Salah Aldeen Ahmed Naji Ismail, R. Mahmood, Haseebur Rahman
Introduction and Aim: Solanum pubescens is one of the very well-known traditional medicinal plants it has been widely used in the treatment of many inflammatory conditions. This study aimed to evaluate the anti-inflammatory property of S. pubescens fruit. Materials and Methods: Anti-inflammatory activities of S. pubescens fruit extracts at different concentrations (100-200 mg/kg) were carried out using carrageenan-induced and cotton implant-induced granuloma methods. Results: The results of the carrageenan-induced paw edema study reveals the highest activity of fruit upper ethanolic (FUEE) extract at 200 mg/kg, with 64.84% followed by water (FEW), and fruit lower ethanolic extracts (FLEE). The protein and IL-6 evaluation in this experiment showed very promising results. Whereas the cellular toxicity was evaluated in terms of LPO, MPO and nitric oxide analysis which in turn showed harmless nature of extracts. Furthermore, assessment of proliferative phase of inflammation was done in rats using a cotton pellet-induced granuloma model, where FUEE at 100 mg/kg showed 32.5±2.73 mg, 200 mg/kg 25.3±2.36 mg of dry weight of granuloma, compared to the standard indomethacin activity 1.4±3.45 mg. Moreover, the hematological parameters of treated animals reveals that the erythrocyte sedimentation rate (ESR) of FUEE 4.88± 0.18 mm/hr at 200 mg/kg was the least and almost equal to the standard. Conclusion: It can be said that S. pubescens is no doubt a very good source of phytomedicine for the treatment of inflammatory diseases, with most of the valuable phytoconstituents concerned with the anti-inflammation assembled in the fruit upper ethanolic extracts as this extract showed excellent activity in both the carrageenan and cotton pellet models.
{"title":"Evaluation of the anti-inflammatory potential of Solanum pubescens fruit extract","authors":"Salah Aldeen Ahmed Naji Ismail, R. Mahmood, Haseebur Rahman","doi":"10.51248/.v43i02.2687","DOIUrl":"https://doi.org/10.51248/.v43i02.2687","url":null,"abstract":"Introduction and Aim: Solanum pubescens is one of the very well-known traditional medicinal plants it has been widely used in the treatment of many inflammatory conditions. This study aimed to evaluate the anti-inflammatory property of S. pubescens fruit.\u0000 \u0000Materials and Methods: Anti-inflammatory activities of S. pubescens fruit extracts at different concentrations (100-200 mg/kg) were carried out using carrageenan-induced and cotton implant-induced granuloma methods.\u0000 \u0000Results: The results of the carrageenan-induced paw edema study reveals the highest activity of fruit upper ethanolic (FUEE) extract at 200 mg/kg, with 64.84% followed by water (FEW), and fruit lower ethanolic extracts (FLEE). The protein and IL-6 evaluation in this experiment showed very promising results. Whereas the cellular toxicity was evaluated in terms of LPO, MPO and nitric oxide analysis which in turn showed harmless nature of extracts. Furthermore, assessment of proliferative phase of inflammation was done in rats using a cotton pellet-induced granuloma model, where FUEE at 100 mg/kg showed 32.5±2.73 mg, 200 mg/kg 25.3±2.36 mg of dry weight of granuloma, compared to the standard indomethacin activity 1.4±3.45 mg. Moreover, the hematological parameters of treated animals reveals that the erythrocyte sedimentation rate (ESR) of FUEE 4.88± 0.18 mm/hr at 200 mg/kg was the least and almost equal to the standard.\u0000 \u0000Conclusion: It can be said that S. pubescens is no doubt a very good source of phytomedicine for the treatment of inflammatory diseases, with most of the valuable phytoconstituents concerned with the anti-inflammation assembled in the fruit upper ethanolic extracts as this extract showed excellent activity in both the carrageenan and cotton pellet models.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42708793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Gaucher disease (GD) is an autosomal recessive ailment caused due to mutations in the GBA1 gene, encoding for the lysosomal enzyme, glucocerebrosidase. The aim was to evaluate the clinical, biochemical, and molecular parameters associated with this disease, as well as to identify symptoms and covariables thought to be most diagnostic of early GD presentation, allowing for early diagnosis and management. Methodology: This cross-sectional study involved twenty-six patients diagnosed with GD at the Metabolic Department of Central Child Teaching Hospital, Baghdad, Iraq. Diagnosis depended mainly on history and physical examination and confirmed by beta-glucosidase enzyme assay of dry blood spot on filter paper and Lyso-GL-1 level. Amplification-based next generation sequencing approach was used in investigating the GBA1 gene at the molecular level. Results: The mean age of the 26 (17 male and 9 female) patients was 9.13 years, with 100% consanguinity and 50% positive family history. The average number of years from the start of clinical manifestations to the diagnosis' validation was 3.82 years. The original age of presentation was 2.83 years, and the initial age of diagnosis was 6.65 years. Hepatosplenomegaly (85%), pallor (88%), splenomegaly (12%), splenectomy (12%), hemorrhage (19%), bone discomfort (23%), bone breakage (12%), and GD type III (19%) were observed. Among patients 65% exhibited radiological bone abnormalities, 54% had Erlenmeyer flask deformity, and 1.5% had osteopenia and fracture. Studies of GBA1 gene showed the mutation C.