Shaimaa Noori Mahal, Ahmed Mohammed Turki, Elham Hazeim Abdulkareem
Introduction and Aim: Most cases of periodontitis are associated with microorganisms. The Gram positive bacteria Staphylococcus aureus is considered as one of the important organisms associated with periodontal infections. This study investigated the effect of silver nanoparticles as well as the antiseptic agent chlorhexidine on multi-drug resistant S. aureus isolated from periodontal infections. Materials and Methods: In this study, with help from dentists, 266 clinical samples were collected from dental patients who had periodontal infection. S. aureus isolated from samples was tested for their antibiotic susceptibility profiles. Silver nanoparticles and chlorhexidine were evaluated for their antibacterial activity against these S. aureus isolates. Results: S. aureus strains isolated from periodontal infection patients in this study were found to be multidrug resistant. AgNPs obtained using E. coli showed high inhibition of S. aureus growth when used in different concentrations (5, 10, 15, 20, 25mM). Chlorhexidine also exhibited antibacterial activity against S. aureus. Combination of AgNPs with penicillin and ciprofloxacin had an increasing significant effect on the sensitivity of S. aureus. Similarly, chlorhexidine in combination with penicillin and ciprofloxacin also showed an inhibitory effect on the growth of S. aureus. Conclusion: AgNPs and chlorhexidine combined with antibiotics used in treatment of S. aureus isolated from periodontal disease showed a good antibacterial effect which suggests its use as an antibacterial agent against periodontitis associated bacteria.
{"title":"Effects of silver nanoparticles on multiple drug-resistant strains of Staphylococcus aureus from periodontal infection: An alternative approach for antimicrobial therapy","authors":"Shaimaa Noori Mahal, Ahmed Mohammed Turki, Elham Hazeim Abdulkareem","doi":"10.51248/.v43i3.2822","DOIUrl":"https://doi.org/10.51248/.v43i3.2822","url":null,"abstract":"Introduction and Aim: Most cases of periodontitis are associated with microorganisms. The Gram positive bacteria Staphylococcus aureus is considered as one of the important organisms associated with periodontal infections. This study investigated the effect of silver nanoparticles as well as the antiseptic agent chlorhexidine on multi-drug resistant S. aureus isolated from periodontal infections.\u0000 \u0000Materials and Methods: In this study, with help from dentists, 266 clinical samples were collected from dental patients who had periodontal infection. S. aureus isolated from samples was tested for their antibiotic susceptibility profiles. Silver nanoparticles and chlorhexidine were evaluated for their antibacterial activity against these S. aureus isolates.\u0000 \u0000Results: S. aureus strains isolated from periodontal infection patients in this study were found to be multidrug resistant. AgNPs obtained using E. coli showed high inhibition of S. aureus growth when used in different concentrations (5, 10, 15, 20, 25mM). Chlorhexidine also exhibited antibacterial activity against S. aureus. Combination of AgNPs with penicillin and ciprofloxacin had an increasing significant effect on the sensitivity of S. aureus. Similarly, chlorhexidine in combination with penicillin and ciprofloxacin also showed an inhibitory effect on the growth of S. aureus.\u0000 \u0000Conclusion: AgNPs and chlorhexidine combined with antibiotics used in treatment of S. aureus isolated from periodontal disease showed a good antibacterial effect which suggests its use as an antibacterial agent against periodontitis associated bacteria.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43672611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sadiruldeen Sami Abed, Farqad Bader Hamdan, Qasim Sharhan Al-Mayah, Mahir Mohammed Hussein
Introduction and Aim: The miR-146a is a short non-coding RNA molecule that has both therapeutic and biomarker potential. Abnormal miR-146a expression has been linked to several disorders. The target of the study is to investigate the possible link between miRNA-146a expression and the deterioration of cognitive function in Alzheimer's patients. Materials and Methods: The research comprised 40 individuals from Iraq, spanning both genders and ranging in age from 60 to 85 years. They were segregated into two distinct groups. The first group included 40 subjects (age of 75±6.6 years) who displayed no signs of cognitive or functional impairment. The second group consisted of 40 patients diagnosed with