Bovine theileriosis, caused by Theileria annulate, is disease affecting cattle and buffaloes worldwide. The current study aimed to screen the blood samples of 30 naturally suspected local buffaloes infected with Theileria species. The blood samples were initially examined by light microscopic and then the positive samples were subjected to PCR reactions. All 30 animals indicated clinical symptoms, such as high fever, loss of appetite, the presence of the hard tick, and enlargement of lymph nodes. The amplified products of 18S rRNA were analyzed, along with molecular detection of the drug-binding site alterations and interrelated changes in the cytochrome b (cyto b) gene. Blood smears revealed the presence of infected erythrocytes with Theileria spp. The PCR results confirmed infection in samples when DNA amplified with partial 18S rRNA and cyto b genes. The sequencing data were obtained from GeneBank using the accession numbers OM937770.1, ON207523.1, ON207525.1, ON207524.1, ON207526.1, and ON207527.1 Following BLAST analysis (Basic Local Alignment Search Tool), genetic differences were observed between the Iraqi isolate OM937770.1 and strains from India, Iran, and Turkey. The data obtained from the current study may reveal the genetic alteration of the local strain in the drug-target codons, which are found in one isolate and are different from the GenBank isolates. The results suggest that the failure of buparvaquone therapy might be due to the resistance to cyto b gene.
{"title":"Phylogenetic Analysis and Detection of Drug Resistance Gene in Theileria annulata Isolated from Buffaloes","authors":"Shehala Rasool Fadel, Howaida Hamel Abed, Amer Rasool Alhaboub","doi":"10.54203/scil.2023.wvj34","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj34","url":null,"abstract":"Bovine theileriosis, caused by Theileria annulate, is disease affecting cattle and buffaloes worldwide. The current study aimed to screen the blood samples of 30 naturally suspected local buffaloes infected with Theileria species. The blood samples were initially examined by light microscopic and then the positive samples were subjected to PCR reactions. All 30 animals indicated clinical symptoms, such as high fever, loss of appetite, the presence of the hard tick, and enlargement of lymph nodes. The amplified products of 18S rRNA were analyzed, along with molecular detection of the drug-binding site alterations and interrelated changes in the cytochrome b (cyto b) gene. Blood smears revealed the presence of infected erythrocytes with Theileria spp. The PCR results confirmed infection in samples when DNA amplified with partial 18S rRNA and cyto b genes. The sequencing data were obtained from GeneBank using the accession numbers OM937770.1, ON207523.1, ON207525.1, ON207524.1, ON207526.1, and ON207527.1 Following BLAST analysis (Basic Local Alignment Search Tool), genetic differences were observed between the Iraqi isolate OM937770.1 and strains from India, Iran, and Turkey. The data obtained from the current study may reveal the genetic alteration of the local strain in the drug-target codons, which are found in one isolate and are different from the GenBank isolates. The results suggest that the failure of buparvaquone therapy might be due to the resistance to cyto b gene.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41796223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.54203/scil.2023.wvj27
A. Nikkhah, M. Alimirzaei
The transition phase is thought to be the most critical period in high-producing dairy cows’ productive cycle. Maternal stresses during the peripartum period affect both dam and newborn calf health. The objective of this review article was to describe the role of prepartal metabolic and environmental stressors on postpartum-related disorders in both cows and newborn calves. The transition phase (21 d before to 21 d after calving) is considered the most critical period in dairy cattle life. Decreased dry matter intake coincides with increased nutrient demands due to the onset of lactation. It can lead to negative energy balance and tremendous metabolic challenges for high-producing dairy cows. During this time, insulin concentrations and peripheral tissue sensitivity decrease, leading to fat mobilization from adipose tissue. Such incidences would increase levels of non-esterified fatty acids and beta-hydroxybutyric acid in plasma. The success in the transition from the dry to lactating phase depends on how the cow could deal with such challenges. Failure to handle such metabolic alterations may predispose cattle to costly metabolic disorders such as ketosis, fatty liver, retained placenta, displacement abomasum, and infectious diseases, including metritis and mastitis in the postpartum period. The energy content of dairy cow diets in the dry-off (40 days before calving) or close-up (21 days before calving) periods may influence cow physiology and health in the peripartum period. The role of a transition period diet on cow health and productivity in the early or overall lactation period has been frequently investigated. However, the role of stressors such as nutritional deficiencies, heat stress, stocking density, and grouping in the late gestation period on the health and performance of cows and calves are much less addressed. Therefore, the present review delineates and reviews updates on the role of maternal stresses during the peripartum period on dam as well as newborn calf physiology and behavior.