[1448T>c ](P.[ Leu483Pro] to be the most common. Hepatosplenomegaly and hematological deficiencies were effectively improved by enzyme replacement therapy. Conclusion: In Gaucher disease, GBA1 gene mutation analysis could provide some predictive information about disease variance as well as severity. PCV%, platelet count, ferritin, and lyso-GL-1 levels could be employed as biomarkers for the diagnosis of GD. ERT proved effective in treating hematological and hepatosplenomegaly abnormalities.
{"title":"A reappraisal of Gaucher disease patients - Clinical presentation, and diagnosis in rare disease unit of central child teaching hospital in Baghdad province","authors":"Ikhlas Ali Ahmed, Alaa Abbas Fadhel","doi":"10.51248/.v43i02.2700","DOIUrl":"https://doi.org/10.51248/.v43i02.2700","url":null,"abstract":"Introduction and Aim: Gaucher disease (GD) is an autosomal recessive ailment caused due to mutations in the GBA1 gene, encoding for the lysosomal enzyme, glucocerebrosidase. The aim was to evaluate the clinical, biochemical, and molecular parameters associated with this disease, as well as to identify symptoms and covariables thought to be most diagnostic of early GD presentation, allowing for early diagnosis and management.\u0000 \u0000Methodology: This cross-sectional study involved twenty-six patients diagnosed with GD at the Metabolic Department of Central Child Teaching Hospital, Baghdad, Iraq. Diagnosis depended mainly on history and physical examination and confirmed by beta-glucosidase enzyme assay of dry blood spot on filter paper and Lyso-GL-1 level. Amplification-based next generation sequencing approach was used in investigating the GBA1 gene at the molecular level.\u0000 \u0000Results: The mean age of the 26 (17 male and 9 female) patients was 9.13 years, with 100% consanguinity and 50% positive family history. The average number of years from the start of clinical manifestations to the diagnosis' validation was 3.82 years. The original age of presentation was 2.83 years, and the initial age of diagnosis was 6.65 years. Hepatosplenomegaly (85%), pallor (88%), splenomegaly (12%), splenectomy (12%), hemorrhage (19%), bone discomfort (23%), bone breakage (12%), and GD type III (19%) were observed. Among patients 65% exhibited radiological bone abnormalities, 54% had Erlenmeyer flask deformity, and 1.5% had osteopenia and fracture. Studies of GBA1 gene showed the mutation C.[1448T>c ](P.[ Leu483Pro] to be the most common. Hepatosplenomegaly and hematological deficiencies were effectively improved by enzyme replacement therapy.\u0000 \u0000Conclusion: In Gaucher disease, GBA1 gene mutation analysis could provide some predictive information about disease variance as well as severity. PCV%, platelet count, ferritin, and lyso-GL-1 levels could be employed as biomarkers for the diagnosis of GD. ERT proved effective in treating hematological and hepatosplenomegaly abnormalities.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44142048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Fenugreek (Trigonella foenum graceum) is extensively cultivated in several Asian nations. The leaves and seeds of this plant are well recognized for their potential against diabetes, some cancers and imparting immunity. They are often employed as adhesives and emulsifying agents. Polysaccharides can be found in abundance in fenugreek seeds. Galactomannan, a water-soluble polysaccharide, efficiently reduces the surface tension and increases the viscosity of liquids. Materials and Methods: In this study, a simple method for extracting as well as purifying galactomannan was developed. It was produced from fenugreek plant's seeds and purified by centrifugation and isopropyl alcohol (IPA) spirit precipitation methods. Results: The presence of carbohydrates by Molisch test and an absence of reducing sugar by Fehling’s test was revealed. The pH of the purified galactomannan was 6.37, and its foaming capability was 14.28 %. The Galactomannan gum was found to possess 81 % emulsifying capacity. It revealed that the capacity to hold water was 1480% and the ability to hold oil was 268%. Conclusion: In contrast to guar gum as well as the locust bean gum which are employed in various industries as thickeners, emulsifiers and stabilizers, galactomannan, produced in this study, is an effective and affordable method of stabilizing and emulsifying various products.
