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Crossmodal Associations Between Olfaction and Vision: Color and Shape Visualizations of Odors 嗅觉和视觉之间的跨模态关联:气味的颜色和形状可视化
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2018-03-27 DOI: 10.1007/s12078-018-9245-y
Kathrin Kaeppler

In the present study, we assessed crossmodal associations between odors and both color and shape, with particular interest in the principles beneath these mappings. We hypothesized that visual associations of odors would primarily reflect observable features of a smelling object and thus vary with different source assumptions of the very same smell.

We asked 30 participants to visualize their odor associations on a drawing tablet, freely deciding on color and shape. Additionally, subjects provided ratings on perceptual and shape-related dimensions as well as a verbal label for each sample.

With respect to color selection, the results confirmed a source-based mapping approach: odors rated as familiar were associated with very particular colors that typically resembled the appearance of their source. For less familiar odors, color selection was rather inconsistent but still then went along with assumed odor objects. Shape ratings changed with odor identifications as well, but considerably less than for color associations. Shape ratings and shape drawings produced very different results. While shape ratings were unlikely rooted in the mental imagery of a shape, drawings frequently displayed concrete objects that depended on odor label.

Results confirm the existence of stable odor–vision correspondences and suggest that language plays a major part in mediating these mappings. The frequently assumed hedonic foundation of crossmodal matchings could not be confirmed for this stimuli set.

Odor sensations may trigger odor naming spontaneously. Assumptions about an odor’s identity, as well as the multisensory knowledge we have acquired on it, affect the visual associations of an odor.

在本研究中,我们评估了气味与颜色和形状之间的跨模态关联,并对这些映射下的原理特别感兴趣。我们假设气味的视觉关联将主要反映闻到物体的可观察特征,因此对相同气味的不同来源假设会有所不同。我们要求30名参与者在绘图板上想象他们的气味联想,自由决定颜色和形状。此外,受试者还提供了感知和形状相关维度的评分,以及每个样本的口头标签。在颜色选择方面,结果证实了一种基于源的映射方法:被评为熟悉的气味与非常特殊的颜色相关联,这些颜色通常与它们的源的外观相似。对于不太熟悉的气味,颜色选择相当不一致,但仍然遵循假设的气味对象。形状评级也随着气味的识别而改变,但比颜色关联要小得多。形状评级和形状图产生了非常不同的结果。虽然形状评级不太可能植根于对形状的心理意象,但绘画中经常显示的具体物体依赖于气味标签。结果证实了稳定的气味-视觉对应关系的存在,并表明语言在调解这些映射中起着重要作用。通常假设的跨模态匹配的享乐基础不能被这个刺激集证实。气味感觉可以自发地触发气味命名。对气味身份的假设,以及我们所获得的多感官知识,都会影响气味的视觉联想。
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引用次数: 20
Familiarity and Retronasal Aroma Alter Food Perception 熟悉度和鼻后香气改变食物感知
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2018-02-13 DOI: 10.1007/s12078-018-9244-z
Naomi Gotow, Wolfgang Skrandies, Takefumi Kobayashi, Tatsu Kobayakawa

When participants eat foods, they direct their attention to the particular taste quality that is the target of evaluation and subsequently perceive the intensity of the attended taste quality. We defined the ease with which participants pay attention to a particular taste quality as the “noticeability” of that quality. In our previous study, Japanese participants evaluated noticeability and intensity of five fundamental taste qualities (sweetness, umami, saltiness, sourness, and bitterness) under open- and closed-nostril conditions, using a popular traditional Japanese confection, yokan. The correlation between noticeability and intensity of sweetness was significantly reduced when participants were tested with open nostrils. Therefore, we hypothesized that high familiarity with a food and its olfactory information is necessary to decrease the correlation between these two scales.

In order to verify this hypothesis, we asked Japanese and German participants, who have different food cultures, to subjectively evaluate yokan, which is familiar to Japanese but unfamiliar to Germans. In a control condition, marshmallows were used which are familiar to all participants. Participants consumed each food under open- and closed-nostril conditions and evaluated the noticeability and intensity of the five fundamental taste qualities.

