levels, improved SLR, enhanced lumbar spine ROM, and decreased disability among the participants. Paired t-test showed significant differences in post-test values and independent t-test revealed significant post-test values in the experimental group than the control group at p<0.05. Conclusion: The group of patients with lumbar radiculopathy who received TENS combined with Mckenzie exercise demonstrated superior outcomes in terms of pain reduction, SLR improvement, MODI (Modified Oswestry Disability Index) scores, and RMQ (Roland Morris Questionnaire) scores compared to the group treated with general exercise and TENS.
{"title":"Effect of Mckenzie lumbar extension exercise with TENS on lumbar radiculopathy","authors":"S. Kumar B., Divyasree S., K. A, Surya Vishnuram","doi":"10.51248/.v43i3.2013","DOIUrl":"https://doi.org/10.51248/.v43i3.2013","url":null,"abstract":"Introduction and Aim: This study was aimed to investigate the impact of TENS (Transcutaneous Electrical Nerve Stimulation) along with Mckenzie lumbar extension exercise on patients with lumbar radiculopathy. The objective is to compare the effect of TENS with Mckenzie exercise and control group. The study assessed pain levels, radicular pain through SLR (Straight Leg Raise) test, disability using RMDQ (Roland Morris Disability Questionnaire), and spine range of motion (ROM) using MMST (Modified Modified Schober Test). Pre-test and post-test measurements were collected and compared to determine any differences.\u0000 \u0000Methodology: The study included a total of 50 male and female patients aged between 31 and 40 years, all of whom experienced disabling pain for a duration of 6-12 weeks. The materials used for the assessment included an inch tape for measuring lumbar spine ROM, a goniometer for SLR, and a belt.\u0000 \u0000Results: After the 6-week program, the findings revealed a significant reduction in pain levels, improved SLR, enhanced lumbar spine ROM, and decreased disability among the participants. Paired t-test showed significant differences in post-test values and independent t-test revealed significant post-test values in the experimental group than the control group at p<0.05.\u0000 \u0000Conclusion: The group of patients with lumbar radiculopathy who received TENS combined with Mckenzie exercise demonstrated superior outcomes in terms of pain reduction, SLR improvement, MODI (Modified Oswestry Disability Index) scores, and RMQ (Roland Morris Questionnaire) scores compared to the group treated with general exercise and TENS.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43960908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mamatha Shetty, Deepa Sara John, S. Durgapal, U. Shetty
Herpes simplex virus (HSV) is a double-stranded DNA virus that exists in two forms- HSV 1 and HSV 2. Oral herpetic lesions are typically caused by HSV-1. In the oral cavity, they are known to cause painful lesions that most commonly involve gingiva, palate, and buccal mucosa. Since it is a contagious infection and has the potential to cause complications in the host, timely diagnosis and management are important. Therefore, dental professionals must be aware of clinical presentation and appropriate diagnostic and management measures. The following case report discusses a case of Herpes Simplex Virus in a 33-year-old, systemically healthy female patient, who reported to the dental OPD with oral symptoms. The clinical features, investigations, diagnosis, and management of the condition have been elaborated in the review.
