Pub Date : 2022-12-25DOI: 10.54203/scil.2022.wvj56
M. Alimirzaei, A. Nikkhah
Despite many efforts to control and treat neonatal calf diarrhea (NCD), it remains the primary cause of calf mortality in dairy herds worldwide. The objective of this article was to develop and discuss an empirical therapeutic protocol to save newborn calves with severe diarrhea. The pathophysiology of diarrhea has been well described previously. However, there is a significant gap between scientific findings and practical implementations. Reducing the number of calves with failure of passive transfer, regular sanitation of the calf environment, and optimal dry cow nutrition and management are fundamental measures in controlling diarrhea in commercial settings. As such, optimizing colostrum feeding management and improving ambiance hygiene are among the most important management practices to prevent calf diarrhea. Nonetheless, the occurrence of NCD would be unavoidable due to its multifactorial nature and pathophysiology. According to the degree of dehydration and general appearance of ill calves (e.g., degree of sunken eye and loss of suck reflex), NCD can be classified into mild to severe cases. Early diagnosis and treatment of both mild and severe cases could reduce pathogens shedding into the calf environment. Notably, diarrhea treatment needs profound scientific farm education and mentoring regarding the physiology of NCD. Since a variety of organisms, such as bacteria, viruses, and protozoa, may be responsible for NCD, it is evident that reliable diagnosis requires optimal sampling and laboratory analysis. However, waiting for laboratory results may waste the golden time of treatment. Therefore, rapid and decisive treatment would be mandatory, especially in severely infected calves or sepsis cases. Accordingly, an effective aggressive treatment protocol was developed and discussed in this article as the last chance to keep diarrheic calves alive.
{"title":"Development of an Aggressive Treatment Protocol against Neonatal Calf Diarrhea: The Last Chance to Rescue Severely Infected Calves","authors":"M. Alimirzaei, A. Nikkhah","doi":"10.54203/scil.2022.wvj56","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj56","url":null,"abstract":"Despite many efforts to control and treat neonatal calf diarrhea (NCD), it remains the primary cause of calf mortality in dairy herds worldwide. The objective of this article was to develop and discuss an empirical therapeutic protocol to save newborn calves with severe diarrhea. The pathophysiology of diarrhea has been well described previously. However, there is a significant gap between scientific findings and practical implementations. Reducing the number of calves with failure of passive transfer, regular sanitation of the calf environment, and optimal dry cow nutrition and management are fundamental measures in controlling diarrhea in commercial settings. As such, optimizing colostrum feeding management and improving ambiance hygiene are among the most important management practices to prevent calf diarrhea. Nonetheless, the occurrence of NCD would be unavoidable due to its multifactorial nature and pathophysiology. According to the degree of dehydration and general appearance of ill calves (e.g., degree of sunken eye and loss of suck reflex), NCD can be classified into mild to severe cases. Early diagnosis and treatment of both mild and severe cases could reduce pathogens shedding into the calf environment. Notably, diarrhea treatment needs profound scientific farm education and mentoring regarding the physiology of NCD. Since a variety of organisms, such as bacteria, viruses, and protozoa, may be responsible for NCD, it is evident that reliable diagnosis requires optimal sampling and laboratory analysis. However, waiting for laboratory results may waste the golden time of treatment. Therefore, rapid and decisive treatment would be mandatory, especially in severely infected calves or sepsis cases. Accordingly, an effective aggressive treatment protocol was developed and discussed in this article as the last chance to keep diarrheic calves alive.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48458206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-25DOI: 10.54203/scil.2022.wvj52
O. Kandil, Fatma Badawy Aboelwafa, Esraa Ismail, S. M. Kandeel, N. Ghanem, Abd El-Kader Gamal El-Din
In vitro embryo generation, cryopreservation, and embryo transfer are examples of assisted reproductive technologies that can be used to improve camel genetic performance and fertility. The aim of this study was to investigate the impact of ascorbic acid supplementation to in vitro maturation media on the maturation rate, morphology, and gene expression of fresh and vitrified in vitro matured dromedary camel oocytes. In the current study, 810 oocytes of excellent and good quality were in vitro matured in maturation medium (TCM-199 + 10 ug/ml follicle stimulated hormone + 10% fetal calf serum + 100 IU/ml Pregnant mare serum + 50 μg/ml gentamycin) without any additives to act as a control group (C) and with 50 μg/ml ascorbic acid group (AA) and incubation in a CO2 incubator (38.5 ̊C, 5% CO2, 20% O2 and 95% humidity) for 40 hours. In vitro matured dromedary camel oocytes with the first polar