{"title":"Management Updates on Prepartal Stress Effects on Transition Cow and Calf Health","authors":"A. Nikkhah, M. Alimirzaei","doi":"10.54203/scil.2023.wvj27","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj27","url":null,"abstract":"The transition phase is thought to be the most critical period in high-producing dairy cows’ productive cycle. Maternal stresses during the peripartum period affect both dam and newborn calf health. The objective of this review article was to describe the role of prepartal metabolic and environmental stressors on postpartum-related disorders in both cows and newborn calves. The transition phase (21 d before to 21 d after calving) is considered the most critical period in dairy cattle life. Decreased dry matter intake coincides with increased nutrient demands due to the onset of lactation. It can lead to negative energy balance and tremendous metabolic challenges for high-producing dairy cows. During this time, insulin concentrations and peripheral tissue sensitivity decrease, leading to fat mobilization from adipose tissue. Such incidences would increase levels of non-esterified fatty acids and beta-hydroxybutyric acid in plasma. The success in the transition from the dry to lactating phase depends on how the cow could deal with such challenges. Failure to handle such metabolic alterations may predispose cattle to costly metabolic disorders such as ketosis, fatty liver, retained placenta, displacement abomasum, and infectious diseases, including metritis and mastitis in the postpartum period. The energy content of dairy cow diets in the dry-off (40 days before calving) or close-up (21 days before calving) periods may influence cow physiology and health in the peripartum period. The role of a transition period diet on cow health and productivity in the early or overall lactation period has been frequently investigated. However, the role of stressors such as nutritional deficiencies, heat stress, stocking density, and grouping in the late gestation period on the health and performance of cows and calves are much less addressed. Therefore, the present review delineates and reviews updates on the role of maternal stresses during the peripartum period on dam as well as newborn calf physiology and behavior.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46375669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.54203/scil.2023.wvj32
Lailatun Nisfimawardah, A. Firmawati, Muhammad Nur Ihsan, T. Susilawati, S. Wahjuningsih
The success of artificial insemination (AI) in small ruminants, especially goats, depends on the quality of frozen semen. Therefore, the current study aimed to determine the quality of various diluents, including tris-egg yolk, AndroMed®, and OviXcell®, on semen quality. The fresh semen samples from three male Saanen goats aged 1.5-2 years were collected and the mean individual motility of samples was recorded at 70%. The cryopreservation quality of the semen was evaluated based on motility, viability, abnormality, and total sperm motility (TSM) indexes. The present laboratory experiment was performed with 3 treatments and 10 repetitions. The treatments in this study were T0 (tris-egg yolk), T1 (AndroMed®), and T2 (OviXcell®). The results showed no significant difference in the parameters of motility, viability, abnormality, and TSM among the treatment group. The kinematic parameters’ average path length, velocity curved linear, and linearity showed a significant difference in all treatment groups. However, there were no significant differences among the three groups in terms of motility, progressiveness, distance curved line, distance straight line, average velocity path, velocity straight line, straightness, amplitude lateral head, beat cross frequency, and wobble kinematic parameters. Motility was higher in T2 than in T0 and T1, viability was higher in T1 than in T0 and T2, and abnormality was lower in T1 than in T0 and T2. In conclusion, the use of various diluents, such as tris-egg yolk, AndroMed®, and OviXcell®, can maintain the quality of frozen spermatozoa for over 24 hours, including motility, viability, abnormality, and TSM. Kinematic parameters obtained using CASA IVOS II can provide relevant information for various parameters using these diluents.