{"title":"Herpes simplex virus: A case report and review of literature","authors":"Mamatha Shetty, Deepa Sara John, S. Durgapal, U. Shetty","doi":"10.51248/.v43i3.2099","DOIUrl":"https://doi.org/10.51248/.v43i3.2099","url":null,"abstract":"Herpes simplex virus (HSV) is a double-stranded DNA virus that exists in two forms- HSV 1 and HSV 2. Oral herpetic lesions are typically caused by HSV-1. In the oral cavity, they are known to cause painful lesions that most commonly involve gingiva, palate, and buccal mucosa. Since it is a contagious infection and has the potential to cause complications in the host, timely diagnosis and management are important. Therefore, dental professionals must be aware of clinical presentation and appropriate diagnostic and management measures. The following case report discusses a case of Herpes Simplex Virus in a 33-year-old, systemically healthy female patient, who reported to the dental OPD with oral symptoms. The clinical features, investigations, diagnosis, and management of the condition have been elaborated in the review.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44864466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Crude herbal drugs and commercial extracts obtained from plant sources continue to play a significant role in healthcare, particularly in developing countries where traditional medicine practices are deeply rooted. The aim of the study was to estimate the antioxidant (DPPH) and cytotoxic effects of the ethanolic extract of Flaveria trinervia on raw 246.7 cells. Materials and Methods: In the Indian town of Thoothukudi's Kovilpatti village, F. trinervia was isolated. The entire plant was air-dried before being crushed, and 1 kg of the powdered ethanolic extract was employed in the Soxhlet apparatus for continuous extraction. By utilizing GC-MS analysis, F. trinervia ethanolic extract phytochemical assessment was studied. The antioxidant potential of F. trinervia was examined using the DPPH test. Raw 246.7 cell lines were used to test the ethanolic extract of F. trinervia for cytotoxic activity. Results: F. trinervia extracts were found to contain a variety of biologically active phytoconstituents, including ergosterol, octadecanoic acid, propanoic acid, and isopropyl palmitate, according to phytochemical analysis. Compared to the negative control, the ethanolic extract of F. trinervia considerably showed antioxidant activity and cytotoxicity. Ascorbic acid antioxidant activity with DPPH was 98.12%, whereas F.trinervia ethanolic extract's antioxidant activity was 78.91%. The anticancer efficacy of F. trinervia ethanolic extract against uncultured 246.7 cells was evaluated using the MTT assay. By charting cell viability vs extract concentration, the IC50 value was determined. The MTT assay identified raw cells IC50 at 24 hrs as 200 µg/mL of ethanolic F. trinervia extract. Conclusion: Active phytoconstituents are present in the F. trinervia whole plant ethanolic extract. Because of the presence of phytoconstituents, the extracts have excellent antioxidant activity and good cytotoxicity activity against raw 246.7 cell lines. The study recommended using whole plant extract from F. trinervia to treat several metabolic diseases.
{"title":"Phytochemical analysis, in-vitro antioxidant and cytotoxicity activities of Flaveria trinervia ethanolic extract","authors":"Anju A. Varghese, A. Smith A., S. M.","doi":"10.51248/.v43i3.2526","DOIUrl":"https://doi.org/10.51248/.v43i3.2526","url":null,"abstract":"Introduction and Aim: Crude herbal drugs and commercial extracts obtained from plant sources continue to play a significant role in healthcare, particularly in developing countries where traditional medicine practices are deeply rooted. The aim of the study was to estimate the antioxidant (DPPH) and cytotoxic effects of the ethanolic extract of Flaveria trinervia on raw 246.7 cells.\u0000 \u0000Materials and Methods: In the Indian town of Thoothukudi's Kovilpatti village, F. trinervia was isolated. The entire plant was air-dried before being crushed, and 1 kg of the powdered ethanolic extract was employed in the Soxhlet apparatus for continuous extraction. By utilizing GC-MS analysis, F. trinervia ethanolic extract phytochemical assessment was studied. The antioxidant potential of F. trinervia was examined using the DPPH test. Raw 246.7 cell lines were used to test the ethanolic extract of F. trinervia for cytotoxic activity.