{"title":"Extraction, purification and assessment of galactomannan from fenugreek seeds","authors":"Jahnavi Teekanam, Shantkriti Srinivasan, Pavithra Uthayasooriyan, Usharani Subbiah, B. Govindasamy, Murugan Athiappan","doi":"10.51248/.v43i02.2545","DOIUrl":"https://doi.org/10.51248/.v43i02.2545","url":null,"abstract":"Introduction and Aim: Fenugreek (Trigonella foenum graceum) is extensively cultivated in several Asian nations. The leaves and seeds of this plant are well recognized for their potential against diabetes, some cancers and imparting immunity. They are often employed as adhesives and emulsifying agents. Polysaccharides can be found in abundance in fenugreek seeds. Galactomannan, a water-soluble polysaccharide, efficiently reduces the surface tension and increases the viscosity of liquids.\u0000 \u0000Materials and Methods: In this study, a simple method for extracting as well as purifying galactomannan was developed. It was produced from fenugreek plant's seeds and purified by centrifugation and isopropyl alcohol (IPA) spirit precipitation methods.\u0000 \u0000Results: The presence of carbohydrates by Molisch test and an absence of reducing sugar by Fehling’s test was revealed. The pH of the purified galactomannan was 6.37, and its foaming capability was 14.28 %. The Galactomannan gum was found to possess 81 % emulsifying capacity. It revealed that the capacity to hold water was 1480% and the ability to hold oil was 268%.\u0000 \u0000Conclusion: In contrast to guar gum as well as the locust bean gum which are employed in various industries as thickeners, emulsifiers and stabilizers, galactomannan, produced in this study, is an effective and affordable method of stabilizing and emulsifying various products.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70666305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rajkumar S. Meti, Neelagund S. E., Deepadarshan Urs, Dharmappa K. K., Kotresh K. R.
Introduction and Aim: Worldwide, mosquitoes are the main vectors of many deadly diseases like malaria, dengue, chikungunya, etc., Anopheles stephensi mosquito which transmits malaria whereas dengue is transmitted mainly by mosquito Aedes aegypti. Current controlling methods such as chemical or microbial pesticides, repellents, biological control agents against mosquito larvae are not so effective. The leading cause of death worldwide is cancer. Nanotechnology can provide alternative effective methods for malaria, dengue and cancer control, diagnosis and treatment. This study investigated the biosynthesis of gold nanoparticles (AuNPs) from Acacia sinuata seed extract and their mosquito larvicidal potential was tested against Aedes aegypti, Anopheles stephensi larvae. The cytotoxic activity of NPs was also analyzed against human cancer cell lines osteosarcoma (MG-63) and colon adenocarcinoma (Caco-2). Materials and Methods: The biosynthesized NPs were confirmed and characterized by conventional techniques UV- visible spectroscopy, FTIR, XRD, HR-TEM, EDX and SAED. Results: The XRD demonstrated the NPs were face-centred, cubic, and crystalline in nature, EDX study confirmed elemental analysis of gold, SAED illustrated the crystalline nature. The HR-TEM studies revealed NPs shape which were mostly spherical and average size of 5.38nm-8.86nm. Third instar larvae of Aedes aegypti, Anopheles stephensi, were susceptible to the larvicidal effects of AuNPs. The synthesized NPs showed a dose-dependent cytotoxic effect against the Caco-2 and MG-63 cell lines, with IC50 (inhibitory concentrations) of 21.31± 0.15 ug/ml and 86.78± 0.23 ug/ml, respectively. Conclusion: These findings reveal that biosynthesized AuNPs have substantial larvicidal and anticancer properties, suggesting they could be used in mosquito control and cancer treatment.
