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Prevalence and Morphological Characterization of the Camel Nasal Botfly, Cephalopina titillator (Diptera: Oestridae) Collected from Abattoirs in Egypt 埃及屠宰场骆驼鼻蝇(Cephalopina titillator,双翅目:骆驼鼻蝇科)流行及形态特征
Sherif Hamed, M. Shaalan, E. Khater, Mohamed B Kenawy, E. Ghallab
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引用次数: 0
Molecular Characterization of Escherichia coli Isolated from Children Who Have Acute Diarrhea in Different Iraqi Kurdistan Hospitals 伊拉克库尔德斯坦不同医院急性腹泻患儿大肠杆菌分离分子特征分析
Sardar Ali, M. Babakir-Mina, T. Soor, Hiwa A. Ahmad
Escherichia coli ( E. coli ) is one of the most frequent opportunistic pathogens. We aimed to carry out this study on antimicrobial resistance pattern and phylogenetic background of E. coli isolated from children who have diarrhea in different hospitals of the Iraqi Kurdistan region. A total of 200 fecal samples of children (52.5% male and 47.5% female) were included. Stool samples were cultured on selective media to isolate E. coli bacteria. The antibiotic susceptibility testing and molecular characterization of the isolates were carried out. Antimicrobial susceptibility testing was performed by disc diffusion test disc PCR was used to detect five antimicrobial resistance genes coding for β-lactamases ( bla TEM, and bla CTX , bla OXA , bla SHV , bla CMY) to study phylogenetic grouping of E. coli . The Escherichia coli were highly resistant to β-lactam antibiotics, and the highest resistance was seen to Cefotaxime (45.4%). According to the phylogenetic grouping of E. coli isolates, group B2 was the dominant group and bla OXAgene (57.5%) was the predominant resistance genes among E. coli isolates. This study demonstrated the clinical concern of E. coli in children. The E. coli are highly resistance to common beta-lactam antibiotics and there are high rates of the resistance genes among E. coli isolates in children. An antibiotic surveillance would be useful continuously control and prevention of infectious diarrhea in children.
大肠杆菌是最常见的机会致病菌之一。目的对伊拉克库尔德斯坦地区不同医院腹泻患儿大肠杆菌的耐药模式和系统发育背景进行研究。共纳入200份儿童粪便样本(男52.5%,女47.5%)。粪便样本在选择性培养基上培养分离大肠杆菌。对分离菌株进行了药敏试验和分子鉴定。采用圆盘扩散试验进行抗菌药敏试验,采用圆盘PCR检测5个编码β-内酰胺酶的耐药基因(bla TEM、bla CTX、bla OXA、bla SHV、bla CMY),研究大肠杆菌的系统发育分型。大肠杆菌对β-内酰胺类抗生素耐药程度较高,其中以头孢噻肟耐药最高(45.4%)。根据大肠杆菌的系统发育分组,B2群为优势群,bla OXAgene(57.5%)为优势耐药基因。本研究证明了儿童大肠杆菌的临床关注。大肠杆菌对常见的-内酰胺类抗生素具有高度耐药性,儿童大肠杆菌分离株中耐药基因的比例很高。抗生素监测将有助于持续控制和预防儿童感染性腹泻。
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引用次数: 0
Toxicity of Plant-Based Silver Nanoparticle Against Potential Disease Vector Musca domestica (Diptera: Muscidae) in Tabuk, Saudi Arabia 植物基纳米银颗粒对沙特阿拉伯Tabuk潜在病媒家蝇(双翅目:蝇科)的毒性研究
A. Aziz
The synanthropic house fly, Musca domestica is a major vector for the transmission of deadly diseases from one place to another. It plays a primary role in some diseases and community spread against human and animal origin. As the eradication and control measures are challenging through chemical or synthetic insecticides. The repeated exposure to insecticides developed resistance and undesired effects. A study to find the novel insecticides from natural plant-based origin were developed from Lavandula angustifolia , Citrus limon , Mentha piperita , Azadirachta indica and AgNPs against M. domestica at five different concentrations of each extract. The obtained results revealed good mortality from Citrus limon extract through dipping and feeding techniques with LC 50 values were 296.145 ppm (contact bioassay) and 380.187 ppm (feeding bioassay) after 24 h post-treatment. The plant-based nanoparticles developed from A. indica revealed good activity at a minimal concentration to reach its lethal effects and that LC 50 67.056 ppm (contact bioassay) 110.279 ppm (feeding bioassay. Thus, the developed nano-insecticides are considered effective insecticidal against M. domestica and could save the world from the vector-transmitted disease in the present environment.