\u0000 \u0000Results: F. trinervia extracts were found to contain a variety of biologically active phytoconstituents, including ergosterol, octadecanoic acid, propanoic acid, and isopropyl palmitate, according to phytochemical analysis. Compared to the negative control, the ethanolic extract of F. trinervia considerably showed antioxidant activity and cytotoxicity. Ascorbic acid antioxidant activity with DPPH was 98.12%, whereas F.trinervia ethanolic extract's antioxidant activity was 78.91%. The anticancer efficacy of F. trinervia ethanolic extract against uncultured 246.7 cells was evaluated using the MTT assay. By charting cell viability vs extract concentration, the IC50 value was determined. The MTT assay identified raw cells IC50 at 24 hrs as 200 µg/mL of ethanolic F. trinervia extract.\u0000 \u0000Conclusion: Active phytoconstituents are present in the F. trinervia whole plant ethanolic extract. Because of the presence of phytoconstituents, the extracts have excellent antioxidant activity and good cytotoxicity activity against raw 246.7 cell lines. The study recommended using whole plant extract from F. trinervia to treat several metabolic diseases.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45205430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: In the global pandemic caused by Covid-19, older individuals and those with comorbidities have been shown to be more susceptible to serious diseases. The purpose of our research is to estimate the proportion of Covid-19 inpatients requiring blood transfusions over a period of 3 months. Materials and Methods: A retrospective observational analysis of blood product consumption in Covid-19 inpatients at our center from April to June 2021 was conducted by reviewing monthly transfusion records archived in our blood bank. Each blood component administered in Covid-19 wards during the prescribed period was noted. Results: Out of 1096 Covid-19 inpatients, only 66 (6.02%) patients received transfusions. As compared to non-Covid wards, Covid-19 wards had substantially lower transfusion rates for packed cell units (84 vs. 1074 units), FFP (33 vs. 691 units), and platelets (46 vs. 595 units). A total of 163 units of blood components were given to 66 Covid-19 inpatients, the majority of which were packed cell units (51.5%). Conclusion: In comparison to hospitalized non Covid patients, Covid-19 inpatients required fewer transfusions. In our institute, only the Covid 19 patients who were critically sick received blood transfusions; the rest received conservative therapy for their mild to moderate illnesses. This information will be beneficial for our department as it helps us plan and prepare for the use of blood supplies while the pandemic is still going strong.
{"title":"Changing trends in blood component utilisation during COVID time: Analysis of 1096 COVID-19 in patients","authors":"Christy K. Jose, K. Pailoor","doi":"10.51248/.v43i3.1334","DOIUrl":"https://doi.org/10.51248/.v43i3.1334","url":null,"abstract":"Introduction and Aim: In the global pandemic caused by Covid-19, older individuals and those with comorbidities have been shown to be more susceptible to serious diseases. The purpose of our research is to estimate the proportion of Covid-19 inpatients requiring blood transfusions over a period of 3 months.\u0000 \u0000Materials and Methods: A retrospective observational analysis of blood product consumption in Covid-19 inpatients at our center from April to June 2021 was conducted by reviewing monthly transfusion records archived in our blood bank. Each blood component administered in Covid-19 wards during the prescribed period was noted.\u0000 \u0000Results: Out of 1096 Covid-19 inpatients, only 66 (6.02%) patients received transfusions. As compared to non-Covid wards, Covid-19 wards had substantially lower transfusion rates for packed cell units (84 vs. 1074 units), FFP (33 vs. 691 units), and platelets (46 vs. 595 units). A total of 163 units of blood components were given to 66 Covid-19 inpatients, the majority of which were packed cell units (51.5%).\u0000 \u0000Conclusion: In comparison to hospitalized non Covid patients, Covid-19 inpatients required fewer transfusions. In our institute, only the Covid 19 patients who were critically sick received blood transfusions; the rest received conservative therapy for their mild to moderate illnesses. This information will be beneficial for our department as it helps us plan and prepare for the use of blood supplies while the pandemic is still going strong.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43293580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saja Mahir Mohammed, Zainab Hassan Hashim, Qasim Sh. Al-Mayah, Mahir Mohammed Hussein