{"title":"Biosynthesis, mosquito larvicidal potential, and anticancer activities of gold nanoparticles from Acacia sinuata seed extract","authors":"Rajkumar S. Meti, Neelagund S. E., Deepadarshan Urs, Dharmappa K. K., Kotresh K. R.","doi":"10.51248/.v43i02.2706","DOIUrl":"https://doi.org/10.51248/.v43i02.2706","url":null,"abstract":"Introduction and Aim: Worldwide, mosquitoes are the main vectors of many deadly diseases like malaria, dengue, chikungunya, etc., Anopheles stephensi mosquito which transmits malaria whereas dengue is transmitted mainly by mosquito Aedes aegypti. Current controlling methods such as chemical or microbial pesticides, repellents, biological control agents against mosquito larvae are not so effective. The leading cause of death worldwide is cancer. Nanotechnology can provide alternative effective methods for malaria, dengue and cancer control, diagnosis and treatment. This study investigated the biosynthesis of gold nanoparticles (AuNPs) from Acacia sinuata seed extract and their mosquito larvicidal potential was tested against Aedes aegypti, Anopheles stephensi larvae. The cytotoxic activity of NPs was also analyzed against human cancer cell lines osteosarcoma (MG-63) and colon adenocarcinoma (Caco-2).\u0000 \u0000Materials and Methods: The biosynthesized NPs were confirmed and characterized by conventional techniques UV- visible spectroscopy, FTIR, XRD, HR-TEM, EDX and SAED.\u0000 \u0000Results: The XRD demonstrated the NPs were face-centred, cubic, and crystalline in nature, EDX study confirmed elemental analysis of gold, SAED illustrated the crystalline nature. The HR-TEM studies revealed NPs shape which were mostly spherical and average size of 5.38nm-8.86nm. Third instar larvae of Aedes aegypti, Anopheles stephensi, were susceptible to the larvicidal effects of AuNPs. The synthesized NPs showed a dose-dependent cytotoxic effect against the Caco-2 and MG-63 cell lines, with IC50 (inhibitory concentrations) of 21.31± 0.15 ug/ml and 86.78± 0.23 ug/ml, respectively.\u0000 \u0000Conclusion: These findings reveal that biosynthesized AuNPs have substantial larvicidal and anticancer properties, suggesting they could be used in mosquito control and cancer treatment.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42279260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kalesh M. Karun, Amitha Puranik, Lintu M. K., D. M. S.
Introduction and Aim: Pneumonia is a common and serious illness among the elderly. Early identification of the risk factors for pneumonia is essential for improving the survival outcomes among elderly. The present study aimed to identify an optimal regression approach to determine the risk factors for pneumonia among elderly patients. Materials and Methods: The present study utilized data from the Medical Information Mart for Intensive Care (MIMIC III) to evaluate the use of alternative generalized linear models to identify the risk factors for pneumonia. The regression model with the smallest AIC, BIC and SE was considered as the appropriate regression model for the data. Robust Poisson model was considered the best fit for the current data as it had the lowest AIC, BIC and standard error compared to other regression models. Results: Variables such as BMI, renal failure, hypertension, diabetes and asthma were identified as the significant risk factors for pneumonia. The risk of pneumonia was found to be significantly higher in the underweight category of BMI [RRadj=1.70; 95% CI=1.38, 2.08]; diabetic patients [RRadj =1.29; 95% CI=1.03, 1.61); asthmatic patients [RRadj =1.35; 95% CI=1.15, 1.58] and patients with renal failure [RRadj =1.16; 95% CI= 1.05, 1.29]. Conclusion: Among various binary regression models, Poisson regression with robust variance (sandwich Poisson regression) provided unbiased estimates of the relationship. In the present study, variables such as BMI, renal failure, diabetics, hypertension and asthma were identified as the significant risk factors for pneumonia in the elderly using robust Poisson regression.