Alzheimer's disease (with an average age of 74.98±5.03 years), as per the DSM-5 criteria. To assess the mir-146 gene expression, quantitative polymerase chain reaction (qPCR) was employed. Results: The median expression of miR-146a in Alzheimer's disease was 0.97-fold greater than in control, with no statistically significant difference. There were no significant differences in the means between Alzheimer's females and Alzheimer's males. Female control subjects had considerably higher mean ADAS-cog scores than male control subjects. In Alzheimer's disease patients, age was observed to be strongly associated with ADAS-Cog scores, showing that age plays a role in disease development. Conclusion: Serum miR-146a is not related to the development of Alzheimer disease and does not affect disease progression
{"title":"Do miR-146a plasma levels alter in Alzheimer’s patients and impact their cognitive functions?","authors":"Sadiruldeen Sami Abed, Farqad Bader Hamdan, Qasim Sharhan Al-Mayah, Mahir Mohammed Hussein","doi":"10.51248/.v43i3.2817","DOIUrl":"https://doi.org/10.51248/.v43i3.2817","url":null,"abstract":"Introduction and Aim: The miR-146a is a short non-coding RNA molecule that has both therapeutic and biomarker potential. Abnormal miR-146a expression has been linked to several disorders. The target of the study is to investigate the possible link between miRNA-146a expression and the deterioration of cognitive function in Alzheimer's patients.\u0000 \u0000Materials and Methods: The research comprised 40 individuals from Iraq, spanning both genders and ranging in age from 60 to 85 years. They were segregated into two distinct groups. The first group included 40 subjects (age of 75±6.6 years) who displayed no signs of cognitive or functional impairment. The second group consisted of 40 patients diagnosed with Alzheimer's disease (with an average age of 74.98±5.03 years), as per the DSM-5 criteria. To assess the mir-146 gene expression, quantitative polymerase chain reaction (qPCR) was employed.\u0000 \u0000Results: The median expression of miR-146a in Alzheimer's disease was 0.97-fold greater than in control, with no statistically significant difference. There were no significant differences in the means between Alzheimer's females and Alzheimer's males. Female control subjects had considerably higher mean ADAS-cog scores than male control subjects. In Alzheimer's disease patients, age was observed to be strongly associated with ADAS-Cog scores, showing that age plays a role in disease development.\u0000 \u0000Conclusion: Serum miR-146a is not related to the development of Alzheimer disease and does not affect disease progression","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42668445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. T, Kumar Vadlapudi, Prathap H.M., Poornima D.V., R. F., V. E., S. M., Anuradha Cm
Introduction and Aim: Anisomeles indica Kuntze (A. indica) is employed to treat a wide range of illnesses. The Present study was aimed at establishing preliminary phytochemical screening, UV-Visible spectrophotometric, FT-IR analysis, and evaluation of biological activities of A. indica extracts. Materials and Methods: A. indica leaves were employed for the Soxhlet extraction. Furthermore, the crude extracts were utilized for phytochemical analysis and quantitative estimations of phenolics and flavonoids. UV-Vis spectrophotometric, and FT-IR analysis provided further evidence for the existence of bioactive constituents in A. indica extracts. The A. indica extracts were assessed for antioxidant potential by DPPH and metal chelation activity. Results: The findings illustrated that A. indica methanol extract was found to possess the highest yield. The preliminary phytochemical screening, UV-Vis spectrophotometric, and FT-IR fingerprint analysis provided evidence for the existence of significant bioactive constituents. A.indica methanol extract has significant total phenol, flavonoid content, and TAC (total antioxidant capacity) among all extracts. These characteristics are attributed to substantial antioxidant activity and metal-chelating activity. Conclusion: The findings of this study imply that A. indica extract possess antioxidant activity as evaluated by the potential DPPH radical scavenging and chelate metal ions. These characteristics are interconnected to the high flavonoid and phenol content, and distinctive secondary metabolites. The finding indicates that A.indica is abundant in active phytoconstituents, which also offer a vital source for effective therapeutic management.