body (n = 210) in C group and AA group (n = 250) were placed in basic medium (BM) and then placed in vitrification solution1 (VS1) for one minute, followed by the transfer of oocytes to VS2 (double concentration of VS1, containing 20% Ethyl Glycol (EG) and +20% Dimethyl sulfoxide) for 30 seconds. Oocytes were then loaded into sterile 0.25 ml straws and stored in liquid nitrogen for 7-10 days. The normal fresh and vitrified /thawed in vitro matured dromedary camel oocytes were kept in RNA later at a -80°C freezer for gene expression analysis. The maturation rate of dromedary camel oocytes in the in vitro matured AA group was significantly higher than that of the C group. The percentage of normally recovered vitrified/thawed oocytes was higher in the in vitro matured with ascorbic acid (VAA) than in the control (VC) group. The expression pattern of the SOD1 gene and GDF9 gene was upregulated in fresh AA and VAA groups than in the fresh C and VC groups. The profile of the SOD1 gene was more abundant in the vitrified/thawed oocytes VAA group than in the VC group. All vitrified/thawed groups, whether control or ascorbic acid supplemented, had lower levels of SOD1, GDF9, and BMP15 expression, compared to the fresh groups. In conclusion, the supplementation of the maturation medium with ascorbic acid has an increased maturation rate, and normal morphology of vitrified/ thawed oocytes which was linked with upregulation of SOD1, GDF9 genes expression.
{"title":"Effects of Ascorbic Acid on Maturation Rate, Morphology, and Gene Expression of Vitrified In Vitro Matured Dromedary Camel Oocytes","authors":"O. Kandil, Fatma Badawy Aboelwafa, Esraa Ismail, S. M. Kandeel, N. Ghanem, Abd El-Kader Gamal El-Din","doi":"10.54203/scil.2022.wvj52","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj52","url":null,"abstract":"In vitro embryo generation, cryopreservation, and embryo transfer are examples of assisted reproductive technologies that can be used to improve camel genetic performance and fertility. The aim of this study was to investigate the impact of ascorbic acid supplementation to in vitro maturation media on the maturation rate, morphology, and gene expression of fresh and vitrified in vitro matured dromedary camel oocytes. In the current study, 810 oocytes of excellent and good quality were in vitro matured in maturation medium (TCM-199 + 10 ug/ml follicle stimulated hormone + 10% fetal calf serum + 100 IU/ml Pregnant mare serum + 50 μg/ml gentamycin) without any additives to act as a control group (C) and with 50 μg/ml ascorbic acid group (AA) and incubation in a CO2 incubator (38.5 ̊C, 5% CO2, 20% O2 and 95% humidity) for 40 hours. In vitro matured dromedary camel oocytes with the first polar body (n = 210) in C group and AA group (n = 250) were placed in basic medium (BM) and then placed in vitrification solution1 (VS1) for one minute, followed by the transfer of oocytes to VS2 (double concentration of VS1, containing 20% Ethyl Glycol (EG) and +20% Dimethyl sulfoxide) for 30 seconds. Oocytes were then loaded into sterile 0.25 ml straws and stored in liquid nitrogen for 7-10 days. The normal fresh and vitrified /thawed in vitro matured dromedary camel oocytes were kept in RNA later at a -80°C freezer for gene expression analysis. The maturation rate of dromedary camel oocytes in the in vitro matured AA group was significantly higher than that of the C group. The percentage of normally recovered vitrified/thawed oocytes was higher in the in vitro matured with ascorbic acid (VAA) than in the control (VC) group. The expression pattern of the SOD1 gene and GDF9 gene was upregulated in fresh AA and VAA groups than in the fresh C and VC groups. The profile of the SOD1 gene was more abundant in the vitrified/thawed oocytes VAA group than in the VC group. All vitrified/thawed groups, whether control or ascorbic acid supplemented, had lower levels of SOD1, GDF9, and BMP15 expression, compared to the fresh groups. In conclusion, the supplementation of the maturation medium with ascorbic acid has an increased maturation rate, and normal morphology of vitrified/ thawed oocytes which was linked with upregulation of SOD1, GDF9 genes expression.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44717758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-25DOI: 10.54203/scil.2022.wvj46
Ayman Ameen Samy, A. Arafa, R. H. Hedia, E. S. Ibrahim
Food of animal origin is considered a primary source of foodborne diseases. The misuse of antibiotics to treat and control many bacterial diseases in farm animals has led to multiple antibiotic-resistant pathogens in contaminated food that can seriously threaten public health. The present study aimed to highlight the impact of antimicrobial misuse in Egyptian beef meat, poultry, and dairy farms on the emergence of multiple antibiotic resistance Salmonella and the detection of antibiotic residues in milk. A total of 1050 samples were collected randomly from poultry (liver, intestinal content, and bone marrow), meat, and milk products from different Egyptian governorates. Salmonellae were isolated from the collected samples and subjected to antimicrobial sensitivity testing through disk diffusion test using the most commonly used seven antibiotics in veterinary fields (cefradine, ciprofloxacin, oxytetracycline, erythromycin, amoxicillin, ampicillin, and streptomycin). The detection of oxytetracycline residue in milk samples was performed by high-performance liquid chromatography (HPLC). Most isolated Salmonellae were multiple drug resistant with an incidence rate of 8.6%, 15.4%, and 4% from poultry, meat-associated products, and milk-associated products, respectively, from different governorates. Antibiogram test showed that the isolated Salmonella from poultry, meat, and milk samples were resistant to oxytetracycline at 100%, 31.4%, and 43%, to amoxicillin at 73.3%, 31%, and 50%, and to ampicillin 66.6%, 50%, and 57%, respectively. No resistance to ciprofloxacin was detected in Salmonella isolates from all samples. Using HPLC, oxytetracycline residues were detected in milk samples. In conclusion, more attention should be paid to the connection between the widespread emergence of antibiotic-resistant Salmonella in Egypt and the overuse of antimicrobials in poultry, dairy, and meat farms. This connection affects consumer health and increases the likelihood of resistance genes spreading between different bacterial species.
{"title":"Multiple Drug Resistance Salmonella and Antibiotic Residues in Egyptian Animal Products","authors":"Ayman Ameen Samy, A. Arafa, R. H. Hedia, E. S. Ibrahim","doi":"10.54203/scil.2022.wvj46","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj46","url":null,"abstract":"Food of animal origin is considered a primary source of foodborne diseases. The misuse of antibiotics to treat and control many bacterial diseases in farm animals has led to multiple antibiotic-resistant pathogens in contaminated food that can seriously threaten public health. The present study aimed to highlight the impact of antimicrobial misuse in Egyptian beef meat, poultry, and dairy farms on the emergence of multiple antibiotic resistance Salmonella and the detection of antibiotic residues in milk. A total of 1050 samples were collected randomly from poultry (liver, intestinal content, and bone marrow), meat, and milk products from different Egyptian governorates. Salmonellae were isolated from the collected samples and subjected to antimicrobial sensitivity testing through disk diffusion test using the most commonly used seven antibiotics in veterinary fields (cefradine, ciprofloxacin, oxytetracycline, erythromycin, amoxicillin, ampicillin, and streptomycin). The detection of oxytetracycline residue in milk samples was performed by high-performance liquid chromatography (HPLC). Most isolated Salmonellae were multiple drug resistant with an incidence rate of 8.6%, 15.4%, and 4% from poultry, meat-associated products, and milk-associated products, respectively, from different governorates. Antibiogram test showed that the isolated Salmonella from poultry, meat, and milk samples were resistant to oxytetracycline at 100%, 31.4%, and 43%, to amoxicillin at 73.3%, 31%, and 50%, and to ampicillin 66.6%, 50%, and 57%, respectively. No resistance to ciprofloxacin was detected in Salmonella isolates from all samples. Using HPLC, oxytetracycline residues were detected in milk samples. In conclusion, more attention should be paid to the connection between the widespread emergence of antibiotic-resistant Salmonella in Egypt and the overuse of antimicrobials in poultry, dairy, and meat farms. This connection affects consumer health and increases the likelihood of resistance genes spreading between different bacterial species.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49065205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-25DOI: 10.54203/scil.2022.wvj35
I. N. Sulabda, Anak Agung Gde Oka Dharmayudha, I. Puja
Preservation of sperm by freezing allows breeding dogs that are separated over long distances. To increase the fertility of frozen and then thawed spermatozoa, they must be able to survive the process. The current study aimed to evaluate the sperm motility and DNA integrity of Kintamani dogs extended in extenders with one-step and two-step dilution techniques. Ejaculates collected from four dogs were used in the current study. The semen was divided into two equal parts and diluted with extenders using two different dilution techniques, namely One-step dilution in Tris egg yolk containing 7% glycerol, and a two-step dilution technique diluted in an initial 2:1 with an extender, containing 20% egg yolk without glycerol. The same volume of the second extender was added, including 14% glycerol. The sample was loaded into 0.25 ml straws, cooled to 4°C for 4 hours, equilibrated, and then plunged into the liquid nitrogen. The sperm motility was evaluated using Computer-Assisted Sperm Analysis (CASA), and DNA integrity was assessed using Acridine Orange (AO) stained. Results showed that the sperm motility of Kintamani dogs in extenders using two-step dilution was significantly higher compared to the one-step dilution technique. In addition, the obtained results indicated that two types of dilution steps in Kintamani dog semen were not detrimental to the sperm DNA integrity during the freezing process. In conclusion, extenders with two types of dilution techniques could maintain sperm motility above 30%, and no difference between one and two steps dilution was detected.