{"title":"Risk factors of pneumonia among elderly with robust Poisson regression - A study on mimic III data","authors":"Kalesh M. Karun, Amitha Puranik, Lintu M. K., D. M. S.","doi":"10.51248/.v43i02.2250","DOIUrl":"https://doi.org/10.51248/.v43i02.2250","url":null,"abstract":"Introduction and Aim: Pneumonia is a common and serious illness among the elderly. Early identification of the risk factors for pneumonia is essential for improving the survival outcomes among elderly. The present study aimed to identify an optimal regression approach to determine the risk factors for pneumonia among elderly patients.\u0000 \u0000Materials and Methods: The present study utilized data from the Medical Information Mart for Intensive Care (MIMIC III) to evaluate the use of alternative generalized linear models to identify the risk factors for pneumonia. The regression model with the smallest AIC, BIC and SE was considered as the appropriate regression model for the data. Robust Poisson model was considered the best fit for the current data as it had the lowest AIC, BIC and standard error compared to other regression models.\u0000 \u0000Results: Variables such as BMI, renal failure, hypertension, diabetes and asthma were identified as the significant risk factors for pneumonia. The risk of pneumonia was found to be significantly higher in the underweight category of BMI [RRadj=1.70; 95% CI=1.38, 2.08]; diabetic patients [RRadj =1.29; 95% CI=1.03, 1.61); asthmatic patients [RRadj =1.35; 95% CI=1.15, 1.58] and patients with renal failure [RRadj =1.16; 95% CI= 1.05, 1.29].\u0000 \u0000Conclusion: Among various binary regression models, Poisson regression with robust variance (sandwich Poisson regression) provided unbiased estimates of the relationship. In the present study, variables such as BMI, renal failure, diabetics, hypertension and asthma were identified as the significant risk factors for pneumonia in the elderly using robust Poisson regression.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44497380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Balasubramaniyam, Grrishma Balakrishnan, Padmini Thalenjeri, V. Rao
Introduction and Aim: Anemia is common among women within childbearing age. The present study was undertaken to analyze the burden ofsocio-demographic status, lifestyle determinants, menstrual parameters, and obstetric factors as risk factors among mild, moderate, and severe types of anaemic women of childbearing age and to assess their knowledge about it. Methods: A cross-sectional study was done among 79 anemic women visiting a private medical college hospital in Karnataka. The study group was categorized into mild, moderate, and severe anemia based on the WHO criteria of haemoglobin concentration. Participants answered a validated and pretested questionnaire on their socio-demographic status, lifestyle characteristics, menstrual and obstetric history, and their knowledge regarding anemia. Results: Descriptive statistics were used for the data analysis. The prevalence of mild and moderate anemia in the rural setup was higher compared to urban living. Most of the participants with mild to moderate anemia had education up to primary schooling and were married homemakers. Participants with moderate to severe anemia belonged to the below poverty line status. There was a higher intake of white meat among moderate anemic participants compared to the other groups.Majority of the participants with mild to moderate anemia suffered from dysmenorrhoea. Only 51% of the participants knew that anemia is a health problem and most of the participants were ignorant of the features of anemia. Conclusion: We conclude that moderately anemic participants mostly belonged to rural areas, were less literate, belonging to lower economic strata, consuming white meat and junk food.
{"title":"A descriptive study of myriad risk factors in anemia of varied severity among reproductive female patients visiting a tertiary care setup in coastal Karnataka","authors":"K. Balasubramaniyam, Grrishma Balakrishnan, Padmini Thalenjeri, V. Rao","doi":"10.51248/.v43i02.2905","DOIUrl":"https://doi.org/10.51248/.v43i02.2905","url":null,"abstract":"Introduction and Aim: Anemia is common among women within childbearing age. The present study was undertaken to analyze the burden ofsocio-demographic status, lifestyle determinants, menstrual parameters, and obstetric factors as risk factors among mild, moderate, and severe types of anaemic women of childbearing age and to assess their knowledge about it. \u0000 \u0000Methods: A cross-sectional study was done among 79 anemic women visiting a private medical college hospital in Karnataka. The study group was categorized into mild, moderate, and severe anemia based on the WHO criteria of haemoglobin concentration. Participants answered a validated and pretested questionnaire on their socio-demographic status, lifestyle characteristics, menstrual and obstetric history, and their knowledge regarding anemia. \u0000 \u0000Results: Descriptive statistics were used for the data analysis. The prevalence of mild and moderate anemia in the rural setup was higher compared to urban living. Most of the participants with mild to moderate anemia had education up to primary schooling and were married homemakers. Participants with moderate to severe anemia belonged to the below poverty line status. There was a higher intake of white meat among moderate anemic participants compared to the other groups.Majority of the participants with mild to moderate anemia suffered from dysmenorrhoea. Only 51% of the participants knew that anemia is a health problem and most of the participants were ignorant of the features of anemia. \u0000 \u0000Conclusion: We conclude that moderately anemic participants mostly belonged to rural areas, were less literate, belonging to lower economic strata, consuming white meat and junk food.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49536881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Transgender healthcare has emerged as an area of medicine that has been in the blind spot of most physicians for the last century. Medical professionals in India have been equally unaware of the health needs of the transgender community. The aim of the present study was to assess the competency and awareness regarding transgender health and related issues among medical professionals. Methodology: A questionnaire was framed to assess awareness on social and medical issues faced by the transgender community containing 40 questions in yes or no format. 126 doctors filled the questionnaires and 58 of the respondents later attended a conference on Transgender health. Similar questionnaire was used to assess the improvement in knowledge after the conference. Results: The result of the questionnaire score was analysed in percentage. The average percentage score of the initial 126 respondents was 51.58. Pre-conference percentage score was 51.33 and the post-conference was 61.67 among the 58 conference attendees, showing a significant increase in awareness after being exposed to an educational session. Conclusion: There is a need for large-scale awareness programs and the implementation of trans-inclusive medical curriculum to improve the competency of medical professionals.