There were significant differences between the participants’ groups as the correlation between noticeability and intensity was reduced significantly only for sweetness of a familiar food under open-nostril condition.

These results support our hypothesis that high familiarity with a food and its olfactory information might be necessary to decrease the correlation between noticeability and intensity of a particular taste quality.

This finding suggests that perception of a food is influenced by its familiarity and retronasal aroma. These results suggest when a food is unfamiliar, the noticeability and intensity of a particular taste quality are not much altered by the retronasal aroma, but when a food is highly familiar, retronasal aroma serves to decouple noticeability and intensity of a given taste quality.

当参与者吃食物时,他们会将注意力集中在特定的味道质量上,这是评估的目标,随后会感知到被关注的味道质量的强度。我们将参与者对某种特定味道的注意程度定义为这种味道的“可注意性”。在我们之前的研究中,日本参与者在打开和关闭鼻孔的条件下评估了五种基本味觉品质(甜味、鲜味、咸味、酸味和苦味)的显着性和强度,使用的是一种流行的日本传统糖果——yokan。当参与者张开鼻孔进行测试时,甜度和甜度之间的相关性显著降低。因此,我们假设对食物及其嗅觉信息的高度熟悉对于降低这两个尺度之间的相关性是必要的。为了验证这一假设,我们让饮食文化不同的日本和德国参与者主观评价日本人熟悉但德国人不熟悉的yokan。在控制条件下,使用了所有参与者都熟悉的棉花糖。参与者在打开和关闭鼻孔的条件下食用每种食物,并评估五种基本味道品质的显著性和强度。受试者组之间存在显著差异,因为只有在打开鼻孔的情况下,熟悉食物的甜味之间的相关性和强度才显著降低。这些结果支持了我们的假设,即对食物及其嗅觉信息的高度熟悉可能是降低特定味道质量的可见度和强度之间的相关性所必需的。这一发现表明,对食物的感知受到其熟悉度和鼻后气味的影响。这些结果表明,当一种食物是不熟悉的,一种特定味道质量的显著性和强度不会被后鼻香气改变太多,但当一种食物是非常熟悉的,后鼻香气可以将一种特定味道质量的显著性和强度分离开来。
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引用次数: 9
Bibliometric Study on Functional Magnetic Resonance Imaging Literature (1995–2017) Concerning Chemosensory Perception 化学感觉知觉相关功能磁共振成像文献计量学研究(1995-2017
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2018-01-18 DOI: 10.1007/s12078-018-9243-0
Andy Wai Kan Yeung

This was the first bibliometric study to investigate the functional magnetic resonance (fMRI) literature on chemosensory perception to reveal who contributed to these publications, where they were published, what concepts were investigated, and their relative impact in terms of citations.

Web of Science was searched to identify relevant articles. Research areas, authors, contributing countries and territories, journals, and publication years were recorded. The full record and cited references of these articles were used for further bibliometric analyses and network visualization.

The search yielded 5200 unique authors who had contributed to 1660 articles. The authors in the co-authorship network generally had higher numbers of citations per article than those in the periphery did. The 1660 articles were published in 423 journals, and the distribution did not fulfill Bradford’s law. Articles with contributions from European countries and the USA appeared to have more citations on average relative to those from Asian countries.

The contributions came from a diverse spectrum of authors who had published in journals in multiple disciplines. Papers involving the insula, orbitofrontal cortex, and amygdala had accumulated high numbers of total citations. The hedonic aspect of chemosensory perception in particular had received many citations.

The fMRI literature on chemosensory perception provides substantial neurobiological findings from multiple authors, countries, and journals. These results offer a contemporary overview of the literature and facilitate researchers in identifying relevant papers for future references.