{"title":"Comparative Analysis of One-step and Two-step Dilution on Quality of Frozen Semen in Kintamani Dogs","authors":"I. N. Sulabda, Anak Agung Gde Oka Dharmayudha, I. Puja","doi":"10.54203/scil.2022.wvj35","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj35","url":null,"abstract":"Preservation of sperm by freezing allows breeding dogs that are separated over long distances. To increase the fertility of frozen and then thawed spermatozoa, they must be able to survive the process. The current study aimed to evaluate the sperm motility and DNA integrity of Kintamani dogs extended in extenders with one-step and two-step dilution techniques. Ejaculates collected from four dogs were used in the current study. The semen was divided into two equal parts and diluted with extenders using two different dilution techniques, namely One-step dilution in Tris egg yolk containing 7% glycerol, and a two-step dilution technique diluted in an initial 2:1 with an extender, containing 20% egg yolk without glycerol. The same volume of the second extender was added, including 14% glycerol. The sample was loaded into 0.25 ml straws, cooled to 4°C for 4 hours, equilibrated, and then plunged into the liquid nitrogen. The sperm motility was evaluated using Computer-Assisted Sperm Analysis (CASA), and DNA integrity was assessed using Acridine Orange (AO) stained. Results showed that the sperm motility of Kintamani dogs in extenders using two-step dilution was significantly higher compared to the one-step dilution technique. In addition, the obtained results indicated that two types of dilution steps in Kintamani dog semen were not detrimental to the sperm DNA integrity during the freezing process. In conclusion, extenders with two types of dilution techniques could maintain sperm motility above 30%, and no difference between one and two steps dilution was detected.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48106806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-25DOI: 10.54203/scil.2022.wvj31
R. A. Abdullah, Hana Khaleel Ismail, AL-Haaik Ammar Ghanim
Colchicine is a drug widely used for the management of many disorders, such as acute gout and Behçet’s disease. It is also prescribed for the treatment of pericarditis, atrial fibrillation coronary artery diseases, and secondary amyloidosis. In case this drug is used at the early stages of coronavirus infection, its anti-inflammatory properties may reduce the severe inflammatory reactions related to a cytokine storm by affecting the inflammasome. The purpose of the present study was to determine the toxicity of Colchicine on testis in rats from different age groups for 10 days. A total of 27 male Wistar rats were divided into three groups. The rats in group I (control group) were administered distilled water by oral gavage. Group II consisted of young rats (5-6 months old) who orally received Colchicine 3 mg/kg body weight. Group III entailed rats of 14-16 months who were orally administered colchicine 3 mg/kg body weight. The testis of the treated groups was dissected and examined for histological changes and morphometrical analysis. The obtained results indicated that high doses of Colchicine (3 mg/kg body weight) could induce tissue damage to the testis, including degeneration and necrosis of both Sertoli and Leydig cells with irregular divisions of germinal epithelium, even when it was used for short periods (10 days). In the elderly treated rats, there were severe tissue damages, including degeneration and necrosis of germinal epithelium with irregular divisions of germ cells, necrosis of Sertoli and Leydig cells with sloughing of germinal epithelium toward the lumen of the tubule. Therefore, there is a need to conduct more studies to investigate the side effect of Colchicine as it is excessively used in the management of coronavirus.