{"title":"Competency on transgender health issues among medical professionals before and after the medical conference on transgender physiology","authors":"D. R, A. Menon, N. George","doi":"10.51248/.v43i02.2479","DOIUrl":"https://doi.org/10.51248/.v43i02.2479","url":null,"abstract":"Introduction and Aim: Transgender healthcare has emerged as an area of medicine that has been in the blind spot of most physicians for the last century. Medical professionals in India have been equally unaware of the health needs of the transgender community. The aim of the present study was to assess the competency and awareness regarding transgender health and related issues among medical professionals.\u0000 \u0000Methodology: A questionnaire was framed to assess awareness on social and medical issues faced by the transgender community containing 40 questions in yes or no format. 126 doctors filled the questionnaires and 58 of the respondents later attended a conference on Transgender health. Similar questionnaire was used to assess the improvement in knowledge after the conference.\u0000 \u0000Results: The result of the questionnaire score was analysed in percentage. The average percentage score of the initial 126 respondents was 51.58. Pre-conference percentage score was 51.33 and the post-conference was 61.67 among the 58 conference attendees, showing a significant increase in awareness after being exposed to an educational session. \u0000 \u0000Conclusion: There is a need for large-scale awareness programs and the implementation of trans-inclusive medical curriculum to improve the competency of medical professionals.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44000629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Fine needle aspiration cytology (FNAC), is a frequently employed diagnostic procedure in identifying lymph node pathology. This study aims to categorise cytological aspirates from lymph nodes according to the Sydney system and in addition determine the likelihood of cancer occurrence in the classified categories. Materials and Methods: A cross-sectional study analysing lymph node cytology was performed retrospectively between January 2021 to December 2022, and the findings were classified into five groups from L1 to L5 as proposed by the Sydney System. By comparing the diagnoses with the corresponding histological diagnosis, statistical analysis was used to evaluate the probability of malignancy linked with each group. Results: Out of 279 fine needle aspiration cytology (FNAC) tests performed for lymphadenopathy, 39 cases were compared with histopathological results. The cases classified to the categories L1, L2, L3, L4, and L5 were 11/279 (3.9%), 170/279 (60%), 2/279 (0.7%), and 93/279 (33%) accordingly. The likelihood of developing cancer was found to be 33.33%, 8.8%, 56.4%, 83.33%, and 94.74% for each group. Conclusion: To achieve uniformity and repeatability in cytopathological diagnosis, the recommended Sydney method of lymph node cytology reporting and classification can be effective.