合群的家蝇是致命疾病从一个地方传播到另一个地方的主要媒介。它在一些源自人类和动物的疾病和社区传播中起主要作用。由于通过化学或合成杀虫剂的根除和控制措施具有挑战性。反复接触杀虫剂产生了抗药性和不良影响。从薰衣草、柠檬、薄荷、印楝和AgNPs中提取5种不同浓度的天然植物提取物,对家蝇进行了新型杀虫剂的研究。结果表明,浸提法和投喂法处理后24 h, lc50分别为296.145 ppm(接触生物测定法)和380.187 ppm(投喂生物测定法),死亡率较高。以籼稻为原料制备的植物基纳米颗粒在最低浓度下具有良好的杀伤活性,其lc50为67.056 ppm(接触生物试验)和110.279 ppm(饲养生物试验)。因此,所开发的纳米杀虫剂被认为是对家蝇有效的杀虫剂,可以在目前的环境中拯救世界免于媒介传播疾病。
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引用次数: 0
Study of the Behavior and Entomological Parameters of Anopheles in Two Health Zones of The North-Ubangi Province, Democratic Republic of Congo 刚果民主共和国乌班吉省北部两个卫生区按蚊行为及昆虫学参数研究
Basosila Narcisse, Jean-Paul Ngbolua, M. Eric, M. Mawunu, S. Irish, M. Basimike, Nagahuedi Jonas
An entomological study was conducted using two mosquito sampling technical, pyrethrum spray catch (PSC) and Human Landing Catch (HLC), inside and outside houses in two Health Zones in North Ubangi Province give the names of Health zones . This study was conducted from 31 March to 14 April 2021. This province is located in the north of the DRC, in a forested area. The objective of this work was to identify the behavior of anopheles and their entomological parameters. In total, 784 samples of Anopheles were captured, 575 by HLC (236 inside the houses and 339 outside), and 209 by (PSC). The anopheles captured in these two environments belong to the following 3 species: Anopheles gambiae s.l, (n= 769 i.e. 98%) Anopheles funestus grp (n=10 i.e. 1%), Anopheles paludis (n=5 i.e. 1%). The behavioral study reveals that Anopheles gambiae s.l is more exophagous than endophagous with a indoors peak startings a little later between 23:00 and 02:00 hours while the outdoors peak starts a little earlier at 19:00 hours. The density of Anopheles gambiae s.l. is 98% of all Anopheles mosquitoes collected. The determination of the sporozoite index by the ELISA method gave 58% of the infested Anopheles . Anopheles gambiae s.l is the major vector of malaria in this environment and is more abundant .
在北乌班吉省两个卫生区(卫生区名称)的室内和室外进行了一项昆虫学研究,使用了两种蚊子取样技术,除虫菊喷雾捕获物(PSC)和人类着陆捕获物(HLC)。该研究于2021年3月31日至4月14日进行。该省位于刚果民主共和国北部的森林地区。这项工作的目的是确定按蚊的行为和它们的昆虫学参数。共捕获按蚊784只,hplc法捕获按蚊575只(室内236只,室外339只),PSC法捕获按蚊209只。在两种环境下捕获的按蚊分别为冈比亚按蚊(769只,占98%)、富氏按蚊(10只,占1%)和古疟蚊(5只,占1%)。行为研究表明,冈比亚按蚊的食性倾向于外食而非内食,在23:00 ~ 02:00之间,冈比亚按蚊的室内食性高峰开始时间稍晚,在19:00之间,冈比亚按蚊的室外食性高峰开始时间稍早。冈比亚按蚊密度占所采集按蚊总数的98%。ELISA法测定的孢子虫指数为58%。冈比亚按蚊(Anopheles gambiae s.l)是该地区疟疾的主要传播媒介,数量较多。
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引用次数: 0
Role of Clinical and Parasitological data in Diagnosis of Chronic Urinary Schistosomiasis in comparison with other techniques 临床和寄生虫学资料在慢性尿路血吸虫病诊断中的作用与其他技术的比较
Shaimaa H. Ali, Safeya O. El Bassiouni, Hala El Askary, K. Elesaily, D. El Akkad
The gold standard for diagnosis of schistosomiasis haematobium is a microscopic examination of urine for parasite eggs. However, direct detection of eggs is difficult among people who have chronic infections.The aim of the current study was to detect the role of clinical and parasitological examination in the diagnosis of S.haematobium in comparison with PCR in chronic patients. Materials and methods: This study was conducted on 115 urine samples; 60 samples were collected from patients with cancer bladder, 35 samples from cases of urinary schistosomiasis, and 20 samples from a healthy control. Samples enrolled in the study were subjected to patient socio-demographic history, clinical data, and the collection of histopathological reports (in the case of cancer bladder patients). All urine samples were subjected to parasitological examination for the detection of S. haematobium eggs. Multiplex PCR was used for the detection of DNA fragments of S. haematobium in urine. Results: By multiplex PCR, it was revealed that 5 cases (8.3%) were positive for S. haematobium , while 55 cases (91.7%) were negative. PCR-positive cases were in the age of 44- 67 years. Diagnosis of S. haematobium infection in symptomatic (dysuria &haematuria) suspected bilharziasis patients by using the traditional parasitological examination for detection of eggs in urine revealed absolute diagnostic efficacy (100%). S. haematobium eggs were detected in the examined patients of age group from 15 to over 40 years. Conclusion: Using clinical and direct parasitological methods in association with history taking could be enough for the diagnosis of S. haematobium, especially in aged patients.