这是第一个调查功能性磁共振(fMRI)化学感觉感知文献的文献计量学研究,以揭示谁对这些出版物做出了贡献,它们在哪里发表,研究了哪些概念,以及它们在引用方面的相对影响。在Web of Science上搜索相关文章。记录了研究领域、作者、贡献国家和地区、期刊和出版年份。这些文章的完整记录和引用文献被用于进一步的文献计量分析和网络可视化。这项搜索产生了5200位独特的作者,他们贡献了1660篇文章。共同作者网络中的作者每篇文章的引用次数通常高于外围网络中的作者。1660篇文章发表在423种期刊上,分布不符合布拉德福德定律。来自欧洲国家和美国的文章似乎比来自亚洲国家的文章平均有更多的引用。这些贡献来自不同领域的作者,他们在多个学科的期刊上发表过文章。涉及脑岛、眶额皮质和杏仁核的论文累积了大量的总引用。特别是化学感觉知觉的享乐方面已经得到了许多引用。关于化学感觉知觉的fMRI文献提供了来自多个作者、国家和期刊的大量神经生物学发现。这些结果提供了文献的当代概述,并促进研究人员在确定相关论文,以供将来参考。
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引用次数: 27
Affective Personality Traits in Olfactory Dysfunction: the Role of Dysthymia and Arousal 嗅觉功能障碍中的情感性人格特征:心境恶劣和觉醒的作用
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2017-12-14 DOI: 10.1007/s12078-017-9242-6
Anne Schienle, Axel Wolf, Peter Valentin Tomazic, Rottraut Ille

Olfactory dysfunction can have a negative impact on emotional well-being. The aim of the present study was to examine associations between olfactory deficits and two affective personality characteristics (trait anxiety/trait depression).

A questionnaire study was conducted with a total of 116 participants (33 classified as anosmic, 40 as hyposmic, and 39 as normosmic). All participants gave self-reports on two facets of trait depression (dysthymia, euthymia) and trait anxiety (arousal, worrying). Due to the fact that in all three groups, trait depression and anxiety were substantially correlated, analyses of covariance were conducted.

After controlling for trait depression, anosmic and hyposmic patients showed lower trait arousal compared to normosmic controls (partial η 2?=?.05). After controlling for trait anxiety, patients scored higher on dysthymia (partial η 2?=?.06).

This study underlines the importance of statistically isolating specific associations between each of these affective personality characteristics and olfactory dysfunction.

The present findings suggest that olfactory dysfunction can have opposite effects on facets of trait depression and trait anxiety.

嗅觉功能障碍会对情绪健康产生负面影响。本研究的目的是研究嗅觉缺陷与两种情感人格特征(特质焦虑/特质抑郁)之间的关系。对116名参与者进行问卷调查研究(33名被分类为嗅觉缺失,40名被分类为嗅觉缺失,39名被分类为正常)。所有参与者都对特质抑郁(心境恶劣、心境愉悦)和特质焦虑(兴奋、担忧)的两个方面进行了自我报告。由于在所有三组中,特质性抑郁与焦虑均存在显著相关,因此进行协方差分析。在控制特质抑郁后,嗅觉缺失和低嗅觉缺失患者的特质唤醒比正常对照组低(部分η 2?=? 0.05)。在控制特质焦虑后,患者在心境恶劣方面得分更高(部分η 2?=?.06)。这项研究强调了统计分离这些情感人格特征和嗅觉功能障碍之间特定关联的重要性。目前的研究结果表明,嗅觉功能障碍可能对特质抑郁和特质焦虑有相反的影响。
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引用次数: 5
Development of Children’s Olfactory Abilities and Odor Awareness Is Not Predicted by Temperament: a Longitudinal Study 儿童嗅觉能力和气味意识的发展不受气质的影响:一项纵向研究
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2017-12-11 DOI: 10.1007/s12078-017-9240-8
Lenka Martinec Nováková, Jitka Fialová, Jan Havlíček

Temperament affects olfaction in cross-sectional studies. However, it is not clear whether it is linked to olfactory development. Here we examined the links between temperament and olfaction over a nearly 2-year period, expecting that children showing higher levels of negative affectivity would exhibit greater odor awareness across repeated testing. As a subsidiary aim, we investigated whether olfactory scores differ as a function of olfactory performance at a younger age.