Introduction and Aim: The zinc finger protein 804A (ZNF804A) gene has garnered significant interest as a gene associated with an increased risk of psychosis. This interest stems from the strong association observed between a specific single nucleotide polymorphism (SNP), namely rs1344706, within the ZNF804A gene, and various psychosis phenotypes, including schizophrenia and bipolar disorder. In this study, we aimed to assess the functional impact of ZNF804A gene polymorphism in individuals diagnosed with schizophrenia and methamphetamine addiction. Materials and Methods: This nested case control research included 50 individuals with schizophrenia and 50 methamphetamine (crystal) users recruited from Ibn-Rushed Psychiatric Teaching Hospital in Baghdad. 50 healthy subjects were also recruited as a control group. All participants were subjected to thorough physical examination. The serum concentration of Zinc Finger Protein-804A (ZNF804A) was determined using a sandwich enzyme-linked immunosorbent assay. Genomic DNA was isolated from whole blood samples, and the Taqman allelic discrimination method was used to genotype the ZNF804A gene rs1344706 polymorphism. Results: Patients with schizophrenia had a significantly higher median level of ZNF804A (48.67 ng/L) than controls (25.56 ng/L) or methamphetamine users (35.62 ng/L). The SNP rs1344706 homozygous (AA) genotype was more common in schizophrenia patients (36% vs. 16%) and methamphetamine addiction patients (4%), with significant differences (OR= 1.32, 95%CI= 1.32-11.75, p=0.014 and OR= 11.7, 95%CI=02.43-56.4, p=0.002, respectively). Conclusion: The AA genotype of rs1344706 polymorphism could be considered a risk factor for schizophrenia but has a protective role against methamphetamine addiction. Serum level of ZNF804A could be used to confirm the diagnosis of schizophrenia in young individuals with subtle clinical features.
{"title":"Assessment of the role of zinc finger protein-804a (znf804a) (rs1344706) gene polymorphism in schizophrenia and methamphetamine addiction patients","authors":"Saja Mahir Mohammed, Zainab Hassan Hashim, Qasim Sh. Al-Mayah, Mahir Mohammed Hussein","doi":"10.51248/.v43i3.2819","DOIUrl":"https://doi.org/10.51248/.v43i3.2819","url":null,"abstract":"Introduction and Aim: The zinc finger protein 804A (ZNF804A) gene has garnered significant interest as a gene associated with an increased risk of psychosis. This interest stems from the strong association observed between a specific single nucleotide polymorphism (SNP), namely rs1344706, within the ZNF804A gene, and various psychosis phenotypes, including schizophrenia and bipolar disorder. In this study, we aimed to assess the functional impact of ZNF804A gene polymorphism in individuals diagnosed with schizophrenia and methamphetamine addiction.\u0000 \u0000Materials and Methods: This nested case control research included 50 individuals with schizophrenia and 50 methamphetamine (crystal) users recruited from Ibn-Rushed Psychiatric Teaching Hospital in Baghdad. 50 healthy subjects were also recruited as a control group. All participants were subjected to thorough physical examination. The serum concentration of Zinc Finger Protein-804A (ZNF804A) was determined using a sandwich enzyme-linked immunosorbent assay. Genomic DNA was isolated from whole blood samples, and the Taqman allelic discrimination method was used to genotype the ZNF804A gene rs1344706 polymorphism.\u0000 \u0000Results: Patients with schizophrenia had a significantly higher median level of ZNF804A (48.67 ng/L) than controls (25.56 ng/L) or methamphetamine users (35.62 ng/L). The SNP rs1344706 homozygous (AA) genotype was more common in schizophrenia patients (36% vs. 16%) and methamphetamine addiction patients (4%), with significant differences (OR= 1.32, 95%CI= 1.32-11.75, p=0.014 and OR= 11.7, 95%CI=02.43-56.4, p=0.002, respectively).\u0000 \u0000Conclusion: The AA genotype of rs1344706 polymorphism could be considered a risk factor for schizophrenia but has a protective role against methamphetamine addiction. Serum level of ZNF804A could be used to confirm the diagnosis of schizophrenia in young individuals with subtle clinical features. ","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48721861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: In developed countries, carotid artery stenosis (CAS) has a considerable impact on mortality and disability rates. Genetic risk factors for CAS have also been linked, in addition to environmental risk factors. This study sought to determine whether there may be a link between three polymorphisms in the LPL gene and the emergence