诊断血血吸虫病的金标准是尿液中寄生虫卵的显微镜检查。然而,在慢性感染患者中,直接检测卵子是很困难的。本研究的目的是检测临床和寄生虫学检查在慢性患者中诊断血单胞菌的作用,并与PCR进行比较。材料与方法:本研究收集了115份尿样;从膀胱癌患者中采集60份样本,从尿路血吸虫病患者中采集35份样本,从健康对照中采集20份样本。纳入研究的样本接受了患者的社会人口统计学历史、临床数据和组织病理学报告(在膀胱癌患者的情况下)。所有尿样均进行寄生虫学检查,检测血氧梭菌卵。采用多重聚合酶链反应(PCR)检测尿中血氧梭菌DNA片段。结果:经多重PCR检测,5例(8.3%)血氧梭菌阳性,55例(91.7%)阴性。pcr阳性病例年龄在44 ~ 67岁之间。对有症状(排尿困难、血尿)的疑似血吸虫病患者,采用传统的寄生虫学检查检测尿中虫卵,诊断血链球菌感染的绝对诊断率为100%。在15岁至40岁以上的患者中检出血氧弧菌卵。结论:采用临床和直接的寄生虫学方法,并结合病史,足以诊断血氧梭菌,特别是老年患者。
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引用次数: 0
Assessment of Protease Enzymatic Activity Among Symptomatic Blastocystosis Patients 有症状囊胚增生患者的蛋白酶活性评估
H. Fadl, Safeya O. El Bassiouni, Hoda El Bolaky, D. Sabry, D. El Akkad
investigated the protease activity of Blastocystis isolates obtained from stool samples of symptomatic and asymptomatic individuals using gelatin SDS-PAGE and azocasein assay. The present study was carried out on 62 subjects positive for Blastocystis whether presenting with gastrointestinal symptoms or not. The symptomatic group (cases, GI) included 42 cases while the asymptomatic (control, GII) group included 20 subjects. Using gelatin SDS-PAGE analysis, the protease profiles of Blastocystis isolates showed 14 protease bands of both high and low molecular weights with significant differences between symptomatic and asymptomatic groups at 35, 60 and 140 kDa MW bands. Statistical analysis of the protease profile of Blastocystis isolates showed a significant difference (P value < 0.05) between the two groups. Using Azocasein assay, Blastocystis isolates from symptomatic cases show quantitatively higher protease activity than asymptomatic cases but without significant difference between both groups.
采用明胶SDS-PAGE和偶氮酪蛋白测定法对从有症状和无症状个体粪便样本中分离的囊虫进行蛋白酶活性研究。本研究对62名囊虫阳性受试者进行了研究,无论是否出现胃肠道症状。症状组(病例,GI) 42例,无症状组(对照组,GII) 20例。利用明胶SDS-PAGE分析,囊胚分离物的蛋白酶谱图显示出14个高分子量和低分子量的蛋白酶谱带,在35、60和140 kDa MW谱带上,症状组和无症状组之间存在显著差异。两组囊胚分离菌蛋白酶谱差异有统计学意义(P值< 0.05)。偶氮酪蛋白(azocasinin)测定结果显示,有症状的囊虫分离株的蛋白酶活性高于无症状的囊虫分离株,但两组间无显著差异。
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引用次数: 0
Parasitic Infections’ Immunomodulatory Effects and Autoimmune Diseases 寄生虫感染的免疫调节作用与自身免疫性疾病
Aliaa M Elsawey, N. Nabih, Gaballah, .M., S. Etewa, N. Aboulfotouh
The hygiene hypothesis has been implicated in the dramatic increase in autoimmune and allergic diseases noticed in recent decades, especially in developed countries. This growth was associated with lesser exposure to diverse immunoregulatory infectious agents. This hypothesis has been proved by many potent epidemiological and experimental studies. The results of these studies along with the analysis of the western world’s microbiome helped us to have a greater idea about microorganisms shared in the hygiene hypothesis, as well as their main mechanisms that have an effect on the immune system. Protozoal infections have been proved to have remarkable immunomodulatory changes in different autoimmune diseases. Helminths and their derivatives were proved to have a protective role. Helminths’ broad immunomodulatory effects have been tested in clinical trials of autoimmune diseases, including inflammatory bowel disease, multiple sclerosis, rheumatoid arthritis, and type-1 diabetes. In this review, we discussed particular parasitic infections and their immunomodulatory effects on some autoimmune diseases.