The participants were 73 Czech children (mean age?=?5.8?years). Their olfactory abilities were assessed using the Sniffin’ Sticks, and odor awareness was evaluated with the Children’s Olfactory Behaviors in Everyday Life Questionnaire. These were reassessed 21?months later. Mothers provided information on the children’s temperament by completing the Short Form of the Children’s Behavior Questionnaire at the same interval.

Children’s temperament was not found to relate to olfactory development over a 21-month period. Odor identification and awareness, but not odor discrimination and threshold, differed as a function of scores achieved at an earlier age.

Odor identification and awareness predict themselves over a 21-month period. Links between temperament and olfaction may be only beginning to form at preschool age.

While this study suggests that temperament does not play a role in olfactory development, longitudinal studies over a broader timespan are needed to determine the robustness of this finding.

在横断面研究中,气质影响嗅觉。然而,尚不清楚它是否与嗅觉发育有关。在这里,我们在近2年的时间里研究了气质和嗅觉之间的联系,期望在重复测试中表现出更高水平的消极情感的孩子会表现出更强的气味意识。作为辅助目的,我们调查了嗅觉得分是否在年轻时作为嗅觉表现的函数而有所不同。参与者是73名捷克儿童(平均年龄5.8岁)。用嗅探棒评估儿童的嗅觉能力,用儿童日常生活嗅觉行为问卷评估儿童的嗅觉意识。这些都被重新评估过。个月后。母亲在同一时间间隔内通过填写《儿童行为问卷简表》提供有关儿童性情的信息。在21个月的时间里,儿童的气质与嗅觉发育没有关系。气味识别和意识,而不是气味辨别和阈值,作为早期得分的函数而不同。气味识别和意识可以在21个月的时间内预测自己。气质和嗅觉之间的联系可能在学龄前才开始形成。虽然这项研究表明气质在嗅觉发育中不起作用,但需要在更广泛的时间范围内进行纵向研究,以确定这一发现的稳健性。
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引用次数: 2
Exploring Facets of Flavor 探索风味的方方面面
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2017-12-11 DOI: 10.1007/s12078-017-9241-7
Jeannine F. Delwiche
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引用次数: 0
Effects of Ethanol on Flavor Perception in Alcoholic Beverages 乙醇对酒精饮料风味感知的影响
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2017-12-04 DOI: 10.1007/s12078-017-9238-2
Chelsea M. Ickes, Keith R. Cadwallader

In terms of both its physiological effects as well as its impact on flavor perception, ethanol is the defining component of alcoholic beverages. While ethanol’s effect on alcoholic beverage systems has been studied across a variety of disciplines, a comprehensive review of the literature is lacking. The aim of this paper is to review the current literature on ethanol’s effect on flavor perception.

This paper reviews the literature, exploring ethanol’s effects on the areas of physiochemical properties, instrumental measurements of flavor release, and sensory evaluation across a variety of alcoholic beverages including beer, wine, and distilled beverages and how changes in ethanol concentration can affect flavor perception.

Physicochemical properties such as the molecular level structure of water/ethanol matrices are significantly altered as a function of ethanol concentration. Of particular importance to flavor perception is the effect of ethanol on the release of aroma compounds into the headspace of the beverage. Both static and dynamic headspace systems have been studied. In general, increasing the concentration of ethanol decreases the volatile headspace in static systems, while it tends to increase the volatile headspace in dynamic systems. Most importantly, sensory studies have demonstrated that ethanol concentration significantly changes the flavor profile of beverages to the point where sensory panelists are able to identify and articulate these differences.

The current literature demonstrates that ethanol can have a huge impact of flavor release and sensory perception of alcoholic beverages. Additionally monitoring ethanol’s effects in systems as close to real life as possible (i.e., dynamic vs static evaluation) is important. Even with the extensive knowledge in the area, there are still many gaps in our understanding of ethanol’s effects on the flavor of alcoholic beverages.

Ethanol has been shown to have a tremendous influence on flavor release and subsequent chemosensory perception in alcoholic beverages. Changes in ethanol concentration can affect consumers’ perception of alcoholic beverages in terms of aroma, taste, and mouthfeel.