{"title":"Evaluation of in vitro antioxidant potential of Anisomeles indica Kuntze and exploration of its bioactive phytoconstituents","authors":"M. T, Kumar Vadlapudi, Prathap H.M., Poornima D.V., R. F., V. E., S. M., Anuradha Cm","doi":"10.51248/.v43i3.2541","DOIUrl":"https://doi.org/10.51248/.v43i3.2541","url":null,"abstract":"Introduction and Aim: Anisomeles indica Kuntze (A. indica) is employed to treat a wide range of illnesses. The Present study was aimed at establishing preliminary phytochemical screening, UV-Visible spectrophotometric, FT-IR analysis, and evaluation of biological activities of A. indica extracts. \u0000 \u0000Materials and Methods: A. indica leaves were employed for the Soxhlet extraction. Furthermore, the crude extracts were utilized for phytochemical analysis and quantitative estimations of phenolics and flavonoids. UV-Vis spectrophotometric, and FT-IR analysis provided further evidence for the existence of bioactive constituents in A. indica extracts. The A. indica extracts were assessed for antioxidant potential by DPPH and metal chelation activity. \u0000 \u0000Results: The findings illustrated that A. indica methanol extract was found to possess the highest yield. The preliminary phytochemical screening, UV-Vis spectrophotometric, and FT-IR fingerprint analysis provided evidence for the existence of significant bioactive constituents. A.indica methanol extract has significant total phenol, flavonoid content, and TAC (total antioxidant capacity) among all extracts. These characteristics are attributed to substantial antioxidant activity and metal-chelating activity. \u0000 \u0000Conclusion: The findings of this study imply that A. indica extract possess antioxidant activity as evaluated by the potential DPPH radical scavenging and chelate metal ions. These characteristics are interconnected to the high flavonoid and phenol content, and distinctive secondary metabolites. The finding indicates that A.indica is abundant in active phytoconstituents, which also offer a vital source for effective therapeutic management.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47313786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Presence of virulence factors may cause increased persistence of Enterococci in the healthcare environment, increase ability to colonise inpatients and thereby result in the transmission of infection. The present study was performed to detect the presence of virulence traits among the clinical strains of Enterococci and to determine its association between virulence factors and susceptibility to various antibiotics. Materials and Methods: Clinical isolates of Enterococci were identified to species level by conventional method and Vitek 2 automated method and subjected to antibiotic susceptibility testing using disk diffusion and Minimum Inhibitory Concentration (MIC) method. Presence of hemolysin, gelatinase and biofilm was detected by phenotypic method. Results: Out of 708 isolates from urine 39 (5.51%) Enterococcus faecalis, and 3 isolates each of Enterococcus faecium (0.42 %) and Enterococcus durans (0.42 %) were biofilm producers. Beta hemolysin production was detected in 342 (48.30%) E. faecalis obtained from urine and 9/48 (18.75 %) from pus. Out of the isolates studied, 9/774 (1.16 %) isolates were found to be positive for beta-hemolysin production, gelatinase and biofilm production. All the 9 (100.00 %) isolates were resistant to penicillin, high level gentamicin, ciprofloxacin and levofloxacin. Conclusion: Virulence factors described in Enterococci enable these organisms to colonise patient tissue, increase resistance to antimicrobial agents and aggravate infection outcome.
{"title":"A study on virulence determinants and antimicrobial resistance among Enterococci isolates","authors":"Reena Rajan, K. Dhandapani","doi":"10.51248/.v43i3.1160","DOIUrl":"https://doi.org/10.51248/.v43i3.1160","url":null,"abstract":"Introduction and Aim: Presence of virulence factors may cause increased persistence of Enterococci in the healthcare environment, increase ability to colonise inpatients and thereby result in the transmission of infection. The present study was performed to detect the presence of virulence traits among the clinical strains of Enterococci and to determine its association between virulence factors and susceptibility to various antibiotics.\u0000 \u0000Materials and Methods: Clinical isolates of Enterococci were identified to species level by conventional method and Vitek 2 automated method and subjected to antibiotic susceptibility testing using disk diffusion and Minimum Inhibitory Concentration (MIC) method. Presence of hemolysin, gelatinase and biofilm was detected by phenotypic method.\u0000 \u0000Results: Out of 708 isolates from urine 39 (5.51%) Enterococcus faecalis, and 3 isolates each of Enterococcus faecium (0.42 %) and Enterococcus durans (0.42 %) were biofilm producers. Beta hemolysin production was detected in 342 (48.30%) E. faecalis obtained from urine and 9/48 (18.75 %) from pus. Out of the isolates studied, 9/774 (1.16 %) isolates were found to be positive for beta-hemolysin production, gelatinase and biofilm production. All the 9 (100.00 %) isolates were resistant to penicillin, high level gentamicin, ciprofloxacin and levofloxacin.\u0000 \u0000Conclusion: Virulence factors described in Enterococci enable these organisms to colonise patient tissue, increase resistance to antimicrobial agents and aggravate infection outcome.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48602113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shilpa Dutta, Akash More, Priti Karadbhajne, D. Shrivastava