{"title":"Semen Cryopreservation Quality and Sperm Kinematics of Saanen Goats Using Different Diluents","authors":"Lailatun Nisfimawardah, A. Firmawati, Muhammad Nur Ihsan, T. Susilawati, S. Wahjuningsih","doi":"10.54203/scil.2023.wvj32","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj32","url":null,"abstract":"The success of artificial insemination (AI) in small ruminants, especially goats, depends on the quality of frozen semen. Therefore, the current study aimed to determine the quality of various diluents, including tris-egg yolk, AndroMed®, and OviXcell®, on semen quality. The fresh semen samples from three male Saanen goats aged 1.5-2 years were collected and the mean individual motility of samples was recorded at 70%. The cryopreservation quality of the semen was evaluated based on motility, viability, abnormality, and total sperm motility (TSM) indexes. The present laboratory experiment was performed with 3 treatments and 10 repetitions. The treatments in this study were T0 (tris-egg yolk), T1 (AndroMed®), and T2 (OviXcell®). The results showed no significant difference in the parameters of motility, viability, abnormality, and TSM among the treatment group. The kinematic parameters’ average path length, velocity curved linear, and linearity showed a significant difference in all treatment groups. However, there were no significant differences among the three groups in terms of motility, progressiveness, distance curved line, distance straight line, average velocity path, velocity straight line, straightness, amplitude lateral head, beat cross frequency, and wobble kinematic parameters. Motility was higher in T2 than in T0 and T1, viability was higher in T1 than in T0 and T2, and abnormality was lower in T1 than in T0 and T2. In conclusion, the use of various diluents, such as tris-egg yolk, AndroMed®, and OviXcell®, can maintain the quality of frozen spermatozoa for over 24 hours, including motility, viability, abnormality, and TSM. Kinematic parameters obtained using CASA IVOS II can provide relevant information for various parameters using these diluents.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42558781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.54203/scil.2023.wvj36
T. Ahamed, Papia Sultana, Md. Zaminur Rahman, Palash Bose, M. Rafiqul Islam, M. Khatun, Md. Ariful Islam
Duck plague (DP) or duck viral enteritis is a fatal viral disease of ducks that causes huge economic losses in the duck industry. The present study was performed to determine the immune response and protective efficacy of an inactivated DP vaccine prepared from a local virulent DP virus. A virulent DP virus was obtained from the laboratory repository of the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh (Bangladesh). The DP virus (EID50 105.3/ml) was inactivated using 0.04% formalin. The alum (40 g/L) was added to the inactivated DP virus as an adjuvant. A total of 60 Khaki Campbell male ducks aged 17 weeks were randomly divided into three groups. Ducks of groups A (n = 20) and B (n = 20) were vaccinated intramuscularly in the breast muscle with 1 ml of inactivated DP vaccine and a live attenuated DP vaccine, respectively. Ducks of group C (n = 20) were kept as unvaccinated control. Booster vaccination was administered at 2 weeks after primary vaccination. Antibody titers of vaccinated ducks were measured at 7, 14, 21, and 28 days post-vaccination (DPV) using a passive haemagglutination (PHA) test. Ducks of both vaccinated and unvaccinated groups were challenged with 1 ml virulent DP virus (EID50 104.3/ml) at 28 DPV. Clinical signs, morbidity and mortality, and gross pathological lesions of vaccinated and control ducks were observed for 10 days post-challenge to evaluate the protective efficacy of inactivated DP vaccine. The mean PHA antibody titers of vaccinated ducks of group A at 7, 14, 21, and 28 DPV were 5 ± 0.43, 26 ± 1.71, 43 ± 3.4, and 54 ± 3.28, respectively. Ducks in group B had mean serum PHA antibody titers of 21 ± 1.71, 41 ± 3.28, 52 ± 3.41, and 84 ± 7.25 at 7, 14, 21, and 28 DPV, respectively. No mortality or gross pathological lesions were observed in vaccinated ducks after they were subjected to a challenge infection. Additionally, no significant difference was observed between groups A and B in terms of the challenge infection. The mortality rate of the control group of ducks was 70%. Hemorrhage in the trachea and intestine and necrotic foci in the liver were seen in unvaccinated control ducks (group C). Experimentally developed inactivated DP vaccine induced a protective serum antibody titer and conferred 100% protection against virulent challenge infection up to 10 days observation period.
鸭瘟(DP)或鸭病毒性肠炎是一种致命的鸭子病毒性疾病，给养鸭业造成巨大的经济损失。本研究旨在确定由当地DP毒力病毒制备的DP灭活疫苗的免疫应答和保护效果。从Mymensingh(孟加拉国)孟加拉国农业大学微生物学和卫生系的实验室库中获得了一种致命性DP病毒。0.04%福尔马林灭活DP病毒(EID50 105.3/ml)。将明矾(40 g/L)加入灭活的DP病毒中作为佐剂。选取17周龄卡其坎贝尔公鸭60只，随机分为3组。A组(n = 20)和B组(n = 20)分别在胸肌肌内接种1 ml DP灭活疫苗和1 ml DP减毒活疫苗。C组(n = 20)作为未接种疫苗的对照组。初次接种后2周进行加强接种。采用被动血凝(PHA)试验，分别在接种后7、14、21和28天检测接种鸭的抗体滴度。接种疫苗组和未接种疫苗组的鸭在28 DPV时均被1 ml毒力DP病毒(EID50 104.3/ml)攻毒。在攻毒后10 d观察接种鸭和对照鸭的临床症状、发病率和死亡率以及大体病理病变，以评价DP灭活疫苗的保护效果。A组接种疫苗鸭在7、14、21和28 DPV时的平均PHA抗体滴度分别为5±0.43、26±1.71、43±3.4和54±3.28。B组鸭在7、14、21和28 DPV时的平均血清PHA抗体滴度分别为21±1.71、41±3.28、52±3.41和84±7.25。未观察到接种疫苗的鸭在遭受攻毒感染后死亡或大体病理病变。此外，在攻毒感染方面，A组和B组之间没有显著差异。对照组鸭的死亡率为70%。未接种疫苗的对照鸭(C组)出现了气管和肠道出血以及肝脏坏死灶。实验开发的DP灭活疫苗可诱导保护性血清抗体滴度，并在10天的观察期内对毒攻感染提供100%的保护。