就其生理作用以及对风味感知的影响而言,乙醇是酒精饮料的决定性成分。虽然乙醇对酒精饮料系统的影响已经在各种学科中进行了研究,但缺乏对文献的全面回顾。本文的目的是回顾目前关于乙醇对风味感知影响的文献。本文回顾了相关文献,探讨了乙醇对各种酒精饮料(包括啤酒、葡萄酒和蒸馏饮料)的理化性质、风味释放的仪器测量和感官评价的影响,以及乙醇浓度的变化如何影响风味感知。物理化学性质,如水/乙醇基质的分子水平结构,作为乙醇浓度的函数显著改变。对风味感知特别重要的是乙醇对香气化合物释放到饮料顶部空间的影响。静态和动态顶空系统都进行了研究。一般来说,增加乙醇浓度会降低静态系统的挥发性顶空,而增加动态系统的挥发性顶空。最重要的是,感官研究表明,乙醇浓度显著改变了饮料的风味特征,以至于感官小组成员能够识别和阐明这些差异。目前的文献表明,乙醇可以对酒精饮料的风味释放和感官知觉产生巨大的影响。此外,监测系统中乙醇的影响,尽可能接近现实生活(即,动态与静态评估)是很重要的。即使在这一领域有了广泛的知识,我们对乙醇对酒精饮料风味的影响的理解仍有许多空白。乙醇已被证明对酒精饮料中的风味释放和随后的化学感官知觉有巨大的影响。乙醇浓度的变化会影响消费者对酒精饮料的香气、味道和口感的感知。
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引用次数: 40
Fast Olfactory Threshold Determination Using an Ascending Limits Procedure 快速嗅觉阈值测定使用上升限制程序
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2017-11-21 DOI: 10.1007/s12078-017-9239-1
Rik Sijben, Claudia Panzram, Rea Rodriguez-Raecke, Thomas Haarmeier, Jessica Freiherr

The Sniffin’ Sticks test battery is currently considered the best alternative for the measurement of olfactory threshold, discrimination and identification capabilities. These tests still suffer from limitations, however. Most noticeably, the olfactory threshold test is an intensive task which requires participants to smell a large number of olfactory stimuli. This proves especially problematic when measuring olfactory performance of elderly patients or screening research subjects, as sensory adaptation plays an important role in olfactory perception.

In the current study, we have determined that the cause of this limitation lies with the test’s single-staircase procedure (SSP). Consequentially, we have devised an alternative ascending limits procedure (ALP). We here compared data obtained using both procedures, following a within-subject design with 40 participants. Olfactory threshold scores as well as number of trials required to complete the two procedures were investigated.

The results show that the ALP provides reliable and correct olfactory threshold ratings, as the values showed a good correlation with those obtained using the SSP and mean values did not differ significantly. Task duration, however, did show a highly significant difference, completing the SSP required participants to complete over 40% more trials compared to the ALP.

The here presented methodological improvement can save time and, more importantly, reduce participants’ cognitive and sensory strain, which is not only more comfortable, but also limits the influence of adaptation, making any measured data more reliable.

Improving standard screening methods can directly enhance the reliability of any future study using this procedure.

嗅探棒测试电池目前被认为是测量嗅觉阈值、辨别和识别能力的最佳选择。然而,这些测试仍然存在局限性。最值得注意的是,嗅觉阈值测试是一项密集的任务,要求参与者闻到大量的嗅觉刺激。这在测量老年患者的嗅觉表现或筛选研究对象时尤其成问题,因为感觉适应在嗅觉感知中起着重要作用。在目前的研究中,我们已经确定这种限制的原因在于测试的单阶梯程序(SSP)。因此,我们设计了一个替代的上升极限程序(ALP)。我们在这里比较了使用这两种方法获得的数据,遵循40名参与者的受试者内设计。嗅觉阈值得分以及完成这两个程序所需的试验次数进行了调查。结果表明,ALP提供了可靠和正确的嗅觉阈值评级,因为这些值与使用SSP获得的值显示出良好的相关性,并且平均值没有显着差异。然而,任务持续时间确实显示出高度显著的差异,完成SSP需要参与者比ALP多完成40%以上的试验。本文提出的方法改进可以节省时间,更重要的是减少参与者的认知和感觉紧张,这不仅更舒适,而且限制了适应的影响,使任何测量数据更可靠。改进标准筛选方法可以直接提高使用该程序的任何未来研究的可靠性。
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引用次数: 5
Dysosmia-Associated Changes in Eating Behavior 与进食障碍相关的改变
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2017-10-13 DOI: 10.1007/s12078-017-9237-3
C. Manesse, C. Ferdenzi, M. Sabri, M. Bessy, C. Rouby, F. Faure, D. Bellil, S. Jomain, B. N. Landis, M. Hugentobler, M. Cuevas, T. Hummel, M. Bensafi