近几十年来,特别是在发达国家,自身免疫性疾病和过敏性疾病的急剧增加与卫生假说有关。这种生长与较少接触多种免疫调节感染因子有关。这一假设已被许多强有力的流行病学和实验研究证实。这些研究的结果以及对西方世界微生物组的分析帮助我们对卫生假说中共享的微生物有了更深入的了解,以及它们对免疫系统产生影响的主要机制。原生动物感染已被证明在不同的自身免疫性疾病中具有显著的免疫调节作用。蠕虫及其衍生物被证明具有保护作用。蠕虫广泛的免疫调节作用已在自身免疫性疾病的临床试验中得到检验,包括炎症性肠病、多发性硬化症、类风湿性关节炎和1型糖尿病。在这篇综述中,我们讨论了特定的寄生虫感染及其对一些自身免疫性疾病的免疫调节作用。
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引用次数: 0
Seroprevalence Study of Giardiasis Among Children with Diarrhoea in Duhok City, Kurdistan Region of Iraq 伊拉克库尔德斯坦地区杜霍克市腹泻儿童贾第虫病血清流行病学研究
A. Abdullah, Darya S. Hussein
Depending on the stool types, 3.1% (1) of infection were reported in semi-formed stool type, 7.69% (3) in loose and 5.88% (2) in watery stool type. According to the age groups, the highest infection (3) was recorded in the 6-10 years’ accounting (8.57%), while the lowest infection rate was 3.12% (1) in > 10 years’ age groups, with no significant differences. This study shows the prevalence rate of G. lamblia among children in Duhok city. Preventing this infection requires good plans and strategies with good individual hygiene, the use of a healthy drinking water supply, and useful educational program to control this disease .
根据不同的大便类型,半成型大便感染3.1%(1),稀便感染7.69%(3),水样大便感染5.88%(2)。按年龄组分,6 ~ 10岁年龄组感染率最高(3例),占8.57%;> 10岁年龄组感染率最低(1例),占3.12%,差异无统计学意义。本研究显示了杜霍克市儿童中兰螺旋体的患病率。预防这种感染需要良好的计划和策略,具有良好的个人卫生,使用健康的饮用水供应,以及有效的教育计划来控制这种疾病。
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引用次数: 0
Epidemiological Determinants of Entamoeba histolytica and Schistosoma spp. Infections in Selected Communities in Ijebu-East Local Government Area of Ogun State. 奥贡州Ijebu-East地方政府区选定社区溶组织内阿米巴原虫和血吸虫感染的流行病学决定因素
A. B., O. C., Oluwole A., Adubi O., Adeyemi O.
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引用次数: 0
Relative Abundance of the Tick Species Parasitizing Cows in Faisalabad, Pakistan 巴基斯坦费萨拉巴德地区牛寄生蜱的相对丰度
M. Bajwa, R. Maqsood, Maria Tayyaba, Muhammad Riaz, Huma Ghazal, Nargis Naheed, Muhammad Saifullah, S. Shah
are blood-feeding ectoparasites of livestock and human. Ticks can cause directly as well as indirectly losses to humans and animals all over the world. The current study was conducted to determine the abundance of tick infestation and identification of tick species in Faisalabad during 2020. Three tick species were collected belong to three genera; Hyalomma anatolicum (44.88%), Rhipicephalus microplus (30.77%) and Haemaphysalis sulcata (24.33%) . Among identified tick species, Hyalomma anatolicum was the dominant species (44.88%) followed by Rhipicephalus microplus (30.77%) and Haemaphysalis sulcata (24.33%) . Female animals were highly infested with tick species than male. Young animals carried a greater number of ticks as compared to old or adults animals. Among examined animals, 54.14% and 45.85% animals were recorded positive and negative for tick infestation, respectively.
是家畜和人类的吸血外寄生虫。蜱虫可以直接或间接地给世界各地的人类和动物造成损失。目前的研究是为了确定2020年费萨拉巴德蜱虫侵扰的丰度和蜱虫物种的鉴定。采集到蜱虫3种,隶属3属;猪眼透明瘤(44.88%)、微小棘头虫(30.77%)和短血蜱(24.33%)。在鉴定的蜱种中,以斑点透明眼蜱为优势种(44.88%),其次是微头蜱(30.77%)和短血蜱(24.33%)。雌性动物的蜱类感染率高于雄性动物。与年老或成年动物相比,幼小动物携带的蜱虫数量更多。被检动物中蜱虫感染阳性率为54.14%,阴性率为45.85%。
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引用次数: 0
期刊
Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology
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