{"title":"Impact of Colchicine on Histology of Testis in Rats","authors":"R. A. Abdullah, Hana Khaleel Ismail, AL-Haaik Ammar Ghanim","doi":"10.54203/scil.2022.wvj31","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj31","url":null,"abstract":"Colchicine is a drug widely used for the management of many disorders, such as acute gout and Behçet’s disease. It is also prescribed for the treatment of pericarditis, atrial fibrillation coronary artery diseases, and secondary amyloidosis. In case this drug is used at the early stages of coronavirus infection, its anti-inflammatory properties may reduce the severe inflammatory reactions related to a cytokine storm by affecting the inflammasome. The purpose of the present study was to determine the toxicity of Colchicine on testis in rats from different age groups for 10 days. A total of 27 male Wistar rats were divided into three groups. The rats in group I (control group) were administered distilled water by oral gavage. Group II consisted of young rats (5-6 months old) who orally received Colchicine 3 mg/kg body weight. Group III entailed rats of 14-16 months who were orally administered colchicine 3 mg/kg body weight. The testis of the treated groups was dissected and examined for histological changes and morphometrical analysis. The obtained results indicated that high doses of Colchicine (3 mg/kg body weight) could induce tissue damage to the testis, including degeneration and necrosis of both Sertoli and Leydig cells with irregular divisions of germinal epithelium, even when it was used for short periods (10 days). In the elderly treated rats, there were severe tissue damages, including degeneration and necrosis of germinal epithelium with irregular divisions of germ cells, necrosis of Sertoli and Leydig cells with sloughing of germinal epithelium toward the lumen of the tubule. Therefore, there is a need to conduct more studies to investigate the side effect of Colchicine as it is excessively used in the management of coronavirus.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48078445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-25DOI: 10.54203/scil.2022.wvj43
Tewodros Abere Mekuria, M. Kinde
Live cell imaging has provided great benefits in studying multiple processes and molecular interactions within and/or between cells. This review aimed to describe the common live cell microscopy techniques and briefly explain their principles and applications. A wide range of microscopic techniques, from conventional transmitted light to an array of fluorescence microscopy techniques, including advanced super-resolution techniques, can be applied for live-cell imaging. Transmitted light microscopy uses focused transmitted light that goes through a condenser to achieve a very high illumination on the specimen. On the other hand, fluorescence microscopy uses reflected light to capture images of cells or molecules that have been fluorescently dyed. Techniques for transmitted light microscopy are simple to use but have poor resolution. Although the resolution of fluorescent microscopy techniques is only approximately 200-300 nm, this is nevertheless an improvement over conventional transmitted methods. Conventional light microscopy’s resolution was improved by the introduction of the super-resolution microscopy technology family. These methods “break” the diffraction limit, enabling fluorescence imaging with resolutions up to ten times higher than those possible with traditional methods. Each live cell imaging method has advantages and drawbacks. The primary deciding criteria for choosing the type of microscope are the study’s objectives, previous experience, the researcher’s interests, and financial viability. Hence, a thorough understanding of the technique and application of the various live-cell microscopy methods is paramount in life science studies.
{"title":"Basic Principles and Applications of Live Cell Microscopy Techniques: A Review","authors":"Tewodros Abere Mekuria, M. Kinde","doi":"10.54203/scil.2022.wvj43","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj43","url":null,"abstract":"Live cell imaging has provided great benefits in studying multiple processes and molecular interactions within and/or between cells. This review aimed to describe the common live cell microscopy techniques and briefly explain their principles and applications. A wide range of microscopic techniques, from conventional transmitted light to an array of fluorescence microscopy techniques, including advanced super-resolution techniques, can be applied for live-cell imaging. Transmitted light microscopy uses focused transmitted light that goes through a condenser to achieve a very high illumination on the specimen. On the other hand, fluorescence microscopy uses reflected light to capture images of cells or molecules that have been fluorescently dyed. Techniques for transmitted light microscopy are simple to use but have poor resolution. Although the resolution of fluorescent microscopy techniques is only approximately 200-300 nm, this is nevertheless an improvement over conventional transmitted methods. Conventional light microscopy’s resolution was improved by the introduction of the super-resolution microscopy technology family. These methods “break” the diffraction limit, enabling fluorescence imaging with resolutions up to ten times higher than those possible with traditional methods. Each live cell imaging method has advantages and drawbacks. The primary deciding criteria for choosing the type of microscope are the study’s objectives, previous experience, the researcher’s interests, and financial viability. Hence, a thorough understanding of the technique and application of the various live-cell microscopy methods is paramount in life science studies.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44718020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-25DOI: 10.54203/scil.2022.wvj36
S. Long, N. Hien, Phan Thi Hang, N. T. Hoai, Pham Xuan Bach
Dystocia is a common disorder that can cause harmful health risks to bitch and puppies. The aim of the current study was to evaluate some risk factors related to canine dystocia and the application of treatment methods to 612 diagnosed cases in Gaia Pets Clinic and Resort, Hanoi, Vietnam, from December 2013 to May 2020. The investigated factors comprised age, parity and breed size, and litter size, as well as fetal sex in relation to the proportion of dystocia in female canines. Dystocia was frequently observed in female dogs aged 1-3 years, with rates of 76.1%. The highest proportion of dystocia was found in the first litter group (80.21%). The incidence of dystocia increased as the weight of the dog decreased, and it was prevalent in the small breed (61.93%). Dystocia risk decreased as the litter size increased. The interventions used in this study were medical treatment with the hormone oxytocin (1.8%), surgical management with cesarean section (86.11%), and a combination of oxytocin and cesarean section (12.09%), with the success rates of each treatment method as 100%, 98.86%, and 100%, respectively. Some risk factors, such as age, parity, breed size, and litter size identified in the present research, could be used as prognostic indicators in the veterinary practice to optimize the survival rate of female dogs and puppies.