Platelet-rich plasma therapy, also truncated as PRP, has been remarkably utilized for quite long in the domains of cardiovascular surgery, orthopaedic treatments, sports medicines etc. Being in the limelight for its remarkable wound healing and cell proliferating activities, it has caught the interest of researchers in the field of reproductive sciences. The chief domain of research has revolved around thin endometrial lining, diminished ovarian content, recurrent implantation failure ailments etc. This article has focused on reviewing existing articles till now on the aetiology of PRP along with methodologies and results in reproductive aberrational conditions. Restrictive research is conducted from Scopus and Web of Science indexed journals, PubMed focusing on the deployment of PRP in infertility science and categorized the result under 3 genres: PRP in refractory attenuated endometrium, PRP in female gonadal abnormalities and PRP in Asherman’s syndrome. Most literature has shown promising results in improvement of thin endometrium, along with increase in AMH (Anti-Mullerian Hormone) level along with decrement of FSH (Follicle Stimulating Hormone) level, thereby improving ovarian reserve. It also showed reduced adhesion in the uterus after hysteroscopy, along with improvement in the menstrual cycle in case of Asherman’s syndrome, thereby increasing chances of clinical pregnancy. However, lack of standardized protocol is a major con in variation of results. Thus, until conclusive RCTs with larger sample size are conducted, PRP should be restricted to the experimental stage for now.
富含血小板的血浆疗法，也称为PRP，长期以来一直在心血管外科、整形外科、运动医学等领域得到显著应用。由于其显著的伤口愈合和细胞增殖活性而备受关注，它引起了生殖科学领域研究人员的兴趣。主要研究领域围绕着子宫内膜薄、卵巢内容物减少、复发性植入失败疾病等。本文重点回顾了迄今为止关于PRP病因的现有文章，以及生殖异常条件下的方法和结果。Scopus和Web of Science索引期刊PubMed进行了限制性研究，重点关注PRP在不孕科学中的应用，并将结果分为三类：难治性减薄子宫内膜中的PRP、女性性腺异常中的PRP-和Asherman综合征中的PRP。大多数文献显示，在改善薄子宫内膜、提高AMH（抗苗勒管激素）水平和降低FSH（卵泡刺激激素）水平方面取得了有希望的结果，从而改善卵巢储备。宫腔镜检查后，子宫粘连减少，Asherman综合征患者月经周期改善，从而增加了临床妊娠的机会。然而，缺乏标准化的方案是导致结果变化的主要因素。因此，在进行更大样本量的决定性随机对照试验之前，PRP目前应限制在实验阶段。
{"title":"Contribution of platelet-rich plasma therapy in reproductive health science: A narrative review","authors":"Shilpa Dutta, Akash More, Priti Karadbhajne, D. Shrivastava","doi":"10.51248/.v43i3.2678","DOIUrl":"https://doi.org/10.51248/.v43i3.2678","url":null,"abstract":"Platelet-rich plasma therapy, also truncated as PRP, has been remarkably utilized for quite long in the domains of cardiovascular surgery, orthopaedic treatments, sports medicines etc. Being in the limelight for its remarkable wound healing and cell proliferating activities, it has caught the interest of researchers in the field of reproductive sciences. The chief domain of research has revolved around thin endometrial lining, diminished ovarian content, recurrent implantation failure ailments etc. This article has focused on reviewing existing articles till now on the aetiology of PRP along with methodologies and results in reproductive aberrational conditions. Restrictive research is conducted from Scopus and Web of Science indexed journals, PubMed focusing on the deployment of PRP in infertility science and categorized the result under 3 genres: PRP in refractory attenuated endometrium, PRP in female gonadal abnormalities and PRP in Asherman’s syndrome. Most literature has shown promising results in improvement of thin endometrium, along with increase in AMH (Anti-Mullerian Hormone) level along with decrement of FSH (Follicle Stimulating Hormone) level, thereby improving ovarian reserve. It also showed reduced adhesion in the uterus after hysteroscopy, along with improvement in the menstrual cycle in case of Asherman’s syndrome, thereby increasing chances of clinical pregnancy. However, lack of standardized protocol is a major con in variation of results. Thus, until conclusive RCTs with larger sample size are conducted, PRP should be restricted to the experimental stage for now.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45501343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hardi Kapadia, Divya Vora, Dinesh S. Manjegowda, A. Nair, Sameer Sharma, Susha Dinesh