Olfaction is a highly emotionally charged sense and contributes to our quality of life, which olfactory impairment or dysosmia thus strongly impacts. The aim of the present study was to examine how olfactory deficits alter eating behavior, which is a pillar of health and well-being.

Patients with quantitative smell impairment and control participants were asked to perform a series of chemosensory tasks: odor identification and ratings of odor intensity, pleasantness, familiarity, irritation, and edibility. They also filled out a detailed food questionnaire.

Results showed significant decrease in olfactory function in smell-impaired patients. Although no significant consequences of dysosmia were found for most aspects of food preferences and culinary habits, the patients were less attracted than controls by novel foods and tended to experience less pleasure when eating. They also used significantly more condiments such as sugar, mayonnaise, or sour cream to make their dishes tasty.

Olfactory impairment has a clear effect on certain aspects of eating behavior.

These findings highlight the compensatory mechanisms that go along with dysosmia. This also reflects the patients’ attempts to restore part of the lost flavor and its hedonic component through non-olfactory cues.

嗅觉是一种高度情绪化的感觉,有助于我们的生活质量,嗅觉障碍或嗅觉障碍因此强烈影响。本研究的目的是研究嗅觉缺陷如何改变饮食行为,这是健康和幸福的支柱。定量嗅觉障碍患者和对照组参与者被要求执行一系列化学感官任务:气味识别和气味强度、愉悦性、熟悉性、刺激性和可食性的评级。他们还填写了一份详细的食物调查问卷。结果显示嗅觉受损患者的嗅觉功能明显下降。虽然在食物偏好和烹饪习惯的大多数方面没有发现嗅觉障碍的显著后果,但与对照组相比,患者对新食物的吸引力更小,而且在进食时往往体验到更少的快乐。他们还使用了更多的调味品,如糖、蛋黄酱或酸奶油,以使他们的菜肴美味。嗅觉障碍对饮食行为的某些方面有明显的影响。这些发现强调了伴随嗅觉障碍的代偿机制。这也反映了患者试图通过非嗅觉线索恢复部分失去的味道及其快乐成分。
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引用次数: 30
Comparative Characterization of Aroma Compounds in Merlot Wine by LiChrolut-EN-Based Aroma Extract Dilution Analysis and Odor Activity Value 以lichrolut - en为基础的香气提取液稀释分析及气味活性值对梅洛葡萄酒香气成分的比较表征
IF 1 4区 医学 Q4 Neuroscience Pub Date : 2017-10-12 DOI: 10.1007/s12078-017-9236-4
Pengtao Zhao, Yanping Qian, Fei He, Hua Li, Michael Qian

It has been challenging to select the best method to extract and analyze aroma-active compounds in wine due to high concentration of alcohols in the sample. The objectives of this study were to investigate the applicability of a LiChrolut-EN-based solid-phase extraction (SPE) for wine aroma extraction and to characterize the aroma-active compounds in Merlot wine.

Volatile compounds in a Merlot wine were extracted with Lichrolut-EN SPE or liquid-liquid extraction (LLE) followed by solvent-assisted flavor evaporation (SAFE). Both extracts were used for aroma extract dilution analysis (AEDA). Selected aroma-active compounds were further quantitated and their odor activity values (OAV) were calculated.