{"title":"Canine Dystocia: The Risk Factors and Treatment Methods in Dogs of Hanoi, Vietnam","authors":"S. Long, N. Hien, Phan Thi Hang, N. T. Hoai, Pham Xuan Bach","doi":"10.54203/scil.2022.wvj36","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj36","url":null,"abstract":"Dystocia is a common disorder that can cause harmful health risks to bitch and puppies. The aim of the current study was to evaluate some risk factors related to canine dystocia and the application of treatment methods to 612 diagnosed cases in Gaia Pets Clinic and Resort, Hanoi, Vietnam, from December 2013 to May 2020. The investigated factors comprised age, parity and breed size, and litter size, as well as fetal sex in relation to the proportion of dystocia in female canines. Dystocia was frequently observed in female dogs aged 1-3 years, with rates of 76.1%. The highest proportion of dystocia was found in the first litter group (80.21%). The incidence of dystocia increased as the weight of the dog decreased, and it was prevalent in the small breed (61.93%). Dystocia risk decreased as the litter size increased. The interventions used in this study were medical treatment with the hormone oxytocin (1.8%), surgical management with cesarean section (86.11%), and a combination of oxytocin and cesarean section (12.09%), with the success rates of each treatment method as 100%, 98.86%, and 100%, respectively. Some risk factors, such as age, parity, breed size, and litter size identified in the present research, could be used as prognostic indicators in the veterinary practice to optimize the survival rate of female dogs and puppies.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71009686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-25DOI: 10.54203/scil.2022.wvj34
Tawfick Sabah Atia, Edris Abobakr Moustafa, Sabeq Islam Ibrahim
The meat industry increasingly considers meat shelf life as a critical problem. Some essential oils contain antibacterial and antioxidant characteristics that help to keep meat safe. Therefore, the purpose of this study was to evaluate the preservation benefits, including antibacterial and antioxidant properties, of cumin, garlic, and thyme essential oils at 1% on chilled beef meat steaks, as well as their effects on pH, total volatile basic nitrogen (TVBN), thiobarbituric acid (TBA), and related sensory aspects (color, odor, appearance, consistency, and overall acceptability). The results of the current study showed that pretreating beef meat steaks with EOs of cumin, garlic, and thyme at a concentration of 1% effectively reduced levels of APC, coliform count, staph aureus count, TVBN, and TBA while extending shelf life to 12, 15, and 18 days when stored at 4°C. In terms of antibacterial and antioxidant properties, shelf life, and sensory quality on beef meat steaks, the thyme essential oil group outperformed cumin and garlic essential oils. The current study introduced an effective natural preservative alternative that could replace undesirable synthetic substances in the future while also lowering antibiotic resistance.