Introduction and Aim: Multiple sclerosis (MS) is a chronic neurodegenerative disease affecting around 2.8 million people worldwide. MS pathophysiology is not yet explained up to the mark, which is the cause of difficulty and complexity in treating the illness. Most present-day scenarios are engrossed in inhibiting central nervous system (CNS) inflammation. However, this is not enough, hence the present study aims at finding best neuroprotective treatment without adverse effects. Materials and Methods: In silico attempt to validate the phytocompounds from Nigella sativa and showcase their use for targeting the neuroprotective mechanism involved in management of MS by finding the key potential genes which were derived from mRNA datasets of previous research. Various bioinformatics tools and software such as GEO, String, ShinyGO, PyRx were used to carry out the current study. The leading steps involve retrieval of targets from mRNA datasets, molecular docking of phytocompounds with the targets and pharmacological analysis. Results: These phytocompounds from seeds of N. sativa showed promising results as therapeutic agents against target genes RPL27, RPS14 and FAU for management of MS during current in silico study, but any treatment prior its clinical practice should validate with large robust data, which lies as the future prospective here. Conclusion: In summary notable progress in management of MS with better understanding of pathology has been made and many disease modifying therapies (DMT) are made available but the question of safety and efficacy is still challenging due to adverse effects associated with these therapies. Hence properties of N. sativa must be explored as a therapeutic agent that can reduce the neuronal degeneration.
{"title":"Integrating network pharmacology and molecular docking for the identification of key genes and therapeutic targets of Nigella sativa in multiple sclerosis treatment","authors":"Hardi Kapadia, Divya Vora, Dinesh S. Manjegowda, A. Nair, Sameer Sharma, Susha Dinesh","doi":"10.51248/.v43i3.2867","DOIUrl":"https://doi.org/10.51248/.v43i3.2867","url":null,"abstract":"Introduction and Aim: Multiple sclerosis (MS) is a chronic neurodegenerative disease affecting around 2.8 million people worldwide. MS pathophysiology is not yet explained up to the mark, which is the cause of difficulty and complexity in treating the illness. Most present-day scenarios are engrossed in inhibiting central nervous system (CNS) inflammation. However, this is not enough, hence the present study aims at finding best neuroprotective treatment without adverse effects.\u0000 \u0000Materials and Methods: In silico attempt to validate the phytocompounds from Nigella sativa and showcase their use for targeting the neuroprotective mechanism involved in management of MS by finding the key potential genes which were derived from mRNA datasets of previous research. Various bioinformatics tools and software such as GEO, String, ShinyGO, PyRx were used to carry out the current study. The leading steps involve retrieval of targets from mRNA datasets, molecular docking of phytocompounds with the targets and pharmacological analysis.\u0000 \u0000Results: These phytocompounds from seeds of N. sativa showed promising results as therapeutic agents against target genes RPL27, RPS14 and FAU for management of MS during current in silico study, but any treatment prior its clinical practice should validate with large robust data, which lies as the future prospective here.\u0000 \u0000Conclusion: In summary notable progress in management of MS with better understanding of pathology has been made and many disease modifying therapies (DMT) are made available but the question of safety and efficacy is still challenging due to adverse effects associated with these therapies. Hence properties of N. sativa must be explored as a therapeutic agent that can reduce the neuronal degeneration.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42991282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Abdyldaev, Banur Uzakbaeva, B. Bebezov, Iana Karabaeva, Zhanna Abdyldaeva, T. Tagaev
Introduction and Aim: Breast infections are an increasing problem for women who are breastfeeding, with symptoms ranging from mastitis to the development of abscesses. The aim of this study is to develop a minimally invasive method of interventional continuous vacuum drainage for the treatment of lactational purulent mastitis. Materials and Methods: All 53 patients were comparable based on the duration of the disease prior to surgery and the timing of the emergence of mastitis after delivery, as well as the location and prevalence of the inflammatory process in the mammary gland. Results: During the single-catheter method of vacuum drainage for the treatment of lactational purulent mastitis, lactation of the patient's affected breast was good in 2 (4.2%) patients and satisfactory in 46 (95.8%) patients. 38 (792%) women had good lactation, whereas 10 (21.8%) women had adequate lactation. After using the single-catheter method of vacuum drainage to treat lactational purulent mastitis, good lactation was seen in 40 (83.3%) instances, and it was satisfactory in 8 (16.7%) of patients. Conclusion: A single-catheter interventional continuous vacuum drainage under ultrasound control was developed to treat lactational purulent mastitis without antibacterial therapy in 5–7 days, preserving lactation and achieving good aesthetic results.