{"title":"An approach to classification and reporting lymph node cytopathology using Sydney system and evaluating the likelihood of malignancy","authors":"Ankita Shibu Robert, Crysle S Saldanha","doi":"10.51248/.v43i02.2517","DOIUrl":"https://doi.org/10.51248/.v43i02.2517","url":null,"abstract":"Introduction and Aim: Fine needle aspiration cytology (FNAC), is a frequently employed diagnostic procedure in identifying lymph node pathology. This study aims to categorise cytological aspirates from lymph nodes according to the Sydney system and in addition determine the likelihood of cancer occurrence in the classified categories.\u0000 \u0000Materials and Methods: A cross-sectional study analysing lymph node cytology was performed retrospectively between January 2021 to December 2022, and the findings were classified into five groups from L1 to L5 as proposed by the Sydney System. By comparing the diagnoses with the corresponding histological diagnosis, statistical analysis was used to evaluate the probability of malignancy linked with each group.\u0000 \u0000Results: Out of 279 fine needle aspiration cytology (FNAC) tests performed for lymphadenopathy, 39 cases were compared with histopathological results. The cases classified to the categories L1, L2, L3, L4, and L5 were 11/279 (3.9%), 170/279 (60%), 2/279 (0.7%), and 93/279 (33%) accordingly. The likelihood of developing cancer was found to be 33.33%, 8.8%, 56.4%, 83.33%, and 94.74% for each group.\u0000 \u0000Conclusion: To achieve uniformity and repeatability in cytopathological diagnosis, the recommended Sydney method of lymph node cytology reporting and classification can be effective.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43157664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Type 2 Diabetes Mellitus (T2DM) is presently the commonest and most prevalent disorder of metabolism which, if unmanaged, can lead to macro and micro-vascular disorders as complications. The preventive and therapeutic options for the same have been drastically improved than before and the life expectancy of the affected population has risen, but with the emergence of few other new complications like cognitive impairment and dementia. Insulin Resistance (IR) is a preclinical stage during diabetes and can potentially affect cognition. Cognition is the capability of an individual to process the given information through perception. Cognition is a broad spectrum including different cognitive domains like learning, attention, memory, language, reasoning, decision making, visuospatial skills etc., which forms the basis of intellectual development. Our present aim is to study the relationship between the glycemic profile and cognition status in diabetics. Materials and Methods: The study population included a total of 232 subjects with the age of 40-70 years of both genders. They were recruited after obtaining the informed written consent. Fasting blood glucose, Insulin levels, HbA1C were analyzed. The insulin resistance indices such as The HOmeostasis Model of IR (HOMA-IR), QUantitative Insulin-sensitivity Check Index (QUICKI) and HOMA percent beta-cell function (HOMA-beta) were derived. Cognition status was assessed and scored using the Modified Mini Mental Status (3MS) test. Results: HbA1c score and the 3MS score showed an association where both were negatively correlated. Insulin resistance induces too were negatively correlated with cognitive function. Conclusion: Thus, our study suggests that unmanaged diabetes mellitus type 2 may affect the cognition. Accordingly, early diagnosis of the condition and its management is crucial to bring down the incidence of cognitive impairment, further dementia, and other neurodegenerative diseases.
{"title":"Role of glycemic status and insulin resistance indices on cognition","authors":"R. C. M., R. R, Uma Vijayashankar, B. M","doi":"10.51248/.v43i02.2235","DOIUrl":"https://doi.org/10.51248/.v43i02.2235","url":null,"abstract":"Introduction and Aim: Type 2 Diabetes Mellitus (T2DM) is presently the commonest and most prevalent disorder of metabolism which, if unmanaged, can lead to macro and micro-vascular disorders as complications. The preventive and therapeutic options for the same have been drastically improved than before and the life expectancy of the affected population has risen, but with the emergence of few other new complications like cognitive impairment and dementia. Insulin Resistance (IR) is a preclinical stage during diabetes and can potentially affect cognition. Cognition is the capability of an individual to process the given information through perception. Cognition is a broad spectrum including different cognitive domains like learning, attention, memory, language, reasoning, decision making, visuospatial skills etc., which forms the basis of intellectual development. Our present aim is to study the relationship between the glycemic profile and cognition status in diabetics.\u0000 \u0000Materials and Methods: The study population included a total of 232 subjects with the age of 40-70 years of both genders. They were recruited after obtaining the informed written consent. Fasting blood glucose, Insulin levels, HbA1C were analyzed. The insulin resistance indices such as The HOmeostasis Model of IR (HOMA-IR), QUantitative Insulin-sensitivity Check Index (QUICKI) and HOMA percent beta-cell function (HOMA-beta) were derived. Cognition status was assessed and scored using the Modified Mini Mental Status (3MS) test.