Both SPE and LLE extracts can be used for aroma extract dilution analysis. The odorants with high flavor dilution (FD ≥?64) values in Merlot wine were ethyl 2-methylpropanoate, ethyl butanoate, ethyl 2-methylbutanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, 3-methylbutyl acetate, 2/3-methyl-1-butanol, 2-phenylethanol, acetic acid, 2-methylpropanoic acid, butanoic acid, 3-methylbutanoic acid, hexanoic acid, β-damascenone, E-whiskeylactone, γ-nonalactone, 3-(methylthio) propanol, 4-hydroxy-2,5-dimethyl-3(2H)-furanone, 2-ethyl-4-hydroxy-5-methyl-3(2H)-furanone, sotolon, vanillin, guaiacol, and eugenol. Quantitative analysis and calculated odor activity value (OAV) suggested that ethyl butanoate, ethyl 2-methylbutanoate, ethyl hexanoate, 3-methylbutyl acetate, ethyl octanoate, ethyl acetate, 2/3-methyl-1-butanol, butanoic acid, 3-methylbutanoic acid, hexanoic acid, β-damascenone, and acetaldehyde could be the major contributors (OAV ≥?5) to Merlot wine aroma.

Lichrolut-EN SPE was generally comparable with LLE to obtain wine aroma compounds. SPE extract had higher flavor dilution values for esters, and lower for acids and some alcohols than LLE, which is advantageous to study aroma-active compounds in alcoholic beverages due to less interferences from alcohol and acid and improved chromatography. This study confirmed the applicability of LiChrolut-EN SPE as an alternative extraction method for wine aroma characterization to improve laboratory efficiency and minimize the usage of organic solvent.

由于葡萄酒样品中含有高浓度的醇类,因此选择最佳的方法提取和分析葡萄酒中香气活性物质一直是一个挑战。本研究的目的是研究基于lichrolut - en的固相萃取法(SPE)在葡萄酒香气提取中的适用性,并对梅洛葡萄酒中的香气活性化合物进行表征。采用Lichrolut-EN固相萃取或液液萃取(LLE) -溶剂辅助风味蒸发(SAFE)法提取梅洛葡萄酒中的挥发性化合物。两种提取物均用于香气提取稀释分析(AEDA)。对选定的芳香活性化合物进行定量分析,并计算其气味活性值(OAV)。固相萃取和液相色谱萃取均可用于香气提取液稀释分析。在梅洛葡萄酒中具有高稀释度(FD≥?64)值的气味剂有:2-甲基丙酸乙酯、丁酸乙酯、2-甲基丁酸乙酯、3-甲基丁酸乙酯、辛酸乙酯、3-甲基乙酸丁酯、2- 3-甲基丁醇、2-苯乙醇、乙酸、2-甲基丙酸、丁酸、3-甲基丁酸、己酸、β-damascenone、E-whiskeylactone、γ-nonalactone、3-(甲基硫)丙醇、4-羟基2,5-二甲基-3(2H)-呋喃酮、2-乙基-4-羟基-5-甲基-3(2H)-呋喃酮、索托隆、香兰素、愈创木酚和丁香酚。定量分析和计算气味活性值(OAV)表明,丁酸乙酯、2-甲基丁酸乙酯、己酸乙酯、乙酸3-甲基丁酯、辛酸乙酯、乙酸乙酯、2- 3-甲基-1-丁醇、丁酸、3-甲基丁酸、己酸、β-达马酮和乙醛可能是影响梅洛葡萄酒香气的主要因素(OAV≥- 5)。Lichrolut-EN SPE与LLE在获得葡萄酒香气化合物方面基本相当。与LLE相比,SPE萃取物对酯类的香味稀释值更高,对酸类和部分醇类的香味稀释值更低,由于受醇类和酸类的干扰更小,而且色谱法的改进,有利于研究酒精饮料中具有香味活性的化合物。本研究证实了LiChrolut-EN SPE作为葡萄酒香气表征的一种替代提取方法的适用性,可以提高实验室效率,减少有机溶剂的使用。
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引用次数: 28
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Chemosensory Perception
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