of CAS. Materials and Methods: One hundred and twenty participants were enrolled in this case-control study, including 60 individuals with CAS and 60 healthy subjects serving as the control group. The demographic and clinical data were collected from each participant. Whole blood samples were obtained to study the genomic DNA where a specific LPL gene fragment corresponding to the three single nucleotide polymorphisms (SNPs) rs320, rs328, and rs285 was amplified using designated primers. Restriction fragment length polymorphism analysis was used for genotyping. Results: The mutant allele (C) of the rs285 polymorphism was more frequent in patients than controls (45% vs. 32.5%; OR=1.7, 95% CI= 1.01- 2.87; p=0.048). The TCC haplotype block (T allele of rs320, C allele of rs285, and C allele of rs328) was significantly more prevalent in patients compared to controls (OR= 2.0, 95% CI= 1.03-3.77, p= 0.039). On the other hand, controls (23.33%) had significantly more of the haplotype block GTG than did patients (8.33%) (OR = 0.3, 95% CI = 0.14-0.065, p = 0.002). The SNPs rs320 (D' = 0.63) and rs328 (D' = 0.61) have weak relationships with rs385. Conclusion: The C allele of rs285 polymorphism could be considered a risk factor for CAS. While the haplotype block GTG was thought to play a protective role, the haplotype block TCC (T allele of rs320, C allele of rs285, and C allele of rs328) may increase the risk of CAS.
{"title":"Lipoprotein lipase gene polymorphisms and their association with the development and severity of carotid artery stenosis","authors":"Mahmood Shaker Khazaal, Farqad Bader Hamdan, Qasim Sharhan Al-Mayah","doi":"10.51248/.v43i3.2818","DOIUrl":"https://doi.org/10.51248/.v43i3.2818","url":null,"abstract":"Introduction and Aim: In developed countries, carotid artery stenosis (CAS) has a considerable impact on mortality and disability rates. Genetic risk factors for CAS have also been linked, in addition to environmental risk factors. This study sought to determine whether there may be a link between three polymorphisms in the LPL gene and the emergence of CAS.\u0000 \u0000Materials and Methods: One hundred and twenty participants were enrolled in this case-control study, including 60 individuals with CAS and 60 healthy subjects serving as the control group. The demographic and clinical data were collected from each participant. Whole blood samples were obtained to study the genomic DNA where a specific LPL gene fragment corresponding to the three single nucleotide polymorphisms (SNPs) rs320, rs328, and rs285 was amplified using designated primers. Restriction fragment length polymorphism analysis was used for genotyping.\u0000 \u0000Results: The mutant allele (C) of the rs285 polymorphism was more frequent in patients than controls (45% vs. 32.5%; OR=1.7, 95% CI= 1.01- 2.87; p=0.048). The TCC haplotype block (T allele of rs320, C allele of rs285, and C allele of rs328) was significantly more prevalent in patients compared to controls (OR= 2.0, 95% CI= 1.03-3.77, p= 0.039). On the other hand, controls (23.33%) had significantly more of the haplotype block GTG than did patients (8.33%) (OR = 0.3, 95% CI = 0.14-0.065, p = 0.002). The SNPs rs320 (D' = 0.63) and rs328 (D' = 0.61) have weak relationships with rs385.\u0000 \u0000Conclusion: The C allele of rs285 polymorphism could be considered a risk factor for CAS. While the haplotype block GTG was thought to play a protective role, the haplotype block TCC (T allele of rs320, C allele of rs285, and C allele of rs328) may increase the risk of CAS.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49525582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reactive oxygen species (ROS) are the molecules which have oxygen an atom in its highly reactive form. ROS are formed as a byproduct of normal metabolism and are removed by the antioxidants and enzymes present in the system. But, uncontrolled formation of ROS due to various factors like diseases or stress causes damage to the biomolecules. This oxidation of biomolecules by ROS might help in the progression of human diseases. Understanding the mechanism of development of different human diseases caused due to ROS will help to control the severity of the diseases. Also, on the basis of types of ROS involved, an antioxidant supplement can be selected to mitigate the effect of ROS and ultimately lower the severity of the disease. In this review article, we have tried to summarize the literature available from different sources on ROS formation, biomolecule oxidation and their impact on the progression of various diseases in humans.