{"title":"Single-catheter minimally invasive method of interventional continuous vacuum drainage for the treatment of lactational purulent mastitis","authors":"D. Abdyldaev, Banur Uzakbaeva, B. Bebezov, Iana Karabaeva, Zhanna Abdyldaeva, T. Tagaev","doi":"10.51248/.v43i3.2883","DOIUrl":"https://doi.org/10.51248/.v43i3.2883","url":null,"abstract":"Introduction and Aim: Breast infections are an increasing problem for women who are breastfeeding, with symptoms ranging from mastitis to the development of abscesses. The aim of this study is to develop a minimally invasive method of interventional continuous vacuum drainage for the treatment of lactational purulent mastitis.\u0000 \u0000Materials and Methods: All 53 patients were comparable based on the duration of the disease prior to surgery and the timing of the emergence of mastitis after delivery, as well as the location and prevalence of the inflammatory process in the mammary gland.\u0000 \u0000Results: During the single-catheter method of vacuum drainage for the treatment of lactational purulent mastitis, lactation of the patient's affected breast was good in 2 (4.2%) patients and satisfactory in 46 (95.8%) patients. 38 (792%) women had good lactation, whereas 10 (21.8%) women had adequate lactation. After using the single-catheter method of vacuum drainage to treat lactational purulent mastitis, good lactation was seen in 40 (83.3%) instances, and it was satisfactory in 8 (16.7%) of patients.\u0000 \u0000Conclusion: A single-catheter interventional continuous vacuum drainage under ultrasound control was developed to treat lactational purulent mastitis without antibacterial therapy in 5–7 days, preserving lactation and achieving good aesthetic results.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49362959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Praveena Ravichandran, K. Bhat, M. Hosapatna, P. L. C.
Introduction and Aim: The Circle of Willis (CoW) is an arterial hexagon in the interpeduncular fossa formed by the basilar and internal carotid arteries. It permits anastomotic circulation between that two-arterial system. Variations in the pattern and the calibre of the arteries that make up the CoW were common. Few noted variations include hypoplasia or aplasia of either one or both posterior or anterior communicating arteries, aplasia, or fenestrations of the anterior communicating artery. Considering the surgical importance of this arterial circle, as it allows equalization of blood flow between the two sides of the brain, this observational study was done to identify the variations in the formation pattern and the gross morphometry of CoW. Materials and Methods: Thirty radiological images of patients undergoing neurovascular imaging for various reasons in the Department of Radiology and patients were obtained for the study. Results: Among the 30 studied CT and MR angiographic images, 21 (73.3%) of the circles exhibit the usual pattern of CoW formation, and the remaining 9 (27.7%) showed variations. Among the 30 radiological images, 25 (83.33%) were complete circles, 4 (13.33%) were incomplete in the posterior part and 1 (3.33%) was incomplete in the anterior part of the CoW. Conclusion: Understanding the typical formation of CoW and its variations is essential in the surgical correction of a few intracranial emergencies like hemorrhage, infarction, aneurysms, and other neurovascular surgeries of the brain.