\u0000 \u0000Results: HbA1c score and the 3MS score showed an association where both were negatively correlated. Insulin resistance induces too were negatively correlated with cognitive function.\u0000 \u0000Conclusion: Thus, our study suggests that unmanaged diabetes mellitus type 2 may affect the cognition. Accordingly, early diagnosis of the condition and its management is crucial to bring down the incidence of cognitive impairment, further dementia, and other neurodegenerative diseases.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47538384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asif P. P., Rouchelle C. Tellis, Amar Sunil Lobo, A. Motagi
Introduction and Aim: This is a retrospective study to analyse the pathogens causing bacteraemia in COVID-19 patients and its correlation with inflammatory biomarkers such as procalcitonin (PCT), C-reactive protein (CRP), total WBC counts (TC) and D-dimer. The objectives of this study were to evaluate the blood culture positivity rate, to identify the pathogens causing bacteraemia, to analyse their antibiogram and to assess the significance of inflammatory markers along with patient outcomes. Materials and Methods: This study included 165 COVID-19-positive patients admitted to the Intensive care unit (ICU) of a tertiary care hospital from June to September 2020. Blood culture, identification and antibiotic sensitivity testing (AST) were done using automated systems. Values of D-dimer, CRP, PCT and TC were obtained using immunoturbidimetric assay, chemiluminescent immunoassay, immunochromatographic testing and automated haematology analyser respectively. Results: Among 143 blood culture samples obtained from 122 COVID-19 positive patients, 80 flagged positive. Out of the 80 isolates obtained, 53 (66.25%), 17 (21.25%) and 10 (12.5%) were gram-positive bacteria, gram-negative bacteria and candida respectively. The blood culture positivity rate was 25.4%. Acinetobacter spp. and K. pneumoniae showed high levels of antibiotic resistance. Among 16 patients with elevated PCT, 15 (93.7%) patients showed positive blood cultures. CRP of >5 mg/L and deranged total WBC counts were seen among all blood culture-positive patients. In 100 patients with elevated D-dimer, 54% (54/100) patients showed positive blood culture. Conclusion: From this study, we conclude that early identification of pathogens and initiation of appropriate antimicrobial therapy is crucial for managing sepsis associated with COVID-19 infection. PCT, CRP, TC and D-dimer can help as biomarkers in the management of COVID-19 patients with secondary bloodstream infections (BSI).
{"title":"Blood culture and profile of inflammatory biomarkers among COVID-19 patients in an intensive care unit of a tertiary care hospital","authors":"Asif P. P., Rouchelle C. Tellis, Amar Sunil Lobo, A. Motagi","doi":"10.51248/.v43i02.2464","DOIUrl":"https://doi.org/10.51248/.v43i02.2464","url":null,"abstract":"Introduction and Aim: This is a retrospective study to analyse the pathogens causing bacteraemia in COVID-19 patients and its correlation with inflammatory biomarkers such as procalcitonin (PCT), C-reactive protein (CRP), total WBC counts (TC) and D-dimer. The objectives of this study were to evaluate the blood culture positivity rate, to identify the pathogens causing bacteraemia, to analyse their antibiogram and to assess the significance of inflammatory markers along with patient outcomes.\u0000 \u0000Materials and Methods: This study included 165 COVID-19-positive patients admitted to the Intensive care unit (ICU) of a tertiary care hospital from June to September 2020. Blood culture, identification and antibiotic sensitivity testing (AST) were done using automated systems. Values of D-dimer, CRP, PCT and TC were obtained using immunoturbidimetric assay, chemiluminescent immunoassay, immunochromatographic testing and automated haematology analyser respectively.\u0000 \u0000Results: Among 143 blood culture samples obtained from 122 COVID-19 positive patients, 80 flagged positive. Out of the 80 isolates obtained, 53 (66.25%), 17 (21.25%) and 10 (12.5%) were gram-positive bacteria, gram-negative bacteria and candida respectively. The blood culture positivity rate was 25.4%. Acinetobacter spp. and K. pneumoniae showed high levels of antibiotic resistance. Among 16 patients with elevated PCT, 15 (93.7%) patients showed positive blood cultures. CRP of >5 mg/L and deranged total WBC counts were seen among all blood culture-positive patients. In 100 patients with elevated D-dimer, 54% (54/100) patients showed positive blood culture.\u0000 \u0000Conclusion: From this study, we conclude that early identification of pathogens and initiation of appropriate antimicrobial therapy is crucial for managing sepsis associated with COVID-19 infection. PCT, CRP, TC and D-dimer can help as biomarkers in the management of COVID-19 patients with secondary bloodstream infections (BSI).","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49388820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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