{"title":"Impact of reactive oxygen species on the progression of human diseases by damaging biomolecules","authors":"V. Pathak, R. Kant, Navneeta R. Kumar","doi":"10.51248/.v43i3.2439","DOIUrl":"https://doi.org/10.51248/.v43i3.2439","url":null,"abstract":"Reactive oxygen species (ROS) are the molecules which have oxygen an atom in its highly reactive form. ROS are formed as a byproduct of normal metabolism and are removed by the antioxidants and enzymes present in the system. But, uncontrolled formation of ROS due to various factors like diseases or stress causes damage to the biomolecules. This oxidation of biomolecules by ROS might help in the progression of human diseases. Understanding the mechanism of development of different human diseases caused due to ROS will help to control the severity of the diseases. Also, on the basis of types of ROS involved, an antioxidant supplement can be selected to mitigate the effect of ROS and ultimately lower the severity of the disease. In this review article, we have tried to summarize the literature available from different sources on ROS formation, biomolecule oxidation and their impact on the progression of various diseases in humans.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44651411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sathya Siva, Madhanraj Sekar, Surya Prakash V., Titus J., Mohammed Arifullah Mohamed Fazulah, S. V
Protease enzymes found in the Indian papaya, also called Carica papaya, are known to have de-sloughing and wound healing properties. It was found that Carica papaya applied to a lesion enhanced phagocytic cell killing of bacteria. Green papaya is rich in papain and chymopapain, two potent digestive enzymes that can break down dead tissue and have anti-inflammatory properties. To compare the effectiveness of conventional and Carica papaya management in wound healing the systematic review and meta-analysis is conducted. Based on PRISMA (preferred reporting items for systematic reviews and meta-analyses) guidelines, a systematic review is conducted. While using the search terms ‘Diabetic foot ulcer, conventional management, wound healing, Carica papaya’ in the MEDLINE, Google Scholar, and PubMed databases. Carica papaya has a good beneficial effect on reducing necrosis and is frequently utilized in the management of wound healing. None of the studies have demonstrated any adverse impacts of Carica papaya on wound healing.
{"title":"Effectiveness of conventional management and Carica papaya on influencing wound healing: A systematic review and meta-analysis","authors":"Sathya Siva, Madhanraj Sekar, Surya Prakash V., Titus J., Mohammed Arifullah Mohamed Fazulah, S. V","doi":"10.51248/.v43i3.2851","DOIUrl":"https://doi.org/10.51248/.v43i3.2851","url":null,"abstract":"Protease enzymes found in the Indian papaya, also called Carica papaya, are known to have de-sloughing and wound healing properties. It was found that Carica papaya applied to a lesion enhanced phagocytic cell killing of bacteria. Green papaya is rich in papain and chymopapain, two potent digestive enzymes that can break down dead tissue and have anti-inflammatory properties. To compare the effectiveness of conventional and Carica papaya management in wound healing the systematic review and meta-analysis is conducted. Based on PRISMA (preferred reporting items for systematic reviews and meta-analyses) guidelines, a systematic review is conducted. While using the search terms ‘Diabetic foot ulcer, conventional management, wound healing, Carica papaya’ in the MEDLINE, Google Scholar, and PubMed databases. Carica papaya has a good beneficial effect on reducing necrosis and is frequently utilized in the management of wound healing. None of the studies have demonstrated any adverse impacts of Carica papaya on wound healing.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46613814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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