{"title":"Protection of Khaki Campbell Ducks against Duck Plague Using an Inactivated Duck Plague Vaccine","authors":"T. Ahamed, Papia Sultana, Md. Zaminur Rahman, Palash Bose, M. Rafiqul Islam, M. Khatun, Md. Ariful Islam","doi":"10.54203/scil.2023.wvj36","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj36","url":null,"abstract":"Duck plague (DP) or duck viral enteritis is a fatal viral disease of ducks that causes huge economic losses in the duck industry. The present study was performed to determine the immune response and protective efficacy of an inactivated DP vaccine prepared from a local virulent DP virus. A virulent DP virus was obtained from the laboratory repository of the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh (Bangladesh). The DP virus (EID50 105.3/ml) was inactivated using 0.04% formalin. The alum (40 g/L) was added to the inactivated DP virus as an adjuvant. A total of 60 Khaki Campbell male ducks aged 17 weeks were randomly divided into three groups. Ducks of groups A (n = 20) and B (n = 20) were vaccinated intramuscularly in the breast muscle with 1 ml of inactivated DP vaccine and a live attenuated DP vaccine, respectively. Ducks of group C (n = 20) were kept as unvaccinated control. Booster vaccination was administered at 2 weeks after primary vaccination. Antibody titers of vaccinated ducks were measured at 7, 14, 21, and 28 days post-vaccination (DPV) using a passive haemagglutination (PHA) test. Ducks of both vaccinated and unvaccinated groups were challenged with 1 ml virulent DP virus (EID50 104.3/ml) at 28 DPV. Clinical signs, morbidity and mortality, and gross pathological lesions of vaccinated and control ducks were observed for 10 days post-challenge to evaluate the protective efficacy of inactivated DP vaccine. The mean PHA antibody titers of vaccinated ducks of group A at 7, 14, 21, and 28 DPV were 5 ± 0.43, 26 ± 1.71, 43 ± 3.4, and 54 ± 3.28, respectively. Ducks in group B had mean serum PHA antibody titers of 21 ± 1.71, 41 ± 3.28, 52 ± 3.41, and 84 ± 7.25 at 7, 14, 21, and 28 DPV, respectively. No mortality or gross pathological lesions were observed in vaccinated ducks after they were subjected to a challenge infection. Additionally, no significant difference was observed between groups A and B in terms of the challenge infection. The mortality rate of the control group of ducks was 70%. Hemorrhage in the trachea and intestine and necrotic foci in the liver were seen in unvaccinated control ducks (group C). Experimentally developed inactivated DP vaccine induced a protective serum antibody titer and conferred 100% protection against virulent challenge infection up to 10 days observation period.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46395254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-25DOI: 10.54203/scil.2023.wvj17
The development of antimicrobial resistance has become a severe global public health emergency. Foods of animal origin are considered possible drivers of resistant bacteria, including Escherichia coli (E. coli) and Salmonella spp. It is associated with the indiscriminate use of antibiotics, resulting in the inability to treat patients infected with antibiotic-resistant pathogens and a high risk of transmission of these resistant pathogens. The current study aimed to determine the prevalence and antibiotic resistance of E. coli and Salmonella spp. in raw table eggs in Morocco. A total of 870 table eggs resulting from 290 samples (3 eggs = 1 sample), were purchased from ambulatory sellers, street vendors, kiosks, and neighborhood markets from different cities in Morocco and transferred to the laboratory in the Hassan II Agronomy and Veterinary Medicine Institute of Rabat, Morocco. The egg shells and contents were tested separately then the isolation and identification of bacterial pathogens were performed according to the Moroccan Standard Norms. The bacterial isolates were tested for susceptibility to six commonly used antibiotics, namely nalidixic acid (30 µg), kanamycin (30 µg), gentamycin (15 µg), ciprofloxacin (15 µg), tetracycline (30 µg), and amoxicillin (10 μg). The findings revealed that 38 samples (13%) tested positive for E. coli of which 9% were on egg shells, and 4% were in egg content, while for Salmonella enteritidis (S. enteritidis), 5 samples (2%) tested positive and only in the egg contents. Escherichia coli showed the highest resistance to amoxicillin, followed by tetracycline and nalidixic acid with 92.10%, 84.21%, and 50%, respectively, and was sensitive to ciprofloxacin (84.21%), kanamycin (65.79%), and gentamicin (60.54%). Salmonella enteritidis had the highest resistance against tetracycline (80%), followed by ciprofloxacin and nalidixic acid with 40% each. The highest sensitivity rates of S. enteritidis were for gentamicin, amoxicillin, and kanamycin at 80%, 80%, and 40%, respectively. Given that these resistant bacteria could potentially be transferred to humans through eggs or egg products, it is necessary to employ strict hygiene measures and provide a wise and legal use of antibiotics in animal breeding.