{"title":"Radiological observations of the branches of circle of willis for surgical perception","authors":"Praveena Ravichandran, K. Bhat, M. Hosapatna, P. L. C.","doi":"10.51248/.v43i3.2557","DOIUrl":"https://doi.org/10.51248/.v43i3.2557","url":null,"abstract":"Introduction and Aim: The Circle of Willis (CoW) is an arterial hexagon in the interpeduncular fossa formed by the basilar and internal carotid arteries. It permits anastomotic circulation between that two-arterial system. Variations in the pattern and the calibre of the arteries that make up the CoW were common. Few noted variations include hypoplasia or aplasia of either one or both posterior or anterior communicating arteries, aplasia, or fenestrations of the anterior communicating artery. Considering the surgical importance of this arterial circle, as it allows equalization of blood flow between the two sides of the brain, this observational study was done to identify the variations in the formation pattern and the gross morphometry of CoW.\u0000 \u0000Materials and Methods: Thirty radiological images of patients undergoing neurovascular imaging for various reasons in the Department of Radiology and patients were obtained for the study.\u0000 \u0000Results: Among the 30 studied CT and MR angiographic images, 21 (73.3%) of the circles exhibit the usual pattern of CoW formation, and the remaining 9 (27.7%) showed variations. Among the 30 radiological images, 25 (83.33%) were complete circles, 4 (13.33%) were incomplete in the posterior part and 1 (3.33%) was incomplete in the anterior part of the CoW.\u0000 \u0000Conclusion: Understanding the typical formation of CoW and its variations is essential in the surgical correction of a few intracranial emergencies like hemorrhage, infarction, aneurysms, and other neurovascular surgeries of the brain.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48129924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nishanthi Raja, A. Ganesan, Swathi K. V., K. A, Krithika Chandrasekar Lakshmi
Introduction and Aim: Prebiotics and probiotics are essential for improving overall health. Pre- and probiotics are becoming more popular in oral care products. Also, in recent years, oral medicine has been provided to them. The study's objective is to assess dental postgraduates' attitudes, knowledge, and awareness of prebiotics and probiotics for oral lesions. Materials and Methods: A set of 20 questions related to the definition, mechanism of action, safety consideration, health benefits, and sources, and four questions to assess their attitude and practice about probiotics and prebiotics in oral lesions addressing a total number of 183 dental postgraduates were selected. Google form questionnaires were shared, and responses were received. The obtained data were statistically analyzed. Results: Of the 183 dental postgraduates 91.3% of the dental postgraduates were aware of the use of probiotics and prebiotics in oral lesions while 92.2% were willing to prescribe them to their patients and consider them to be safe and used as an adjuvant in the management of oral lesions. Results showed limited knowledge and awareness of probiotics and prebiotics in the management of oral lesions among dental postgraduates. Conclusion: The result indicated a lack of understanding of prebiotics and probiotics in the treatment of oral lesions. It is possible to increase knowledge of prebiotics and probiotics in oral lesions through seminars, current lectures, and including the subject in the curricula of aspiring professionals, among other methods.
{"title":"Awareness of prebiotics and probiotics in oral lesions among dental postgraduates -A questionnaire-based study","authors":"Nishanthi Raja, A. Ganesan, Swathi K. V., K. A, Krithika Chandrasekar Lakshmi","doi":"10.51248/.v43i3.2716","DOIUrl":"https://doi.org/10.51248/.v43i3.2716","url":null,"abstract":"Introduction and Aim: Prebiotics and probiotics are essential for improving overall health. Pre- and probiotics are becoming more popular in oral care products. Also, in recent years, oral medicine has been provided to them. The study's objective is to assess dental postgraduates' attitudes, knowledge, and awareness of prebiotics and probiotics for oral lesions.\u0000 \u0000Materials and Methods: A set of 20 questions related to the definition, mechanism of action, safety consideration, health benefits, and sources, and four questions to assess their attitude and practice about probiotics and prebiotics in oral lesions addressing a total number of 183 dental postgraduates were selected. Google form questionnaires were shared, and responses were received. The obtained data were statistically analyzed.\u0000 \u0000Results: Of the 183 dental postgraduates 91.3% of the dental postgraduates were aware of the use of probiotics and prebiotics in oral lesions while 92.2% were willing to prescribe them to their patients and consider them to be safe and used as an adjuvant in the management of oral lesions. Results showed limited knowledge and awareness of probiotics and prebiotics in the management of oral lesions among dental postgraduates.\u0000 \u0000Conclusion: The result indicated a lack of understanding of prebiotics and probiotics in the treatment of oral lesions. It is possible to increase knowledge of prebiotics and probiotics in oral lesions through seminars, current lectures, and including the subject in the curricula of aspiring professionals, among other methods.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42525722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Janani Maheshwari V. Vyas, S. Kumar S., Leena Dennis Joseph, K. P, S. R