{"title":"Improving Bacterial, Oxidative, and Sensory Quality of Meat Steaks Using Cumin, Garlic, and Thyme Essential Oils","authors":"Tawfick Sabah Atia, Edris Abobakr Moustafa, Sabeq Islam Ibrahim","doi":"10.54203/scil.2022.wvj34","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj34","url":null,"abstract":"The meat industry increasingly considers meat shelf life as a critical problem. Some essential oils contain antibacterial and antioxidant characteristics that help to keep meat safe. Therefore, the purpose of this study was to evaluate the preservation benefits, including antibacterial and antioxidant properties, of cumin, garlic, and thyme essential oils at 1% on chilled beef meat steaks, as well as their effects on pH, total volatile basic nitrogen (TVBN), thiobarbituric acid (TBA), and related sensory aspects (color, odor, appearance, consistency, and overall acceptability). The results of the current study showed that pretreating beef meat steaks with EOs of cumin, garlic, and thyme at a concentration of 1% effectively reduced levels of APC, coliform count, staph aureus count, TVBN, and TBA while extending shelf life to 12, 15, and 18 days when stored at 4°C. In terms of antibacterial and antioxidant properties, shelf life, and sensory quality on beef meat steaks, the thyme essential oil group outperformed cumin and garlic essential oils. The current study introduced an effective natural preservative alternative that could replace undesirable synthetic substances in the future while also lowering antibiotic resistance.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45520891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-25DOI: 10.54203/scil.2022.wvj40
Athhar Manabi Diansyah, M. Yusuf, A. Toleng, M. Dagong, T. Maulana
The fertility rate of bulls in a breeding program is not only described by the quantity and quality of semen. Factors, such as the interstice factor of the sperm and the plasma component of semen, affect the fertility rate of bulls. The fertility rate can also be determined by identifying the protein content of semen plasma. Therefore, the current study aimed to identify the relationship between seminal plasma protein molecular weight and semen quality of Bali-polled bulls. The study was conducted at the Laboratory of Semen Processing, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia, the Research Center for Applied Zoology, National Research and Innovation Agency, Cibinong, Indonesia and the Laboratory of Animal Biotechnology Center, IPB University, Bogor, Indonesia from November 2021 to January 2022. The samples came from 5 Bali-polled and 5 Bali-horned bulls. Semen collection was conducted twice a week using an artificial vagina. The concentration of seminal plasma protein was determined by the Bradford method of 1D-SDS-PAGE. The study results showed that fresh semen of Bali-polled and Bali-horned bulls was considered a normal category. Seminal plasma proteins of Bali-polled and Bali-horned bulls were classified using 8 bands to categorize molecular weight; 150 kD (IGF-1), 110 kD (A-kinase anchoring protein 3), 93 kD (A-kinase anchoring protein 4), 54-87 kD (Arylsulfatase-a), 44-62 kD (N-Acetyl-ß-Guicosaminidase), 44kD (Phosphoglycerate kinase), 15-30 kD (BSP A1/A2, BSP-A3 and BSP-30 [BSP1, BSP3, and BSP5]) and 12-14 kD (Acidic seminal fluid proteins). The findings indicated that both Bali-polled and Bali-horned bulls could have a high reproductive rate. In conclusion, protein analysis based on molecular weight using 1D-SDS-PAGE can be used as a biomarker for semen quality in Bali-polled bulls. Therefore, evaluating the semen quality with a molecular basis as an additional indicator of superior bull in the selection process is an alternative method.
{"title":"The Expression of Plasma Protein in Bali-polled Bulls Using 1D-SDS-PAGE","authors":"Athhar Manabi Diansyah, M. Yusuf, A. Toleng, M. Dagong, T. Maulana","doi":"10.54203/scil.2022.wvj40","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj40","url":null,"abstract":"The fertility rate of bulls in a breeding program is not only described by the quantity and quality of semen. Factors, such as the interstice factor of the sperm and the plasma component of semen, affect the fertility rate of bulls. The fertility rate can also be determined by identifying the protein content of semen plasma. Therefore, the current study aimed to identify the relationship between seminal plasma protein molecular weight and semen quality of Bali-polled bulls. The study was conducted at the Laboratory of Semen Processing, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia, the Research Center for Applied Zoology, National Research and Innovation Agency, Cibinong, Indonesia and the Laboratory of Animal Biotechnology Center, IPB University, Bogor, Indonesia from November 2021 to January 2022. The samples came from 5 Bali-polled and 5 Bali-horned bulls. Semen collection was conducted twice a week using an artificial vagina. The concentration of seminal plasma protein was determined by the Bradford method of 1D-SDS-PAGE. The study results showed that fresh semen of Bali-polled