{"title":"Antibiotic Resistance of Escherichia coli and Salmonella Species Isolated from Table Eggs in Morocco","authors":"","doi":"10.54203/scil.2023.wvj17","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj17","url":null,"abstract":"The development of antimicrobial resistance has become a severe global public health emergency. Foods of animal origin are considered possible drivers of resistant bacteria, including Escherichia coli (E. coli) and Salmonella spp. It is associated with the indiscriminate use of antibiotics, resulting in the inability to treat patients infected with antibiotic-resistant pathogens and a high risk of transmission of these resistant pathogens. The current study aimed to determine the prevalence and antibiotic resistance of E. coli and Salmonella spp. in raw table eggs in Morocco. A total of 870 table eggs resulting from 290 samples (3 eggs = 1 sample), were purchased from ambulatory sellers, street vendors, kiosks, and neighborhood markets from different cities in Morocco and transferred to the laboratory in the Hassan II Agronomy and Veterinary Medicine Institute of Rabat, Morocco. The egg shells and contents were tested separately then the isolation and identification of bacterial pathogens were performed according to the Moroccan Standard Norms. The bacterial isolates were tested for susceptibility to six commonly used antibiotics, namely nalidixic acid (30 µg), kanamycin (30 µg), gentamycin (15 µg), ciprofloxacin (15 µg), tetracycline (30 µg), and amoxicillin (10 μg). The findings revealed that 38 samples (13%) tested positive for E. coli of which 9% were on egg shells, and 4% were in egg content, while for Salmonella enteritidis (S. enteritidis), 5 samples (2%) tested positive and only in the egg contents. Escherichia coli showed the highest resistance to amoxicillin, followed by tetracycline and nalidixic acid with 92.10%, 84.21%, and 50%, respectively, and was sensitive to ciprofloxacin (84.21%), kanamycin (65.79%), and gentamicin (60.54%). Salmonella enteritidis had the highest resistance against tetracycline (80%), followed by ciprofloxacin and nalidixic acid with 40% each. The highest sensitivity rates of S. enteritidis were for gentamicin, amoxicillin, and kanamycin at 80%, 80%, and 40%, respectively. Given that these resistant bacteria could potentially be transferred to humans through eggs or egg products, it is necessary to employ strict hygiene measures and provide a wise and legal use of antibiotics in animal breeding.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43129287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-25DOI: 10.54203/scil.2023.wvj25
The occurrence of feline lower urinary tract disease (FLUD) in Indonesia has not been widely reported. However, the incidence of the disease has increased due to dietary cat patterns. The diet habitually consists of commercial dry food only, without wet food, such as meat. FLUD often affects certain breeds of cats. Surgical therapy is the first option to remove kidney stones; however, rural areas in Indonesia often lack animal surgical facilities. This condition requires alternative therapies to cure the disease. A one-year-old male Angora cat was brought to Rumah Satwa veterinary clinic in Tanah Datar, Indonesia, for examination, with a history of urination difficulties or dysuria, pain in the abdomen when being handled, and lack of desire to mate. A macroscopic examination of urine showed a cloudy and dense appearance. The ultrasound examination revealed a stone (struvite) and a thickening of the urinary bladder wall. The angora cat was diagnosed with obstructive FLUD caused by urolithiasis. A capsule containing 125 mg Keji Beling (Strobilanthes crispus, Bl) extract was administered to the cat once daily to aid the struvite stone dissolution. Keji Beling is a herbal plant easily found in Indonesia and used to treat human kidney stones. After 32 days of therapy, the clinical condition of the cat improved. The ultrasound examination did not find any stones left in the bladder. In conclusion, based on local wisdom, Keji Beling leaves can potentially be an alternative therapy for FLUD in Angora cats with certain conditions.
{"title":"Administration of Strobilanthes crispus in an Angora Cat with Feline Lower Urinary Tract Disease","authors":"","doi":"10.54203/scil.2023.wvj25","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj25","url":null,"abstract":"The occurrence of feline lower urinary tract disease (FLUD) in Indonesia has not been widely reported. However, the incidence of the disease has increased due to dietary cat patterns. The diet habitually consists of commercial dry food only, without wet food, such as meat. FLUD often affects certain breeds of cats. Surgical therapy is the first option to remove kidney stones; however, rural areas in Indonesia often lack animal surgical facilities. This condition requires alternative therapies to cure the disease. A one-year-old male Angora cat was brought to Rumah Satwa veterinary clinic in Tanah Datar, Indonesia, for examination, with a history of urination difficulties or dysuria, pain in the abdomen when being handled, and lack of desire to mate. A macroscopic examination of urine showed a cloudy and dense appearance. The ultrasound examination revealed a stone (struvite) and a thickening of the urinary bladder wall. The angora cat was diagnosed with obstructive FLUD caused by urolithiasis. A capsule containing 125 mg Keji Beling (Strobilanthes crispus, Bl) extract was administered to the cat once daily to aid the struvite stone dissolution. Keji Beling is a herbal plant easily found in Indonesia and used to treat human kidney stones. After 32 days of therapy, the clinical condition of the cat improved. The ultrasound examination did not find any stones left in the bladder. In conclusion, based on local wisdom, Keji Beling leaves can potentially be an alternative therapy for FLUD in Angora cats with certain conditions.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41252324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Motassim El Hanafi, Bouchriti Nourredine, Nassik Saadia, K. Hakim
Several studies have been carried out in Morocco on Salmonella contamination in humans, domestic and wild animals, food products, and the environment. This bacterial genus is responsible for several infections and foodborne illnesses worldwide. The epidemiological situation of contamination by Salmonella is worsened by the development of antibiotic resistance to the main antibiotics used in human and veterinary medicine. The purpose of this study was to review the leading research carried out in this field, emphasizing the antibiotic resistance of this bacterium to antibiotics in humans and animals. Although some studies could not demonstrate the presence of Salmonella in the environments studied, the prevalence of contamination remained relatively high in humans, animals, food products, and the environment. The most critical contaminations were observed in poultry farms and poultry meat. Salmonella causes 42.8% of food poisoning cases in Morocco. It is the second most common cause of poisoning after pesticide poisoning. Morocco ranks first in the Middle East and North Africa for human salmonellosis, with a prevalence of 17.9% (1997-2012). Its prevalence in food products, especially those of animal origin, is very high and could reach 52.9% in turkey meat. Food products have been studied more for their contamination by Salmonella species. Meat products accounted for 17.35% of food poisoning cases. This study revealed that the isolation rate of Salmonella from food products of animal origin was dominated by isolations from meat products, with prevalence rates of 41.76 % from red meat and meat products and 25.88% from poultry meat, followed by prevalence rates of 12.44 % from fish products and 11.80 % from eggs. On the coast of Agadir, the incidence rates of Salmonella were 6.8% and 4.1% in sediment and seawater, respectively. This occurrence was 2.38% in the surface waters of Oued Khoumane. The development of resistance, particularly multi-resistance to antibiotics of therapeutic interest in both humans and animals, is alarming, especially with the ease of transmission of the bacterium to humans and facilitates its dissemination. Research findings indicated that 93.02% of isolates of Salmonella from humans, 79.37% of the strains isolated from poultry, and 46.27% of isolates from food products were resistant to at least one antibiotic.