Introduction and Aim: Various herbs have been prescribed as a cure for renal disorders by early literature. Nephroprotective herbs are protective against nephrotoxicity. Tinospora cordifolia is known for its role in treating diabetes and disorders of the kidney and metabolism. However, studying its protective effect on drug induced nephrotoxicity at different time periods is wanting. The aim is to study the nephroprotective effect of Tinospora cordifolia on drug induced nephrotoxic changes upon co-administration of the herb with nephrotoxicity induction by the drug. Materials and Methods: Ethanolic extract of the stem of Tinospora cordifolia was prepared and evaluated for phytochemical constituents. Gentamicin induced nephrotoxicity model in adult, male, Wistar rats was utilised for the study. Gentamicin and the extract of Tinospora cordifolia were co-administered for 8 days. In the kidney, levels of superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione and lipid peroxidation were measured. Assessment of the renal tissue was carried out using histopathological severity grading. Results: Phytochemicals like glycosides, flavonoids, saponins, steroids, quinone and coumarin were present in the stem of Tinospora cordifolia. Analysing the levels of oxidative stress and antioxidants in the kidney revealed the rise in the level of reduced glutathione (GSH). This correlated with the regenerative changes brought about by the herbal extract on the acute tubular necrosis in the renal tissue induced due to gentamicin nephrotoxicity, at the histological levels in terms of the reduction of enlargement and regeneration of the tubular epithelium. Conclusion: Tinospora cordifolia extract constituted flavonoids which are known antioxidants. Co-administration of Tinospora cordifolia stem extract is protective in the nephrotoxic condition induced by drugs, hence, shall benefit the kidney when taken along while consuming potentially nephrotoxic drugs.
{"title":"Co-administration with Tinospora cordifolia attenuates drug induced nephrotoxicity – A histological and biochemical assessment","authors":"Janani Maheshwari V. Vyas, S. Kumar S., Leena Dennis Joseph, K. P, S. R","doi":"10.51248/.v43i3.1698","DOIUrl":"https://doi.org/10.51248/.v43i3.1698","url":null,"abstract":"Introduction and Aim: Various herbs have been prescribed as a cure for renal disorders by early literature. Nephroprotective herbs are protective against nephrotoxicity. Tinospora cordifolia is known for its role in treating diabetes and disorders of the kidney and metabolism. However, studying its protective effect on drug induced nephrotoxicity at different time periods is wanting. The aim is to study the nephroprotective effect of Tinospora cordifolia on drug induced nephrotoxic changes upon co-administration of the herb with nephrotoxicity induction by the drug.\u0000 \u0000Materials and Methods: Ethanolic extract of the stem of Tinospora cordifolia was prepared and evaluated for phytochemical constituents. Gentamicin induced nephrotoxicity model in adult, male, Wistar rats was utilised for the study. Gentamicin and the extract of Tinospora cordifolia were co-administered for 8 days. In the kidney, levels of superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione and lipid peroxidation were measured. Assessment of the renal tissue was carried out using histopathological severity grading.\u0000 \u0000Results: Phytochemicals like glycosides, flavonoids, saponins, steroids, quinone and coumarin were present in the stem of Tinospora cordifolia. Analysing the levels of oxidative stress and antioxidants in the kidney revealed the rise in the level of reduced glutathione (GSH). This correlated with the regenerative changes brought about by the herbal extract on the acute tubular necrosis in the renal tissue induced due to gentamicin nephrotoxicity, at the histological levels in terms of the reduction of enlargement and regeneration of the tubular epithelium.\u0000 \u0000Conclusion: Tinospora cordifolia extract constituted flavonoids which are known antioxidants. Co-administration of Tinospora cordifolia stem extract is protective in the nephrotoxic condition induced by drugs, hence, shall benefit the kidney when taken along while consuming potentially nephrotoxic drugs.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42560329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：