and Bali-horned bulls was considered a normal category. Seminal plasma proteins of Bali-polled and Bali-horned bulls were classified using 8 bands to categorize molecular weight; 150 kD (IGF-1), 110 kD (A-kinase anchoring protein 3), 93 kD (A-kinase anchoring protein 4), 54-87 kD (Arylsulfatase-a), 44-62 kD (N-Acetyl-ß-Guicosaminidase), 44kD (Phosphoglycerate kinase), 15-30 kD (BSP A1/A2, BSP-A3 and BSP-30 [BSP1, BSP3, and BSP5]) and 12-14 kD (Acidic seminal fluid proteins). The findings indicated that both Bali-polled and Bali-horned bulls could have a high reproductive rate. In conclusion, protein analysis based on molecular weight using 1D-SDS-PAGE can be used as a biomarker for semen quality in Bali-polled bulls. Therefore, evaluating the semen quality with a molecular basis as an additional indicator of superior bull in the selection process is an alternative method.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43234907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-25DOI: 10.54203/scil.2022.wvj30
D. El Akkad, Reem M. Ramadan, Hend M. Auda, Yousra N. Abd El-Hafez, M. El-Bahy, S. Abdel-Radi
Clinicians face significant problems in the diagnosis of zoonotic coenurosis. The current study aimed to develop an improved dot-Enzyme-linked-immunosorbent assay (dot-ELISA) for the diagnosis of zoonotic coenurosis using sheep Coenurus cerebralis scolices purified antigen (CcS-Ag) and to compare the obtained results with those of indirect ELISA and Enzyme-linked immunoelectrotransfer blot technique (EITB). Sera were collected from humans and sheep infected or suspected of infection with Coenurus cerebralis, control cases, and cases infected with other parasites. The CcS-Ag was proved to be the most specific antigen. This antigen was fractionated, and its specific polypeptides against anti-C. cerebralis antibodies (ACc-Ab) were identified using EITB. Fractions at the molecular weight (MW) of 48 and 58 kDa were proved as the only specific ones, eluted from the gel and concentrated, then dotted on the NC sheet as pooled antigen before its evaluation in the diagnosis of infection using dot-ELISA. Dot-ELISA demonstrated absolute 100% sensitivity and 100% specificity as recorded by EITB, compared to both fractions on a nitrocellulose (NC) sheet using surgically proved infected human or sheep sera as a gold standard. Diagnosis by ELISA using crude CcS-Ag revealed similar sensitivity but lower specificity (75%). The diagnostic accuracy of dot-ELISA was proved by comparing its results with postmortem data obtained post slaughtering of 20 suspected sheep and patients investigated by computed tomography (CT) and magnetic resonance imaging (MRI). In conclusion, the selection of specific fractions after EITB to be used in dot-ELISA improved the diagnostic value of the test as a diagnostic tool gathering the benefits of ELISA and EITB.
{"title":"Improved Dot-ELISA Assay Using Purified Sheep Coenurus cerebralis Antigenic Fractions for the Diagnosis of Zoonotic Coenurosis","authors":"D. El Akkad, Reem M. Ramadan, Hend M. Auda, Yousra N. Abd El-Hafez, M. El-Bahy, S. Abdel-Radi","doi":"10.54203/scil.2022.wvj30","DOIUrl":"https://doi.org/10.54203/scil.2022.wvj30","url":null,"abstract":"Clinicians face significant problems in the diagnosis of zoonotic coenurosis. The current study aimed to develop an improved dot-Enzyme-linked-immunosorbent assay (dot-ELISA) for the diagnosis of zoonotic coenurosis using sheep Coenurus cerebralis scolices purified antigen (CcS-Ag) and to compare the obtained results with those of indirect ELISA and Enzyme-linked immunoelectrotransfer blot technique (EITB). Sera were collected from humans and sheep infected or suspected of infection with Coenurus cerebralis, control cases, and cases infected with other parasites. The CcS-Ag was proved to be the most specific antigen. This antigen was fractionated, and its specific polypeptides against anti-C. cerebralis antibodies (ACc-Ab) were identified using EITB. Fractions at the molecular weight (MW) of 48 and 58 kDa were proved as the only specific ones, eluted from the gel and concentrated, then dotted on the NC sheet as pooled antigen before its evaluation in the diagnosis of infection using dot-ELISA. Dot-ELISA demonstrated absolute 100% sensitivity and 100% specificity as recorded by EITB, compared to both fractions on a nitrocellulose (NC) sheet using surgically proved infected human or sheep sera as a gold standard. Diagnosis by ELISA using crude CcS-Ag revealed similar sensitivity but lower specificity (75%). The diagnostic accuracy of dot-ELISA was proved by comparing its results with postmortem data obtained post slaughtering of 20 suspected sheep and patients investigated by computed tomography (CT) and magnetic resonance imaging (MRI). In conclusion, the selection of specific fractions after EITB to be used in dot-ELISA improved the diagnostic value of the test as a diagnostic tool gathering the benefits of ELISA and EITB.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46687604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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