{"title":"Occurrence of Antibiotic Resistance in Salmonella Serotypes Isolated from Environment, Humans, Animals, and Animal Products in Morocco: A Systematic Review","authors":"Motassim El Hanafi, Bouchriti Nourredine, Nassik Saadia, K. Hakim","doi":"10.54203/scil.2023.wvj4","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj4","url":null,"abstract":"Several studies have been carried out in Morocco on Salmonella contamination in humans, domestic and wild animals, food products, and the environment. This bacterial genus is responsible for several infections and foodborne illnesses worldwide. The epidemiological situation of contamination by Salmonella is worsened by the development of antibiotic resistance to the main antibiotics used in human and veterinary medicine. The purpose of this study was to review the leading research carried out in this field, emphasizing the antibiotic resistance of this bacterium to antibiotics in humans and animals. Although some studies could not demonstrate the presence of Salmonella in the environments studied, the prevalence of contamination remained relatively high in humans, animals, food products, and the environment. The most critical contaminations were observed in poultry farms and poultry meat. Salmonella causes 42.8% of food poisoning cases in Morocco. It is the second most common cause of poisoning after pesticide poisoning. Morocco ranks first in the Middle East and North Africa for human salmonellosis, with a prevalence of 17.9% (1997-2012). Its prevalence in food products, especially those of animal origin, is very high and could reach 52.9% in turkey meat. Food products have been studied more for their contamination by Salmonella species. Meat products accounted for 17.35% of food poisoning cases. This study revealed that the isolation rate of Salmonella from food products of animal origin was dominated by isolations from meat products, with prevalence rates of 41.76 % from red meat and meat products and 25.88% from poultry meat, followed by prevalence rates of 12.44 % from fish products and 11.80 % from eggs. On the coast of Agadir, the incidence rates of Salmonella were 6.8% and 4.1% in sediment and seawater, respectively. This occurrence was 2.38% in the surface waters of Oued Khoumane. The development of resistance, particularly multi-resistance to antibiotics of therapeutic interest in both humans and animals, is alarming, especially with the ease of transmission of the bacterium to humans and facilitates its dissemination. Research findings indicated that 93.02% of isolates of Salmonella from humans, 79.37% of the strains isolated from poultry, and 46.27% of isolates from food products were resistant to at least one antibiotic.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44157278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Niamey region in Niger depends on imports to meet its chicken meat needs. Although consumers appreciate local poultry products, they cannot fulfill their needs. The reluctance of modern producers to use local chickens on their farms is linked to a lack of knowledge of the production characteristics of local strains, which have been little studied. Thus, this study aimed to determine the growth profile of traditional chickens from villages in the Niamey region (Niger). In doing so, 100 local chicks whose parents were collected in the surrounding villages of the Niamey region were followed from hatching until the age of 140 days. The chickens were raised in cages with 10 per compartment of 3 m length and 1.5 m width. Food consumption was recorded daily, and weights were measured weekly. The parameters of the growth curves were obtained using the Gompertz equation. Female and male chickens had a significant weight difference at the third week of age. The mean weight of chicks at hatching was 24.90 ± 0.36 g. At the end of the follow-up, males, with a mean weight of 1523.05 ± 26.22 g were significantly heavier than females (1052.73 ± 14.04 g). Over the entire period of the experiment, the average daily gain and consumption indices were 9.5 g/d and 5.12, respectively. Asymptotic weights were 2096.78 g and 1313.26 g for males and females, respectively. The maturation factor of the Gompertz equation was higher in females (0.0196 g/d) than in males (0.0181 g/d), and the inflection age averaged 75 days for both sexes. In conclusion, Niamey local chickens are slow growing and have a high feed conversion ratio compared to the modern broiler or layer strains.
{"title":"Zootechnical Performance and Growth Curve Modelling of the Niamey Local Chickens in Niger","authors":"Guisso Taffa Adamou, S. Issa, Hamani Bachir, Chaibou Mahamadou, Detilleux Johann, Moula Nassim","doi":"10.54203/scil.2023.wvj19","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj19","url":null,"abstract":"The Niamey region in Niger depends on imports to meet its chicken meat needs. Although consumers appreciate local poultry products, they cannot fulfill their needs. The reluctance of modern producers to use local chickens on their farms is linked to a lack of knowledge of the production characteristics of local strains, which have been little studied. Thus, this study aimed to determine the growth profile of traditional chickens from villages in the Niamey region (Niger). In doing so, 100 local chicks whose parents were collected in the surrounding villages of the Niamey region were followed from hatching until the age of 140 days. The chickens were raised in cages with 10 per compartment of 3 m length and 1.5 m width. Food consumption was recorded daily, and weights were measured weekly. The parameters of the growth curves were obtained using the Gompertz equation. Female and male chickens had a significant weight difference at the third week of age. The mean weight of chicks at hatching was 24.90 ± 0.36 g. At the end of the follow-up, males, with a mean weight of 1523.05 ± 26.22 g were significantly heavier than females (1052.73 ± 14.04 g). Over the entire period of the experiment, the average daily gain and consumption indices were 9.5 g/d and 5.12, respectively. Asymptotic weights were 2096.78 g and 1313.26 g for males and females, respectively. The maturation factor of the Gompertz equation was higher in females (0.0196 g/d) than in males (0.0181 g/d), and the inflection age averaged 75 days for both sexes. In conclusion, Niamey local chickens are slow growing and have a high feed conversion ratio compared to the modern broiler or layer strains.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42052256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-25DOI: 10.54203/scil.2023.wvj22
Tesfaye D Engida, Mihretu Ayele, Hika Waktole, B. Tamir, F. Regassa, T. B. Tufa
Phytogenic feed additives (PFAs) have promising importance in chicken production as antibiotic alternatives to balance chicken gut microorganisms and improve productivity. The objectives of this study were to evaluate the body weight gain (BWG) and gut bacterial load of broiler chicks fed on selected herbs. For this experiment, 360 unsexed one-day-old broiler chicks of Cobb 500 with an average weight of 40.74 g were randomly allocated into six treatment groups with three replicates of 20 chicks in each pen. The treatment 1 (T1) group was fed by a basal diet alone. Chickens of T2, T3, T4, T5, and T6 were fed the basal diet containing 1% of basil, lemongrass, peppermint, rosemary, and thyme leaves powder, respectively for 49 days. Body weight (BW), BWG, and average daily weight gain (ADWG) data were recorded every week and at the end of every phase. On days 21 and 42, three chickens from each replicate were slaughtered for microbiological analysis (pathogenic and normal flora) of cecum contents aseptically. The obtained result showed that chickens kept on T3 had significantly higher BW, BWG, and ADWG during the starter and grower phases. Significantly highest final BW was recorded during the finisher phase on T3 and T6. Chickens that consumed T3 and T6 had significantly higher overall BWG and ADWG. The lowest Escherichia coli counts were seen in chickens fed on rosemary (T5) on both days 21 and 42 of the experimental time. Similarly, the highest Lactobacilli counts were recorded on chicken fed on T5 (day 21) and T3 (day 42). On the other hand, almost all treatment herbs showed a higher Enterococcal count, with the highest recorded for T3 (day 21) and T6 (day 42). The present findings suggest that supplementing lemongrass and thyme leaf powder improves BW performance and gut microbial composition. Likewise, rosemary leaf powder enhances the beneficial microbial composition and reduces pathogenic bacteria. However, the underlying detailed biological mechanisms and dose standardization of these herbs for inclusion in the diet of broiler chickens need to be studied further.
{"title":"Effects of Phytogenic Feed Additives on Body Weight Gain and Gut Bacterial Load in Broiler Chickens","authors":"Tesfaye D Engida, Mihretu Ayele, Hika Waktole, B. Tamir, F. Regassa, T. B. Tufa","doi":"10.54203/scil.2023.wvj22","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj22","url":null,"abstract":"Phytogenic feed additives (PFAs) have promising importance in chicken production as antibiotic alternatives to balance chicken gut microorganisms and improve productivity. The objectives of this study were to evaluate the body weight gain (BWG) and gut bacterial load of broiler chicks fed on selected herbs. For this experiment, 360 unsexed one-day-old broiler chicks of Cobb 500 with an average weight of 40.74 g were randomly allocated into six treatment groups with three replicates of 20 chicks in each pen. The treatment 1 (T1) group was fed by a basal diet alone. Chickens of T2, T3, T4, T5, and T6 were fed the basal diet containing 1% of basil, lemongrass, peppermint, rosemary, and thyme leaves powder, respectively for 49 days. Body weight (BW), BWG, and average daily weight gain (ADWG) data were recorded every week and at the end of every phase. On days 21 and 42, three chickens from each replicate were slaughtered for microbiological analysis (pathogenic and normal flora) of cecum contents aseptically. The obtained result showed that chickens kept on T3 had significantly higher BW, BWG, and ADWG during the starter and grower phases. Significantly highest final BW was recorded during the finisher phase on T3 and T6. Chickens that consumed T3 and T6 had significantly higher overall BWG and ADWG. The lowest Escherichia coli counts were seen in chickens fed on rosemary (T5) on both days 21 and 42 of the experimental time. Similarly, the highest Lactobacilli counts were recorded on chicken fed on T5 (day 21) and T3 (day 42). On the other hand, almost all treatment herbs showed a higher Enterococcal count, with the highest recorded for T3 (day 21) and T6 (day 42). The present findings suggest that supplementing lemongrass and thyme leaf powder improves BW performance and gut microbial composition. Likewise, rosemary leaf powder enhances the beneficial microbial composition and reduces pathogenic bacteria. However, the underlying detailed biological mechanisms and dose standardization of these herbs for inclusion in the diet of broiler chickens need to be studied further.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41752751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-25DOI: 10.54203/scil.2023.wvj16
S. Hachim, Herve Tchoffo, Mingoas Kilekoung Jean-Pierre, Dorice Kana Azafack, F. Ngoula
A cow can give birth to an average of 6-7 calves in her entire reproductive period. The remaining oocytes could be used for the in vitro production of embryos. The present study was conducted to evaluate the effects of ovarian and non-ovarian factors on the follicular population and oocyte maturation of three Chadian cattle breeds (Arab, Kouri, and Toupouri). For this purpose, the ovaries of 166 cycled cows were collected at the Farcha slaughterhouse of Chad and placed individually in labeled conical tubes containing 0.9% NaCl and 0.5 mg/ml penicillin-streptomycin. After clearing the ovaries of tissue debris, they were weighed, and the follicles were counted. The diameter of each follicle was measured and classified into three categories. A total of 2734 oocytes were collected in 28 days with a minimum of 97 per day by the slicing method using a 10X stereoscope. They were then classified into four groups according to the structure of their cumulus oophorus. Immature oocytes (class 1 and 2 [1455]) were placed in different culture media consisting of Minimum Essential Medium (MEM) alone, MEM with 10% follicular fluid, and MEM with 50% follicular fluid for oocyte maturation. The results indicated that the mean follicular population and mean oocyte yield were 24.71 ± 0.88 and 11.65 ± 0.94, respectively. The mean oocyte index and the number of cultivable oocytes for in vitro embryo production (class 1 and 2) were 1.03 ± 0.23 and 1.65 ± 0.94, respectively. The number of follicles observed in the age group of 6-9 years was higher than in other age groups. Oocyte yield was significantly higher in cows with a body condition score of 4-5 compared to average and lean cows. Among the different culture media used for oocyte maturation, the medium consisting of MEM plus 10% follicular fluid recorded a higher maturation rate than the other culture media. Cows aged 6-9 years had a higher maturation rate than other age groups. In conclusion, the good follicle (follicle that produced oocyte) and appropriate oocyte performance were observed in cows with body condition score 3-5 and an age range of 6-9 years.
{"title":"Investigation of Ovarian and Non-ovarian Associated Factors Related to Follicular Population and Oocyte Maturation of Chadian Cattle Breeds","authors":"S. Hachim, Herve Tchoffo, Mingoas Kilekoung Jean-Pierre, Dorice Kana Azafack, F. Ngoula","doi":"10.54203/scil.2023.wvj16","DOIUrl":"https://doi.org/10.54203/scil.2023.wvj16","url":null,"abstract":"A cow can give birth to an average of 6-7 calves in her entire reproductive period. The remaining oocytes could be used for the in vitro production of embryos. The present study was conducted to evaluate the effects of ovarian and non-ovarian factors on the follicular population and oocyte maturation of three Chadian cattle breeds (Arab, Kouri, and Toupouri). For this purpose, the ovaries of 166 cycled cows were collected at the Farcha slaughterhouse of Chad and placed individually in labeled conical tubes containing 0.9% NaCl and 0.5 mg/ml penicillin-streptomycin. After clearing the ovaries of tissue debris, they were weighed, and the follicles were counted. The diameter of each follicle was measured and classified into three categories. A total of 2734 oocytes were collected in 28 days with a minimum of 97 per day by the slicing method using a 10X stereoscope. They were then classified into four groups according to the structure of their cumulus oophorus. Immature oocytes (class 1 and 2 [1455]) were placed in different culture media consisting of Minimum Essential Medium (MEM) alone, MEM with 10% follicular fluid, and MEM with 50% follicular fluid for oocyte maturation. The results indicated that the mean follicular population and mean oocyte yield were 24.71 ± 0.88 and 11.65 ± 0.94, respectively. The mean oocyte index and the number of cultivable oocytes for in vitro embryo production (class 1 and 2) were 1.03 ± 0.23 and 1.65 ± 0.94, respectively. The number of follicles observed in the age group of 6-9 years was higher than in other age groups. Oocyte yield was significantly higher in cows with a body condition score of 4-5 compared to average and lean cows. Among the different culture media used for oocyte maturation, the medium consisting of MEM plus 10% follicular fluid recorded a higher maturation rate than the other culture media. Cows aged 6-9 years had a higher maturation rate than other age groups. In conclusion, the good follicle (follicle that produced oocyte) and appropriate oocyte performance were observed in cows with body condition score 3-5 and an age range of 6-9 years.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71009778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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